pycom06g14420

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006447
Physical Location & Seq
Reverse (-)
19179242 .. 19180466
1225 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g14420.3

Sequence Viewer

Length: 825 bp
ATGACCATCCAGCTGAAGGAGGAGCTGATCATGAACTCCTTCAAGACAATCGACGGCCGGGGAGCGTCCGTACACATTGCCGGTGGGCCATGCATCACCATCCAGTTCGTGACCAACATTATTATCCACGGACTGCACATACACGATTGCAAGCAGGGTGGGAACGCTATGGTGAGGAGCTCCCCCAGTCACTTCGGGTGGAGGACCGTATCGGACGGTGATGGCGTGTCGATCTTCGGTGGGAGCCACGTGTGGATGGACCATTGCTCGTTGTCCAACTGCAAAGATGGGTTGGTTGATGCGATTTATGGGTCCACTGCGATAACGATTTCGAACAATTACATGACGCACCATGATAAGGTGATGCTTTTGGGGCATAGCGATTCGTATACCAACGACAAGAACATGCAAATCACCATTGCGTTCAATCACTTTGGAGAAGGCTTGGTCCAAAGAATGCCAAGATGTAGGCATGGATATTTCCATGTGGTGAACAATGACTACACCCATTGGGAGATGTATGCCATTGGTGGGAGTGCAGACCCTACAATCAATAGCCAAGGGAACAGATTTGCTGCACCAGATATCAGATCCAGCAAAGAGGTGACCAAACATGAGGATGCACCCGAAAGTGAATGGAAGAATTGGAACTGGAGGTCGGAAGGAGACTTGCTGCTCAACGGTGCGTTTTTTACGGCATCAGGTGCCGGAGCTTCCTCAAGCTACGCCAGGGCTTCGAGCTTGGGTGCAAAGCCATCTTCTCTAGTGGGTGCGATTACCACGGCTTCCGGCGCACTCAGTTGCCGGAAGGGCTCTCGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

275

Amino Acids

29.76

Weight (kDa)

6.94

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 704
AccI GTMKAC 1 cut(s) 389
AclWI GGATC 1 cut(s) 585
AcoI YGGCCR 1 cut(s) 55
AcuI CTGAAG 1 cut(s) 35
AcvI CACGTG 1 cut(s) 250
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 3 cut(s) 16, 358, 531
AflIII ACRYGT 1 cut(s) 249
AgsI TTSAA 2 cut(s) 43, 427
AjnI CCWGG 1 cut(s) 728
AluBI AGCT 6 cut(s) 13, 25, 180, 713, 723, 741
AluI AGCT 6 cut(s) 13, 25, 180, 713, 723, 741
Alw21I GWGCWC 1 cut(s) 182
Alw26I GTCTC 1 cut(s) 660
AlwI GGATC 1 cut(s) 585
AoxI GGCC 2 cut(s) 55, 86
ApeKI GCWGC 2 cut(s) 575, 673
Asp700I GAANNNNTTC 1 cut(s) 38
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 5 cut(s) 86, 204, 259, 312, 448
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 7 cut(s) 88, 184, 230, 373, 406, 502, 616
AsuII TTCGAA 1 cut(s) 332
AvaII GGWCC 4 cut(s) 204, 259, 312, 448
BanI GGYRCC 1 cut(s) 704
BanII GRGCYC 2 cut(s) 182, 815
BbrPI CACGTG 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 2 cut(s) 562, 660
BccI CCATC 6 cut(s) 14, 107, 215, 250, 281, 763
BceAI ACGGC 3 cut(s) 70, 711, 798
BciT130I CCWGG 1 cut(s) 730
BclI TGATCA 1 cut(s) 27
BcnI CCSGG 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 660
BfaI CTAG 1 cut(s) 764
BglI GCCNNNNNGGC 1 cut(s) 810
BisI GCNGC 2 cut(s) 576, 674
BlsI GCNGC 2 cut(s) 577, 675
Bme1390I CCNGG 2 cut(s) 59, 730
Bme18I GGWCC 4 cut(s) 204, 259, 312, 448
BmgT120I GGNCC 5 cut(s) 86, 204, 259, 312, 448
BmiI GGNNCC 3 cut(s) 245, 313, 706
BmrFI CCNGG 2 cut(s) 59, 730
BmrI ACTGGG 1 cut(s) 180
BmsI GCATC 5 cut(s) 102, 289, 354, 610, 707
BmuI ACTGGG 1 cut(s) 180
BpmI CTGGAG 1 cut(s) 673
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 703
BpuMI CCSGG 1 cut(s) 59
BsaAI YACGTR 1 cut(s) 250
BsaJI CCNNGG 5 cut(s) 58, 127, 559, 729, 780
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 358, 531
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 3 cut(s) 103, 186, 656
Bse3DI GCAATG 3 cut(s) 75, 262, 417
BseBI CCWGG 1 cut(s) 730
BseDI CCNNGG 5 cut(s) 58, 127, 559, 729, 780
BseGI GGATG 4 cut(s) 6, 99, 261, 625
BseLI CCNNNNNNNGG 3 cut(s) 16, 358, 531
BseMI GCAATG 3 cut(s) 75, 262, 417
BseMII CTCAG 1 cut(s) 811
BseNI ACTGG 3 cut(s) 103, 186, 656
BseRI GAGGAG 2 cut(s) 35, 190
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 2 cut(s) 562, 660
BsgI GTGCAG 3 cut(s) 119, 558, 561
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 2 cut(s) 57, 88
BshNI GGYRCC 1 cut(s) 704
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 182
BsiSI CCGG 5 cut(s) 58, 81, 708, 789, 805
BslI CCNNNNNNNGG 3 cut(s) 16, 358, 531
BsmAI GTCTC 1 cut(s) 660
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 2 cut(s) 57, 88
Bsp119I TTCGAA 1 cut(s) 332
Bsp1286I GDGCHC 2 cut(s) 182, 815
Bsp143I GATC 3 cut(s) 27, 231, 590
BspANI GGCC 2 cut(s) 57, 88
BspCNI CTCAG 1 cut(s) 810
BspHI TCATGA 1 cut(s) 30
BspLI GGNNCC 3 cut(s) 245, 313, 706
BspPI GGATC 1 cut(s) 585
BspT104I TTCGAA 1 cut(s) 332
BspT107I GGYRCC 1 cut(s) 704
BsrDI GCAATG 3 cut(s) 75, 262, 417
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 3 cut(s) 103, 186, 656
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 5 cut(s) 58, 127, 559, 729, 780
BssMI GATC 3 cut(s) 27, 231, 590
BssNAI GTATAC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 559
Bst1107I GTATAC 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 730
Bst4CI ACNGT 3 cut(s) 208, 218, 683
BstAPI GCANNNNNTGC 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 250
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 152
BstDEI CTNAG 1 cut(s) 797
BstDSI CCRYGG 2 cut(s) 127, 780
BstEII GGTNACC 1 cut(s) 604
BstF5I GGATG 4 cut(s) 6, 99, 261, 625
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 3 cut(s) 30, 234, 593
BstMAI GTCTC 1 cut(s) 660
BstMBI GATC 3 cut(s) 27, 231, 590
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 5 cut(s) 373, 704, 791, 810, 819
BstNI CCWGG 1 cut(s) 730
BstNSI RCATGY 1 cut(s) 409
BstPI GGTNACC 1 cut(s) 604
BstSCI CCNGG 2 cut(s) 57, 728
BstV1I GCAGC 2 cut(s) 562, 660
BstX2I RGATCY 1 cut(s) 590
BstYI RGATCY 1 cut(s) 590
BstZ17I GTATAC 1 cut(s) 390
BstZI CGGCCG 1 cut(s) 55
BsuRI GGCC 2 cut(s) 57, 88
BtgI CCRYGG 2 cut(s) 127, 780
BtsCI GGATG 4 cut(s) 6, 99, 261, 625
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 1 cut(s) 152
CciI TCATGA 1 cut(s) 30
CfoI GCGC 1 cut(s) 794
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 5 cut(s) 86, 204, 259, 312, 448
CseI GACGC 2 cut(s) 54, 355
Csp6I GTAC 1 cut(s) 71
CspCI CAANNNNNGTGG 2 cut(s) 139, 174
CviAII CATG 8 cut(s) 31, 90, 343, 353, 406, 473, 485, 614
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 1 cut(s) 797
DpnI GATC 3 cut(s) 29, 233, 592
DpnII GATC 3 cut(s) 27, 231, 590
EaeI YGGCCR 1 cut(s) 55
EagI CGGCCG 1 cut(s) 55
Ecl136II GAGCTC 1 cut(s) 180
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 559
Eco24I GRGCYC 2 cut(s) 182, 815
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 4 cut(s) 204, 259, 312, 448
Eco52I CGGCCG 1 cut(s) 55
Eco53kI GAGCTC 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 35
Eco72I CACGTG 1 cut(s) 250
Eco91I GGTNACC 1 cut(s) 604
EcoICRI GAGCTC 1 cut(s) 180
EcoO65I GGTNACC 1 cut(s) 604
EcoRII CCWGG 1 cut(s) 728
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 1 cut(s) 559
EcoT22I ATGCAT 1 cut(s) 95
EcoT38I GRGCYC 2 cut(s) 182, 815
ErhI CCWWGG 1 cut(s) 559
FaeI CATG 8 cut(s) 34, 93, 346, 356, 409, 476, 488, 617
FatI CATG 8 cut(s) 30, 89, 342, 352, 405, 472, 484, 613
FbaI TGATCA 1 cut(s) 27
FblI GTMKAC 1 cut(s) 389
Fnu4HI GCNGC 2 cut(s) 576, 674
FokI GGATG 3 cut(s) 86, 268, 632
FriOI GRGCYC 2 cut(s) 182, 815
Fsp4HI GCNGC 2 cut(s) 576, 674
FspBI CTAG 1 cut(s) 764
GlaI GCGC 1 cut(s) 793
GluI GCNGC 2 cut(s) 576, 674
GsuI CTGGAG 1 cut(s) 673
HaeIII GGCC 2 cut(s) 57, 88
HapII CCGG 5 cut(s) 58, 81, 708, 789, 805
HgaI GACGC 2 cut(s) 54, 355
HhaI GCGC 1 cut(s) 794
Hin1II CATG 8 cut(s) 34, 93, 346, 356, 409, 476, 488, 617
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HinfI GANTC 1 cut(s) 383
HpaII CCGG 5 cut(s) 58, 81, 708, 789, 805
HphI GGTGA 7 cut(s) 88, 184, 230, 373, 406, 502, 616
Hpy166II GTNNAC 4 cut(s) 73, 315, 390, 493
Hpy188I TCNGA 3 cut(s) 214, 590, 661
Hpy188III TCNNGA 3 cut(s) 31, 43, 109
Hpy8I GTNNAC 4 cut(s) 73, 315, 390, 493
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 5 cut(s) 10, 49, 434, 656, 802
HpyCH4III ACNGT 3 cut(s) 208, 218, 683
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 9 cut(s) 93, 136, 150, 282, 409, 539, 578, 623, 749
HpyF10VI GCNNNNNNNGC 5 cut(s) 373, 704, 791, 810, 819
HpyF3I CTNAG 1 cut(s) 797
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 8 cut(s) 34, 93, 346, 356, 409, 476, 488, 617
HspAI GCGC 1 cut(s) 792
Ksp22I TGATCA 1 cut(s) 27
Kzo9I GATC 3 cut(s) 27, 231, 590
LmnI GCTCC 6 cut(s) 22, 62, 177, 185, 243, 710
Lsp1109I GCAGC 2 cut(s) 562, 660
LweI GCATC 5 cut(s) 102, 289, 354, 610, 707
MaeI CTAG 1 cut(s) 764
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 3 cut(s) 109, 188, 604
MalI GATC 3 cut(s) 29, 233, 592
MboI GATC 3 cut(s) 27, 231, 590
MboII GAAGA 3 cut(s) 226, 652, 750
MflI RGATCY 1 cut(s) 590
MhlI GDGCHC 2 cut(s) 182, 815
MluCI AATT 2 cut(s) 337, 643
MmeI TCCRAC 2 cut(s) 300, 639
MnlI CCTC 7 cut(s) 13, 168, 195, 595, 610, 648, 727
Mph1103I ATGCAT 1 cut(s) 95
MroXI GAANNNNTTC 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 618
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 5 cut(s) 58, 81, 708, 789, 805
MspR9I CCNGG 2 cut(s) 59, 730
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 1 cut(s) 730
MwoI GCNNNNNNNGC 5 cut(s) 373, 704, 791, 810, 819
NciI CCSGG 1 cut(s) 59
NdeII GATC 3 cut(s) 27, 231, 590
NlaIII CATG 8 cut(s) 34, 93, 346, 356, 409, 476, 488, 617
NlaIV GGNNCC 3 cut(s) 245, 313, 706
NmuCI GTSAC 3 cut(s) 109, 188, 604
NsiI ATGCAT 1 cut(s) 95
NspI RCATGY 1 cut(s) 409
NspV TTCGAA 1 cut(s) 332
PagI TCATGA 1 cut(s) 30
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 462
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 383
PkrI GCNGC 2 cut(s) 577, 675
PmaCI CACGTG 1 cut(s) 250
PmlI CACGTG 1 cut(s) 250
Ppu21I YACGTR 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 728
PspCI CACGTG 1 cut(s) 250
PspEI GGTNACC 1 cut(s) 604
PspGI CCWGG 1 cut(s) 728
PspN4I GGNNCC 3 cut(s) 245, 313, 706
PspPI GGNCC 5 cut(s) 86, 204, 259, 312, 448
PsrI GAACNNNNNNTAC 2 cut(s) 485, 517
PsuI RGATCY 1 cut(s) 590
PvuII CAGCTG 1 cut(s) 13
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
RseI CAYNNNNRTG 1 cut(s) 618
SacI GAGCTC 1 cut(s) 182
SatI GCNGC 2 cut(s) 576, 674
Sau3AI GATC 3 cut(s) 27, 231, 590
Sau96I GGNCC 5 cut(s) 86, 204, 259, 312, 448
ScrFI CCNGG 2 cut(s) 59, 730
SduI GDGCHC 2 cut(s) 182, 815
SfaNI GCATC 5 cut(s) 102, 289, 354, 610, 707
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 4 cut(s) 204, 259, 312, 448
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 1 cut(s) 718
SmoI CTYRAG 1 cut(s) 718
Sse9I AATT 2 cut(s) 337, 643
SspMI CTAG 1 cut(s) 764
SstI GAGCTC 1 cut(s) 182
StyD4I CCNGG 2 cut(s) 57, 728
StyI CCWWGG 1 cut(s) 559
TaaI ACNGT 3 cut(s) 208, 218, 683
TaiI ACGT 1 cut(s) 252
TaqI TCGA 4 cut(s) 51, 230, 332, 737
TasI AATT 2 cut(s) 337, 643
TfiI GAWTC 1 cut(s) 383
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 3 cut(s) 109, 188, 604
TseI GCWGC 2 cut(s) 575, 673
Tsp45I GTSAC 3 cut(s) 109, 188, 604
TspDTI ATGAA 1 cut(s) 47
TspGWI ACGGA 2 cut(s) 58, 144
TspRI CASTG 1 cut(s) 322
VpaK11BI GGWCC 4 cut(s) 204, 259, 312, 448
XceI RCATGY 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 389
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 1 cut(s) 764
Zsp2I ATGCAT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.