Rh7AG121900

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006447
Physical Location & Seq
Reverse (-)
9552798 .. 9557957
5160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG121900.1

Sequence Viewer

Length: 1278 bp
ATGGCTCAAGTACTGGACCAGTTGGATGCAGCTTTCGTATCATCGTCTAGTTTGCAGGGTGGGAAGCTCCATCAAGAACTTACATCAATGCCTAATATAAGACCACCATATATTGCCCCACTGGAACATGTTCAAGATCCTGAACTCGTTGTACAGCAAGTACAAAGGAGCATCTATAATGCCTCAGTTACCCGGAGGAAGTTGGGCTATTTGTCATGTGGGTCCGGAAACCCGATCGACGACTGCTGGCGGTGCGACCCGAATTGGGAGAAGAACCGCCAGAGGCTAGCCGACTGCGCAATTGGGTTCGGCAAAAACGCCATTGGCGGCAGGGACGGGAGGATTTACGTGGTGACTGACTCAGGGGACGACGACCCCGTGAACCCCAAGCCAGGGACTCTACGACATGCCGTCATCCAGGACGAGCCCTTGTGGATCATTTTCGAGCGCGACATGTCGATCAAGCTCAAGGAGGAGCTGATCATGAACTCGTTCAAGACCCTCGACGGCCGAGGAGCCAGCGTGCACATCTCCGGCGGGCCATGCATTACGATCCAGTACGTGACCAACATTATCATACATGGACTCAACATACACGATTGCAAGCCGGGAGGGAATGCTATGGTGCGTGACTCCCCAAATCACTTCGGGTGGAGAACCATATCGGACGGTGATGGTGTGTCCATCTTCGGTGGGAGCCATGTTTGGGTGGACCATTGCTCCCTGTCGAACTGCGCCGACGGGCTGGTCGACGCAATTCATGGTTCCACTGCGATAACGATTTCGAACAATTACATGACGCACCATGATAAGGTCATGTTGCTGGGACACAGTGATACCTATACCCAGGACAAGAACATGCAGGTCACTATTGCCTTCAACCACTTTGGAGAAGGTCTTGTTCAAAGGATGCCAAGATGTAGGCATGGATATTTCCATGTGGTGAACAATGACTACACCCATTGGGAGATGTATGCCATTGGAGGCAGTGCAAACCCTACCATCAATAGCCAGGGGAACAGGTTTACTGCACCAGATATAAGGTTCAGCAAAGAGGTGACAAAACATGAGGATGCACCTGAAAATGAGTGGAAAAATTGGAACTGGAGGTCAGAGGGTGACATGTTGATAAACGGTGCATTTTTCACGGCCTCTGGTGCCGGAGCTTCTTCAAGCTATGCAAAAGCCTCAAGCTTGGGGGCAAGGCCATCTTCTCTTGTGGCTTCAATCACCACAACTTCTGGTGCACTCAATTGCCGAAAGGGTTCTCGTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

425

Amino Acids

46.72

Weight (kDa)

6.4

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 222 - 357 3e-20 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 746
Acc16I TGCGCA 1 cut(s) 298
Acc36I ACCTGC 1 cut(s) 853
AccB1I GGYRCC 1 cut(s) 1157
AccI GTMKAC 1 cut(s) 750
AccII CGCG 1 cut(s) 450
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 4 cut(s) 250, 277, 327, 537
AclWI GGATC 3 cut(s) 131, 443, 547
AcoI YGGCCR 1 cut(s) 508
AfaI GTAC 4 cut(s) 12, 153, 162, 560
AfiI CCNNNNNNNGG 5 cut(s) 265, 392, 393, 706, 811
AflIII ACRYGT 3 cut(s) 127, 453, 1122
AgsI TTSAA 6 cut(s) 134, 496, 880, 905, 1173, 1227
AhdI GACNNNNNGTC 1 cut(s) 410
AjnI CCWGG 4 cut(s) 391, 417, 846, 1011
AluBI AGCT 7 cut(s) 32, 67, 466, 478, 1166, 1176, 1194
AluI AGCT 7 cut(s) 32, 67, 466, 478, 1166, 1176, 1194
Alw21I GWGCWC 2 cut(s) 528, 1249
Alw44I GTGCAC 2 cut(s) 524, 1245
AlwI GGATC 3 cut(s) 131, 443, 547
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 4 cut(s) 508, 539, 1149, 1205
ApaLI GTGCAC 2 cut(s) 524, 1245
ApeKI GCWGC 1 cut(s) 29
Asp700I GAANNNNTTC 3 cut(s) 129, 491, 1264
AspLEI GCGC 3 cut(s) 299, 450, 737
AspS9I GGNCC 4 cut(s) 16, 222, 539, 712
AsuC2I CCSGG 2 cut(s) 193, 609
AsuHPI GGTGA 6 cut(s) 364, 683, 955, 1069, 1130, 1222
AsuII TTCGAA 1 cut(s) 785
AsuNHI GCTAGC 1 cut(s) 286
AvaII GGWCC 3 cut(s) 16, 222, 712
BaeGI GKGCMC 2 cut(s) 528, 1249
BanI GGYRCC 1 cut(s) 1157
BanII GRGCYC 1 cut(s) 429
Bbv12I GWGCWC 2 cut(s) 528, 1249
BbvI GCAGC 1 cut(s) 41
BccI CCATC 5 cut(s) 78, 668, 692, 1010, 1216
BceAI ACGGC 3 cut(s) 395, 523, 1164
BciT130I CCWGG 4 cut(s) 393, 419, 848, 1013
BclI TGATCA 1 cut(s) 480
BcnI CCSGG 2 cut(s) 193, 609
BfaI CTAG 2 cut(s) 48, 287
BfuAI ACCTGC 1 cut(s) 853
BisI GCNGC 2 cut(s) 30, 328
BlsI GCNGC 2 cut(s) 31, 329
BmcAI AGTACT 1 cut(s) 12
Bme1390I CCNGG 6 cut(s) 193, 393, 419, 609, 848, 1013
Bme18I GGWCC 3 cut(s) 16, 222, 712
BmeRI GACNNNNNGTC 1 cut(s) 410
BmgT120I GGNCC 4 cut(s) 16, 222, 539, 712
BmiI GGNNCC 5 cut(s) 223, 517, 698, 766, 1159
BmrFI CCNGG 6 cut(s) 193, 393, 419, 609, 848, 1013
BmsI GCATC 4 cut(s) 16, 180, 900, 1063
BmtI GCTAGC 1 cut(s) 290
BpmI CTGGAG 1 cut(s) 1126
Bpu14I TTCGAA 1 cut(s) 785
BpuEI CTTGAG 2 cut(s) 452, 1174
BpuMI CCSGG 2 cut(s) 193, 609
BsaAI YACGTR 2 cut(s) 349, 562
BsaJI CCNNGG 4 cut(s) 392, 511, 846, 1012
BsaWI WCCGGW 1 cut(s) 224
BsaXI ACNNNNNCTCC 4 cut(s) 507, 537, 704, 734
Bsc4I CCNNNNNNNGG 5 cut(s) 265, 392, 393, 706, 811
Bse1I ACTGG 5 cut(s) 18, 19, 126, 556, 1109
Bse3DI GCAATG 1 cut(s) 715
BseAI TCCGGA 1 cut(s) 224
BseBI CCWGG 4 cut(s) 393, 419, 848, 1013
BseDI CCNNGG 4 cut(s) 392, 511, 846, 1012
BseGI GGATG 4 cut(s) 31, 414, 915, 1078
BseLI CCNNNNNNNGG 5 cut(s) 265, 392, 393, 706, 811
BseMI GCAATG 1 cut(s) 715
BseMII CTCAG 2 cut(s) 198, 375
BseNI ACTGG 5 cut(s) 18, 19, 126, 556, 1109
BseRI GAGGAG 2 cut(s) 488, 528
BseSI GKGCMC 2 cut(s) 528, 1249
BseX3I CGGCCG 1 cut(s) 508
BseXI GCAGC 1 cut(s) 41
BseYI CCCAGC 1 cut(s) 823
BsgI GTGCAG 1 cut(s) 1014
Bsh1236I CGCG 1 cut(s) 450
Bsh1285I CGRYCG 2 cut(s) 237, 511
BshFI GGCC 4 cut(s) 510, 541, 1151, 1207
BshNI GGYRCC 1 cut(s) 1157
BsiEI CGRYCG 2 cut(s) 237, 511
BsiHKAI GWGCWC 2 cut(s) 528, 1249
BsiSI CCGG 5 cut(s) 193, 225, 534, 608, 1161
BslFI GGGAC 4 cut(s) 347, 380, 409, 840
BslI CCNNNNNNNGG 5 cut(s) 265, 392, 393, 706, 811
BsmFI GGGAC 4 cut(s) 347, 380, 409, 840
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 4 cut(s) 510, 541, 1151, 1207
Bsp119I TTCGAA 1 cut(s) 785
Bsp1286I GDGCHC 3 cut(s) 429, 528, 1249
Bsp13I TCCGGA 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 151
Bsp143I GATC 6 cut(s) 136, 234, 435, 459, 480, 552
BspACI CCGC 4 cut(s) 250, 277, 327, 537
BspANI GGCC 4 cut(s) 510, 541, 1151, 1207
BspCNI CTCAG 2 cut(s) 197, 374
BspEI TCCGGA 1 cut(s) 224
BspFNI CGCG 1 cut(s) 450
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 5 cut(s) 223, 517, 698, 766, 1159
BspMI ACCTGC 1 cut(s) 853
BspOI GCTAGC 1 cut(s) 290
BspPI GGATC 3 cut(s) 131, 443, 547
BspT104I TTCGAA 1 cut(s) 785
BspT107I GGYRCC 1 cut(s) 1157
BsrDI GCAATG 1 cut(s) 715
BsrGI TGTACA 1 cut(s) 151
BsrI ACTGG 5 cut(s) 18, 19, 126, 556, 1109
BssECI CCNNGG 4 cut(s) 392, 511, 846, 1012
BssMI GATC 6 cut(s) 136, 234, 435, 459, 480, 552
Bst2UI CCWGG 4 cut(s) 393, 419, 848, 1013
Bst4CI ACNGT 3 cut(s) 671, 833, 1136
BstAUI TGTACA 1 cut(s) 151
BstBAI YACGTR 2 cut(s) 349, 562
BstBI TTCGAA 1 cut(s) 785
BstC8I GCNNGC 6 cut(s) 248, 288, 520, 524, 539, 605
BstDEI CTNAG 2 cut(s) 184, 361
BstF5I GGATG 4 cut(s) 31, 414, 915, 1078
BstFNI CGCG 1 cut(s) 450
BstHHI GCGC 3 cut(s) 299, 450, 737
BstKTI GATC 6 cut(s) 139, 237, 438, 462, 483, 555
BstMBI GATC 6 cut(s) 136, 234, 435, 459, 480, 552
BstMCI CGRYCG 2 cut(s) 237, 511
BstMWI GCNNNNNNNGC 4 cut(s) 252, 296, 543, 1157
BstNI CCWGG 4 cut(s) 393, 419, 848, 1013
BstNSI RCATGY 5 cut(s) 131, 410, 457, 862, 1126
BstSCI CCNGG 6 cut(s) 191, 391, 417, 607, 846, 1011
BstSLI GKGCMC 2 cut(s) 528, 1249
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BstZI CGGCCG 1 cut(s) 508
BsuRI GGCC 4 cut(s) 510, 541, 1151, 1207
BtsCI GGATG 4 cut(s) 31, 414, 915, 1078
BtsI GCAGTG 2 cut(s) 768, 994
BtsIMutI CAGTG 4 cut(s) 119, 768, 838, 994
BveI ACCTGC 1 cut(s) 853
Cac8I GCNNGC 6 cut(s) 248, 288, 520, 524, 539, 605
CciI TCATGA 1 cut(s) 483
CfoI GCGC 3 cut(s) 299, 450, 737
Cfr13I GGNCC 4 cut(s) 16, 222, 539, 712
CseI GACGC 2 cut(s) 761, 808
Csp6I GTAC 4 cut(s) 11, 152, 161, 559
CviQI GTAC 4 cut(s) 11, 152, 161, 559
DdeI CTNAG 2 cut(s) 184, 361
DpnI GATC 6 cut(s) 138, 236, 437, 461, 482, 554
DpnII GATC 6 cut(s) 136, 234, 435, 459, 480, 552
DrdI GACNNNNNNGTC 1 cut(s) 746
DriI GACNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 746
EaeI YGGCCR 1 cut(s) 508
EagI CGGCCG 1 cut(s) 508
Eam1105I GACNNNNNGTC 1 cut(s) 410
EclXI CGGCCG 1 cut(s) 508
Eco24I GRGCYC 1 cut(s) 429
Eco47I GGWCC 3 cut(s) 16, 222, 712
Eco52I CGGCCG 1 cut(s) 508
EcoRII CCWGG 4 cut(s) 391, 417, 846, 1011
EcoT22I ATGCAT 1 cut(s) 548
EcoT38I GRGCYC 1 cut(s) 429
FalI AAGNNNNNCTT 2 cut(s) 1195, 1227
FaqI GGGAC 4 cut(s) 347, 380, 409, 840
FauI CCCGC 1 cut(s) 530
FbaI TGATCA 1 cut(s) 480
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 2 cut(s) 30, 328
FokI GGATG 4 cut(s) 38, 401, 922, 1085
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 2 cut(s) 30, 328
FspBI CTAG 2 cut(s) 48, 287
FspI TGCGCA 1 cut(s) 298
GlaI GCGC 3 cut(s) 298, 449, 736
GluI GCNGC 2 cut(s) 30, 328
GsaI CCCAGC 1 cut(s) 827
GsuI CTGGAG 1 cut(s) 1126
HaeIII GGCC 4 cut(s) 510, 541, 1151, 1207
HapII CCGG 5 cut(s) 193, 225, 534, 608, 1161
HgaI GACGC 2 cut(s) 761, 808
HhaI GCGC 3 cut(s) 299, 450, 737
Hin6I GCGC 3 cut(s) 297, 448, 735
HinP1I GCGC 3 cut(s) 297, 448, 735
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HindIII AAGCTT 1 cut(s) 1192
HinfI GANTC 4 cut(s) 359, 397, 585, 632
HpaII CCGG 5 cut(s) 193, 225, 534, 608, 1161
HphI GGTGA 6 cut(s) 364, 683, 955, 1069, 1130, 1222
Hpy166II GTNNAC 7 cut(s) 382, 526, 712, 751, 946, 1026, 1247
Hpy188I TCNGA 2 cut(s) 667, 1114
Hpy188III TCNNGA 6 cut(s) 74, 134, 140, 225, 484, 496
Hpy8I GTNNAC 7 cut(s) 382, 526, 712, 751, 946, 1026, 1247
Hpy99I CGWCG 5 cut(s) 242, 374, 509, 743, 755
HpyAV CCTTC 2 cut(s) 886, 887
HpyCH4III ACNGT 3 cut(s) 671, 833, 1136
HpyCH4IV ACGT 2 cut(s) 348, 561
HpyF10VI GCNNNNNNNGC 4 cut(s) 252, 296, 543, 1157
HpyF3I CTNAG 2 cut(s) 184, 361
HpySE526I ACGT 2 cut(s) 348, 561
HspAI GCGC 3 cut(s) 297, 448, 735
Kpn2I TCCGGA 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 480
Kzo9I GATC 6 cut(s) 136, 234, 435, 459, 480, 552
LmnI GCTCC 7 cut(s) 72, 168, 475, 515, 696, 725, 1163
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 4 cut(s) 16, 180, 900, 1063
MaeI CTAG 2 cut(s) 48, 287
MaeII ACGT 2 cut(s) 348, 561
MaeIII GTNAC 7 cut(s) 187, 352, 562, 629, 865, 1057, 1118
MalI GATC 6 cut(s) 138, 236, 437, 461, 482, 554
MboI GATC 6 cut(s) 136, 234, 435, 459, 480, 552
MboII GAAGA 4 cut(s) 283, 679, 1161, 1203
MfeI CAATTG 2 cut(s) 300, 1252
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 3 cut(s) 429, 528, 1249
MluCI AATT 6 cut(s) 262, 300, 756, 790, 1096, 1252
MlyI GAGTC 4 cut(s) 353, 391, 579, 626
Mph1103I ATGCAT 1 cut(s) 548
MroI TCCGGA 1 cut(s) 224
MroXI GAANNNNTTC 3 cut(s) 129, 491, 1264
MslI CAYNNNNRTG 1 cut(s) 1071
MspI CCGG 5 cut(s) 193, 225, 534, 608, 1161
MspR9I CCNGG 6 cut(s) 193, 393, 419, 609, 848, 1013
MunI CAATTG 2 cut(s) 300, 1252
Mva1269I GAATGC 1 cut(s) 622
MvaI CCWGG 4 cut(s) 393, 419, 848, 1013
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 4 cut(s) 252, 296, 543, 1157
NciI CCSGG 2 cut(s) 193, 609
NdeII GATC 6 cut(s) 136, 234, 435, 459, 480, 552
NheI GCTAGC 1 cut(s) 286
NlaIV GGNNCC 5 cut(s) 223, 517, 698, 766, 1159
NmeAIII GCCGAG 1 cut(s) 536
NmuCI GTSAC 6 cut(s) 352, 562, 629, 865, 1057, 1118
NsbI TGCGCA 1 cut(s) 298
NsiI ATGCAT 1 cut(s) 548
NspI RCATGY 5 cut(s) 131, 410, 457, 862, 1126
NspV TTCGAA 1 cut(s) 785
PagI TCATGA 1 cut(s) 483
PciI ACATGT 3 cut(s) 127, 453, 1122
PcsI WCGNNNNNNNCGW 2 cut(s) 375, 747
PctI GAATGC 1 cut(s) 622
PdmI GAANNNNTTC 3 cut(s) 129, 491, 1264
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 2 cut(s) 31, 329
Ple19I CGATCG 1 cut(s) 237
PleI GAGTC 4 cut(s) 353, 391, 579, 626
PpsI GAGTC 4 cut(s) 353, 391, 579, 626
Ppu21I YACGTR 2 cut(s) 349, 562
PscI ACATGT 3 cut(s) 127, 453, 1122
Psp6I CCWGG 4 cut(s) 391, 417, 846, 1011
PspFI CCCAGC 1 cut(s) 823
PspGI CCWGG 4 cut(s) 391, 417, 846, 1011
PspN4I GGNNCC 5 cut(s) 223, 517, 698, 766, 1159
PspPI GGNCC 4 cut(s) 16, 222, 539, 712
PsrI GAACNNNNNNTAC 4 cut(s) 135, 167, 938, 970
PsuI RGATCY 1 cut(s) 136
PvuI CGATCG 1 cut(s) 237
RsaI GTAC 4 cut(s) 12, 153, 162, 560
RsaNI GTAC 4 cut(s) 11, 152, 161, 559
RseI CAYNNNNRTG 1 cut(s) 1071
SalI GTCGAC 1 cut(s) 749
SatI GCNGC 2 cut(s) 30, 328
Sau3AI GATC 6 cut(s) 136, 234, 435, 459, 480, 552
Sau96I GGNCC 4 cut(s) 16, 222, 539, 712
ScaI AGTACT 1 cut(s) 12
SchI GAGTC 4 cut(s) 353, 391, 579, 626
ScrFI CCNGG 6 cut(s) 193, 393, 419, 609, 848, 1013
SduI GDGCHC 3 cut(s) 429, 528, 1249
SfaNI GCATC 4 cut(s) 16, 180, 900, 1063
SfuI TTCGAA 1 cut(s) 785
SinI GGWCC 3 cut(s) 16, 222, 712
SmiMI CAYNNNNRTG 1 cut(s) 1071
SmlI CTYRAG 3 cut(s) 6, 467, 1189
SmoI CTYRAG 3 cut(s) 6, 467, 1189
Sse9I AATT 6 cut(s) 262, 300, 756, 790, 1096, 1252
SsiI CCGC 4 cut(s) 250, 277, 327, 537
SspMI CTAG 2 cut(s) 48, 287
StyD4I CCNGG 6 cut(s) 191, 391, 417, 607, 846, 1011
TaaI ACNGT 3 cut(s) 671, 833, 1136
TaiI ACGT 2 cut(s) 351, 564
TaqI TCGA 7 cut(s) 237, 444, 458, 504, 728, 750, 785
TasI AATT 6 cut(s) 262, 300, 756, 790, 1096, 1252
TatI WGTACW 3 cut(s) 10, 151, 160
TauI GCSGC 1 cut(s) 330
TscAI CASTG 4 cut(s) 126, 775, 838, 994
TseFI GTSAC 6 cut(s) 352, 562, 629, 865, 1057, 1118
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 6 cut(s) 352, 562, 629, 865, 1057, 1118
TspDTI ATGAA 2 cut(s) 500, 749
TspRI CASTG 4 cut(s) 126, 775, 838, 994
VneI GTGCAC 2 cut(s) 524, 1245
VpaK11BI GGWCC 3 cut(s) 16, 222, 712
XceI RCATGY 5 cut(s) 131, 410, 457, 862, 1126
XmiI GTMKAC 1 cut(s) 750
XmnI GAANNNNTTC 3 cut(s) 129, 491, 1264
XspI CTAG 2 cut(s) 48, 287
ZrmI AGTACT 1 cut(s) 12
Zsp2I ATGCAT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.