MD06G1161400.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006447
Physical Location & Seq
Reverse (-)
30244768 .. 30247075
2308 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1161400.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGCCAAAAATGCCAAGGCCCTCCTCAGGCCCCTCACTTCTCTCTCCCCTCCTCCTCCTCCCTCTCCTCTCTCTCCTCTCCCCAACCCTCATTTCCTCCAGGCCACTTCATCTCCAAGACCCTGAATTGGTAGTACAAGAGGTACAAAGGAATATTAGCGACTCAGTATCTAGGAGGAACTTGGGCTACTTGTCATGCGGGACCGGCAACCCTATCGACGACTGCTGGCGGTGCGACCCGAACTGGGAGAAGAACAGGCAGAGCTTAGCTGATTGTGCGATAGGGTTCGGAAAGAACGCCATAGGTGGAAGAGACGGGAAGATTTACGTGGTCACAGATTCCGGCGATGACGACCCCGTGAACCCCAAGCCAGGAACCCTACGACACGCCGTCATCCAAGACGAGCCATTATGGATCATTTTCCAGCGTGACATGACCATCCAGCTGAAGGAGGAGCTGATCATGAACTCCTTCAAGACAATCGACGGCCGGGGAGCGTCCGTACACATTGCCGGCGGGCCATGCATCACCATCCAGTTCGTGACCAACATTATTATCCACGGACTGCACATACACGATTGCAAGCAGGGTGGGAACGCTATGGTGAGGAGCTCCCCCAGGCACTTCGGGTGGAGGACCGTATCGGACGGCGACGGCGTGTCGATCTTCGGTGGGAGCCACGTGTGGGTGGACCATTGCTCGTTGTCCAACTGCAAAGATGGGTTGGTTGATGCAATTTATGGGTCCACTGCGATAACGATTTCGAACAATTACATGACGCACCATGATAAGGTGATGCTTTTGGGGCATAGCGATTCGTATACCAACGACAAGAACATGCAAATCACCATTGCGTTCAATCACTTTGGAGAAGGCTTGGTCCAAAGAATGCCAAGATGTAGGCATGGATATTTCCATGTGGTGAACAATGACTACACCCATTGGGAGATGTATGCCATTGGTGGGAGTGCAGACCCTACAATCAATAGCCAAGGGAACAGATTTGCTGCACCAGATATCAGATCCAGCAAAGAGGTGACCAAACATGAGGATGCACCAGAAAGTGAATGGAAGAATTGGAACTGGAGGTCGGAAGGCGACTTGATGCTCAACGGTGCGTTTTTTACTGCATCAGGTGCCGGAGCTTCCTCTAGCTACGCCAGGGCTTCGAGCTTGGGTGCAAAGCCATCTTCTCTAGTGGGTGCGATTACCACGGCTTCCGGCGCACTTAGTTGCCGAAAGGGCTCTCGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

419

Amino Acids

45.68

Weight (kDa)

6.78

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 156 - 351 2.2e-20 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1136
AccI GTMKAC 1 cut(s) 821
AciI CCGC 3 cut(s) 198, 229, 516
AclWI GGATC 2 cut(s) 422, 1017
AcoI YGGCCR 1 cut(s) 487
AcuI CTGAAG 1 cut(s) 467
AcvI CACGTG 1 cut(s) 682
AfaI GTAC 3 cut(s) 135, 144, 504
AfiI CCNNNNNNNGG 8 cut(s) 26, 127, 244, 371, 448, 685, 790, 963
AflIII ACRYGT 1 cut(s) 681
AgsI TTSAA 2 cut(s) 475, 859
AhdI GACNNNNNGTC 2 cut(s) 389, 658
AjnI CCWGG 4 cut(s) 98, 370, 617, 1160
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AluBI AGCT 8 cut(s) 264, 269, 445, 457, 612, 1145, 1155, 1173
AluI AGCT 8 cut(s) 264, 269, 445, 457, 612, 1145, 1155, 1173
Alw21I GWGCWC 1 cut(s) 614
Alw26I GTCTC 1 cut(s) 306
AlwI GGATC 2 cut(s) 422, 1017
AoxI GGCC 5 cut(s) 17, 28, 101, 487, 518
ApeKI GCWGC 1 cut(s) 1007
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 1226
AspS9I GGNCC 8 cut(s) 18, 29, 201, 518, 636, 691, 744, 880
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 6 cut(s) 520, 616, 805, 838, 934, 1048
AsuII TTCGAA 1 cut(s) 764
AvaII GGWCC 5 cut(s) 201, 636, 691, 744, 880
AxyI CCTNAGG 1 cut(s) 25
BanI GGYRCC 1 cut(s) 1136
BanII GRGCYC 2 cut(s) 614, 1247
BbrPI CACGTG 1 cut(s) 682
Bbv12I GWGCWC 1 cut(s) 614
BbvI GCAGC 1 cut(s) 994
BccI CCATC 4 cut(s) 446, 539, 713, 1195
BceAI ACGGC 5 cut(s) 374, 502, 664, 670, 1230
BcgI CGANNNNNNTGC 2 cut(s) 196, 230
BciT130I CCWGG 4 cut(s) 100, 372, 619, 1162
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 1 cut(s) 306
BfaI CTAG 3 cut(s) 171, 1152, 1196
BglI GCCNNNNNGGC 1 cut(s) 1242
BisI GCNGC 1 cut(s) 1008
BlpI GCTNAGC 1 cut(s) 265
BlsI GCNGC 1 cut(s) 1009
Bme1390I CCNGG 5 cut(s) 100, 372, 491, 619, 1162
Bme18I GGWCC 5 cut(s) 201, 636, 691, 744, 880
BmeRI GACNNNNNGTC 2 cut(s) 389, 658
BmgT120I GGNCC 8 cut(s) 18, 29, 201, 518, 636, 691, 744, 880
BmiI GGNNCC 6 cut(s) 31, 202, 376, 677, 745, 1138
BmrFI CCNGG 5 cut(s) 100, 372, 491, 619, 1162
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 6 cut(s) 534, 721, 786, 1042, 1095, 1139
BmuI ACTGGG 1 cut(s) 253
BpmI CTGGAG 2 cut(s) 82, 1105
Bpu1102I GCTNAGC 1 cut(s) 265
Bpu14I TTCGAA 1 cut(s) 764
BpuMI CCSGG 1 cut(s) 491
BsaAI YACGTR 2 cut(s) 328, 682
BsaJI CCNNGG 7 cut(s) 14, 490, 559, 617, 991, 1161, 1212
BsaXI ACNNNNNCTCC 4 cut(s) 96, 126, 486, 516
Bsc4I CCNNNNNNNGG 8 cut(s) 26, 127, 244, 371, 448, 685, 790, 963
Bse118I RCCGGY 2 cut(s) 203, 512
Bse1I ACTGG 3 cut(s) 248, 535, 1088
Bse21I CCTNAGG 1 cut(s) 25
Bse3DI GCAATG 3 cut(s) 507, 694, 849
BseBI CCWGG 4 cut(s) 100, 372, 619, 1162
BseDI CCNNGG 7 cut(s) 14, 490, 559, 617, 991, 1161, 1212
BseGI GGATG 4 cut(s) 393, 438, 531, 1057
BseLI CCNNNNNNNGG 8 cut(s) 26, 127, 244, 371, 448, 685, 790, 963
BseMI GCAATG 3 cut(s) 507, 694, 849
BseMII CTCAG 2 cut(s) 39, 177
BseNI ACTGG 3 cut(s) 248, 535, 1088
BseRI GAGGAG 8 cut(s) 13, 41, 44, 47, 56, 65, 467, 622
BseX3I CGGCCG 1 cut(s) 487
BseXI GCAGC 1 cut(s) 994
BsgI GTGCAG 3 cut(s) 551, 990, 993
Bsh1285I CGRYCG 1 cut(s) 490
BshFI GGCC 5 cut(s) 19, 30, 103, 489, 520
BshNI GGYRCC 1 cut(s) 1136
BsiEI CGRYCG 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 614
BsiSI CCGG 6 cut(s) 204, 342, 490, 513, 1140, 1221
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 8 cut(s) 26, 127, 244, 371, 448, 685, 790, 963
BsmAI GTCTC 1 cut(s) 306
BsmBI CGTCTC 1 cut(s) 306
BsmFI GGGAC 1 cut(s) 214
BsmI GAATGC 1 cut(s) 894
BsnI GGCC 5 cut(s) 19, 30, 103, 489, 520
Bsp119I TTCGAA 1 cut(s) 764
Bsp1286I GDGCHC 2 cut(s) 614, 1247
Bsp143I GATC 4 cut(s) 414, 459, 663, 1022
Bsp1720I GCTNAGC 1 cut(s) 265
BspACI CCGC 3 cut(s) 198, 229, 516
BspANI GGCC 5 cut(s) 19, 30, 103, 489, 520
BspCNI CTCAG 2 cut(s) 38, 176
BspHI TCATGA 1 cut(s) 462
BspLI GGNNCC 6 cut(s) 31, 202, 376, 677, 745, 1138
BspPI GGATC 2 cut(s) 422, 1017
BspT104I TTCGAA 1 cut(s) 764
BspT107I GGYRCC 1 cut(s) 1136
BsrDI GCAATG 3 cut(s) 507, 694, 849
BsrFI RCCGGY 2 cut(s) 203, 512
BsrI ACTGG 3 cut(s) 248, 535, 1088
BssAI RCCGGY 2 cut(s) 203, 512
BssECI CCNNGG 7 cut(s) 14, 490, 559, 617, 991, 1161, 1212
BssMI GATC 4 cut(s) 414, 459, 663, 1022
BssNAI GTATAC 1 cut(s) 822
BssT1I CCWWGG 2 cut(s) 14, 991
Bst1107I GTATAC 1 cut(s) 822
Bst2UI CCWGG 4 cut(s) 100, 372, 619, 1162
Bst4CI ACNGT 2 cut(s) 640, 1115
Bst6I CTCTTC 1 cut(s) 304
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstBAI YACGTR 2 cut(s) 328, 682
BstBI TTCGAA 1 cut(s) 764
BstC8I GCNNGC 4 cut(s) 227, 514, 518, 584
BstDEI CTNAG 4 cut(s) 25, 163, 265, 1229
BstDSI CCRYGG 2 cut(s) 559, 1212
BstEII GGTNACC 1 cut(s) 1036
BstF5I GGATG 4 cut(s) 393, 438, 531, 1057
BstHHI GCGC 1 cut(s) 1226
BstKTI GATC 4 cut(s) 417, 462, 666, 1025
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 4 cut(s) 414, 459, 663, 1022
BstMCI CGRYCG 1 cut(s) 490
BstNI CCWGG 4 cut(s) 100, 372, 619, 1162
BstNSI RCATGY 1 cut(s) 841
BstPI GGTNACC 1 cut(s) 1036
BstSCI CCNGG 5 cut(s) 98, 370, 489, 617, 1160
BstV1I GCAGC 1 cut(s) 994
BstX2I RGATCY 1 cut(s) 1022
BstYI RGATCY 1 cut(s) 1022
BstZ17I GTATAC 1 cut(s) 822
BstZI CGGCCG 1 cut(s) 487
Bsu36I CCTNAGG 1 cut(s) 25
BsuRI GGCC 5 cut(s) 19, 30, 103, 489, 520
BtgI CCRYGG 2 cut(s) 559, 1212
BtgZI GCGATG 1 cut(s) 360
BtsCI GGATG 4 cut(s) 393, 438, 531, 1057
BtsI GCAGTG 1 cut(s) 747
BtsIMutI CAGTG 1 cut(s) 747
Cac8I GCNNGC 4 cut(s) 227, 514, 518, 584
CciI TCATGA 1 cut(s) 462
CfoI GCGC 1 cut(s) 1226
Cfr10I RCCGGY 2 cut(s) 203, 512
Cfr13I GGNCC 8 cut(s) 18, 29, 201, 518, 636, 691, 744, 880
CseI GACGC 2 cut(s) 486, 787
Csp6I GTAC 3 cut(s) 134, 143, 503
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviQI GTAC 3 cut(s) 134, 143, 503
DdeI CTNAG 4 cut(s) 25, 163, 265, 1229
DpnI GATC 4 cut(s) 416, 461, 665, 1024
DpnII GATC 4 cut(s) 414, 459, 663, 1022
DriI GACNNNNNGTC 2 cut(s) 389, 658
EaeI YGGCCR 1 cut(s) 487
EagI CGGCCG 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 304
Eam1105I GACNNNNNGTC 2 cut(s) 389, 658
EarI CTCTTC 1 cut(s) 304
Ecl136II GAGCTC 1 cut(s) 612
EclXI CGGCCG 1 cut(s) 487
Eco130I CCWWGG 2 cut(s) 14, 991
Eco24I GRGCYC 2 cut(s) 614, 1247
Eco32I GATATC 1 cut(s) 1018
Eco47I GGWCC 5 cut(s) 201, 636, 691, 744, 880
Eco52I CGGCCG 1 cut(s) 487
Eco53kI GAGCTC 1 cut(s) 612
Eco57I CTGAAG 1 cut(s) 467
Eco72I CACGTG 1 cut(s) 682
Eco81I CCTNAGG 1 cut(s) 25
Eco91I GGTNACC 1 cut(s) 1036
EcoICRI GAGCTC 1 cut(s) 612
EcoO109I RGGNCCY 2 cut(s) 18, 29
EcoO65I GGTNACC 1 cut(s) 1036
EcoRII CCWGG 4 cut(s) 98, 370, 617, 1160
EcoRV GATATC 1 cut(s) 1018
EcoT14I CCWWGG 2 cut(s) 14, 991
EcoT22I ATGCAT 1 cut(s) 527
EcoT38I GRGCYC 2 cut(s) 614, 1247
ErhI CCWWGG 2 cut(s) 14, 991
Esp3I CGTCTC 1 cut(s) 306
FaqI GGGAC 1 cut(s) 214
FauI CCCGC 2 cut(s) 191, 509
FbaI TGATCA 1 cut(s) 459
FblI GTMKAC 1 cut(s) 821
Fnu4HI GCNGC 1 cut(s) 1008
FokI GGATG 4 cut(s) 380, 425, 518, 1064
FriOI GRGCYC 2 cut(s) 614, 1247
Fsp4HI GCNGC 1 cut(s) 1008
FspBI CTAG 3 cut(s) 171, 1152, 1196
GlaI GCGC 1 cut(s) 1225
GluI GCNGC 1 cut(s) 1008
GsuI CTGGAG 2 cut(s) 82, 1105
HaeIII GGCC 5 cut(s) 19, 30, 103, 489, 520
HapII CCGG 6 cut(s) 204, 342, 490, 513, 1140, 1221
HgaI GACGC 2 cut(s) 486, 787
HhaI GCGC 1 cut(s) 1226
Hin6I GCGC 1 cut(s) 1224
HinP1I GCGC 1 cut(s) 1224
HinfI GANTC 3 cut(s) 161, 338, 815
HpaII CCGG 6 cut(s) 204, 342, 490, 513, 1140, 1221
HphI GGTGA 6 cut(s) 520, 616, 805, 838, 934, 1048
Hpy166II GTNNAC 6 cut(s) 361, 505, 691, 747, 822, 925
Hpy188I TCNGA 4 cut(s) 290, 646, 1022, 1093
Hpy188III TCNNGA 3 cut(s) 463, 475, 541
Hpy8I GTNNAC 6 cut(s) 361, 505, 691, 747, 822, 925
Hpy99I CGWCG 3 cut(s) 221, 488, 656
HpyAV CCTTC 4 cut(s) 442, 481, 866, 1088
HpyCH4III ACNGT 2 cut(s) 640, 1115
HpyCH4IV ACGT 2 cut(s) 327, 681
HpyF3I CTNAG 4 cut(s) 25, 163, 265, 1229
HpySE526I ACGT 2 cut(s) 327, 681
HspAI GCGC 1 cut(s) 1224
KroI GCCGGC 1 cut(s) 512
KroNI GCCGGC 1 cut(s) 514
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 4 cut(s) 414, 459, 663, 1022
LmnI GCTCC 6 cut(s) 454, 494, 609, 617, 675, 1142
Lsp1109I GCAGC 1 cut(s) 994
LweI GCATC 6 cut(s) 534, 721, 786, 1042, 1095, 1139
MaeI CTAG 3 cut(s) 171, 1152, 1196
MaeII ACGT 2 cut(s) 327, 681
MaeIII GTNAC 4 cut(s) 331, 428, 541, 1036
MalI GATC 4 cut(s) 416, 461, 665, 1024
MboI GATC 4 cut(s) 414, 459, 663, 1022
MboII GAAGA 6 cut(s) 262, 321, 331, 658, 1084, 1182
MflI RGATCY 1 cut(s) 1022
MhlI GDGCHC 2 cut(s) 614, 1247
MluCI AATT 4 cut(s) 125, 735, 769, 1075
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 2 cut(s) 732, 1071
Mph1103I ATGCAT 1 cut(s) 527
MroNI GCCGGC 1 cut(s) 512
MroXI GAANNNNTTC 1 cut(s) 470
MslI CAYNNNNRTG 1 cut(s) 1050
MspA1I CMGCKG 1 cut(s) 445
MspI CCGG 6 cut(s) 204, 342, 490, 513, 1140, 1221
MspR9I CCNGG 5 cut(s) 100, 372, 491, 619, 1162
Mva1269I GAATGC 1 cut(s) 894
MvaI CCWGG 4 cut(s) 100, 372, 619, 1162
NaeI GCCGGC 1 cut(s) 514
NciI CCSGG 1 cut(s) 491
NdeII GATC 4 cut(s) 414, 459, 663, 1022
NgoMIV GCCGGC 1 cut(s) 512
NlaIV GGNNCC 6 cut(s) 31, 202, 376, 677, 745, 1138
NmuCI GTSAC 4 cut(s) 331, 428, 541, 1036
NsiI ATGCAT 1 cut(s) 527
NspI RCATGY 1 cut(s) 841
NspV TTCGAA 1 cut(s) 764
PagI TCATGA 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 654
PctI GAATGC 1 cut(s) 894
PdiI GCCGGC 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 2 cut(s) 338, 815
PkrI GCNGC 1 cut(s) 1009
PleI GAGTC 1 cut(s) 155
PmaCI CACGTG 1 cut(s) 682
PmlI CACGTG 1 cut(s) 682
PpsI GAGTC 1 cut(s) 155
Ppu21I YACGTR 2 cut(s) 328, 682
Psp124BI GAGCTC 1 cut(s) 614
Psp6I CCWGG 4 cut(s) 98, 370, 617, 1160
PspCI CACGTG 1 cut(s) 682
PspEI GGTNACC 1 cut(s) 1036
PspGI CCWGG 4 cut(s) 98, 370, 617, 1160
PspN4I GGNNCC 6 cut(s) 31, 202, 376, 677, 745, 1138
PspPI GGNCC 8 cut(s) 18, 29, 201, 518, 636, 691, 744, 880
PsrI GAACNNNNNNTAC 4 cut(s) 170, 202, 917, 949
PsuI RGATCY 1 cut(s) 1022
PvuII CAGCTG 1 cut(s) 445
RsaI GTAC 3 cut(s) 135, 144, 504
RsaNI GTAC 3 cut(s) 134, 143, 503
RseI CAYNNNNRTG 1 cut(s) 1050
SacI GAGCTC 1 cut(s) 614
SatI GCNGC 1 cut(s) 1008
Sau3AI GATC 4 cut(s) 414, 459, 663, 1022
Sau96I GGNCC 8 cut(s) 18, 29, 201, 518, 636, 691, 744, 880
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 5 cut(s) 100, 372, 491, 619, 1162
SduI GDGCHC 2 cut(s) 614, 1247
SfaNI GCATC 6 cut(s) 534, 721, 786, 1042, 1095, 1139
SfuI TTCGAA 1 cut(s) 764
SinI GGWCC 5 cut(s) 201, 636, 691, 744, 880
SmiMI CAYNNNNRTG 1 cut(s) 1050
Sse9I AATT 4 cut(s) 125, 735, 769, 1075
SsiI CCGC 3 cut(s) 198, 229, 516
SspI AATATT 1 cut(s) 154
SspMI CTAG 3 cut(s) 171, 1152, 1196
SstI GAGCTC 1 cut(s) 614
StyD4I CCNGG 5 cut(s) 98, 370, 489, 617, 1160
StyI CCWWGG 2 cut(s) 14, 991
TaaI ACNGT 2 cut(s) 640, 1115
TaiI ACGT 2 cut(s) 330, 684
TaqI TCGA 5 cut(s) 216, 483, 662, 764, 1169
TasI AATT 4 cut(s) 125, 735, 769, 1075
TatI WGTACW 1 cut(s) 133
TfiI GAWTC 2 cut(s) 338, 815
TscAI CASTG 1 cut(s) 754
TseFI GTSAC 4 cut(s) 331, 428, 541, 1036
TseI GCWGC 1 cut(s) 1007
Tsp45I GTSAC 4 cut(s) 331, 428, 541, 1036
TspDTI ATGAA 2 cut(s) 98, 479
TspGWI ACGGA 2 cut(s) 490, 576
TspRI CASTG 1 cut(s) 754
VpaK11BI GGWCC 5 cut(s) 201, 636, 691, 744, 880
XceI RCATGY 1 cut(s) 841
XcmI CCANNNNNNNNNTGG 1 cut(s) 1065
XmiI GTMKAC 1 cut(s) 821
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 3 cut(s) 171, 1152, 1196
Zsp2I ATGCAT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.