MD14G1167100.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006447
Physical Location & Seq
Reverse (-)
26011735 .. 26013966
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1167100.v1.1.491

Sequence Viewer

Length: 1236 bp
ATGGCAAGGCCCTCCTCAGGCCCCTCACTTCTCTCTCTCCTCCTCTTCTCTCTCCTCTCCCCAACCCTCATTTCCTGCAGGCCACTTCATCTTCAAGACCCTGAATTGGTAGTACAAGAGGTACAAAGGAACATTAGCGACTCAGTGTCTAGGAGGAACTTGGGCTACTTATCATGTGGGACTGGAAACCCTATCGACGACTGCTGGCGGTGCGATCCGAACTGGGAGAAGAACAGGCAGCGTCTAGCAGATTGCGCGATAGGGTTCGGGAAAAACGCCATCGGTGGAAGAGACGGGAAGCTTTACGTGGTCACAGATTCCGGCGACGACGACGCCGTGAACCCCAAGCCAGGAACCCTACGACACGCCGTCATCCAAGACGAGCCGTTATGGATCATTTTCCAGCGCGACATGACCATCCAGCTCAAGGAGGAGCTGATCATGAACTCCTTCAAGACAATCGACGGCCGGGGAGCGTCCGTCCACATTGCCGGCGGGCCATGCATCACCATCCAGTTCGTGACCAACATTATTATCCACGGACTGAACATACACGATTGCAAGCAGGGAGGGAACGCTATGGTGAGGAACTCCCCAGAGCACTTCGGGTGGAGGACCATATCGGACGGCGACGGAGTGTCGATCTTCGGTGGGAGCCACGTGTGGGTGGACCATTGCTCGTTGTCCAACTGCAAAGATGGGTTGATTGATGCTATTCATGGGTCCACTGCGATAACAATTTCAAACAATTACATGACGCACCATGATAAGGTGATGCTTTTGGGACATAGTGATTCGTATACACAAGACAAGAACATGCAAGTCACCATTGCATTCAATCACTTTGGAGAAGGGTTGGTTCAAAGAATGCCAAGATGTAGGCATGGATATTTCCATGTGGTGAACAATGACTATACCCATTGGGAGATGTACGCTATTGGAGGGAGTGCAAACCCTACAATCAATAGCCAAGGGAACAGATTTGCCGCACCAGATATCAGATTCAGCAAAGAGGTGACCAAACATGAGGATGCACCAGAAAGTGAATGGAAGAACTGGAATTGGAGGTCGGAAGGAGACTTGATGTTAAACGGTGCGTTTTTTAGGGCATCGGGTGCCGGAGCTTCCTCAAGCTATGCCAGGGCTTCGAGCTTGGGTGCAAGGCCATCTTCCCTAGTGGGTTCAATCACCACGACTGCCGGCGCACTTAGCTGCCGAAAGGGCTCTCATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

412

Amino Acids

45.1

Weight (kDa)

6.64

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 150 - 341 2.5e-20 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1115
AccI GTMKAC 1 cut(s) 800
AccII CGCG 2 cut(s) 257, 408
AciI CCGC 3 cut(s) 208, 495, 987
AclWI GGATC 2 cut(s) 209, 401
AcoI YGGCCR 1 cut(s) 466
AcvI CACGTG 1 cut(s) 661
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 3 cut(s) 114, 123, 932
AfiI CCNNNNNNNGG 6 cut(s) 17, 106, 350, 427, 664, 769
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 6 cut(s) 95, 454, 744, 838, 863, 1185
AhdI GACNNNNNGTC 3 cut(s) 145, 368, 637
AjnI CCWGG 2 cut(s) 349, 1139
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 7 cut(s) 301, 424, 436, 1124, 1134, 1152, 1212
AluI AGCT 7 cut(s) 301, 424, 436, 1124, 1134, 1152, 1212
Alw21I GWGCWC 1 cut(s) 603
Alw26I GTCTC 2 cut(s) 285, 1071
AlwI GGATC 2 cut(s) 209, 401
AoxI GGCC 6 cut(s) 8, 19, 80, 466, 497, 1163
ApeKI GCWGC 2 cut(s) 238, 1212
Asp700I GAANNNNTTC 1 cut(s) 449
AspLEI GCGC 3 cut(s) 257, 408, 1205
AspS9I GGNCC 6 cut(s) 9, 20, 497, 615, 670, 723
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 7 cut(s) 499, 595, 784, 817, 913, 1027, 1180
AvaII GGWCC 3 cut(s) 615, 670, 723
AxyI CCTNAGG 1 cut(s) 16
BanI GGYRCC 1 cut(s) 1115
BanII GRGCYC 1 cut(s) 1226
BbrPI CACGTG 1 cut(s) 661
Bbv12I GWGCWC 1 cut(s) 603
BbvI GCAGC 2 cut(s) 250, 1199
BccI CCATC 5 cut(s) 287, 425, 518, 692, 1174
BceAI ACGGC 5 cut(s) 320, 353, 370, 481, 643
BciT130I CCWGG 2 cut(s) 351, 1141
BclI TGATCA 1 cut(s) 438
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 2 cut(s) 285, 1071
BfaI CTAG 3 cut(s) 150, 245, 1175
BfmI CTRYAG 1 cut(s) 76
BglI GCCNNNNNGGC 1 cut(s) 1221
BisI GCNGC 3 cut(s) 239, 987, 1213
BlsI GCNGC 3 cut(s) 240, 988, 1214
Bme1390I CCNGG 3 cut(s) 351, 470, 1141
Bme18I GGWCC 3 cut(s) 615, 670, 723
BmeRI GACNNNNNGTC 3 cut(s) 145, 368, 637
BmgT120I GGNCC 6 cut(s) 9, 20, 497, 615, 670, 723
BmiI GGNNCC 5 cut(s) 22, 355, 656, 724, 1117
BmrFI CCNGG 3 cut(s) 351, 470, 1141
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 5 cut(s) 513, 700, 765, 1021, 1118
BmuI ACTGGG 1 cut(s) 232
BpuEI CTTGAG 2 cut(s) 410, 1114
BpuMI CCSGG 1 cut(s) 470
BsaAI YACGTR 2 cut(s) 307, 661
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 4 cut(s) 469, 538, 970, 1140
BsaXI ACNNNNNCTCC 2 cut(s) 465, 495
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 106, 350, 427, 664, 769
Bse118I RCCGGY 2 cut(s) 491, 1199
Bse1I ACTGG 4 cut(s) 187, 227, 514, 1061
Bse21I CCTNAGG 1 cut(s) 16
Bse3DI GCAATG 4 cut(s) 486, 673, 828, 1228
BseBI CCWGG 2 cut(s) 351, 1141
BseDI CCNNGG 4 cut(s) 469, 538, 970, 1140
BseGI GGATG 4 cut(s) 372, 417, 510, 1036
BseLI CCNNNNNNNGG 6 cut(s) 17, 106, 350, 427, 664, 769
BseMI GCAATG 4 cut(s) 486, 673, 828, 1228
BseMII CTCAG 2 cut(s) 30, 156
BseNI ACTGG 4 cut(s) 187, 227, 514, 1061
BseRI GAGGAG 5 cut(s) 4, 29, 32, 44, 446
BseX3I CGGCCG 1 cut(s) 466
BseXI GCAGC 2 cut(s) 250, 1199
Bsh1236I CGCG 2 cut(s) 257, 408
Bsh1285I CGRYCG 1 cut(s) 469
BshFI GGCC 6 cut(s) 10, 21, 82, 468, 499, 1165
BshNI GGYRCC 1 cut(s) 1115
BsiEI CGRYCG 1 cut(s) 469
BsiHKAI GWGCWC 1 cut(s) 603
BsiSI CCGG 5 cut(s) 321, 469, 492, 1119, 1200
BslFI GGGAC 2 cut(s) 193, 798
BslI CCNNNNNNNGG 6 cut(s) 17, 106, 350, 427, 664, 769
BsmAI GTCTC 2 cut(s) 285, 1071
BsmBI CGTCTC 1 cut(s) 285
BsmFI GGGAC 2 cut(s) 193, 798
BsmI GAATGC 2 cut(s) 833, 873
BsnI GGCC 6 cut(s) 10, 21, 82, 468, 499, 1165
Bsp1286I GDGCHC 2 cut(s) 603, 1226
Bsp143I GATC 4 cut(s) 214, 393, 438, 642
BspACI CCGC 3 cut(s) 208, 495, 987
BspANI GGCC 6 cut(s) 10, 21, 82, 468, 499, 1165
BspCNI CTCAG 2 cut(s) 29, 155
BspFNI CGCG 2 cut(s) 257, 408
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 5 cut(s) 22, 355, 656, 724, 1117
BspMAI CTGCAG 1 cut(s) 80
BspPI GGATC 2 cut(s) 209, 401
BspT107I GGYRCC 1 cut(s) 1115
BsrDI GCAATG 4 cut(s) 486, 673, 828, 1228
BsrFI RCCGGY 2 cut(s) 491, 1199
BsrI ACTGG 4 cut(s) 187, 227, 514, 1061
BssAI RCCGGY 2 cut(s) 491, 1199
BssECI CCNNGG 4 cut(s) 469, 538, 970, 1140
BssMI GATC 4 cut(s) 214, 393, 438, 642
BssNAI GTATAC 1 cut(s) 801
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 970
Bst1107I GTATAC 1 cut(s) 801
Bst2UI CCWGG 2 cut(s) 351, 1141
Bst4CI ACNGT 1 cut(s) 1094
Bst6I CTCTTC 2 cut(s) 50, 283
BstACI GRCGYC 1 cut(s) 333
BstAPI GCANNNNNTGC 1 cut(s) 1115
BstBAI YACGTR 2 cut(s) 307, 661
BstC8I GCNNGC 6 cut(s) 80, 206, 493, 497, 563, 1201
BstDEI CTNAG 3 cut(s) 16, 142, 1208
BstDSI CCRYGG 1 cut(s) 538
BstEII GGTNACC 1 cut(s) 1015
BstF5I GGATG 4 cut(s) 372, 417, 510, 1036
BstFNI CGCG 2 cut(s) 257, 408
BstHHI GCGC 3 cut(s) 257, 408, 1205
BstKTI GATC 4 cut(s) 217, 396, 441, 645
BstMAI GTCTC 2 cut(s) 285, 1071
BstMBI GATC 4 cut(s) 214, 393, 438, 642
BstMCI CGRYCG 1 cut(s) 469
BstMWI GCNNNNNNNGC 7 cut(s) 210, 254, 501, 1115, 1209, 1221, 1230
BstNI CCWGG 2 cut(s) 351, 1141
BstNSI RCATGY 1 cut(s) 820
BstPI GGTNACC 1 cut(s) 1015
BstSCI CCNGG 3 cut(s) 349, 468, 1139
BstSFI CTRYAG 1 cut(s) 76
BstUI CGCG 2 cut(s) 257, 408
BstV1I GCAGC 2 cut(s) 250, 1199
BstZ17I GTATAC 1 cut(s) 801
BstZI CGGCCG 1 cut(s) 466
Bsu36I CCTNAGG 1 cut(s) 16
BsuRI GGCC 6 cut(s) 10, 21, 82, 468, 499, 1165
BtgI CCRYGG 1 cut(s) 538
BtsCI GGATG 4 cut(s) 372, 417, 510, 1036
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 2 cut(s) 150, 726
Cac8I GCNNGC 6 cut(s) 80, 206, 493, 497, 563, 1201
CciI TCATGA 1 cut(s) 441
CfoI GCGC 3 cut(s) 257, 408, 1205
Cfr10I RCCGGY 2 cut(s) 491, 1199
Cfr13I GGNCC 6 cut(s) 9, 20, 497, 615, 670, 723
CseI GACGC 4 cut(s) 230, 341, 465, 766
Csp6I GTAC 3 cut(s) 113, 122, 931
CviQI GTAC 3 cut(s) 113, 122, 931
DdeI CTNAG 3 cut(s) 16, 142, 1208
DpnI GATC 4 cut(s) 216, 395, 440, 644
DpnII GATC 4 cut(s) 214, 393, 438, 642
DriI GACNNNNNGTC 3 cut(s) 145, 368, 637
EaeI YGGCCR 1 cut(s) 466
EagI CGGCCG 1 cut(s) 466
Eam1104I CTCTTC 2 cut(s) 50, 283
Eam1105I GACNNNNNGTC 3 cut(s) 145, 368, 637
EarI CTCTTC 2 cut(s) 50, 283
EclXI CGGCCG 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 970
Eco24I GRGCYC 1 cut(s) 1226
Eco32I GATATC 1 cut(s) 997
Eco47I GGWCC 3 cut(s) 615, 670, 723
Eco52I CGGCCG 1 cut(s) 466
Eco72I CACGTG 1 cut(s) 661
Eco81I CCTNAGG 1 cut(s) 16
Eco91I GGTNACC 1 cut(s) 1015
EcoO109I RGGNCCY 2 cut(s) 9, 20
EcoO65I GGTNACC 1 cut(s) 1015
EcoRII CCWGG 2 cut(s) 349, 1139
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 1 cut(s) 970
EcoT22I ATGCAT 1 cut(s) 506
EcoT38I GRGCYC 1 cut(s) 1226
ErhI CCWWGG 1 cut(s) 970
Esp3I CGTCTC 1 cut(s) 285
FalI AAGNNNNNCTT 2 cut(s) 1153, 1185
FaqI GGGAC 2 cut(s) 193, 798
FauI CCCGC 1 cut(s) 488
FbaI TGATCA 1 cut(s) 438
FblI GTMKAC 1 cut(s) 800
Fnu4HI GCNGC 3 cut(s) 239, 987, 1213
FokI GGATG 4 cut(s) 359, 404, 497, 1043
FriOI GRGCYC 1 cut(s) 1226
Fsp4HI GCNGC 3 cut(s) 239, 987, 1213
FspBI CTAG 3 cut(s) 150, 245, 1175
GlaI GCGC 3 cut(s) 256, 407, 1204
GluI GCNGC 3 cut(s) 239, 987, 1213
HaeIII GGCC 6 cut(s) 10, 21, 82, 468, 499, 1165
HapII CCGG 5 cut(s) 321, 469, 492, 1119, 1200
HgaI GACGC 4 cut(s) 230, 341, 465, 766
HhaI GCGC 3 cut(s) 257, 408, 1205
Hin1I GRCGYC 1 cut(s) 333
Hin6I GCGC 3 cut(s) 255, 406, 1203
HinP1I GCGC 3 cut(s) 255, 406, 1203
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 4 cut(s) 140, 317, 794, 1002
HpaII CCGG 5 cut(s) 321, 469, 492, 1119, 1200
HphI GGTGA 7 cut(s) 499, 595, 784, 817, 913, 1027, 1180
Hpy166II GTNNAC 6 cut(s) 340, 484, 670, 726, 801, 904
Hpy188I TCNGA 4 cut(s) 219, 625, 1001, 1072
Hpy188III TCNNGA 5 cut(s) 95, 268, 442, 454, 520
Hpy8I GTNNAC 6 cut(s) 340, 484, 670, 726, 801, 904
Hpy99I CGWCG 6 cut(s) 200, 329, 332, 335, 467, 635
HpyAV CCTTC 3 cut(s) 460, 845, 1067
HpyCH4III ACNGT 1 cut(s) 1094
HpyCH4IV ACGT 2 cut(s) 306, 660
HpyCH4V TGCA 9 cut(s) 78, 504, 561, 693, 820, 833, 950, 1034, 1160
HpyF10VI GCNNNNNNNGC 7 cut(s) 210, 254, 501, 1115, 1209, 1221, 1230
HpyF3I CTNAG 3 cut(s) 16, 142, 1208
HpySE526I ACGT 2 cut(s) 306, 660
Hsp92I GRCGYC 1 cut(s) 333
HspAI GCGC 3 cut(s) 255, 406, 1203
KroI GCCGGC 2 cut(s) 491, 1199
KroNI GCCGGC 2 cut(s) 493, 1201
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 4 cut(s) 214, 393, 438, 642
LmnI GCTCC 4 cut(s) 433, 473, 654, 1121
Lsp1109I GCAGC 2 cut(s) 250, 1199
LweI GCATC 5 cut(s) 513, 700, 765, 1021, 1118
MaeI CTAG 3 cut(s) 150, 245, 1175
MaeII ACGT 2 cut(s) 306, 660
MaeIII GTNAC 4 cut(s) 310, 520, 823, 1015
MalI GATC 4 cut(s) 216, 395, 440, 644
MboI GATC 4 cut(s) 214, 393, 438, 642
MboII GAAGA 7 cut(s) 37, 83, 241, 300, 637, 1063, 1161
MhlI GDGCHC 2 cut(s) 603, 1226
MluCI AATT 4 cut(s) 104, 738, 748, 1060
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 2 cut(s) 711, 1050
Mph1103I ATGCAT 1 cut(s) 506
MroNI GCCGGC 2 cut(s) 491, 1199
MroXI GAANNNNTTC 1 cut(s) 449
MseI TTAA 1 cut(s) 1088
MslI CAYNNNNRTG 1 cut(s) 1029
MspI CCGG 5 cut(s) 321, 469, 492, 1119, 1200
MspR9I CCNGG 3 cut(s) 351, 470, 1141
Mva1269I GAATGC 2 cut(s) 833, 873
MvaI CCWGG 2 cut(s) 351, 1141
MvnI CGCG 2 cut(s) 257, 408
MwoI GCNNNNNNNGC 7 cut(s) 210, 254, 501, 1115, 1209, 1221, 1230
NaeI GCCGGC 2 cut(s) 493, 1201
NciI CCSGG 1 cut(s) 470
NdeII GATC 4 cut(s) 214, 393, 438, 642
NgoMIV GCCGGC 2 cut(s) 491, 1199
NlaIV GGNNCC 5 cut(s) 22, 355, 656, 724, 1117
NmuCI GTSAC 4 cut(s) 310, 520, 823, 1015
NsiI ATGCAT 1 cut(s) 506
NspI RCATGY 1 cut(s) 820
PagI TCATGA 1 cut(s) 441
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PctI GAATGC 2 cut(s) 833, 873
PdiI GCCGGC 2 cut(s) 493, 1201
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 3 cut(s) 317, 794, 1002
PkrI GCNGC 3 cut(s) 240, 988, 1214
PleI GAGTC 1 cut(s) 134
PmaCI CACGTG 1 cut(s) 661
PmlI CACGTG 1 cut(s) 661
PpsI GAGTC 1 cut(s) 134
Ppu21I YACGTR 2 cut(s) 307, 661
Psp6I CCWGG 2 cut(s) 349, 1139
PspCI CACGTG 1 cut(s) 661
PspEI GGTNACC 1 cut(s) 1015
PspGI CCWGG 2 cut(s) 349, 1139
PspN4I GGNNCC 5 cut(s) 22, 355, 656, 724, 1117
PspPI GGNCC 6 cut(s) 9, 20, 497, 615, 670, 723
PsrI GAACNNNNNNTAC 2 cut(s) 149, 181
PstI CTGCAG 1 cut(s) 80
RsaI GTAC 3 cut(s) 114, 123, 932
RsaNI GTAC 3 cut(s) 113, 122, 931
RseI CAYNNNNRTG 1 cut(s) 1029
SaqAI TTAA 1 cut(s) 1088
SatI GCNGC 3 cut(s) 239, 987, 1213
Sau3AI GATC 4 cut(s) 214, 393, 438, 642
Sau96I GGNCC 6 cut(s) 9, 20, 497, 615, 670, 723
SbfI CCTGCAGG 1 cut(s) 80
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 3 cut(s) 351, 470, 1141
SdaI CCTGCAGG 1 cut(s) 80
SduI GDGCHC 2 cut(s) 603, 1226
SfaNI GCATC 5 cut(s) 513, 700, 765, 1021, 1118
SfcI CTRYAG 1 cut(s) 76
SinI GGWCC 3 cut(s) 615, 670, 723
SmiMI CAYNNNNRTG 1 cut(s) 1029
SmlI CTYRAG 2 cut(s) 425, 1129
SmoI CTYRAG 2 cut(s) 425, 1129
Sse8387I CCTGCAGG 1 cut(s) 80
Sse9I AATT 4 cut(s) 104, 738, 748, 1060
SsiI CCGC 3 cut(s) 208, 495, 987
SspMI CTAG 3 cut(s) 150, 245, 1175
StyD4I CCNGG 3 cut(s) 349, 468, 1139
StyI CCWWGG 1 cut(s) 970
TaaI ACNGT 1 cut(s) 1094
TaiI ACGT 2 cut(s) 309, 663
TaqI TCGA 4 cut(s) 195, 462, 641, 1148
TasI AATT 4 cut(s) 104, 738, 748, 1060
TatI WGTACW 1 cut(s) 112
TauI GCSGC 1 cut(s) 989
TfiI GAWTC 3 cut(s) 317, 794, 1002
Tru1I TTAA 1 cut(s) 1088
Tru9I TTAA 1 cut(s) 1088
TscAI CASTG 2 cut(s) 150, 733
TseFI GTSAC 4 cut(s) 310, 520, 823, 1015
TseI GCWGC 2 cut(s) 238, 1212
Tsp45I GTSAC 4 cut(s) 310, 520, 823, 1015
TspDTI ATGAA 3 cut(s) 77, 458, 707
TspGWI ACGGA 3 cut(s) 469, 555, 648
TspRI CASTG 2 cut(s) 150, 733
VpaK11BI GGWCC 3 cut(s) 615, 670, 723
XceI RCATGY 1 cut(s) 820
XcmI CCANNNNNNNNNTGG 1 cut(s) 1044
XmiI GTMKAC 1 cut(s) 800
XmnI GAANNNNTTC 1 cut(s) 449
XspI CTAG 3 cut(s) 150, 245, 1175
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.