Rh7DG125100

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0006447
Physical Location & Seq
Reverse (-)
10568894 .. 10571137
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG125100.1

Sequence Viewer

Length: 879 bp
ATGACAATGCCTTCAGGCCCCTCTCTCTCTCTCCTCATCTTCCTCTTCTCTCTCCTCACCCTAACCCTCATTGTTTCCTCTGCACCAGTTCAAGATCCTGAACTCGTTGTACAGCAAGTACAAAGGAGCATCTATAATGCCTCAGTTACCCGGAGGAAGTTGGGCTATTTGTCATGTGGGTCCGGAAACCCGATCGACGACTGCTGGCGGTGCGACCCTAATTGGGAGAAGAACCGCCAGAGGCTCGCCGACTGCGCAATTGGGTTCGGCAAAAACGCCATTGGCGGCAGGGACGGGAGGATTTACGTGGTGACTGACTCAGGGGACGACGACCCCGTGAACCCCAAGCCAGGGACTCTACGACATGCCGTCATCCAGGACGAGCCCTTGTGGATCATTTTCGAGCGCGACATGTCGATCAAGCTCAAGGAGGAGCTGATCATGAACTCGTTCAAGACCCTGGACGGCCGAGGAGCCAGCGTGCACATCTCCGGCGGGCCATGCATTACGATCCAGTACGTGACCAACATTATCATACATGGACTCAACATACACGATTGCAAGCCGGGAGGGAATGCTATGGTGCGTGACTCCCCAAATCACTTCGGGTGGAGAACCATATCGGACGGTGATGGTGTGTCCATCTTCGGTGGGAGCCATGTTTGGGTGGACCATTGCTCCCTGTCGAACTGCGCCGACGGGCTGGTCGACGCAATTCATGGGTCCACTGCGATAACGATTTCGAACAATTACATGACGCACCATGATAAGGTCATGTTGCTGGGACACAGTGATACCTATACCCAGGACAAGAACATGCAGGTCACTATTGCCTTCAACCACTTTGGAGAAGGTCTTGTTCAAAGGATGCCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

292

Amino Acids

32.12

Weight (kDa)

6.15

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 208 - 292 2.8e-11 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 704
Acc16I TGCGCA 1 cut(s) 256
Acc36I ACCTGC 1 cut(s) 811
AccI GTMKAC 1 cut(s) 708
AccII CGCG 1 cut(s) 408
AccIII TCCGGA 1 cut(s) 182
AciI CCGC 4 cut(s) 208, 235, 285, 495
AclWI GGATC 3 cut(s) 89, 401, 505
AcoI YGGCCR 1 cut(s) 466
AfaI GTAC 3 cut(s) 111, 120, 518
AfiI CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 4 cut(s) 92, 454, 838, 863
AhdI GACNNNNNGTC 1 cut(s) 368
AjnI CCWGG 4 cut(s) 349, 375, 459, 804
AluBI AGCT 2 cut(s) 424, 436
AluI AGCT 2 cut(s) 424, 436
Alw21I GWGCWC 1 cut(s) 486
Alw44I GTGCAC 1 cut(s) 482
AlwI GGATC 3 cut(s) 89, 401, 505
Aor13HI TCCGGA 1 cut(s) 182
AoxI GGCC 3 cut(s) 16, 466, 497
ApaLI GTGCAC 1 cut(s) 482
Asp700I GAANNNNTTC 1 cut(s) 449
AspLEI GCGC 3 cut(s) 257, 408, 695
AspS9I GGNCC 5 cut(s) 17, 180, 497, 670, 723
AsuC2I CCSGG 2 cut(s) 151, 567
AsuHPI GGTGA 3 cut(s) 49, 322, 641
AsuII TTCGAA 1 cut(s) 743
AvaII GGWCC 3 cut(s) 180, 670, 723
BaeGI GKGCMC 1 cut(s) 486
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 486
BccI CCATC 2 cut(s) 626, 650
BceAI ACGGC 2 cut(s) 353, 481
BciT130I CCWGG 4 cut(s) 351, 377, 461, 806
BclI TGATCA 1 cut(s) 438
BcnI CCSGG 2 cut(s) 151, 567
BfuAI ACCTGC 1 cut(s) 811
BisI GCNGC 1 cut(s) 286
BlsI GCNGC 1 cut(s) 287
Bme1390I CCNGG 6 cut(s) 151, 351, 377, 461, 567, 806
Bme18I GGWCC 3 cut(s) 180, 670, 723
BmeRI GACNNNNNGTC 1 cut(s) 368
BmgT120I GGNCC 5 cut(s) 17, 180, 497, 670, 723
BmiI GGNNCC 5 cut(s) 19, 181, 475, 656, 724
BmrFI CCNGG 6 cut(s) 151, 351, 377, 461, 567, 806
BmsI GCATC 2 cut(s) 138, 858
Bpu14I TTCGAA 1 cut(s) 743
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 2 cut(s) 151, 567
BsaAI YACGTR 2 cut(s) 307, 520
BsaJI CCNNGG 5 cut(s) 350, 459, 469, 804, 872
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 4 cut(s) 465, 495, 662, 692
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
Bse1I ACTGG 2 cut(s) 86, 514
Bse3DI GCAATG 1 cut(s) 673
BseAI TCCGGA 1 cut(s) 182
BseBI CCWGG 4 cut(s) 351, 377, 461, 806
BseDI CCNNGG 5 cut(s) 350, 459, 469, 804, 872
BseGI GGATG 2 cut(s) 372, 873
BseLI CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 2 cut(s) 156, 333
BseNI ACTGG 2 cut(s) 86, 514
BseRI GAGGAG 4 cut(s) 23, 44, 446, 486
BseSI GKGCMC 1 cut(s) 486
BseX3I CGGCCG 1 cut(s) 466
BseYI CCCAGC 1 cut(s) 781
BsgI GTGCAG 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 408
Bsh1285I CGRYCG 2 cut(s) 195, 469
BshFI GGCC 3 cut(s) 18, 468, 499
BsiEI CGRYCG 2 cut(s) 195, 469
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 4 cut(s) 151, 183, 492, 566
BslFI GGGAC 4 cut(s) 305, 338, 367, 798
BslI CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
BsmFI GGGAC 4 cut(s) 305, 338, 367, 798
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 3 cut(s) 18, 468, 499
Bsp119I TTCGAA 1 cut(s) 743
Bsp1286I GDGCHC 2 cut(s) 387, 486
Bsp13I TCCGGA 1 cut(s) 182
Bsp1407I TGTACA 1 cut(s) 109
Bsp143I GATC 6 cut(s) 94, 192, 393, 417, 438, 510
BspACI CCGC 4 cut(s) 208, 235, 285, 495
BspANI GGCC 3 cut(s) 18, 468, 499
BspCNI CTCAG 2 cut(s) 155, 332
BspEI TCCGGA 1 cut(s) 182
BspFNI CGCG 1 cut(s) 408
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 5 cut(s) 19, 181, 475, 656, 724
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 3 cut(s) 89, 401, 505
BspT104I TTCGAA 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 673
BsrGI TGTACA 1 cut(s) 109
BsrI ACTGG 2 cut(s) 86, 514
BssECI CCNNGG 5 cut(s) 350, 459, 469, 804, 872
BssMI GATC 6 cut(s) 94, 192, 393, 417, 438, 510
BssT1I CCWWGG 1 cut(s) 872
Bst2UI CCWGG 4 cut(s) 351, 377, 461, 806
Bst4CI ACNGT 2 cut(s) 629, 791
Bst6I CTCTTC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 109
BstBAI YACGTR 2 cut(s) 307, 520
BstBI TTCGAA 1 cut(s) 743
BstC8I GCNNGC 6 cut(s) 206, 246, 478, 482, 497, 563
BstDEI CTNAG 2 cut(s) 142, 319
BstF5I GGATG 2 cut(s) 372, 873
BstFNI CGCG 1 cut(s) 408
BstHHI GCGC 3 cut(s) 257, 408, 695
BstKTI GATC 6 cut(s) 97, 195, 396, 420, 441, 513
BstMBI GATC 6 cut(s) 94, 192, 393, 417, 438, 510
BstMCI CGRYCG 2 cut(s) 195, 469
BstMWI GCNNNNNNNGC 3 cut(s) 210, 254, 501
BstNI CCWGG 4 cut(s) 351, 377, 461, 806
BstNSI RCATGY 3 cut(s) 368, 415, 820
BstSCI CCNGG 6 cut(s) 149, 349, 375, 459, 565, 804
BstSLI GKGCMC 1 cut(s) 486
BstUI CGCG 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BstZI CGGCCG 1 cut(s) 466
BsuRI GGCC 3 cut(s) 18, 468, 499
BtsCI GGATG 2 cut(s) 372, 873
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 2 cut(s) 726, 796
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 6 cut(s) 206, 246, 478, 482, 497, 563
CciI TCATGA 1 cut(s) 441
CfoI GCGC 3 cut(s) 257, 408, 695
Cfr13I GGNCC 5 cut(s) 17, 180, 497, 670, 723
CseI GACGC 2 cut(s) 719, 766
Csp6I GTAC 3 cut(s) 110, 119, 517
CviQI GTAC 3 cut(s) 110, 119, 517
DdeI CTNAG 2 cut(s) 142, 319
DpnI GATC 6 cut(s) 96, 194, 395, 419, 440, 512
DpnII GATC 6 cut(s) 94, 192, 393, 417, 438, 510
DrdI GACNNNNNNGTC 1 cut(s) 704
DriI GACNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 466
EagI CGGCCG 1 cut(s) 466
Eam1104I CTCTTC 1 cut(s) 50
Eam1105I GACNNNNNGTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 50
EclXI CGGCCG 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 872
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 3 cut(s) 180, 670, 723
Eco52I CGGCCG 1 cut(s) 466
EcoO109I RGGNCCY 1 cut(s) 17
EcoRII CCWGG 4 cut(s) 349, 375, 459, 804
EcoT14I CCWWGG 1 cut(s) 872
EcoT22I ATGCAT 1 cut(s) 506
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 872
FaqI GGGAC 4 cut(s) 305, 338, 367, 798
FauI CCCGC 1 cut(s) 488
FbaI TGATCA 1 cut(s) 438
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 1 cut(s) 286
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 1 cut(s) 286
FspI TGCGCA 1 cut(s) 256
GlaI GCGC 3 cut(s) 256, 407, 694
GluI GCNGC 1 cut(s) 286
GsaI CCCAGC 1 cut(s) 785
HaeIII GGCC 3 cut(s) 18, 468, 499
HapII CCGG 4 cut(s) 151, 183, 492, 566
HgaI GACGC 2 cut(s) 719, 766
HhaI GCGC 3 cut(s) 257, 408, 695
Hin6I GCGC 3 cut(s) 255, 406, 693
HinP1I GCGC 3 cut(s) 255, 406, 693
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HinfI GANTC 4 cut(s) 317, 355, 543, 590
HpaII CCGG 4 cut(s) 151, 183, 492, 566
HphI GGTGA 3 cut(s) 49, 322, 641
Hpy166II GTNNAC 5 cut(s) 340, 484, 670, 709, 726
Hpy188I TCNGA 1 cut(s) 625
Hpy188III TCNNGA 5 cut(s) 92, 98, 183, 442, 454
Hpy8I GTNNAC 5 cut(s) 340, 484, 670, 709, 726
Hpy99I CGWCG 4 cut(s) 200, 332, 701, 713
HpyAV CCTTC 3 cut(s) 21, 844, 845
HpyCH4III ACNGT 2 cut(s) 629, 791
HpyCH4IV ACGT 2 cut(s) 306, 519
HpyCH4V TGCA 5 cut(s) 83, 484, 504, 561, 820
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 254, 501
HpyF3I CTNAG 2 cut(s) 142, 319
HpySE526I ACGT 2 cut(s) 306, 519
HspAI GCGC 3 cut(s) 255, 406, 693
Kpn2I TCCGGA 1 cut(s) 182
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 6 cut(s) 94, 192, 393, 417, 438, 510
LmnI GCTCC 5 cut(s) 126, 433, 473, 654, 683
LweI GCATC 2 cut(s) 138, 858
MaeII ACGT 2 cut(s) 306, 519
MaeIII GTNAC 5 cut(s) 145, 310, 520, 587, 823
MalI GATC 6 cut(s) 96, 194, 395, 419, 440, 512
MboI GATC 6 cut(s) 94, 192, 393, 417, 438, 510
MboII GAAGA 4 cut(s) 31, 37, 241, 637
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 2 cut(s) 387, 486
MluCI AATT 4 cut(s) 220, 258, 714, 748
MlyI GAGTC 4 cut(s) 311, 349, 537, 584
Mph1103I ATGCAT 1 cut(s) 506
MroI TCCGGA 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 449
MspI CCGG 4 cut(s) 151, 183, 492, 566
MspR9I CCNGG 6 cut(s) 151, 351, 377, 461, 567, 806
MunI CAATTG 1 cut(s) 258
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 4 cut(s) 351, 377, 461, 806
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 3 cut(s) 210, 254, 501
NciI CCSGG 2 cut(s) 151, 567
NdeII GATC 6 cut(s) 94, 192, 393, 417, 438, 510
NlaIV GGNNCC 5 cut(s) 19, 181, 475, 656, 724
NmeAIII GCCGAG 1 cut(s) 494
NmuCI GTSAC 4 cut(s) 310, 520, 587, 823
NsbI TGCGCA 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 506
NspI RCATGY 3 cut(s) 368, 415, 820
NspV TTCGAA 1 cut(s) 743
PagI TCATGA 1 cut(s) 441
PciI ACATGT 1 cut(s) 411
PcsI WCGNNNNNNNCGW 2 cut(s) 333, 705
PctI GAATGC 1 cut(s) 580
PdmI GAANNNNTTC 1 cut(s) 449
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 1 cut(s) 287
Ple19I CGATCG 1 cut(s) 195
PleI GAGTC 4 cut(s) 311, 349, 537, 584
PpsI GAGTC 4 cut(s) 311, 349, 537, 584
Ppu21I YACGTR 2 cut(s) 307, 520
PscI ACATGT 1 cut(s) 411
Psp6I CCWGG 4 cut(s) 349, 375, 459, 804
PspFI CCCAGC 1 cut(s) 781
PspGI CCWGG 4 cut(s) 349, 375, 459, 804
PspN4I GGNNCC 5 cut(s) 19, 181, 475, 656, 724
PspPI GGNCC 5 cut(s) 17, 180, 497, 670, 723
PsrI GAACNNNNNNTAC 2 cut(s) 93, 125
PsuI RGATCY 1 cut(s) 94
PvuI CGATCG 1 cut(s) 195
RsaI GTAC 3 cut(s) 111, 120, 518
RsaNI GTAC 3 cut(s) 110, 119, 517
SalI GTCGAC 1 cut(s) 707
SatI GCNGC 1 cut(s) 286
Sau3AI GATC 6 cut(s) 94, 192, 393, 417, 438, 510
Sau96I GGNCC 5 cut(s) 17, 180, 497, 670, 723
SchI GAGTC 4 cut(s) 311, 349, 537, 584
ScrFI CCNGG 6 cut(s) 151, 351, 377, 461, 567, 806
SduI GDGCHC 2 cut(s) 387, 486
SetI ASST 9 cut(s) 309, 426, 438, 522, 774, 800, 825, 856, 878
SfaNI GCATC 2 cut(s) 138, 858
SfuI TTCGAA 1 cut(s) 743
SinI GGWCC 3 cut(s) 180, 670, 723
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 4 cut(s) 220, 258, 714, 748
SsiI CCGC 4 cut(s) 208, 235, 285, 495
StyD4I CCNGG 6 cut(s) 149, 349, 375, 459, 565, 804
StyI CCWWGG 1 cut(s) 872
TaaI ACNGT 2 cut(s) 629, 791
TaiI ACGT 2 cut(s) 309, 522
TaqI TCGA 6 cut(s) 195, 402, 416, 686, 708, 743
TasI AATT 4 cut(s) 220, 258, 714, 748
TatI WGTACW 2 cut(s) 109, 118
TauI GCSGC 1 cut(s) 288
TscAI CASTG 2 cut(s) 733, 796
TseFI GTSAC 4 cut(s) 310, 520, 587, 823
Tsp45I GTSAC 4 cut(s) 310, 520, 587, 823
TspDTI ATGAA 2 cut(s) 458, 707
TspRI CASTG 2 cut(s) 733, 796
VneI GTGCAC 1 cut(s) 482
VpaK11BI GGWCC 3 cut(s) 180, 670, 723
XceI RCATGY 3 cut(s) 368, 415, 820
XmiI GTMKAC 1 cut(s) 708
XmnI GAANNNNTTC 1 cut(s) 449
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.