RchiOBHm_Chr7g0191921

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006447
Physical Location & Seq
Reverse (-)
10556527 .. 10558982
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17149

Sequence Viewer

Length: 1236 bp
ATGACAATGCCTTCAGGCCCCTCTCTCTCTCTCCTCATCTTCCTCTTCTCTCTCCTCACCCTAGCCCTCATTGTTTCCTCTGCACCAGTTCAAGATCCTGAACTCGTTGTACAGCAAGTACAAAGGAGCATCTATAATGCCTCAGTTACCCGGAGGAAGTTGGGCTATTTGTCATGTGGGTCCGGAAACCCGATCGACGACTGCTGGCGGTGCGACCCTAATTGGGAGAAGAACCGCCAGAGGCTCGCCGACTGCGCAATTGGGTTCGGCAAAAACGCCATTGGCGGCAGGGACGGGAAGATTTACGTGGTGACTGACTCAGGGGACGACGACCCCGTGAACCCCAAGCCAGGGACTCTACGACATGCCGTCATCCAGGACGAGCCCTTGTGGATCATTTTCGAGCGCGACATGTCGATCAAGCTCAAGGAGGAGCTGATCATGAACTCGTTCAAGACCCTGGACGGCCGAGGAGCCAGCGTGCACATCTCCGGCGGGCCATGCATTACGATCCAGTACGTGACCAACATTATCATACATGGACTCAACATACACGATTGCAAGCCTGGAGGGAATGCTATGGTGCGTGACTCCCCAAATCACTTCGGGTGGAGAACCATATCGGACGGTGATGGTGTGTCCATCTTCGGTGGGAGCCATGTTTGGGTGGACCATTGCTCCCTGTCGAACTGCGCCGACGGGCTGGTCGACGCAATTCATGGGTCCACTGCGATAACGATTTCGAACAATTACATGACGCACCATGATAAGGTCATGTTGCTGGGACACAGTGATACCTATACCCAGGACAAGAACATGCAGGTCACTATTGCCTTCAACCACTTTGGAGAAGGTCTTGTTCAAAGGATGCCAAGATGCAGGCATGGATATTTCCATGTGGTGAACAATGACTACACCCATTGGGAGATGTACGCCATTGGAGGCAGTGCAAACCCTACTATCAATAGCCAGGGGAATAGGTTTACTGCACCAGATATAAGGTTCAGCAAAGAGGTGACAAAACATGAGGATGCACCTGAAAGTGAGTGGAAAAATTGGAACTGGAGGTCAGAGGGTGACATGTTGATAAACGGTGCATTTTTCACGGCCTCTGGTGCCGGAGCCTCTTCAAGCTATGCAAAAGCCTCAAGCTTGGGGGCAAGGCCATCTTCTCTTGTGGCTTCAATCACCACAACTTCTGGTGCACTCAATTGCCGAAAGGGTTCTCGTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

411

Amino Acids

45.0

Weight (kDa)

6.59

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 208 - 343 2e-20 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 704
Acc16I TGCGCA 1 cut(s) 256
Acc36I ACCTGC 1 cut(s) 811
AccB1I GGYRCC 1 cut(s) 1115
AccI GTMKAC 1 cut(s) 708
AccII CGCG 1 cut(s) 408
AccIII TCCGGA 1 cut(s) 182
AciI CCGC 4 cut(s) 208, 235, 285, 495
AclWI GGATC 3 cut(s) 89, 401, 505
AcoI YGGCCR 1 cut(s) 466
AfaI GTAC 4 cut(s) 111, 120, 518, 932
AfiI CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
AflIII ACRYGT 2 cut(s) 411, 1080
AgsI TTSAA 6 cut(s) 92, 454, 838, 863, 1131, 1185
AhdI GACNNNNNGTC 1 cut(s) 368
AjnI CCWGG 6 cut(s) 349, 375, 459, 565, 804, 969
AluBI AGCT 4 cut(s) 424, 436, 1134, 1152
AluI AGCT 4 cut(s) 424, 436, 1134, 1152
Alw21I GWGCWC 2 cut(s) 486, 1207
Alw44I GTGCAC 2 cut(s) 482, 1203
AlwI GGATC 3 cut(s) 89, 401, 505
Aor13HI TCCGGA 1 cut(s) 182
AoxI GGCC 5 cut(s) 16, 466, 497, 1107, 1163
ApaLI GTGCAC 2 cut(s) 482, 1203
Asp700I GAANNNNTTC 2 cut(s) 449, 1222
AspLEI GCGC 3 cut(s) 257, 408, 695
AspS9I GGNCC 5 cut(s) 17, 180, 497, 670, 723
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 7 cut(s) 49, 322, 641, 913, 1027, 1088, 1180
AsuII TTCGAA 1 cut(s) 743
AvaII GGWCC 3 cut(s) 180, 670, 723
BaeGI GKGCMC 2 cut(s) 486, 1207
BanI GGYRCC 1 cut(s) 1115
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 2 cut(s) 486, 1207
BccI CCATC 3 cut(s) 626, 650, 1174
BceAI ACGGC 3 cut(s) 353, 481, 1122
BciT130I CCWGG 6 cut(s) 351, 377, 461, 567, 806, 971
BclI TGATCA 1 cut(s) 438
BcnI CCSGG 1 cut(s) 151
BfaI CTAG 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 811
BisI GCNGC 1 cut(s) 286
BlsI GCNGC 1 cut(s) 287
Bme1390I CCNGG 7 cut(s) 151, 351, 377, 461, 567, 806, 971
Bme18I GGWCC 3 cut(s) 180, 670, 723
BmeRI GACNNNNNGTC 1 cut(s) 368
BmgT120I GGNCC 5 cut(s) 17, 180, 497, 670, 723
BmiI GGNNCC 7 cut(s) 19, 181, 475, 656, 724, 1117, 1123
BmrFI CCNGG 7 cut(s) 151, 351, 377, 461, 567, 806, 971
BmsI GCATC 4 cut(s) 138, 858, 866, 1021
BpmI CTGGAG 2 cut(s) 588, 1084
Bpu14I TTCGAA 1 cut(s) 743
BpuEI CTTGAG 2 cut(s) 410, 1132
BpuMI CCSGG 1 cut(s) 151
BsaAI YACGTR 2 cut(s) 307, 520
BsaJI CCNNGG 5 cut(s) 350, 459, 469, 804, 970
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 4 cut(s) 465, 495, 662, 692
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
Bse1I ACTGG 3 cut(s) 86, 514, 1067
Bse3DI GCAATG 1 cut(s) 673
BseAI TCCGGA 1 cut(s) 182
BseBI CCWGG 6 cut(s) 351, 377, 461, 567, 806, 971
BseDI CCNNGG 5 cut(s) 350, 459, 469, 804, 970
BseGI GGATG 3 cut(s) 372, 873, 1036
BseLI CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 2 cut(s) 156, 333
BseNI ACTGG 3 cut(s) 86, 514, 1067
BseRI GAGGAG 4 cut(s) 23, 44, 446, 486
BseSI GKGCMC 2 cut(s) 486, 1207
BseX3I CGGCCG 1 cut(s) 466
BseYI CCCAGC 1 cut(s) 781
BsgI GTGCAG 2 cut(s) 66, 972
Bsh1236I CGCG 1 cut(s) 408
Bsh1285I CGRYCG 2 cut(s) 195, 469
BshFI GGCC 5 cut(s) 18, 468, 499, 1109, 1165
BshNI GGYRCC 1 cut(s) 1115
BsiEI CGRYCG 2 cut(s) 195, 469
BsiHKAI GWGCWC 2 cut(s) 486, 1207
BsiSI CCGG 4 cut(s) 151, 183, 492, 1119
BslFI GGGAC 4 cut(s) 305, 338, 367, 798
BslI CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
BsmFI GGGAC 4 cut(s) 305, 338, 367, 798
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 5 cut(s) 18, 468, 499, 1109, 1165
Bsp119I TTCGAA 1 cut(s) 743
Bsp1286I GDGCHC 3 cut(s) 387, 486, 1207
Bsp13I TCCGGA 1 cut(s) 182
Bsp1407I TGTACA 1 cut(s) 109
Bsp143I GATC 6 cut(s) 94, 192, 393, 417, 438, 510
BspACI CCGC 4 cut(s) 208, 235, 285, 495
BspANI GGCC 5 cut(s) 18, 468, 499, 1109, 1165
BspCNI CTCAG 2 cut(s) 155, 332
BspEI TCCGGA 1 cut(s) 182
BspFNI CGCG 1 cut(s) 408
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 7 cut(s) 19, 181, 475, 656, 724, 1117, 1123
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 3 cut(s) 89, 401, 505
BspT104I TTCGAA 1 cut(s) 743
BspT107I GGYRCC 1 cut(s) 1115
BsrDI GCAATG 1 cut(s) 673
BsrGI TGTACA 1 cut(s) 109
BsrI ACTGG 3 cut(s) 86, 514, 1067
BssECI CCNNGG 5 cut(s) 350, 459, 469, 804, 970
BssMI GATC 6 cut(s) 94, 192, 393, 417, 438, 510
Bst2UI CCWGG 6 cut(s) 351, 377, 461, 567, 806, 971
Bst4CI ACNGT 3 cut(s) 629, 791, 1094
Bst6I CTCTTC 2 cut(s) 50, 1132
BstAUI TGTACA 1 cut(s) 109
BstBAI YACGTR 2 cut(s) 307, 520
BstBI TTCGAA 1 cut(s) 743
BstC8I GCNNGC 7 cut(s) 206, 246, 478, 482, 497, 563, 881
BstDEI CTNAG 2 cut(s) 142, 319
BstF5I GGATG 3 cut(s) 372, 873, 1036
BstFNI CGCG 1 cut(s) 408
BstHHI GCGC 3 cut(s) 257, 408, 695
BstKTI GATC 6 cut(s) 97, 195, 396, 420, 441, 513
BstMBI GATC 6 cut(s) 94, 192, 393, 417, 438, 510
BstMCI CGRYCG 2 cut(s) 195, 469
BstMWI GCNNNNNNNGC 4 cut(s) 210, 254, 501, 1115
BstNI CCWGG 6 cut(s) 351, 377, 461, 567, 806, 971
BstNSI RCATGY 4 cut(s) 368, 415, 820, 1084
BstSCI CCNGG 7 cut(s) 149, 349, 375, 459, 565, 804, 969
BstSLI GKGCMC 2 cut(s) 486, 1207
BstUI CGCG 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BstZI CGGCCG 1 cut(s) 466
BsuRI GGCC 5 cut(s) 18, 468, 499, 1109, 1165
BtsCI GGATG 3 cut(s) 372, 873, 1036
BtsI GCAGTG 2 cut(s) 726, 952
BtsIMutI CAGTG 3 cut(s) 726, 796, 952
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 7 cut(s) 206, 246, 478, 482, 497, 563, 881
CciI TCATGA 1 cut(s) 441
CfoI GCGC 3 cut(s) 257, 408, 695
Cfr13I GGNCC 5 cut(s) 17, 180, 497, 670, 723
CseI GACGC 2 cut(s) 719, 766
Csp6I GTAC 4 cut(s) 110, 119, 517, 931
CviQI GTAC 4 cut(s) 110, 119, 517, 931
DdeI CTNAG 2 cut(s) 142, 319
DpnI GATC 6 cut(s) 96, 194, 395, 419, 440, 512
DpnII GATC 6 cut(s) 94, 192, 393, 417, 438, 510
DrdI GACNNNNNNGTC 1 cut(s) 704
DriI GACNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 466
EagI CGGCCG 1 cut(s) 466
Eam1104I CTCTTC 2 cut(s) 50, 1132
Eam1105I GACNNNNNGTC 1 cut(s) 368
EarI CTCTTC 2 cut(s) 50, 1132
EclXI CGGCCG 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 3 cut(s) 180, 670, 723
Eco52I CGGCCG 1 cut(s) 466
EcoO109I RGGNCCY 1 cut(s) 17
EcoRII CCWGG 6 cut(s) 349, 375, 459, 565, 804, 969
EcoT22I ATGCAT 1 cut(s) 506
EcoT38I GRGCYC 1 cut(s) 387
FalI AAGNNNNNCTT 2 cut(s) 1153, 1185
FaqI GGGAC 4 cut(s) 305, 338, 367, 798
FauI CCCGC 1 cut(s) 488
FbaI TGATCA 1 cut(s) 438
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 1 cut(s) 286
FokI GGATG 3 cut(s) 359, 880, 1043
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 1 cut(s) 286
FspBI CTAG 1 cut(s) 62
FspI TGCGCA 1 cut(s) 256
GlaI GCGC 3 cut(s) 256, 407, 694
GluI GCNGC 1 cut(s) 286
GsaI CCCAGC 1 cut(s) 785
GsuI CTGGAG 2 cut(s) 588, 1084
HaeIII GGCC 5 cut(s) 18, 468, 499, 1109, 1165
HapII CCGG 4 cut(s) 151, 183, 492, 1119
HgaI GACGC 2 cut(s) 719, 766
HhaI GCGC 3 cut(s) 257, 408, 695
Hin6I GCGC 3 cut(s) 255, 406, 693
HinP1I GCGC 3 cut(s) 255, 406, 693
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HindIII AAGCTT 1 cut(s) 1150
HinfI GANTC 4 cut(s) 317, 355, 543, 590
HpaII CCGG 4 cut(s) 151, 183, 492, 1119
HphI GGTGA 7 cut(s) 49, 322, 641, 913, 1027, 1088, 1180
Hpy166II GTNNAC 8 cut(s) 340, 484, 670, 709, 726, 904, 984, 1205
Hpy188I TCNGA 2 cut(s) 625, 1072
Hpy188III TCNNGA 5 cut(s) 92, 98, 183, 442, 454
Hpy8I GTNNAC 8 cut(s) 340, 484, 670, 709, 726, 904, 984, 1205
Hpy99I CGWCG 4 cut(s) 200, 332, 701, 713
HpyAV CCTTC 3 cut(s) 21, 844, 845
HpyCH4III ACNGT 3 cut(s) 629, 791, 1094
HpyCH4IV ACGT 2 cut(s) 306, 519
HpyF10VI GCNNNNNNNGC 4 cut(s) 210, 254, 501, 1115
HpyF3I CTNAG 2 cut(s) 142, 319
HpySE526I ACGT 2 cut(s) 306, 519
HspAI GCGC 3 cut(s) 255, 406, 693
Kpn2I TCCGGA 1 cut(s) 182
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 6 cut(s) 94, 192, 393, 417, 438, 510
LmnI GCTCC 6 cut(s) 126, 433, 473, 654, 683, 1121
LweI GCATC 4 cut(s) 138, 858, 866, 1021
MaeI CTAG 1 cut(s) 62
MaeII ACGT 2 cut(s) 306, 519
MaeIII GTNAC 7 cut(s) 145, 310, 520, 587, 823, 1015, 1076
MalI GATC 6 cut(s) 96, 194, 395, 419, 440, 512
MboI GATC 6 cut(s) 94, 192, 393, 417, 438, 510
MboII GAAGA 7 cut(s) 31, 37, 241, 310, 637, 1119, 1161
MfeI CAATTG 2 cut(s) 258, 1210
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 3 cut(s) 387, 486, 1207
MluCI AATT 6 cut(s) 220, 258, 714, 748, 1054, 1210
MlyI GAGTC 4 cut(s) 311, 349, 537, 584
Mph1103I ATGCAT 1 cut(s) 506
MroI TCCGGA 1 cut(s) 182
MroXI GAANNNNTTC 2 cut(s) 449, 1222
MslI CAYNNNNRTG 1 cut(s) 1029
MspI CCGG 4 cut(s) 151, 183, 492, 1119
MspR9I CCNGG 7 cut(s) 151, 351, 377, 461, 567, 806, 971
MunI CAATTG 2 cut(s) 258, 1210
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 6 cut(s) 351, 377, 461, 567, 806, 971
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 4 cut(s) 210, 254, 501, 1115
NciI CCSGG 1 cut(s) 151
NdeII GATC 6 cut(s) 94, 192, 393, 417, 438, 510
NlaIV GGNNCC 7 cut(s) 19, 181, 475, 656, 724, 1117, 1123
NmeAIII GCCGAG 1 cut(s) 494
NmuCI GTSAC 6 cut(s) 310, 520, 587, 823, 1015, 1076
NsbI TGCGCA 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 506
NspI RCATGY 4 cut(s) 368, 415, 820, 1084
NspV TTCGAA 1 cut(s) 743
PagI TCATGA 1 cut(s) 441
PciI ACATGT 2 cut(s) 411, 1080
PcsI WCGNNNNNNNCGW 2 cut(s) 333, 705
PctI GAATGC 1 cut(s) 580
PdmI GAANNNNTTC 2 cut(s) 449, 1222
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 1 cut(s) 287
Ple19I CGATCG 1 cut(s) 195
PleI GAGTC 4 cut(s) 311, 349, 537, 584
PpsI GAGTC 4 cut(s) 311, 349, 537, 584
Ppu21I YACGTR 2 cut(s) 307, 520
PscI ACATGT 2 cut(s) 411, 1080
Psp6I CCWGG 6 cut(s) 349, 375, 459, 565, 804, 969
PspFI CCCAGC 1 cut(s) 781
PspGI CCWGG 6 cut(s) 349, 375, 459, 565, 804, 969
PspN4I GGNNCC 7 cut(s) 19, 181, 475, 656, 724, 1117, 1123
PspPI GGNCC 5 cut(s) 17, 180, 497, 670, 723
PsrI GAACNNNNNNTAC 4 cut(s) 93, 125, 896, 928
PsuI RGATCY 1 cut(s) 94
PvuI CGATCG 1 cut(s) 195
RsaI GTAC 4 cut(s) 111, 120, 518, 932
RsaNI GTAC 4 cut(s) 110, 119, 517, 931
RseI CAYNNNNRTG 1 cut(s) 1029
SalI GTCGAC 1 cut(s) 707
SatI GCNGC 1 cut(s) 286
Sau3AI GATC 6 cut(s) 94, 192, 393, 417, 438, 510
Sau96I GGNCC 5 cut(s) 17, 180, 497, 670, 723
SchI GAGTC 4 cut(s) 311, 349, 537, 584
ScrFI CCNGG 7 cut(s) 151, 351, 377, 461, 567, 806, 971
SduI GDGCHC 3 cut(s) 387, 486, 1207
SfaNI GCATC 4 cut(s) 138, 858, 866, 1021
SfuI TTCGAA 1 cut(s) 743
SinI GGWCC 3 cut(s) 180, 670, 723
SmiMI CAYNNNNRTG 1 cut(s) 1029
SmlI CTYRAG 2 cut(s) 425, 1147
SmoI CTYRAG 2 cut(s) 425, 1147
Sse9I AATT 6 cut(s) 220, 258, 714, 748, 1054, 1210
SsiI CCGC 4 cut(s) 208, 235, 285, 495
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 7 cut(s) 149, 349, 375, 459, 565, 804, 969
TaaI ACNGT 3 cut(s) 629, 791, 1094
TaiI ACGT 2 cut(s) 309, 522
TaqI TCGA 6 cut(s) 195, 402, 416, 686, 708, 743
TasI AATT 6 cut(s) 220, 258, 714, 748, 1054, 1210
TatI WGTACW 2 cut(s) 109, 118
TauI GCSGC 1 cut(s) 288
TscAI CASTG 3 cut(s) 733, 796, 952
TseFI GTSAC 6 cut(s) 310, 520, 587, 823, 1015, 1076
Tsp45I GTSAC 6 cut(s) 310, 520, 587, 823, 1015, 1076
TspDTI ATGAA 2 cut(s) 458, 707
TspRI CASTG 3 cut(s) 733, 796, 952
VneI GTGCAC 2 cut(s) 482, 1203
VpaK11BI GGWCC 3 cut(s) 180, 670, 723
XceI RCATGY 4 cut(s) 368, 415, 820, 1084
XmiI GTMKAC 1 cut(s) 708
XmnI GAANNNNTTC 2 cut(s) 449, 1222
XspI CTAG 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.