RLG00000004418

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006447
Physical Location & Seq
Forward (+)
59201850 .. 59203682
1833 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004418

Sequence Viewer

Length: 1236 bp
ATGGCAATGCCTTCAGGCCCCTCTCTCTCTCTCCTCATCTTCCTCTTCTCTCTCCTCACCCTAACCCTCATTGTTTCCTCTGCACCAGTTCAAGATCCTGAACTCGTTGTACAGCAAGTACAAAGGAGCATCTATAATGCCTCAGCTACCCGGAGGAAGTTGGGCTATTTGTCATGTGGGTCCGGAAACCCGATCGACGACTGCTGGCGGTGCGACCCGAATTGGGAGAAGAACCGCCAGAGGCTCGCCGACTGCGCAATTGGGTTCGGCAAAAATGCAATTGGCGGCAGGGACGGGAGGATTTACGTGGTCACTGACTCAGGGGACGACGACCCCGTGAACCCCAAGCCAGGGACTCTGCGACATGCCGTCATCCAGGACGAGCCCTTGTGGATCATTTTCGAGCGCGACATGTCGATCAAGCTCAAGGAGGAGCTGATCATGAACTCGTTCAAGACCCTCGACGGCCGAGGAGCCAGCGTGCACATCTCCGGCGGGCCATGCATTACGATCCAGTACGTGACCAACATTATCATACATGGACTCAACATACACGATTGCAAGCCGGGAGGGAATGCTATGGTGCGTGACTCCCCAAGTCACTTCGGGTGGAGAACCATATCGGACGGCGATGGCGTGTCCATCTTCGGTGGGAGCCATGTTTGGGTGGACCATAACTCCCTGTCGAACTGCGCCGACGGGCTGGTCGACGCAATTCATGGGTCCACTGCGATAACGATCTCGAACAATTACATGACGCACCATGATAAGGTCATGTTGCTGGGACACAGTGATACCTATACCCAGGACAAGAACATGCAGGTCACTATTGCCTTCAACCACTTTGGAGAAGGTCTTGTTCAAAGGATGCCAAGATGTAGGCATGGATATTTCCATGTGGTGAACAATGACTACACCCATTGGGAGATGTACGCCATTGGAGGCAGTGCAAACCCTACTATCAATAGCCAGGGGAATAGGTTTACTGCACCAGATATAAGGTTCAGCAAAGAGGTGACAAAACATGAGGATGCACCTGAAAGTGAGTGGAAGAATTGGAACTGGAGGTCAGAGGGTGACATGTTGATAAATGGTGCATTTTTCACGGCCTCTGGTGCCGGAGCTTCTTCAAGCTATGCAAAAGCTTCAAGCTTGGGAGCAAGGCCATCTTCTCTTGTGGCTTCAATCACCACAACTTCTGGTGCACTCAATTGCCGAAAGGGTTCTCGTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

412

Amino Acids

44.98

Weight (kDa)

6.59

Isoelectric Point (pI)

33.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 208 - 343 6e-20 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 704
Acc16I TGCGCA 1 cut(s) 256
Acc36I ACCTGC 1 cut(s) 811
AccB1I GGYRCC 1 cut(s) 1115
AccI GTMKAC 1 cut(s) 708
AccII CGCG 1 cut(s) 408
AccIII TCCGGA 1 cut(s) 182
AciI CCGC 4 cut(s) 208, 235, 285, 495
AclWI GGATC 3 cut(s) 89, 401, 505
AcoI YGGCCR 1 cut(s) 466
AfaI GTAC 4 cut(s) 111, 120, 518, 932
AfiI CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
AflIII ACRYGT 2 cut(s) 411, 1080
AgsI TTSAA 7 cut(s) 92, 454, 838, 863, 1131, 1149, 1185
AhdI GACNNNNNGTC 1 cut(s) 368
AjnI CCWGG 4 cut(s) 349, 375, 804, 969
AluBI AGCT 7 cut(s) 146, 424, 436, 1124, 1134, 1145, 1152
AluI AGCT 7 cut(s) 146, 424, 436, 1124, 1134, 1145, 1152
Alw21I GWGCWC 2 cut(s) 486, 1207
Alw44I GTGCAC 2 cut(s) 482, 1203
AlwI GGATC 3 cut(s) 89, 401, 505
Aor13HI TCCGGA 1 cut(s) 182
AoxI GGCC 5 cut(s) 16, 466, 497, 1107, 1163
ApaLI GTGCAC 2 cut(s) 482, 1203
Asp700I GAANNNNTTC 2 cut(s) 449, 1222
AspLEI GCGC 3 cut(s) 257, 408, 695
AspS9I GGNCC 5 cut(s) 17, 180, 497, 670, 723
AsuC2I CCSGG 2 cut(s) 151, 567
AsuHPI GGTGA 5 cut(s) 49, 913, 1027, 1088, 1180
AvaII GGWCC 3 cut(s) 180, 670, 723
BaeGI GKGCMC 2 cut(s) 486, 1207
BanI GGYRCC 1 cut(s) 1115
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 2 cut(s) 486, 1207
BbvCI CCTCAGC 1 cut(s) 142
BccI CCATC 3 cut(s) 626, 650, 1174
BceAI ACGGC 4 cut(s) 353, 481, 643, 1122
BciT130I CCWGG 4 cut(s) 351, 377, 806, 971
BclI TGATCA 1 cut(s) 438
BcnI CCSGG 2 cut(s) 151, 567
BfuAI ACCTGC 1 cut(s) 811
BisI GCNGC 1 cut(s) 286
BlsI GCNGC 1 cut(s) 287
Bme1390I CCNGG 6 cut(s) 151, 351, 377, 567, 806, 971
Bme18I GGWCC 3 cut(s) 180, 670, 723
BmeRI GACNNNNNGTC 1 cut(s) 368
BmgT120I GGNCC 5 cut(s) 17, 180, 497, 670, 723
BmiI GGNNCC 6 cut(s) 19, 181, 475, 656, 724, 1117
BmrFI CCNGG 6 cut(s) 151, 351, 377, 567, 806, 971
BmsI GCATC 3 cut(s) 138, 858, 1021
BpmI CTGGAG 1 cut(s) 1084
Bpu10I CCTNAGC 1 cut(s) 142
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 2 cut(s) 151, 567
BsaAI YACGTR 2 cut(s) 307, 520
BsaBI GATNNNNATC 1 cut(s) 737
BsaJI CCNNGG 4 cut(s) 350, 469, 804, 970
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 4 cut(s) 465, 495, 662, 692
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
Bse1I ACTGG 3 cut(s) 86, 514, 1067
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 737
BseAI TCCGGA 1 cut(s) 182
BseBI CCWGG 4 cut(s) 351, 377, 806, 971
BseDI CCNNGG 4 cut(s) 350, 469, 804, 970
BseGI GGATG 3 cut(s) 372, 873, 1036
BseJI GATNNNNATC 1 cut(s) 737
BseLI CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 156, 333
BseNI ACTGG 3 cut(s) 86, 514, 1067
BseRI GAGGAG 4 cut(s) 23, 44, 446, 486
BseSI GKGCMC 2 cut(s) 486, 1207
BseX3I CGGCCG 1 cut(s) 466
BseYI CCCAGC 1 cut(s) 781
BsgI GTGCAG 2 cut(s) 66, 972
Bsh1236I CGCG 1 cut(s) 408
Bsh1285I CGRYCG 2 cut(s) 195, 469
BshFI GGCC 5 cut(s) 18, 468, 499, 1109, 1165
BshNI GGYRCC 1 cut(s) 1115
BsiEI CGRYCG 2 cut(s) 195, 469
BsiHKAI GWGCWC 2 cut(s) 486, 1207
BsiSI CCGG 5 cut(s) 151, 183, 492, 566, 1119
BslFI GGGAC 4 cut(s) 305, 338, 367, 798
BslI CCNNNNNNNGG 5 cut(s) 223, 350, 351, 664, 769
BsmFI GGGAC 4 cut(s) 305, 338, 367, 798
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 5 cut(s) 18, 468, 499, 1109, 1165
Bsp1286I GDGCHC 3 cut(s) 387, 486, 1207
Bsp13I TCCGGA 1 cut(s) 182
Bsp1407I TGTACA 1 cut(s) 109
Bsp143I GATC 7 cut(s) 94, 192, 393, 417, 438, 510, 738
BspACI CCGC 4 cut(s) 208, 235, 285, 495
BspANI GGCC 5 cut(s) 18, 468, 499, 1109, 1165
BspCNI CTCAG 2 cut(s) 155, 332
BspEI TCCGGA 1 cut(s) 182
BspFNI CGCG 1 cut(s) 408
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 6 cut(s) 19, 181, 475, 656, 724, 1117
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 3 cut(s) 89, 401, 505
BspT107I GGYRCC 1 cut(s) 1115
BsrDI GCAATG 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 109
BsrI ACTGG 3 cut(s) 86, 514, 1067
BssECI CCNNGG 4 cut(s) 350, 469, 804, 970
BssMI GATC 7 cut(s) 94, 192, 393, 417, 438, 510, 738
Bst2UI CCWGG 4 cut(s) 351, 377, 806, 971
Bst4CI ACNGT 1 cut(s) 791
Bst6I CTCTTC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 109
BstBAI YACGTR 2 cut(s) 307, 520
BstC8I GCNNGC 6 cut(s) 206, 246, 478, 482, 497, 563
BstDEI CTNAG 2 cut(s) 142, 319
BstF5I GGATG 3 cut(s) 372, 873, 1036
BstFNI CGCG 1 cut(s) 408
BstHHI GCGC 3 cut(s) 257, 408, 695
BstKTI GATC 7 cut(s) 97, 195, 396, 420, 441, 513, 741
BstMBI GATC 7 cut(s) 94, 192, 393, 417, 438, 510, 738
BstMCI CGRYCG 2 cut(s) 195, 469
BstMWI GCNNNNNNNGC 4 cut(s) 210, 254, 501, 1115
BstNI CCWGG 4 cut(s) 351, 377, 806, 971
BstNSI RCATGY 4 cut(s) 368, 415, 820, 1084
BstSCI CCNGG 6 cut(s) 149, 349, 375, 565, 804, 969
BstSLI GKGCMC 2 cut(s) 486, 1207
BstUI CGCG 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BstZI CGGCCG 1 cut(s) 466
BsuRI GGCC 5 cut(s) 18, 468, 499, 1109, 1165
BtgZI GCGATG 1 cut(s) 645
BtsCI GGATG 3 cut(s) 372, 873, 1036
BtsI GCAGTG 2 cut(s) 726, 952
BtsIMutI CAGTG 4 cut(s) 312, 726, 796, 952
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 6 cut(s) 206, 246, 478, 482, 497, 563
CciI TCATGA 1 cut(s) 441
CfoI GCGC 3 cut(s) 257, 408, 695
Cfr13I GGNCC 5 cut(s) 17, 180, 497, 670, 723
CseI GACGC 2 cut(s) 719, 766
Csp6I GTAC 4 cut(s) 110, 119, 517, 931
CviQI GTAC 4 cut(s) 110, 119, 517, 931
DdeI CTNAG 2 cut(s) 142, 319
DpnI GATC 7 cut(s) 96, 194, 395, 419, 440, 512, 740
DpnII GATC 7 cut(s) 94, 192, 393, 417, 438, 510, 738
DrdI GACNNNNNNGTC 1 cut(s) 704
DriI GACNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 466
EagI CGGCCG 1 cut(s) 466
Eam1104I CTCTTC 1 cut(s) 50
Eam1105I GACNNNNNGTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 50
EclXI CGGCCG 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 3 cut(s) 180, 670, 723
Eco52I CGGCCG 1 cut(s) 466
EcoO109I RGGNCCY 1 cut(s) 17
EcoRII CCWGG 4 cut(s) 349, 375, 804, 969
EcoT22I ATGCAT 1 cut(s) 506
EcoT38I GRGCYC 1 cut(s) 387
FalI AAGNNNNNCTT 2 cut(s) 1153, 1185
FaqI GGGAC 4 cut(s) 305, 338, 367, 798
FauI CCCGC 1 cut(s) 488
FbaI TGATCA 1 cut(s) 438
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 1 cut(s) 286
FokI GGATG 3 cut(s) 359, 880, 1043
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 1 cut(s) 286
FspI TGCGCA 1 cut(s) 256
GlaI GCGC 3 cut(s) 256, 407, 694
GluI GCNGC 1 cut(s) 286
GsaI CCCAGC 1 cut(s) 785
GsuI CTGGAG 1 cut(s) 1084
HaeIII GGCC 5 cut(s) 18, 468, 499, 1109, 1165
HapII CCGG 5 cut(s) 151, 183, 492, 566, 1119
HgaI GACGC 2 cut(s) 719, 766
HhaI GCGC 3 cut(s) 257, 408, 695
Hin6I GCGC 3 cut(s) 255, 406, 693
HinP1I GCGC 3 cut(s) 255, 406, 693
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HindIII AAGCTT 2 cut(s) 1143, 1150
HinfI GANTC 4 cut(s) 317, 355, 543, 590
HpaII CCGG 5 cut(s) 151, 183, 492, 566, 1119
HphI GGTGA 5 cut(s) 49, 913, 1027, 1088, 1180
Hpy166II GTNNAC 8 cut(s) 340, 484, 670, 709, 726, 904, 984, 1205
Hpy188I TCNGA 2 cut(s) 625, 1072
Hpy188III TCNNGA 6 cut(s) 92, 98, 183, 442, 454, 742
Hpy8I GTNNAC 8 cut(s) 340, 484, 670, 709, 726, 904, 984, 1205
Hpy99I CGWCG 5 cut(s) 200, 332, 467, 701, 713
HpyAV CCTTC 3 cut(s) 21, 844, 845
HpyCH4III ACNGT 1 cut(s) 791
HpyCH4IV ACGT 2 cut(s) 306, 519
HpyF10VI GCNNNNNNNGC 4 cut(s) 210, 254, 501, 1115
HpyF3I CTNAG 2 cut(s) 142, 319
HpySE526I ACGT 2 cut(s) 306, 519
HspAI GCGC 3 cut(s) 255, 406, 693
Kpn2I TCCGGA 1 cut(s) 182
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 7 cut(s) 94, 192, 393, 417, 438, 510, 738
LmnI GCTCC 6 cut(s) 126, 433, 473, 654, 1121, 1157
LweI GCATC 3 cut(s) 138, 858, 1021
MaeII ACGT 2 cut(s) 306, 519
MaeIII GTNAC 7 cut(s) 310, 520, 587, 599, 823, 1015, 1076
MalI GATC 7 cut(s) 96, 194, 395, 419, 440, 512, 740
MboI GATC 7 cut(s) 94, 192, 393, 417, 438, 510, 738
MboII GAAGA 7 cut(s) 31, 37, 241, 637, 1063, 1119, 1161
MfeI CAATTG 3 cut(s) 258, 279, 1210
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 3 cut(s) 387, 486, 1207
MluCI AATT 7 cut(s) 220, 258, 279, 714, 748, 1054, 1210
MlyI GAGTC 4 cut(s) 311, 349, 537, 584
Mph1103I ATGCAT 1 cut(s) 506
MroI TCCGGA 1 cut(s) 182
MroXI GAANNNNTTC 2 cut(s) 449, 1222
MslI CAYNNNNRTG 1 cut(s) 1029
MspI CCGG 5 cut(s) 151, 183, 492, 566, 1119
MspR9I CCNGG 6 cut(s) 151, 351, 377, 567, 806, 971
MunI CAATTG 3 cut(s) 258, 279, 1210
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 4 cut(s) 351, 377, 806, 971
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 4 cut(s) 210, 254, 501, 1115
NciI CCSGG 2 cut(s) 151, 567
NdeII GATC 7 cut(s) 94, 192, 393, 417, 438, 510, 738
NlaIV GGNNCC 6 cut(s) 19, 181, 475, 656, 724, 1117
NmeAIII GCCGAG 1 cut(s) 494
NmuCI GTSAC 7 cut(s) 310, 520, 587, 599, 823, 1015, 1076
NsbI TGCGCA 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 506
NspI RCATGY 4 cut(s) 368, 415, 820, 1084
PagI TCATGA 1 cut(s) 441
PciI ACATGT 2 cut(s) 411, 1080
PcsI WCGNNNNNNNCGW 3 cut(s) 333, 633, 705
PctI GAATGC 1 cut(s) 580
PdmI GAANNNNTTC 2 cut(s) 449, 1222
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 1 cut(s) 287
Ple19I CGATCG 1 cut(s) 195
PleI GAGTC 4 cut(s) 311, 349, 537, 584
PpsI GAGTC 4 cut(s) 311, 349, 537, 584
Ppu21I YACGTR 2 cut(s) 307, 520
PscI ACATGT 2 cut(s) 411, 1080
Psp6I CCWGG 4 cut(s) 349, 375, 804, 969
PspFI CCCAGC 1 cut(s) 781
PspGI CCWGG 4 cut(s) 349, 375, 804, 969
PspN4I GGNNCC 6 cut(s) 19, 181, 475, 656, 724, 1117
PspPI GGNCC 5 cut(s) 17, 180, 497, 670, 723
PsrI GAACNNNNNNTAC 4 cut(s) 93, 125, 896, 928
PsuI RGATCY 1 cut(s) 94
PvuI CGATCG 1 cut(s) 195
RsaI GTAC 4 cut(s) 111, 120, 518, 932
RsaNI GTAC 4 cut(s) 110, 119, 517, 931
RseI CAYNNNNRTG 1 cut(s) 1029
SalI GTCGAC 1 cut(s) 707
SatI GCNGC 1 cut(s) 286
Sau3AI GATC 7 cut(s) 94, 192, 393, 417, 438, 510, 738
Sau96I GGNCC 5 cut(s) 17, 180, 497, 670, 723
SchI GAGTC 4 cut(s) 311, 349, 537, 584
ScrFI CCNGG 6 cut(s) 151, 351, 377, 567, 806, 971
SduI GDGCHC 3 cut(s) 387, 486, 1207
SfaNI GCATC 3 cut(s) 138, 858, 1021
SinI GGWCC 3 cut(s) 180, 670, 723
SmiMI CAYNNNNRTG 1 cut(s) 1029
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 7 cut(s) 220, 258, 279, 714, 748, 1054, 1210
SsiI CCGC 4 cut(s) 208, 235, 285, 495
StyD4I CCNGG 6 cut(s) 149, 349, 375, 565, 804, 969
TaaI ACNGT 1 cut(s) 791
TaiI ACGT 2 cut(s) 309, 522
TaqI TCGA 7 cut(s) 195, 402, 416, 462, 686, 708, 743
TasI AATT 7 cut(s) 220, 258, 279, 714, 748, 1054, 1210
TatI WGTACW 2 cut(s) 109, 118
TauI GCSGC 1 cut(s) 288
TscAI CASTG 4 cut(s) 319, 733, 796, 952
TseFI GTSAC 7 cut(s) 310, 520, 587, 599, 823, 1015, 1076
Tsp45I GTSAC 7 cut(s) 310, 520, 587, 599, 823, 1015, 1076
TspDTI ATGAA 2 cut(s) 458, 707
TspRI CASTG 4 cut(s) 319, 733, 796, 952
VneI GTGCAC 2 cut(s) 482, 1203
VpaK11BI GGWCC 3 cut(s) 180, 670, 723
XceI RCATGY 4 cut(s) 368, 415, 820, 1084
XmiI GTMKAC 1 cut(s) 708
XmnI GAANNNNTTC 2 cut(s) 449, 1222
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.