FvH4_5g07900

Preprotein translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015468
Physical Location & Seq
Reverse (-)
4589393 .. 4591981
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07900.t1

Sequence Viewer

Length: 843 bp
ATGGCGCTTCGCTTTCTCGCCCAGGTCCCGGTGATGTCACCCTTCCCGTCATCTTCAAAATCCGGCAACCTAACCTCCTCCGTCACACTGCAACCTCCACGTCTCATTCACCTGAAGATCAAGATCAAGAGAAAGCCGCCGCTCCTCACTTCAATCAAAGCCGTCGACGAAAGCCAAGCAAACTCTGACACCACCGAGGCAAAAGAAGAAGAGCCGTCGCCGACGGCGAGTGGATCCGGCGATTTGGGGTCGGAGATAAAGAAGGCGATGGAGGAGAGGAAGGAGAAGGAGAAAGAAGGAGGCGGCGGCGATTTGTTTAGCGGAGTGGCGGAGGAGGTTAGGGAGATAGAGTGGCCTGTGTTTGGTAAGGTCTTGGGGACTACCGGAGTTGTGCTTGGGGTTATTGCTGGCTCCAGTGTCGTTTTGCTCACTGTTAATGCCGTTTTGGCCGAGCTTTCCGACCGGGTTTTCGCCGGCAGAGATGCAAATGGAGTCTTGATGGGTCCGCAGGCTAAAGGAAGCGTATACATCTTTTCATATCCACAAGGTCTTCAGAAGCTTTTGAAGTTCATGAAGCATAACTACCAATGTCCTAAAATCTACATTACTGAAAATGTAATCACAGAGGCAAGAAAAGACACACTTGCACTCGATGCACAACTCAGGGATCCGCATAGAATCGAATGCATTCTTGGCAACGAAGAATGGGGTGAATGTCAAAGGCTATATCCACTGGGCTGCATTCAACGACTGGGAGTGGTCGGAAGGCTATCTTGCAAGGTTCGGGCTTTACTATGTCGACTACAAAGACAATCTCAAGCGCATTTCTTAAGAATCTGCTAA

Protein Analysis

281

Amino Acids

30.67

Weight (kDa)

8.91

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 177 - 228 3.1e-06 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14870
fragaria_vesca FvH4_5g07900
malus_domestica MD13G1193800.v1.1 MD16G1194500.v1.1
prunus_persica Prupe.1G014400_v2.0.a1
pyrus_communis pycom13g16610
rosa_chinensis RchiOBHm_Chr7g0190521
rosa_laevigata RLG00000004521
rosa_multiflora Rmu_co8215220.1_g000001 Rmu_sc0000526.1_g000006
rosa_roxburghii Rroxscaffold_3G00264540
rosa_rugosa Rorug06G0505000
rosa_samantha Rh7AG111100 Rh7BG114000 Rh7DG114900
rosa_wichuraiana Rw7G009700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 142
AccI GTMKAC 3 cut(s) 165, 525, 799
AciI CCGC 8 cut(s) 137, 140, 303, 306, 321, 329, 506, 671
AclWI GGATC 4 cut(s) 228, 241, 662, 675
AcoI YGGCCR 1 cut(s) 447
AcuI CTGAAG 2 cut(s) 134, 536
AfiI CCNNNNNNNGG 2 cut(s) 28, 362
AflII CTTAAG 1 cut(s) 829
AgsI TTSAA 4 cut(s) 57, 153, 565, 746
AjiI CACGTC 1 cut(s) 101
AjnI CCWGG 1 cut(s) 21
AjuI GAANNNNNNNTTGG 2 cut(s) 675, 707
AluBI AGCT 2 cut(s) 454, 559
AluI AGCT 2 cut(s) 454, 559
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 4 cut(s) 228, 241, 662, 675
AoxI GGCC 2 cut(s) 353, 447
ApeKI GCWGC 1 cut(s) 738
ArsI GACNNNNNNTTYG 2 cut(s) 452, 484
Asp700I GAANNNNTTC 1 cut(s) 687
AspLEI GCGC 2 cut(s) 7, 823
AspS9I GGNCC 2 cut(s) 25, 503
AsuC2I CCSGG 2 cut(s) 29, 464
AsuHPI GGTGA 4 cut(s) 30, 43, 101, 722
AvaII GGWCC 2 cut(s) 25, 503
BamHI GGATCC 2 cut(s) 233, 667
BarI GAAGNNNNNNTAC 2 cut(s) 566, 598
BbsI GAAGAC 1 cut(s) 542
BbvI GCAGC 1 cut(s) 725
BccI CCATC 2 cut(s) 262, 493
BceAI ACGGC 4 cut(s) 146, 199, 240, 425
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 2 cut(s) 29, 464
BcoDI GTCTC 1 cut(s) 107
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 829
BglI GCCNNNNNGGC 1 cut(s) 446
BisI GCNGC 5 cut(s) 137, 140, 304, 307, 739
BlsI GCNGC 5 cut(s) 138, 141, 305, 308, 740
Bme1390I CCNGG 3 cut(s) 23, 29, 464
Bme18I GGWCC 2 cut(s) 25, 503
BmgBI CACGTC 1 cut(s) 101
BmgT120I GGNCC 2 cut(s) 25, 503
BmiI GGNNCC 5 cut(s) 27, 235, 412, 504, 669
BmrFI CCNGG 3 cut(s) 23, 29, 464
BmrI ACTGGG 2 cut(s) 743, 761
BmsI GCATC 2 cut(s) 472, 643
BmuI ACTGGG 2 cut(s) 743, 761
BpiI GAAGAC 1 cut(s) 542
BpmI CTGGAG 1 cut(s) 397
BpuEI CTTGAG 1 cut(s) 801
BpuMI CCSGG 2 cut(s) 29, 464
BsaBI GATNNNNATC 1 cut(s) 122
BsaJI CCNNGG 2 cut(s) 21, 195
BsaWI WCCGGW 1 cut(s) 383
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 335, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 362
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 3 cut(s) 414, 738, 756
Bse8I GATNNNNATC 1 cut(s) 122
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 21, 195
BseJI GATNNNNATC 1 cut(s) 122
BseLI CCNNNNNNNGG 2 cut(s) 28, 362
BseMII CTCAG 1 cut(s) 676
BseNI ACTGG 3 cut(s) 414, 738, 756
BseRI GAGGAG 4 cut(s) 67, 134, 287, 347
BseXI GCAGC 1 cut(s) 725
Bsh1285I CGRYCG 1 cut(s) 463
BshFI GGCC 2 cut(s) 355, 449
BsiEI CGRYCG 1 cut(s) 463
BsiSI CCGG 6 cut(s) 29, 63, 237, 384, 463, 474
BslFI GGGAC 2 cut(s) 11, 391
BslI CCNNNNNNNGG 2 cut(s) 28, 362
BsmAI GTCTC 1 cut(s) 107
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 2 cut(s) 11, 391
BsmI GAATGC 3 cut(s) 687, 689, 741
BsnI GGCC 2 cut(s) 355, 449
Bsp143I GATC 4 cut(s) 117, 123, 233, 667
BspACI CCGC 8 cut(s) 137, 140, 303, 306, 321, 329, 506, 671
BspANI GGCC 2 cut(s) 355, 449
BspCNI CTCAG 1 cut(s) 675
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 5 cut(s) 27, 235, 412, 504, 669
BspPI GGATC 4 cut(s) 228, 241, 662, 675
BspQI GCTCTTC 1 cut(s) 204
BspTI CTTAAG 1 cut(s) 829
BsrBI CCGCTC 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 3 cut(s) 414, 738, 756
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 2 cut(s) 21, 195
BssMI GATC 4 cut(s) 117, 123, 233, 667
BssNAI GTATAC 1 cut(s) 526
Bst1107I GTATAC 1 cut(s) 526
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 433
Bst6I CTCTTC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 829
BstAPI GCANNNNNTGC 1 cut(s) 653
BstC8I GCNNGC 3 cut(s) 409, 475, 510
BstDEI CTNAG 1 cut(s) 662
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 823
BstKTI GATC 4 cut(s) 120, 126, 236, 670
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 4 cut(s) 117, 123, 233, 667
BstMCI CGRYCG 1 cut(s) 463
BstMWI GCNNNNNNNGC 3 cut(s) 446, 653, 693
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 3 cut(s) 21, 27, 462
BstV1I GCAGC 1 cut(s) 725
BstV2I GAAGAC 1 cut(s) 542
BstX2I RGATCY 2 cut(s) 233, 667
BstYI RGATCY 2 cut(s) 233, 667
BstZ17I GTATAC 1 cut(s) 526
BsuRI GGCC 2 cut(s) 355, 449
BtgZI GCGATG 1 cut(s) 281
BtrI CACGTC 1 cut(s) 101
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 4 cut(s) 86, 421, 429, 731
Cac8I GCNNGC 3 cut(s) 409, 475, 510
CciI TCATGA 1 cut(s) 570
CfoI GCGC 2 cut(s) 7, 823
Cfr10I RCCGGY 1 cut(s) 473
Cfr13I GGNCC 2 cut(s) 25, 503
CviAII CATG 1 cut(s) 571
DdeI CTNAG 1 cut(s) 662
DpnI GATC 4 cut(s) 119, 125, 235, 669
DpnII GATC 4 cut(s) 117, 123, 233, 667
EaeI YGGCCR 1 cut(s) 447
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
EciI GGCGGA 1 cut(s) 344
Eco47I GGWCC 2 cut(s) 25, 503
Eco57I CTGAAG 2 cut(s) 134, 536
EcoO109I RGGNCCY 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 689
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 1 cut(s) 574
FaiI YATR 7 cut(s) 526, 538, 572, 579, 675, 727, 796
FalI AAGNNNNNCTT 4 cut(s) 627, 659, 757, 789
FaqI GGGAC 2 cut(s) 11, 391
FatI CATG 1 cut(s) 570
FblI GTMKAC 3 cut(s) 165, 525, 799
Fnu4HI GCNGC 5 cut(s) 137, 140, 304, 307, 739
Fsp4HI GCNGC 5 cut(s) 137, 140, 304, 307, 739
GlaI GCGC 2 cut(s) 6, 822
GluI GCNGC 5 cut(s) 137, 140, 304, 307, 739
GsuI CTGGAG 1 cut(s) 397
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 355, 449
HapII CCGG 6 cut(s) 29, 63, 237, 384, 463, 474
HhaI GCGC 2 cut(s) 7, 823
Hin1II CATG 1 cut(s) 574
Hin6I GCGC 2 cut(s) 5, 821
HinP1I GCGC 2 cut(s) 5, 821
HincII GTYRAC 2 cut(s) 166, 800
HindII GTYRAC 2 cut(s) 166, 800
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 3 cut(s) 492, 678, 834
HpaII CCGG 6 cut(s) 29, 63, 237, 384, 463, 474
HphI GGTGA 4 cut(s) 30, 43, 101, 722
Hpy166II GTNNAC 3 cut(s) 166, 526, 800
Hpy188I TCNGA 5 cut(s) 187, 253, 460, 555, 764
Hpy188III TCNNGA 4 cut(s) 121, 127, 496, 571
Hpy8I GTNNAC 3 cut(s) 166, 526, 800
Hpy99I CGWCG 4 cut(s) 167, 170, 220, 226
HpyAV CCTTC 6 cut(s) 52, 256, 274, 280, 290, 759
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 100
HpyCH4V TGCA 7 cut(s) 91, 485, 647, 656, 687, 741, 777
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 653, 693
HpyF3I CTNAG 1 cut(s) 662
HpySE526I ACGT 1 cut(s) 100
Hsp92II CATG 1 cut(s) 574
HspAI GCGC 2 cut(s) 5, 821
KroI GCCGGC 1 cut(s) 473
KroNI GCCGGC 1 cut(s) 475
Kzo9I GATC 4 cut(s) 117, 123, 233, 667
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 2 cut(s) 147, 416
Lsp1109I GCAGC 1 cut(s) 725
LweI GCATC 2 cut(s) 472, 643
MaeII ACGT 1 cut(s) 100
MaeIII GTNAC 2 cut(s) 36, 82
MalI GATC 4 cut(s) 119, 125, 235, 669
MbiI CCGCTC 1 cut(s) 142
MboI GATC 4 cut(s) 117, 123, 233, 667
MboII GAAGA 6 cut(s) 45, 127, 218, 221, 542, 713
MflI RGATCY 2 cut(s) 233, 667
MlyI GAGTC 1 cut(s) 501
MmeI TCCRAC 3 cut(s) 231, 483, 742
Mph1103I ATGCAT 1 cut(s) 689
MroNI GCCGGC 1 cut(s) 473
MroXI GAANNNNTTC 1 cut(s) 687
MseI TTAA 2 cut(s) 435, 830
MspCI CTTAAG 1 cut(s) 829
MspI CCGG 6 cut(s) 29, 63, 237, 384, 463, 474
MspR9I CCNGG 3 cut(s) 23, 29, 464
Mva1269I GAATGC 3 cut(s) 687, 689, 741
MvaI CCWGG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 446, 653, 693
NaeI GCCGGC 1 cut(s) 475
NciI CCSGG 2 cut(s) 29, 464
NdeII GATC 4 cut(s) 117, 123, 233, 667
NgoMIV GCCGGC 1 cut(s) 473
NlaIII CATG 1 cut(s) 574
NlaIV GGNNCC 5 cut(s) 27, 235, 412, 504, 669
NmeAIII GCCGAG 1 cut(s) 475
NmuCI GTSAC 2 cut(s) 36, 82
NsiI ATGCAT 1 cut(s) 689
PagI TCATGA 1 cut(s) 570
PciSI GCTCTTC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PctI GAATGC 3 cut(s) 687, 689, 741
PdiI GCCGGC 1 cut(s) 475
PdmI GAANNNNTTC 1 cut(s) 687
PfeI GAWTC 2 cut(s) 678, 834
PkrI GCNGC 5 cut(s) 138, 141, 305, 308, 740
PleI GAGTC 1 cut(s) 500
PpsI GAGTC 1 cut(s) 500
PpuMI RGGWCCY 1 cut(s) 25
Psp5II RGGWCCY 1 cut(s) 25
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 5 cut(s) 27, 235, 412, 504, 669
PspPI GGNCC 2 cut(s) 25, 503
PspPPI RGGWCCY 1 cut(s) 25
PsuI RGATCY 2 cut(s) 233, 667
SalI GTCGAC 2 cut(s) 164, 798
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 2 cut(s) 435, 830
SatI GCNGC 5 cut(s) 137, 140, 304, 307, 739
Sau3AI GATC 4 cut(s) 117, 123, 233, 667
Sau96I GGNCC 2 cut(s) 25, 503
SchI GAGTC 1 cut(s) 501
ScrFI CCNGG 3 cut(s) 23, 29, 464
SfaNI GCATC 2 cut(s) 472, 643
SgrDI CGTCGACG 1 cut(s) 164
SinI GGWCC 2 cut(s) 25, 503
SmlI CTYRAG 2 cut(s) 816, 829
SmoI CTYRAG 2 cut(s) 816, 829
SsiI CCGC 8 cut(s) 137, 140, 303, 306, 321, 329, 506, 671
StyD4I CCNGG 3 cut(s) 21, 27, 462
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 1 cut(s) 103
TaqI TCGA 4 cut(s) 165, 651, 681, 799
TauI GCSGC 4 cut(s) 139, 142, 306, 309
TfiI GAWTC 2 cut(s) 678, 834
Tru1I TTAA 2 cut(s) 435, 830
Tru9I TTAA 2 cut(s) 435, 830
TscAI CASTG 4 cut(s) 93, 421, 436, 738
TseFI GTSAC 2 cut(s) 36, 82
TseI GCWGC 1 cut(s) 738
Tsp45I GTSAC 2 cut(s) 36, 82
TspDTI ATGAA 3 cut(s) 525, 559, 587
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 4 cut(s) 93, 421, 436, 738
Vha464I CTTAAG 1 cut(s) 829
VpaK11BI GGWCC 2 cut(s) 25, 503
XmiI GTMKAC 3 cut(s) 165, 525, 799
XmnI GAANNNNTTC 1 cut(s) 687
Zsp2I ATGCAT 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.