MD16G1194500.v1.1

Preprotein translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015468
Physical Location & Seq
Reverse (-)
17241633 .. 17242178
546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1194500.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGCTCTACGCATGTTCGTACAGTTCCCGGTTACCTCACCTTTCCCGCCACCCACAAAATCCGGTCACCTGAACTCATCCGTACGGTTGAAACCTCCATGTGCCGGTAGCCACTCAATTCCCCTATCACAACCCAGACGGCCCGTAACACAGAAGAACAGAAGGCAATCATTTCTCAAAGCCGTCGACGAAAGCCAGGCGAATTCCGAGTCCGGCGCCGAATCGGTGAGCACCCAAGACAAAGAATCGGCCGCGGAAGGAGAATCGGCGGCGAGTCAACCCGTCGAGTTGAGCGAGTTGGGGAAGGAGATCAAGGCGGCGATGCAGAAGAGGAAGGAGGAGAAAGAGGGAGGCTTTTTGAGCGGAGTGGTGGAGGAGGTAAGGGAGATCGAGTGGCCGGCGTTCGCTAAGGTGTTGGGGACCACCGGGGTGGTTCTTGCGGTCATTGCCGGTTCCAGCGTCGTGTTGCTCACTGTCAATGCCATTTTGGCTGAGTTTTCCGATCAGGTTTTCGCCGGGAGAGGGGTTCAGGATTTTTTCAGCTGA

Protein Analysis

182

Amino Acids

19.39

Weight (kDa)

6.17

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14870
fragaria_vesca FvH4_5g07900
malus_domestica MD13G1193800.v1.1 MD16G1194500.v1.1
prunus_persica Prupe.1G014400_v2.0.a1
pyrus_communis pycom13g16610
rosa_chinensis RchiOBHm_Chr7g0190521
rosa_laevigata RLG00000004521
rosa_multiflora Rmu_co8215220.1_g000001 Rmu_sc0000526.1_g000006
rosa_roxburghii Rroxscaffold_3G00264540
rosa_rugosa Rorug06G0505000
rosa_samantha Rh7AG111100 Rh7BG114000 Rh7DG114900
rosa_wichuraiana Rw7G009700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 215
AccBSI CCGCTC 1 cut(s) 363
AccI GTMKAC 1 cut(s) 186
AccII CGCG 1 cut(s) 254
AciI CCGC 7 cut(s) 47, 252, 254, 269, 317, 363, 440
AcoI YGGCCR 2 cut(s) 249, 395
AcsI RAATTY 1 cut(s) 202
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 2 cut(s) 21, 84
AfiI CCNNNNNNNGG 2 cut(s) 104, 522
AgsI TTSAA 1 cut(s) 91
AjnI CCWGG 1 cut(s) 195
AleI CACNNNNGTG 1 cut(s) 428
AluBI AGCT 1 cut(s) 543
AluI AGCT 1 cut(s) 543
Alw21I GWGCWC 1 cut(s) 233
AoxI GGCC 3 cut(s) 140, 249, 395
ApoI RAATTY 1 cut(s) 202
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 2 cut(s) 141, 420
AsuC2I CCSGG 3 cut(s) 29, 427, 517
AsuHPI GGTGA 3 cut(s) 30, 59, 238
AvaII GGWCC 1 cut(s) 420
BanI GGYRCC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 233
BceAI ACGGC 2 cut(s) 155, 167
BciT130I CCWGG 1 cut(s) 197
BcnI CCSGG 3 cut(s) 29, 427, 517
BfoI RGCGCY 1 cut(s) 219
BglI GCCNNNNNGGC 1 cut(s) 488
BisI GCNGC 3 cut(s) 252, 270, 318
BlsI GCNGC 3 cut(s) 253, 271, 319
Bme1390I CCNGG 4 cut(s) 29, 197, 427, 517
Bme18I GGWCC 1 cut(s) 420
BmgT120I GGNCC 2 cut(s) 141, 420
BmiI GGNNCC 3 cut(s) 217, 421, 454
BmrFI CCNGG 4 cut(s) 29, 197, 427, 517
BmsI GCATC 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 408
BpuMI CCSGG 3 cut(s) 29, 427, 517
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 2 cut(s) 252, 426
BsaWI WCCGGW 1 cut(s) 62
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 522
Bse118I RCCGGY 3 cut(s) 104, 397, 449
Bse3DI GCAATG 1 cut(s) 444
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 2 cut(s) 252, 426
BseGI GGATG 1 cut(s) 77
BseLI CCNNNNNNNGG 2 cut(s) 104, 522
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 1 cut(s) 483
BseRI GAGGAG 2 cut(s) 353, 389
BseX3I CGGCCG 1 cut(s) 249
Bsh1236I CGCG 1 cut(s) 254
Bsh1285I CGRYCG 1 cut(s) 252
BshFI GGCC 3 cut(s) 142, 251, 397
BshNI GGYRCC 1 cut(s) 215
BsiEI CGRYCG 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 233
BsiSI CCGG 8 cut(s) 29, 63, 105, 213, 398, 426, 450, 516
BsiWI CGTACG 1 cut(s) 82
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 2 cut(s) 104, 522
BsmFI GGGAC 1 cut(s) 433
BsnI GGCC 3 cut(s) 142, 251, 397
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 3 cut(s) 309, 387, 502
BspACI CCGC 7 cut(s) 47, 252, 254, 269, 317, 363, 440
BspANI GGCC 3 cut(s) 142, 251, 397
BspCNI CTCAG 1 cut(s) 484
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 3 cut(s) 217, 421, 454
BspT107I GGYRCC 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 444
BsrFI RCCGGY 3 cut(s) 104, 397, 449
BssAI RCCGGY 3 cut(s) 104, 397, 449
BssECI CCNNGG 2 cut(s) 252, 426
BssMI GATC 3 cut(s) 309, 387, 502
BssNI GRCGYC 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 3 cut(s) 24, 87, 475
Bst6I CTCTTC 1 cut(s) 323
BstACI GRCGYC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 408, 492
BstDSI CCRYGG 1 cut(s) 252
BstEII GGTNACC 2 cut(s) 31, 65
BstF5I GGATG 1 cut(s) 77
BstFNI CGCG 1 cut(s) 254
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 3 cut(s) 312, 390, 505
BstMBI GATC 3 cut(s) 309, 387, 502
BstMCI CGRYCG 1 cut(s) 252
BstMWI GCNNNNNNNGC 3 cut(s) 360, 446, 488
BstNI CCWGG 1 cut(s) 197
BstNSI RCATGY 1 cut(s) 16
BstPI GGTNACC 2 cut(s) 31, 65
BstSCI CCNGG 4 cut(s) 27, 195, 425, 515
BstUI CGCG 1 cut(s) 254
BstXI CCANNNNNNTGG 1 cut(s) 430
BstZI CGGCCG 1 cut(s) 249
BsuRI GGCC 3 cut(s) 142, 251, 397
BtgI CCRYGG 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 335
BtsCI GGATG 1 cut(s) 77
BtsIMutI CAGTG 1 cut(s) 471
Cac8I GCNNGC 1 cut(s) 399
CfoI GCGC 1 cut(s) 218
Cfr10I RCCGGY 3 cut(s) 104, 397, 449
Cfr13I GGNCC 2 cut(s) 141, 420
Cfr42I CCGCGG 1 cut(s) 255
CseI GACGC 1 cut(s) 448
Csp6I GTAC 2 cut(s) 20, 83
CviAII CATG 2 cut(s) 13, 99
CviQI GTAC 2 cut(s) 20, 83
DdeI CTNAG 2 cut(s) 408, 492
DinI GGCGCC 1 cut(s) 217
DpnI GATC 3 cut(s) 311, 389, 504
DpnII GATC 3 cut(s) 309, 387, 502
EaeI YGGCCR 2 cut(s) 249, 395
EagI CGGCCG 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
EclXI CGGCCG 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 420
Eco52I CGGCCG 1 cut(s) 249
Eco91I GGTNACC 2 cut(s) 31, 65
EcoO65I GGTNACC 2 cut(s) 31, 65
EcoRI GAATTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 195
EgeI GGCGCC 1 cut(s) 217
EheI GGCGCC 1 cut(s) 217
FaeI CATG 2 cut(s) 16, 102
FaiI YATR 2 cut(s) 14, 100
FaqI GGGAC 1 cut(s) 433
FatI CATG 2 cut(s) 12, 98
FauI CCCGC 1 cut(s) 54
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 3 cut(s) 252, 270, 318
FokI GGATG 1 cut(s) 64
Fsp4HI GCNGC 3 cut(s) 252, 270, 318
GlaI GCGC 1 cut(s) 217
GluI GCNGC 3 cut(s) 252, 270, 318
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 3 cut(s) 142, 251, 397
HapII CCGG 8 cut(s) 29, 63, 105, 213, 398, 426, 450, 516
HgaI GACGC 1 cut(s) 448
HhaI GCGC 1 cut(s) 218
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 2 cut(s) 16, 102
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HincII GTYRAC 2 cut(s) 187, 278
HindII GTYRAC 2 cut(s) 187, 278
HinfI GANTC 5 cut(s) 209, 221, 245, 263, 274
HpaII CCGG 8 cut(s) 29, 63, 105, 213, 398, 426, 450, 516
HphI GGTGA 3 cut(s) 30, 59, 238
Hpy166II GTNNAC 2 cut(s) 187, 278
Hpy188I TCNGA 2 cut(s) 208, 502
Hpy188III TCNNGA 1 cut(s) 530
Hpy8I GTNNAC 2 cut(s) 187, 278
Hpy99I CGWCG 4 cut(s) 188, 191, 287, 464
HpyAV CCTTC 4 cut(s) 156, 251, 298, 328
HpyCH4III ACNGT 3 cut(s) 24, 87, 475
HpyCH4V TGCA 1 cut(s) 325
HpyF10VI GCNNNNNNNGC 3 cut(s) 360, 446, 488
HpyF3I CTNAG 2 cut(s) 408, 492
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 2 cut(s) 16, 102
HspAI GCGC 1 cut(s) 216
KasI GGCGCC 1 cut(s) 215
KroI GCCGGC 1 cut(s) 397
KroNI GCCGGC 1 cut(s) 399
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 3 cut(s) 309, 387, 502
LweI GCATC 1 cut(s) 312
MaeIII GTNAC 3 cut(s) 31, 65, 145
MalI GATC 3 cut(s) 311, 389, 504
MbiI CCGCTC 1 cut(s) 363
MboI GATC 3 cut(s) 309, 387, 502
MboII GAAGA 2 cut(s) 166, 340
MhlI GDGCHC 1 cut(s) 233
MluCI AATT 2 cut(s) 117, 202
Mly113I GGCGCC 1 cut(s) 216
MlyI GAGTC 2 cut(s) 218, 283
MnlI CCTC 9 cut(s) 46, 105, 324, 331, 340, 344, 367, 370, 515
MroNI GCCGGC 1 cut(s) 397
MslI CAYNNNNRTG 1 cut(s) 428
MspA1I CMGCKG 2 cut(s) 254, 543
MspI CCGG 8 cut(s) 29, 63, 105, 213, 398, 426, 450, 516
MspR9I CCNGG 4 cut(s) 29, 197, 427, 517
MvaI CCWGG 1 cut(s) 197
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 3 cut(s) 360, 446, 488
NaeI GCCGGC 1 cut(s) 399
NarI GGCGCC 1 cut(s) 216
NciI CCSGG 3 cut(s) 29, 427, 517
NdeII GATC 3 cut(s) 309, 387, 502
NgoMIV GCCGGC 1 cut(s) 397
NlaIII CATG 2 cut(s) 16, 102
NlaIV GGNNCC 3 cut(s) 217, 421, 454
NmuCI GTSAC 1 cut(s) 65
NspI RCATGY 1 cut(s) 16
OliI CACNNNNGTG 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PdiI GCCGGC 1 cut(s) 399
PfeI GAWTC 3 cut(s) 221, 245, 263
Pfl23II CGTACG 1 cut(s) 82
PkrI GCNGC 3 cut(s) 253, 271, 319
PleI GAGTC 2 cut(s) 217, 282
PluTI GGCGCC 1 cut(s) 219
PpsI GAGTC 2 cut(s) 217, 282
Psp6I CCWGG 1 cut(s) 195
PspEI GGTNACC 2 cut(s) 31, 65
PspGI CCWGG 1 cut(s) 195
PspLI CGTACG 1 cut(s) 82
PspN4I GGNNCC 3 cut(s) 217, 421, 454
PspPI GGNCC 2 cut(s) 141, 420
PvuII CAGCTG 1 cut(s) 543
RsaI GTAC 2 cut(s) 21, 84
RsaNI GTAC 2 cut(s) 20, 83
RseI CAYNNNNRTG 1 cut(s) 428
SacII CCGCGG 1 cut(s) 255
SalI GTCGAC 1 cut(s) 185
SatI GCNGC 3 cut(s) 252, 270, 318
Sau3AI GATC 3 cut(s) 309, 387, 502
Sau96I GGNCC 2 cut(s) 141, 420
SchI GAGTC 2 cut(s) 218, 283
ScrFI CCNGG 4 cut(s) 29, 197, 427, 517
SduI GDGCHC 1 cut(s) 233
SetI ASST 8 cut(s) 38, 43, 72, 97, 381, 414, 510, 545
SfaNI GCATC 1 cut(s) 312
SfoI GGCGCC 1 cut(s) 217
Sfr303I CCGCGG 1 cut(s) 255
SgrBI CCGCGG 1 cut(s) 255
SgrDI CGTCGACG 1 cut(s) 185
SinI GGWCC 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 428
Sse9I AATT 2 cut(s) 117, 202
SsiI CCGC 7 cut(s) 47, 252, 254, 269, 317, 363, 440
SspDI GGCGCC 1 cut(s) 215
StyD4I CCNGG 4 cut(s) 27, 195, 425, 515
TaaI ACNGT 3 cut(s) 24, 87, 475
TaqI TCGA 3 cut(s) 186, 285, 390
TasI AATT 2 cut(s) 117, 202
TauI GCSGC 3 cut(s) 254, 272, 320
TfiI GAWTC 3 cut(s) 221, 245, 263
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 1 cut(s) 65
Tsp45I GTSAC 1 cut(s) 65
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 420
XapI RAATTY 1 cut(s) 202
XceI RCATGY 1 cut(s) 16
XmiI GTMKAC 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.