RLG00000004521

Preprotein translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015468
Physical Location & Seq
Reverse (-)
59988166 .. 59988678
513 bp
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UTR
Exon/CDS
Intron
RLM00000004521

Sequence Viewer

Length: 513 bp
ATGGCGCTTCGGTTTCTCGCCCAAGTCCCGGTAATCTCACCCTTCCCGTCCACTTCAAAATCCGGCAACCTTACCTCCTCCTCTTCCGTCAGACTGCAACCGCCACGTGTCAGTCACCTGATGGAGAAGAGCAAGAGAAAGCCACCACTCTTCAGTTCAATCAAAGCCGTCGACGAAAGCCAAGCGGATTCCGACACCGAGCCAAAAGGAGAGTCGTCGGCGTCGGCGAGTGAACCCGGAGAGTTGAGCGAGTTGGGGGCGGAGATCAAGAAGGCCATGTTAGAGAGGAAGGAGAAGGAGAAAGAAGGAGGAGGCGGCAATTTGTTGAGTGGAGTGGCGGAGGAGATTCGGGAGATCGAGTGGCCTGTGTTCGGCAAGGTCTTGGGGACTACTGGAGTTGTGCTTGGGGTTATTGCCGGCTCCAGCGTCGTTTTGCTCACTGTCAATGCCGTTTTGGCCGAGCTGTCCGACCGGGTCTTCGCCGGCAGAGGCGTTCAGGATTTTTTCGGCTGA

Protein Analysis

171

Amino Acids

17.94

Weight (kDa)

5.58

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14870
fragaria_vesca FvH4_5g07900
malus_domestica MD13G1193800.v1.1 MD16G1194500.v1.1
prunus_persica Prupe.1G014400_v2.0.a1
pyrus_communis pycom13g16610
rosa_chinensis RchiOBHm_Chr7g0190521
rosa_laevigata RLG00000004521
rosa_multiflora Rmu_co8215220.1_g000001 Rmu_sc0000526.1_g000006
rosa_roxburghii Rroxscaffold_3G00264540
rosa_rugosa Rorug06G0505000
rosa_samantha Rh7AG111100 Rh7BG114000 Rh7DG114900
rosa_wichuraiana Rw7G009700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 171
AciI CCGC 5 cut(s) 101, 185, 260, 315, 338
AcoI YGGCCR 1 cut(s) 456
AcuI CTGAAG 1 cut(s) 136
AcvI CACGTG 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 221
AfiI CCNNNNNNNGG 2 cut(s) 28, 371
AflIII ACRYGT 1 cut(s) 106
AgsI TTSAA 2 cut(s) 57, 159
AluBI AGCT 1 cut(s) 463
AluI AGCT 1 cut(s) 463
AoxI GGCC 3 cut(s) 273, 362, 456
ArsI GACNNNNNNTTYG 4 cut(s) 197, 229, 461, 493
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 3 cut(s) 29, 237, 473
AsuHPI GGTGA 2 cut(s) 30, 107
BbrPI CACGTG 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 469
BccI CCATC 1 cut(s) 115
BceAI ACGGC 2 cut(s) 152, 434
BcnI CCSGG 3 cut(s) 29, 237, 473
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 1 cut(s) 455
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
Bme1390I CCNGG 3 cut(s) 29, 237, 473
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 3 cut(s) 29, 237, 473
BpiI GAAGAC 1 cut(s) 469
BpmI CTGGAG 2 cut(s) 406, 414
BpuMI CCSGG 3 cut(s) 29, 237, 473
BsaAI YACGTR 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 221
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 344, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 371
Bse118I RCCGGY 2 cut(s) 416, 482
Bse1I ACTGG 1 cut(s) 397
BseLI CCNNNNNNNGG 2 cut(s) 28, 371
BseNI ACTGG 1 cut(s) 397
BseRI GAGGAG 4 cut(s) 67, 70, 324, 356
Bsh1285I CGRYCG 1 cut(s) 472
BshFI GGCC 3 cut(s) 275, 364, 458
BsiEI CGRYCG 1 cut(s) 472
BsiSI CCGG 6 cut(s) 29, 63, 237, 417, 472, 483
BslFI GGGAC 2 cut(s) 11, 400
BslI CCNNNNNNNGG 2 cut(s) 28, 371
BsmFI GGGAC 2 cut(s) 11, 400
BsnI GGCC 3 cut(s) 275, 364, 458
Bsp143I GATC 2 cut(s) 264, 354
BspACI CCGC 5 cut(s) 101, 185, 260, 315, 338
BspANI GGCC 3 cut(s) 275, 364, 458
BspLI GGNNCC 1 cut(s) 421
BspQI GCTCTTC 1 cut(s) 122
BsrFI RCCGGY 2 cut(s) 416, 482
BsrI ACTGG 1 cut(s) 397
BssAI RCCGGY 2 cut(s) 416, 482
BssMI GATC 2 cut(s) 264, 354
BssNI GRCGYC 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 3 cut(s) 88, 122, 155
BstACI GRCGYC 1 cut(s) 221
BstBAI YACGTR 1 cut(s) 107
BstC8I GCNNGC 2 cut(s) 418, 484
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 267, 357
BstMBI GATC 2 cut(s) 264, 354
BstMCI CGRYCG 1 cut(s) 472
BstMWI GCNNNNNNNGC 1 cut(s) 455
BstSCI CCNGG 3 cut(s) 27, 235, 471
BstV2I GAAGAC 1 cut(s) 469
BsuRI GGCC 3 cut(s) 275, 364, 458
BtsIMutI CAGTG 1 cut(s) 438
Cac8I GCNNGC 2 cut(s) 418, 484
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 2 cut(s) 416, 482
CseI GACGC 2 cut(s) 210, 415
CviAII CATG 1 cut(s) 277
DpnI GATC 2 cut(s) 266, 356
DpnII GATC 2 cut(s) 264, 354
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 3 cut(s) 88, 122, 155
EarI CTCTTC 3 cut(s) 88, 122, 155
EciI GGCGGA 2 cut(s) 275, 353
Eco57I CTGAAG 1 cut(s) 136
Eco72I CACGTG 1 cut(s) 107
FaeI CATG 1 cut(s) 280
FaiI YATR 1 cut(s) 278
FaqI GGGAC 2 cut(s) 11, 400
FatI CATG 1 cut(s) 276
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 316
Fsp4HI GCNGC 1 cut(s) 316
GlaI GCGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 316
GsuI CTGGAG 2 cut(s) 406, 414
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 275, 364, 458
HapII CCGG 6 cut(s) 29, 63, 237, 417, 472, 483
HgaI GACGC 2 cut(s) 210, 415
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 1 cut(s) 280
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 3 cut(s) 188, 212, 346
HpaII CCGG 6 cut(s) 29, 63, 237, 417, 472, 483
HphI GGTGA 2 cut(s) 30, 107
Hpy166II GTNNAC 3 cut(s) 51, 172, 233
Hpy188I TCNGA 3 cut(s) 92, 193, 469
Hpy188III TCNNGA 3 cut(s) 268, 350, 497
Hpy8I GTNNAC 3 cut(s) 51, 172, 233
Hpy99I CGWCG 5 cut(s) 173, 176, 220, 226, 431
HpyAV CCTTC 5 cut(s) 52, 265, 283, 289, 299
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 1 cut(s) 106
HpyCH4V TGCA 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 1 cut(s) 455
HpySE526I ACGT 1 cut(s) 106
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 1 cut(s) 280
HspAI GCGC 1 cut(s) 5
KroI GCCGGC 2 cut(s) 416, 482
KroNI GCCGGC 2 cut(s) 418, 484
Kzo9I GATC 2 cut(s) 264, 354
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 1 cut(s) 425
MaeII ACGT 1 cut(s) 106
MaeIII GTNAC 1 cut(s) 113
MalI GATC 2 cut(s) 266, 356
MboI GATC 2 cut(s) 264, 354
MboII GAAGA 4 cut(s) 75, 139, 142, 469
MluCI AATT 1 cut(s) 319
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 2 cut(s) 216, 492
MnlI CCTC 8 cut(s) 85, 88, 91, 279, 302, 305, 334, 482
MroNI GCCGGC 2 cut(s) 416, 482
MspI CCGG 6 cut(s) 29, 63, 237, 417, 472, 483
MspR9I CCNGG 3 cut(s) 29, 237, 473
MwoI GCNNNNNNNGC 1 cut(s) 455
NaeI GCCGGC 2 cut(s) 418, 484
NciI CCSGG 3 cut(s) 29, 237, 473
NdeII GATC 2 cut(s) 264, 354
NgoMIV GCCGGC 2 cut(s) 416, 482
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 1 cut(s) 421
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PdiI GCCGGC 2 cut(s) 418, 484
PfeI GAWTC 2 cut(s) 188, 346
PflFI GACNNNGTC 1 cut(s) 473
PkrI GCNGC 1 cut(s) 317
PleI GAGTC 1 cut(s) 220
PmaCI CACGTG 1 cut(s) 107
PmlI CACGTG 1 cut(s) 107
PpsI GAGTC 1 cut(s) 220
Ppu21I YACGTR 1 cut(s) 107
PspCI CACGTG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 421
PsyI GACNNNGTC 1 cut(s) 473
SalI GTCGAC 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 122
SatI GCNGC 1 cut(s) 316
Sau3AI GATC 2 cut(s) 264, 354
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 3 cut(s) 29, 237, 473
SetI ASST 6 cut(s) 72, 77, 109, 120, 381, 465
SgrDI CGTCGACG 1 cut(s) 170
Sse9I AATT 1 cut(s) 319
SsiI CCGC 5 cut(s) 101, 185, 260, 315, 338
StyD4I CCNGG 3 cut(s) 27, 235, 471
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 1 cut(s) 109
TaqI TCGA 2 cut(s) 171, 357
TasI AATT 1 cut(s) 319
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 2 cut(s) 188, 346
TscAI CASTG 1 cut(s) 445
TseFI GTSAC 1 cut(s) 113
Tsp45I GTSAC 1 cut(s) 113
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 1 cut(s) 445
Tth111I GACNNNGTC 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.