FvH4_5g08180

Det1 complexing ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009125
Physical Location & Seq
Reverse (-)
4760787 .. 4763511
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08180.t2

Sequence Viewer

Length: 495 bp
ATGGAGGGCTTCTCTTCTCAGCCGTCCGATTCCAATCCAACGGCTTCCGGCGCTTCCATTTCCCTCCAAAACCTCCCCTCCCGCGGCCTCTTCTCCGCCCCCGTTCTCTCTTTGAATCCGGGTAGGATACGAGTTTGTGTCTGTGAGTACGAGACATCACTTCCAGAGGATGAACATATAAAGACAAACCAACAAAACATTTTGATTAGATCACTTAAGCTCAAGAAACAGAAGGACAATTCAAAGGATGTGAAGGGAGCAGGTGCAGCTGAAGGCTCAAGAAAGAGGGTTGCTGAGAGAGTTTTGGATAACCGGGCGCCAGCTAAGAAAGCCACTATGATACCTCGACAAGAGGAATCAAGTAGTCAAGCAGCTGGTAGGGACTTCCAGGGATTAACTGTAGAGAGGCTCCGTGCCCTTCTGAAGGCTAAAGGGCTTTCTGTAAAGGGAAAGAAGGAGGAATTGATTGCACGGCTGAGAAGTTCAAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDA1 SAP

Protein Analysis

165

Amino Acids

17.77

Weight (kDa)

10.0

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDA1 PF10172 22 - 78 2.5e-06 Det1 complexing ubiquitin ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 251
Acc36I ACCTGC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 316
AccII CGCG 1 cut(s) 84
AciI CCGC 3 cut(s) 82, 84, 96
AcuI CTGAAG 2 cut(s) 291, 443
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 83, 424
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 3 cut(s) 115, 243, 486
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 4 cut(s) 220, 269, 323, 374
AluI AGCT 4 cut(s) 220, 269, 323, 374
Alw26I GTCTC 1 cut(s) 146
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 2 cut(s) 266, 371
AspLEI GCGC 2 cut(s) 53, 319
AsuC2I CCSGG 2 cut(s) 120, 314
BaeGI GKGCMC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 316
BbvI GCAGC 2 cut(s) 278, 383
BceAI ACGGC 3 cut(s) 7, 57, 488
BciT130I CCWGG 1 cut(s) 389
BciVI GTATCC 1 cut(s) 120
BcnI CCSGG 2 cut(s) 120, 314
BcoDI GTCTC 1 cut(s) 146
BfmI CTRYAG 1 cut(s) 399
BfoI RGCGCY 2 cut(s) 54, 320
BfrI CTTAAG 1 cut(s) 215
BfuAI ACCTGC 1 cut(s) 251
BfuI GTATCC 1 cut(s) 120
BisI GCNGC 3 cut(s) 85, 267, 372
BlsI GCNGC 3 cut(s) 86, 268, 373
Bme1390I CCNGG 3 cut(s) 120, 314, 389
BmiI GGNNCC 2 cut(s) 318, 410
BmrFI CCNGG 3 cut(s) 120, 314, 389
BplI GAGNNNNNCTC 1 cut(s) 28
BpuEI CTTGAG 2 cut(s) 206, 262
BpuMI CCSGG 2 cut(s) 120, 314
BsaBI GATNNNNATC 1 cut(s) 33
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 82, 388
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 424
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 2 cut(s) 82, 388
BseGI GGATG 2 cut(s) 175, 253
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 2 cut(s) 83, 424
BseMII CTCAG 3 cut(s) 32, 285, 467
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 2 cut(s) 278, 383
BsgI GTGCAG 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 87
BshNI GGYRCC 1 cut(s) 316
BsiSI CCGG 3 cut(s) 48, 119, 313
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 2 cut(s) 83, 424
BsmAI GTCTC 1 cut(s) 146
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 1 cut(s) 209
BspACI CCGC 3 cut(s) 82, 84, 96
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 3 cut(s) 31, 286, 468
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 318, 410
BspMI ACCTGC 1 cut(s) 251
BspT107I GGYRCC 1 cut(s) 316
BspTI CTTAAG 1 cut(s) 215
BssECI CCNNGG 2 cut(s) 82, 388
BssMI GATC 1 cut(s) 209
BssNI GRCGYC 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 2 cut(s) 19, 95
BstACI GRCGYC 1 cut(s) 317
BstAFI CTTAAG 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 4 cut(s) 18, 294, 324, 476
BstDSI CCRYGG 1 cut(s) 82
BstENI CCTNNNNNAGG 1 cut(s) 422
BstF5I GGATG 2 cut(s) 175, 253
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 2 cut(s) 54, 320
BstHHI GCGC 2 cut(s) 53, 319
BstKTI GATC 1 cut(s) 212
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 1 cut(s) 209
BstMWI GCNNNNNNNGC 3 cut(s) 50, 266, 329
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 3 cut(s) 118, 312, 387
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 418
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 278, 383
BsuI GTATCC 1 cut(s) 120
BsuRI GGCC 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 2 cut(s) 175, 253
BveI ACCTGC 1 cut(s) 251
Cac8I GCNNGC 1 cut(s) 321
CfoI GCGC 2 cut(s) 53, 319
Cfr42I CCGCGG 1 cut(s) 85
Csp6I GTAC 1 cut(s) 148
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 4 cut(s) 18, 294, 324, 476
DinI GGCGCC 1 cut(s) 318
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 19, 95
EarI CTCTTC 2 cut(s) 19, 95
EciI GGCGGA 1 cut(s) 85
Eco57I CTGAAG 2 cut(s) 291, 443
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 387
EgeI GGCGCC 1 cut(s) 318
EheI GGCGCC 1 cut(s) 318
FaiI YATR 3 cut(s) 177, 179, 338
FaqI GGGAC 1 cut(s) 395
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 3 cut(s) 85, 267, 372
FokI GGATG 2 cut(s) 182, 260
Fsp4HI GCNGC 3 cut(s) 85, 267, 372
GlaI GCGC 2 cut(s) 52, 318
GluI GCNGC 3 cut(s) 85, 267, 372
HaeII RGCGCY 2 cut(s) 54, 320
HaeIII GGCC 1 cut(s) 87
HapII CCGG 3 cut(s) 48, 119, 313
HhaI GCGC 2 cut(s) 53, 319
Hin1I GRCGYC 1 cut(s) 317
Hin6I GCGC 2 cut(s) 51, 317
HinP1I GCGC 2 cut(s) 51, 317
HinfI GANTC 3 cut(s) 29, 115, 356
HpaII CCGG 3 cut(s) 48, 119, 313
Hpy188I TCNGA 2 cut(s) 28, 423
Hpy188III TCNNGA 3 cut(s) 164, 223, 279
HpyAV CCTTC 6 cut(s) 226, 247, 266, 418, 428, 448
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4V TGCA 2 cut(s) 266, 470
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 266, 329
HpyF3I CTNAG 4 cut(s) 18, 294, 324, 476
Hsp92I GRCGYC 1 cut(s) 317
HspAI GCGC 2 cut(s) 51, 317
KasI GGCGCC 1 cut(s) 316
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 1 cut(s) 209
LmnI GCTCC 2 cut(s) 257, 414
LpnPI CCDG 9 cut(s) 61, 132, 177, 246, 326, 333, 360, 374, 401
Lsp1109I GCAGC 2 cut(s) 278, 383
MalI GATC 1 cut(s) 211
MboI GATC 1 cut(s) 209
MboII GAAGA 2 cut(s) 6, 82
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 2 cut(s) 238, 461
Mly113I GGCGCC 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 62
MseI TTAA 2 cut(s) 216, 395
MspA1I CMGCKG 3 cut(s) 84, 269, 374
MspCI CTTAAG 1 cut(s) 215
MspI CCGG 3 cut(s) 48, 119, 313
MspR9I CCNGG 3 cut(s) 120, 314, 389
MvaI CCWGG 1 cut(s) 389
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 50, 266, 329
NarI GGCGCC 1 cut(s) 317
NciI CCSGG 2 cut(s) 120, 314
NdeII GATC 1 cut(s) 209
NlaIV GGNNCC 2 cut(s) 318, 410
PaqCI CACCTGC 1 cut(s) 251
PfeI GAWTC 3 cut(s) 29, 115, 356
PkrI GCNGC 3 cut(s) 86, 268, 373
PluTI GGCGCC 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 2 cut(s) 318, 410
PvuII CAGCTG 2 cut(s) 269, 374
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SacII CCGCGG 1 cut(s) 85
SaqAI TTAA 2 cut(s) 216, 395
SatI GCNGC 3 cut(s) 85, 267, 372
Sau3AI GATC 1 cut(s) 209
ScrFI CCNGG 3 cut(s) 120, 314, 389
SduI GDGCHC 1 cut(s) 418
SetI ASST 7 cut(s) 75, 222, 265, 271, 325, 346, 376
SfcI CTRYAG 1 cut(s) 399
SfoI GGCGCC 1 cut(s) 318
Sfr303I CCGCGG 1 cut(s) 85
SgrBI CCGCGG 1 cut(s) 85
SmlI CTYRAG 3 cut(s) 215, 221, 277
SmoI CTYRAG 3 cut(s) 215, 221, 277
Sse9I AATT 2 cut(s) 238, 461
SsiI CCGC 3 cut(s) 82, 84, 96
SspDI GGCGCC 1 cut(s) 316
StyD4I CCNGG 3 cut(s) 118, 312, 387
TaaI ACNGT 1 cut(s) 400
TaqI TCGA 1 cut(s) 346
TasI AATT 2 cut(s) 238, 461
TauI GCSGC 1 cut(s) 87
TfiI GAWTC 3 cut(s) 29, 115, 356
Tru1I TTAA 2 cut(s) 216, 395
Tru9I TTAA 2 cut(s) 216, 395
TseI GCWGC 2 cut(s) 266, 371
TspDTI ATGAA 1 cut(s) 186
TspGWI ACGGA 1 cut(s) 401
Vha464I CTTAAG 1 cut(s) 215
XagI CCTNNNNNAGG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.