Rroxscaffold_3G00264720

Det1 complexing ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009125
Physical Location & Seq
Reverse (-)
58260616 .. 58263585
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00264720.1

Sequence Viewer

Length: 498 bp
ATGGAGGGCTTCTCTTCTCAGCAGTCCGATTCTAATCAAACGGCTTCCGGCGCTTCCATTTCCCTCCAAAACCTCCCCTCCCGCGGCCTCTTCTCCGCTCCCGTTCTCTCTTTGAATCCGGGTAGGATGCGAGTTTGTGTCTGTGAGTACGAGACATCACTCCCAGAGGATGAACATATAAAAACAAACCAACAAAACATATTGATTAGATCACTCAAGCTTAAGAAACAGAAGGGATATTCCAGTTCAAAGGATGTGAAGGGTGCAGGTGCAGCTGAAGGCTCAAGAAAGAGGGTTGCTGAAAGAGTTTTGGACCGGGTGCCAGCTAAGAAAGCCTCTATGCAACCTCGTCAAGAGGAATCAAGCAGTCAGGCATCTAGTAGGGACTTCCAGGGATTAACTGTAGAGAGGCTCCGTAACCTTTTGAAGGCTAAAGGTCTTTCTGTAAAGGGAAAAAAGGAGGAATTGATTGCACGGCTGAGGAGTTCAAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDA1 SAP

Protein Analysis

165

Amino Acids

18.02

Weight (kDa)

10.05

Isoelectric Point (pI)

56.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDA1 PF10172 22 - 78 3.3e-07 Det1 complexing ubiquitin ligase
SAP PF02037 129 - 161 7e-10 SAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 257
AccB1I GGYRCC 1 cut(s) 319
AccBSI CCGCTC 1 cut(s) 98
AccII CGCG 1 cut(s) 84
AciI CCGC 3 cut(s) 82, 84, 96
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 83, 427
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 4 cut(s) 115, 249, 427, 489
AjnI CCWGG 1 cut(s) 390
AluBI AGCT 3 cut(s) 220, 275, 326
AluI AGCT 3 cut(s) 220, 275, 326
Alw26I GTCTC 1 cut(s) 146
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 313
AsuC2I CCSGG 2 cut(s) 120, 317
AvaII GGWCC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 319
BbvCI CCTCAGC 1 cut(s) 479
BbvI GCAGC 1 cut(s) 284
BceAI ACGGC 2 cut(s) 57, 491
BciT130I CCWGG 1 cut(s) 392
BcnI CCSGG 2 cut(s) 120, 317
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 402
BfoI RGCGCY 1 cut(s) 54
BfrI CTTAAG 1 cut(s) 221
BfuAI ACCTGC 1 cut(s) 257
BisI GCNGC 2 cut(s) 85, 273
BlsI GCNGC 2 cut(s) 86, 274
Bme1390I CCNGG 3 cut(s) 120, 317, 392
Bme18I GGWCC 1 cut(s) 313
BmgT120I GGNCC 1 cut(s) 313
BmiI GGNNCC 2 cut(s) 321, 413
BmrFI CCNGG 3 cut(s) 120, 317, 392
BmsI GCATC 2 cut(s) 117, 383
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu10I CCTNAGC 1 cut(s) 479
BpuEI CTTGAG 2 cut(s) 200, 268
BpuMI CCSGG 2 cut(s) 120, 317
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 2 cut(s) 82, 391
BsaXI ACNNNNNCTCC 3 cut(s) 62, 92, 475
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 427
Bse1I ACTGG 1 cut(s) 243
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 2 cut(s) 82, 391
BseGI GGATG 3 cut(s) 132, 175, 259
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 2 cut(s) 83, 427
BseMII CTCAG 2 cut(s) 32, 470
BseNI ACTGG 1 cut(s) 243
BseRI GAGGAG 1 cut(s) 496
BseXI GCAGC 1 cut(s) 284
BsgI GTGCAG 2 cut(s) 285, 291
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 87
BshNI GGYRCC 1 cut(s) 319
BsiSI CCGG 3 cut(s) 48, 119, 316
BslFI GGGAC 1 cut(s) 398
BslI CCNNNNNNNGG 2 cut(s) 83, 427
BsmAI GTCTC 1 cut(s) 146
BsmFI GGGAC 1 cut(s) 398
BsnI GGCC 1 cut(s) 87
Bsp143I GATC 1 cut(s) 209
BspACI CCGC 3 cut(s) 82, 84, 96
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 2 cut(s) 31, 471
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 321, 413
BspMI ACCTGC 1 cut(s) 257
BspT107I GGYRCC 1 cut(s) 319
BspTI CTTAAG 1 cut(s) 221
BsrBI CCGCTC 1 cut(s) 98
BsrI ACTGG 1 cut(s) 243
BssECI CCNNGG 2 cut(s) 82, 391
BssMI GATC 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 2 cut(s) 19, 95
BstAFI CTTAAG 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 3 cut(s) 18, 327, 479
BstDSI CCRYGG 1 cut(s) 82
BstENI CCTNNNNNAGG 1 cut(s) 425
BstF5I GGATG 3 cut(s) 132, 175, 259
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 1 cut(s) 212
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 1 cut(s) 209
BstMWI GCNNNNNNNGC 3 cut(s) 50, 272, 332
BstNI CCWGG 1 cut(s) 392
BstSCI CCNGG 3 cut(s) 118, 315, 390
BstSFI CTRYAG 1 cut(s) 402
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 284
BsuRI GGCC 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 3 cut(s) 132, 175, 259
BveI ACCTGC 1 cut(s) 257
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 313
Cfr42I CCGCGG 1 cut(s) 85
Csp6I GTAC 1 cut(s) 148
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 3 cut(s) 18, 327, 479
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 19, 95
EarI CTCTTC 2 cut(s) 19, 95
Eco47I GGWCC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 297
EcoNI CCTNNNNNAGG 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 390
FaiI YATR 4 cut(s) 177, 179, 200, 341
FaqI GGGAC 1 cut(s) 398
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 2 cut(s) 85, 273
FokI GGATG 3 cut(s) 139, 182, 266
Fsp4HI GCNGC 2 cut(s) 85, 273
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 52
GluI GCNGC 2 cut(s) 85, 273
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 87
HapII CCGG 3 cut(s) 48, 119, 316
HhaI GCGC 1 cut(s) 53
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 3 cut(s) 29, 115, 359
HpaII CCGG 3 cut(s) 48, 119, 316
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 2 cut(s) 285, 353
HpyAV CCTTC 4 cut(s) 226, 253, 272, 421
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4V TGCA 4 cut(s) 266, 272, 343, 473
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 272, 332
HpyF3I CTNAG 3 cut(s) 18, 327, 479
HspAI GCGC 1 cut(s) 51
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 1 cut(s) 209
LmnI GCTCC 2 cut(s) 103, 417
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 2 cut(s) 117, 383
MaeI CTAG 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 416
MalI GATC 1 cut(s) 211
MbiI CCGCTC 1 cut(s) 98
MboI GATC 1 cut(s) 209
MboII GAAGA 2 cut(s) 6, 82
MluCI AATT 1 cut(s) 464
MseI TTAA 2 cut(s) 222, 398
MspA1I CMGCKG 2 cut(s) 84, 275
MspCI CTTAAG 1 cut(s) 221
MspI CCGG 3 cut(s) 48, 119, 316
MspR9I CCNGG 3 cut(s) 120, 317, 392
MvaI CCWGG 1 cut(s) 392
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 50, 272, 332
NciI CCSGG 2 cut(s) 120, 317
NdeII GATC 1 cut(s) 209
NlaIV GGNNCC 2 cut(s) 321, 413
PaqCI CACCTGC 1 cut(s) 257
PfeI GAWTC 3 cut(s) 29, 115, 359
PkrI GCNGC 2 cut(s) 86, 274
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 2 cut(s) 321, 413
PspPI GGNCC 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SacII CCGCGG 1 cut(s) 85
SaqAI TTAA 2 cut(s) 222, 398
SatI GCNGC 2 cut(s) 85, 273
Sau3AI GATC 1 cut(s) 209
Sau96I GGNCC 1 cut(s) 313
ScrFI CCNGG 3 cut(s) 120, 317, 392
SetI ASST 8 cut(s) 75, 222, 271, 277, 328, 349, 423, 439
SfaNI GCATC 2 cut(s) 117, 383
SfcI CTRYAG 1 cut(s) 402
Sfr303I CCGCGG 1 cut(s) 85
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 1 cut(s) 313
SmlI CTYRAG 3 cut(s) 215, 221, 283
SmoI CTYRAG 3 cut(s) 215, 221, 283
Sse9I AATT 1 cut(s) 464
SsiI CCGC 3 cut(s) 82, 84, 96
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 3 cut(s) 118, 315, 390
TaaI ACNGT 1 cut(s) 403
TasI AATT 1 cut(s) 464
TauI GCSGC 1 cut(s) 87
TfiI GAWTC 3 cut(s) 29, 115, 359
Tru1I TTAA 2 cut(s) 222, 398
Tru9I TTAA 2 cut(s) 222, 398
TseI GCWGC 1 cut(s) 272
TspDTI ATGAA 1 cut(s) 186
TspGWI ACGGA 1 cut(s) 404
Vha464I CTTAAG 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 313
XagI CCTNNNNNAGG 1 cut(s) 425
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.