Rh7AG109600

Det1 complexing ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0009125
Physical Location & Seq
Forward (+)
8626018 .. 8629230
3213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG109600.1

Sequence Viewer

Length: 429 bp
ATGGAGGGCTTCTCTTCTCAGCCGTCCGATTCCAATCAAAAGGCTTCCGGCGCTTCCATTTCCCTTCAAAACCTCCCCTCCCGCGGCCTCTTCTCCGCCCCCGTTCTCTCTTTGAATCCGGGTAGGATGCGAGTTTGTGTCTGTGAGTATGAGACATCACTCCCAGAGGATGAACATATAAAAACAAACCAACAAAACATATTGATTAGATCACTTAAGCTTAAGAAACAGAAGGAGAAGGGAGATTCCAGTTCAAAGGATGTGAAGGGTGCAGGTGCAGCTGAGGGCTCAAGAAAGAGGTATGACCAGTATCCTTTGGGGATGGACCATGTTGGGAATTTCTTGATAAGAAAATGTCTTTGTGATTCAATTGAATTTTTATTTGATAAGATCAAGTTCATAAACTGTTTTCTAGCAAATCAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDA1 SAP

Protein Analysis

142

Amino Acids

15.82

Weight (kDa)

8.99

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDA1 PF10172 22 - 78 2.4e-07 Det1 complexing ubiquitin ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 263
AccII CGCG 1 cut(s) 84
AciI CCGC 3 cut(s) 82, 84, 96
AcsI RAATTY 2 cut(s) 337, 374
AfiI CCNNNNNNNGG 1 cut(s) 83
AflII CTTAAG 2 cut(s) 215, 221
AgsI TTSAA 5 cut(s) 68, 115, 255, 369, 374
AluBI AGCT 2 cut(s) 220, 281
AluI AGCT 2 cut(s) 220, 281
Alw26I GTCTC 1 cut(s) 146
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 1 cut(s) 278
ApoI RAATTY 2 cut(s) 337, 374
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 325
AsuC2I CCSGG 1 cut(s) 120
AvaII GGWCC 1 cut(s) 325
BanII GRGCYC 1 cut(s) 290
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 290
BccI CCATC 1 cut(s) 316
BceAI ACGGC 1 cut(s) 7
BciVI GTATCC 1 cut(s) 321
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 1 cut(s) 413
BfoI RGCGCY 1 cut(s) 54
BfrI CTTAAG 2 cut(s) 215, 221
BfuAI ACCTGC 1 cut(s) 263
BfuI GTATCC 1 cut(s) 321
BisI GCNGC 2 cut(s) 85, 279
BlsI GCNGC 2 cut(s) 86, 280
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 325
BmrFI CCNGG 1 cut(s) 120
BmsI GCATC 1 cut(s) 117
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu10I CCTNAGC 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 274
BpuMI CCSGG 1 cut(s) 120
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 249, 307
Bse8I GATNNNNATC 1 cut(s) 33
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 4 cut(s) 132, 175, 265, 327
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 2 cut(s) 32, 273
BseNI ACTGG 2 cut(s) 249, 307
BseXI GCAGC 1 cut(s) 290
BsgI GTGCAG 2 cut(s) 291, 297
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 87
BsiSI CCGG 2 cut(s) 48, 119
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 146
BsnI GGCC 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 290
Bsp143I GATC 2 cut(s) 209, 390
BspACI CCGC 3 cut(s) 82, 84, 96
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 2 cut(s) 31, 274
BspFNI CGCG 1 cut(s) 84
BspMI ACCTGC 1 cut(s) 263
BspTI CTTAAG 2 cut(s) 215, 221
BsrI ACTGG 2 cut(s) 249, 307
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 2 cut(s) 209, 390
Bst4CI ACNGT 1 cut(s) 407
Bst6I CTCTTC 2 cut(s) 19, 95
BstAFI CTTAAG 2 cut(s) 215, 221
BstDEI CTNAG 2 cut(s) 18, 282
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 4 cut(s) 132, 175, 265, 327
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 2 cut(s) 212, 393
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 2 cut(s) 209, 390
BstMWI GCNNNNNNNGC 2 cut(s) 50, 278
BstSCI CCNGG 1 cut(s) 118
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 290
BsuI GTATCC 1 cut(s) 321
BsuRI GGCC 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 4 cut(s) 132, 175, 265, 327
BveI ACCTGC 1 cut(s) 263
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 325
Cfr42I CCGCGG 1 cut(s) 85
CviAII CATG 1 cut(s) 329
CviJI RGCY 7 cut(s) 9, 22, 44, 87, 220, 281, 288
CviKI_1 RGCY 7 cut(s) 9, 22, 44, 87, 220, 281, 288
DdeI CTNAG 2 cut(s) 18, 282
DpnI GATC 2 cut(s) 211, 392
DpnII GATC 2 cut(s) 209, 390
Eam1104I CTCTTC 2 cut(s) 19, 95
EarI CTCTTC 2 cut(s) 19, 95
EciI GGCGGA 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 290
FaeI CATG 1 cut(s) 332
FaiI YATR 7 cut(s) 150, 177, 179, 200, 303, 330, 401
FatI CATG 1 cut(s) 328
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 2 cut(s) 85, 279
FokI GGATG 4 cut(s) 139, 182, 272, 334
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 2 cut(s) 85, 279
FspBI CTAG 1 cut(s) 413
GlaI GCGC 1 cut(s) 52
GluI GCNGC 2 cut(s) 85, 279
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 87
HapII CCGG 2 cut(s) 48, 119
HhaI GCGC 1 cut(s) 53
Hin1II CATG 1 cut(s) 332
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 4 cut(s) 29, 115, 245, 365
HpaII CCGG 2 cut(s) 48, 119
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 2 cut(s) 291, 343
HpyAV CCTTC 4 cut(s) 74, 226, 232, 259
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4V TGCA 2 cut(s) 272, 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 278
HpyF3I CTNAG 2 cut(s) 18, 282
Hsp92II CATG 1 cut(s) 332
HspAI GCGC 1 cut(s) 51
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 2 cut(s) 209, 390
LpnPI CCDG 6 cut(s) 61, 132, 177, 258, 262, 320
Lsp1109I GCAGC 1 cut(s) 290
LweI GCATC 1 cut(s) 117
MaeI CTAG 1 cut(s) 413
MalI GATC 2 cut(s) 211, 392
MboI GATC 2 cut(s) 209, 390
MboII GAAGA 2 cut(s) 6, 82
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 1 cut(s) 290
MluCI AATT 3 cut(s) 337, 369, 374
MnlI CCTC 6 cut(s) 83, 88, 98, 160, 277, 291
MseI TTAA 2 cut(s) 216, 222
MspA1I CMGCKG 2 cut(s) 84, 281
MspCI CTTAAG 2 cut(s) 215, 221
MspI CCGG 2 cut(s) 48, 119
MspR9I CCNGG 1 cut(s) 120
MunI CAATTG 1 cut(s) 369
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 2 cut(s) 50, 278
NciI CCSGG 1 cut(s) 120
NdeII GATC 2 cut(s) 209, 390
NlaIII CATG 1 cut(s) 332
PaqCI CACCTGC 1 cut(s) 263
PfeI GAWTC 4 cut(s) 29, 115, 245, 365
PkrI GCNGC 2 cut(s) 86, 280
PspPI GGNCC 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 281
SacII CCGCGG 1 cut(s) 85
SaqAI TTAA 2 cut(s) 216, 222
SatI GCNGC 2 cut(s) 85, 279
Sau3AI GATC 2 cut(s) 209, 390
Sau96I GGNCC 1 cut(s) 325
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 1 cut(s) 290
SetI ASST 5 cut(s) 75, 222, 277, 283, 302
SfaNI GCATC 1 cut(s) 117
Sfr303I CCGCGG 1 cut(s) 85
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 1 cut(s) 325
SmlI CTYRAG 3 cut(s) 215, 221, 289
SmoI CTYRAG 3 cut(s) 215, 221, 289
Sse9I AATT 3 cut(s) 337, 369, 374
SsiI CCGC 3 cut(s) 82, 84, 96
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 118
TaaI ACNGT 1 cut(s) 407
TasI AATT 3 cut(s) 337, 369, 374
TauI GCSGC 1 cut(s) 87
TfiI GAWTC 4 cut(s) 29, 115, 245, 365
Tru1I TTAA 2 cut(s) 216, 222
Tru9I TTAA 2 cut(s) 216, 222
TseI GCWGC 1 cut(s) 278
TspDTI ATGAA 2 cut(s) 186, 388
Vha464I CTTAAG 2 cut(s) 215, 221
VpaK11BI GGWCC 1 cut(s) 325
XapI RAATTY 2 cut(s) 337, 374
XspI CTAG 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.