Prupe.5G152100_v2.0.a1

Det1 complexing ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0009125
Physical Location & Seq
Forward (+)
13734096 .. 13737190
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G152100.1

Sequence Viewer

Length: 507 bp
ATGGAGGGCTCCTCTTCTCAGCCCTCTGATTCAAATCAGACGGCTTCTGGCGCTTTTAAGTTCCTCCAGAACCTTCCCTCTCGCGGCCTCCTCTCCTCCACCTTCCTCTCATCAAATCCGGGTGGGATGCGTGTGTATGTTTGTGTCCGTGACACATCACCCCCAGAGGATCAACATATACAGACAAACCAACAAAACATATTGATTAGATCACTTACGCTTAAGAAACAGAAGGGAGAATCCAGTTCAAAGGATGTGAAGGGTGCAGGTGCAGCTGAAGGTTCTAGGAAGAGGGTTGCTGAAAGAGTTTTGGATAGCAGAACTTCAGCTAAGAGAGCCGCTAGTCAAAGTAGTTCTCGACAAGAGGGATCAAGCAGCAATCAAGCATCTAGTAGGGACTTCCAGGGGTTAACTGTAGAGAGGCTCCGTGCCCTTCTGAAGGCTAAAGGTCTTTCTTTAAAGGGAAAGAAGGATGAGTTGATTGCACGACTGAAAAGCTCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDA1 SAP

Protein Analysis

169

Amino Acids

18.03

Weight (kDa)

10.4

Isoelectric Point (pI)

58.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 257
AccII CGCG 1 cut(s) 84
AciI CCGC 2 cut(s) 84, 339
AclWI GGATC 2 cut(s) 177, 376
AcuI CTGAAG 3 cut(s) 297, 309, 458
AfiI CCNNNNNNNGG 2 cut(s) 83, 439
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 2 cut(s) 33, 249
AjnI CCWGG 1 cut(s) 402
AluBI AGCT 3 cut(s) 275, 329, 498
AluI AGCT 3 cut(s) 275, 329, 498
AlwI GGATC 2 cut(s) 177, 376
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 2 cut(s) 272, 375
AspLEI GCGC 1 cut(s) 53
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 1 cut(s) 150
BaeGI GKGCMC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 11
BbvI GCAGC 2 cut(s) 284, 387
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 404
BcnI CCSGG 1 cut(s) 120
BfaI CTAG 4 cut(s) 285, 342, 390, 501
BfmI CTRYAG 1 cut(s) 414
BfoI RGCGCY 1 cut(s) 54
BfrI CTTAAG 1 cut(s) 221
BfuAI ACCTGC 1 cut(s) 257
BisI GCNGC 4 cut(s) 85, 273, 339, 376
BlsI GCNGC 4 cut(s) 86, 274, 340, 377
Bme1390I CCNGG 2 cut(s) 120, 404
BmiI GGNNCC 2 cut(s) 10, 425
BmrFI CCNGG 2 cut(s) 120, 404
BmsI GCATC 2 cut(s) 117, 395
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 120
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 403
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 439
Bse1I ACTGG 1 cut(s) 243
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 1 cut(s) 404
BseDI CCNNGG 1 cut(s) 403
BseGI GGATG 3 cut(s) 132, 259, 478
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 2 cut(s) 83, 439
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 243
BseRI GAGGAG 2 cut(s) 80, 85
BseSI GKGCMC 1 cut(s) 433
BseXI GCAGC 2 cut(s) 284, 387
BsgI GTGCAG 2 cut(s) 285, 291
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 87
BsiSI CCGG 1 cut(s) 119
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 2 cut(s) 83, 439
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 1 cut(s) 87
Bsp1286I GDGCHC 2 cut(s) 11, 433
Bsp143I GATC 3 cut(s) 169, 209, 368
BspACI CCGC 2 cut(s) 84, 339
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 1 cut(s) 31
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 10, 425
BspMI ACCTGC 1 cut(s) 257
BspPI GGATC 2 cut(s) 177, 376
BspTI CTTAAG 1 cut(s) 221
BsrI ACTGG 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 3 cut(s) 169, 209, 368
Bst2UI CCWGG 1 cut(s) 404
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 2 cut(s) 19, 284
BstAFI CTTAAG 1 cut(s) 221
BstDEI CTNAG 2 cut(s) 18, 330
BstENI CCTNNNNNAGG 1 cut(s) 437
BstF5I GGATG 3 cut(s) 132, 259, 478
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 3 cut(s) 172, 212, 371
BstMBI GATC 3 cut(s) 169, 209, 368
BstMWI GCNNNNNNNGC 3 cut(s) 50, 272, 335
BstNI CCWGG 1 cut(s) 404
BstSCI CCNGG 2 cut(s) 118, 402
BstSFI CTRYAG 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 433
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 284, 387
BsuRI GGCC 1 cut(s) 87
BtsCI GGATG 3 cut(s) 132, 259, 478
BveI ACCTGC 1 cut(s) 257
CfoI GCGC 1 cut(s) 53
DdeI CTNAG 2 cut(s) 18, 330
DpnI GATC 3 cut(s) 171, 211, 370
DpnII GATC 3 cut(s) 169, 209, 368
DraI TTTAAA 1 cut(s) 459
Eam1104I CTCTTC 2 cut(s) 19, 284
EarI CTCTTC 2 cut(s) 19, 284
Eco24I GRGCYC 1 cut(s) 11
Eco57I CTGAAG 3 cut(s) 297, 309, 458
EcoNI CCTNNNNNAGG 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 11
FaiI YATR 4 cut(s) 138, 177, 179, 200
FaqI GGGAC 1 cut(s) 410
Fnu4HI GCNGC 4 cut(s) 85, 273, 339, 376
FokI GGATG 3 cut(s) 139, 266, 485
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 4 cut(s) 85, 273, 339, 376
FspBI CTAG 4 cut(s) 285, 342, 390, 501
GlaI GCGC 1 cut(s) 52
GluI GCNGC 4 cut(s) 85, 273, 339, 376
GsuI CTGGAG 1 cut(s) 50
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 87
HapII CCGG 1 cut(s) 119
HhaI GCGC 1 cut(s) 53
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HinfI GANTC 2 cut(s) 29, 239
HpaI GTTAAC 1 cut(s) 411
HpaII CCGG 1 cut(s) 119
HphI GGTGA 1 cut(s) 150
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 3 cut(s) 28, 39, 438
Hpy188III TCNNGA 2 cut(s) 67, 357
Hpy8I GTNNAC 1 cut(s) 411
HpyAV CCTTC 8 cut(s) 83, 112, 226, 253, 272, 433, 443, 463
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4V TGCA 3 cut(s) 266, 272, 485
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 272, 335
HpyF3I CTNAG 2 cut(s) 18, 330
HspAI GCGC 1 cut(s) 51
KspAI GTTAAC 1 cut(s) 411
Kzo9I GATC 3 cut(s) 169, 209, 368
LmnI GCTCC 2 cut(s) 14, 429
LpnPI CCDG 8 cut(s) 33, 80, 132, 177, 252, 256, 389, 416
Lsp1109I GCAGC 2 cut(s) 284, 387
LweI GCATC 2 cut(s) 117, 395
MaeI CTAG 4 cut(s) 285, 342, 390, 501
MaeIII GTNAC 1 cut(s) 149
MalI GATC 3 cut(s) 171, 211, 370
MboI GATC 3 cut(s) 169, 209, 368
MboII GAAGA 2 cut(s) 6, 301
MhlI GDGCHC 2 cut(s) 11, 433
MseI TTAA 4 cut(s) 57, 222, 410, 458
MspA1I CMGCKG 1 cut(s) 275
MspCI CTTAAG 1 cut(s) 221
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 2 cut(s) 120, 404
MvaI CCWGG 1 cut(s) 404
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 50, 272, 335
NciI CCSGG 1 cut(s) 120
NdeII GATC 3 cut(s) 169, 209, 368
NlaIV GGNNCC 2 cut(s) 10, 425
NmuCI GTSAC 1 cut(s) 149
PaqCI CACCTGC 1 cut(s) 257
PfeI GAWTC 2 cut(s) 29, 239
PkrI GCNGC 4 cut(s) 86, 274, 340, 377
Psp6I CCWGG 1 cut(s) 402
PspGI CCWGG 1 cut(s) 402
PspN4I GGNNCC 2 cut(s) 10, 425
PvuII CAGCTG 1 cut(s) 275
SaqAI TTAA 4 cut(s) 57, 222, 410, 458
SatI GCNGC 4 cut(s) 85, 273, 339, 376
Sau3AI GATC 3 cut(s) 169, 209, 368
ScrFI CCNGG 2 cut(s) 120, 404
SduI GDGCHC 2 cut(s) 11, 433
SetI ASST 8 cut(s) 75, 104, 271, 277, 283, 331, 451, 500
SfaNI GCATC 2 cut(s) 117, 395
SfcI CTRYAG 1 cut(s) 414
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
SsiI CCGC 2 cut(s) 84, 339
SspMI CTAG 4 cut(s) 285, 342, 390, 501
StyD4I CCNGG 2 cut(s) 118, 402
TaaI ACNGT 1 cut(s) 415
TaqI TCGA 1 cut(s) 358
TauI GCSGC 2 cut(s) 87, 341
TfiI GAWTC 2 cut(s) 29, 239
Tru1I TTAA 4 cut(s) 57, 222, 410, 458
Tru9I TTAA 4 cut(s) 57, 222, 410, 458
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 2 cut(s) 272, 375
Tsp45I GTSAC 1 cut(s) 149
TspGWI ACGGA 2 cut(s) 137, 416
Vha464I CTTAAG 1 cut(s) 221
XagI CCTNNNNNAGG 1 cut(s) 437
XspI CTAG 4 cut(s) 285, 342, 390, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.