Rw7G009510

Det1 complexing ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009125
Physical Location & Seq
Forward (+)
8894363 .. 8897275
2913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009510.1

Sequence Viewer

Length: 405 bp
ATGAATGAAATCCTCTTCCAGGGTAGGATGCGAGTTTGTGTCTGTGAGTACGAGACATCACTCCCAGAGGATGAACATATAAAAACAAACCAACAAAACATATTGATTAGATCACTTAAGCTTAAGAAACAGAAGGAGAAGGGAGATTCCAGTTCAAAGGATGTGAAGGGTGCAGGTGCAGCTGAGGGCTCAAGAAAGAGGGTTGCTGAAAGAGTTTTGGACCGGGCGCCAGCTAAGAAAGCCTCTATGCAACCTCGTCAAGAGGAATCAAGCAATCAAGCATCTAGTAGGGACTTCCAGGGATTAACTGTAGAGAGGCTCCGTGCCCTATTGAAGGCTAAAGGTCTTTCTGTAAAGGGAAAGAAGGAGGAATTGATTGCACGGCTGAGGAGTTCAAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDA1 SAP

Protein Analysis

134

Amino Acids

14.96

Weight (kDa)

9.96

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP PF02037 98 - 130 2.1e-10 SAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 226
AcyI GRCGYC 1 cut(s) 227
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 19, 334
AflII CTTAAG 2 cut(s) 116, 122
AgsI TTSAA 3 cut(s) 156, 334, 396
AjnI CCWGG 2 cut(s) 18, 297
AluBI AGCT 3 cut(s) 121, 182, 233
AluI AGCT 3 cut(s) 121, 182, 233
Alw26I GTCTC 1 cut(s) 47
ApeKI GCWGC 1 cut(s) 179
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 1 cut(s) 220
AsuC2I CCSGG 1 cut(s) 224
AvaII GGWCC 1 cut(s) 220
BaeGI GKGCMC 1 cut(s) 328
BanI GGYRCC 1 cut(s) 226
BanII GRGCYC 1 cut(s) 191
BbvCI CCTCAGC 2 cut(s) 183, 386
BbvI GCAGC 1 cut(s) 191
BceAI ACGGC 1 cut(s) 398
BciT130I CCWGG 2 cut(s) 20, 299
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 309
BfoI RGCGCY 1 cut(s) 230
BfrI CTTAAG 2 cut(s) 116, 122
BfuAI ACCTGC 1 cut(s) 164
BisI GCNGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 181
Bme1390I CCNGG 3 cut(s) 20, 224, 299
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 2 cut(s) 228, 320
BmrFI CCNGG 3 cut(s) 20, 224, 299
BmsI GCATC 2 cut(s) 18, 290
Bpu10I CCTNAGC 2 cut(s) 183, 386
BpuEI CTTGAG 1 cut(s) 175
BpuMI CCSGG 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 19, 298
BsaXI ACNNNNNCTCC 1 cut(s) 382
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 334
Bse1I ACTGG 1 cut(s) 150
BseBI CCWGG 2 cut(s) 20, 299
BseDI CCNNGG 2 cut(s) 19, 298
BseGI GGATG 3 cut(s) 33, 76, 166
BseLI CCNNNNNNNGG 2 cut(s) 19, 334
BseMII CTCAG 2 cut(s) 174, 377
BseNI ACTGG 1 cut(s) 150
BseRI GAGGAG 1 cut(s) 403
BseSI GKGCMC 1 cut(s) 328
BseXI GCAGC 1 cut(s) 191
BsgI GTGCAG 2 cut(s) 192, 198
BshNI GGYRCC 1 cut(s) 226
BsiSI CCGG 1 cut(s) 223
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 2 cut(s) 19, 334
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 191, 328
Bsp143I GATC 1 cut(s) 110
BspCNI CTCAG 2 cut(s) 175, 378
BspLI GGNNCC 2 cut(s) 228, 320
BspMI ACCTGC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 226
BspTI CTTAAG 2 cut(s) 116, 122
BsrI ACTGG 1 cut(s) 150
BssECI CCNNGG 2 cut(s) 19, 298
BssMI GATC 1 cut(s) 110
BssNI GRCGYC 1 cut(s) 227
Bst2UI CCWGG 2 cut(s) 20, 299
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 1 cut(s) 20
BstACI GRCGYC 1 cut(s) 227
BstAFI CTTAAG 2 cut(s) 116, 122
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 3 cut(s) 183, 234, 386
BstENI CCTNNNNNAGG 2 cut(s) 17, 332
BstF5I GGATG 3 cut(s) 33, 76, 166
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 1 cut(s) 113
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 1 cut(s) 110
BstMWI GCNNNNNNNGC 2 cut(s) 179, 239
BstNI CCWGG 2 cut(s) 20, 299
BstSCI CCNGG 3 cut(s) 18, 222, 297
BstSFI CTRYAG 1 cut(s) 309
BstSLI GKGCMC 1 cut(s) 328
BstV1I GCAGC 1 cut(s) 191
BtsCI GGATG 3 cut(s) 33, 76, 166
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 1 cut(s) 231
CfoI GCGC 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 220
Csp6I GTAC 1 cut(s) 49
CviJI RGCY 8 cut(s) 121, 182, 189, 233, 242, 319, 338, 385
CviKI_1 RGCY 8 cut(s) 121, 182, 189, 233, 242, 319, 338, 385
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 3 cut(s) 183, 234, 386
DinI GGCGCC 1 cut(s) 228
DpnI GATC 1 cut(s) 112
DpnII GATC 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 220
EcoNI CCTNNNNNAGG 2 cut(s) 17, 332
EcoRII CCWGG 2 cut(s) 18, 297
EcoT38I GRGCYC 1 cut(s) 191
EgeI GGCGCC 1 cut(s) 228
EheI GGCGCC 1 cut(s) 228
FaiI YATR 4 cut(s) 78, 80, 101, 248
FaqI GGGAC 1 cut(s) 305
Fnu4HI GCNGC 1 cut(s) 180
FokI GGATG 3 cut(s) 40, 83, 173
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 180
FspBI CTAG 1 cut(s) 285
GlaI GCGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 180
HaeII RGCGCY 1 cut(s) 230
HapII CCGG 1 cut(s) 223
HhaI GCGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 227
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 2 cut(s) 146, 266
HpaII CCGG 1 cut(s) 223
Hpy188III TCNNGA 2 cut(s) 192, 260
HpyAV CCTTC 5 cut(s) 127, 133, 160, 328, 358
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4V TGCA 4 cut(s) 173, 179, 250, 380
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 239
HpyF3I CTNAG 3 cut(s) 183, 234, 386
Hsp92I GRCGYC 1 cut(s) 227
HspAI GCGC 1 cut(s) 227
KasI GGCGCC 1 cut(s) 226
Kzo9I GATC 1 cut(s) 110
LmnI GCTCC 1 cut(s) 324
LpnPI CCDG 9 cut(s) 5, 32, 78, 159, 163, 236, 243, 284, 311
Lsp1109I GCAGC 1 cut(s) 191
LweI GCATC 2 cut(s) 18, 290
MaeI CTAG 1 cut(s) 285
MalI GATC 1 cut(s) 112
MboI GATC 1 cut(s) 110
MboII GAAGA 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 191, 328
MluCI AATT 1 cut(s) 371
Mly113I GGCGCC 1 cut(s) 227
MseI TTAA 3 cut(s) 117, 123, 305
MspA1I CMGCKG 1 cut(s) 182
MspCI CTTAAG 2 cut(s) 116, 122
MspI CCGG 1 cut(s) 223
MspR9I CCNGG 3 cut(s) 20, 224, 299
MvaI CCWGG 2 cut(s) 20, 299
MwoI GCNNNNNNNGC 2 cut(s) 179, 239
NarI GGCGCC 1 cut(s) 227
NciI CCSGG 1 cut(s) 224
NdeII GATC 1 cut(s) 110
NlaIV GGNNCC 2 cut(s) 228, 320
PaqCI CACCTGC 1 cut(s) 164
PfeI GAWTC 2 cut(s) 146, 266
PkrI GCNGC 1 cut(s) 181
PluTI GGCGCC 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 18, 297
PspGI CCWGG 2 cut(s) 18, 297
PspN4I GGNNCC 2 cut(s) 228, 320
PspPI GGNCC 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SaqAI TTAA 3 cut(s) 117, 123, 305
SatI GCNGC 1 cut(s) 180
Sau3AI GATC 1 cut(s) 110
Sau96I GGNCC 1 cut(s) 220
ScrFI CCNGG 3 cut(s) 20, 224, 299
SduI GDGCHC 2 cut(s) 191, 328
SetI ASST 6 cut(s) 123, 178, 184, 235, 256, 346
SfaNI GCATC 2 cut(s) 18, 290
SfcI CTRYAG 1 cut(s) 309
SfoI GGCGCC 1 cut(s) 228
SinI GGWCC 1 cut(s) 220
SmlI CTYRAG 3 cut(s) 116, 122, 190
SmoI CTYRAG 3 cut(s) 116, 122, 190
Sse9I AATT 1 cut(s) 371
SspDI GGCGCC 1 cut(s) 226
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 3 cut(s) 18, 222, 297
TaaI ACNGT 1 cut(s) 310
TasI AATT 1 cut(s) 371
TfiI GAWTC 2 cut(s) 146, 266
Tru1I TTAA 3 cut(s) 117, 123, 305
Tru9I TTAA 3 cut(s) 117, 123, 305
TseI GCWGC 1 cut(s) 179
TspDTI ATGAA 3 cut(s) 17, 21, 87
TspGWI ACGGA 1 cut(s) 311
Vha464I CTTAAG 2 cut(s) 116, 122
VpaK11BI GGWCC 1 cut(s) 220
XagI CCTNNNNNAGG 2 cut(s) 17, 332
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.