FvH4_5g10010

Respiratory burst oxidase homolog protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004577
Physical Location & Seq
Reverse (-)
5701622 .. 5708041
6420 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10010.t2

Sequence Viewer

Length: 2754 bp
ATGAAGGAAACAGCCCCAGAAGATCATATCGGAGAAGCTGCTACTTCCGCCGGAGTAGCACCACCACTTCAGTATCACTCCGACAACGAGCTCCCCGCACCCGAGAAAATGTCCGGCGCCGGCTACACCGCTCCGCTCAGTCTCAGCGGGACTCTGAACAAAAGAACCGGCAGCAAGAGAACCAACAACCACGAAGAAGAGCCTTACGTCGAGGACGTCTCCGTCGACGTAAGCAGCGTCAAGCCCCTGCCACGTGGCGACGCCAACAACACAGACGACTCCCTCACTCTTCTCGGCGGCGACAAGCCGCCCCAGAAGAGCAACAACGACCAGTCGTTCGTACGTACAGCCTCCGTACGGATCAAGCAGGAGCTGAAGAAGCTGACGTCGTTCTCGAAGCAGCAGCTGCCGCAACGGCGCCGTTTCGACCGGATGCAGTCAGCGGCTGCTCCGGCTCTTAAGGGACTCAAGTTCATAAGTAAAACGGACGGGAATGCTGAGTGGCCGGCGGTCGAGAAAAGGTTCGACAAGCTTACAGGCTCGACTAACGGGATGCTGCCACGTGCTGCGTTCGGGGAGTGCATAGGGATGAACAAAGACTGCAAGGAGTTCGCCGGAAAACTGTTCGACGCTTTGGCCAGGAAGCATGGGATCAAAGGCGATTCGATCAACAAGGAGGAGTTGAAAGAGATGTGGACACAAGTTTCTGAGCAGGGCTTCAATTCCAGGCTGCAAATTTTCTTTGACATGGTTGATAAGGATGGGGATGGAAGAATCAGTGTGGAAGAAGTCAAGGAGATCATTAGCTTCAGTGCTTCTGCCAATAAACTATCAAACATCCAGACACAAGCACAGGAATATGCAGCATTGATCATGGAAGAGTTGGATCCAGACAATCTTGGATACATCATGATAGACAGCTTGGAGACACTCCTACTGTATGGACCGGAGGAGACGGTGGCGCGTGGAAAAGAGAGCCGGAACTTGAGCAAAATGCTGAGCCAGAAGCTGAAGCCAGTGAGGGAGGACAACCCTCTGACAAGGTGGTATAGGAGAGCCAACTACTTTCTTGAAGACAATTGGAAGAGAGTTTGGGTAATGGCATTGTGGATTGGGATCATGGCTGCTCTGTTTGCCTATAAGTATGTGCAGTATAAAAATAGGGCTGCATATCACGTAATGGGTCATTGTGTATCCATGGCCAAAGGTGCAGCTGAGACACTCAAGTTCAACATGGCTCTTATTCTACTTCCAGTGTGTAGAAACACTATCACTTTGCTAAGGAACAAGACCAAATTGGGTGTTGCCGTTCCTTTTGATGATAACCTCAATTTCCATAAGGTGATAGCTATTGGAATTTCAGTAGGAGTTGGCATACACTCGATTTACCATTTGGCGTGCGATTTCCCTCGTCTCATCAAAGCAGACAGCGTCACATACGCCCCGATGAGGCAATATTTTGGGGAACAACCTTCAAACTATTGGTTTTTTGTGAAATCAGTAGAAGGTGTAACTGGCATTATAATGGTAGTGTTGATGACCATAGCCTTCACACTAGCAGCACCATGTTTTAGAAGAGGTAAGCTCAAAAACCTACCCCAACCTCTCAAAAGGCTCACTGGCTTCAATGCCTTTTGGTACTCCCATCATCTCTTCGTCATTGTCTATACCTTGCTCATTGTCCATGGAGAATACCTTTACCTCACCAAGGAATGGTACAAGAAAACGACTTGGATGTACTTGGCTGTTCCACTTCTCCTCTACGGTGGTGAAAGATTGGTTCGGGCACTCCGGTCCAGCATCAAGCCGGTTAAGATAGTAAAGGTGGCTCTATATCCTGGAAATGTGTTGGCTCTTTTTATGTCAAAGCCTCAGGGCTTCAGATACCATAGTGGGCAATACATGTTTGTTAATTGTGCTGCTGTCTCCCCATTTGAATGGCACCCATTTTCGATCACGTCGGCGCCGGGAGACGACCACCTCAGTGTTCATATCCGGACTCTTGGCGACTGGACGCGACAACTTAAGACGGTGTTTTCTCAGGTATGCCAACCACCTCCTTCTGGGAACAGTGGTCTCCTCAGAGCCGACTGTATGCAAAGCAACGACTTAAACTTTCCAAGAGTGCTAATAGATGGACCCTATGGAGCACCAGCTCAAGACTACAAGAACTATGAAGTGGTGTTACTTGTAGGGTTGGGAATAGGAGCAACCCCAATGATAAGCATCCTCAAGGACATAGTGAACAACATCAAAGCCATAGAAGATGAATCACAGAACGCCCTAGAAAGCGGCACGGCCGGTTCTGGCAGCCCAGTGAGCCTCCACAGCAACTTCAAGACCAAGAGAGCCTACTTCTACTGGGTGACGCGAGAGCAAGGTTCGTTCGACTGGTTCAAAGGAGCCATGAACGAGGTGGCGGAGCTTGACCATAACAGAGTGATAGAGTTCCACAACTATTGCACTAGTGTTTATGAGGAAGGTGATGCACGTTCTGCCCTCATTGCTATGCTTCAGTCACTAAATCATGCCAAGAATGGGGTCGATATTGTGTCGGGTACTCGGGTGAAGTCACATTTTGCTAAGCCTAACTGGCGTGAGGTCTACAAGCGCATTGCGCTGGATCACACCAATGACAAAGTTGGGGTGTTCTACTGCGGGCCACCTGCTCTAACTGAAGAACTACGACAGCTATCTCTGGATTTCAACCACAAAACCTCCACCAGATTTGAATTCCATAAAGAAAATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

918

Amino Acids

103.18

Weight (kDa)

8.94

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 141 - 242 8.6e-35 Respiratory burst NADPH oxidase
EF-hand_7 PF13499 242 - 303 7.4e-06 EF-hand domain pair
Ferric_reduct PF01794 412 - 559 1.7e-11 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 603 - 719 2.8e-35 FAD-binding domain
NAD_binding_6 PF08030 725 - 899 1.2e-50 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1523
AarI CACCTGC 1 cut(s) 2674
AasI GACNNNNNNGTC 1 cut(s) 221
AatII GACGTC 2 cut(s) 219, 389
Acc36I ACCTGC 1 cut(s) 2674
AccB1I GGYRCC 4 cut(s) 116, 417, 1941, 1963
AccB7I CCANNNNNTGG 1 cut(s) 1713
AccBSI CCGCTC 2 cut(s) 131, 136
AccI GTMKAC 2 cut(s) 225, 2604
AccII CGCG 3 cut(s) 964, 2017, 2371
AccIII TCCGGA 1 cut(s) 1995
AclWI GGATC 6 cut(s) 368, 659, 881, 894, 1124, 2631
AcoI YGGCCR 4 cut(s) 503, 636, 1200, 2298
AcsI RAATTY 4 cut(s) 735, 1356, 2732, 2746
AcuI CTGAAG 7 cut(s) 53, 395, 793, 1031, 1864, 2498, 2697
AcvI CACGTG 2 cut(s) 254, 563
AcyI GRCGYC 6 cut(s) 117, 216, 261, 386, 418, 1964
AfaI GTAC 7 cut(s) 342, 346, 357, 1640, 1718, 1739, 2560
AfiI CCNNNNNNNGG 6 cut(s) 357, 1449, 1708, 1713, 2063, 2538
AflII CTTAAG 2 cut(s) 458, 2024
AflIII ACRYGT 1 cut(s) 1902
AhlI ACTAGT 1 cut(s) 2465
AjiI CACGTC 1 cut(s) 1959
AjnI CCWGG 3 cut(s) 638, 725, 1837
AleI CACNNNNGTG 1 cut(s) 1983
Alw21I GWGCWC 2 cut(s) 93, 2152
Alw26I GTCTC 9 cut(s) 146, 223, 920, 947, 1211, 1418, 1930, 1965, 2081
AlwI GGATC 6 cut(s) 368, 659, 881, 894, 1124, 2631
AlwNI CAGNNNCTG 4 cut(s) 373, 406, 446, 1009
Ama87I CYCGRG 2 cut(s) 101, 2562
Aor13HI TCCGGA 1 cut(s) 1995
AoxI GGCC 5 cut(s) 503, 636, 1200, 2298, 2660
ApoI RAATTY 4 cut(s) 735, 1356, 2732, 2746
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
Asp700I GAANNNNTTC 1 cut(s) 521
AspLEI GCGC 6 cut(s) 119, 420, 964, 1966, 2613, 2620
AspS9I GGNCC 4 cut(s) 944, 1794, 2138, 2660
AsuC2I CCSGG 1 cut(s) 1968
AsuHPI GGTGA 6 cut(s) 1354, 1696, 1781, 2377, 2495, 2578
AvaI CYCGRG 2 cut(s) 101, 2562
AvaII GGWCC 3 cut(s) 944, 1794, 2138
AxyI CCTNAGG 1 cut(s) 1872
BaeGI GKGCMC 1 cut(s) 1789
BaeI ACNNNNGTAYC 6 cut(s) 56, 89, 1876, 1909, 2550, 2583
BalI TGGCCA 2 cut(s) 638, 1202
BamHI GGATCC 1 cut(s) 886
BanI GGYRCC 4 cut(s) 116, 417, 1941, 1963
BanII GRGCYC 1 cut(s) 93
BarI GAAGNNNNNNTAC 2 cut(s) 2169, 2201
BbrPI CACGTG 2 cut(s) 254, 563
BbsI GAAGAC 1 cut(s) 1080
Bbv12I GWGCWC 2 cut(s) 93, 2152
BccI CCATC 4 cut(s) 755, 761, 1653, 2129
BceAI ACGGC 4 cut(s) 405, 431, 1292, 2313
BcgI CGANNNNNNTGC 2 cut(s) 592, 626
BciT130I CCWGG 3 cut(s) 640, 727, 1839
BciVI GTATCC 2 cut(s) 896, 1204
BclI TGATCA 1 cut(s) 870
BcnI CCSGG 1 cut(s) 1968
BcoDI GTCTC 9 cut(s) 146, 223, 920, 947, 1211, 1418, 1930, 1965, 2081
BcuI ACTAGT 1 cut(s) 2465
BfaI CTAG 3 cut(s) 1556, 2285, 2466
BfoI RGCGCY 3 cut(s) 120, 421, 1967
BfrI CTTAAG 2 cut(s) 458, 2024
BfuAI ACCTGC 1 cut(s) 2674
BfuI GTATCC 2 cut(s) 896, 1204
BglI GCCNNNNNGGC 2 cut(s) 415, 2593
BlpI GCTNAGC 2 cut(s) 998, 2583
Bme1390I CCNGG 4 cut(s) 640, 727, 1839, 1968
Bme18I GGWCC 3 cut(s) 944, 1794, 2138
BmeT110I CYCGRG 2 cut(s) 101, 2562
BmgBI CACGTC 1 cut(s) 1959
BmgT120I GGNCC 4 cut(s) 944, 1794, 2138, 2660
BmiI GGNNCC 7 cut(s) 118, 419, 888, 1943, 1965, 2140, 2404
BmrFI CCNGG 4 cut(s) 640, 727, 1839, 1968
BmrI ACTGGG 2 cut(s) 2309, 2371
BmsI GCATC 5 cut(s) 423, 543, 1809, 2235, 2476
BmuI ACTGGG 2 cut(s) 2309, 2371
BpiI GAAGAC 1 cut(s) 1080
BplI GAGNNNNNCTC 4 cut(s) 203, 235, 1569, 1601
Bpu10I CCTNAGC 1 cut(s) 1280
Bpu1102I GCTNAGC 2 cut(s) 998, 2583
BpuEI CTTGAG 5 cut(s) 452, 1006, 1208, 2142, 2216
BpuMI CCSGG 1 cut(s) 1968
BsaAI YACGTR 4 cut(s) 254, 344, 563, 1177
BsaBI GATNNNNATC 2 cut(s) 1115, 2225
BsaHI GRCGYC 6 cut(s) 117, 216, 261, 386, 418, 1964
BsaI GGTCTC 1 cut(s) 2081
BsaJI CCNNGG 3 cut(s) 1197, 1684, 1707
BsaWI WCCGGW 4 cut(s) 429, 946, 1791, 1995
BsaXI ACNNNNNCTCC 2 cut(s) 2702, 2732
Bsc4I CCNNNNNNNGG 6 cut(s) 357, 1449, 1708, 1713, 2063, 2538
Bse118I RCCGGY 5 cut(s) 119, 167, 505, 1807, 2300
Bse21I CCTNAGG 1 cut(s) 1872
Bse3DI GCAATG 2 cut(s) 2502, 2613
Bse8I GATNNNNATC 2 cut(s) 1115, 2225
BseAI TCCGGA 1 cut(s) 1995
BseBI CCWGG 3 cut(s) 640, 727, 1839
BseDI CCNNGG 3 cut(s) 1197, 1684, 1707
BseGI GGATG 8 cut(s) 438, 558, 594, 766, 772, 837, 1740, 2226
BseJI GATNNNNATC 2 cut(s) 1115, 2225
BseLI CCNNNNNNNGG 6 cut(s) 357, 1449, 1708, 1713, 2063, 2538
BseMI GCAATG 2 cut(s) 2502, 2613
BseRI GAGGAG 4 cut(s) 692, 965, 1748, 2069
BseSI GKGCMC 1 cut(s) 1789
BseX3I CGGCCG 1 cut(s) 2298
BsgI GTGCAG 2 cut(s) 1169, 1230
Bsh1236I CGCG 3 cut(s) 964, 2017, 2371
Bsh1285I CGRYCG 3 cut(s) 430, 513, 2301
BshFI GGCC 5 cut(s) 505, 638, 1202, 2300, 2662
BshNI GGYRCC 4 cut(s) 116, 417, 1941, 1963
BsiEI CGRYCG 3 cut(s) 430, 513, 2301
BsiHKAI GWGCWC 2 cut(s) 93, 2152
BsiHKCI CYCGRG 2 cut(s) 101, 2562
BsiWI CGTACG 2 cut(s) 340, 355
BslFI GGGAC 2 cut(s) 163, 477
BslI CCNNNNNNNGG 6 cut(s) 357, 1449, 1708, 1713, 2063, 2538
BsmAI GTCTC 9 cut(s) 146, 223, 920, 947, 1211, 1418, 1930, 1965, 2081
BsmBI CGTCTC 4 cut(s) 223, 947, 1418, 1965
BsmFI GGGAC 2 cut(s) 163, 477
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 5 cut(s) 505, 638, 1202, 2300, 2662
Bso31I GGTCTC 1 cut(s) 2081
BsoBI CYCGRG 2 cut(s) 101, 2562
Bsp1286I GDGCHC 3 cut(s) 93, 1789, 2152
Bsp13I TCCGGA 1 cut(s) 1995
Bsp1720I GCTNAGC 2 cut(s) 998, 2583
Bsp19I CCATGG 2 cut(s) 1197, 1684
BspANI GGCC 5 cut(s) 505, 638, 1202, 2300, 2662
BspEI TCCGGA 1 cut(s) 1995
BspFNI CGCG 3 cut(s) 964, 2017, 2371
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 7 cut(s) 118, 419, 888, 1943, 1965, 2140, 2404
BspMI ACCTGC 1 cut(s) 2674
BspPI GGATC 6 cut(s) 368, 659, 881, 894, 1124, 2631
BspQI GCTCTTC 2 cut(s) 192, 311
BspT107I GGYRCC 4 cut(s) 116, 417, 1941, 1963
BspTI CTTAAG 2 cut(s) 458, 2024
BspTNI GGTCTC 1 cut(s) 2081
BsrBI CCGCTC 2 cut(s) 131, 136
BsrDI GCAATG 2 cut(s) 2502, 2613
BsrFI RCCGGY 5 cut(s) 119, 167, 505, 1807, 2300
BssAI RCCGGY 5 cut(s) 119, 167, 505, 1807, 2300
BssECI CCNNGG 3 cut(s) 1197, 1684, 1707
BssNI GRCGYC 6 cut(s) 117, 216, 261, 386, 418, 1964
BssT1I CCWWGG 3 cut(s) 1197, 1684, 1707
Bst2UI CCWGG 3 cut(s) 640, 727, 1839
Bst4CI ACNGT 7 cut(s) 624, 939, 958, 1766, 2032, 2072, 2093
Bst6I CTCTTC 7 cut(s) 192, 294, 311, 873, 1079, 1570, 1658
BstACI GRCGYC 6 cut(s) 117, 216, 261, 386, 418, 1964
BstAFI CTTAAG 2 cut(s) 458, 2024
BstAPI GCANNNNNTGC 2 cut(s) 406, 2495
BstBAI YACGTR 4 cut(s) 254, 344, 563, 1177
BstC8I GCNNGC 4 cut(s) 121, 507, 1399, 2660
BstDSI CCRYGG 2 cut(s) 1197, 1684
BstENI CCTNNNNNAGG 1 cut(s) 1706
BstF5I GGATG 8 cut(s) 438, 558, 594, 766, 772, 837, 1740, 2226
BstFNI CGCG 3 cut(s) 964, 2017, 2371
BstH2I RGCGCY 3 cut(s) 120, 421, 1967
BstHHI GCGC 6 cut(s) 119, 420, 964, 1966, 2613, 2620
BstMAI GTCTC 9 cut(s) 146, 223, 920, 947, 1211, 1418, 1930, 1965, 2081
BstMCI CGRYCG 3 cut(s) 430, 513, 2301
BstNI CCWGG 3 cut(s) 640, 727, 1839
BstNSI RCATGY 1 cut(s) 1906
BstSCI CCNGG 4 cut(s) 638, 725, 1837, 1966
BstSLI GKGCMC 1 cut(s) 1789
BstSNI TACGTA 1 cut(s) 344
BstUI CGCG 3 cut(s) 964, 2017, 2371
BstV2I GAAGAC 1 cut(s) 1080
BstX2I RGATCY 1 cut(s) 886
BstXI CCANNNNNNTGG 1 cut(s) 1938
BstYI RGATCY 1 cut(s) 886
BstZI CGGCCG 1 cut(s) 2298
Bsu36I CCTNAGG 1 cut(s) 1872
BsuI GTATCC 2 cut(s) 896, 1204
BsuRI GGCC 5 cut(s) 505, 638, 1202, 2300, 2662
BtgI CCRYGG 2 cut(s) 1197, 1684
BtrI CACGTC 1 cut(s) 1959
BtsCI GGATG 8 cut(s) 438, 558, 594, 766, 772, 837, 1740, 2226
BtsIMutI CAGTG 8 cut(s) 784, 817, 1023, 1260, 1617, 1990, 2077, 2322
BveI ACCTGC 1 cut(s) 2674
Cac8I GCNNGC 4 cut(s) 121, 507, 1399, 2660
CaiI CAGNNNCTG 4 cut(s) 373, 406, 446, 1009
CciI TCATGA 1 cut(s) 909
CfoI GCGC 6 cut(s) 119, 420, 964, 1966, 2613, 2620
Cfr10I RCCGGY 5 cut(s) 119, 167, 505, 1807, 2300
Cfr13I GGNCC 4 cut(s) 944, 1794, 2138, 2660
CseI GACGC 6 cut(s) 226, 269, 638, 1420, 2023, 2377
Csp6I GTAC 7 cut(s) 341, 345, 356, 1639, 1717, 1738, 2559
CspCI CAANNNNNGTGG 4 cut(s) 2441, 2476, 2710, 2745
CviQI GTAC 7 cut(s) 341, 345, 356, 1639, 1717, 1738, 2559
DinI GGCGCC 3 cut(s) 118, 419, 1965
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 4 cut(s) 503, 636, 1200, 2298
EagI CGGCCG 1 cut(s) 2298
Eam1104I CTCTTC 7 cut(s) 192, 294, 311, 873, 1079, 1570, 1658
EarI CTCTTC 7 cut(s) 192, 294, 311, 873, 1079, 1570, 1658
EciI GGCGGA 2 cut(s) 37, 2435
Ecl136II GAGCTC 1 cut(s) 91
EclXI CGGCCG 1 cut(s) 2298
Eco105I TACGTA 1 cut(s) 344
Eco130I CCWWGG 3 cut(s) 1197, 1684, 1707
Eco24I GRGCYC 1 cut(s) 93
Eco31I GGTCTC 1 cut(s) 2081
Eco47I GGWCC 3 cut(s) 944, 1794, 2138
Eco52I CGGCCG 1 cut(s) 2298
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 7 cut(s) 53, 395, 793, 1031, 1864, 2498, 2697
Eco72I CACGTG 2 cut(s) 254, 563
Eco81I CCTNAGG 1 cut(s) 1872
Eco88I CYCGRG 2 cut(s) 101, 2562
EcoICRI GAGCTC 1 cut(s) 91
EcoNI CCTNNNNNAGG 1 cut(s) 1706
EcoRI GAATTC 1 cut(s) 2732
EcoRII CCWGG 3 cut(s) 638, 725, 1837
EcoT14I CCWWGG 3 cut(s) 1197, 1684, 1707
EcoT38I GRGCYC 1 cut(s) 93
EgeI GGCGCC 3 cut(s) 118, 419, 1965
EheI GGCGCC 3 cut(s) 118, 419, 1965
ErhI CCWWGG 3 cut(s) 1197, 1684, 1707
Esp3I CGTCTC 4 cut(s) 223, 947, 1418, 1965
FaqI GGGAC 2 cut(s) 163, 477
FauI CCCGC 3 cut(s) 103, 140, 2651
FbaI TGATCA 1 cut(s) 870
FblI GTMKAC 2 cut(s) 225, 2604
FokI GGATG 8 cut(s) 445, 565, 601, 773, 779, 824, 1747, 2213
FriOI GRGCYC 1 cut(s) 93
FspBI CTAG 3 cut(s) 1556, 2285, 2466
GlaI GCGC 6 cut(s) 118, 419, 963, 1965, 2612, 2619
HaeII RGCGCY 3 cut(s) 120, 421, 1967
HaeIII GGCC 5 cut(s) 505, 638, 1202, 2300, 2662
HgaI GACGC 6 cut(s) 226, 269, 638, 1420, 2023, 2377
HhaI GCGC 6 cut(s) 119, 420, 964, 1966, 2613, 2620
Hin1I GRCGYC 6 cut(s) 117, 216, 261, 386, 418, 1964
Hin6I GCGC 6 cut(s) 117, 418, 962, 1964, 2611, 2618
HinP1I GCGC 6 cut(s) 117, 418, 962, 1964, 2611, 2618
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 7 cut(s) 151, 278, 465, 662, 774, 1999, 2270
HphI GGTGA 6 cut(s) 1354, 1696, 1781, 2377, 2495, 2578
Hpy166II GTNNAC 4 cut(s) 226, 696, 2245, 2605
Hpy188I TCNGA 8 cut(s) 32, 82, 156, 709, 1038, 1883, 2084, 2753
Hpy8I GTNNAC 4 cut(s) 226, 696, 2245, 2605
Hpy99I CGWCG 7 cut(s) 212, 227, 230, 263, 391, 632, 1963
HpyAV CCTTC 5 cut(s) 1482, 1499, 1558, 2070, 2474
HpyCH4III ACNGT 7 cut(s) 624, 939, 958, 1766, 2032, 2072, 2093
Hsp92I GRCGYC 6 cut(s) 117, 216, 261, 386, 418, 1964
HspAI GCGC 6 cut(s) 117, 418, 962, 1964, 2611, 2618
KasI GGCGCC 3 cut(s) 116, 417, 1963
Kpn2I TCCGGA 1 cut(s) 1995
KroI GCCGGC 2 cut(s) 119, 505
KroNI GCCGGC 2 cut(s) 121, 507
Ksp22I TGATCA 1 cut(s) 870
LguI GCTCTTC 2 cut(s) 192, 311
LmnI GCTCC 8 cut(s) 96, 136, 370, 454, 2147, 2207, 2402, 2422
LweI GCATC 5 cut(s) 423, 543, 1809, 2235, 2476
MaeI CTAG 3 cut(s) 1556, 2285, 2466
MaeIII GTNAC 6 cut(s) 1432, 1510, 2184, 2365, 2517, 2571
MbiI CCGCTC 2 cut(s) 131, 136
MfeI CAATTG 1 cut(s) 1078
MflI RGATCY 1 cut(s) 886
MhlI GDGCHC 3 cut(s) 93, 1789, 2152
MlsI TGGCCA 2 cut(s) 638, 1202
MluCI AATT 9 cut(s) 721, 735, 1078, 1295, 1330, 1356, 1912, 2732, 2746
MluNI TGGCCA 2 cut(s) 638, 1202
Mly113I GGCGCC 3 cut(s) 117, 418, 1964
MlyI GAGTC 4 cut(s) 145, 272, 459, 1993
MmeI TCCRAC 2 cut(s) 105, 864
Mox20I TGGCCA 2 cut(s) 638, 1202
MroI TCCGGA 1 cut(s) 1995
MroNI GCCGGC 2 cut(s) 119, 505
MroXI GAANNNNTTC 1 cut(s) 521
MscI TGGCCA 2 cut(s) 638, 1202
MseI TTAA 5 cut(s) 459, 1812, 1911, 2025, 2111
MslI CAYNNNNRTG 6 cut(s) 587, 1523, 1936, 1983, 2507, 2631
Msp20I TGGCCA 2 cut(s) 638, 1202
MspA1I CMGCKG 4 cut(s) 147, 406, 443, 1214
MspCI CTTAAG 2 cut(s) 458, 2024
MspR9I CCNGG 4 cut(s) 640, 727, 1839, 1968
MunI CAATTG 1 cut(s) 1078
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 3 cut(s) 640, 727, 1839
MvnI CGCG 3 cut(s) 964, 2017, 2371
NaeI GCCGGC 2 cut(s) 121, 507
NarI GGCGCC 3 cut(s) 117, 418, 1964
NciI CCSGG 1 cut(s) 1968
NcoI CCATGG 2 cut(s) 1197, 1684
NgoMIV GCCGGC 2 cut(s) 119, 505
NlaIV GGNNCC 7 cut(s) 118, 419, 888, 1943, 1965, 2140, 2404
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 4 cut(s) 1432, 2365, 2517, 2571
NspI RCATGY 1 cut(s) 1906
OliI CACNNNNGTG 1 cut(s) 1983
PagI TCATGA 1 cut(s) 909
PaqCI CACCTGC 1 cut(s) 2674
PciI ACATGT 1 cut(s) 1902
PciSI GCTCTTC 2 cut(s) 192, 311
PcsI WCGNNNNNNNCGW 4 cut(s) 213, 222, 234, 392
PctI GAATGC 1 cut(s) 499
PdiI GCCGGC 2 cut(s) 121, 507
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 3 cut(s) 662, 774, 2270
Pfl23II CGTACG 2 cut(s) 340, 355
PflFI GACNNNGTC 1 cut(s) 1430
PflMI CCANNNNNTGG 1 cut(s) 1713
PfoI TCCNGGA 1 cut(s) 1837
PleI GAGTC 4 cut(s) 145, 272, 459, 1993
PluTI GGCGCC 3 cut(s) 120, 421, 1967
PmaCI CACGTG 2 cut(s) 254, 563
PmlI CACGTG 2 cut(s) 254, 563
PpsI GAGTC 4 cut(s) 145, 272, 459, 1993
Ppu21I YACGTR 4 cut(s) 254, 344, 563, 1177
PscI ACATGT 1 cut(s) 1902
PsiI TTATAA 1 cut(s) 1523
Psp124BI GAGCTC 1 cut(s) 93
Psp6I CCWGG 3 cut(s) 638, 725, 1837
PspCI CACGTG 2 cut(s) 254, 563
PspGI CCWGG 3 cut(s) 638, 725, 1837
PspLI CGTACG 2 cut(s) 340, 355
PspN4I GGNNCC 7 cut(s) 118, 419, 888, 1943, 1965, 2140, 2404
PspPI GGNCC 4 cut(s) 944, 1794, 2138, 2660
PstNI CAGNNNCTG 4 cut(s) 373, 406, 446, 1009
PsuI RGATCY 1 cut(s) 886
PsyI GACNNNGTC 1 cut(s) 1430
PvuII CAGCTG 2 cut(s) 406, 1214
RsaI GTAC 7 cut(s) 342, 346, 357, 1640, 1718, 1739, 2560
RsaNI GTAC 7 cut(s) 341, 345, 356, 1639, 1717, 1738, 2559
RseI CAYNNNNRTG 6 cut(s) 587, 1523, 1936, 1983, 2507, 2631
SacI GAGCTC 1 cut(s) 93
SalI GTCGAC 1 cut(s) 224
SapI GCTCTTC 2 cut(s) 192, 311
SaqAI TTAA 5 cut(s) 459, 1812, 1911, 2025, 2111
Sau96I GGNCC 4 cut(s) 944, 1794, 2138, 2660
SchI GAGTC 4 cut(s) 145, 272, 459, 1993
ScrFI CCNGG 4 cut(s) 640, 727, 1839, 1968
SduI GDGCHC 3 cut(s) 93, 1789, 2152
SfaNI GCATC 5 cut(s) 423, 543, 1809, 2235, 2476
SfoI GGCGCC 3 cut(s) 118, 419, 1965
SgrDI CGTCGACG 1 cut(s) 224
SinI GGWCC 3 cut(s) 944, 1794, 2138
SmiMI CAYNNNNRTG 6 cut(s) 587, 1523, 1936, 1983, 2507, 2631
SmlI CTYRAG 7 cut(s) 458, 467, 985, 1223, 2024, 2157, 2231
SmoI CTYRAG 7 cut(s) 458, 467, 985, 1223, 2024, 2157, 2231
SnaBI TACGTA 1 cut(s) 344
SpeI ACTAGT 1 cut(s) 2465
Sse9I AATT 9 cut(s) 721, 735, 1078, 1295, 1330, 1356, 1912, 2732, 2746
SspDI GGCGCC 3 cut(s) 116, 417, 1963
SspI AATATT 1 cut(s) 1457
SspMI CTAG 3 cut(s) 1556, 2285, 2466
SstI GAGCTC 1 cut(s) 93
StyD4I CCNGG 4 cut(s) 638, 725, 1837, 1966
StyI CCWWGG 3 cut(s) 1197, 1684, 1707
TaaI ACNGT 7 cut(s) 624, 939, 958, 1766, 2032, 2072, 2093
TasI AATT 9 cut(s) 721, 735, 1078, 1295, 1330, 1356, 1912, 2732, 2746
TatI WGTACW 1 cut(s) 1737
TauI GCSGC 5 cut(s) 300, 310, 412, 446, 2295
TfiI GAWTC 3 cut(s) 662, 774, 2270
Tru1I TTAA 5 cut(s) 459, 1812, 1911, 2025, 2111
Tru9I TTAA 5 cut(s) 459, 1812, 1911, 2025, 2111
TscAI CASTG 8 cut(s) 784, 817, 1023, 1260, 1624, 1990, 2077, 2322
TseFI GTSAC 4 cut(s) 1432, 2365, 2517, 2571
Tsp45I GTSAC 4 cut(s) 1432, 2365, 2517, 2571
TspDTI ATGAA 7 cut(s) 17, 463, 605, 1979, 2190, 2283, 2423
TspGWI ACGGA 4 cut(s) 211, 343, 373, 500
TspRI CASTG 8 cut(s) 784, 817, 1023, 1260, 1624, 1990, 2077, 2322
Tth111I GACNNNGTC 1 cut(s) 1430
Van91I CCANNNNNTGG 1 cut(s) 1713
Vha464I CTTAAG 2 cut(s) 458, 2024
VpaK11BI GGWCC 3 cut(s) 944, 1794, 2138
XagI CCTNNNNNAGG 1 cut(s) 1706
XapI RAATTY 4 cut(s) 735, 1356, 2732, 2746
XceI RCATGY 1 cut(s) 1906
XcmI CCANNNNNNNNNTGG 1 cut(s) 2413
XmiI GTMKAC 2 cut(s) 225, 2604
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 3 cut(s) 1556, 2285, 2466
ZraI GACGTC 2 cut(s) 217, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.