MD06G1128500.v1.1

Respiratory burst oxidase homolog protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004577
Physical Location & Seq
Forward (+)
27087364 .. 27094696
7333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1128500.v1.1.491

Sequence Viewer

Length: 2826 bp
ATGGGAACAAATGAGGCTGGAGGAGTTCATTACCACTCAGATACTGAGATCATCGGTGGTAACGAAAAAATGGCGAATTATAGCGGTCCGCTGAGCGGACCTCTTAGCGGACCACTGAACAAAAAAAGATCAGGCACAAAGAAGAATGCCAAGATCAACACACCGGAGCCAATCTCTGCTACCAAGCCTGGCATTAGTTCAAATGATGAAGAATCTTATGTCGAGGTCACTCTGGACGTCCGAGATGATTCTGTGGCGGTTCACAGCGTAAAACCTGCCGATCATCCTCCTCATGAAGATCACGAAGATCATGTTGAGCTGCTGGGAAAAGGGTTGGAGAAGAGCCGGTCTTTTGTTCGAAATGCTTCATTTCATATCAAGCGCCTGGCCTCGTTTTCAAAACAAGCAGTAGTAGCAGCAGTGCCTCATCATCATCCGCGAAGGCGGTTTGATCGAACACAGTCAGCGGCTGCCCCTGCATTGAAGGGCCTCAAGTTCATCAGCAAGACTGATGGGAATGAGTGGCCTGAAGTTGAGAAGAGGTTCTGCAAGCTCACTGCTTCCACCAATGGCCTGCTTTCCCGTGCGCTTTTCGGGGAATGTATAGGAATGAACAAGGAATCGAAAGAGTTTGCAGGGAAGCTTTTCGATGCGCTTGCTCGAAAGCTCGACATCAAAGGCGATTCTATCAATGAGGATCAGCTGAAAGAGTTATGGGATCAAATCTCTGAGCAGGGATTTGACTCCAGGCTTCAAACTTTCTTTGACATGGTTGATAAAGATGCAGATGGAAGAATCACCATAGAGGAAGTCAAGGAGATTATTAGCTTCAGTGCTTCTGCCAACAAGCTCTCAAACATCCAAAAACAAGCAGATGAGTATGCAGCATTGATCATGGAAGAGTTAGATCCAGACAATCTCGGATACATCATGATAGACAGCCTCGAAACGCTCTTGTTGGAAGGACCGGAAGAGACTGCAGCTGCGGCAAGAGGAAAAGACAGCCGAAACCTAAGCAGAATGCTGAGCCAGAAGCTCAAGCCTACAAGAGAGCATCCATTAACAAGGTGTTATAACAATACAAAGTACTTCTTGCAAGACAATTGGAAGAGAGTTTGGGTAATGATCCTGTGGCTCGGAATCACGTTAGGTCTATTCGTGTACAAATTCATGCAGTATCGAAACAAGGCAGCGTACGAAGTGATGGGACACTGCGTCTGCATGGCGAAAGGTGCAGCGGAGACACTCAAATTCAACATGGCGCTCATTCTCCTCCCGGTCTGCCGGAACACCATAACCTTGCTCCGAAACAAGACCAAACTAGGAGGCGTTATCCCCTTCGACGACAATCTCAATTTCCACAAGGTGATAGCTGTGGGGATTGGGTTTGGAGTTGGGATACATGCAATCTACCATATGGCCTGCGACTTCCCGAGGCTCATTCATGCGAACAGCGATGACTACGAGCTGATGAAGCCATTTTTCGGGGAGGTGCAACCTTCGAGTTACTGGTATTTCGTGAAATCTTTGGAGGGAGTGAGTGGGATTGTCATGGTGGTTTTGATGGCGGTTGCATTCACATTGGCTGCTCCTTGTTTCAGAAGAGGTAAAGTTAACCTGCCTAAGCCTCTGAATAAGCTCGCTGGGTTCAATGCCTTTTGGTATTCCCACCACCTTTTCGTCATAGTCTATGTCCTGCTGATTATCCATGGAGTTCACCTGTATCTGACCAAGAAATGGTACCAGAAAACAACATGGATGTACCTAGCTGTTCCACTCACACTCTATGCTGGTGAAAGATTAGTTAGGGCACTCAGATCCAGCATCAAGCCTGTTCAGATTCTAAAGGTGGCTCTCTACCCTGGTAATGTCTTGGCACTTCATTTGTCCAAGCCTCAAGGCTTCAGATACCATAGTGGGCAATACATGTTTGTTAATTGTGCTGCTGTGTCGCCATTTGAATGGCACCCGTTTTCCATCACCTCGGCCCCTGGAGATGACCACCTCAGTGTTCATATCCGGATCGTCGGAGATTGGACCCGACAACTTAAAACGGTGTTCTCAAAGGTATGCCAACCACCTCCTAGTGGGAAAAGTGGACTCCTTACAGCTGACCACATGCAAGGAGCTGAAAACTTGATATTTCCAAGAGTGTTGATCGATGGACCCTATGGAGCACCAGCTCAAGAATACAAGACCTATGAAGTGGTGTTGTTGGTAGGGCTAGGGATTGGGGCAACCCCAATGATTAGCATTTTGAAGGACATAGTGAACAATATCAAGGCCATGGAGGAAGAGGAGGAGGACAATGCTTTAGAAAATGGTGGTGGTGGTGGTGGCGGCACCAAGTTACCACACAAATCATCAAAAACACATGCTGGGTCGACCTCGGTGGGACACCATAACTTTAAGACCAAGCGGGCGTATTTTTATTGGGTGACACGGGAACAGGGCTCCTTTGATTGGTTCAAAGGAGCGATGAATGAGGTGGCGGAATTGGACCACAACAGGGTCATTGAATTTCACAATTATTGCACTAGTGTGTATGAAGAAGGTGATGCACGTTCTGCCCTCATTGCCATGCTTCAGTCTTTGAGTCATGCAAAGAATGGTGTCGACATTGTCTCCGGCACACGTGTCAAATCGCACTTTGCCAAGCCAAACTGGCGTGAGGTCTACAAGCGGATTGCACTCGATCACACCGATGCTAGAGTTGGGGTGTTCTACTGCGGGCCACCGGCGCTTACCGAAGAGCTACGCCAGCTGTCCCTCGACTTCTCTCACAAAACCTCCACCCGATTTGAGTTCCACAAAGAAAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

942

Amino Acids

105.38

Weight (kDa)

8.59

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 149 - 249 5.8e-35 Respiratory burst NADPH oxidase
EF-hand_7 PF13499 249 - 310 5.4e-06 EF-hand domain pair
Ferric_reduct PF01794 420 - 568 5e-11 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 611 - 728 3.4e-34 FAD-binding domain
NAD_binding_6 PF08030 734 - 923 1.8e-49 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1074
AatII GACGTC 1 cut(s) 240
Acc36I ACCTGC 2 cut(s) 283, 1626
Acc65I GGTACC 1 cut(s) 1740
AccB1I GGYRCC 3 cut(s) 1740, 1965, 2342
AccB7I CCANNNNNTGG 1 cut(s) 1737
AccBSI CCGCTC 1 cut(s) 96
AccI GTMKAC 3 cut(s) 2384, 2616, 2676
AccII CGCG 1 cut(s) 439
AccIII TCCGGA 1 cut(s) 2019
AclWI GGATC 6 cut(s) 705, 726, 902, 1120, 1812, 2030
AcsI RAATTY 3 cut(s) 1166, 1250, 2519
AcuI CTGAAG 4 cut(s) 549, 814, 1888, 2570
AcvI CACGTG 1 cut(s) 2636
AcyI GRCGYC 1 cut(s) 237
AdeI CACNNNGTG 1 cut(s) 1366
AfaI GTAC 5 cut(s) 1088, 1163, 1196, 1742, 1763
AfiI CCNNNNNNNGG 7 cut(s) 95, 107, 1484, 1737, 2087, 2463, 2509
AflIII ACRYGT 3 cut(s) 1926, 2633, 2635
AhdI GACNNNNNGTC 1 cut(s) 1214
AhlI ACTAGT 1 cut(s) 2537
AjnI CCWGG 5 cut(s) 187, 384, 746, 1861, 1990
AleI CACNNNNGTG 2 cut(s) 2007, 2540
Alw21I GWGCWC 1 cut(s) 2179
Alw26I GTCTC 3 cut(s) 968, 1235, 2629
AlwI GGATC 6 cut(s) 705, 726, 902, 1120, 1812, 2030
AlwNI CAGNNNCTG 2 cut(s) 44, 470
Ama87I CYCGRG 1 cut(s) 1432
Aor13HI TCCGGA 1 cut(s) 2019
AoxI GGCC 8 cut(s) 387, 487, 524, 571, 1419, 1986, 2283, 2732
ApoI RAATTY 3 cut(s) 1166, 1250, 2519
Asp700I GAANNNNTTC 3 cut(s) 364, 542, 644
Asp718I GGTACC 1 cut(s) 1740
AspLEI GCGC 5 cut(s) 384, 589, 655, 1264, 2743
AsuC2I CCSGG 1 cut(s) 1277
AsuHPI GGTGA 7 cut(s) 790, 1378, 1709, 1805, 1972, 2449, 2567
AsuII TTCGAA 1 cut(s) 358
AvaI CYCGRG 1 cut(s) 1432
AvaII GGWCC 7 cut(s) 86, 98, 110, 965, 2037, 2165, 2500
BaeGI GKGCMC 1 cut(s) 1813
BaeI ACNNNNGTAYC 2 cut(s) 1900, 1933
BanI GGYRCC 3 cut(s) 1740, 1965, 2342
BanII GRGCYC 1 cut(s) 2456
BbrPI CACGTG 1 cut(s) 2636
Bbv12I GWGCWC 1 cut(s) 2179
BccI CCATC 6 cut(s) 506, 782, 1198, 1558, 1985, 2156
BcgI CGANNNNNNTGC 2 cut(s) 638, 672
BciT130I CCWGG 5 cut(s) 189, 386, 748, 1863, 1992
BciVI GTATCC 2 cut(s) 917, 1392
BclI TGATCA 1 cut(s) 891
BcnI CCSGG 1 cut(s) 1277
BcoDI GTCTC 3 cut(s) 968, 1235, 2629
BcuI ACTAGT 1 cut(s) 2537
BfaI CTAG 6 cut(s) 1322, 1766, 2085, 2225, 2538, 2709
BfmI CTRYAG 1 cut(s) 978
BfoI RGCGCY 3 cut(s) 385, 1265, 2744
BfuAI ACCTGC 2 cut(s) 283, 1626
BfuI GTATCC 2 cut(s) 917, 1392
BglI GCCNNNNNGGC 1 cut(s) 2665
BlpI GCTNAGC 2 cut(s) 92, 1025
BmcAI AGTACT 1 cut(s) 1088
Bme1390I CCNGG 6 cut(s) 189, 386, 748, 1277, 1863, 1992
Bme18I GGWCC 7 cut(s) 86, 98, 110, 965, 2037, 2165, 2500
BmeRI GACNNNNNGTC 1 cut(s) 1214
BmeT110I CYCGRG 1 cut(s) 1432
BmiI GGNNCC 8 cut(s) 168, 1742, 1967, 1989, 2039, 2167, 2344, 2455
BmrFI CCNGG 6 cut(s) 189, 386, 748, 1277, 1863, 1992
BmsI GCATC 6 cut(s) 640, 772, 1063, 1833, 2548, 2695
BplI GAGNNNNNCTC 4 cut(s) 85, 117, 158, 190
BpmI CTGGAG 3 cut(s) 39, 730, 2013
Bpu10I CCTNAGC 2 cut(s) 1013, 1623
Bpu1102I GCTNAGC 2 cut(s) 92, 1025
Bpu14I TTCGAA 1 cut(s) 358
BpuEI CTTGAG 4 cut(s) 476, 1022, 1881, 2169
BpuMI CCSGG 1 cut(s) 1277
Bsa29I ATCGAT 1 cut(s) 2160
BsaAI YACGTR 1 cut(s) 2636
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 7 cut(s) 1433, 1708, 1861, 1983, 1990, 2286, 2388
BsaWI WCCGGW 3 cut(s) 163, 967, 2019
BsaXI ACNNNNNCTCC 4 cut(s) 2609, 2639, 2774, 2804
Bsc4I CCNNNNNNNGG 7 cut(s) 95, 107, 1484, 1737, 2087, 2463, 2509
Bse118I RCCGGY 2 cut(s) 345, 2737
Bse1I ACTGG 2 cut(s) 1514, 2669
Bse3DI GCAATG 1 cut(s) 2574
BseAI TCCGGA 1 cut(s) 2019
BseBI CCWGG 5 cut(s) 189, 386, 748, 1863, 1992
BseCI ATCGAT 1 cut(s) 2160
BseDI CCNNGG 7 cut(s) 1433, 1708, 1861, 1983, 1990, 2286, 2388
BseGI GGATG 5 cut(s) 283, 433, 858, 1054, 1764
BseLI CCNNNNNNNGG 7 cut(s) 95, 107, 1484, 1737, 2087, 2463, 2509
BseMI GCAATG 1 cut(s) 2574
BseMII CTCAG 7 cut(s) 36, 51, 83, 720, 1016, 1828, 2020
BseNI ACTGG 2 cut(s) 1514, 2669
BseRI GAGGAG 5 cut(s) 36, 279, 1262, 2312, 2315
BseSI GKGCMC 1 cut(s) 1813
BseYI CCCAGC 3 cut(s) 322, 1643, 2378
BsgI GTGCAG 1 cut(s) 1254
Bsh1236I CGCG 1 cut(s) 439
BshFI GGCC 8 cut(s) 389, 489, 526, 573, 1421, 1988, 2285, 2734
BshNI GGYRCC 3 cut(s) 1740, 1965, 2342
BshVI ATCGAT 1 cut(s) 2160
BsiHKAI GWGCWC 1 cut(s) 2179
BsiHKCI CYCGRG 1 cut(s) 1432
BsiSI CCGG 8 cut(s) 164, 346, 968, 1277, 1285, 2020, 2628, 2738
BsiWI CGTACG 1 cut(s) 1194
BslFI GGGAC 3 cut(s) 1221, 2409, 2752
BslI CCNNNNNNNGG 7 cut(s) 95, 107, 1484, 1737, 2087, 2463, 2509
BsmAI GTCTC 3 cut(s) 968, 1235, 2629
BsmFI GGGAC 3 cut(s) 1221, 2409, 2752
BsmI GAATGC 3 cut(s) 151, 1026, 1574
BsnI GGCC 8 cut(s) 389, 489, 526, 573, 1421, 1988, 2285, 2734
BsoBI CYCGRG 1 cut(s) 1432
Bsp119I TTCGAA 1 cut(s) 358
Bsp1286I GDGCHC 3 cut(s) 1813, 2179, 2456
Bsp13I TCCGGA 1 cut(s) 2019
Bsp1407I TGTACA 1 cut(s) 1161
Bsp1720I GCTNAGC 2 cut(s) 92, 1025
Bsp19I CCATGG 2 cut(s) 1708, 2286
BspANI GGCC 8 cut(s) 389, 489, 526, 573, 1421, 1988, 2285, 2734
BspCNI CTCAG 7 cut(s) 37, 50, 84, 721, 1017, 1827, 2019
BspDI ATCGAT 1 cut(s) 2160
BspEI TCCGGA 1 cut(s) 2019
BspFNI CGCG 1 cut(s) 439
BspHI TCATGA 2 cut(s) 292, 930
BspLI GGNNCC 8 cut(s) 168, 1742, 1967, 1989, 2039, 2167, 2344, 2455
BspMAI CTGCAG 1 cut(s) 982
BspMI ACCTGC 2 cut(s) 283, 1626
BspPI GGATC 6 cut(s) 705, 726, 902, 1120, 1812, 2030
BspQI GCTCTTC 2 cut(s) 335, 2745
BspT104I TTCGAA 1 cut(s) 358
BspT107I GGYRCC 3 cut(s) 1740, 1965, 2342
BsrBI CCGCTC 1 cut(s) 96
BsrDI GCAATG 1 cut(s) 2574
BsrFI RCCGGY 2 cut(s) 345, 2737
BsrGI TGTACA 1 cut(s) 1161
BsrI ACTGG 2 cut(s) 1514, 2669
BssAI RCCGGY 2 cut(s) 345, 2737
BssECI CCNNGG 7 cut(s) 1433, 1708, 1861, 1983, 1990, 2286, 2388
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 2 cut(s) 1708, 2286
Bst2UI CCWGG 5 cut(s) 189, 386, 748, 1863, 1992
Bst4CI ACNGT 2 cut(s) 462, 2056
Bst6I CTCTTC 8 cut(s) 335, 533, 894, 966, 1103, 1597, 2289, 2745
BstACI GRCGYC 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 2567
BstAUI TGTACA 1 cut(s) 1161
BstBAI YACGTR 1 cut(s) 2636
BstBI TTCGAA 1 cut(s) 358
BstC8I GCNNGC 8 cut(s) 551, 575, 657, 1423, 1641, 2421, 2732, 2762
BstDSI CCRYGG 2 cut(s) 1708, 2286
BstF5I GGATG 5 cut(s) 283, 433, 858, 1054, 1764
BstFNI CGCG 1 cut(s) 439
BstH2I RGCGCY 3 cut(s) 385, 1265, 2744
BstHHI GCGC 5 cut(s) 384, 589, 655, 1264, 2743
BstMAI GTCTC 3 cut(s) 968, 1235, 2629
BstNI CCWGG 5 cut(s) 189, 386, 748, 1863, 1992
BstNSI RCATGY 4 cut(s) 1406, 1930, 2122, 2378
BstSCI CCNGG 6 cut(s) 187, 384, 746, 1275, 1861, 1990
BstSFI CTRYAG 1 cut(s) 978
BstSLI GKGCMC 1 cut(s) 1813
BstUI CGCG 1 cut(s) 439
BstX2I RGATCY 2 cut(s) 907, 1817
BstXI CCANNNNNNTGG 1 cut(s) 1962
BstYI RGATCY 2 cut(s) 907, 1817
Bsu15I ATCGAT 1 cut(s) 2160
BsuI GTATCC 2 cut(s) 917, 1392
BsuRI GGCC 8 cut(s) 389, 489, 526, 573, 1421, 1988, 2285, 2734
BsuTUI ATCGAT 1 cut(s) 2160
BtgI CCRYGG 2 cut(s) 1708, 2286
BtgZI GCGATG 2 cut(s) 1470, 2492
BtsCI GGATG 5 cut(s) 283, 433, 858, 1054, 1764
BtsI GCAGTG 3 cut(s) 426, 555, 1210
BtsIMutI CAGTG 6 cut(s) 113, 426, 555, 838, 1210, 2014
BveI ACCTGC 2 cut(s) 283, 1626
Cac8I GCNNGC 8 cut(s) 551, 575, 657, 1423, 1641, 2421, 2732, 2762
CaiI CAGNNNCTG 2 cut(s) 44, 470
CciI TCATGA 2 cut(s) 292, 930
CfoI GCGC 5 cut(s) 384, 589, 655, 1264, 2743
Cfr10I RCCGGY 2 cut(s) 345, 2737
ClaI ATCGAT 1 cut(s) 2160
CpoI CGGWCCG 1 cut(s) 86
CseI GACGC 1 cut(s) 1204
Csp6I GTAC 5 cut(s) 1087, 1162, 1195, 1741, 1762
CspCI CAANNNNNGTGG 2 cut(s) 2782, 2817
CspI CGGWCCG 1 cut(s) 86
CviQI GTAC 5 cut(s) 1087, 1162, 1195, 1741, 1762
DraIII CACNNNGTG 1 cut(s) 1366
DriI GACNNNNNGTC 1 cut(s) 1214
Eam1104I CTCTTC 8 cut(s) 335, 533, 894, 966, 1103, 1597, 2289, 2745
Eam1105I GACNNNNNGTC 1 cut(s) 1214
EarI CTCTTC 8 cut(s) 335, 533, 894, 966, 1103, 1597, 2289, 2745
EciI GGCGGA 1 cut(s) 2507
Eco130I CCWWGG 2 cut(s) 1708, 2286
Eco24I GRGCYC 1 cut(s) 2456
Eco47I GGWCC 7 cut(s) 86, 98, 110, 965, 2037, 2165, 2500
Eco57I CTGAAG 4 cut(s) 549, 814, 1888, 2570
Eco72I CACGTG 1 cut(s) 2636
Eco88I CYCGRG 1 cut(s) 1432
EcoO109I RGGNCCY 1 cut(s) 487
EcoRII CCWGG 5 cut(s) 187, 384, 746, 1861, 1990
EcoT14I CCWWGG 2 cut(s) 1708, 2286
EcoT38I GRGCYC 1 cut(s) 2456
ErhI CCWWGG 2 cut(s) 1708, 2286
FalI AAGNNNNNCTT 2 cut(s) 1076, 1108
FaqI GGGAC 3 cut(s) 1221, 2409, 2752
FauI CCCGC 2 cut(s) 2412, 2723
FauNDI CATATG 1 cut(s) 1416
FbaI TGATCA 1 cut(s) 891
FblI GTMKAC 3 cut(s) 2384, 2616, 2676
FokI GGATG 5 cut(s) 270, 420, 845, 1041, 1771
FriOI GRGCYC 1 cut(s) 2456
FspBI CTAG 6 cut(s) 1322, 1766, 2085, 2225, 2538, 2709
GlaI GCGC 5 cut(s) 383, 588, 654, 1263, 2742
GsaI CCCAGC 3 cut(s) 326, 1647, 2382
GsuI CTGGAG 3 cut(s) 39, 730, 2013
HaeII RGCGCY 3 cut(s) 385, 1265, 2744
HaeIII GGCC 8 cut(s) 389, 489, 526, 573, 1421, 1988, 2285, 2734
HapII CCGG 8 cut(s) 164, 346, 968, 1277, 1285, 2020, 2628, 2738
HgaI GACGC 1 cut(s) 1204
HhaI GCGC 5 cut(s) 384, 589, 655, 1264, 2743
Hin1I GRCGYC 1 cut(s) 237
Hin6I GCGC 5 cut(s) 382, 587, 653, 1262, 2741
HinP1I GCGC 5 cut(s) 382, 587, 653, 1262, 2741
HincII GTYRAC 3 cut(s) 1615, 2385, 2617
HindII GTYRAC 3 cut(s) 1615, 2385, 2617
HindIII AAGCTT 1 cut(s) 641
HpaI GTTAAC 1 cut(s) 1615
HpaII CCGG 8 cut(s) 164, 346, 968, 1277, 1285, 2020, 2628, 2738
HphI GGTGA 7 cut(s) 790, 1378, 1709, 1805, 1972, 2449, 2567
Hpy166II GTNNAC 9 cut(s) 262, 1162, 1615, 1717, 2099, 2272, 2385, 2617, 2677
Hpy188III TCNNGA 9 cut(s) 233, 293, 302, 911, 931, 1432, 1519, 2020, 2186
Hpy8I GTNNAC 9 cut(s) 262, 1162, 1615, 1717, 2099, 2272, 2385, 2617, 2677
Hpy99I CGWCG 2 cut(s) 1346, 2030
HpyAV CCTTC 7 cut(s) 435, 478, 956, 1348, 1509, 2254, 2546
HpyCH4III ACNGT 2 cut(s) 462, 2056
HpyCH4IV ACGT 4 cut(s) 237, 1145, 2563, 2635
HpySE526I ACGT 4 cut(s) 237, 1145, 2563, 2635
Hsp92I GRCGYC 1 cut(s) 237
HspAI GCGC 5 cut(s) 382, 587, 653, 1262, 2741
Kpn2I TCCGGA 1 cut(s) 2019
KpnI GGTACC 1 cut(s) 1744
Ksp22I TGATCA 1 cut(s) 891
KspAI GTTAAC 1 cut(s) 1615
LguI GCTCTTC 2 cut(s) 335, 2745
LmnI GCTCC 7 cut(s) 166, 1308, 1594, 2126, 2174, 2459, 2474
LweI GCATC 6 cut(s) 640, 772, 1063, 1833, 2548, 2695
MaeI CTAG 6 cut(s) 1322, 1766, 2085, 2225, 2538, 2709
MaeII ACGT 4 cut(s) 237, 1145, 2563, 2635
MaeIII GTNAC 5 cut(s) 59, 226, 1505, 2349, 2437
MbiI CCGCTC 1 cut(s) 96
MfeI CAATTG 1 cut(s) 1102
MflI RGATCY 2 cut(s) 907, 1817
MhlI GDGCHC 3 cut(s) 1813, 2179, 2456
MluCI AATT 9 cut(s) 76, 1102, 1166, 1250, 1354, 1936, 2495, 2519, 2527
MlyI GAGTC 3 cut(s) 737, 2094, 2605
MmeI TCCRAC 3 cut(s) 315, 939, 2008
MroI TCCGGA 1 cut(s) 2019
MroXI GAANNNNTTC 3 cut(s) 364, 542, 644
MseI TTAA 5 cut(s) 1061, 1614, 1935, 2049, 2409
MslI CAYNNNNRTG 5 cut(s) 1960, 2007, 2540, 2579, 2703
MspA1I CMGCKG 7 cut(s) 91, 467, 703, 983, 1238, 2111, 2764
MspI CCGG 8 cut(s) 164, 346, 968, 1277, 1285, 2020, 2628, 2738
MspR9I CCNGG 6 cut(s) 189, 386, 748, 1277, 1863, 1992
MunI CAATTG 1 cut(s) 1102
Mva1269I GAATGC 3 cut(s) 151, 1026, 1574
MvaI CCWGG 5 cut(s) 189, 386, 748, 1863, 1992
MvnI CGCG 1 cut(s) 439
NciI CCSGG 1 cut(s) 1277
NcoI CCATGG 2 cut(s) 1708, 2286
NdeI CATATG 1 cut(s) 1416
NlaIV GGNNCC 8 cut(s) 168, 1742, 1967, 1989, 2039, 2167, 2344, 2455
NmeAIII GCCGAG 1 cut(s) 1964
NmuCI GTSAC 2 cut(s) 226, 2437
NspI RCATGY 4 cut(s) 1406, 1930, 2122, 2378
NspV TTCGAA 1 cut(s) 358
OliI CACNNNNGTG 2 cut(s) 2007, 2540
PagI TCATGA 2 cut(s) 292, 930
PciI ACATGT 1 cut(s) 1926
PciSI GCTCTTC 2 cut(s) 335, 2745
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 2700
PctI GAATGC 3 cut(s) 151, 1026, 1574
PdmI GAANNNNTTC 3 cut(s) 364, 542, 644
PfeI GAWTC 7 cut(s) 212, 248, 620, 683, 795, 1140, 1840
Pfl23II CGTACG 1 cut(s) 1194
PflFI GACNNNGTC 1 cut(s) 2621
PflMI CCANNNNNTGG 1 cut(s) 1737
PleI GAGTC 3 cut(s) 737, 2094, 2604
PmaCI CACGTG 1 cut(s) 2636
PmlI CACGTG 1 cut(s) 2636
PpsI GAGTC 3 cut(s) 737, 2094, 2604
Ppu21I YACGTR 1 cut(s) 2636
PscI ACATGT 1 cut(s) 1926
PsiI TTATAA 1 cut(s) 1074
Psp6I CCWGG 5 cut(s) 187, 384, 746, 1861, 1990
PspCI CACGTG 1 cut(s) 2636
PspFI CCCAGC 3 cut(s) 322, 1643, 2378
PspGI CCWGG 5 cut(s) 187, 384, 746, 1861, 1990
PspLI CGTACG 1 cut(s) 1194
PspN4I GGNNCC 8 cut(s) 168, 1742, 1967, 1989, 2039, 2167, 2344, 2455
PstI CTGCAG 1 cut(s) 982
PstNI CAGNNNCTG 2 cut(s) 44, 470
PsuI RGATCY 2 cut(s) 907, 1817
PsyI GACNNNGTC 1 cut(s) 2621
PvuII CAGCTG 4 cut(s) 703, 983, 2111, 2764
RsaI GTAC 5 cut(s) 1088, 1163, 1196, 1742, 1763
RsaNI GTAC 5 cut(s) 1087, 1162, 1195, 1741, 1762
RseI CAYNNNNRTG 5 cut(s) 1960, 2007, 2540, 2579, 2703
Rsr2I CGGWCCG 1 cut(s) 86
RsrII CGGWCCG 1 cut(s) 86
SalI GTCGAC 2 cut(s) 2383, 2615
SapI GCTCTTC 2 cut(s) 335, 2745
SaqAI TTAA 5 cut(s) 1061, 1614, 1935, 2049, 2409
ScaI AGTACT 1 cut(s) 1088
SchI GAGTC 3 cut(s) 737, 2094, 2605
ScrFI CCNGG 6 cut(s) 189, 386, 748, 1277, 1863, 1992
SduI GDGCHC 3 cut(s) 1813, 2179, 2456
SfaNI GCATC 6 cut(s) 640, 772, 1063, 1833, 2548, 2695
SfcI CTRYAG 1 cut(s) 978
SfuI TTCGAA 1 cut(s) 358
SgrAI CRCCGGYG 1 cut(s) 2737
SinI GGWCC 7 cut(s) 86, 98, 110, 965, 2037, 2165, 2500
SmiMI CAYNNNNRTG 5 cut(s) 1960, 2007, 2540, 2579, 2703
SmlI CTYRAG 4 cut(s) 491, 1037, 1896, 2184
SmoI CTYRAG 4 cut(s) 491, 1037, 1896, 2184
SpeI ACTAGT 1 cut(s) 2537
Sse9I AATT 9 cut(s) 76, 1102, 1166, 1250, 1354, 1936, 2495, 2519, 2527
SspMI CTAG 6 cut(s) 1322, 1766, 2085, 2225, 2538, 2709
StyD4I CCNGG 6 cut(s) 187, 384, 746, 1275, 1861, 1990
StyI CCWWGG 2 cut(s) 1708, 2286
TaaI ACNGT 2 cut(s) 462, 2056
TaiI ACGT 4 cut(s) 240, 1148, 2566, 2638
TasI AATT 9 cut(s) 76, 1102, 1166, 1250, 1354, 1936, 2495, 2519, 2527
TatI WGTACW 2 cut(s) 1086, 1161
TauI GCSGC 3 cut(s) 470, 989, 2343
TfiI GAWTC 7 cut(s) 212, 248, 620, 683, 795, 1140, 1840
Tru1I TTAA 5 cut(s) 1061, 1614, 1935, 2049, 2409
Tru9I TTAA 5 cut(s) 1061, 1614, 1935, 2049, 2409
TscAI CASTG 6 cut(s) 120, 426, 562, 838, 1217, 2014
TseFI GTSAC 2 cut(s) 226, 2437
Tsp45I GTSAC 2 cut(s) 226, 2437
TspRI CASTG 6 cut(s) 120, 426, 562, 838, 1217, 2014
Tth111I GACNNNGTC 1 cut(s) 2621
Van91I CCANNNNNTGG 1 cut(s) 1737
VpaK11BI GGWCC 7 cut(s) 86, 98, 110, 965, 2037, 2165, 2500
XapI RAATTY 3 cut(s) 1166, 1250, 2519
XceI RCATGY 4 cut(s) 1406, 1930, 2122, 2378
XmiI GTMKAC 3 cut(s) 2384, 2616, 2676
XmnI GAANNNNTTC 3 cut(s) 364, 542, 644
XspI CTAG 6 cut(s) 1322, 1766, 2085, 2225, 2538, 2709
ZraI GACGTC 1 cut(s) 238
ZrmI AGTACT 1 cut(s) 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.