FvH4_5g10600

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013309
Physical Location & Seq
Forward (+)
6021151 .. 6025569
4419 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10600.t2

Sequence Viewer

Length: 1263 bp
ATGGAGCCCTTTAGCCACTGTTCTTCATTGTTCTTCCTCGTTCTGCTAATTTCAGGGTTCCTCTACTCTGAAGTGGCGAACTCAATTGGAATCAACTACGGCCAGATTGCCAACAACTTACCTTCACCGGAAGCCGTGGTGCCCATAGCGAAATCCATCGGAATCACCAGAATCAAGCTCTACGACGCCGATCCCAAAGTCCTCAAGGCATTCGCCAACACCGGAGTGGAGTTCATAGTGGGCCTCGGGAACGAGTACCTCGCCAAGGTCCAAGATCCGGGGAAAGCCCAAGAATGGGTCAAGTCCAACGTCCAGGCTTACCTCCCGGCCACCAAGATCACCTGCATCTTCGTCGGCAACGAAGTCCTCACATTCAACGACACGTCGTTAACCGGCAATCTCCTCCCGGCGATGCAGAGCGTCCACACCGCGCTGGTCAATCTAAACCTGGACAAGCAGGTCACCGTCACCACTGCTCATTCCCTCTCCATTCTTGAAAATTCTTTCCCGCCGTCGGCCGGAACTTTCCGCCGGGATATAAAGAACTGCATAGCTCCGATCCTCGATTTCCATTCCAAAACCGGATCCCCGTTCTTAATCAACGCCTACCCTTACTTCGCATACAAATCAAACCCGAAGCAAGTTCCATTGGACTACGTCCTCTTCCAGCCCAACGCCGGATCGCCGGATCCGGCGACTAATCTCCACTACGACAACATGCTCTTCGCTCAGATCGACGCAGTCTACGCCGCGCTGGGATCTCTGGGCTACAAAAAACTCCCAGTTCACATCTCCGAGACAGGGTGGCCTTCTAAAGGCGACGACGACGAGGCCGGAGCCACGCCGGAGAATGCGAAGAAGTACAACGGGAATCTGATCAAGCTGATTTGCCAGAATAAAGGCACGCCGTTGAGGCCGAACTCGGATCTGAATATCTACGTGTTTGCTCTGTTTAATGAGAACATGAAACCCGGCCCGAGCTCCGAGAGGAACTTCGGGCTTTTGAAACCTGATGGAACTCCGGCGTACTCGCTTGGATTCTCGCTGAACAGTAGCTCAAGTAGTAAGGGCAACAGTAGCAGTAGCAGCTCGTCCGGGTCGGGTCAGGGTGGGATTAATCCGATTACTATCGGATCGTCGCCGCCTGGTTCTTCCAGTAACGGCTACTTGTCCATCTCATCCGCCTCCGCCACTAAGAGATACCATATGATCGGCTCGATTTCAATGTTGTGGATGGTCCTATTGTTGATAAAAGTCATTTGA

Protein Analysis

421

Amino Acids

45.05

Weight (kDa)

8.16

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 29 - 347 6.3e-86 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 350
AasI GACNNNNNNGTC 1 cut(s) 458
Acc36I ACCTGC 2 cut(s) 350, 448
AccB1I GGYRCC 1 cut(s) 139
AccI GTMKAC 1 cut(s) 744
AccII CGCG 2 cut(s) 431, 752
AciI CCGC 7 cut(s) 429, 509, 529, 750, 1142, 1182, 1188
AcoI YGGCCR 3 cut(s) 100, 327, 516
AcsI RAATTY 1 cut(s) 499
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 3 cut(s) 257, 863, 1028
AfiI CCNNNNNNNGG 9 cut(s) 265, 277, 294, 295, 514, 518, 677, 801, 815
AflIII ACRYGT 2 cut(s) 381, 939
AgsI TTSAA 4 cut(s) 376, 497, 1006, 1224
AjiI CACGTC 1 cut(s) 384
AjnI CCWGG 3 cut(s) 312, 447, 1144
AleI CACNNNNGTG 1 cut(s) 224
AluBI AGCT 6 cut(s) 178, 554, 883, 981, 1056, 1089
AluI AGCT 6 cut(s) 178, 554, 883, 981, 1056, 1089
Alw21I GWGCWC 1 cut(s) 983
Alw26I GTCTC 1 cut(s) 791
Ama87I CYCGRG 2 cut(s) 245, 976
AoxI GGCC 8 cut(s) 100, 241, 327, 516, 806, 831, 914, 973
ApeKI GCWGC 1 cut(s) 1086
ApoI RAATTY 1 cut(s) 499
AseI ATTAAT 1 cut(s) 1116
AspLEI GCGC 2 cut(s) 433, 754
AspS9I GGNCC 4 cut(s) 241, 268, 974, 1237
AsuC2I CCSGG 6 cut(s) 279, 326, 407, 533, 972, 1096
AsuHPI GGTGA 5 cut(s) 117, 157, 331, 454, 460
AvaI CYCGRG 2 cut(s) 245, 976
AvaII GGWCC 2 cut(s) 268, 1237
BaeGI GKGCMC 1 cut(s) 144
BamHI GGATCC 2 cut(s) 584, 688
BanI GGYRCC 1 cut(s) 139
BanII GRGCYC 2 cut(s) 9, 983
Bbv12I GWGCWC 1 cut(s) 983
BbvI GCAGC 1 cut(s) 1098
BccI CCATC 4 cut(s) 164, 1007, 1181, 1228
BceAI ACGGC 5 cut(s) 115, 119, 496, 892, 1177
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BciT130I CCWGG 3 cut(s) 314, 449, 1146
BclI TGATCA 1 cut(s) 876
BcnI CCSGG 6 cut(s) 279, 326, 407, 533, 972, 1096
BcoDI GTCTC 1 cut(s) 791
BfuAI ACCTGC 2 cut(s) 350, 448
BglI GCCNNNNNGGC 1 cut(s) 913
BisI GCNGC 3 cut(s) 750, 1087, 1142
BlsI GCNGC 3 cut(s) 751, 1088, 1143
Bme1390I CCNGG 9 cut(s) 279, 314, 326, 407, 449, 533, 972, 1096, 1146
Bme18I GGWCC 2 cut(s) 268, 1237
BmeT110I CYCGRG 2 cut(s) 245, 976
BmgBI CACGTC 1 cut(s) 384
BmgT120I GGNCC 4 cut(s) 241, 268, 974, 1237
BmiI GGNNCC 6 cut(s) 6, 59, 141, 586, 690, 838
BmrFI CCNGG 9 cut(s) 279, 314, 326, 407, 449, 533, 972, 1096, 1146
BmrI ACTGGG 1 cut(s) 776
BmsI GCATC 2 cut(s) 354, 402
BmuI ACTGGG 1 cut(s) 776
BpuEI CTTGAG 2 cut(s) 188, 1042
BpuMI CCSGG 6 cut(s) 279, 326, 407, 533, 972, 1096
BsaAI YACGTR 1 cut(s) 940
BsaBI GATNNNNATC 1 cut(s) 1127
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 4 cut(s) 135, 244, 264, 278
BsaWI WCCGGW 3 cut(s) 127, 221, 581
Bsc4I CCNNNNNNNGG 9 cut(s) 265, 277, 294, 295, 514, 518, 677, 801, 815
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 2 cut(s) 782, 1155
Bse8I GATNNNNATC 1 cut(s) 1127
BseBI CCWGG 3 cut(s) 314, 449, 1146
BseDI CCNNGG 4 cut(s) 135, 244, 264, 278
BseGI GGATG 2 cut(s) 1178, 1239
BseJI GATNNNNATC 1 cut(s) 1127
BseLI CCNNNNNNNGG 9 cut(s) 265, 277, 294, 295, 514, 518, 677, 801, 815
BseMII CTCAG 1 cut(s) 743
BseNI ACTGG 2 cut(s) 782, 1155
BseRI GAGGAG 1 cut(s) 392
BseSI GKGCMC 1 cut(s) 144
BseX3I CGGCCG 1 cut(s) 516
BseXI GCAGC 1 cut(s) 1098
BseYI CCCAGC 1 cut(s) 754
Bsh1236I CGCG 2 cut(s) 431, 752
Bsh1285I CGRYCG 1 cut(s) 519
BshFI GGCC 8 cut(s) 102, 243, 329, 518, 808, 833, 916, 975
BshNI GGYRCC 1 cut(s) 139
BsiEI CGRYCG 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 983
BsiHKCI CYCGRG 2 cut(s) 245, 976
BslI CCNNNNNNNGG 9 cut(s) 265, 277, 294, 295, 514, 518, 677, 801, 815
BsmAI GTCTC 1 cut(s) 791
BsmI GAATGC 2 cut(s) 209, 856
BsnI GGCC 8 cut(s) 102, 243, 329, 518, 808, 833, 916, 975
BsoBI CYCGRG 2 cut(s) 245, 976
Bsp1286I GDGCHC 3 cut(s) 9, 144, 983
BspACI CCGC 7 cut(s) 429, 509, 529, 750, 1142, 1182, 1188
BspANI GGCC 8 cut(s) 102, 243, 329, 518, 808, 833, 916, 975
BspCNI CTCAG 1 cut(s) 742
BspFNI CGCG 2 cut(s) 431, 752
BspLI GGNNCC 6 cut(s) 6, 59, 141, 586, 690, 838
BspMI ACCTGC 2 cut(s) 350, 448
BspQI GCTCTTC 1 cut(s) 728
BspT107I GGYRCC 1 cut(s) 139
BsrFI RCCGGY 1 cut(s) 392
BsrI ACTGG 2 cut(s) 782, 1155
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 4 cut(s) 135, 244, 264, 278
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 3 cut(s) 314, 449, 1146
Bst4CI ACNGT 4 cut(s) 20, 466, 1052, 1076
Bst6I CTCTTC 2 cut(s) 668, 728
BstACI GRCGYC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 940
BstC8I GCNNGC 1 cut(s) 905
BstDEI CTNAG 2 cut(s) 729, 1194
BstDSI CCRYGG 1 cut(s) 135
BstEII GGTNACC 1 cut(s) 460
BstENI CCTNNNNNAGG 2 cut(s) 263, 813
BstF5I GGATG 2 cut(s) 1178, 1239
BstFNI CGCG 2 cut(s) 431, 752
BstHHI GCGC 2 cut(s) 433, 754
BstMAI GTCTC 1 cut(s) 791
BstMCI CGRYCG 1 cut(s) 519
BstMWI GCNNNNNNNGC 4 cut(s) 746, 913, 1077, 1086
BstNI CCWGG 3 cut(s) 314, 449, 1146
BstNSI RCATGY 1 cut(s) 721
BstPI GGTNACC 1 cut(s) 460
BstSCI CCNGG 9 cut(s) 277, 312, 324, 405, 447, 531, 970, 1094, 1144
BstSLI GKGCMC 1 cut(s) 144
BstUI CGCG 2 cut(s) 431, 752
BstV1I GCAGC 1 cut(s) 1098
BstX2I RGATCY 5 cut(s) 274, 584, 688, 758, 925
BstYI RGATCY 5 cut(s) 274, 584, 688, 758, 925
BstZI CGGCCG 1 cut(s) 516
BsuRI GGCC 8 cut(s) 102, 243, 329, 518, 808, 833, 916, 975
BtgI CCRYGG 1 cut(s) 135
BtgZI GCGATG 1 cut(s) 425
BtrI CACGTC 1 cut(s) 384
BtsCI GGATG 2 cut(s) 1178, 1239
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 2 cut(s) 16, 471
BveI ACCTGC 2 cut(s) 350, 448
Cac8I GCNNGC 1 cut(s) 905
CfoI GCGC 2 cut(s) 433, 754
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 4 cut(s) 241, 268, 974, 1237
CseI GACGC 3 cut(s) 194, 409, 746
Csp6I GTAC 3 cut(s) 256, 862, 1027
CviAII CATG 2 cut(s) 718, 964
CviQI GTAC 3 cut(s) 256, 862, 1027
DdeI CTNAG 2 cut(s) 729, 1194
DrdI GACNNNNNNGTC 1 cut(s) 458
DseDI GACNNNNNNGTC 1 cut(s) 458
EaeI YGGCCR 3 cut(s) 100, 327, 516
EagI CGGCCG 1 cut(s) 516
Eam1104I CTCTTC 2 cut(s) 668, 728
EarI CTCTTC 2 cut(s) 668, 728
EciI GGCGGA 3 cut(s) 518, 1171, 1177
Ecl136II GAGCTC 1 cut(s) 981
EclXI CGGCCG 1 cut(s) 516
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 2 cut(s) 9, 983
Eco47I GGWCC 2 cut(s) 268, 1237
Eco52I CGGCCG 1 cut(s) 516
Eco53kI GAGCTC 1 cut(s) 981
Eco57I CTGAAG 1 cut(s) 90
Eco88I CYCGRG 2 cut(s) 245, 976
Eco91I GGTNACC 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 981
EcoNI CCTNNNNNAGG 2 cut(s) 263, 813
EcoO65I GGTNACC 1 cut(s) 460
EcoRII CCWGG 3 cut(s) 312, 447, 1144
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 2 cut(s) 9, 983
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 2 cut(s) 721, 967
FaiI YATR 9 cut(s) 146, 236, 539, 551, 622, 719, 965, 1206, 1208
FatI CATG 2 cut(s) 717, 963
FauI CCCGC 1 cut(s) 516
FauNDI CATATG 1 cut(s) 1206
FbaI TGATCA 1 cut(s) 876
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 3 cut(s) 750, 1087, 1142
FokI GGATG 2 cut(s) 1165, 1246
FriOI GRGCYC 2 cut(s) 9, 983
Fsp4HI GCNGC 3 cut(s) 750, 1087, 1142
GlaI GCGC 2 cut(s) 432, 753
GluI GCNGC 3 cut(s) 750, 1087, 1142
GsaI CCCAGC 1 cut(s) 758
HaeIII GGCC 8 cut(s) 102, 243, 329, 518, 808, 833, 916, 975
HgaI GACGC 3 cut(s) 194, 409, 746
HhaI GCGC 2 cut(s) 433, 754
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 2 cut(s) 721, 967
Hin6I GCGC 2 cut(s) 431, 752
HinP1I GCGC 2 cut(s) 431, 752
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HinfI GANTC 5 cut(s) 90, 162, 171, 871, 1038
HpaI GTTAAC 1 cut(s) 390
HphI GGTGA 5 cut(s) 117, 157, 331, 454, 460
Hpy166II GTNNAC 4 cut(s) 390, 424, 745, 787
Hpy188III TCNNGA 2 cut(s) 247, 494
Hpy8I GTNNAC 4 cut(s) 390, 424, 745, 787
Hpy99I CGWCG 9 cut(s) 188, 356, 388, 517, 740, 824, 827, 830, 1141
HpyAV CCTTC 2 cut(s) 132, 819
HpyCH4III ACNGT 4 cut(s) 20, 466, 1052, 1076
HpyCH4IV ACGT 4 cut(s) 309, 383, 657, 939
HpyCH4V TGCA 3 cut(s) 345, 415, 549
HpyF10VI GCNNNNNNNGC 4 cut(s) 746, 913, 1077, 1086
HpyF3I CTNAG 2 cut(s) 729, 1194
HpySE526I ACGT 4 cut(s) 309, 383, 657, 939
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 2 cut(s) 721, 967
HspAI GCGC 2 cut(s) 431, 752
Ksp22I TGATCA 1 cut(s) 876
KspAI GTTAAC 1 cut(s) 390
LguI GCTCTTC 1 cut(s) 728
LmnI GCTCC 4 cut(s) 4, 559, 836, 986
Lsp1109I GCAGC 1 cut(s) 1098
LweI GCATC 2 cut(s) 354, 402
MaeII ACGT 4 cut(s) 309, 383, 657, 939
MaeIII GTNAC 3 cut(s) 460, 466, 1157
MboII GAAGA 7 cut(s) 15, 25, 340, 655, 715, 868, 1143
MfeI CAATTG 1 cut(s) 84
MflI RGATCY 5 cut(s) 274, 584, 688, 758, 925
MhlI GDGCHC 3 cut(s) 9, 144, 983
MluCI AATT 3 cut(s) 48, 84, 499
MmeI TCCRAC 1 cut(s) 330
MseI TTAA 4 cut(s) 389, 596, 954, 1116
MslI CAYNNNNRTG 1 cut(s) 224
MspR9I CCNGG 9 cut(s) 279, 314, 326, 407, 449, 533, 972, 1096, 1146
MunI CAATTG 1 cut(s) 84
Mva1269I GAATGC 2 cut(s) 209, 856
MvaI CCWGG 3 cut(s) 314, 449, 1146
MvnI CGCG 2 cut(s) 431, 752
MwoI GCNNNNNNNGC 4 cut(s) 746, 913, 1077, 1086
NciI CCSGG 6 cut(s) 279, 326, 407, 533, 972, 1096
NdeI CATATG 1 cut(s) 1206
NlaIII CATG 2 cut(s) 721, 967
NlaIV GGNNCC 6 cut(s) 6, 59, 141, 586, 690, 838
NmuCI GTSAC 2 cut(s) 460, 466
NspI RCATGY 1 cut(s) 721
OliI CACNNNNGTG 1 cut(s) 224
PaqCI CACCTGC 1 cut(s) 350
PciSI GCTCTTC 1 cut(s) 728
PcsI WCGNNNNNNNCGW 2 cut(s) 357, 732
PctI GAATGC 2 cut(s) 209, 856
PfeI GAWTC 5 cut(s) 90, 162, 171, 871, 1038
PflFI GACNNNGTC 2 cut(s) 656, 740
PkrI GCNGC 3 cut(s) 751, 1088, 1143
Ppu21I YACGTR 1 cut(s) 940
PshBI ATTAAT 1 cut(s) 1116
Psp124BI GAGCTC 1 cut(s) 983
Psp6I CCWGG 3 cut(s) 312, 447, 1144
PspEI GGTNACC 1 cut(s) 460
PspFI CCCAGC 1 cut(s) 754
PspGI CCWGG 3 cut(s) 312, 447, 1144
PspN4I GGNNCC 6 cut(s) 6, 59, 141, 586, 690, 838
PspPI GGNCC 4 cut(s) 241, 268, 974, 1237
PsuI RGATCY 5 cut(s) 274, 584, 688, 758, 925
PsyI GACNNNGTC 2 cut(s) 656, 740
RsaI GTAC 3 cut(s) 257, 863, 1028
RsaNI GTAC 3 cut(s) 256, 862, 1027
RseI CAYNNNNRTG 1 cut(s) 224
SacI GAGCTC 1 cut(s) 983
SapI GCTCTTC 1 cut(s) 728
SaqAI TTAA 4 cut(s) 389, 596, 954, 1116
SatI GCNGC 3 cut(s) 750, 1087, 1142
Sau96I GGNCC 4 cut(s) 241, 268, 974, 1237
ScrFI CCNGG 9 cut(s) 279, 314, 326, 407, 449, 533, 972, 1096, 1146
SduI GDGCHC 3 cut(s) 9, 144, 983
SfaNI GCATC 2 cut(s) 354, 402
SinI GGWCC 2 cut(s) 268, 1237
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 2 cut(s) 203, 1057
SmoI CTYRAG 2 cut(s) 203, 1057
Sse9I AATT 3 cut(s) 48, 84, 499
SsiI CCGC 7 cut(s) 429, 509, 529, 750, 1142, 1182, 1188
SstI GAGCTC 1 cut(s) 983
StyD4I CCNGG 9 cut(s) 277, 312, 324, 405, 447, 531, 970, 1094, 1144
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 4 cut(s) 20, 466, 1052, 1076
TaiI ACGT 4 cut(s) 312, 386, 660, 942
TaqI TCGA 3 cut(s) 564, 735, 1217
TasI AATT 3 cut(s) 48, 84, 499
TatI WGTACW 1 cut(s) 861
TauI GCSGC 2 cut(s) 752, 1144
TfiI GAWTC 5 cut(s) 90, 162, 171, 871, 1038
Tru1I TTAA 4 cut(s) 389, 596, 954, 1116
Tru9I TTAA 4 cut(s) 389, 596, 954, 1116
TscAI CASTG 2 cut(s) 23, 478
TseFI GTSAC 2 cut(s) 460, 466
TseI GCWGC 1 cut(s) 1086
Tsp45I GTSAC 2 cut(s) 460, 466
TspDTI ATGAA 3 cut(s) 15, 223, 980
TspRI CASTG 2 cut(s) 23, 478
Tth111I GACNNNGTC 2 cut(s) 656, 740
VpaK11BI GGWCC 2 cut(s) 268, 1237
VspI ATTAAT 1 cut(s) 1116
XagI CCTNNNNNAGG 2 cut(s) 263, 813
XapI RAATTY 1 cut(s) 499
XceI RCATGY 1 cut(s) 721
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.