MD14G1138400.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013309
Physical Location & Seq
Reverse (-)
22657077 .. 22660545
3469 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1138400.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGCGGCCACCACCAGCTTCCCCTACCCCTCTGCAATTTTCCTGTCAGTTCTCATCATTTCAGGGTTCATCTTTCCAGAAACGGTAAATTCAATGGGAATAAACTACGGCCAAATCGCCAACAATTTACCGTTACCGGACGACGTCGTTCCGCTGGTGAAGTCCATCGGCATCTCCAAGATCAAGCTTTACGACGCCGATCCCAAGGTCCTCAAAGCCTTCGCCAACACCGGCGTCGAGTTCATAGTGGGCCTCCCCAACGGCTACCTCTCCAAGGTCCAGGACCCGGCCCAGGCCCAATCCTGGGTAAAGTCCAACGTCCAGGCCCACCTCCCCGCCACCAACATCACAAGCATCTTCGTCGGCAACGAGGTCCTCACCCTCAACGACACGTCGTTGAACAACAACCTCCTTCCGGCAATGCAGAGCGTCCACACCGCCCTCGTTGGTCTAGGCCTCGACAAGCAAGTCGCCGTCACCACCGCCCACTCCCTCTCCATTCTGGAGACCTCGTTTCCGCCCTCCGCCGGCGCTTTCCGCCGAGATCTGACTAGCTGCATCACTCCGATCCTCAATTTCCACGCCAAAACCGGATCGCCGTTCCTGATCAACGCCTACCCTTACTTCGCCTACAAGTCGAACCCTAAGCAGGTGCAGCTCGACTATGTCCTCTTCCAGCCGAACTCCGGCTCCGTTGATCCCGCCACCAATCTCCGCTACGACAACATGCTCTTCGCCCAGATCGACGCCGTGTACTCCGCCCTGGGCTCCCTCGGTTTCAAGAAGCTCCCGGTCCACATCTCCGAGACCGGGTGGCCGTCGAAGGGAGATGAGGACGAGGCCGGAGCGACGCCGGAGAACGCGAAGAAGTACAACGGGAATCTGATCAAGCTCATTTGCCAGAAGAAGGGAACTCCGCTCAGGCCCGGGTCGGATCTCAACATCTACGTCTTCGCTCTATTCAACGAGAACATGAAACCCGGCCCGAGTTCAGAGAGGAACTTCGGGCTTTTTAAACCTGACGGGTCGCCCGCGTACCCAATCGAGCTCGCGGCGGGCAACATATCCCTTGGTAACTCCAGTTCCAGTTCCAGTTCGGGTTCGGGTTCGAGTTCGGGCCAGGGACAGGTGAATCCGATTAGTAACGGTGGGTCACCGTCAGATTCTTCCGGCACGCCATACTTGTCCATTTCCTCCGCCCCGGCCATGAGATGGGATGTAATTGGTGGGATTCCGATGACGTGGCTAGTGGTCTCGTTGGTGATGAAACACGTCCTTTGA

Protein Analysis

427

Amino Acids

45.28

Weight (kDa)

6.59

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 32 - 349 2.4e-84 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 640
AasI GACNNNNNNGTC 1 cut(s) 467
AatII GACGTC 1 cut(s) 147
Acc36I ACCTGC 1 cut(s) 640
AccB7I CCANNNNNTGG 1 cut(s) 1212
AccBSI CCGCTC 1 cut(s) 919
AccII CGCG 3 cut(s) 863, 1034, 1052
AclWI GGATC 5 cut(s) 194, 562, 601, 692, 942
AcoI YGGCCR 4 cut(s) 6, 109, 815, 1203
AcsI RAATTY 1 cut(s) 88
AcyI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
AfaI GTAC 3 cut(s) 755, 872, 1037
AflIII ACRYGT 2 cut(s) 390, 1270
AgsI TTSAA 4 cut(s) 93, 400, 781, 964
AjiI CACGTC 3 cut(s) 393, 1242, 1273
AjnI CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1119
AloI GAACNNNNNNTCC 2 cut(s) 674, 706
AluBI AGCT 7 cut(s) 18, 187, 555, 658, 787, 892, 1048
AluI AGCT 7 cut(s) 18, 187, 555, 658, 787, 892, 1048
Alw21I GWGCWC 1 cut(s) 1050
Alw26I GTCTC 3 cut(s) 500, 800, 1258
AlwI GGATC 5 cut(s) 194, 562, 601, 692, 942
Ama87I CYCGRG 2 cut(s) 926, 985
ApeKI GCWGC 2 cut(s) 555, 655
ApoI RAATTY 1 cut(s) 88
AspLEI GCGC 1 cut(s) 533
AsuC2I CCSGG 7 cut(s) 287, 791, 811, 927, 928, 981, 1202
AsuHPI GGTGA 6 cut(s) 169, 370, 469, 1141, 1146, 1273
AvaI CYCGRG 2 cut(s) 926, 985
AvaII GGWCC 5 cut(s) 208, 277, 283, 373, 793
BanII GRGCYC 2 cut(s) 770, 1050
BbsI GAAGAC 1 cut(s) 943
Bbv12I GWGCWC 1 cut(s) 1050
BbvI GCAGC 2 cut(s) 542, 667
BccI CCATC 2 cut(s) 173, 1206
BceAI ACGGC 6 cut(s) 124, 277, 458, 583, 734, 802
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BciT130I CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1121
BclI TGATCA 2 cut(s) 606, 885
BcnI CCSGG 7 cut(s) 287, 791, 811, 927, 928, 981, 1202
BcoDI GTCTC 3 cut(s) 500, 800, 1258
BfaI CTAG 3 cut(s) 452, 552, 1247
BfoI RGCGCY 1 cut(s) 534
BfuAI ACCTGC 1 cut(s) 640
BglII AGATCT 1 cut(s) 544
BisI GCNGC 4 cut(s) 6, 556, 656, 1053
BlsI GCNGC 4 cut(s) 7, 557, 657, 1054
Bme18I GGWCC 5 cut(s) 208, 277, 283, 373, 793
BmeT110I CYCGRG 2 cut(s) 926, 985
BmgBI CACGTC 3 cut(s) 393, 1242, 1273
BmiI GGNNCC 3 cut(s) 285, 691, 769
BmsI GCATC 3 cut(s) 180, 363, 567
BpiI GAAGAC 1 cut(s) 943
BpmI CTGGAG 2 cut(s) 524, 1063
Bpu10I CCTNAGC 2 cut(s) 645, 920
BpuMI CCSGG 7 cut(s) 287, 791, 811, 927, 928, 981, 1202
BsaHI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
BsaI GGTCTC 3 cut(s) 500, 800, 1258
BsaWI WCCGGW 2 cut(s) 136, 590
BsaXI ACNNNNNCTCC 6 cut(s) 479, 497, 509, 527, 674, 704
Bse118I RCCGGY 2 cut(s) 230, 527
Bse1I ACTGG 3 cut(s) 1080, 1086, 1092
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1121
BseGI GGATG 1 cut(s) 1222
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 934
BseNI ACTGG 3 cut(s) 1080, 1086, 1092
BseXI GCAGC 2 cut(s) 542, 667
BsgI GTGCAG 1 cut(s) 674
Bsh1236I CGCG 3 cut(s) 863, 1034, 1052
BsiHKAI GWGCWC 1 cut(s) 1050
BsiHKCI CYCGRG 2 cut(s) 926, 985
BslFI GGGAC 1 cut(s) 1137
BsmAI GTCTC 3 cut(s) 500, 800, 1258
BsmFI GGGAC 1 cut(s) 1137
Bso31I GGTCTC 3 cut(s) 500, 800, 1258
BsoBI CYCGRG 2 cut(s) 926, 985
Bsp1286I GDGCHC 2 cut(s) 770, 1050
BspCNI CTCAG 1 cut(s) 933
BspFNI CGCG 3 cut(s) 863, 1034, 1052
BspLI GGNNCC 3 cut(s) 285, 691, 769
BspMI ACCTGC 1 cut(s) 640
BspPI GGATC 5 cut(s) 194, 562, 601, 692, 942
BspQI GCTCTTC 1 cut(s) 737
BspTNI GGTCTC 3 cut(s) 500, 800, 1258
BsrBI CCGCTC 1 cut(s) 919
BsrDI GCAATG 1 cut(s) 426
BsrFI RCCGGY 2 cut(s) 230, 527
BsrI ACTGG 3 cut(s) 1080, 1086, 1092
BssAI RCCGGY 2 cut(s) 230, 527
BssNI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
BssT1I CCWWGG 3 cut(s) 204, 273, 1069
Bst2UI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1121
Bst4CI ACNGT 4 cut(s) 85, 132, 1148, 1158
Bst6I CTCTTC 2 cut(s) 677, 737
BstACI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
BstC8I GCNNGC 5 cut(s) 529, 1032, 1050, 1057, 1175
BstDEI CTNAG 2 cut(s) 645, 920
BstEII GGTNACC 1 cut(s) 1152
BstENI CCTNNNNNAGG 1 cut(s) 272
BstF5I GGATG 1 cut(s) 1222
BstFNI CGCG 3 cut(s) 863, 1034, 1052
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 1 cut(s) 533
BstMAI GTCTC 3 cut(s) 500, 800, 1258
BstMWI GCNNNNNNNGC 2 cut(s) 537, 655
BstNI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1121
BstNSI RCATGY 1 cut(s) 730
BstPI GGTNACC 1 cut(s) 1152
BstUI CGCG 3 cut(s) 863, 1034, 1052
BstV1I GCAGC 2 cut(s) 542, 667
BstV2I GAAGAC 1 cut(s) 943
BstX2I RGATCY 2 cut(s) 544, 934
BstYI RGATCY 2 cut(s) 544, 934
BtrI CACGTC 3 cut(s) 393, 1242, 1273
BtsCI GGATG 1 cut(s) 1222
BveI ACCTGC 1 cut(s) 640
Cac8I GCNNGC 5 cut(s) 529, 1032, 1050, 1057, 1175
CfoI GCGC 1 cut(s) 533
Cfr10I RCCGGY 2 cut(s) 230, 527
Cfr9I CCCGGG 1 cut(s) 926
CseI GACGC 5 cut(s) 203, 223, 418, 755, 859
Csp6I GTAC 3 cut(s) 754, 871, 1036
CviAII CATG 3 cut(s) 727, 973, 1207
CviQI GTAC 3 cut(s) 754, 871, 1036
DdeI CTNAG 2 cut(s) 645, 920
DraI TTTAAA 1 cut(s) 1015
DrdI GACNNNNNNGTC 1 cut(s) 467
DseDI GACNNNNNNGTC 1 cut(s) 467
EaeI YGGCCR 4 cut(s) 6, 109, 815, 1203
Eam1104I CTCTTC 2 cut(s) 677, 737
EarI CTCTTC 2 cut(s) 677, 737
EciI GGCGGA 5 cut(s) 507, 514, 527, 748, 1186
Ecl136II GAGCTC 1 cut(s) 1048
Eco130I CCWWGG 3 cut(s) 204, 273, 1069
Eco147I AGGCCT 1 cut(s) 456
Eco24I GRGCYC 2 cut(s) 770, 1050
Eco31I GGTCTC 3 cut(s) 500, 800, 1258
Eco47I GGWCC 5 cut(s) 208, 277, 283, 373, 793
Eco53kI GAGCTC 1 cut(s) 1048
Eco88I CYCGRG 2 cut(s) 926, 985
Eco91I GGTNACC 1 cut(s) 1152
EcoICRI GAGCTC 1 cut(s) 1048
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoO109I RGGNCCY 3 cut(s) 208, 283, 373
EcoO65I GGTNACC 1 cut(s) 1152
EcoRII CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1119
EcoT14I CCWWGG 3 cut(s) 204, 273, 1069
EcoT38I GRGCYC 2 cut(s) 770, 1050
ErhI CCWWGG 3 cut(s) 204, 273, 1069
FaeI CATG 3 cut(s) 730, 976, 1210
FaiI YATR 7 cut(s) 245, 666, 728, 974, 1064, 1180, 1208
FaqI GGGAC 1 cut(s) 1137
FatI CATG 3 cut(s) 726, 972, 1206
FauI CCCGC 4 cut(s) 343, 709, 1039, 1048
FbaI TGATCA 2 cut(s) 606, 885
Fnu4HI GCNGC 4 cut(s) 6, 556, 656, 1053
FokI GGATG 1 cut(s) 1229
FriOI GRGCYC 2 cut(s) 770, 1050
Fsp4HI GCNGC 4 cut(s) 6, 556, 656, 1053
FspBI CTAG 3 cut(s) 452, 552, 1247
GlaI GCGC 1 cut(s) 532
GluI GCNGC 4 cut(s) 6, 556, 656, 1053
GsuI CTGGAG 2 cut(s) 524, 1063
HaeII RGCGCY 1 cut(s) 534
HgaI GACGC 5 cut(s) 203, 223, 418, 755, 859
HhaI GCGC 1 cut(s) 533
Hin1I GRCGYC 5 cut(s) 144, 195, 234, 747, 851
Hin1II CATG 3 cut(s) 730, 976, 1210
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 4 cut(s) 880, 1132, 1163, 1231
HphI GGTGA 6 cut(s) 169, 370, 469, 1141, 1146, 1273
Hpy166II GTNNAC 3 cut(s) 433, 754, 796
Hpy188I TCNGA 9 cut(s) 549, 567, 805, 885, 934, 994, 1137, 1162, 1236
Hpy188III TCNNGA 4 cut(s) 77, 503, 604, 781
Hpy8I GTNNAC 3 cut(s) 433, 754, 796
Hpy99I CGWCG 9 cut(s) 146, 149, 197, 239, 365, 397, 749, 823, 853
HpyAV CCTTC 4 cut(s) 229, 422, 817, 901
HpyCH4III ACNGT 4 cut(s) 85, 132, 1148, 1158
HpyCH4IV ACGT 6 cut(s) 144, 318, 392, 948, 1241, 1272
HpyCH4V TGCA 4 cut(s) 35, 424, 558, 655
HpyF10VI GCNNNNNNNGC 2 cut(s) 537, 655
HpyF3I CTNAG 2 cut(s) 645, 920
HpySE526I ACGT 6 cut(s) 144, 318, 392, 948, 1241, 1272
Hsp92I GRCGYC 5 cut(s) 144, 195, 234, 747, 851
Hsp92II CATG 3 cut(s) 730, 976, 1210
HspAI GCGC 1 cut(s) 531
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Ksp22I TGATCA 2 cut(s) 606, 885
LguI GCTCTTC 1 cut(s) 737
LmnI GCTCC 4 cut(s) 695, 773, 792, 845
Lsp1109I GCAGC 2 cut(s) 542, 667
LweI GCATC 3 cut(s) 180, 363, 567
MaeI CTAG 3 cut(s) 452, 552, 1247
MaeII ACGT 6 cut(s) 144, 318, 392, 948, 1241, 1272
MaeIII GTNAC 5 cut(s) 132, 475, 1073, 1142, 1152
MbiI CCGCTC 1 cut(s) 919
MboII GAAGA 7 cut(s) 349, 664, 724, 877, 916, 943, 1158
MflI RGATCY 2 cut(s) 544, 934
MhlI GDGCHC 2 cut(s) 770, 1050
MluCI AATT 5 cut(s) 36, 88, 124, 574, 1221
MmeI TCCRAC 2 cut(s) 339, 912
MreI CGCCGGCG 1 cut(s) 527
MroNI GCCGGC 1 cut(s) 527
MseI TTAA 1 cut(s) 1014
MspA1I CMGCKG 1 cut(s) 154
MvaI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1121
MvnI CGCG 3 cut(s) 863, 1034, 1052
MwoI GCNNNNNNNGC 2 cut(s) 537, 655
NaeI GCCGGC 1 cut(s) 529
NciI CCSGG 7 cut(s) 287, 791, 811, 927, 928, 981, 1202
NgoMIV GCCGGC 1 cut(s) 527
NlaIII CATG 3 cut(s) 730, 976, 1210
NlaIV GGNNCC 3 cut(s) 285, 691, 769
NmeAIII GCCGAG 1 cut(s) 566
NmuCI GTSAC 2 cut(s) 475, 1152
NspI RCATGY 1 cut(s) 730
PaqCI CACCTGC 1 cut(s) 640
PasI CCCWGGG 1 cut(s) 763
PceI AGGCCT 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 737
PcsI WCGNNNNNNNCGW 2 cut(s) 366, 741
PdiI GCCGGC 1 cut(s) 529
PfeI GAWTC 4 cut(s) 880, 1132, 1163, 1231
PflFI GACNNNGTC 2 cut(s) 143, 665
PflMI CCANNNNNTGG 1 cut(s) 1212
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 4 cut(s) 7, 557, 657, 1054
PpuMI RGGWCCY 3 cut(s) 208, 283, 373
Psp124BI GAGCTC 1 cut(s) 1050
Psp5II RGGWCCY 3 cut(s) 208, 283, 373
Psp6I CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1119
PspEI GGTNACC 1 cut(s) 1152
PspGI CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1119
PspN4I GGNNCC 3 cut(s) 285, 691, 769
PspPPI RGGWCCY 3 cut(s) 208, 283, 373
PsrI GAACNNNNNNTAC 2 cut(s) 1066, 1098
PsuI RGATCY 2 cut(s) 544, 934
PsyI GACNNNGTC 2 cut(s) 143, 665
RsaI GTAC 3 cut(s) 755, 872, 1037
RsaNI GTAC 3 cut(s) 754, 871, 1036
SacI GAGCTC 1 cut(s) 1050
SapI GCTCTTC 1 cut(s) 737
SaqAI TTAA 1 cut(s) 1014
SatI GCNGC 4 cut(s) 6, 556, 656, 1053
SduI GDGCHC 2 cut(s) 770, 1050
SfaNI GCATC 3 cut(s) 180, 363, 567
SgrAI CRCCGGYG 2 cut(s) 230, 527
SinI GGWCC 5 cut(s) 208, 277, 283, 373, 793
SmaI CCCGGG 1 cut(s) 928
Sse9I AATT 5 cut(s) 36, 88, 124, 574, 1221
SseBI AGGCCT 1 cut(s) 456
SspMI CTAG 3 cut(s) 452, 552, 1247
SstI GAGCTC 1 cut(s) 1050
StuI AGGCCT 1 cut(s) 456
StyI CCWWGG 3 cut(s) 204, 273, 1069
TaaI ACNGT 4 cut(s) 85, 132, 1148, 1158
TaiI ACGT 6 cut(s) 147, 321, 395, 951, 1244, 1275
TaqI TCGA 8 cut(s) 237, 459, 638, 660, 744, 821, 1044, 1109
TasI AATT 5 cut(s) 36, 88, 124, 574, 1221
TatI WGTACW 2 cut(s) 753, 870
TauI GCSGC 2 cut(s) 8, 1055
TfiI GAWTC 4 cut(s) 880, 1132, 1163, 1231
Tru1I TTAA 1 cut(s) 1014
Tru9I TTAA 1 cut(s) 1014
TseFI GTSAC 2 cut(s) 475, 1152
TseI GCWGC 2 cut(s) 555, 655
Tsp45I GTSAC 2 cut(s) 475, 1152
TspDTI ATGAA 4 cut(s) 58, 232, 989, 1280
TspGWI ACGGA 1 cut(s) 682
TspMI CCCGGG 1 cut(s) 926
Tth111I GACNNNGTC 2 cut(s) 143, 665
Van91I CCANNNNNTGG 1 cut(s) 1212
VpaK11BI GGWCC 5 cut(s) 208, 277, 283, 373, 793
XagI CCTNNNNNAGG 1 cut(s) 272
XapI RAATTY 1 cut(s) 88
XceI RCATGY 1 cut(s) 730
XmaI CCCGGG 1 cut(s) 926
XspI CTAG 3 cut(s) 452, 552, 1247
ZraI GACGTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.