MD06G1122000.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013309
Physical Location & Seq
Reverse (-)
26327316 .. 26330240
2925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1122000.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGGCAGCCACCACTAGCTTCCCCTACTCCTCTGCAATTTTCCTTTCAATTCTCATCATTTCAGGGTTCATGTTTCCAGAAACGGTAAATTCAATGGGGATAAACTACGGCCAAATCGCCAACAACTTACCCTCTCCCGACGACGTCGTTCCGCTGGTGAAAGCCATCGGCATCTCCAAGATCAAGCTCTACGACGCCGATCCCAAAGTCCTCAAAGCCTTCGCCAACACCGGCGTCCAGTTCATAGTGGGCCTCCCCAACGAGTACCTTGCCAAGGTCCAGGACCCGGCCCAGGCCCAATCCTGGGTCAAGTCCAATGTCCAGGCCCATCTCCCCGCCACCAACATTACCTGCATCTTCGTCGGCAATGAGGTCCTCACCTTCAACGACACGTCGTTGAGCAACAATCTCCTCCCGGCAATGCAGAGCGTCCACACCGCCCTTGTCGGTCTAGGCCTTGACAAGCAAGTCGCCGTCACCACCGCCCACTCCCTCTCCATTCTCCAGACCTCGTATCCGCCGTCCGCCGGCGCTTTCCGCCGAGATCTGACCGGCTGCATCACTCCGATCCTCAATTTCCACGCCAAAACCGGATCGCCGTTCCTGATCAATGCCTACCCTTACTTCGCTTACAAATCGAACCCTAAGCAAGTGCAGCTCGAGTACGTCCTCTTCCAGCCGAACTCCGGCTCCCTTGATCCTGCCACCAATCTCCACTACGACAACATGCTGTTCGCCCAGATCGACGCCGTGTACTCCGCCCTGGGCTCCCTCGGTTTCAAGAAGCTCCCGGTCCACATCTCCGAGACCGGGTGGCCGTCGAAGGGCGACGAGGACGAGGTCGGAGCGACGCCGGAGAACGCGAAGAAGTACAACGGTAATCTGATCAAGCTCATTTGCCAGAAGAAGGGCACGCCGCTCAGGCCCGGGTCGGATCTCAACATCTACGTGTTCGCCCTGTTCAATGAGAACATGAAACCCGGCCCGAGTTCAGAGAGGAACTTCGGGCTGTTTAAACCCGACGGGTCGCCCGCGTACTCAATCGGGCTCTCGACGGGCAACATTTCCTTTGGAAACTCTAATTCAAGTTCAAGTTCGGGTTCGGGTTCGGGTTCGGGCCAGGGACAGGTGAATCCGATTAGTAATGGTGGGTCGCCGTCTGGTTCTTCCGGCACGCCATACTTGTCCATTTCCTCCGCCCCCGCCACGATTCCATGTGATTGGTTGGATTGCGATGATGTGGCTGCTGGCCTCCTTGTTGATGAAAAGCTGATAGCAAAATATCTAAAGTTGCTCAAGATCAGATCTCAAAATCTGAATGGACGGTGGAAAAGAGAGAAAATAAATAGAGGAGGTTACTTGGGAATTAGGAACGAGGGCAATTATTATTTATTTATAATTCAGTGTCTTAATAACAACTCTAGTGGATTAGGGGTAGGAGGGTGA

Protein Analysis

482

Amino Acids

51.39

Weight (kDa)

8.34

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 32 - 348 1.7e-85 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1397
AasI GACNNNNNNGTC 1 cut(s) 467
AatII GACGTC 1 cut(s) 147
Acc36I ACCTGC 1 cut(s) 359
AccBSI CCGCTC 1 cut(s) 919
AccII CGCG 2 cut(s) 863, 1034
AclWI GGATC 5 cut(s) 194, 562, 601, 692, 942
AcoI YGGCCR 2 cut(s) 109, 815
AcsI RAATTY 1 cut(s) 88
AcyI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
AfaI GTAC 5 cut(s) 266, 665, 755, 872, 1037
AflIII ACRYGT 2 cut(s) 390, 948
AgsI TTSAA 7 cut(s) 48, 93, 385, 781, 964, 1086, 1092
AjiI CACGTC 1 cut(s) 393
AjnI CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1119
AloI GAACNNNNNNTCC 2 cut(s) 674, 706
AluBI AGCT 6 cut(s) 18, 187, 658, 787, 892, 1270
AluI AGCT 6 cut(s) 18, 187, 658, 787, 892, 1270
Alw26I GTCTC 1 cut(s) 800
AlwI GGATC 5 cut(s) 194, 562, 601, 692, 942
Ama87I CYCGRG 3 cut(s) 659, 926, 985
ApeKI GCWGC 4 cut(s) 5, 555, 655, 1244
ApoI RAATTY 1 cut(s) 88
AspLEI GCGC 1 cut(s) 533
AsuC2I CCSGG 7 cut(s) 287, 416, 791, 811, 927, 928, 981
AsuHPI GGTGA 4 cut(s) 169, 370, 469, 1141
AvaI CYCGRG 3 cut(s) 659, 926, 985
AvaII GGWCC 4 cut(s) 277, 283, 373, 793
BaeGI GKGCMC 1 cut(s) 914
BanII GRGCYC 2 cut(s) 770, 1050
BarI GAAGNNNNNNTAC 2 cut(s) 656, 688
BbvI GCAGC 4 cut(s) 17, 542, 667, 1231
BccI CCATC 2 cut(s) 173, 336
BceAI ACGGC 7 cut(s) 124, 458, 505, 583, 734, 802, 1141
BcgI CGANNNNNNTGC 4 cut(s) 251, 285, 343, 377
BciT130I CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1121
BciVI GTATCC 1 cut(s) 525
BclI TGATCA 2 cut(s) 606, 885
BcnI CCSGG 7 cut(s) 287, 416, 791, 811, 927, 928, 981
BcoDI GTCTC 1 cut(s) 800
BfaI CTAG 3 cut(s) 15, 452, 1422
BfoI RGCGCY 1 cut(s) 534
BfuAI ACCTGC 1 cut(s) 359
BfuI GTATCC 1 cut(s) 525
BglI GCCNNNNNGGC 1 cut(s) 922
BglII AGATCT 2 cut(s) 544, 1304
BisI GCNGC 5 cut(s) 6, 556, 656, 917, 1245
BlsI GCNGC 5 cut(s) 7, 557, 657, 918, 1246
Bme18I GGWCC 4 cut(s) 277, 283, 373, 793
BmeT110I CYCGRG 3 cut(s) 659, 926, 985
BmgBI CACGTC 1 cut(s) 393
BmiI GGNNCC 3 cut(s) 285, 691, 769
BmsI GCATC 3 cut(s) 180, 363, 567
BpmI CTGGAG 1 cut(s) 488
Bpu10I CCTNAGC 2 cut(s) 645, 920
BpuEI CTTGAG 1 cut(s) 1280
BpuMI CCSGG 7 cut(s) 287, 416, 791, 811, 927, 928, 981
BsaAI YACGTR 1 cut(s) 949
BsaHI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
BsaI GGTCTC 1 cut(s) 800
BsaJI CCNNGG 8 cut(s) 273, 291, 303, 762, 763, 772, 926, 1120
BsaWI WCCGGW 1 cut(s) 590
BsaXI ACNNNNNCTCC 4 cut(s) 479, 509, 674, 704
Bse118I RCCGGY 3 cut(s) 230, 527, 551
Bse1I ACTGG 1 cut(s) 238
Bse3DI GCAATG 2 cut(s) 373, 426
BseBI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1121
BseDI CCNNGG 8 cut(s) 273, 291, 303, 762, 763, 772, 926, 1120
BseMI GCAATG 2 cut(s) 373, 426
BseMII CTCAG 1 cut(s) 934
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 3 cut(s) 19, 401, 1365
BseSI GKGCMC 1 cut(s) 914
BseXI GCAGC 4 cut(s) 17, 542, 667, 1231
BsgI GTGCAG 1 cut(s) 674
Bsh1236I CGCG 2 cut(s) 863, 1034
BsiHKCI CYCGRG 3 cut(s) 659, 926, 985
BslFI GGGAC 1 cut(s) 1137
BsmAI GTCTC 1 cut(s) 800
BsmFI GGGAC 1 cut(s) 1137
Bso31I GGTCTC 1 cut(s) 800
BsoBI CYCGRG 3 cut(s) 659, 926, 985
Bsp1286I GDGCHC 3 cut(s) 770, 914, 1050
BspCNI CTCAG 1 cut(s) 933
BspFNI CGCG 2 cut(s) 863, 1034
BspLI GGNNCC 3 cut(s) 285, 691, 769
BspMI ACCTGC 1 cut(s) 359
BspPI GGATC 5 cut(s) 194, 562, 601, 692, 942
BspTNI GGTCTC 1 cut(s) 800
BsrBI CCGCTC 1 cut(s) 919
BsrDI GCAATG 2 cut(s) 373, 426
BsrFI RCCGGY 3 cut(s) 230, 527, 551
BsrI ACTGG 1 cut(s) 238
BssAI RCCGGY 3 cut(s) 230, 527, 551
BssECI CCNNGG 8 cut(s) 273, 291, 303, 762, 763, 772, 926, 1120
BssNI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1121
Bst4CI ACNGT 3 cut(s) 85, 878, 1326
Bst6I CTCTTC 1 cut(s) 677
BstACI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
BstBAI YACGTR 1 cut(s) 949
BstC8I GCNNGC 5 cut(s) 529, 914, 1032, 1175, 1249
BstDEI CTNAG 2 cut(s) 645, 920
BstENI CCTNNNNNAGG 1 cut(s) 272
BstFNI CGCG 2 cut(s) 863, 1034
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 1 cut(s) 533
BstMAI GTCTC 1 cut(s) 800
BstMWI GCNNNNNNNGC 3 cut(s) 537, 655, 922
BstNI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1121
BstNSI RCATGY 1 cut(s) 730
BstSLI GKGCMC 1 cut(s) 914
BstUI CGCG 2 cut(s) 863, 1034
BstV1I GCAGC 4 cut(s) 17, 542, 667, 1231
BstX2I RGATCY 3 cut(s) 544, 934, 1304
BstXI CCANNNNNNTGG 1 cut(s) 1221
BstYI RGATCY 3 cut(s) 544, 934, 1304
BsuI GTATCC 1 cut(s) 525
BtgZI GCGATG 1 cut(s) 1248
BtrI CACGTC 1 cut(s) 393
BtsIMutI CAGTG 1 cut(s) 1409
BveI ACCTGC 1 cut(s) 359
Cac8I GCNNGC 5 cut(s) 529, 914, 1032, 1175, 1249
CfoI GCGC 1 cut(s) 533
Cfr10I RCCGGY 3 cut(s) 230, 527, 551
Cfr9I CCCGGG 1 cut(s) 926
CseI GACGC 5 cut(s) 203, 223, 418, 755, 859
Csp6I GTAC 5 cut(s) 265, 664, 754, 871, 1036
CspCI CAANNNNNGTGG 2 cut(s) 1405, 1440
CviAII CATG 4 cut(s) 70, 727, 973, 1215
CviQI GTAC 5 cut(s) 265, 664, 754, 871, 1036
DdeI CTNAG 2 cut(s) 645, 920
DraI TTTAAA 1 cut(s) 1015
DrdI GACNNNNNNGTC 1 cut(s) 467
DseDI GACNNNNNNGTC 1 cut(s) 467
EaeI YGGCCR 2 cut(s) 109, 815
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
EciI GGCGGA 5 cut(s) 507, 514, 527, 748, 1186
Eco130I CCWWGG 1 cut(s) 273
Eco147I AGGCCT 1 cut(s) 456
Eco24I GRGCYC 2 cut(s) 770, 1050
Eco31I GGTCTC 1 cut(s) 800
Eco47I GGWCC 4 cut(s) 277, 283, 373, 793
Eco88I CYCGRG 3 cut(s) 659, 926, 985
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoO109I RGGNCCY 2 cut(s) 283, 373
EcoRII CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1119
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 2 cut(s) 770, 1050
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 4 cut(s) 73, 730, 976, 1218
FaiI YATR 7 cut(s) 71, 245, 728, 974, 1180, 1216, 1397
FaqI GGGAC 1 cut(s) 1137
FatI CATG 4 cut(s) 69, 726, 972, 1214
FauI CCCGC 3 cut(s) 343, 1039, 1210
FbaI TGATCA 2 cut(s) 606, 885
Fnu4HI GCNGC 5 cut(s) 6, 556, 656, 917, 1245
FriOI GRGCYC 2 cut(s) 770, 1050
Fsp4HI GCNGC 5 cut(s) 6, 556, 656, 917, 1245
FspBI CTAG 3 cut(s) 15, 452, 1422
GlaI GCGC 1 cut(s) 532
GluI GCNGC 5 cut(s) 6, 556, 656, 917, 1245
GsuI CTGGAG 1 cut(s) 488
HaeII RGCGCY 1 cut(s) 534
HgaI GACGC 5 cut(s) 203, 223, 418, 755, 859
HhaI GCGC 1 cut(s) 533
Hin1I GRCGYC 5 cut(s) 144, 195, 234, 747, 851
Hin1II CATG 4 cut(s) 73, 730, 976, 1218
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HinfI GANTC 2 cut(s) 1132, 1210
HphI GGTGA 4 cut(s) 169, 370, 469, 1141
Hpy166II GTNNAC 3 cut(s) 433, 754, 796
Hpy188III TCNNGA 7 cut(s) 77, 137, 505, 604, 781, 1051, 1297
Hpy8I GTNNAC 3 cut(s) 433, 754, 796
HpyAV CCTTC 4 cut(s) 229, 391, 817, 901
HpyCH4III ACNGT 3 cut(s) 85, 878, 1326
HpyCH4IV ACGT 4 cut(s) 144, 392, 666, 948
HpyCH4V TGCA 5 cut(s) 35, 354, 424, 558, 655
HpyF10VI GCNNNNNNNGC 3 cut(s) 537, 655, 922
HpyF3I CTNAG 2 cut(s) 645, 920
HpySE526I ACGT 4 cut(s) 144, 392, 666, 948
Hsp92I GRCGYC 5 cut(s) 144, 195, 234, 747, 851
Hsp92II CATG 4 cut(s) 73, 730, 976, 1218
HspAI GCGC 1 cut(s) 531
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Ksp22I TGATCA 2 cut(s) 606, 885
LmnI GCTCC 4 cut(s) 695, 773, 792, 845
Lsp1109I GCAGC 4 cut(s) 17, 542, 667, 1231
LweI GCATC 3 cut(s) 180, 363, 567
MaeI CTAG 3 cut(s) 15, 452, 1422
MaeII ACGT 4 cut(s) 144, 392, 666, 948
MaeIII GTNAC 2 cut(s) 475, 1355
MbiI CCGCTC 1 cut(s) 919
MboII GAAGA 5 cut(s) 349, 664, 877, 916, 1158
MflI RGATCY 3 cut(s) 544, 934, 1304
MhlI GDGCHC 3 cut(s) 770, 914, 1050
MluCI AATT 8 cut(s) 36, 48, 88, 574, 1081, 1365, 1381, 1398
MmeI TCCRAC 3 cut(s) 823, 912, 1206
MreI CGCCGGCG 1 cut(s) 527
MroNI GCCGGC 1 cut(s) 527
MseI TTAA 2 cut(s) 1014, 1410
MslI CAYNNNNRTG 1 cut(s) 947
MspA1I CMGCKG 1 cut(s) 154
MssI GTTTAAAC 1 cut(s) 1015
MvaI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1121
MvnI CGCG 2 cut(s) 863, 1034
MwoI GCNNNNNNNGC 3 cut(s) 537, 655, 922
NaeI GCCGGC 1 cut(s) 529
NciI CCSGG 7 cut(s) 287, 416, 791, 811, 927, 928, 981
NgoMIV GCCGGC 1 cut(s) 527
NlaIII CATG 4 cut(s) 73, 730, 976, 1218
NlaIV GGNNCC 3 cut(s) 285, 691, 769
NmeAIII GCCGAG 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 475
NspI RCATGY 1 cut(s) 730
PaeR7I CTCGAG 1 cut(s) 659
PasI CCCWGGG 1 cut(s) 763
PceI AGGCCT 1 cut(s) 456
PcsI WCGNNNNNNNCGW 2 cut(s) 518, 741
PdiI GCCGGC 1 cut(s) 529
PfeI GAWTC 2 cut(s) 1132, 1210
PflFI GACNNNGTC 2 cut(s) 143, 839
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 5 cut(s) 7, 557, 657, 918, 1246
PmeI GTTTAAAC 1 cut(s) 1015
Ppu21I YACGTR 1 cut(s) 949
PpuMI RGGWCCY 2 cut(s) 283, 373
PsiI TTATAA 1 cut(s) 1397
Psp5II RGGWCCY 2 cut(s) 283, 373
Psp6I CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1119
PspGI CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1119
PspN4I GGNNCC 3 cut(s) 285, 691, 769
PspPPI RGGWCCY 2 cut(s) 283, 373
PspXI VCTCGAGB 1 cut(s) 659
PsuI RGATCY 3 cut(s) 544, 934, 1304
PsyI GACNNNGTC 2 cut(s) 143, 839
RsaI GTAC 5 cut(s) 266, 665, 755, 872, 1037
RsaNI GTAC 5 cut(s) 265, 664, 754, 871, 1036
RseI CAYNNNNRTG 1 cut(s) 947
SaqAI TTAA 2 cut(s) 1014, 1410
SatI GCNGC 5 cut(s) 6, 556, 656, 917, 1245
SduI GDGCHC 3 cut(s) 770, 914, 1050
SfaNI GCATC 3 cut(s) 180, 363, 567
Sfr274I CTCGAG 1 cut(s) 659
SgrAI CRCCGGYG 2 cut(s) 230, 527
SinI GGWCC 4 cut(s) 277, 283, 373, 793
SlaI CTCGAG 1 cut(s) 659
SmaI CCCGGG 1 cut(s) 928
SmiMI CAYNNNNRTG 1 cut(s) 947
SmlI CTYRAG 2 cut(s) 659, 1295
SmoI CTYRAG 2 cut(s) 659, 1295
Sse9I AATT 8 cut(s) 36, 48, 88, 574, 1081, 1365, 1381, 1398
SseBI AGGCCT 1 cut(s) 456
SspMI CTAG 3 cut(s) 15, 452, 1422
StuI AGGCCT 1 cut(s) 456
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 3 cut(s) 85, 878, 1326
TaiI ACGT 4 cut(s) 147, 395, 669, 951
TaqI TCGA 5 cut(s) 638, 660, 744, 821, 1052
TaqII GACCGA 1 cut(s) 437
TasI AATT 8 cut(s) 36, 48, 88, 574, 1081, 1365, 1381, 1398
TatI WGTACW 2 cut(s) 753, 870
TauI GCSGC 1 cut(s) 919
TfiI GAWTC 2 cut(s) 1132, 1210
Tru1I TTAA 2 cut(s) 1014, 1410
Tru9I TTAA 2 cut(s) 1014, 1410
TscAI CASTG 1 cut(s) 1409
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 4 cut(s) 5, 555, 655, 1244
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 4 cut(s) 58, 232, 989, 1278
TspMI CCCGGG 1 cut(s) 926
TspRI CASTG 1 cut(s) 1409
Tth111I GACNNNGTC 2 cut(s) 143, 839
VpaK11BI GGWCC 4 cut(s) 277, 283, 373, 793
XagI CCTNNNNNAGG 1 cut(s) 272
XapI RAATTY 1 cut(s) 88
XceI RCATGY 1 cut(s) 730
XhoI CTCGAG 1 cut(s) 659
XmaI CCCGGG 1 cut(s) 926
XspI CTAG 3 cut(s) 15, 452, 1422
ZraI GACGTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.