FvH4_5g12060

DNA polymerase I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004379
Physical Location & Seq
Reverse (-)
6809235 .. 6811620
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12060.t3

Sequence Viewer

Length: 1278 bp
ATGAATTTCTCTTCTCTGCTACACTTTCAGTCCCTTTCCAGATGCTTCTACTTCTTTCGCCCAGCAGCAGCTCAGAGGCTTGGCCACCACTTGCGCACTCCCAAGAATCTCACTTCCCTCCATTTTCCTCCTGTAAAATCCGCAAAGGGATATTGTAATCTATCCACTAGTGTTCACTCAGCATTACCGGTACTTGTTCATGCCAATGGAGATTTGCTGATTGATTCAGGAAAGCTTGAAGAGAAAACAGTAAATACTAACCCTTCAAATGGTAGAGTAATGCTCATTGATGGCACATCCATCATTTACAGATCATATTACAAGCTTCTCGCCAAGTTACACCATGGCCACCTGACACATGCTGATGGAAATGGAGATTGGGTGCTGACCATATTTACGGCGCTTTCTCTTATAATTGATGTGTTAAAGTTTGTCCCTTCCCATGTGGCGGTGGTGTTTGACCACGATGGAGGTTCATTTGTTCAAACTGGTGTTTCATCCAAAGAGAGTTTTAGAGGAAAAGGCATGAACTTTCGTCATACTTTGTACCCCGCATACAAGAGCAATCGCCCCCCTACACCAGATACCATTGTTCAGGGACTTCAATACTTGAAAGCATCCCTCAAATCTATGTCCATCACAGTGATTGAGGTTCCAGGTGTGGAAGCTGATGATGTGATTGGAACATTGGCTGTTAGGAGTGTGGATAATGGTTACAAGGTACGGGTTGTCTCCCCAGACAAAGACTTTTTTCAGATCTTATCTCCTTCCTTGCGTCTTCTACGTATTGCTCCCCGTGGCTTTGAGATGGTTTCATTTGGAATGGAGGACTTTGCTAAAAAATATGGAACCTTGCAACCCTCTCAATTTGTTGATGTGATGTCACTTGTTGGTGACAAATGTGATAACATTCCAGGAGTTGATGGAATTGGGAACGTTCATGCTCTGCAACTCATAACTAAATTTGGTTCGTTGGAGAATTTATTGGAGAATGTGGATCAAGTTGAGGAGGAACGAATCAGAAAGGCCTTGGTAGCAGGTGCTGATCAGGCTCTTTTGAGCAAGAACTTGGCACTATTACGTTGTGATCTTCCATTCTACATGGTACCATTCAATACAAACGATCTGGCATTTACGAAACCTGAGGATGATGGAGAGAAATTCACGAGTCTCTTGACTGCCATTAGTGCATATGCGGAAGGGTTTTCTGCTGAGCCAGTCATAAGGAGAGCATTTTACTTATGGAACAAACTAGAAAACCATGACCAAGTTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_3_exonuc 5_3_exonuc_N

Protein Analysis

426

Amino Acids

47.08

Weight (kDa)

7.26

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_3_exonuc_N PF02739 93 - 160 2.8e-07 5'-3' exonuclease, N-terminal resolvase-like domain
5_3_exonuc_N PF02739 174 - 283 1.8e-28 5'-3' exonuclease, N-terminal resolvase-like domain
5_3_exonuc PF01367 286 - 382 2.5e-27 5'-3' exonuclease, C-terminal SAM fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 413
AarI CACCTGC 1 cut(s) 1028
Acc16I TGCGCA 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 1028
Acc65I GGTACC 1 cut(s) 1105
AccB1I GGYRCC 1 cut(s) 1105
AciI CCGC 4 cut(s) 141, 449, 552, 1196
AclI AACGTT 1 cut(s) 936
AclWI GGATC 1 cut(s) 1005
AcoI YGGCCR 2 cut(s) 82, 346
AcsI RAATTY 4 cut(s) 4, 962, 979, 1160
AfaI GTAC 4 cut(s) 192, 548, 723, 1107
AfiI CCNNNNNNNGG 2 cut(s) 269, 448
AgeI ACCGGT 1 cut(s) 187
AgsI TTSAA 6 cut(s) 239, 267, 485, 605, 613, 1114
AhlI ACTAGT 1 cut(s) 167
AjnI CCWGG 2 cut(s) 655, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 361, 393
AluBI AGCT 4 cut(s) 71, 235, 325, 668
AluI AGCT 4 cut(s) 71, 235, 325, 668
Alw26I GTCTC 2 cut(s) 736, 1175
AlwI GGATC 1 cut(s) 1005
AlwNI CAGNNNCTG 1 cut(s) 1043
AoxI GGCC 3 cut(s) 82, 346, 1026
ApeKI GCWGC 2 cut(s) 65, 68
ApoI RAATTY 4 cut(s) 4, 962, 979, 1160
AsiGI ACCGGT 1 cut(s) 187
Asp718I GGTACC 1 cut(s) 1105
AspLEI GCGC 2 cut(s) 96, 403
AsuHPI GGTGA 1 cut(s) 905
AxyI CCTNAGG 1 cut(s) 1143
BaeI ACNNNNGTAYC 2 cut(s) 576, 609
BalI TGGCCA 2 cut(s) 84, 348
BanI GGYRCC 1 cut(s) 1105
BauI CACGAG 1 cut(s) 1165
BbsI GAAGAC 1 cut(s) 770
BbvI GCAGC 2 cut(s) 77, 80
BccI CCATC 8 cut(s) 284, 308, 359, 461, 644, 802, 917, 1145
BceAI ACGGC 1 cut(s) 414
BciT130I CCWGG 2 cut(s) 657, 915
BclI TGATCA 1 cut(s) 1045
BcoDI GTCTC 2 cut(s) 736, 1175
BcuI ACTAGT 1 cut(s) 167
BfaI CTAG 3 cut(s) 168, 1253, 1276
BfoI RGCGCY 1 cut(s) 404
BfuAI ACCTGC 1 cut(s) 1028
BglII AGATCT 1 cut(s) 756
BisI GCNGC 2 cut(s) 66, 69
BlpI GCTNAGC 1 cut(s) 1212
BlsI GCNGC 2 cut(s) 67, 70
Bme1390I CCNGG 2 cut(s) 657, 915
BmiI GGNNCC 3 cut(s) 654, 850, 1107
BmrFI CCNGG 2 cut(s) 657, 915
BmsI GCATC 2 cut(s) 32, 626
BpiI GAAGAC 1 cut(s) 770
BplI GAGNNNNNCTC 2 cut(s) 267, 299
Bpu1102I GCTNAGC 1 cut(s) 1212
BsaAI YACGTR 1 cut(s) 785
BsaJI CCNNGG 3 cut(s) 343, 796, 1029
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 366, 396
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 448
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 2 cut(s) 493, 1217
Bse21I CCTNAGG 1 cut(s) 1143
BseBI CCWGG 2 cut(s) 657, 915
BseDI CCNNGG 3 cut(s) 343, 796, 1029
BseGI GGATG 4 cut(s) 296, 497, 617, 1153
BseLI CCNNNNNNNGG 2 cut(s) 269, 448
BseMII CTCAG 4 cut(s) 86, 192, 1134, 1203
BseNI ACTGG 2 cut(s) 493, 1217
BseRI GAGGAG 1 cut(s) 1022
BseXI GCAGC 2 cut(s) 77, 80
BseYI CCCAGC 1 cut(s) 61
BshFI GGCC 3 cut(s) 84, 348, 1028
BshNI GGYRCC 1 cut(s) 1105
BshTI ACCGGT 1 cut(s) 187
BsiSI CCGG 1 cut(s) 188
BslFI GGGAC 3 cut(s) 16, 419, 612
BslI CCNNNNNNNGG 2 cut(s) 269, 448
BsmAI GTCTC 2 cut(s) 736, 1175
BsmFI GGGAC 3 cut(s) 16, 419, 612
BsnI GGCC 3 cut(s) 84, 348, 1028
Bsp143I GATC 6 cut(s) 311, 756, 997, 1045, 1087, 1123
Bsp1720I GCTNAGC 1 cut(s) 1212
Bsp19I CCATGG 1 cut(s) 343
BspACI CCGC 4 cut(s) 141, 449, 552, 1196
BspANI GGCC 3 cut(s) 84, 348, 1028
BspCNI CTCAG 4 cut(s) 85, 191, 1135, 1204
BspLI GGNNCC 3 cut(s) 654, 850, 1107
BspMI ACCTGC 1 cut(s) 1028
BspPI GGATC 1 cut(s) 1005
BspT107I GGYRCC 1 cut(s) 1105
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 2 cut(s) 493, 1217
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 3 cut(s) 343, 796, 1029
BssMI GATC 6 cut(s) 311, 756, 997, 1045, 1087, 1123
BssSI CACGAG 1 cut(s) 1165
BssT1I CCWWGG 2 cut(s) 343, 1029
Bst2BI CACGAG 1 cut(s) 1165
Bst2UI CCWGG 2 cut(s) 657, 915
Bst4CI ACNGT 2 cut(s) 250, 643
Bst6I CTCTTC 2 cut(s) 16, 234
BstBAI YACGTR 1 cut(s) 785
BstDEI CTNAG 4 cut(s) 72, 178, 1143, 1212
BstDSI CCRYGG 2 cut(s) 343, 796
BstF5I GGATG 4 cut(s) 296, 497, 617, 1153
BstH2I RGCGCY 1 cut(s) 404
BstHHI GCGC 2 cut(s) 96, 403
BstKTI GATC 6 cut(s) 314, 759, 1000, 1048, 1090, 1126
BstMAI GTCTC 2 cut(s) 736, 1175
BstMBI GATC 6 cut(s) 311, 756, 997, 1045, 1087, 1123
BstMWI GCNNNNNNNGC 3 cut(s) 1034, 1049, 1187
BstNI CCWGG 2 cut(s) 657, 915
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 2 cut(s) 655, 913
BstSNI TACGTA 1 cut(s) 785
BstV1I GCAGC 2 cut(s) 77, 80
BstV2I GAAGAC 1 cut(s) 770
BstX2I RGATCY 1 cut(s) 756
BstYI RGATCY 1 cut(s) 756
Bsu36I CCTNAGG 1 cut(s) 1143
BsuRI GGCC 3 cut(s) 84, 348, 1028
BtgI CCRYGG 2 cut(s) 343, 796
BtsCI GGATG 4 cut(s) 296, 497, 617, 1153
BtsIMutI CAGTG 1 cut(s) 648
BveI ACCTGC 1 cut(s) 1028
CaiI CAGNNNCTG 1 cut(s) 1043
CfoI GCGC 2 cut(s) 96, 403
Cfr10I RCCGGY 1 cut(s) 187
CseI GACGC 1 cut(s) 764
Csp6I GTAC 4 cut(s) 191, 547, 722, 1106
CspAI ACCGGT 1 cut(s) 187
CviAII CATG 8 cut(s) 200, 344, 359, 443, 526, 941, 1102, 1262
CviQI GTAC 4 cut(s) 191, 547, 722, 1106
DdeI CTNAG 4 cut(s) 72, 178, 1143, 1212
DpnI GATC 6 cut(s) 313, 758, 999, 1047, 1089, 1125
DpnII GATC 6 cut(s) 311, 756, 997, 1045, 1087, 1123
EaeI YGGCCR 2 cut(s) 82, 346
Eam1104I CTCTTC 2 cut(s) 16, 234
EarI CTCTTC 2 cut(s) 16, 234
Eco105I TACGTA 1 cut(s) 785
Eco130I CCWWGG 2 cut(s) 343, 1029
Eco147I AGGCCT 1 cut(s) 1028
Eco81I CCTNAGG 1 cut(s) 1143
EcoRII CCWGG 2 cut(s) 655, 913
EcoT14I CCWWGG 2 cut(s) 343, 1029
ErhI CCWWGG 2 cut(s) 343, 1029
FaeI CATG 8 cut(s) 203, 347, 362, 446, 529, 944, 1105, 1265
FaqI GGGAC 3 cut(s) 16, 419, 612
FatI CATG 8 cut(s) 199, 343, 358, 442, 525, 940, 1101, 1261
FauI CCCGC 1 cut(s) 559
FauNDI CATATG 1 cut(s) 1192
FbaI TGATCA 1 cut(s) 1045
Fnu4HI GCNGC 2 cut(s) 66, 69
FokI GGATG 4 cut(s) 283, 484, 604, 1160
Fsp4HI GCNGC 2 cut(s) 66, 69
FspBI CTAG 3 cut(s) 168, 1253, 1276
FspI TGCGCA 1 cut(s) 95
GlaI GCGC 2 cut(s) 95, 402
GluI GCNGC 2 cut(s) 66, 69
GsaI CCCAGC 1 cut(s) 65
HaeII RGCGCY 1 cut(s) 404
HaeIII GGCC 3 cut(s) 84, 348, 1028
HapII CCGG 1 cut(s) 188
HgaI GACGC 1 cut(s) 764
HhaI GCGC 2 cut(s) 96, 403
Hin1II CATG 8 cut(s) 203, 347, 362, 446, 529, 944, 1105, 1265
Hin6I GCGC 2 cut(s) 94, 401
HinP1I GCGC 2 cut(s) 94, 401
HindIII AAGCTT 2 cut(s) 233, 323
HinfI GANTC 4 cut(s) 106, 224, 1017, 1168
HpaII CCGG 1 cut(s) 188
HphI GGTGA 1 cut(s) 905
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 3 cut(s) 75, 756, 1022
Hpy188III TCNNGA 4 cut(s) 39, 228, 1165, 1174
Hpy8I GTNNAC 1 cut(s) 175
HpyAV CCTTC 4 cut(s) 273, 447, 777, 1193
HpyCH4III ACNGT 2 cut(s) 250, 643
HpyCH4IV ACGT 3 cut(s) 784, 936, 1081
HpyCH4V TGCA 3 cut(s) 856, 949, 1190
HpyF10VI GCNNNNNNNGC 3 cut(s) 1034, 1049, 1187
HpyF3I CTNAG 4 cut(s) 72, 178, 1143, 1212
HpySE526I ACGT 3 cut(s) 784, 936, 1081
Hsp92II CATG 8 cut(s) 203, 347, 362, 446, 529, 944, 1105, 1265
HspAI GCGC 2 cut(s) 94, 401
KpnI GGTACC 1 cut(s) 1109
Ksp22I TGATCA 1 cut(s) 1045
Kzo9I GATC 6 cut(s) 311, 756, 997, 1045, 1087, 1123
LmnI GCTCC 1 cut(s) 796
Lsp1109I GCAGC 2 cut(s) 77, 80
LweI GCATC 2 cut(s) 32, 626
MaeI CTAG 3 cut(s) 168, 1253, 1276
MaeII ACGT 3 cut(s) 784, 936, 1081
MaeIII GTNAC 4 cut(s) 336, 713, 882, 893
MalI GATC 6 cut(s) 313, 758, 999, 1047, 1089, 1125
MboI GATC 6 cut(s) 311, 756, 997, 1045, 1087, 1123
MboII GAAGA 4 cut(s) 3, 251, 770, 1082
MflI RGATCY 1 cut(s) 756
MlsI TGGCCA 2 cut(s) 84, 348
MluCI AATT 7 cut(s) 4, 414, 866, 927, 962, 979, 1160
MluNI TGGCCA 2 cut(s) 84, 348
MlyI GAGTC 1 cut(s) 1177
MmeI TCCRAC 1 cut(s) 954
Mox20I TGGCCA 2 cut(s) 84, 348
MscI TGGCCA 2 cut(s) 84, 348
MseI TTAA 1 cut(s) 425
MslI CAYNNNNRTG 3 cut(s) 204, 363, 641
Msp20I TGGCCA 2 cut(s) 84, 348
MspI CCGG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 657, 915
MvaI CCWGG 2 cut(s) 657, 915
MwoI GCNNNNNNNGC 3 cut(s) 1034, 1049, 1187
NcoI CCATGG 1 cut(s) 343
NdeI CATATG 1 cut(s) 1192
NdeII GATC 6 cut(s) 311, 756, 997, 1045, 1087, 1123
NlaIII CATG 8 cut(s) 203, 347, 362, 446, 529, 944, 1105, 1265
NlaIV GGNNCC 3 cut(s) 654, 850, 1107
NmuCI GTSAC 2 cut(s) 882, 893
NsbI TGCGCA 1 cut(s) 95
NspI RCATGY 1 cut(s) 362
PaqCI CACCTGC 1 cut(s) 1028
PceI AGGCCT 1 cut(s) 1028
PfeI GAWTC 3 cut(s) 106, 224, 1017
PfoI TCCNGGA 1 cut(s) 913
PinAI ACCGGT 1 cut(s) 187
PkrI GCNGC 2 cut(s) 67, 70
PleI GAGTC 1 cut(s) 1176
PpsI GAGTC 1 cut(s) 1176
Ppu21I YACGTR 1 cut(s) 785
PsiI TTATAA 1 cut(s) 413
Psp1406I AACGTT 1 cut(s) 936
Psp6I CCWGG 2 cut(s) 655, 913
PspFI CCCAGC 1 cut(s) 61
PspGI CCWGG 2 cut(s) 655, 913
PspN4I GGNNCC 3 cut(s) 654, 850, 1107
PstNI CAGNNNCTG 1 cut(s) 1043
PsuI RGATCY 1 cut(s) 756
RsaI GTAC 4 cut(s) 192, 548, 723, 1107
RsaNI GTAC 4 cut(s) 191, 547, 722, 1106
RseI CAYNNNNRTG 3 cut(s) 204, 363, 641
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 2 cut(s) 66, 69
Sau3AI GATC 6 cut(s) 311, 756, 997, 1045, 1087, 1123
SchI GAGTC 1 cut(s) 1177
ScrFI CCNGG 2 cut(s) 657, 915
SfaNI GCATC 2 cut(s) 32, 626
SmiMI CAYNNNNRTG 3 cut(s) 204, 363, 641
SnaBI TACGTA 1 cut(s) 785
SpeI ACTAGT 1 cut(s) 167
Sse9I AATT 7 cut(s) 4, 414, 866, 927, 962, 979, 1160
SseBI AGGCCT 1 cut(s) 1028
SsiI CCGC 4 cut(s) 141, 449, 552, 1196
SspMI CTAG 3 cut(s) 168, 1253, 1276
StuI AGGCCT 1 cut(s) 1028
StyD4I CCNGG 2 cut(s) 655, 913
StyI CCWWGG 2 cut(s) 343, 1029
TaaI ACNGT 2 cut(s) 250, 643
TaiI ACGT 3 cut(s) 787, 939, 1084
TasI AATT 7 cut(s) 4, 414, 866, 927, 962, 979, 1160
TfiI GAWTC 3 cut(s) 106, 224, 1017
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 1 cut(s) 648
TseFI GTSAC 2 cut(s) 882, 893
TseI GCWGC 2 cut(s) 65, 68
Tsp45I GTSAC 2 cut(s) 882, 893
TspDTI ATGAA 7 cut(s) 17, 188, 465, 486, 542, 804, 929
TspRI CASTG 1 cut(s) 648
XapI RAATTY 4 cut(s) 4, 962, 979, 1160
XceI RCATGY 1 cut(s) 362
XspI CTAG 3 cut(s) 168, 1253, 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.