Rh4CG143300

DNA polymerase I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004379
Physical Location & Seq
Forward (+)
28745900 .. 28758379
12480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG143300.1

Sequence Viewer

Length: 1443 bp
ATGCCCGTTTTCAACCAAAAAAAAAAAATCTTATTTGGTGGCAAGTTAAAGCGTGCGGCGTCGTGTAAATTTCATTTGGGCGGGGGGATAGAGCTCGTGGTGTTGTTGGACATTGGAGAATGGAGAGCGAGCGAGTGTGAGATGGTTCATATCTTATTTATGGCCTGCTCTTTCTCTTCGCTGCTTCACTTTCAATCCCTTTGGAGAAGCTTCAAGTTGTTTGGCCCAGGAGCTCACAAGAGGCTCGGGCACCACTTGCGCACTCCCAACAAGCTCACTTGTCTCCATTTTTCTCCTATATCATCCGCAAAGGGACATTGTAATCTAGCCACCAGTGTTCACTCGGCATTACCTGGACTTGTTCATGACAATGGAGATTTGCTGGTTGATTCGGGGAAGCGTGAAGAGAAAATTGTATATAGTAACCCTTCAAATGGTAGAGTAATGCTCATTGACGGCACATCCATCATCTATAGATCATACTACAAGCTTCTTGCCAAGTTACACCATGGCCATCTGACACATGCTGATGGAAATGGAGATTGGGTGCTGACCATATTTACAGCGCTTTCTCTTATAATTGATGTGCTACAGTTTGTTCCTTCCCATGTGGCGGTGGTGTTTGACCATGATGGAGGTTCATTTGGTCAAACTAGTGTTTCATCCAAAGAAAGTGTTAGAGGAAAAGGCATGAATTTTCGTCATACTTTATACCCCGCATACAAGAGCAACCGCCCCCCTACACCAGATACCATTGTTCAGGGACTTCAGTACTTGAAAGCATCCCTCAAATCTATGTCCATCACGGTGATTGAGGTTCCAGGTGTGGAAGCTGATGATGTGATTGGAACATTGGCTGTTAGGAGTGTTGATAATGGTTACAAGGTACGAGTTGTCTCCCCAGACAAAGACTTTTTTCAGATCCTATCTCCTTCATTGCGTCTTCTACGAATTGCTTCACGTGGCTTTGAGATGGTTTCGTTTGGAATGGAGGACTTTGCTAAAAAATATGGAACCTTGCAACCCTCTCAGTTTGTTGATGTGATGTCACTTGTTGGTGACAAATGTGATAACATTCCAGGGGTTGATGGAATTGGAAACGTTCATGCTCTGCAACTCATCACTAAATTTGCCTCATTGGAGAATTTATTGCAGTGTGTGCATCAAGTTGAGGAAGAACGAATCAGAAAGGCCTTGATAGCAGGTGCTGAACAGGCTATATTAAGCAAGAACTTGGCACTATTACGTTCTGATCTTCCATTCTACATGGTACCATTCAATACAAAAGATCTGGCATTTATAAAACCTGAGGATGATGGAGAGAAATTCAGGAGTCTCTTGACTGCCATTAGTGCGTATGCGGAAGGATTTTCTGCTGATCCAGTCATAAGAAGAGCATTTTACTTATGGAACAAACTACAAAACCATAACCATGTTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_3_exonuc 5_3_exonuc_N

Protein Analysis

480

Amino Acids

53.19

Weight (kDa)

8.97

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_3_exonuc_N PF02739 148 - 215 3e-07 5'-3' exonuclease, N-terminal resolvase-like domain
5_3_exonuc_N PF02739 230 - 338 2e-28 5'-3' exonuclease, N-terminal resolvase-like domain
5_3_exonuc PF01367 341 - 436 2.9e-24 5'-3' exonuclease, C-terminal SAM fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 578, 1301
AarI CACCTGC 1 cut(s) 1193
Acc16I TGCGCA 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 1193
Acc65I GGTACC 1 cut(s) 1270
AccB1I GGYRCC 2 cut(s) 249, 1270
AciI CCGC 7 cut(s) 56, 81, 306, 614, 717, 733, 1361
AclI AACGTT 1 cut(s) 1101
AclWI GGATC 2 cut(s) 916, 1373
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 5 cut(s) 68, 694, 1127, 1144, 1325
AcuI CTGAAG 1 cut(s) 752
AcvI CACGTG 1 cut(s) 962
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 3 cut(s) 773, 888, 1272
AfeI AGCGCT 1 cut(s) 567
AfiI CCNNNNNNNGG 2 cut(s) 434, 613
AgsI TTSAA 6 cut(s) 13, 194, 214, 432, 778, 1279
AhlI ACTAGT 1 cut(s) 653
AjnI CCWGG 4 cut(s) 226, 352, 820, 1078
AjuI GAANNNNNNNTTGG 2 cut(s) 526, 558
AluBI AGCT 6 cut(s) 94, 210, 233, 274, 490, 833
AluI AGCT 6 cut(s) 94, 210, 233, 274, 490, 833
Alw21I GWGCWC 2 cut(s) 96, 235
Alw26I GTCTC 3 cut(s) 287, 901, 1340
AlwI GGATC 2 cut(s) 916, 1373
AlwNI CAGNNNCTG 1 cut(s) 1208
Ama87I CYCGRG 1 cut(s) 245
Aor51HI AGCGCT 1 cut(s) 567
AoxI GGCC 4 cut(s) 162, 223, 511, 1191
ApeKI GCWGC 1 cut(s) 181
ApoI RAATTY 5 cut(s) 68, 694, 1127, 1144, 1325
Asp700I GAANNNNTTC 1 cut(s) 955
Asp718I GGTACC 1 cut(s) 1270
AspLEI GCGC 2 cut(s) 261, 568
AspS9I GGNCC 1 cut(s) 224
AsuHPI GGTGA 2 cut(s) 820, 1070
AvaI CYCGRG 1 cut(s) 245
AxyI CCTNAGG 1 cut(s) 1308
BaeGI GKGCMC 1 cut(s) 252
BaeI ACNNNNGTAYC 2 cut(s) 741, 774
BalI TGGCCA 1 cut(s) 513
BanI GGYRCC 2 cut(s) 249, 1270
BanII GRGCYC 2 cut(s) 96, 235
BauI CACGAG 1 cut(s) 95
BbrPI CACGTG 1 cut(s) 962
BbsI GAAGAC 1 cut(s) 935
Bbv12I GWGCWC 2 cut(s) 96, 235
BbvI GCAGC 1 cut(s) 168
BccI CCATC 9 cut(s) 136, 473, 522, 524, 626, 809, 967, 1082, 1310
BceAI ACGGC 1 cut(s) 472
BciT130I CCWGG 4 cut(s) 228, 354, 822, 1080
BcoDI GTCTC 3 cut(s) 287, 901, 1340
BcuI ACTAGT 1 cut(s) 653
BfaI CTAG 3 cut(s) 326, 654, 1441
BfmI CTRYAG 2 cut(s) 472, 590
BfoI RGCGCY 1 cut(s) 569
BfuAI ACCTGC 1 cut(s) 1193
BglII AGATCT 1 cut(s) 1288
BisI GCNGC 2 cut(s) 57, 182
BlsI GCNGC 2 cut(s) 58, 183
BmcAI AGTACT 1 cut(s) 773
Bme1390I CCNGG 4 cut(s) 228, 354, 822, 1080
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 4 cut(s) 251, 819, 1015, 1272
BmrFI CCNGG 4 cut(s) 228, 354, 822, 1080
BmsI GCATC 2 cut(s) 791, 1171
BpiI GAAGAC 1 cut(s) 935
BplI GAGNNNNNCTC 2 cut(s) 432, 464
BsaAI YACGTR 1 cut(s) 962
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 226, 508, 1079
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 434, 613
Bse1I ACTGG 2 cut(s) 333, 1382
Bse21I CCTNAGG 1 cut(s) 1308
Bse3DI GCAATG 1 cut(s) 935
BseBI CCWGG 4 cut(s) 228, 354, 822, 1080
BseDI CCNNGG 3 cut(s) 226, 508, 1079
BseGI GGATG 5 cut(s) 302, 461, 662, 782, 1318
BseLI CCNNNNNNNGG 2 cut(s) 434, 613
BseMI GCAATG 1 cut(s) 935
BseMII CTCAG 2 cut(s) 1043, 1299
BseNI ACTGG 2 cut(s) 333, 1382
BseSI GKGCMC 1 cut(s) 252
BseXI GCAGC 1 cut(s) 168
BshFI GGCC 4 cut(s) 164, 225, 513, 1193
BshNI GGYRCC 2 cut(s) 249, 1270
BsiHKAI GWGCWC 2 cut(s) 96, 235
BsiHKCI CYCGRG 1 cut(s) 245
BslFI GGGAC 2 cut(s) 327, 777
BslI CCNNNNNNNGG 2 cut(s) 434, 613
BsmAI GTCTC 3 cut(s) 287, 901, 1340
BsmFI GGGAC 2 cut(s) 327, 777
BsnI GGCC 4 cut(s) 164, 225, 513, 1193
BsoBI CYCGRG 1 cut(s) 245
Bsp1286I GDGCHC 3 cut(s) 96, 235, 252
Bsp143I GATC 5 cut(s) 476, 921, 1252, 1288, 1378
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 7 cut(s) 56, 81, 306, 614, 717, 733, 1361
BspANI GGCC 4 cut(s) 164, 225, 513, 1193
BspCNI CTCAG 2 cut(s) 1042, 1300
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 4 cut(s) 251, 819, 1015, 1272
BspMI ACCTGC 1 cut(s) 1193
BspPI GGATC 2 cut(s) 916, 1373
BspQI GCTCTTC 1 cut(s) 1387
BspT107I GGYRCC 2 cut(s) 249, 1270
BsrDI GCAATG 1 cut(s) 935
BsrI ACTGG 2 cut(s) 333, 1382
BssECI CCNNGG 3 cut(s) 226, 508, 1079
BssMI GATC 5 cut(s) 476, 921, 1252, 1288, 1378
BssNI GRCGYC 1 cut(s) 59
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 508
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 4 cut(s) 228, 354, 822, 1080
Bst4CI ACNGT 2 cut(s) 594, 808
Bst6I CTCTTC 3 cut(s) 181, 399, 1387
BstACI GRCGYC 1 cut(s) 59
BstAPI GCANNNNNTGC 2 cut(s) 256, 1159
BstBAI YACGTR 1 cut(s) 962
BstC8I GCNNGC 3 cut(s) 54, 130, 166
BstDEI CTNAG 2 cut(s) 1029, 1308
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 5 cut(s) 302, 461, 662, 782, 1318
BstH2I RGCGCY 1 cut(s) 569
BstHHI GCGC 2 cut(s) 261, 568
BstKTI GATC 5 cut(s) 479, 924, 1255, 1291, 1381
BstMAI GTCTC 3 cut(s) 287, 901, 1340
BstMBI GATC 5 cut(s) 476, 921, 1252, 1288, 1378
BstMWI GCNNNNNNNGC 5 cut(s) 256, 1159, 1199, 1214, 1352
BstNI CCWGG 4 cut(s) 228, 354, 822, 1080
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 4 cut(s) 226, 352, 820, 1078
BstSFI CTRYAG 2 cut(s) 472, 590
BstSLI GKGCMC 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 168
BstV2I GAAGAC 1 cut(s) 935
BstX2I RGATCY 2 cut(s) 921, 1288
BstYI RGATCY 2 cut(s) 921, 1288
Bsu36I CCTNAGG 1 cut(s) 1308
BsuRI GGCC 4 cut(s) 164, 225, 513, 1193
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 5 cut(s) 302, 461, 662, 782, 1318
BtsI GCAGTG 1 cut(s) 1160
BtsIMutI CAGTG 2 cut(s) 340, 1160
BveI ACCTGC 1 cut(s) 1193
Cac8I GCNNGC 3 cut(s) 54, 130, 166
CaiI CAGNNNCTG 1 cut(s) 1208
CciI TCATGA 1 cut(s) 364
CfoI GCGC 2 cut(s) 261, 568
Cfr13I GGNCC 1 cut(s) 224
CseI GACGC 2 cut(s) 48, 929
Csp6I GTAC 3 cut(s) 772, 887, 1271
CviAII CATG 9 cut(s) 365, 509, 524, 608, 629, 691, 1106, 1267, 1433
CviQI GTAC 3 cut(s) 772, 887, 1271
DdeI CTNAG 2 cut(s) 1029, 1308
DpnI GATC 5 cut(s) 478, 923, 1254, 1290, 1380
DpnII GATC 5 cut(s) 476, 921, 1252, 1288, 1378
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 3 cut(s) 181, 399, 1387
EarI CTCTTC 3 cut(s) 181, 399, 1387
Ecl136II GAGCTC 2 cut(s) 94, 233
Eco130I CCWWGG 1 cut(s) 508
Eco147I AGGCCT 1 cut(s) 1193
Eco24I GRGCYC 2 cut(s) 96, 235
Eco47III AGCGCT 1 cut(s) 567
Eco53kI GAGCTC 2 cut(s) 94, 233
Eco57I CTGAAG 1 cut(s) 752
Eco72I CACGTG 1 cut(s) 962
Eco81I CCTNAGG 1 cut(s) 1308
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 2 cut(s) 94, 233
EcoRII CCWGG 4 cut(s) 226, 352, 820, 1078
EcoT14I CCWWGG 1 cut(s) 508
EcoT38I GRGCYC 2 cut(s) 96, 235
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 9 cut(s) 368, 512, 527, 611, 632, 694, 1109, 1270, 1436
FaqI GGGAC 2 cut(s) 327, 777
FatI CATG 9 cut(s) 364, 508, 523, 607, 628, 690, 1105, 1266, 1432
FauI CCCGC 2 cut(s) 74, 724
Fnu4HI GCNGC 2 cut(s) 57, 182
FokI GGATG 5 cut(s) 289, 448, 649, 769, 1325
FriOI GRGCYC 2 cut(s) 96, 235
Fsp4HI GCNGC 2 cut(s) 57, 182
FspBI CTAG 3 cut(s) 326, 654, 1441
FspI TGCGCA 1 cut(s) 260
GlaI GCGC 2 cut(s) 260, 567
GluI GCNGC 2 cut(s) 57, 182
HaeII RGCGCY 1 cut(s) 569
HaeIII GGCC 4 cut(s) 164, 225, 513, 1193
HgaI GACGC 2 cut(s) 48, 929
HhaI GCGC 2 cut(s) 261, 568
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 9 cut(s) 368, 512, 527, 611, 632, 694, 1109, 1270, 1436
Hin6I GCGC 2 cut(s) 259, 566
HinP1I GCGC 2 cut(s) 259, 566
HindIII AAGCTT 2 cut(s) 208, 488
HinfI GANTC 3 cut(s) 389, 1182, 1333
HphI GGTGA 2 cut(s) 820, 1070
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 4 cut(s) 519, 921, 1187, 1252
Hpy188III TCNNGA 3 cut(s) 365, 1330, 1339
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 4 cut(s) 438, 612, 942, 1358
HpyCH4III ACNGT 2 cut(s) 594, 808
HpyCH4IV ACGT 3 cut(s) 961, 1101, 1246
HpyCH4V TGCA 4 cut(s) 1021, 1114, 1153, 1162
HpyF10VI GCNNNNNNNGC 5 cut(s) 256, 1159, 1199, 1214, 1352
HpyF3I CTNAG 2 cut(s) 1029, 1308
HpySE526I ACGT 3 cut(s) 961, 1101, 1246
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 9 cut(s) 368, 512, 527, 611, 632, 694, 1109, 1270, 1436
HspAI GCGC 2 cut(s) 259, 566
KpnI GGTACC 1 cut(s) 1274
Kzo9I GATC 5 cut(s) 476, 921, 1252, 1288, 1378
LguI GCTCTTC 1 cut(s) 1387
LmnI GCTCC 1 cut(s) 230
Lsp1109I GCAGC 1 cut(s) 168
LweI GCATC 2 cut(s) 791, 1171
MaeI CTAG 3 cut(s) 326, 654, 1441
MaeII ACGT 3 cut(s) 961, 1101, 1246
MaeIII GTNAC 5 cut(s) 422, 501, 878, 1047, 1058
MalI GATC 5 cut(s) 478, 923, 1254, 1290, 1380
MboI GATC 5 cut(s) 476, 921, 1252, 1288, 1378
MboII GAAGA 6 cut(s) 168, 416, 935, 1187, 1247, 1404
MflI RGATCY 2 cut(s) 921, 1288
MhlI GDGCHC 3 cut(s) 96, 235, 252
MlsI TGGCCA 1 cut(s) 513
MluCI AATT 9 cut(s) 68, 411, 579, 694, 951, 1092, 1127, 1144, 1325
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 1 cut(s) 1342
MmeI TCCRAC 1 cut(s) 87
Mox20I TGGCCA 1 cut(s) 513
MroXI GAANNNNTTC 1 cut(s) 955
MscI TGGCCA 1 cut(s) 513
MseI TTAA 2 cut(s) 47, 1223
MslI CAYNNNNRTG 4 cut(s) 369, 528, 806, 1431
Msp20I TGGCCA 1 cut(s) 513
MspR9I CCNGG 4 cut(s) 228, 354, 822, 1080
MvaI CCWGG 4 cut(s) 228, 354, 822, 1080
MwoI GCNNNNNNNGC 5 cut(s) 256, 1159, 1199, 1214, 1352
NcoI CCATGG 1 cut(s) 508
NdeII GATC 5 cut(s) 476, 921, 1252, 1288, 1378
NlaIII CATG 9 cut(s) 368, 512, 527, 611, 632, 694, 1109, 1270, 1436
NlaIV GGNNCC 4 cut(s) 251, 819, 1015, 1272
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 2 cut(s) 1047, 1058
NsbI TGCGCA 1 cut(s) 260
NspI RCATGY 1 cut(s) 527
PagI TCATGA 1 cut(s) 364
PaqCI CACCTGC 1 cut(s) 1193
PceI AGGCCT 1 cut(s) 1193
PciSI GCTCTTC 1 cut(s) 1387
PdmI GAANNNNTTC 1 cut(s) 955
PfeI GAWTC 2 cut(s) 389, 1182
PkrI GCNGC 2 cut(s) 58, 183
PleI GAGTC 1 cut(s) 1341
PmaCI CACGTG 1 cut(s) 962
PmlI CACGTG 1 cut(s) 962
PpsI GAGTC 1 cut(s) 1341
Ppu21I YACGTR 1 cut(s) 962
PsiI TTATAA 2 cut(s) 578, 1301
Psp124BI GAGCTC 2 cut(s) 96, 235
Psp1406I AACGTT 1 cut(s) 1101
Psp6I CCWGG 4 cut(s) 226, 352, 820, 1078
PspCI CACGTG 1 cut(s) 962
PspGI CCWGG 4 cut(s) 226, 352, 820, 1078
PspN4I GGNNCC 4 cut(s) 251, 819, 1015, 1272
PspPI GGNCC 1 cut(s) 224
PstNI CAGNNNCTG 1 cut(s) 1208
PsuI RGATCY 2 cut(s) 921, 1288
RsaI GTAC 3 cut(s) 773, 888, 1272
RsaNI GTAC 3 cut(s) 772, 887, 1271
RseI CAYNNNNRTG 4 cut(s) 369, 528, 806, 1431
SacI GAGCTC 2 cut(s) 96, 235
SapI GCTCTTC 1 cut(s) 1387
SaqAI TTAA 2 cut(s) 47, 1223
SatI GCNGC 2 cut(s) 57, 182
Sau3AI GATC 5 cut(s) 476, 921, 1252, 1288, 1378
Sau96I GGNCC 1 cut(s) 224
ScaI AGTACT 1 cut(s) 773
SchI GAGTC 1 cut(s) 1342
ScrFI CCNGG 4 cut(s) 228, 354, 822, 1080
SduI GDGCHC 3 cut(s) 96, 235, 252
SfaNI GCATC 2 cut(s) 791, 1171
SfcI CTRYAG 2 cut(s) 472, 590
SmiMI CAYNNNNRTG 4 cut(s) 369, 528, 806, 1431
SpeI ACTAGT 1 cut(s) 653
Sse9I AATT 9 cut(s) 68, 411, 579, 694, 951, 1092, 1127, 1144, 1325
SseBI AGGCCT 1 cut(s) 1193
SsiI CCGC 7 cut(s) 56, 81, 306, 614, 717, 733, 1361
SspMI CTAG 3 cut(s) 326, 654, 1441
SstI GAGCTC 2 cut(s) 96, 235
StuI AGGCCT 1 cut(s) 1193
StyD4I CCNGG 4 cut(s) 226, 352, 820, 1078
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 2 cut(s) 594, 808
TaiI ACGT 3 cut(s) 964, 1104, 1249
TasI AATT 9 cut(s) 68, 411, 579, 694, 951, 1092, 1127, 1144, 1325
TatI WGTACW 1 cut(s) 771
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 2 cut(s) 389, 1182
Tru1I TTAA 2 cut(s) 47, 1223
Tru9I TTAA 2 cut(s) 47, 1223
TscAI CASTG 2 cut(s) 340, 1160
TseFI GTSAC 2 cut(s) 1047, 1058
TseI GCWGC 1 cut(s) 181
Tsp45I GTSAC 2 cut(s) 1047, 1058
TspDTI ATGAA 8 cut(s) 62, 137, 353, 630, 651, 707, 924, 1094
TspRI CASTG 2 cut(s) 340, 1160
XapI RAATTY 5 cut(s) 68, 694, 1127, 1144, 1325
XceI RCATGY 1 cut(s) 527
XmnI GAANNNNTTC 1 cut(s) 955
XspI CTAG 3 cut(s) 326, 654, 1441
ZrmI AGTACT 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.