Prupe.1G206500_v2.0.a1

DNA polymerase I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0004379
Physical Location & Seq
Reverse (-)
20961056 .. 20974331
13276 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G206500.2

Sequence Viewer

Length: 1332 bp
ATGAATGTGAAATGGTGCTATGAATTCATGGCCTCTTCTCACTCATCGCTGCTTCACATTCATTCCTTTTCCCTTCGGAGAAGCTTCAGATTATTTGCCAGAAGCTTCTCAAGCATTCAGAGTCCAAAGAAACTTGATTCACTCCGCTTTTCTCCAATTAAATCCCCAAAGGGCTACTGTAACATATCCTGTAGTTTTAATTCGGCTTTACCGGGAGTTGTTCGTGAGAATGGGGATGCTGCATTTTACTCTAAAAGTGAGCAAGTACTATGCGGAGATATGCCGCTAGGTTCAGTCAAGCAAGAAGAGAAAACAGTGAACAGTAACCCTTCAGATGGTAGAGTCATGCTCATCGATGGCACATCAATCATTTATAGAGCGTACTATAAACTTTTAGCAAAGTTGCACCATGGCCATCTGTCACATGCTGATGGAAACGGAGATTGGGTGCTAACCATCTTTTCAGCACTTTCTCTTATAATTGATGTGCTGAAGTTTACCCCTTCCCATGTGGCGGTGGTGTTTGACCATGATGGAGTTTCATTTGGTCAAACTTGTATTTCATCCAAAGAAAATGTTAAAGGAAAAGGGCTGAATTTTCGTCATACTTTATACCCTGCATACAAGAGCAATCGCCCCCCTACACCTGATACCATTGTCCAAGGCCTTCAATACTTGAAAGCTTCCATTAAGGCTATGTCCATTAAGGTGATTGAGGTGCCAGGTGTGGAAGCTGATGATGTGATTGGAACGTTGGCTGTTAGGAGTGTTGATACTGGGTACAAGGTACGGGTTGTCTCCCCAGACAAAGACTTTTTCCAGATCCTATCTCCTTCATTACGTCTTTTACGAATTGCTCCACGTGGCTTTGATATGGTTTCATTTGGCATGGAAGAATTTGCTATAAAATATGGAAGCCTTCAACCTTCTCAGTTTGTTGATGTGATCTCACTTGTTGGTGACAAATGCGATAATATTCCAGGAGTTGATGGAATCGGAAACGTTCATGCTGTGCAACTCATCACTAAATTTGGCACATTGGAGAATCTTTTGCACTGTGTTGATCAAGTTGAAGAGGAACGAATCAGAAAGATCTTGATAACAAGTGCTGATCAGGCTGTCTTGAGCAAGAACTTGGCACTATTACGTTCTGATCTTCCATTCTACATGGTACCTTTCACCACAAATGATCTGGCATTTCAAAAGCCTGAGGACAATGGGGAGAAATTCACGAGCCTCTTAACGGCTATAAGTGCATATGCGGAAGGATTTTCAGCTGATCCAATCATCAGGAGAGCATTTTACTTATGGAACAAACTAGGAAAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_3_exonuc 5_3_exonuc_N

Protein Analysis

444

Amino Acids

49.11

Weight (kDa)

8.66

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 479
Acc65I GGTACC 1 cut(s) 1171
AccB1I GGYRCC 2 cut(s) 718, 1171
AciI CCGC 5 cut(s) 145, 273, 284, 515, 1262
AclI AACGTT 2 cut(s) 752, 1002
AclWI GGATC 2 cut(s) 817, 1274
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 5 cut(s) 23, 595, 896, 1028, 1226
AcuI CTGAAG 3 cut(s) 70, 315, 512
AcvI CACGTG 1 cut(s) 863
AfaI GTAC 5 cut(s) 267, 383, 782, 789, 1173
AfiI CCNNNNNNNGG 3 cut(s) 335, 514, 1243
AgsI TTSAA 5 cut(s) 671, 679, 923, 1073, 1202
AjnI CCWGG 2 cut(s) 721, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 427, 459
AluBI AGCT 5 cut(s) 84, 105, 683, 734, 1277
AluI AGCT 5 cut(s) 84, 105, 683, 734, 1277
Alw26I GTCTC 1 cut(s) 802
AlwI GGATC 2 cut(s) 817, 1274
AoxI GGCC 3 cut(s) 30, 412, 664
ApeKI GCWGC 2 cut(s) 49, 239
ApoI RAATTY 5 cut(s) 23, 595, 896, 1028, 1226
Asp718I GGTACC 1 cut(s) 1171
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 3 cut(s) 721, 971, 1171
AxyI CCTNAGG 1 cut(s) 1209
BaeI ACNNNNGTAYC 2 cut(s) 642, 675
BalI TGGCCA 1 cut(s) 414
BanI GGYRCC 2 cut(s) 718, 1171
BauI CACGAG 1 cut(s) 1231
BbrPI CACGTG 1 cut(s) 863
BbvI GCAGC 2 cut(s) 36, 226
BccI CCATC 7 cut(s) 329, 350, 423, 425, 464, 527, 983
BceAI ACGGC 1 cut(s) 1260
BciT130I CCWGG 2 cut(s) 723, 981
BclI TGATCA 2 cut(s) 1063, 1111
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 802
BfaI CTAG 2 cut(s) 287, 1319
BfmI CTRYAG 1 cut(s) 190
BglII AGATCT 1 cut(s) 1092
BisI GCNGC 3 cut(s) 50, 240, 284
BlsI GCNGC 3 cut(s) 51, 241, 285
BmcAI AGTACT 1 cut(s) 267
Bme1390I CCNGG 3 cut(s) 213, 723, 981
BmiI GGNNCC 2 cut(s) 720, 1173
BmrFI CCNGG 3 cut(s) 213, 723, 981
BmrI ACTGGG 1 cut(s) 786
BmsI GCATC 1 cut(s) 226
BmuI ACTGGG 1 cut(s) 786
BplI GAGNNNNNCTC 2 cut(s) 333, 365
BpuEI CTTGAG 2 cut(s) 94, 1144
BpuMI CCSGG 1 cut(s) 213
Bsa29I ATCGAT 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 863
BsaJI CCNNGG 2 cut(s) 409, 661
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 3 cut(s) 335, 514, 1243
Bse1I ACTGG 1 cut(s) 781
Bse21I CCTNAGG 1 cut(s) 1209
BseBI CCWGG 2 cut(s) 723, 981
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 2 cut(s) 409, 661
BseGI GGATG 2 cut(s) 241, 563
BseLI CCNNNNNNNGG 3 cut(s) 335, 514, 1243
BseMII CTCAG 2 cut(s) 944, 1200
BseNI ACTGG 1 cut(s) 781
BseXI GCAGC 2 cut(s) 36, 226
BshFI GGCC 3 cut(s) 32, 414, 666
BshNI GGYRCC 2 cut(s) 718, 1171
BshVI ATCGAT 1 cut(s) 354
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 3 cut(s) 335, 514, 1243
BsmAI GTCTC 1 cut(s) 802
BsmI GAATGC 1 cut(s) 114
BsnI GGCC 3 cut(s) 32, 414, 666
Bsp143I GATC 8 cut(s) 822, 945, 1063, 1092, 1111, 1153, 1189, 1279
Bsp19I CCATGG 1 cut(s) 409
BspACI CCGC 5 cut(s) 145, 273, 284, 515, 1262
BspANI GGCC 3 cut(s) 32, 414, 666
BspCNI CTCAG 2 cut(s) 943, 1201
BspDI ATCGAT 1 cut(s) 354
BspLI GGNNCC 2 cut(s) 720, 1173
BspPI GGATC 2 cut(s) 817, 1274
BspT107I GGYRCC 2 cut(s) 718, 1171
BsrI ACTGG 1 cut(s) 781
BssECI CCNNGG 2 cut(s) 409, 661
BssMI GATC 8 cut(s) 822, 945, 1063, 1092, 1111, 1153, 1189, 1279
BssSI CACGAG 1 cut(s) 1231
BssT1I CCWWGG 2 cut(s) 409, 661
Bst2BI CACGAG 1 cut(s) 1231
Bst2UI CCWGG 2 cut(s) 723, 981
Bst4CI ACNGT 4 cut(s) 179, 316, 323, 1058
Bst6I CTCTTC 3 cut(s) 40, 300, 1068
BstBAI YACGTR 1 cut(s) 863
BstDEI CTNAG 2 cut(s) 930, 1209
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 2 cut(s) 241, 563
BstKTI GATC 8 cut(s) 825, 948, 1066, 1095, 1114, 1156, 1192, 1282
BstMAI GTCTC 1 cut(s) 802
BstMBI GATC 8 cut(s) 822, 945, 1063, 1092, 1111, 1153, 1189, 1279
BstMWI GCNNNNNNNGC 3 cut(s) 111, 1115, 1253
BstNI CCWGG 2 cut(s) 723, 981
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 3 cut(s) 211, 721, 979
BstSFI CTRYAG 1 cut(s) 190
BstV1I GCAGC 2 cut(s) 36, 226
BstX2I RGATCY 2 cut(s) 822, 1092
BstYI RGATCY 2 cut(s) 822, 1092
Bsu15I ATCGAT 1 cut(s) 354
Bsu36I CCTNAGG 1 cut(s) 1209
BsuRI GGCC 3 cut(s) 32, 414, 666
BsuTUI ATCGAT 1 cut(s) 354
BtgI CCRYGG 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 2 cut(s) 241, 563
BtsIMutI CAGTG 2 cut(s) 321, 1054
ClaI ATCGAT 1 cut(s) 354
Csp6I GTAC 5 cut(s) 266, 382, 781, 788, 1172
CviAII CATG 9 cut(s) 28, 346, 410, 425, 509, 530, 889, 1007, 1168
CviQI GTAC 5 cut(s) 266, 382, 781, 788, 1172
DdeI CTNAG 2 cut(s) 930, 1209
DpnI GATC 8 cut(s) 824, 947, 1065, 1094, 1113, 1155, 1191, 1281
DpnII GATC 8 cut(s) 822, 945, 1063, 1092, 1111, 1153, 1189, 1279
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 3 cut(s) 40, 300, 1068
EarI CTCTTC 3 cut(s) 40, 300, 1068
Eco130I CCWWGG 2 cut(s) 409, 661
Eco147I AGGCCT 1 cut(s) 666
Eco57I CTGAAG 3 cut(s) 70, 315, 512
Eco72I CACGTG 1 cut(s) 863
Eco81I CCTNAGG 1 cut(s) 1209
EcoRI GAATTC 1 cut(s) 23
EcoRII CCWGG 2 cut(s) 721, 979
EcoT14I CCWWGG 2 cut(s) 409, 661
ErhI CCWWGG 2 cut(s) 409, 661
FaeI CATG 9 cut(s) 31, 349, 413, 428, 512, 533, 892, 1010, 1171
FatI CATG 9 cut(s) 27, 345, 409, 424, 508, 529, 888, 1006, 1167
FauNDI CATATG 1 cut(s) 1258
FbaI TGATCA 2 cut(s) 1063, 1111
Fnu4HI GCNGC 3 cut(s) 50, 240, 284
FokI GGATG 2 cut(s) 248, 550
Fsp4HI GCNGC 3 cut(s) 50, 240, 284
FspBI CTAG 2 cut(s) 287, 1319
GluI GCNGC 3 cut(s) 50, 240, 284
HaeIII GGCC 3 cut(s) 32, 414, 666
HapII CCGG 1 cut(s) 212
Hin1II CATG 9 cut(s) 31, 349, 413, 428, 512, 533, 892, 1010, 1171
HindIII AAGCTT 3 cut(s) 82, 103, 681
HinfI GANTC 6 cut(s) 121, 137, 342, 993, 1045, 1083
HpaII CCGG 1 cut(s) 212
HphI GGTGA 3 cut(s) 721, 971, 1171
Hpy166II GTNNAC 2 cut(s) 319, 498
Hpy188I TCNGA 7 cut(s) 78, 89, 120, 334, 998, 1088, 1153
Hpy188III TCNNGA 6 cut(s) 224, 820, 1096, 1123, 1231, 1291
Hpy8I GTNNAC 2 cut(s) 319, 498
HpyAV CCTTC 8 cut(s) 83, 339, 513, 677, 843, 929, 936, 1259
HpyCH4III ACNGT 4 cut(s) 179, 316, 323, 1058
HpyCH4IV ACGT 5 cut(s) 752, 841, 862, 1002, 1147
HpyCH4V TGCA 6 cut(s) 242, 406, 620, 1015, 1054, 1256
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 1115, 1253
HpyF3I CTNAG 2 cut(s) 930, 1209
HpySE526I ACGT 5 cut(s) 752, 841, 862, 1002, 1147
Hsp92II CATG 9 cut(s) 31, 349, 413, 428, 512, 533, 892, 1010, 1171
KpnI GGTACC 1 cut(s) 1175
Ksp22I TGATCA 2 cut(s) 1063, 1111
Kzo9I GATC 8 cut(s) 822, 945, 1063, 1092, 1111, 1153, 1189, 1279
LmnI GCTCC 1 cut(s) 862
Lsp1109I GCAGC 2 cut(s) 36, 226
LweI GCATC 1 cut(s) 226
MaeI CTAG 2 cut(s) 287, 1319
MaeII ACGT 5 cut(s) 752, 841, 862, 1002, 1147
MaeIII GTNAC 4 cut(s) 179, 323, 420, 959
MalI GATC 8 cut(s) 824, 947, 1065, 1094, 1113, 1155, 1191, 1281
MboI GATC 8 cut(s) 822, 945, 1063, 1092, 1111, 1153, 1189, 1279
MboII GAAGA 5 cut(s) 27, 317, 905, 1085, 1148
MflI RGATCY 2 cut(s) 822, 1092
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 9 cut(s) 23, 156, 199, 480, 595, 852, 896, 1028, 1226
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 2 cut(s) 130, 351
MnlI CCTC 5 cut(s) 43, 709, 1069, 1204, 1247
Mox20I TGGCCA 1 cut(s) 414
MscI TGGCCA 1 cut(s) 414
MseI TTAA 6 cut(s) 159, 198, 579, 690, 705, 1241
MslI CAYNNNNRTG 2 cut(s) 429, 707
Msp20I TGGCCA 1 cut(s) 414
MspA1I CMGCKG 1 cut(s) 1277
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 3 cut(s) 213, 723, 981
Mva1269I GAATGC 1 cut(s) 114
MvaI CCWGG 2 cut(s) 723, 981
MwoI GCNNNNNNNGC 3 cut(s) 111, 1115, 1253
NciI CCSGG 1 cut(s) 213
NcoI CCATGG 1 cut(s) 409
NdeI CATATG 1 cut(s) 1258
NdeII GATC 8 cut(s) 822, 945, 1063, 1092, 1111, 1153, 1189, 1279
NlaIII CATG 9 cut(s) 31, 349, 413, 428, 512, 533, 892, 1010, 1171
NlaIV GGNNCC 2 cut(s) 720, 1173
NmuCI GTSAC 2 cut(s) 420, 959
NspI RCATGY 1 cut(s) 428
PceI AGGCCT 1 cut(s) 666
PcsI WCGNNNNNNNCGW 1 cut(s) 847
PctI GAATGC 1 cut(s) 114
PfeI GAWTC 4 cut(s) 137, 993, 1045, 1083
PfoI TCCNGGA 1 cut(s) 979
PkrI GCNGC 3 cut(s) 51, 241, 285
PleI GAGTC 2 cut(s) 129, 350
PmaCI CACGTG 1 cut(s) 863
PmlI CACGTG 1 cut(s) 863
PpsI GAGTC 2 cut(s) 129, 350
Ppu21I YACGTR 1 cut(s) 863
PsiI TTATAA 1 cut(s) 479
Psp1406I AACGTT 2 cut(s) 752, 1002
Psp6I CCWGG 2 cut(s) 721, 979
PspCI CACGTG 1 cut(s) 863
PspGI CCWGG 2 cut(s) 721, 979
PspN4I GGNNCC 2 cut(s) 720, 1173
PsuI RGATCY 2 cut(s) 822, 1092
PvuII CAGCTG 1 cut(s) 1277
RsaI GTAC 5 cut(s) 267, 383, 782, 789, 1173
RsaNI GTAC 5 cut(s) 266, 382, 781, 788, 1172
RseI CAYNNNNRTG 2 cut(s) 429, 707
SaqAI TTAA 6 cut(s) 159, 198, 579, 690, 705, 1241
SatI GCNGC 3 cut(s) 50, 240, 284
Sau3AI GATC 8 cut(s) 822, 945, 1063, 1092, 1111, 1153, 1189, 1279
ScaI AGTACT 1 cut(s) 267
SchI GAGTC 2 cut(s) 130, 351
ScrFI CCNGG 3 cut(s) 213, 723, 981
SfaNI GCATC 1 cut(s) 226
SfcI CTRYAG 1 cut(s) 190
SmiMI CAYNNNNRTG 2 cut(s) 429, 707
SmlI CTYRAG 2 cut(s) 109, 1123
SmoI CTYRAG 2 cut(s) 109, 1123
Sse9I AATT 9 cut(s) 23, 156, 199, 480, 595, 852, 896, 1028, 1226
SseBI AGGCCT 1 cut(s) 666
SsiI CCGC 5 cut(s) 145, 273, 284, 515, 1262
SspI AATATT 1 cut(s) 976
SspMI CTAG 2 cut(s) 287, 1319
StuI AGGCCT 1 cut(s) 666
StyD4I CCNGG 3 cut(s) 211, 721, 979
StyI CCWWGG 2 cut(s) 409, 661
TaaI ACNGT 4 cut(s) 179, 316, 323, 1058
TaiI ACGT 5 cut(s) 755, 844, 865, 1005, 1150
TaqI TCGA 1 cut(s) 354
TasI AATT 9 cut(s) 23, 156, 199, 480, 595, 852, 896, 1028, 1226
TatI WGTACW 1 cut(s) 265
TauI GCSGC 1 cut(s) 286
TfiI GAWTC 4 cut(s) 137, 993, 1045, 1083
Tru1I TTAA 6 cut(s) 159, 198, 579, 690, 705, 1241
Tru9I TTAA 6 cut(s) 159, 198, 579, 690, 705, 1241
TscAI CASTG 2 cut(s) 321, 1061
TseFI GTSAC 2 cut(s) 420, 959
TseI GCWGC 2 cut(s) 49, 239
Tsp45I GTSAC 2 cut(s) 420, 959
TspDTI ATGAA 9 cut(s) 16, 17, 36, 50, 531, 552, 825, 870, 995
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 2 cut(s) 321, 1061
XapI RAATTY 5 cut(s) 23, 595, 896, 1028, 1226
XceI RCATGY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 1189
XspI CTAG 2 cut(s) 287, 1319
ZrmI AGTACT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.