MD17G1177600.v1.1

DNA polymerase I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004379
Physical Location & Seq
Reverse (-)
20298413 .. 20300810
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1177600.v1.1.491

Sequence Viewer

Length: 306 bp
ATGCTGCTGGCTTCGACTTTGATAAAGCATGAAGAGAAAACAGTGAATAGTAACCCTTCAGATGGTAGAGTAATGCTCGTCGACGGCACATCCATCATTTATAGAGCATACTATAAGATTTTTGTAGCAAAGCTGCATCATGGACATCTGTCACATGCCGTTGGAAACGGAGATTGGGTGTTGACCATCTTCTCAGCACTTTCTCTTATAATTGATGTCCTGATGTTTATCCCTTCCCATGTGGCAGTGGTGTTTGACCGTGATGGTGTGATACAAATTTTGTTGGCCTCTTCATTTTGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_3_exonuc 5_3_exonuc_N

Protein Analysis

102

Amino Acids

11.2

Weight (kDa)

6.56

Isoelectric Point (pI)

10.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_3_exonuc_N PF02739 24 - 88 8.9e-07 5'-3' exonuclease, N-terminal resolvase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 209
AccI GTMKAC 1 cut(s) 81
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 62
AjuI GAANNNNNNNTTGG 2 cut(s) 157, 189
AluBI AGCT 1 cut(s) 133
AluI AGCT 1 cut(s) 133
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 2 cut(s) 4, 133
ApoI RAATTY 1 cut(s) 276
BbvI GCAGC 1 cut(s) 120
BccI CCATC 4 cut(s) 56, 101, 194, 257
BceAI ACGGC 2 cut(s) 100, 143
BisI GCNGC 2 cut(s) 5, 134
BlsI GCNGC 2 cut(s) 6, 135
BmsI GCATC 1 cut(s) 145
BoxI GACNNNNGTC 1 cut(s) 148
BplI GAGNNNNNCTC 2 cut(s) 60, 92
BsaBI GATNNNNATC 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse8I GATNNNNATC 1 cut(s) 227
BseGI GGATG 1 cut(s) 89
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 1 cut(s) 207
BseXI GCAGC 1 cut(s) 120
BshFI GGCC 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 62
BsnI GGCC 1 cut(s) 287
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 1 cut(s) 206
Bst4CI ACNGT 2 cut(s) 43, 260
Bst6I CTCTTC 2 cut(s) 27, 295
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 1 cut(s) 193
BstF5I GGATG 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 158
BstPAI GACNNNNGTC 1 cut(s) 148
BstV1I GCAGC 1 cut(s) 120
BsuRI GGCC 1 cut(s) 287
BtsCI GGATG 1 cut(s) 89
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 48, 252
Cac8I GCNNGC 1 cut(s) 9
CviAII CATG 4 cut(s) 29, 140, 155, 239
CviJI RGCY 3 cut(s) 11, 133, 287
CviKI_1 RGCY 3 cut(s) 11, 133, 287
DdeI CTNAG 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 27, 295
EarI CTCTTC 2 cut(s) 27, 295
Eco57I CTGAAG 1 cut(s) 42
FaeI CATG 4 cut(s) 32, 143, 158, 242
FaiI YATR 8 cut(s) 30, 102, 109, 114, 141, 156, 209, 240
FatI CATG 4 cut(s) 28, 139, 154, 238
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 2 cut(s) 5, 134
FokI GGATG 1 cut(s) 76
Fsp4HI GCNGC 2 cut(s) 5, 134
GluI GCNGC 2 cut(s) 5, 134
HaeIII GGCC 1 cut(s) 287
Hin1II CATG 4 cut(s) 32, 143, 158, 242
HincII GTYRAC 2 cut(s) 82, 183
HindII GTYRAC 2 cut(s) 82, 183
Hpy166II GTNNAC 2 cut(s) 82, 183
Hpy188I TCNGA 1 cut(s) 61
Hpy188III TCNNGA 1 cut(s) 220
Hpy8I GTNNAC 2 cut(s) 82, 183
Hpy99I CGWCG 2 cut(s) 83, 86
HpyAV CCTTC 2 cut(s) 66, 243
HpyCH4III ACNGT 2 cut(s) 43, 260
HpyCH4V TGCA 1 cut(s) 136
HpyF3I CTNAG 1 cut(s) 193
Hsp92II CATG 4 cut(s) 32, 143, 158, 242
LpnPI CCDG 1 cut(s) 233
Lsp1109I GCAGC 1 cut(s) 120
LweI GCATC 1 cut(s) 145
MaeIII GTNAC 2 cut(s) 50, 150
MboII GAAGA 3 cut(s) 44, 181, 282
MluCI AATT 2 cut(s) 210, 276
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 1 cut(s) 298
NlaIII CATG 4 cut(s) 32, 143, 158, 242
NmuCI GTSAC 1 cut(s) 150
NspI RCATGY 1 cut(s) 158
PkrI GCNGC 2 cut(s) 6, 135
PshAI GACNNNNGTC 1 cut(s) 148
PsiI TTATAA 1 cut(s) 209
SalI GTCGAC 1 cut(s) 80
SatI GCNGC 2 cut(s) 5, 134
SetI ASST 1 cut(s) 135
SfaNI GCATC 1 cut(s) 145
SgeI CNNG 8 cut(s) 20, 41, 89, 152, 167, 232, 251, 272
SgrDI CGTCGACG 1 cut(s) 80
Sse9I AATT 2 cut(s) 210, 276
TaaI ACNGT 2 cut(s) 43, 260
TaqI TCGA 2 cut(s) 14, 81
TasI AATT 2 cut(s) 210, 276
TscAI CASTG 2 cut(s) 48, 252
TseFI GTSAC 1 cut(s) 150
TseI GCWGC 2 cut(s) 4, 133
Tsp45I GTSAC 1 cut(s) 150
TspDTI ATGAA 2 cut(s) 45, 282
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 2 cut(s) 48, 252
XapI RAATTY 1 cut(s) 276
XceI RCATGY 1 cut(s) 158
XmiI GTMKAC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.