Rh4AG135400

DNA polymerase I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004379
Physical Location & Seq
Forward (+)
29759043 .. 29771291
12249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG135400.1

Sequence Viewer

Length: 1476 bp
ATGCCCGTTTTCAACCAAAAAAAAAAAATCTTATTTGGTGGCAAGTTAAAGCGTGCGGCGTCGTGTAAATTTCATTTGGGCGGGGGGATAGAGCTCGTGGTGTTGTTGGACATTGGAGAATGGAGAGCGAGCGAGTGTGAGATGGTTCATATCTTATTTATGGCCTGCTCTTTCTCTTCGCTGCTTCACTTTCAATCCCTTTGGAGAAGCTTCAAGTTGTTTGGCCCAGGAGCTCACAAGAGGCTCGGGCACCACTTGCGCACTCCCAACAAGCTCACTTGTCTCCATTTTTCTCCTATATCATCCGCAAAGGTATTATCTATACCCCCACAAATCAACAATTCATTATCTCAGGGACATTGTAATCTAGCCACCAGTGTTCACTCGGCATTACCTGGACTTGTTCATGACAATGGAGATTTGCTGGTTGATTCGGGGAAGCGTGAAGAGAAAATTGTATATAGTAACCCTTCAAATGGTAGAGTAATGCTCATTGACGGCACATCCATCATCTATAGATCATACTACAAGCTTCTTGCCAAGTTACACCATGGCCATCTGACACATGCTGATGGAAATGGAGATTGGGTGCTGACCATATTTACAGCGCTTTCTCTTATAATTGATGTGCTACAGTTTGTTCCTTCCCATGTGGCGGTGGTGTTTGACCATGATGGAGGTTCATTTGGTCAAACTAGTGTTTCATCCAAAGAAAGTGTTAGAGGAAAAGGCATGAATTTTCGTCATACTTTATACCCCGCATACAAGAGCAATCGCCCCCCTACACCAGATACCATTGTTCAGGGACTTCAGTACTTGAAAGCATCCCTCAAATCTATGTCCATCACGGTGATTGAGGTTCCAGGTGTGGAAGCTGATGATGTGATTGGAACATTGGCTGTTAGGAGTGTTGATAATGGTTACAAGGTACGAGTTGTCTCCCCAGACAAAGACTTTTTTCAGATCCTATCTCCTTCATTGCGTCTTCTACGAATTGCTTCACGTGGCTTTGAGATGGTTTCGTTTGGAATGGAGGACTTTGCTAAAAAATATGGAACCTTGCAACCCTCTCAGTTTGTTGATGTGATGTCACTTGTTGGTGACAAATGTGATAACATTCCAGGGGTTGATGGAATTGGAAACGTTCATGCTCTGCAACTCATCACTAAATTTGCCTCATTGGAGAATTTATTGCAGTGTGTGCATCAAGTTGAGGAAGAACGAATCAGAAAGGCCTTGATAGCAGGTGCTGAACAGGCTATATTAAGCAAGAACTTGGCACTATTACGTTCTGATCTTCCATTCTACATGGTACCATTCAATACAAAAGATCTGGCATTTATAAAACCTGAGGATGATGGAGAGAAATTCAGGAGTCTCTTGACTGCCATTAGTGCGTATGCGGAAGGATTTTCTGCTGATCCAGTCATAAGAAGAGCATTTTACTTATGGAACAAACTACAAAACCATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_3_exonuc 5_3_exonuc_N

Protein Analysis

491

Amino Acids

54.36

Weight (kDa)

8.97

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_3_exonuc_N PF02739 162 - 229 3.1e-07 5'-3' exonuclease, N-terminal resolvase-like domain
5_3_exonuc_N PF02739 244 - 352 2.1e-28 5'-3' exonuclease, N-terminal resolvase-like domain
5_3_exonuc PF01367 355 - 450 3e-24 5'-3' exonuclease, C-terminal SAM fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 620, 1343
AarI CACCTGC 1 cut(s) 1235
Acc16I TGCGCA 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 1235
Acc65I GGTACC 1 cut(s) 1312
AccB1I GGYRCC 2 cut(s) 249, 1312
AciI CCGC 6 cut(s) 56, 81, 306, 656, 759, 1403
AclI AACGTT 1 cut(s) 1143
AclWI GGATC 2 cut(s) 958, 1415
AcoI YGGCCR 1 cut(s) 553
AcsI RAATTY 5 cut(s) 68, 736, 1169, 1186, 1367
AcuI CTGAAG 1 cut(s) 794
AcvI CACGTG 1 cut(s) 1004
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 3 cut(s) 815, 930, 1314
AfeI AGCGCT 1 cut(s) 609
AfiI CCNNNNNNNGG 2 cut(s) 476, 655
AgsI TTSAA 6 cut(s) 13, 194, 214, 474, 820, 1321
AhlI ACTAGT 1 cut(s) 695
AjnI CCWGG 4 cut(s) 226, 394, 862, 1120
AjuI GAANNNNNNNTTGG 2 cut(s) 568, 600
AluBI AGCT 6 cut(s) 94, 210, 233, 274, 532, 875
AluI AGCT 6 cut(s) 94, 210, 233, 274, 532, 875
Alw21I GWGCWC 2 cut(s) 96, 235
Alw26I GTCTC 3 cut(s) 287, 943, 1382
AlwI GGATC 2 cut(s) 958, 1415
AlwNI CAGNNNCTG 1 cut(s) 1250
Ama87I CYCGRG 1 cut(s) 245
Aor51HI AGCGCT 1 cut(s) 609
AoxI GGCC 4 cut(s) 162, 223, 553, 1233
ApeKI GCWGC 1 cut(s) 181
ApoI RAATTY 5 cut(s) 68, 736, 1169, 1186, 1367
Asp700I GAANNNNTTC 1 cut(s) 997
Asp718I GGTACC 1 cut(s) 1312
AspLEI GCGC 2 cut(s) 261, 610
AspS9I GGNCC 1 cut(s) 224
AsuHPI GGTGA 2 cut(s) 862, 1112
AvaI CYCGRG 1 cut(s) 245
AxyI CCTNAGG 1 cut(s) 1350
BaeGI GKGCMC 1 cut(s) 252
BaeI ACNNNNGTAYC 2 cut(s) 783, 816
BalI TGGCCA 1 cut(s) 555
BanI GGYRCC 2 cut(s) 249, 1312
BanII GRGCYC 2 cut(s) 96, 235
BauI CACGAG 1 cut(s) 95
BbrPI CACGTG 1 cut(s) 1004
BbsI GAAGAC 1 cut(s) 977
Bbv12I GWGCWC 2 cut(s) 96, 235
BbvI GCAGC 1 cut(s) 168
BccI CCATC 9 cut(s) 136, 515, 564, 566, 668, 851, 1009, 1124, 1352
BceAI ACGGC 1 cut(s) 514
BciT130I CCWGG 4 cut(s) 228, 396, 864, 1122
BcoDI GTCTC 3 cut(s) 287, 943, 1382
BcuI ACTAGT 1 cut(s) 695
BfaI CTAG 2 cut(s) 368, 696
BfmI CTRYAG 2 cut(s) 514, 632
BfoI RGCGCY 1 cut(s) 611
BfuAI ACCTGC 1 cut(s) 1235
BglII AGATCT 1 cut(s) 1330
BisI GCNGC 2 cut(s) 57, 182
BlsI GCNGC 2 cut(s) 58, 183
BmcAI AGTACT 1 cut(s) 815
Bme1390I CCNGG 4 cut(s) 228, 396, 864, 1122
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 4 cut(s) 251, 861, 1057, 1314
BmrFI CCNGG 4 cut(s) 228, 396, 864, 1122
BmsI GCATC 2 cut(s) 833, 1213
BpiI GAAGAC 1 cut(s) 977
BplI GAGNNNNNCTC 2 cut(s) 474, 506
BsaAI YACGTR 1 cut(s) 1004
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 226, 550, 1121
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 655
Bse1I ACTGG 2 cut(s) 375, 1424
Bse21I CCTNAGG 1 cut(s) 1350
Bse3DI GCAATG 1 cut(s) 977
BseBI CCWGG 4 cut(s) 228, 396, 864, 1122
BseDI CCNNGG 3 cut(s) 226, 550, 1121
BseGI GGATG 5 cut(s) 302, 503, 704, 824, 1360
BseLI CCNNNNNNNGG 2 cut(s) 476, 655
BseMI GCAATG 1 cut(s) 977
BseMII CTCAG 3 cut(s) 365, 1085, 1341
BseNI ACTGG 2 cut(s) 375, 1424
BseSI GKGCMC 1 cut(s) 252
BseXI GCAGC 1 cut(s) 168
BshFI GGCC 4 cut(s) 164, 225, 555, 1235
BshNI GGYRCC 2 cut(s) 249, 1312
BsiHKAI GWGCWC 2 cut(s) 96, 235
BsiHKCI CYCGRG 1 cut(s) 245
BslFI GGGAC 2 cut(s) 369, 819
BslI CCNNNNNNNGG 2 cut(s) 476, 655
BsmAI GTCTC 3 cut(s) 287, 943, 1382
BsmFI GGGAC 2 cut(s) 369, 819
BsnI GGCC 4 cut(s) 164, 225, 555, 1235
BsoBI CYCGRG 1 cut(s) 245
Bsp1286I GDGCHC 3 cut(s) 96, 235, 252
Bsp143I GATC 5 cut(s) 518, 963, 1294, 1330, 1420
Bsp19I CCATGG 1 cut(s) 550
BspACI CCGC 6 cut(s) 56, 81, 306, 656, 759, 1403
BspANI GGCC 4 cut(s) 164, 225, 555, 1235
BspCNI CTCAG 3 cut(s) 364, 1084, 1342
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 4 cut(s) 251, 861, 1057, 1314
BspMI ACCTGC 1 cut(s) 1235
BspPI GGATC 2 cut(s) 958, 1415
BspQI GCTCTTC 1 cut(s) 1429
BspT107I GGYRCC 2 cut(s) 249, 1312
BsrDI GCAATG 1 cut(s) 977
BsrI ACTGG 2 cut(s) 375, 1424
BssECI CCNNGG 3 cut(s) 226, 550, 1121
BssMI GATC 5 cut(s) 518, 963, 1294, 1330, 1420
BssNI GRCGYC 1 cut(s) 59
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 550
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 4 cut(s) 228, 396, 864, 1122
Bst4CI ACNGT 2 cut(s) 636, 850
Bst6I CTCTTC 3 cut(s) 181, 441, 1429
BstACI GRCGYC 1 cut(s) 59
BstAPI GCANNNNNTGC 2 cut(s) 256, 1201
BstBAI YACGTR 1 cut(s) 1004
BstC8I GCNNGC 3 cut(s) 54, 130, 166
BstDEI CTNAG 3 cut(s) 351, 1071, 1350
BstDSI CCRYGG 1 cut(s) 550
BstF5I GGATG 5 cut(s) 302, 503, 704, 824, 1360
BstH2I RGCGCY 1 cut(s) 611
BstHHI GCGC 2 cut(s) 261, 610
BstKTI GATC 5 cut(s) 521, 966, 1297, 1333, 1423
BstMAI GTCTC 3 cut(s) 287, 943, 1382
BstMBI GATC 5 cut(s) 518, 963, 1294, 1330, 1420
BstMWI GCNNNNNNNGC 5 cut(s) 256, 1201, 1241, 1256, 1394
BstNI CCWGG 4 cut(s) 228, 396, 864, 1122
BstNSI RCATGY 1 cut(s) 569
BstSCI CCNGG 4 cut(s) 226, 394, 862, 1120
BstSFI CTRYAG 2 cut(s) 514, 632
BstSLI GKGCMC 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 168
BstV2I GAAGAC 1 cut(s) 977
BstX2I RGATCY 2 cut(s) 963, 1330
BstYI RGATCY 2 cut(s) 963, 1330
Bsu36I CCTNAGG 1 cut(s) 1350
BsuRI GGCC 4 cut(s) 164, 225, 555, 1235
BtgI CCRYGG 1 cut(s) 550
BtsCI GGATG 5 cut(s) 302, 503, 704, 824, 1360
BtsI GCAGTG 1 cut(s) 1202
BtsIMutI CAGTG 2 cut(s) 382, 1202
BveI ACCTGC 1 cut(s) 1235
Cac8I GCNNGC 3 cut(s) 54, 130, 166
CaiI CAGNNNCTG 1 cut(s) 1250
CciI TCATGA 1 cut(s) 406
CfoI GCGC 2 cut(s) 261, 610
Cfr13I GGNCC 1 cut(s) 224
CseI GACGC 2 cut(s) 48, 971
Csp6I GTAC 3 cut(s) 814, 929, 1313
CviAII CATG 8 cut(s) 407, 551, 566, 650, 671, 733, 1148, 1309
CviQI GTAC 3 cut(s) 814, 929, 1313
DdeI CTNAG 3 cut(s) 351, 1071, 1350
DpnI GATC 5 cut(s) 520, 965, 1296, 1332, 1422
DpnII GATC 5 cut(s) 518, 963, 1294, 1330, 1420
EaeI YGGCCR 1 cut(s) 553
Eam1104I CTCTTC 3 cut(s) 181, 441, 1429
EarI CTCTTC 3 cut(s) 181, 441, 1429
Ecl136II GAGCTC 2 cut(s) 94, 233
Eco130I CCWWGG 1 cut(s) 550
Eco147I AGGCCT 1 cut(s) 1235
Eco24I GRGCYC 2 cut(s) 96, 235
Eco47III AGCGCT 1 cut(s) 609
Eco53kI GAGCTC 2 cut(s) 94, 233
Eco57I CTGAAG 1 cut(s) 794
Eco72I CACGTG 1 cut(s) 1004
Eco81I CCTNAGG 1 cut(s) 1350
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 2 cut(s) 94, 233
EcoRII CCWGG 4 cut(s) 226, 394, 862, 1120
EcoT14I CCWWGG 1 cut(s) 550
EcoT38I GRGCYC 2 cut(s) 96, 235
ErhI CCWWGG 1 cut(s) 550
FaeI CATG 8 cut(s) 410, 554, 569, 653, 674, 736, 1151, 1312
FaqI GGGAC 2 cut(s) 369, 819
FatI CATG 8 cut(s) 406, 550, 565, 649, 670, 732, 1147, 1308
FauI CCCGC 2 cut(s) 74, 766
Fnu4HI GCNGC 2 cut(s) 57, 182
FokI GGATG 5 cut(s) 289, 490, 691, 811, 1367
FriOI GRGCYC 2 cut(s) 96, 235
Fsp4HI GCNGC 2 cut(s) 57, 182
FspBI CTAG 2 cut(s) 368, 696
FspI TGCGCA 1 cut(s) 260
GlaI GCGC 2 cut(s) 260, 609
GluI GCNGC 2 cut(s) 57, 182
HaeII RGCGCY 1 cut(s) 611
HaeIII GGCC 4 cut(s) 164, 225, 555, 1235
HgaI GACGC 2 cut(s) 48, 971
HhaI GCGC 2 cut(s) 261, 610
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 8 cut(s) 410, 554, 569, 653, 674, 736, 1151, 1312
Hin6I GCGC 2 cut(s) 259, 608
HinP1I GCGC 2 cut(s) 259, 608
HindIII AAGCTT 2 cut(s) 208, 530
HinfI GANTC 3 cut(s) 431, 1224, 1375
HphI GGTGA 2 cut(s) 862, 1112
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 4 cut(s) 561, 963, 1229, 1294
Hpy188III TCNNGA 3 cut(s) 407, 1372, 1381
Hpy8I GTNNAC 1 cut(s) 382
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 4 cut(s) 480, 654, 984, 1400
HpyCH4III ACNGT 2 cut(s) 636, 850
HpyCH4IV ACGT 3 cut(s) 1003, 1143, 1288
HpyCH4V TGCA 4 cut(s) 1063, 1156, 1195, 1204
HpyF10VI GCNNNNNNNGC 5 cut(s) 256, 1201, 1241, 1256, 1394
HpyF3I CTNAG 3 cut(s) 351, 1071, 1350
HpySE526I ACGT 3 cut(s) 1003, 1143, 1288
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 8 cut(s) 410, 554, 569, 653, 674, 736, 1151, 1312
HspAI GCGC 2 cut(s) 259, 608
KpnI GGTACC 1 cut(s) 1316
Kzo9I GATC 5 cut(s) 518, 963, 1294, 1330, 1420
LguI GCTCTTC 1 cut(s) 1429
LmnI GCTCC 1 cut(s) 230
Lsp1109I GCAGC 1 cut(s) 168
LweI GCATC 2 cut(s) 833, 1213
MaeI CTAG 2 cut(s) 368, 696
MaeII ACGT 3 cut(s) 1003, 1143, 1288
MaeIII GTNAC 5 cut(s) 464, 543, 920, 1089, 1100
MalI GATC 5 cut(s) 520, 965, 1296, 1332, 1422
MboI GATC 5 cut(s) 518, 963, 1294, 1330, 1420
MboII GAAGA 6 cut(s) 168, 458, 977, 1229, 1289, 1446
MflI RGATCY 2 cut(s) 963, 1330
MhlI GDGCHC 3 cut(s) 96, 235, 252
MlsI TGGCCA 1 cut(s) 555
MluNI TGGCCA 1 cut(s) 555
MlyI GAGTC 1 cut(s) 1384
MmeI TCCRAC 1 cut(s) 87
Mox20I TGGCCA 1 cut(s) 555
MroXI GAANNNNTTC 1 cut(s) 997
MscI TGGCCA 1 cut(s) 555
MseI TTAA 3 cut(s) 47, 1265, 1474
MslI CAYNNNNRTG 3 cut(s) 411, 570, 848
Msp20I TGGCCA 1 cut(s) 555
MspR9I CCNGG 4 cut(s) 228, 396, 864, 1122
MvaI CCWGG 4 cut(s) 228, 396, 864, 1122
MwoI GCNNNNNNNGC 5 cut(s) 256, 1201, 1241, 1256, 1394
NcoI CCATGG 1 cut(s) 550
NdeII GATC 5 cut(s) 518, 963, 1294, 1330, 1420
NlaIII CATG 8 cut(s) 410, 554, 569, 653, 674, 736, 1151, 1312
NlaIV GGNNCC 4 cut(s) 251, 861, 1057, 1314
NmeAIII GCCGAG 1 cut(s) 365
NmuCI GTSAC 2 cut(s) 1089, 1100
NsbI TGCGCA 1 cut(s) 260
NspI RCATGY 1 cut(s) 569
PagI TCATGA 1 cut(s) 406
PaqCI CACCTGC 1 cut(s) 1235
PceI AGGCCT 1 cut(s) 1235
PciSI GCTCTTC 1 cut(s) 1429
PdmI GAANNNNTTC 1 cut(s) 997
PfeI GAWTC 2 cut(s) 431, 1224
PkrI GCNGC 2 cut(s) 58, 183
PleI GAGTC 1 cut(s) 1383
PmaCI CACGTG 1 cut(s) 1004
PmlI CACGTG 1 cut(s) 1004
PpsI GAGTC 1 cut(s) 1383
Ppu21I YACGTR 1 cut(s) 1004
PsiI TTATAA 2 cut(s) 620, 1343
Psp124BI GAGCTC 2 cut(s) 96, 235
Psp1406I AACGTT 1 cut(s) 1143
Psp6I CCWGG 4 cut(s) 226, 394, 862, 1120
PspCI CACGTG 1 cut(s) 1004
PspGI CCWGG 4 cut(s) 226, 394, 862, 1120
PspN4I GGNNCC 4 cut(s) 251, 861, 1057, 1314
PspPI GGNCC 1 cut(s) 224
PstNI CAGNNNCTG 1 cut(s) 1250
PsuI RGATCY 2 cut(s) 963, 1330
RsaI GTAC 3 cut(s) 815, 930, 1314
RsaNI GTAC 3 cut(s) 814, 929, 1313
RseI CAYNNNNRTG 3 cut(s) 411, 570, 848
SacI GAGCTC 2 cut(s) 96, 235
SapI GCTCTTC 1 cut(s) 1429
SaqAI TTAA 3 cut(s) 47, 1265, 1474
SatI GCNGC 2 cut(s) 57, 182
Sau3AI GATC 5 cut(s) 518, 963, 1294, 1330, 1420
Sau96I GGNCC 1 cut(s) 224
ScaI AGTACT 1 cut(s) 815
SchI GAGTC 1 cut(s) 1384
ScrFI CCNGG 4 cut(s) 228, 396, 864, 1122
SduI GDGCHC 3 cut(s) 96, 235, 252
SfaNI GCATC 2 cut(s) 833, 1213
SfcI CTRYAG 2 cut(s) 514, 632
SmiMI CAYNNNNRTG 3 cut(s) 411, 570, 848
SpeI ACTAGT 1 cut(s) 695
SseBI AGGCCT 1 cut(s) 1235
SsiI CCGC 6 cut(s) 56, 81, 306, 656, 759, 1403
SspMI CTAG 2 cut(s) 368, 696
SstI GAGCTC 2 cut(s) 96, 235
StuI AGGCCT 1 cut(s) 1235
StyD4I CCNGG 4 cut(s) 226, 394, 862, 1120
StyI CCWWGG 1 cut(s) 550
TaaI ACNGT 2 cut(s) 636, 850
TaiI ACGT 3 cut(s) 1006, 1146, 1291
TatI WGTACW 1 cut(s) 813
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 2 cut(s) 431, 1224
Tru1I TTAA 3 cut(s) 47, 1265, 1474
Tru9I TTAA 3 cut(s) 47, 1265, 1474
TscAI CASTG 2 cut(s) 382, 1202
TseFI GTSAC 2 cut(s) 1089, 1100
TseI GCWGC 1 cut(s) 181
Tsp45I GTSAC 2 cut(s) 1089, 1100
TspDTI ATGAA 9 cut(s) 62, 137, 333, 395, 672, 693, 749, 966, 1136
TspRI CASTG 2 cut(s) 382, 1202
XapI RAATTY 5 cut(s) 68, 736, 1169, 1186, 1367
XceI RCATGY 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 997
XspI CTAG 2 cut(s) 368, 696
ZrmI AGTACT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.