Rw4G011020

DNA polymerase I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004379
Physical Location & Seq
Forward (+)
26147011 .. 26159147
12137 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011020.1

Sequence Viewer

Length: 1284 bp
ATGGCCTGCTCTTTCTCTTCGCTGCTTCACTTTCAATCCCTCTGGAGAAGCTTCAAGTTGTTTGGCCCAGGAGCTCACAAGAGGCTCGGGCACCACTTGCGCACTCCCAACAACCTCACTTGTCTCCATTTTTCTCCTATAACATCCGCAAAGGGACATTGTAATCTAGCCACCAGTGTTCACTCGGCATTACCCGGACTTGTTCATGACAATGGAGATTTGCTGGTTGATTCAGGGAAGCATGAACAGAAAGTTGTATATAGTAACCCTTCAAATGGTAGAGTAATGCTCATTGACGGCACATCCATCATCTATAGATCATACTACAAGCTTCTCGCCAAGTTACACCATGGCCATCTGACACATGCTGATGGAAATGGAGATTGGGTGCTGACCATATTTACAGCGCTTTCTCTTATAATTGATGTGCTACAGTTTGTTCCTTCCCATGTGGCGGTGGTGTTTGACCATGATGGAGGTTCATTTGGTCAAACTAGTGTTTCATCCAAAGAAAGTGTTAGAGGAAAAGGCATGAATTTTCGTCATACTTTATACCCCGCATACAAGAGCAATCGCCCCCCTACACCAGATACCATTGTTCAGGGACTTCAGTACTTGAAAGCATCCCTCAAATCTATGTCCATCACGGTGATTGAGGTTCCAGGTGTGGAAGCTGATGATGTGATTGGAACATTGGCTGTTAGGAGTGTTGATAATGGTTACAAGGTACGAGTTGTCTCCCCAGACAAAGACTTTTTTCAGATCCTATCTCCTTCATTGCGTCTTCTACGAATTGCTTCACGTGGCTTTGAGATGGTTTCGTTTGGAATGGAGGACTTTGCTAAAAAATATGGAACCTTGCAACCCTCTCAGTTTGTTGATGTGATGTCACTTGTTGGTGACAAATGTGATAACATTCCAGGGGTTGATGGAATTGGAAACGTTCATGCTCTGCAACTCATCACTAAATTTGCCTCATTGGAGAATTTATTGCAGTGTGTGCATCAAGTTGAGGAAGAACGAATCAGAAAGGCCTTGATAGCAGGTGCTGAACAGGCTATATTAAGCAAGAACTTGGCACTATTACGTTCTGATCTTCCATTCTACATGGTACCATTCAATACAAAAGATCTGGCATTTATAAAACCTGAGGATGATGGAGAGAAATTCAGGAGTCTCCTCACTGCCATTAGTGCGTATGCAGAAGGATTTTCTGCTGATCCAGTCATAAGAAGAGCATTTTACTTATGGAACAAACTACAAAACCATAACCAAGTTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_3_exonuc 5_3_exonuc_N

Protein Analysis

427

Amino Acids

47.21

Weight (kDa)

8.65

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_3_exonuc_N PF02739 95 - 162 2.5e-07 5'-3' exonuclease, N-terminal resolvase-like domain
5_3_exonuc_N PF02739 177 - 285 1.7e-28 5'-3' exonuclease, N-terminal resolvase-like domain
5_3_exonuc PF01367 288 - 383 2.4e-24 5'-3' exonuclease, C-terminal SAM fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 419, 1142
AarI CACCTGC 1 cut(s) 1034
Acc16I TGCGCA 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 1034
Acc65I GGTACC 1 cut(s) 1111
AccB1I GGYRCC 2 cut(s) 90, 1111
AciI CCGC 3 cut(s) 147, 455, 558
AclI AACGTT 1 cut(s) 942
AclWI GGATC 2 cut(s) 757, 1214
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 4 cut(s) 535, 968, 985, 1166
AcuI CTGAAG 1 cut(s) 593
AcvI CACGTG 1 cut(s) 803
AfaI GTAC 3 cut(s) 614, 729, 1113
AfeI AGCGCT 1 cut(s) 408
AfiI CCNNNNNNNGG 2 cut(s) 275, 454
AgsI TTSAA 5 cut(s) 35, 55, 273, 619, 1120
AhlI ACTAGT 1 cut(s) 494
AjnI CCWGG 3 cut(s) 67, 661, 919
AjuI GAANNNNNNNTTGG 2 cut(s) 367, 399
AluBI AGCT 4 cut(s) 51, 74, 331, 674
AluI AGCT 4 cut(s) 51, 74, 331, 674
Alw21I GWGCWC 1 cut(s) 76
Alw26I GTCTC 3 cut(s) 128, 742, 1181
AlwI GGATC 2 cut(s) 757, 1214
AlwNI CAGNNNCTG 1 cut(s) 1049
Ama87I CYCGRG 1 cut(s) 86
Aor51HI AGCGCT 1 cut(s) 408
AoxI GGCC 4 cut(s) 3, 64, 352, 1032
ApeKI GCWGC 1 cut(s) 22
ApoI RAATTY 4 cut(s) 535, 968, 985, 1166
Asp700I GAANNNNTTC 1 cut(s) 796
Asp718I GGTACC 1 cut(s) 1111
AspLEI GCGC 2 cut(s) 102, 409
AspS9I GGNCC 1 cut(s) 65
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 2 cut(s) 661, 911
AvaI CYCGRG 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 1149
BaeGI GKGCMC 1 cut(s) 93
BaeI ACNNNNGTAYC 2 cut(s) 582, 615
BalI TGGCCA 1 cut(s) 354
BanI GGYRCC 2 cut(s) 90, 1111
BanII GRGCYC 1 cut(s) 76
BbrPI CACGTG 1 cut(s) 803
BbsI GAAGAC 1 cut(s) 776
Bbv12I GWGCWC 1 cut(s) 76
BbvI GCAGC 1 cut(s) 9
BccI CCATC 8 cut(s) 314, 363, 365, 467, 650, 808, 923, 1151
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 3 cut(s) 69, 663, 921
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 3 cut(s) 128, 742, 1181
BcuI ACTAGT 1 cut(s) 494
BfaI CTAG 3 cut(s) 167, 495, 1282
BfmI CTRYAG 2 cut(s) 313, 431
BfoI RGCGCY 1 cut(s) 410
BfuAI ACCTGC 1 cut(s) 1034
BglII AGATCT 1 cut(s) 1129
BisI GCNGC 1 cut(s) 23
BlsI GCNGC 1 cut(s) 24
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 4 cut(s) 69, 195, 663, 921
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 65
BmiI GGNNCC 4 cut(s) 92, 660, 856, 1113
BmrFI CCNGG 4 cut(s) 69, 195, 663, 921
BmsI GCATC 2 cut(s) 632, 1012
BpiI GAAGAC 1 cut(s) 776
BplI GAGNNNNNCTC 2 cut(s) 273, 305
BpmI CTGGAG 1 cut(s) 64
BpuMI CCSGG 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 803
BsaJI CCNNGG 3 cut(s) 67, 349, 920
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 454
Bse1I ACTGG 2 cut(s) 174, 1223
Bse21I CCTNAGG 1 cut(s) 1149
Bse3DI GCAATG 1 cut(s) 776
BseBI CCWGG 3 cut(s) 69, 663, 921
BseDI CCNNGG 3 cut(s) 67, 349, 920
BseGI GGATG 5 cut(s) 143, 302, 503, 623, 1159
BseLI CCNNNNNNNGG 2 cut(s) 275, 454
BseMI GCAATG 1 cut(s) 776
BseMII CTCAG 2 cut(s) 884, 1140
BseNI ACTGG 2 cut(s) 174, 1223
BseRI GAGGAG 1 cut(s) 1169
BseSI GKGCMC 1 cut(s) 93
BseXI GCAGC 1 cut(s) 9
BshFI GGCC 4 cut(s) 5, 66, 354, 1034
BshNI GGYRCC 2 cut(s) 90, 1111
BsiHKAI GWGCWC 1 cut(s) 76
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 2 cut(s) 168, 618
BslI CCNNNNNNNGG 2 cut(s) 275, 454
BsmAI GTCTC 3 cut(s) 128, 742, 1181
BsmFI GGGAC 2 cut(s) 168, 618
BsnI GGCC 4 cut(s) 5, 66, 354, 1034
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 76, 93
Bsp143I GATC 5 cut(s) 317, 762, 1093, 1129, 1219
Bsp19I CCATGG 1 cut(s) 349
BspACI CCGC 3 cut(s) 147, 455, 558
BspANI GGCC 4 cut(s) 5, 66, 354, 1034
BspCNI CTCAG 2 cut(s) 883, 1141
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 4 cut(s) 92, 660, 856, 1113
BspMI ACCTGC 1 cut(s) 1034
BspPI GGATC 2 cut(s) 757, 1214
BspQI GCTCTTC 1 cut(s) 1228
BspT107I GGYRCC 2 cut(s) 90, 1111
BsrDI GCAATG 1 cut(s) 776
BsrI ACTGG 2 cut(s) 174, 1223
BssECI CCNNGG 3 cut(s) 67, 349, 920
BssMI GATC 5 cut(s) 317, 762, 1093, 1129, 1219
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 3 cut(s) 69, 663, 921
Bst4CI ACNGT 2 cut(s) 435, 649
Bst6I CTCTTC 2 cut(s) 22, 1228
BstAPI GCANNNNNTGC 2 cut(s) 97, 1000
BstBAI YACGTR 1 cut(s) 803
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 870, 1149
BstDSI CCRYGG 1 cut(s) 349
BstF5I GGATG 5 cut(s) 143, 302, 503, 623, 1159
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 2 cut(s) 102, 409
BstKTI GATC 5 cut(s) 320, 765, 1096, 1132, 1222
BstMAI GTCTC 3 cut(s) 128, 742, 1181
BstMBI GATC 5 cut(s) 317, 762, 1093, 1129, 1219
BstMWI GCNNNNNNNGC 5 cut(s) 97, 1000, 1040, 1055, 1193
BstNI CCWGG 3 cut(s) 69, 663, 921
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 4 cut(s) 67, 193, 661, 919
BstSFI CTRYAG 2 cut(s) 313, 431
BstSLI GKGCMC 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 9
BstV2I GAAGAC 1 cut(s) 776
BstX2I RGATCY 2 cut(s) 762, 1129
BstYI RGATCY 2 cut(s) 762, 1129
Bsu36I CCTNAGG 1 cut(s) 1149
BsuRI GGCC 4 cut(s) 5, 66, 354, 1034
BtgI CCRYGG 1 cut(s) 349
BtsCI GGATG 5 cut(s) 143, 302, 503, 623, 1159
BtsI GCAGTG 2 cut(s) 1001, 1182
BtsIMutI CAGTG 3 cut(s) 181, 1001, 1182
BveI ACCTGC 1 cut(s) 1034
Cac8I GCNNGC 1 cut(s) 7
CaiI CAGNNNCTG 1 cut(s) 1049
CciI TCATGA 1 cut(s) 205
CfoI GCGC 2 cut(s) 102, 409
Cfr13I GGNCC 1 cut(s) 65
CseI GACGC 1 cut(s) 770
Csp6I GTAC 3 cut(s) 613, 728, 1112
CviAII CATG 9 cut(s) 206, 242, 350, 365, 449, 470, 532, 947, 1108
CviQI GTAC 3 cut(s) 613, 728, 1112
DdeI CTNAG 2 cut(s) 870, 1149
DpnI GATC 5 cut(s) 319, 764, 1095, 1131, 1221
DpnII GATC 5 cut(s) 317, 762, 1093, 1129, 1219
EaeI YGGCCR 1 cut(s) 352
Eam1104I CTCTTC 2 cut(s) 22, 1228
EarI CTCTTC 2 cut(s) 22, 1228
Ecl136II GAGCTC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 349
Eco147I AGGCCT 1 cut(s) 1034
Eco24I GRGCYC 1 cut(s) 76
Eco47III AGCGCT 1 cut(s) 408
Eco53kI GAGCTC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 593
Eco72I CACGTG 1 cut(s) 803
Eco81I CCTNAGG 1 cut(s) 1149
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 74
EcoRII CCWGG 3 cut(s) 67, 661, 919
EcoT14I CCWWGG 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 76
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 9 cut(s) 209, 245, 353, 368, 452, 473, 535, 950, 1111
FaqI GGGAC 2 cut(s) 168, 618
FatI CATG 9 cut(s) 205, 241, 349, 364, 448, 469, 531, 946, 1107
FauI CCCGC 1 cut(s) 565
Fnu4HI GCNGC 1 cut(s) 23
FokI GGATG 5 cut(s) 130, 289, 490, 610, 1166
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 1 cut(s) 23
FspBI CTAG 3 cut(s) 167, 495, 1282
FspI TGCGCA 1 cut(s) 101
GlaI GCGC 2 cut(s) 101, 408
GluI GCNGC 1 cut(s) 23
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 1 cut(s) 410
HaeIII GGCC 4 cut(s) 5, 66, 354, 1034
HapII CCGG 1 cut(s) 195
HgaI GACGC 1 cut(s) 770
HhaI GCGC 2 cut(s) 102, 409
Hin1II CATG 9 cut(s) 209, 245, 353, 368, 452, 473, 535, 950, 1111
Hin6I GCGC 2 cut(s) 100, 407
HinP1I GCGC 2 cut(s) 100, 407
HindIII AAGCTT 2 cut(s) 49, 329
HinfI GANTC 3 cut(s) 230, 1023, 1174
HpaII CCGG 1 cut(s) 195
HphI GGTGA 2 cut(s) 661, 911
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 360, 762, 1028, 1093
Hpy188III TCNNGA 3 cut(s) 43, 206, 1171
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 4 cut(s) 279, 453, 783, 1199
HpyCH4III ACNGT 2 cut(s) 435, 649
HpyCH4IV ACGT 3 cut(s) 802, 942, 1087
HpyCH4V TGCA 5 cut(s) 862, 955, 994, 1003, 1202
HpyF10VI GCNNNNNNNGC 5 cut(s) 97, 1000, 1040, 1055, 1193
HpyF3I CTNAG 2 cut(s) 870, 1149
HpySE526I ACGT 3 cut(s) 802, 942, 1087
Hsp92II CATG 9 cut(s) 209, 245, 353, 368, 452, 473, 535, 950, 1111
HspAI GCGC 2 cut(s) 100, 407
KpnI GGTACC 1 cut(s) 1115
Kzo9I GATC 5 cut(s) 317, 762, 1093, 1129, 1219
LguI GCTCTTC 1 cut(s) 1228
LmnI GCTCC 1 cut(s) 71
Lsp1109I GCAGC 1 cut(s) 9
LweI GCATC 2 cut(s) 632, 1012
MaeI CTAG 3 cut(s) 167, 495, 1282
MaeII ACGT 3 cut(s) 802, 942, 1087
MaeIII GTNAC 5 cut(s) 263, 342, 719, 888, 899
MalI GATC 5 cut(s) 319, 764, 1095, 1131, 1221
MboI GATC 5 cut(s) 317, 762, 1093, 1129, 1219
MboII GAAGA 5 cut(s) 9, 776, 1028, 1088, 1245
MflI RGATCY 2 cut(s) 762, 1129
MhlI GDGCHC 2 cut(s) 76, 93
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 7 cut(s) 420, 535, 792, 933, 968, 985, 1166
MluNI TGGCCA 1 cut(s) 354
MlyI GAGTC 1 cut(s) 1183
Mox20I TGGCCA 1 cut(s) 354
MroXI GAANNNNTTC 1 cut(s) 796
MscI TGGCCA 1 cut(s) 354
MseI TTAA 1 cut(s) 1064
MslI CAYNNNNRTG 3 cut(s) 210, 369, 647
Msp20I TGGCCA 1 cut(s) 354
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 4 cut(s) 69, 195, 663, 921
MvaI CCWGG 3 cut(s) 69, 663, 921
MwoI GCNNNNNNNGC 5 cut(s) 97, 1000, 1040, 1055, 1193
NciI CCSGG 1 cut(s) 195
NcoI CCATGG 1 cut(s) 349
NdeII GATC 5 cut(s) 317, 762, 1093, 1129, 1219
NlaIII CATG 9 cut(s) 209, 245, 353, 368, 452, 473, 535, 950, 1111
NlaIV GGNNCC 4 cut(s) 92, 660, 856, 1113
NmeAIII GCCGAG 1 cut(s) 164
NmuCI GTSAC 2 cut(s) 888, 899
NsbI TGCGCA 1 cut(s) 101
NspI RCATGY 1 cut(s) 368
PagI TCATGA 1 cut(s) 205
PaqCI CACCTGC 1 cut(s) 1034
PceI AGGCCT 1 cut(s) 1034
PciSI GCTCTTC 1 cut(s) 1228
PdmI GAANNNNTTC 1 cut(s) 796
PfeI GAWTC 2 cut(s) 230, 1023
PkrI GCNGC 1 cut(s) 24
PleI GAGTC 1 cut(s) 1182
PmaCI CACGTG 1 cut(s) 803
PmlI CACGTG 1 cut(s) 803
PpsI GAGTC 1 cut(s) 1182
Ppu21I YACGTR 1 cut(s) 803
PsiI TTATAA 2 cut(s) 419, 1142
Psp124BI GAGCTC 1 cut(s) 76
Psp1406I AACGTT 1 cut(s) 942
Psp6I CCWGG 3 cut(s) 67, 661, 919
PspCI CACGTG 1 cut(s) 803
PspGI CCWGG 3 cut(s) 67, 661, 919
PspN4I GGNNCC 4 cut(s) 92, 660, 856, 1113
PspPI GGNCC 1 cut(s) 65
PstNI CAGNNNCTG 1 cut(s) 1049
PsuI RGATCY 2 cut(s) 762, 1129
RsaI GTAC 3 cut(s) 614, 729, 1113
RsaNI GTAC 3 cut(s) 613, 728, 1112
RseI CAYNNNNRTG 3 cut(s) 210, 369, 647
SacI GAGCTC 1 cut(s) 76
SapI GCTCTTC 1 cut(s) 1228
SaqAI TTAA 1 cut(s) 1064
SatI GCNGC 1 cut(s) 23
Sau3AI GATC 5 cut(s) 317, 762, 1093, 1129, 1219
Sau96I GGNCC 1 cut(s) 65
ScaI AGTACT 1 cut(s) 614
SchI GAGTC 1 cut(s) 1183
ScrFI CCNGG 4 cut(s) 69, 195, 663, 921
SduI GDGCHC 2 cut(s) 76, 93
SfaNI GCATC 2 cut(s) 632, 1012
SfcI CTRYAG 2 cut(s) 313, 431
SmiMI CAYNNNNRTG 3 cut(s) 210, 369, 647
SpeI ACTAGT 1 cut(s) 494
Sse9I AATT 7 cut(s) 420, 535, 792, 933, 968, 985, 1166
SseBI AGGCCT 1 cut(s) 1034
SsiI CCGC 3 cut(s) 147, 455, 558
SspMI CTAG 3 cut(s) 167, 495, 1282
SstI GAGCTC 1 cut(s) 76
StuI AGGCCT 1 cut(s) 1034
StyD4I CCNGG 4 cut(s) 67, 193, 661, 919
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 2 cut(s) 435, 649
TaiI ACGT 3 cut(s) 805, 945, 1090
TasI AATT 7 cut(s) 420, 535, 792, 933, 968, 985, 1166
TatI WGTACW 1 cut(s) 612
TfiI GAWTC 2 cut(s) 230, 1023
Tru1I TTAA 1 cut(s) 1064
Tru9I TTAA 1 cut(s) 1064
TscAI CASTG 3 cut(s) 181, 1001, 1189
TseFI GTSAC 2 cut(s) 888, 899
TseI GCWGC 1 cut(s) 22
Tsp45I GTSAC 2 cut(s) 888, 899
TspDTI ATGAA 7 cut(s) 194, 258, 471, 492, 548, 765, 935
TspRI CASTG 3 cut(s) 181, 1001, 1189
XapI RAATTY 4 cut(s) 535, 968, 985, 1166
XceI RCATGY 1 cut(s) 368
XmnI GAANNNNTTC 1 cut(s) 796
XspI CTAG 3 cut(s) 167, 495, 1282
ZrmI AGTACT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.