Rh4BG132400

DNA polymerase I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004379
Physical Location & Seq
Forward (+)
22329201 .. 22335566
6366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG132400.1

Sequence Viewer

Length: 534 bp
ATGGCCTGCTCTTTCTCTTCGCTGCTTCACTTTCAATCCCTTTGGAGAAGCTTCAAGTTGTTTGGCCCAGGAGCTCACAAGAGGCTCGGGCACCACTTGCGCACTCCCAACAAGCTCACTTGTCTCCATTTTTCTCCTATAACATCCGCAAAGGGACATTGTTATTTAGCCACCAGTGTTCACTCGGCATTACCCGGACTTGTTCATGACAATGGAGATTTGCTGGTTGATTCAGGGAAGCGTGAAGAGAAAATTGTAAATAGTAACCCTTCAAATGGTAGAGTAATGCTCATTGACGGCACGTCGATTATCTATAGATCATACTACAAGCTTCTTGCCAAGTTACACCATGGCCATCTGACACATGCTGATGGAAATGGAGATTGGGTGCTGACCATATTTACAGCGCTTTCTCTTATAATTGATGTGCTACAGTTTGTTCCTTCCCATGTGGCGGTGGTGTTTGACCATGATGGAGGTTCATTTGGTCAAACTAGTGTTTCATCCAAAGAAAGTGTTAGAGGAAAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_3_exonuc 5_3_exonuc_N

Protein Analysis

177

Amino Acids

19.35

Weight (kDa)

9.24

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_3_exonuc_N PF02739 95 - 162 4.8e-08 5'-3' exonuclease, N-terminal resolvase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 419
Acc16I TGCGCA 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 90
AciI CCGC 2 cut(s) 147, 455
AcoI YGGCCR 1 cut(s) 352
AfeI AGCGCT 1 cut(s) 408
AfiI CCNNNNNNNGG 2 cut(s) 275, 454
AgsI TTSAA 3 cut(s) 35, 55, 273
AhdI GACNNNNNGTC 1 cut(s) 301
AhlI ACTAGT 1 cut(s) 494
AjiI CACGTC 1 cut(s) 303
AjnI CCWGG 1 cut(s) 67
AjuI GAANNNNNNNTTGG 2 cut(s) 367, 399
AluBI AGCT 4 cut(s) 51, 74, 115, 331
AluI AGCT 4 cut(s) 51, 74, 115, 331
Alw21I GWGCWC 1 cut(s) 76
Alw26I GTCTC 1 cut(s) 128
Ama87I CYCGRG 1 cut(s) 86
Aor51HI AGCGCT 1 cut(s) 408
AoxI GGCC 3 cut(s) 3, 64, 352
ApeKI GCWGC 1 cut(s) 22
AspLEI GCGC 2 cut(s) 102, 409
AspS9I GGNCC 1 cut(s) 65
AsuC2I CCSGG 1 cut(s) 195
AvaI CYCGRG 1 cut(s) 86
BaeGI GKGCMC 1 cut(s) 93
BalI TGGCCA 1 cut(s) 354
BanI GGYRCC 1 cut(s) 90
BanII GRGCYC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 76
BbvI GCAGC 1 cut(s) 9
BccI CCATC 3 cut(s) 363, 365, 467
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 1 cut(s) 69
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 128
BcuI ACTAGT 1 cut(s) 494
BfaI CTAG 1 cut(s) 495
BfmI CTRYAG 2 cut(s) 313, 431
BfoI RGCGCY 1 cut(s) 410
BisI GCNGC 1 cut(s) 23
BlsI GCNGC 1 cut(s) 24
Bme1390I CCNGG 2 cut(s) 69, 195
BmeRI GACNNNNNGTC 1 cut(s) 301
BmeT110I CYCGRG 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 65
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 2 cut(s) 69, 195
BplI GAGNNNNNCTC 2 cut(s) 273, 305
BpuMI CCSGG 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 67, 349
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 454
Bse1I ACTGG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 69
BseDI CCNNGG 2 cut(s) 67, 349
BseGI GGATG 2 cut(s) 143, 503
BseLI CCNNNNNNNGG 2 cut(s) 275, 454
BseNI ACTGG 1 cut(s) 174
BseSI GKGCMC 1 cut(s) 93
BseXI GCAGC 1 cut(s) 9
BshFI GGCC 3 cut(s) 5, 66, 354
BshNI GGYRCC 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 76
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 2 cut(s) 275, 454
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 3 cut(s) 5, 66, 354
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 76, 93
Bsp143I GATC 1 cut(s) 317
Bsp19I CCATGG 1 cut(s) 349
BspACI CCGC 2 cut(s) 147, 455
BspANI GGCC 3 cut(s) 5, 66, 354
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 90
BsrI ACTGG 1 cut(s) 174
BssECI CCNNGG 2 cut(s) 67, 349
BssMI GATC 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 69
Bst4CI ACNGT 1 cut(s) 435
Bst6I CTCTTC 2 cut(s) 22, 240
BstAPI GCANNNNNTGC 1 cut(s) 97
BstC8I GCNNGC 1 cut(s) 7
BstDSI CCRYGG 1 cut(s) 349
BstF5I GGATG 2 cut(s) 143, 503
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 2 cut(s) 102, 409
BstKTI GATC 1 cut(s) 320
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 1 cut(s) 317
BstMWI GCNNNNNNNGC 1 cut(s) 97
BstNI CCWGG 1 cut(s) 69
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 67, 193
BstSFI CTRYAG 2 cut(s) 313, 431
BstSLI GKGCMC 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 9
BsuRI GGCC 3 cut(s) 5, 66, 354
BtgI CCRYGG 1 cut(s) 349
BtrI CACGTC 1 cut(s) 303
BtsCI GGATG 2 cut(s) 143, 503
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 1 cut(s) 7
CciI TCATGA 1 cut(s) 205
CfoI GCGC 2 cut(s) 102, 409
Cfr13I GGNCC 1 cut(s) 65
CviAII CATG 6 cut(s) 206, 350, 365, 449, 470, 531
CviJI RGCY 9 cut(s) 5, 51, 66, 74, 85, 115, 170, 331, 354
CviKI_1 RGCY 9 cut(s) 5, 51, 66, 74, 85, 115, 170, 331, 354
DpnI GATC 1 cut(s) 319
DpnII GATC 1 cut(s) 317
DriI GACNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 352
Eam1104I CTCTTC 2 cut(s) 22, 240
Eam1105I GACNNNNNGTC 1 cut(s) 301
EarI CTCTTC 2 cut(s) 22, 240
Ecl136II GAGCTC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 76
Eco47III AGCGCT 1 cut(s) 408
Eco53kI GAGCTC 1 cut(s) 74
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 76
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 6 cut(s) 209, 353, 368, 452, 473, 534
FaqI GGGAC 1 cut(s) 168
FatI CATG 6 cut(s) 205, 349, 364, 448, 469, 530
Fnu4HI GCNGC 1 cut(s) 23
FokI GGATG 2 cut(s) 130, 490
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 1 cut(s) 23
FspBI CTAG 1 cut(s) 495
FspI TGCGCA 1 cut(s) 101
GlaI GCGC 2 cut(s) 101, 408
GluI GCNGC 1 cut(s) 23
HaeII RGCGCY 1 cut(s) 410
HaeIII GGCC 3 cut(s) 5, 66, 354
HapII CCGG 1 cut(s) 195
HhaI GCGC 2 cut(s) 102, 409
Hin1II CATG 6 cut(s) 209, 353, 368, 452, 473, 534
Hin6I GCGC 2 cut(s) 100, 407
HinP1I GCGC 2 cut(s) 100, 407
HindIII AAGCTT 2 cut(s) 49, 329
HinfI GANTC 1 cut(s) 230
HpaII CCGG 1 cut(s) 195
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 2 cut(s) 279, 453
HpyCH4III ACNGT 1 cut(s) 435
HpyCH4IV ACGT 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 97
HpySE526I ACGT 1 cut(s) 302
Hsp92II CATG 6 cut(s) 209, 353, 368, 452, 473, 534
HspAI GCGC 2 cut(s) 100, 407
Kzo9I GATC 1 cut(s) 317
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 7 cut(s) 19, 54, 81, 187, 208, 209, 219
Lsp1109I GCAGC 1 cut(s) 9
MaeI CTAG 1 cut(s) 495
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 2 cut(s) 263, 342
MalI GATC 1 cut(s) 319
MboI GATC 1 cut(s) 317
MboII GAAGA 2 cut(s) 9, 257
MhlI GDGCHC 2 cut(s) 76, 93
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 2 cut(s) 252, 420
MluNI TGGCCA 1 cut(s) 354
MnlI CCTC 3 cut(s) 75, 470, 515
Mox20I TGGCCA 1 cut(s) 354
MscI TGGCCA 1 cut(s) 354
MslI CAYNNNNRTG 2 cut(s) 210, 369
Msp20I TGGCCA 1 cut(s) 354
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 2 cut(s) 69, 195
MvaI CCWGG 1 cut(s) 69
MwoI GCNNNNNNNGC 1 cut(s) 97
NciI CCSGG 1 cut(s) 195
NcoI CCATGG 1 cut(s) 349
NdeII GATC 1 cut(s) 317
NlaIII CATG 6 cut(s) 209, 353, 368, 452, 473, 534
NlaIV GGNNCC 1 cut(s) 92
NmeAIII GCCGAG 1 cut(s) 164
NsbI TGCGCA 1 cut(s) 101
NspI RCATGY 1 cut(s) 368
PagI TCATGA 1 cut(s) 205
PfeI GAWTC 1 cut(s) 230
PkrI GCNGC 1 cut(s) 24
PsiI TTATAA 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 67
PspGI CCWGG 1 cut(s) 67
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 1 cut(s) 65
RseI CAYNNNNRTG 2 cut(s) 210, 369
SacI GAGCTC 1 cut(s) 76
SatI GCNGC 1 cut(s) 23
Sau3AI GATC 1 cut(s) 317
Sau96I GGNCC 1 cut(s) 65
ScrFI CCNGG 2 cut(s) 69, 195
SduI GDGCHC 2 cut(s) 76, 93
SetI ASST 6 cut(s) 53, 76, 117, 305, 333, 481
SfcI CTRYAG 2 cut(s) 313, 431
SmiMI CAYNNNNRTG 2 cut(s) 210, 369
SpeI ACTAGT 1 cut(s) 494
Sse9I AATT 2 cut(s) 252, 420
SsiI CCGC 2 cut(s) 147, 455
SspMI CTAG 1 cut(s) 495
SstI GAGCTC 1 cut(s) 76
StyD4I CCNGG 2 cut(s) 67, 193
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 1 cut(s) 435
TaiI ACGT 1 cut(s) 305
TaqI TCGA 1 cut(s) 305
TasI AATT 2 cut(s) 252, 420
TfiI GAWTC 1 cut(s) 230
TscAI CASTG 1 cut(s) 181
TseI GCWGC 1 cut(s) 22
TspDTI ATGAA 3 cut(s) 194, 471, 492
TspRI CASTG 1 cut(s) 181
XceI RCATGY 1 cut(s) 368
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.