FvH4_5g12230

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003003
Physical Location & Seq
Forward (+)
6877431 .. 6881287
3857 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12230.t3

Sequence Viewer

Length: 1314 bp
ATGGAAGAAACCATCAACATACCGCGTCCCAAACGCAAACAGTCGGTAAACAATGAAGCAGAACAGCAACAGACACAGCCTGAGCATCACAAAAGGAGGCGCAGAACTTTGGAGTCCGAAACCGAAAAGCTAACCCGACAACCCGACCCGTCGTCTTCAAATAGTTTCCAACTCTTGGCCTGTACATCTCGGAACAAAGAAACAGAGAGCTATCGGGAATGGGTCTGCAACTCTTCTACTTCTTCTGCTTATGGAGAGAAGTTTGTGGTTGTGTCTTATAACATTCTGGGTGTTGATAACGCATCCAATCACTCAGATATGTATAGCAATGTGCCACCTAAATACATGAAGTGGAAGCGTCGTAGGAGGCTTATACGAAAAGAGGTCAACAGTTACAATGCTGGCATTGTGTGTTTTCAGGAGGTTGATCGTTTCGATGATTTAGCTCATAAGTTTCGGAAAGATGGGTTCGAAGGTGTGCACAAGGCACGTACAGGTGAAGCAAGTGATGGATGTGCTATATTTTGGAAGCAGGACATGTTTAGCCTTTTGCATCAAGAAAGTATAGAATTCCAAAGTTTTGGCCTTCGTCATAATGTTGCTCAATTTTGTGTTTTAAAGTTGCAAGATCAGTTAGAGTCGGATATACCTCCCGATGCATCAATGAGGCCATGTACCCAATGTAGAAGCTTAGTGGTTGGGAATATACATGTCCTATTCAACCCTAACCGTGGAGATATTAAACTAGGGCAGGTCCGACTGTTTCTTGAAAAGGCTTTCAAGCTATCACTAGAGTGGGGATCTATCCCTGTTATAGTTGCTGGAGACCTAAATAGTACTCCACAGAGTCCTATTTACCAATTTCTAGCTTCGTCTAAGTTAGACATCCAAAAACATGACCGCAGACATATTTCGGGACAGATGGAATTTGAATCTCAGTATAGAACCTTCATGTATAATGCAACCAGGTATGGAAAGCCATTCGTTTATAGATGGAAAGCAGAAGAAGTAAGGCTTGCAACTGGCTGTGAAGAAGTCACTCATCTTCAACACCATCTAAAGCTGGGCAGTGCATATCATGGAGTACCTGGTAGTAAGAGAACACGAGATAGGTGTGGAGAACCCTTGGCAACATCATACCACTCCAACTTTTTGGGAACTGTTGATTATATTTGGCATACAGAGGAACTTGTTCCGGTCAGAGTTCTTGAAACGTTACCCATCGACATTTTAAAAGGAACCAGTGGACTCCCCAGCAAGAGATGGGGGAGCGATCATCTTGCTCTTGTGTGTGAACTGGCTTTCCGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

438

Amino Acids

50.37

Weight (kDa)

8.95

Isoelectric Point (pI)

59.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 92 - 392 3e-14 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 742
AccB7I CCANNNNNTGG 1 cut(s) 175
AccII CGCG 1 cut(s) 25
AciI CCGC 2 cut(s) 23, 901
AclI AACGTT 1 cut(s) 1214
AclWI GGATC 1 cut(s) 808
AcsI RAATTY 2 cut(s) 569, 926
AfaI GTAC 5 cut(s) 184, 493, 676, 838, 1086
AfiI CCNNNNNNNGG 2 cut(s) 175, 731
AflIII ACRYGT 2 cut(s) 537, 709
AgsI TTSAA 7 cut(s) 159, 721, 770, 781, 932, 1049, 1211
AhdI GACNNNNNGTC 1 cut(s) 151
AjnI CCWGG 2 cut(s) 965, 1087
AleI CACNNNNGTG 1 cut(s) 793
AloI GAACNNNNNNTCC 1 cut(s) 1287
AluBI AGCT 7 cut(s) 130, 210, 446, 690, 784, 869, 1063
AluI AGCT 7 cut(s) 130, 210, 446, 690, 784, 869, 1063
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 1 cut(s) 819
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 1 cut(s) 808
AoxI GGCC 3 cut(s) 177, 583, 668
ApaLI GTGCAC 1 cut(s) 479
ApoI RAATTY 2 cut(s) 569, 926
Asp700I GAANNNNTTC 1 cut(s) 1191
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 1 cut(s) 754
AsuHPI GGTGA 1 cut(s) 509
AsuII TTCGAA 1 cut(s) 471
AvaII GGWCC 1 cut(s) 754
BaeGI GKGCMC 1 cut(s) 483
BarI GAAGNNNNNNTAC 2 cut(s) 932, 964
BauI CACGAG 1 cut(s) 1104
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 483
BccI CCATC 8 cut(s) 20, 458, 503, 916, 987, 1062, 1229, 1257
BcgI CGANNNNNNTGC 2 cut(s) 1262, 1296
BciT130I CCWGG 2 cut(s) 967, 1089
BcoDI GTCTC 1 cut(s) 819
BfaI CTAG 3 cut(s) 746, 791, 866
BfuAI ACCTGC 1 cut(s) 742
BmcAI AGTACT 1 cut(s) 838
Bme1390I CCNGG 2 cut(s) 967, 1089
Bme18I GGWCC 1 cut(s) 754
BmeRI GACNNNNNGTC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 754
BmiI GGNNCC 1 cut(s) 1240
BmrFI CCNGG 2 cut(s) 967, 1089
BmsI GCATC 5 cut(s) 94, 311, 562, 646, 668
BpiI GAAGAC 1 cut(s) 147
BpmI CTGGAG 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 81
Bpu14I TTCGAA 1 cut(s) 471
BsaAI YACGTR 1 cut(s) 491
BsaI GGTCTC 1 cut(s) 819
BsaJI CCNNGG 2 cut(s) 730, 1125
BsaWI WCCGGW 1 cut(s) 1195
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 731
Bse1I ACTGG 3 cut(s) 1027, 1242, 1302
Bse3DI GCAATG 1 cut(s) 334
BseBI CCWGG 2 cut(s) 967, 1089
BseDI CCNNGG 2 cut(s) 730, 1125
BseGI GGATG 3 cut(s) 302, 518, 885
BseLI CCNNNNNNNGG 2 cut(s) 175, 731
BseMI GCAATG 1 cut(s) 334
BseMII CTCAG 3 cut(s) 72, 327, 950
BseNI ACTGG 3 cut(s) 1027, 1242, 1302
BseSI GKGCMC 1 cut(s) 483
BseYI CCCAGC 2 cut(s) 1063, 1253
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 3 cut(s) 179, 585, 670
BsiHKAI GWGCWC 1 cut(s) 483
BsiSI CCGG 1 cut(s) 1196
BslFI GGGAC 2 cut(s) 12, 930
BslI CCNNNNNNNGG 2 cut(s) 175, 731
BsmAI GTCTC 1 cut(s) 819
BsmFI GGGAC 2 cut(s) 12, 930
BsnI GGCC 3 cut(s) 179, 585, 670
Bso31I GGTCTC 1 cut(s) 819
Bsp119I TTCGAA 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 483
Bsp1407I TGTACA 1 cut(s) 182
Bsp143I GATC 4 cut(s) 427, 628, 800, 1273
BspACI CCGC 2 cut(s) 23, 901
BspANI GGCC 3 cut(s) 179, 585, 670
BspCNI CTCAG 3 cut(s) 73, 326, 949
BspFNI CGCG 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 1240
BspMI ACCTGC 1 cut(s) 742
BspPI GGATC 1 cut(s) 808
BspT104I TTCGAA 1 cut(s) 471
BspTNI GGTCTC 1 cut(s) 819
BsrDI GCAATG 1 cut(s) 334
BsrGI TGTACA 1 cut(s) 182
BsrI ACTGG 3 cut(s) 1027, 1242, 1302
BssECI CCNNGG 2 cut(s) 730, 1125
BssMI GATC 4 cut(s) 427, 628, 800, 1273
BssSI CACGAG 1 cut(s) 1104
BssT1I CCWWGG 1 cut(s) 1125
Bst2BI CACGAG 1 cut(s) 1104
Bst2UI CCWGG 2 cut(s) 967, 1089
Bst4CI ACNGT 5 cut(s) 42, 392, 731, 762, 1162
Bst6I CTCTTC 1 cut(s) 238
BstAUI TGTACA 1 cut(s) 182
BstBAI YACGTR 1 cut(s) 491
BstBI TTCGAA 1 cut(s) 471
BstC8I GCNNGC 2 cut(s) 403, 1017
BstDEI CTNAG 5 cut(s) 81, 313, 691, 876, 936
BstDSI CCRYGG 1 cut(s) 730
BstF5I GGATG 3 cut(s) 302, 518, 885
BstFNI CGCG 1 cut(s) 25
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 4 cut(s) 430, 631, 803, 1276
BstMAI GTCTC 1 cut(s) 819
BstMBI GATC 4 cut(s) 427, 628, 800, 1273
BstNI CCWGG 2 cut(s) 967, 1089
BstNSI RCATGY 2 cut(s) 541, 713
BstSCI CCNGG 2 cut(s) 965, 1087
BstSLI GKGCMC 1 cut(s) 483
BstUI CGCG 1 cut(s) 25
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 800
BstXI CCANNNNNNTGG 2 cut(s) 581, 1153
BstYI RGATCY 1 cut(s) 800
BsuRI GGCC 3 cut(s) 179, 585, 670
BtgI CCRYGG 1 cut(s) 730
BtsCI GGATG 3 cut(s) 302, 518, 885
BtsI GCAGTG 1 cut(s) 1075
BtsIMutI CAGTG 2 cut(s) 1075, 1249
BveI ACCTGC 1 cut(s) 742
Cac8I GCNNGC 2 cut(s) 403, 1017
CfoI GCGC 1 cut(s) 102
Cfr13I GGNCC 1 cut(s) 754
CseI GACGC 2 cut(s) 14, 347
CsiI ACCWGGT 2 cut(s) 965, 1087
Csp6I GTAC 5 cut(s) 183, 492, 675, 837, 1085
CviAII CATG 7 cut(s) 346, 538, 672, 710, 896, 952, 1079
CviQI GTAC 5 cut(s) 183, 492, 675, 837, 1085
DdeI CTNAG 5 cut(s) 81, 313, 691, 876, 936
DpnI GATC 4 cut(s) 429, 630, 802, 1275
DpnII GATC 4 cut(s) 427, 628, 800, 1273
DraI TTTAAA 2 cut(s) 618, 1233
DriI GACNNNNNGTC 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 238
Eam1105I GACNNNNNGTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 1125
Eco31I GGTCTC 1 cut(s) 819
Eco47I GGWCC 1 cut(s) 754
EcoRI GAATTC 1 cut(s) 569
EcoRII CCWGG 2 cut(s) 965, 1087
EcoT14I CCWWGG 1 cut(s) 1125
EcoT22I ATGCAT 1 cut(s) 661
ErhI CCWWGG 1 cut(s) 1125
FaeI CATG 7 cut(s) 349, 541, 675, 713, 899, 955, 1082
FalI AAGNNNNNCTT 2 cut(s) 999, 1031
FaqI GGGAC 2 cut(s) 12, 930
FatI CATG 7 cut(s) 345, 537, 671, 709, 895, 951, 1078
FokI GGATG 3 cut(s) 289, 525, 872
FspBI CTAG 3 cut(s) 746, 791, 866
GlaI GCGC 1 cut(s) 101
GsaI CCCAGC 2 cut(s) 1067, 1257
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 3 cut(s) 179, 585, 670
HapII CCGG 1 cut(s) 1196
HgaI GACGC 2 cut(s) 14, 347
HhaI GCGC 1 cut(s) 102
Hin1II CATG 7 cut(s) 349, 541, 675, 713, 899, 955, 1082
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 688
HinfI GANTC 5 cut(s) 113, 638, 847, 932, 1248
HpaII CCGG 1 cut(s) 1196
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 5 cut(s) 49, 388, 481, 1247, 1295
Hpy188I TCNGA 7 cut(s) 118, 192, 316, 459, 643, 758, 1202
Hpy188III TCNNGA 7 cut(s) 215, 419, 557, 653, 767, 915, 1208
Hpy8I GTNNAC 5 cut(s) 49, 388, 481, 1247, 1295
Hpy99I CGWCG 2 cut(s) 154, 363
HpyAV CCTTC 3 cut(s) 467, 596, 958
HpyCH4III ACNGT 5 cut(s) 42, 392, 731, 762, 1162
HpyCH4IV ACGT 2 cut(s) 490, 1214
HpyCH4V TGCA 8 cut(s) 228, 481, 553, 625, 659, 962, 1019, 1073
HpyF3I CTNAG 5 cut(s) 81, 313, 691, 876, 936
HpySE526I ACGT 2 cut(s) 490, 1214
Hsp92II CATG 7 cut(s) 349, 541, 675, 713, 899, 955, 1082
HspAI GCGC 1 cut(s) 100
Kzo9I GATC 4 cut(s) 427, 628, 800, 1273
LmnI GCTCC 1 cut(s) 1269
LweI GCATC 5 cut(s) 94, 311, 562, 646, 668
MabI ACCWGGT 2 cut(s) 965, 1087
MaeI CTAG 3 cut(s) 746, 791, 866
MaeII ACGT 2 cut(s) 490, 1214
MaeIII GTNAC 3 cut(s) 392, 1036, 1215
MalI GATC 4 cut(s) 429, 630, 802, 1275
MboI GATC 4 cut(s) 427, 628, 800, 1273
MboII GAAGA 7 cut(s) 17, 147, 225, 234, 1016, 1037, 1043
MflI RGATCY 1 cut(s) 800
MhlI GDGCHC 1 cut(s) 483
MluCI AATT 4 cut(s) 569, 605, 860, 926
MlyI GAGTC 4 cut(s) 122, 647, 856, 1242
MmeI TCCRAC 4 cut(s) 193, 621, 781, 1170
MnlI CCTC 7 cut(s) 90, 360, 376, 415, 660, 660, 1177
Mph1103I ATGCAT 1 cut(s) 661
MroXI GAANNNNTTC 1 cut(s) 1191
MseI TTAA 3 cut(s) 617, 741, 1232
MslI CAYNNNNRTG 1 cut(s) 793
MspI CCGG 1 cut(s) 1196
MspR9I CCNGG 2 cut(s) 967, 1089
MvaI CCWGG 2 cut(s) 967, 1089
MvnI CGCG 1 cut(s) 25
NdeII GATC 4 cut(s) 427, 628, 800, 1273
NlaIII CATG 7 cut(s) 349, 541, 675, 713, 899, 955, 1082
NlaIV GGNNCC 1 cut(s) 1240
NmuCI GTSAC 1 cut(s) 1036
NsiI ATGCAT 1 cut(s) 661
NspI RCATGY 2 cut(s) 541, 713
NspV TTCGAA 1 cut(s) 471
OliI CACNNNNGTG 1 cut(s) 793
PciI ACATGT 2 cut(s) 537, 709
PdmI GAANNNNTTC 1 cut(s) 1191
PfeI GAWTC 1 cut(s) 932
PflMI CCANNNNNTGG 1 cut(s) 175
PleI GAGTC 4 cut(s) 121, 646, 855, 1242
PpsI GAGTC 4 cut(s) 121, 646, 855, 1242
Ppu21I YACGTR 1 cut(s) 491
PscI ACATGT 2 cut(s) 537, 709
PsiI TTATAA 1 cut(s) 279
Psp1406I AACGTT 1 cut(s) 1214
Psp6I CCWGG 2 cut(s) 965, 1087
PspFI CCCAGC 2 cut(s) 1063, 1253
PspGI CCWGG 2 cut(s) 965, 1087
PspN4I GGNNCC 1 cut(s) 1240
PspPI GGNCC 1 cut(s) 754
PsuI RGATCY 1 cut(s) 800
RsaI GTAC 5 cut(s) 184, 493, 676, 838, 1086
RsaNI GTAC 5 cut(s) 183, 492, 675, 837, 1085
RseI CAYNNNNRTG 1 cut(s) 793
SaqAI TTAA 3 cut(s) 617, 741, 1232
Sau3AI GATC 4 cut(s) 427, 628, 800, 1273
Sau96I GGNCC 1 cut(s) 754
ScaI AGTACT 1 cut(s) 838
SchI GAGTC 4 cut(s) 122, 647, 856, 1242
ScrFI CCNGG 2 cut(s) 967, 1089
SduI GDGCHC 1 cut(s) 483
SexAI ACCWGGT 2 cut(s) 965, 1087
SfaNI GCATC 5 cut(s) 94, 311, 562, 646, 668
SfuI TTCGAA 1 cut(s) 471
SinI GGWCC 1 cut(s) 754
SmiMI CAYNNNNRTG 1 cut(s) 793
Sse9I AATT 4 cut(s) 569, 605, 860, 926
SsiI CCGC 2 cut(s) 23, 901
SspMI CTAG 3 cut(s) 746, 791, 866
StyD4I CCNGG 2 cut(s) 965, 1087
StyI CCWWGG 1 cut(s) 1125
TaaI ACNGT 5 cut(s) 42, 392, 731, 762, 1162
TaiI ACGT 2 cut(s) 493, 1217
TaqI TCGA 3 cut(s) 435, 471, 1224
TasI AATT 4 cut(s) 569, 605, 860, 926
TatI WGTACW 2 cut(s) 182, 836
TfiI GAWTC 1 cut(s) 932
Tru1I TTAA 3 cut(s) 617, 741, 1232
Tru9I TTAA 3 cut(s) 617, 741, 1232
TscAI CASTG 2 cut(s) 1075, 1249
TseFI GTSAC 1 cut(s) 1036
Tsp45I GTSAC 1 cut(s) 1036
TspDTI ATGAA 3 cut(s) 69, 362, 940
TspGWI ACGGA 1 cut(s) 1295
TspRI CASTG 2 cut(s) 1075, 1249
Van91I CCANNNNNTGG 1 cut(s) 175
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 754
XapI RAATTY 2 cut(s) 569, 926
XceI RCATGY 2 cut(s) 541, 713
XmnI GAANNNNTTC 1 cut(s) 1191
XspI CTAG 3 cut(s) 746, 791, 866
ZrmI AGTACT 1 cut(s) 838
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.