MD06G1215600.v1.1

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003003
Physical Location & Seq
Reverse (-)
34795663 .. 34800285
4623 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1215600.v1.1.491

Sequence Viewer

Length: 1356 bp
ATGGAAGAAACGGGCGATGTACGGCGGCGTAAGCGCAAGCAACATTCACACAACACAGAAGCCGCAGAGAACCACCCTCACTCTCCGCCTCACGAAAGGCGGCGTCGCAGAAGAGTGAAGTCCGAATCTAAAACCCTAACCCGAGAATCCGACCCGCCACCGCCGCCGTCGTCATCCAGGTTCCAATTTGTATCGCCTTCGTATCGGAAGCACACGAAGCGGCAACCGAGTCGAACCGAGACAGAGAGCTCCCGGGAGTGGGCTTCTTCTCCCTGCAGCTCCTCTGCTTCCACCTATGGAGAGAAATTTGTGGTTGTTTCTTATAACATTCTGGGTGTGGAAAATGCATCCAAGCACCCAGAGTTGTACTACAAAGTCCCACCCATGTTCTTGGAGTGGAACCGCCGCCGGAAGCTTATTCGGAAGGAGATCAATCGTTACAATGCTAGCATCATGTGCTTGCAGGAGGTTGATCGTTTCGATGATTTAGCTCATCTTTTCCAGAAAGATGGGTTCGAAGGTGTTTATAAGGCGCGCACGGGTGAAGCAAATGATGGATGCGCCATATTTTGGAAAAAGGAAATGTTTAGCCTTTTACATGAAGAAAATATAGAGTTCCAAAGTTTTGGACTTCGTAACAATGTTGCTCAACTTTGTGTATTAAAGAGTAACCAAAATCAATTAGAGTCAGGCATACCTCCCGGAACGAGTCCATACACCCAAAGTAGAAGCTTAGTGATTGGGAATACACATCTACTATTCAACCCAAAACGTGGAGATATCAAGCTAGGGCAGGTCCGACTGTTTCTTAAAAAAGCTTACGAGCTATCACAAGAGTGGGGCAGCATCCCTGTTATAATTTCGGGAGATCTAAATAGCATCCCACAGAGTCCTATGTACCATTTCCTAGCTTCTTCTGAGTTGGACATCCGACTACATGACCGCAGAGGTATATCCGGACAGTTTGTCCTGAACTCGAATCACAGAGGCTTCAGATTTCAGAATGAGCAAGCAAGCAGGCCATTTTCATATATATGGAGCGAGGAAGAAGTAAGGCTTGCAACTGGCAGTGAAGTAGTCACTTGTCTTCGGCACAATTTGAAGCTTTGCAGTGCATACCTGGGAGTACCTGGTAGTTGTAGAACCAGAGATAGGTCTGGAGAACCCTTGGCAACATCATACCACTCCGAGTTTATGGGAACCGTTGATTATATTTGGCATACCGAGGAACTTGTTCCTGTCAGAGTTCTTGAAACCTTACCAGTCAATGCTTTAAGAAGAGATGGAGGACTCCCCAGCAAGCAATGGGGCAGCGATCATCTTGCTGTTGTATGCGAACTGGCTTTTCGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

452

Amino Acids

51.81

Weight (kDa)

9.28

Isoelectric Point (pI)

64.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 107 - 440 2.6e-16 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 324, 528, 857
Acc36I ACCTGC 1 cut(s) 784
AccB7I CCANNNNNTGG 2 cut(s) 570, 773
AccII CGCG 1 cut(s) 535
AccIII TCCGGA 1 cut(s) 956
AcsI RAATTY 1 cut(s) 305
AcuI CTGAAG 1 cut(s) 976
AcyI GRCGYC 1 cut(s) 103
AfaI GTAC 4 cut(s) 21, 368, 899, 1128
AfiI CCNNNNNNNGG 5 cut(s) 258, 259, 570, 773, 1152
AgsI TTSAA 3 cut(s) 763, 1102, 1253
AhdI GACNNNNNGTC 1 cut(s) 965
AjnI CCWGG 3 cut(s) 176, 1119, 1129
AleI CACNNNNGTG 1 cut(s) 835
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 233
Ama87I CYCGRG 2 cut(s) 141, 252
Aor13HI TCCGGA 1 cut(s) 956
AoxI GGCC 1 cut(s) 1019
ApeKI GCWGC 3 cut(s) 276, 843, 1311
ApoI RAATTY 1 cut(s) 305
Asp700I GAANNNNTTC 1 cut(s) 1233
AspLEI GCGC 4 cut(s) 36, 535, 537, 563
AspS9I GGNCC 1 cut(s) 796
AsuC2I CCSGG 3 cut(s) 253, 254, 702
AsuHPI GGTGA 1 cut(s) 554
AsuII TTCGAA 1 cut(s) 516
AsuNHI GCTAGC 1 cut(s) 446
AvaI CYCGRG 2 cut(s) 141, 252
AvaII GGWCC 1 cut(s) 796
BanII GRGCYC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 1079
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 3 cut(s) 288, 855, 1323
BccI CCATC 3 cut(s) 503, 548, 1277
BceAI ACGGC 2 cut(s) 38, 151
BcgI CGANNNNNNTGC 4 cut(s) 212, 246, 1304, 1338
BciT130I CCWGG 3 cut(s) 178, 1121, 1131
BcnI CCSGG 3 cut(s) 253, 254, 702
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 3 cut(s) 447, 788, 908
BfmI CTRYAG 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 784
BglII AGATCT 1 cut(s) 868
BisI GCNGC 9 cut(s) 26, 63, 101, 164, 221, 277, 406, 844, 1312
BlsI GCNGC 9 cut(s) 27, 64, 102, 165, 222, 278, 407, 845, 1313
Bme1390I CCNGG 6 cut(s) 178, 253, 254, 702, 1121, 1131
Bme18I GGWCC 1 cut(s) 796
BmeRI GACNNNNNGTC 1 cut(s) 965
BmeT110I CYCGRG 2 cut(s) 141, 252
BmgT120I GGNCC 1 cut(s) 796
BmiI GGNNCC 3 cut(s) 182, 401, 1201
BmrFI CCNGG 6 cut(s) 178, 253, 254, 702, 1121, 1131
BmsI GCATC 5 cut(s) 356, 459, 548, 855, 888
BmtI GCTAGC 1 cut(s) 450
BpiI GAAGAC 1 cut(s) 1079
BpmI CTGGAG 1 cut(s) 1179
Bpu14I TTCGAA 1 cut(s) 516
BpuMI CCSGG 3 cut(s) 253, 254, 702
BsaHI GRCGYC 1 cut(s) 103
BsaJI CCNNGG 4 cut(s) 252, 1120, 1167, 1224
BsaWI WCCGGW 1 cut(s) 956
Bsc4I CCNNNNNNNGG 5 cut(s) 258, 259, 570, 773, 1152
Bse1I ACTGG 3 cut(s) 1069, 1262, 1344
Bse3DI GCAATG 1 cut(s) 1310
BseAI TCCGGA 1 cut(s) 956
BseBI CCWGG 3 cut(s) 178, 1121, 1131
BseDI CCNNGG 4 cut(s) 252, 1120, 1167, 1224
BseGI GGATG 6 cut(s) 173, 347, 563, 846, 879, 927
BseLI CCNNNNNNNGG 5 cut(s) 258, 259, 570, 773, 1152
BseMI GCAATG 1 cut(s) 1310
BseMII CTCAG 1 cut(s) 909
BseNI ACTGG 3 cut(s) 1069, 1262, 1344
BsePI GCGCGC 1 cut(s) 533
BseRI GAGGAG 1 cut(s) 271
BseXI GCAGC 3 cut(s) 288, 855, 1323
BseYI CCCAGC 1 cut(s) 1295
Bsh1236I CGCG 1 cut(s) 535
BshFI GGCC 1 cut(s) 1021
BsiHKAI GWGCWC 1 cut(s) 251
BsiHKCI CYCGRG 2 cut(s) 141, 252
BsiSI CCGG 4 cut(s) 253, 409, 702, 957
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 5 cut(s) 258, 259, 570, 773, 1152
BsmAI GTCTC 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 1 cut(s) 1021
BsoBI CYCGRG 2 cut(s) 141, 252
Bsp119I TTCGAA 1 cut(s) 516
Bsp1286I GDGCHC 1 cut(s) 251
Bsp13I TCCGGA 1 cut(s) 956
Bsp143I GATC 4 cut(s) 429, 472, 868, 1315
BspANI GGCC 1 cut(s) 1021
BspCNI CTCAG 1 cut(s) 910
BspEI TCCGGA 1 cut(s) 956
BspFNI CGCG 1 cut(s) 535
BspLI GGNNCC 3 cut(s) 182, 401, 1201
BspMAI CTGCAG 1 cut(s) 278
BspMI ACCTGC 1 cut(s) 784
BspOI GCTAGC 1 cut(s) 450
BspT104I TTCGAA 1 cut(s) 516
BsrDI GCAATG 1 cut(s) 1310
BsrI ACTGG 3 cut(s) 1069, 1262, 1344
BssECI CCNNGG 4 cut(s) 252, 1120, 1167, 1224
BssHII GCGCGC 1 cut(s) 533
BssMI GATC 4 cut(s) 429, 472, 868, 1315
BssNI GRCGYC 1 cut(s) 103
BssT1I CCWWGG 1 cut(s) 1167
Bst2UI CCWGG 3 cut(s) 178, 1121, 1131
Bst4CI ACNGT 3 cut(s) 804, 963, 1204
Bst6I CTCTTC 2 cut(s) 106, 1273
BstACI GRCGYC 1 cut(s) 103
BstAPI GCANNNNNTGC 1 cut(s) 456
BstBI TTCGAA 1 cut(s) 516
BstC8I GCNNGC 9 cut(s) 38, 448, 461, 535, 1011, 1015, 1019, 1059, 1301
BstDEI CTNAG 2 cut(s) 733, 918
BstF5I GGATG 6 cut(s) 173, 347, 563, 846, 879, 927
BstFNI CGCG 1 cut(s) 535
BstHHI GCGC 4 cut(s) 36, 535, 537, 563
BstKTI GATC 4 cut(s) 432, 475, 871, 1318
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 4 cut(s) 429, 472, 868, 1315
BstMWI GCNNNNNNNGC 4 cut(s) 31, 163, 217, 456
BstNI CCWGG 3 cut(s) 178, 1121, 1131
BstSCI CCNGG 6 cut(s) 176, 251, 252, 700, 1119, 1129
BstSFI CTRYAG 1 cut(s) 274
BstUI CGCG 1 cut(s) 535
BstV1I GCAGC 3 cut(s) 288, 855, 1323
BstV2I GAAGAC 1 cut(s) 1079
BstX2I RGATCY 1 cut(s) 868
BstXI CCANNNNNNTGG 3 cut(s) 391, 509, 626
BstYI RGATCY 1 cut(s) 868
BsuRI GGCC 1 cut(s) 1021
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 6 cut(s) 173, 347, 563, 846, 879, 927
BtsI GCAGTG 2 cut(s) 1075, 1117
BtsIMutI CAGTG 2 cut(s) 1075, 1117
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 9 cut(s) 38, 448, 461, 535, 1011, 1015, 1019, 1059, 1301
CfoI GCGC 4 cut(s) 36, 535, 537, 563
Cfr13I GGNCC 1 cut(s) 796
Cfr9I CCCGGG 1 cut(s) 252
CseI GACGC 1 cut(s) 92
CsiI ACCWGGT 1 cut(s) 1129
Csp6I GTAC 4 cut(s) 20, 367, 898, 1127
CviAII CATG 4 cut(s) 385, 454, 599, 938
CviQI GTAC 4 cut(s) 20, 367, 898, 1127
DdeI CTNAG 2 cut(s) 733, 918
DpnI GATC 4 cut(s) 431, 474, 870, 1317
DpnII GATC 4 cut(s) 429, 472, 868, 1315
DriI GACNNNNNGTC 1 cut(s) 965
Eam1104I CTCTTC 2 cut(s) 106, 1273
Eam1105I GACNNNNNGTC 1 cut(s) 965
EarI CTCTTC 2 cut(s) 106, 1273
EciI GGCGGA 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 1167
Eco24I GRGCYC 1 cut(s) 251
Eco32I GATATC 1 cut(s) 781
Eco47I GGWCC 1 cut(s) 796
Eco53kI GAGCTC 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 976
Eco88I CYCGRG 2 cut(s) 141, 252
EcoICRI GAGCTC 1 cut(s) 249
EcoRII CCWGG 3 cut(s) 176, 1119, 1129
EcoRV GATATC 1 cut(s) 781
EcoT14I CCWWGG 1 cut(s) 1167
EcoT22I ATGCAT 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 251
ErhI CCWWGG 1 cut(s) 1167
FaeI CATG 4 cut(s) 388, 457, 602, 941
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FaqI GGGAC 1 cut(s) 362
FatI CATG 4 cut(s) 384, 453, 598, 937
FauI CCCGC 1 cut(s) 162
Fnu4HI GCNGC 9 cut(s) 26, 63, 101, 164, 221, 277, 406, 844, 1312
FokI GGATG 6 cut(s) 160, 334, 570, 833, 866, 914
FriOI GRGCYC 1 cut(s) 251
Fsp4HI GCNGC 9 cut(s) 26, 63, 101, 164, 221, 277, 406, 844, 1312
FspBI CTAG 3 cut(s) 447, 788, 908
GlaI GCGC 4 cut(s) 35, 534, 536, 562
GluI GCNGC 9 cut(s) 26, 63, 101, 164, 221, 277, 406, 844, 1312
GsaI CCCAGC 1 cut(s) 1299
GsuI CTGGAG 1 cut(s) 1179
HaeIII GGCC 1 cut(s) 1021
HapII CCGG 4 cut(s) 253, 409, 702, 957
HgaI GACGC 1 cut(s) 92
HhaI GCGC 4 cut(s) 36, 535, 537, 563
Hin1I GRCGYC 1 cut(s) 103
Hin1II CATG 4 cut(s) 388, 457, 602, 941
Hin6I GCGC 4 cut(s) 34, 533, 535, 561
HinP1I GCGC 4 cut(s) 34, 533, 535, 561
HindIII AAGCTT 4 cut(s) 413, 730, 816, 1103
HinfI GANTC 8 cut(s) 125, 146, 229, 686, 709, 889, 979, 1290
HpaII CCGG 4 cut(s) 253, 409, 702, 957
HphI GGTGA 1 cut(s) 554
Hpy188III TCNNGA 7 cut(s) 92, 502, 864, 957, 970, 1158, 1250
Hpy99I CGWCG 2 cut(s) 108, 172
HpyAV CCTTC 3 cut(s) 207, 418, 512
HpyCH4III ACNGT 3 cut(s) 804, 963, 1204
HpyCH4IV ACGT 1 cut(s) 772
HpyCH4V TGCA 6 cut(s) 276, 347, 463, 1061, 1110, 1115
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 163, 217, 456
HpyF3I CTNAG 2 cut(s) 733, 918
HpySE526I ACGT 1 cut(s) 772
Hsp92I GRCGYC 1 cut(s) 103
Hsp92II CATG 4 cut(s) 388, 457, 602, 941
HspAI GCGC 4 cut(s) 34, 533, 535, 561
Kpn2I TCCGGA 1 cut(s) 956
Kzo9I GATC 4 cut(s) 429, 472, 868, 1315
LmnI GCTCC 3 cut(s) 254, 284, 1038
Lsp1109I GCAGC 3 cut(s) 288, 855, 1323
LweI GCATC 5 cut(s) 356, 459, 548, 855, 888
MabI ACCWGGT 1 cut(s) 1129
MaeI CTAG 3 cut(s) 447, 788, 908
MaeII ACGT 1 cut(s) 772
MaeIII GTNAC 4 cut(s) 437, 635, 668, 1078
MalI GATC 4 cut(s) 431, 474, 870, 1317
MboI GATC 4 cut(s) 429, 472, 868, 1315
MboII GAAGA 8 cut(s) 17, 123, 258, 614, 906, 1058, 1079, 1290
MflI RGATCY 1 cut(s) 868
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 5 cut(s) 185, 305, 680, 858, 1096
MlyI GAGTC 5 cut(s) 238, 695, 718, 898, 1284
MmeI TCCRAC 4 cut(s) 174, 823, 903, 955
Mph1103I ATGCAT 1 cut(s) 349
MroI TCCGGA 1 cut(s) 956
MroXI GAANNNNTTC 1 cut(s) 1233
MseI TTAA 3 cut(s) 662, 810, 1274
MslI CAYNNNNRTG 2 cut(s) 835, 1033
MspI CCGG 4 cut(s) 253, 409, 702, 957
MspR9I CCNGG 6 cut(s) 178, 253, 254, 702, 1121, 1131
MvaI CCWGG 3 cut(s) 178, 1121, 1131
MvnI CGCG 1 cut(s) 535
MwoI GCNNNNNNNGC 4 cut(s) 31, 163, 217, 456
NciI CCSGG 3 cut(s) 253, 254, 702
NdeII GATC 4 cut(s) 429, 472, 868, 1315
NheI GCTAGC 1 cut(s) 446
NlaIII CATG 4 cut(s) 388, 457, 602, 941
NlaIV GGNNCC 3 cut(s) 182, 401, 1201
NmuCI GTSAC 1 cut(s) 1078
NsiI ATGCAT 1 cut(s) 349
NspV TTCGAA 1 cut(s) 516
OliI CACNNNNGTG 1 cut(s) 835
PauI GCGCGC 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 1233
PfeI GAWTC 3 cut(s) 125, 146, 979
PflMI CCANNNNNTGG 2 cut(s) 570, 773
PfoI TCCNGGA 1 cut(s) 700
PkrI GCNGC 9 cut(s) 27, 64, 102, 165, 222, 278, 407, 845, 1313
PleI GAGTC 5 cut(s) 237, 694, 717, 897, 1284
PpsI GAGTC 5 cut(s) 237, 694, 717, 897, 1284
PsiI TTATAA 3 cut(s) 324, 528, 857
Psp124BI GAGCTC 1 cut(s) 251
Psp6I CCWGG 3 cut(s) 176, 1119, 1129
PspFI CCCAGC 1 cut(s) 1295
PspGI CCWGG 3 cut(s) 176, 1119, 1129
PspN4I GGNNCC 3 cut(s) 182, 401, 1201
PspPI GGNCC 1 cut(s) 796
PstI CTGCAG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 868
PteI GCGCGC 1 cut(s) 533
RsaI GTAC 4 cut(s) 21, 368, 899, 1128
RsaNI GTAC 4 cut(s) 20, 367, 898, 1127
RseI CAYNNNNRTG 2 cut(s) 835, 1033
SacI GAGCTC 1 cut(s) 251
SaqAI TTAA 3 cut(s) 662, 810, 1274
SatI GCNGC 9 cut(s) 26, 63, 101, 164, 221, 277, 406, 844, 1312
Sau3AI GATC 4 cut(s) 429, 472, 868, 1315
Sau96I GGNCC 1 cut(s) 796
SchI GAGTC 5 cut(s) 238, 695, 718, 898, 1284
ScrFI CCNGG 6 cut(s) 178, 253, 254, 702, 1121, 1131
SduI GDGCHC 1 cut(s) 251
SexAI ACCWGGT 1 cut(s) 1129
SfaNI GCATC 5 cut(s) 356, 459, 548, 855, 888
SfcI CTRYAG 1 cut(s) 274
SfuI TTCGAA 1 cut(s) 516
SinI GGWCC 1 cut(s) 796
SmaI CCCGGG 1 cut(s) 254
SmiMI CAYNNNNRTG 2 cut(s) 835, 1033
Sse9I AATT 5 cut(s) 185, 305, 680, 858, 1096
SspMI CTAG 3 cut(s) 447, 788, 908
SstI GAGCTC 1 cut(s) 251
StyD4I CCNGG 6 cut(s) 176, 251, 252, 700, 1119, 1129
StyI CCWWGG 1 cut(s) 1167
TaaI ACNGT 3 cut(s) 804, 963, 1204
TaiI ACGT 1 cut(s) 775
TaqI TCGA 4 cut(s) 232, 480, 516, 977
TasI AATT 5 cut(s) 185, 305, 680, 858, 1096
TatI WGTACW 1 cut(s) 366
TauI GCSGC 6 cut(s) 28, 65, 103, 166, 223, 408
TfiI GAWTC 3 cut(s) 125, 146, 979
Tru1I TTAA 3 cut(s) 662, 810, 1274
Tru9I TTAA 3 cut(s) 662, 810, 1274
TscAI CASTG 2 cut(s) 1075, 1117
TseFI GTSAC 1 cut(s) 1078
TseI GCWGC 3 cut(s) 276, 843, 1311
Tsp45I GTSAC 1 cut(s) 1078
TspDTI ATGAA 2 cut(s) 615, 1017
TspMI CCCGGG 1 cut(s) 252
TspRI CASTG 2 cut(s) 1075, 1117
Van91I CCANNNNNTGG 2 cut(s) 570, 773
VpaK11BI GGWCC 1 cut(s) 796
XapI RAATTY 1 cut(s) 305
XmaI CCCGGG 1 cut(s) 252
XmnI GAANNNNTTC 1 cut(s) 1233
XspI CTAG 3 cut(s) 447, 788, 908
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.