Rh7DG073300

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0003003
Physical Location & Seq
Reverse (-)
5671190 .. 5675155
3966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG073300.1

Sequence Viewer

Length: 1296 bp
ATGGAAGAAACCATCAACGTACTGCGTCCCAAGCGCAAACATTCACTAAACACTGAAGCAGAACAACCTGAGCCTCACAAAAGGAGGCGCAGAACTGTGGAGTCCGAAACCGAACCGCTAACCCGACAACCCGACCCGTCATCTTCAAATAGGTTCCGACTCTTGTCTTGTCCATCTCCCAAGAAAGAAACACAGAGCTCTCGGGAATGGGTCTGCAACTCTTCCACTTCTTCTGCCTATGGAGAGAAGTTTGTGGTTGTTTCTTATAACATTCTGGGTGTTGAAAATGCATCCAATCACTCAGATATGTATAATAATGTGCCACCCAGATACATGAAGTGGAAACGTCGCAGGAGGCTTATACGAAAGGAGGTCAAGAGTTACAATGCTAGCATTGTATGTTTTCAGGAGGTCGATCGTTTCGATGATTTAGCTCATAAGTTCCGGAAAGATGGGTTGGAAGGTGTGTACAAGGCACGCACTGGTGAAGTAAGTGATGGATGTGCTATATTTTGGAAACAGGACATGTTTAGCCTTTTGCATCAAGAAAGTATAGAGTTCCAAAGTTTTGGCCTTCGTCATAATGTTGCTCAATTTTGTGTTTTAAAGTTGCAAGATCAATTAGAGTCAGATGTACCTCCTGATACATCAATGAGGCCATGTACCCGAAGTAGAAGCTTAGTGATTGGGAATATACATGTCCTATTCAACCCTAGCCGTGGAGATATCAAGCTAGGGCAGGTTCGACTGTTTCTTGAAAAAGCTTACAAGCTATCACTAGAGTGGGGATCTATCCCTGTTATAATTGCTGGGGATCTAAATAGCACTCCACAGAGTCCTATTTACCAGTTTCTAGCTTCATCTCAGTTAGACATCCAACGACATGACCGCAGACGTGTCTCAGGACAGATTGAATTTGAATCGCAGTACAGAACCTTCACGTATAACAGGTATGGAAAGCCATTCTTTTATAGATGGAGTGCGGAAGAAGTAAGGCTTGCAACGGGCTGTGAAGAAGTCACTCATCTCCAACACCATCTAAAGCTTGGCAGTGCATATCTTGGAGTACCTGGTAGTCGCAGAACAAGAGATAGGTGTGGAGAACCCTTGGCAACATCATACCACTCCAAGTTTTTGGGAACTGTTGATTATATATGGCATACGGAGGAGCTTGTTCCGGTCAGAGTTCTTGAAACGTTACCCATCGACACTTTAAAAGGAATGGGAGGACTCCCCAGCAAGAGATGTGGGAGCGATCATCTTGCTCTTGTATGTGAACTGGCTTTCCGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

431

Amino Acids

49.72

Weight (kDa)

9.24

Isoelectric Point (pI)

64.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 88 - 420 1.3e-18 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 267, 803
Acc36I ACCTGC 1 cut(s) 730
AccIII TCCGGA 1 cut(s) 444
AciI CCGC 3 cut(s) 116, 889, 983
AclI AACGTT 1 cut(s) 1196
AclWI GGATC 2 cut(s) 796, 822
AcsI RAATTY 1 cut(s) 914
AcuI CTGAAG 1 cut(s) 75
AfaI GTAC 6 cut(s) 21, 470, 636, 664, 929, 1068
AfiI CCNNNNNNNGG 1 cut(s) 719
AflIII ACRYGT 3 cut(s) 525, 697, 895
AgsI TTSAA 7 cut(s) 147, 284, 709, 758, 914, 920, 1193
AjiI CACGTC 1 cut(s) 896
AjnI CCWGG 1 cut(s) 1069
AjuI GAANNNNNNNTTGG 2 cut(s) 440, 472
AleI CACNNNNGTG 1 cut(s) 781
AloI GAACNNNNNNTCC 1 cut(s) 1269
AluBI AGCT 9 cut(s) 198, 434, 678, 733, 764, 772, 857, 1045, 1171
AluI AGCT 9 cut(s) 198, 434, 678, 733, 764, 772, 857, 1045, 1171
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 1 cut(s) 904
AlwI GGATC 2 cut(s) 796, 822
Ama87I CYCGRG 1 cut(s) 201
Aor13HI TCCGGA 1 cut(s) 444
AoxI GGCC 2 cut(s) 571, 656
ApoI RAATTY 1 cut(s) 914
AspLEI GCGC 2 cut(s) 36, 90
AsuHPI GGTGA 1 cut(s) 497
AsuNHI GCTAGC 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 201
BanII GRGCYC 1 cut(s) 200
BarI GAAGNNNNNNTAC 2 cut(s) 920, 952
Bbv12I GWGCWC 1 cut(s) 200
BccI CCATC 7 cut(s) 20, 181, 446, 491, 969, 1044, 1211
BceAI ACGGC 1 cut(s) 702
BcgI CGANNNNNNTGC 2 cut(s) 1244, 1278
BciT130I CCWGG 1 cut(s) 1071
BcoDI GTCTC 1 cut(s) 904
BfaI CTAG 5 cut(s) 390, 714, 734, 779, 854
BfuAI ACCTGC 1 cut(s) 730
Bme1390I CCNGG 1 cut(s) 1071
BmeT110I CYCGRG 1 cut(s) 201
BmgBI CACGTC 1 cut(s) 896
BmiI GGNNCC 1 cut(s) 155
BmrFI CCNGG 1 cut(s) 1071
BmsI GCATC 2 cut(s) 299, 550
BmtI GCTAGC 1 cut(s) 393
BoxI GACNNNNGTC 1 cut(s) 163
Bpu10I CCTNAGC 1 cut(s) 69
BsaAI YACGTR 1 cut(s) 942
BsaJI CCNNGG 2 cut(s) 718, 1107
BsaWI WCCGGW 2 cut(s) 444, 1177
Bsc4I CCNNNNNNNGG 1 cut(s) 719
Bse1I ACTGG 3 cut(s) 487, 847, 1284
BseAI TCCGGA 1 cut(s) 444
BseBI CCWGG 1 cut(s) 1071
BseDI CCNNGG 2 cut(s) 718, 1107
BseGI GGATG 3 cut(s) 290, 506, 873
BseLI CCNNNNNNNGG 1 cut(s) 719
BseMII CTCAG 4 cut(s) 60, 315, 878, 915
BseNI ACTGG 3 cut(s) 487, 847, 1284
BseRI GAGGAG 1 cut(s) 1181
BseYI CCCAGC 2 cut(s) 809, 1235
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 2 cut(s) 573, 658
BsiEI CGRYCG 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 201
BsiSI CCGG 2 cut(s) 445, 1178
BslFI GGGAC 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 719
BsmAI GTCTC 1 cut(s) 904
BsmFI GGGAC 1 cut(s) 12
BsnI GGCC 2 cut(s) 573, 658
BsoBI CYCGRG 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 200
Bsp13I TCCGGA 1 cut(s) 444
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 5 cut(s) 415, 616, 788, 814, 1255
BspACI CCGC 3 cut(s) 116, 889, 983
BspANI GGCC 2 cut(s) 573, 658
BspCNI CTCAG 4 cut(s) 61, 314, 877, 914
BspEI TCCGGA 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 155
BspMI ACCTGC 1 cut(s) 730
BspOI GCTAGC 1 cut(s) 393
BspPI GGATC 2 cut(s) 796, 822
BsrGI TGTACA 1 cut(s) 468
BsrI ACTGG 3 cut(s) 487, 847, 1284
BssECI CCNNGG 2 cut(s) 718, 1107
BssMI GATC 5 cut(s) 415, 616, 788, 814, 1255
BssT1I CCWWGG 1 cut(s) 1107
Bst2UI CCWGG 1 cut(s) 1071
Bst4CI ACNGT 3 cut(s) 97, 750, 1144
Bst6I CTCTTC 1 cut(s) 226
BstAUI TGTACA 1 cut(s) 468
BstBAI YACGTR 1 cut(s) 942
BstC8I GCNNGC 3 cut(s) 391, 478, 999
BstDEI CTNAG 5 cut(s) 69, 301, 679, 864, 901
BstDSI CCRYGG 1 cut(s) 718
BstF5I GGATG 3 cut(s) 290, 506, 873
BstHHI GCGC 2 cut(s) 36, 90
BstKTI GATC 5 cut(s) 418, 619, 791, 817, 1258
BstMAI GTCTC 1 cut(s) 904
BstMBI GATC 5 cut(s) 415, 616, 788, 814, 1255
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstNI CCWGG 1 cut(s) 1071
BstNSI RCATGY 2 cut(s) 529, 701
BstPAI GACNNNNGTC 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 1069
BstX2I RGATCY 2 cut(s) 788, 814
BstXI CCANNNNNNTGG 2 cut(s) 569, 1135
BstYI RGATCY 2 cut(s) 788, 814
BsuRI GGCC 2 cut(s) 573, 658
BtgI CCRYGG 1 cut(s) 718
BtrI CACGTC 1 cut(s) 896
BtsCI GGATG 3 cut(s) 290, 506, 873
BtsI GCAGTG 1 cut(s) 1057
BtsIMutI CAGTG 3 cut(s) 51, 480, 1057
BveI ACCTGC 1 cut(s) 730
Cac8I GCNNGC 3 cut(s) 391, 478, 999
CfoI GCGC 2 cut(s) 36, 90
CseI GACGC 1 cut(s) 14
CsiI ACCWGGT 1 cut(s) 1069
Csp6I GTAC 6 cut(s) 20, 469, 635, 663, 928, 1067
CspCI CAANNNNNGTGG 2 cut(s) 1228, 1263
CviAII CATG 5 cut(s) 334, 526, 660, 698, 884
CviQI GTAC 6 cut(s) 20, 469, 635, 663, 928, 1067
DdeI CTNAG 5 cut(s) 69, 301, 679, 864, 901
DpnI GATC 5 cut(s) 417, 618, 790, 816, 1257
DpnII GATC 5 cut(s) 415, 616, 788, 814, 1255
DraI TTTAAA 2 cut(s) 606, 1215
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Ecl136II GAGCTC 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 1107
Eco24I GRGCYC 1 cut(s) 200
Eco32I GATATC 1 cut(s) 727
Eco53kI GAGCTC 1 cut(s) 198
Eco57I CTGAAG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 201
EcoICRI GAGCTC 1 cut(s) 198
EcoRII CCWGG 1 cut(s) 1069
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 1 cut(s) 1107
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 1107
FaeI CATG 5 cut(s) 337, 529, 663, 701, 887
FalI AAGNNNNNCTT 4 cut(s) 950, 982, 981, 1013
FaqI GGGAC 1 cut(s) 12
FatI CATG 5 cut(s) 333, 525, 659, 697, 883
FokI GGATG 3 cut(s) 277, 513, 860
FriOI GRGCYC 1 cut(s) 200
FspBI CTAG 5 cut(s) 390, 714, 734, 779, 854
GlaI GCGC 2 cut(s) 35, 89
GsaI CCCAGC 2 cut(s) 813, 1239
HaeIII GGCC 2 cut(s) 573, 658
HapII CCGG 2 cut(s) 445, 1178
HgaI GACGC 1 cut(s) 14
HhaI GCGC 2 cut(s) 36, 90
Hin1II CATG 5 cut(s) 337, 529, 663, 701, 887
Hin6I GCGC 2 cut(s) 34, 88
HinP1I GCGC 2 cut(s) 34, 88
HindIII AAGCTT 3 cut(s) 676, 762, 1043
HinfI GANTC 6 cut(s) 101, 159, 626, 835, 920, 1230
HpaII CCGG 2 cut(s) 445, 1178
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 2 cut(s) 469, 1277
Hpy188I TCNGA 5 cut(s) 106, 158, 304, 631, 1184
Hpy188III TCNNGA 9 cut(s) 203, 376, 407, 445, 545, 641, 755, 903, 1190
Hpy8I GTNNAC 2 cut(s) 469, 1277
Hpy99I CGWCG 1 cut(s) 351
HpyAV CCTTC 3 cut(s) 455, 584, 946
HpyCH4III ACNGT 3 cut(s) 97, 750, 1144
HpyCH4IV ACGT 5 cut(s) 18, 346, 895, 941, 1196
HpyCH4V TGCA 6 cut(s) 216, 290, 541, 613, 1001, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 5 cut(s) 69, 301, 679, 864, 901
HpySE526I ACGT 5 cut(s) 18, 346, 895, 941, 1196
Hsp92II CATG 5 cut(s) 337, 529, 663, 701, 887
HspAI GCGC 2 cut(s) 34, 88
Kpn2I TCCGGA 1 cut(s) 444
Kzo9I GATC 5 cut(s) 415, 616, 788, 814, 1255
LmnI GCTCC 2 cut(s) 1168, 1251
LweI GCATC 2 cut(s) 299, 550
MabI ACCWGGT 1 cut(s) 1069
MaeI CTAG 5 cut(s) 390, 714, 734, 779, 854
MaeII ACGT 5 cut(s) 18, 346, 895, 941, 1196
MaeIII GTNAC 3 cut(s) 380, 1018, 1197
MalI GATC 5 cut(s) 417, 618, 790, 816, 1257
MboI GATC 5 cut(s) 415, 616, 788, 814, 1255
MboII GAAGA 6 cut(s) 17, 135, 213, 222, 998, 1025
MflI RGATCY 2 cut(s) 788, 814
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 4 cut(s) 593, 620, 804, 914
MlyI GAGTC 5 cut(s) 110, 153, 635, 844, 1224
MmeI TCCRAC 4 cut(s) 181, 438, 901, 1054
MnlI CCTC 9 cut(s) 78, 84, 348, 364, 403, 648, 648, 1159, 1220
Mph1103I ATGCAT 1 cut(s) 292
MroI TCCGGA 1 cut(s) 444
MseI TTAA 2 cut(s) 605, 1214
MslI CAYNNNNRTG 1 cut(s) 781
MspI CCGG 2 cut(s) 445, 1178
MspR9I CCNGG 1 cut(s) 1071
MvaI CCWGG 1 cut(s) 1071
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeII GATC 5 cut(s) 415, 616, 788, 814, 1255
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 5 cut(s) 337, 529, 663, 701, 887
NlaIV GGNNCC 1 cut(s) 155
NmuCI GTSAC 1 cut(s) 1018
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 2 cut(s) 529, 701
OliI CACNNNNGTG 1 cut(s) 781
PciI ACATGT 2 cut(s) 525, 697
PcsI WCGNNNNNNNCGW 1 cut(s) 420
PfeI GAWTC 1 cut(s) 920
Ple19I CGATCG 1 cut(s) 418
PleI GAGTC 5 cut(s) 109, 153, 634, 843, 1224
PpsI GAGTC 5 cut(s) 109, 153, 634, 843, 1224
Ppu21I YACGTR 1 cut(s) 942
PscI ACATGT 2 cut(s) 525, 697
PshAI GACNNNNGTC 1 cut(s) 163
PsiI TTATAA 2 cut(s) 267, 803
Psp124BI GAGCTC 1 cut(s) 200
Psp1406I AACGTT 1 cut(s) 1196
Psp6I CCWGG 1 cut(s) 1069
PspFI CCCAGC 2 cut(s) 809, 1235
PspGI CCWGG 1 cut(s) 1069
PspN4I GGNNCC 1 cut(s) 155
PsuI RGATCY 2 cut(s) 788, 814
PvuI CGATCG 1 cut(s) 418
RsaI GTAC 6 cut(s) 21, 470, 636, 664, 929, 1068
RsaNI GTAC 6 cut(s) 20, 469, 635, 663, 928, 1067
RseI CAYNNNNRTG 1 cut(s) 781
SacI GAGCTC 1 cut(s) 200
SaqAI TTAA 2 cut(s) 605, 1214
Sau3AI GATC 5 cut(s) 415, 616, 788, 814, 1255
SchI GAGTC 5 cut(s) 110, 153, 635, 844, 1224
ScrFI CCNGG 1 cut(s) 1071
SduI GDGCHC 1 cut(s) 200
SexAI ACCWGGT 1 cut(s) 1069
SfaNI GCATC 2 cut(s) 299, 550
SmiMI CAYNNNNRTG 1 cut(s) 781
Sse9I AATT 4 cut(s) 593, 620, 804, 914
SsiI CCGC 3 cut(s) 116, 889, 983
SspMI CTAG 5 cut(s) 390, 714, 734, 779, 854
SstI GAGCTC 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 1069
StyI CCWWGG 1 cut(s) 1107
TaaI ACNGT 3 cut(s) 97, 750, 1144
TaiI ACGT 5 cut(s) 21, 349, 898, 944, 1199
TaqI TCGA 4 cut(s) 414, 423, 745, 1206
TasI AATT 4 cut(s) 593, 620, 804, 914
TatI WGTACW 2 cut(s) 468, 927
TfiI GAWTC 1 cut(s) 920
Tru1I TTAA 2 cut(s) 605, 1214
Tru9I TTAA 2 cut(s) 605, 1214
TscAI CASTG 3 cut(s) 58, 487, 1057
TseFI GTSAC 1 cut(s) 1018
Tsp45I GTSAC 1 cut(s) 1018
TspDTI ATGAA 2 cut(s) 350, 849
TspGWI ACGGA 2 cut(s) 1178, 1277
TspRI CASTG 3 cut(s) 58, 487, 1057
XapI RAATTY 1 cut(s) 914
XceI RCATGY 2 cut(s) 529, 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 1043
XspI CTAG 5 cut(s) 390, 714, 734, 779, 854
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.