Rh7CG073700

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0003003
Physical Location & Seq
Reverse (-)
5265985 .. 5270208
4224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG073700.1

Sequence Viewer

Length: 972 bp
ATGGAAGAAACCATCAACGTACTGCGTCCCAGGCGCAAACATTCACTAAACACTGAAGCAGAACAACCTGAGCCTCACAAAAGGAGGCGCAGAACTGTGGAGTCCGAAACCGAACCGCTAACCCGACAACCCGACCCGTCATCTTCAAATAGGTTCCAACTCTTGTCTTGTCCATCTCCCAAGAAAGAAGCACAGAGCTCTCGGGAATGGGTCTGCAACTCTTCCACTTCTTCTGCCTATGGAGAGAAGTTTGTGGTTGTTTCTTATAACATTCTGGGTGTTGAAAATGCATCCAATCACTCAGATATGTATAATAATGTGCCACCCAGATATATGAAGTGGAAGCGTCGCAGGAGGCTTATACGAAAGGAGGTCAATAGTTACAATGCTAGCATTGTATGTTTTCAGGAGGTCGATCGTTTCGATGATTTAGCTCATAAGTTCCAGAAAGATGGGTTCGAAGGTGTGTACAAGGCACGCACTGGTGAAGTAAGTGATGGATGTGCTATATTTTGGAAACAGGACATGTTTAGCCTTTTGCATCAAGAAAGTATAGAGTTCCAAAGTTTTGGCCTTCGTCATAATGTTGCTCAATTTTGTGTTTTAAAGTTGCAAGATCAATTAGAGTCAGATGTACCTCCTCATACATCAATGAGGCCATGTACCCGAAGTAGAAGCTTAGTGATTGGGAATATACATGTCCTATTCAACCCTAGCCGTGGAGATATCAAGCTAGGGCAGGTTCGACTGTTTCTTGAAAAAGCTTACAAGCTATCGCTAGAGTGGGGATCTATCCCTGTTATAATTGCTGGGGACCTAAATAGCACTCCACAGAGTCCTATTTACCAGTTTCTAGCTTCATCTCAGGTGAGTCTGGGCCCCCTCCTCTTTATTATTACCTTTTTTCTTTCTTTTTTTCTCTGCTGTGCTAGTTTCATTTTGCAATTGTTGCTGGAGTTTTGTTGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

323

Amino Acids

37.11

Weight (kDa)

8.77

Isoelectric Point (pI)

66.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 88 - 292 4.3e-13 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 267, 803
Acc36I ACCTGC 1 cut(s) 730
AciI CCGC 1 cut(s) 116
AclWI GGATC 1 cut(s) 796
AcuI CTGAAG 1 cut(s) 75
AfaI GTAC 4 cut(s) 21, 470, 636, 664
AfiI CCNNNNNNNGG 1 cut(s) 719
AflIII ACRYGT 2 cut(s) 525, 697
AgsI TTSAA 4 cut(s) 147, 284, 709, 758
AjnI CCWGG 1 cut(s) 29
AluBI AGCT 7 cut(s) 198, 434, 678, 733, 764, 772, 857
AluI AGCT 7 cut(s) 198, 434, 678, 733, 764, 772, 857
Alw21I GWGCWC 1 cut(s) 200
AlwI GGATC 1 cut(s) 796
Ama87I CYCGRG 1 cut(s) 201
AoxI GGCC 3 cut(s) 571, 656, 877
ApaI GGGCCC 1 cut(s) 881
AspLEI GCGC 2 cut(s) 36, 90
AspS9I GGNCC 3 cut(s) 814, 877, 878
AsuHPI GGTGA 2 cut(s) 497, 880
AsuII TTCGAA 1 cut(s) 459
AsuNHI GCTAGC 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 201
AvaII GGWCC 1 cut(s) 814
BaeGI GKGCMC 1 cut(s) 881
BanII GRGCYC 2 cut(s) 200, 881
Bbv12I GWGCWC 1 cut(s) 200
BccI CCATC 4 cut(s) 20, 181, 446, 491
BceAI ACGGC 1 cut(s) 702
BciT130I CCWGG 1 cut(s) 31
BfaI CTAG 6 cut(s) 390, 714, 734, 779, 854, 930
BfuAI ACCTGC 1 cut(s) 730
Bme1390I CCNGG 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 814
BmeT110I CYCGRG 1 cut(s) 201
BmgT120I GGNCC 3 cut(s) 814, 877, 878
BmiI GGNNCC 4 cut(s) 155, 815, 879, 880
BmrFI CCNGG 1 cut(s) 31
BmsI GCATC 2 cut(s) 299, 550
BmtI GCTAGC 1 cut(s) 393
Bpu10I CCTNAGC 1 cut(s) 69
Bpu14I TTCGAA 1 cut(s) 459
BsaJI CCNNGG 2 cut(s) 29, 718
Bsc4I CCNNNNNNNGG 1 cut(s) 719
Bse1I ACTGG 2 cut(s) 487, 847
BseBI CCWGG 1 cut(s) 31
BseDI CCNNGG 2 cut(s) 29, 718
BseGI GGATG 2 cut(s) 290, 506
BseLI CCNNNNNNNGG 1 cut(s) 719
BseMII CTCAG 3 cut(s) 60, 315, 878
BseNI ACTGG 2 cut(s) 487, 847
BseRI GAGGAG 2 cut(s) 630, 875
BseSI GKGCMC 1 cut(s) 881
BseYI CCCAGC 1 cut(s) 809
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 3 cut(s) 573, 658, 879
BsiEI CGRYCG 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 201
BslFI GGGAC 2 cut(s) 12, 827
BslI CCNNNNNNNGG 1 cut(s) 719
BsmFI GGGAC 2 cut(s) 12, 827
BsnI GGCC 3 cut(s) 573, 658, 879
BsoBI CYCGRG 1 cut(s) 201
Bsp119I TTCGAA 1 cut(s) 459
Bsp120I GGGCCC 1 cut(s) 877
Bsp1286I GDGCHC 2 cut(s) 200, 881
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 3 cut(s) 415, 616, 788
BspACI CCGC 1 cut(s) 116
BspANI GGCC 3 cut(s) 573, 658, 879
BspCNI CTCAG 3 cut(s) 61, 314, 877
BspLI GGNNCC 4 cut(s) 155, 815, 879, 880
BspMI ACCTGC 1 cut(s) 730
BspOI GCTAGC 1 cut(s) 393
BspPI GGATC 1 cut(s) 796
BspT104I TTCGAA 1 cut(s) 459
BsrGI TGTACA 1 cut(s) 468
BsrI ACTGG 2 cut(s) 487, 847
BssECI CCNNGG 2 cut(s) 29, 718
BssMI GATC 3 cut(s) 415, 616, 788
Bst2UI CCWGG 1 cut(s) 31
Bst4CI ACNGT 2 cut(s) 97, 750
Bst6I CTCTTC 1 cut(s) 226
BstAPI GCANNNNNTGC 1 cut(s) 949
BstAUI TGTACA 1 cut(s) 468
BstBI TTCGAA 1 cut(s) 459
BstC8I GCNNGC 2 cut(s) 391, 478
BstDEI CTNAG 4 cut(s) 69, 301, 679, 864
BstDSI CCRYGG 1 cut(s) 718
BstF5I GGATG 2 cut(s) 290, 506
BstHHI GCGC 2 cut(s) 36, 90
BstKTI GATC 3 cut(s) 418, 619, 791
BstMBI GATC 3 cut(s) 415, 616, 788
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 2 cut(s) 31, 949
BstNI CCWGG 1 cut(s) 31
BstNSI RCATGY 2 cut(s) 529, 701
BstSCI CCNGG 1 cut(s) 29
BstSLI GKGCMC 1 cut(s) 881
BstX2I RGATCY 1 cut(s) 788
BstXI CCANNNNNNTGG 2 cut(s) 452, 569
BstYI RGATCY 1 cut(s) 788
BsuRI GGCC 3 cut(s) 573, 658, 879
BtgI CCRYGG 1 cut(s) 718
BtsCI GGATG 2 cut(s) 290, 506
BtsIMutI CAGTG 2 cut(s) 51, 480
BveI ACCTGC 1 cut(s) 730
Cac8I GCNNGC 2 cut(s) 391, 478
CfoI GCGC 2 cut(s) 36, 90
Cfr13I GGNCC 3 cut(s) 814, 877, 878
CseI GACGC 2 cut(s) 14, 335
Csp6I GTAC 4 cut(s) 20, 469, 635, 663
CviAII CATG 3 cut(s) 526, 660, 698
CviQI GTAC 4 cut(s) 20, 469, 635, 663
DdeI CTNAG 4 cut(s) 69, 301, 679, 864
DpnI GATC 3 cut(s) 417, 618, 790
DpnII GATC 3 cut(s) 415, 616, 788
DraI TTTAAA 1 cut(s) 606
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Ecl136II GAGCTC 1 cut(s) 198
Eco24I GRGCYC 2 cut(s) 200, 881
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 814
Eco53kI GAGCTC 1 cut(s) 198
Eco57I CTGAAG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 201
EcoICRI GAGCTC 1 cut(s) 198
EcoO109I RGGNCCY 2 cut(s) 814, 878
EcoRII CCWGG 1 cut(s) 29
EcoRV GATATC 1 cut(s) 727
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 2 cut(s) 200, 881
FaeI CATG 3 cut(s) 529, 663, 701
FaqI GGGAC 2 cut(s) 12, 827
FatI CATG 3 cut(s) 525, 659, 697
FokI GGATG 2 cut(s) 277, 513
FriOI GRGCYC 2 cut(s) 200, 881
FspBI CTAG 6 cut(s) 390, 714, 734, 779, 854, 930
GlaI GCGC 2 cut(s) 35, 89
GsaI CCCAGC 1 cut(s) 813
HaeIII GGCC 3 cut(s) 573, 658, 879
HgaI GACGC 2 cut(s) 14, 335
HhaI GCGC 2 cut(s) 36, 90
Hin1II CATG 3 cut(s) 529, 663, 701
Hin6I GCGC 2 cut(s) 34, 88
HinP1I GCGC 2 cut(s) 34, 88
HindIII AAGCTT 2 cut(s) 676, 762
HinfI GANTC 4 cut(s) 101, 626, 835, 871
HphI GGTGA 2 cut(s) 497, 880
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 3 cut(s) 106, 304, 631
Hpy188III TCNNGA 5 cut(s) 203, 407, 445, 545, 755
Hpy8I GTNNAC 1 cut(s) 469
Hpy99I CGWCG 1 cut(s) 351
HpyAV CCTTC 2 cut(s) 455, 584
HpyCH4III ACNGT 2 cut(s) 97, 750
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 6 cut(s) 216, 290, 541, 613, 943, 966
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 949
HpyF3I CTNAG 4 cut(s) 69, 301, 679, 864
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 3 cut(s) 529, 663, 701
HspAI GCGC 2 cut(s) 34, 88
Kzo9I GATC 3 cut(s) 415, 616, 788
LweI GCATC 2 cut(s) 299, 550
MaeI CTAG 6 cut(s) 390, 714, 734, 779, 854, 930
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 380
MalI GATC 3 cut(s) 417, 618, 790
MboI GATC 3 cut(s) 415, 616, 788
MboII GAAGA 4 cut(s) 17, 135, 213, 222
MfeI CAATTG 1 cut(s) 944
MflI RGATCY 1 cut(s) 788
MhlI GDGCHC 2 cut(s) 200, 881
MluCI AATT 4 cut(s) 593, 620, 804, 944
MlyI GAGTC 4 cut(s) 110, 635, 844, 880
MmeI TCCRAC 1 cut(s) 181
Mph1103I ATGCAT 1 cut(s) 292
MseI TTAA 1 cut(s) 605
MspR9I CCNGG 1 cut(s) 31
MunI CAATTG 1 cut(s) 944
MvaI CCWGG 1 cut(s) 31
MwoI GCNNNNNNNGC 2 cut(s) 31, 949
NdeII GATC 3 cut(s) 415, 616, 788
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 3 cut(s) 529, 663, 701
NlaIV GGNNCC 4 cut(s) 155, 815, 879, 880
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 2 cut(s) 529, 701
NspV TTCGAA 1 cut(s) 459
PciI ACATGT 2 cut(s) 525, 697
PcsI WCGNNNNNNNCGW 1 cut(s) 420
Ple19I CGATCG 1 cut(s) 418
PleI GAGTC 4 cut(s) 109, 634, 843, 879
PpsI GAGTC 4 cut(s) 109, 634, 843, 879
PpuMI RGGWCCY 1 cut(s) 814
PscI ACATGT 2 cut(s) 525, 697
PsiI TTATAA 2 cut(s) 267, 803
Psp124BI GAGCTC 1 cut(s) 200
Psp5II RGGWCCY 1 cut(s) 814
Psp6I CCWGG 1 cut(s) 29
PspFI CCCAGC 1 cut(s) 809
PspGI CCWGG 1 cut(s) 29
PspN4I GGNNCC 4 cut(s) 155, 815, 879, 880
PspOMI GGGCCC 1 cut(s) 877
PspPI GGNCC 3 cut(s) 814, 877, 878
PspPPI RGGWCCY 1 cut(s) 814
PsuI RGATCY 1 cut(s) 788
PvuI CGATCG 1 cut(s) 418
RsaI GTAC 4 cut(s) 21, 470, 636, 664
RsaNI GTAC 4 cut(s) 20, 469, 635, 663
SacI GAGCTC 1 cut(s) 200
SaqAI TTAA 1 cut(s) 605
Sau3AI GATC 3 cut(s) 415, 616, 788
Sau96I GGNCC 3 cut(s) 814, 877, 878
SchI GAGTC 4 cut(s) 110, 635, 844, 880
ScrFI CCNGG 1 cut(s) 31
SduI GDGCHC 2 cut(s) 200, 881
SfaNI GCATC 2 cut(s) 299, 550
SfuI TTCGAA 1 cut(s) 459
SinI GGWCC 1 cut(s) 814
Sse9I AATT 4 cut(s) 593, 620, 804, 944
SsiI CCGC 1 cut(s) 116
SspMI CTAG 6 cut(s) 390, 714, 734, 779, 854, 930
SstI GAGCTC 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 29
TaaI ACNGT 2 cut(s) 97, 750
TaiI ACGT 1 cut(s) 21
TaqI TCGA 4 cut(s) 414, 423, 459, 745
TasI AATT 4 cut(s) 593, 620, 804, 944
TatI WGTACW 1 cut(s) 468
Tru1I TTAA 1 cut(s) 605
Tru9I TTAA 1 cut(s) 605
TscAI CASTG 2 cut(s) 58, 487
TspDTI ATGAA 3 cut(s) 350, 849, 925
TspRI CASTG 2 cut(s) 58, 487
VpaK11BI GGWCC 1 cut(s) 814
XceI RCATGY 2 cut(s) 529, 701
XspI CTAG 6 cut(s) 390, 714, 734, 779, 854, 930
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.