FvH4_5g12610

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0017665
Physical Location & Seq
Reverse (-)
7117885 .. 7120933
3049 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12610.t1

Sequence Viewer

Length: 834 bp
ATGGCGGCAGAGAAGTGGCTTATGGTGGTCGTAGACGGAACAGCAGCTATGGGGCCTTACTGGAAATCCATTCTCTCAGATTACCTCGAAAAGATCATCAGGAACATATGTGGCCAATCGTCAGCTGACCAGGATGCTTCAGCTGGTGCAGCAGTATACCTGGGGCTGGTTCGGTATAACTCTCACGGACTTGGTAATTCTGGTTGCTTAATGCAAGCAAGCCCTTGGACGAGAAATGTGGAAGGGTTTCTTGGATGGCTCTCGGCTTTACATTTTTCTGGTGGGGGTGGTTCTCATGATGCTGCTATTGCTGAGGGCTTGGCAGAAGCACTTGTGATGTTTCCGAAGCCTACTCCGACTGCAGACGGAACTATATCTGGAACTGAAACTAGAACTGGAAGTGAAACTGAAACTGAAACTGATAGAGTTCAAGACCATAATGCCAGAGAAAGGCATTGTATTCTTGTGGCTGCAAGCAACCCTATGTTGATTCCAACGTACGTTCAGGTACCGTTGATAAGTAATGGCCAATTTTCTCCTGGTACAAAAACTCGAATGACTCTTGCTGATGCTCAAGATGTGGCCAAATTATATGCTGAGTGCTCTGTATCTCTTTCTGTCATCTCGCCGAAACAGTTTCCAAAACTCAGAGAGATATATAATGCGGGAAGAAATGATCCTGAATCTGAAGGAAAAGTCTCTTCCTTCAGAAATATTAAGTACCCACATTTTCTTGTACTGTTATCAAACAAGTTCCCAGAGGTTCATGCTGCCCTCGATCACTCTCAGACTGTGATCAAACCTCCATACAATGTCTTTCTCAGCTCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007602 GO:0008150 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009639 GO:0009653 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010017 GO:0010026 GO:0010033 GO:0010090 GO:0010091 GO:0010114 GO:0010218 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010927 GO:0016043 GO:0016592 GO:0019219 GO:0019222 GO:0022607 GO:0023052 GO:0030030 GO:0030031 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032502 GO:0032870 GO:0032989 GO:0032991 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048646 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0071214 GO:0071229 GO:0071310 GO:0071395 GO:0071478 GO:0071482 GO:0071489 GO:0071495 GO:0071840 GO:0080090 GO:0080134 GO:0090558 GO:0090626 GO:0097159 GO:0098542 GO:0104004 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1905499 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

278

Amino Acids

29.94

Weight (kDa)

6.06

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 7 - 115 6.2e-22 Mediator complex subunit 25 von Willebrand factor type A
Med25_VWA PF11265 140 - 249 1.6e-12 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017665)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12601 FvH4_5g12610
rosa_chinensis RchiOBHm_Chr7g0184721
rosa_laevigata RLG00000004956
rosa_multiflora Rmu_ssc0000289.1_g000020
rosa_roxburghii Rroxscaffold_3G00269400
rosa_rugosa Rorug06G0464700
rosa_samantha Rh7AG078200 Rh7BG069100 Rh7CG069900 Rh7DG069300
rosa_wichuraiana Rw7G005720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 508
AccB1I GGYRCC 1 cut(s) 508
AccI GTMKAC 2 cut(s) 33, 156
AciI CCGC 2 cut(s) 5, 665
AclWI GGATC 1 cut(s) 671
AcoI YGGCCR 3 cut(s) 112, 526, 582
AcuI CTGAAG 3 cut(s) 123, 691, 708
AfaI GTAC 5 cut(s) 500, 510, 544, 722, 738
AfiI CCNNNNNNNGG 2 cut(s) 166, 450
AgsI TTSAA 1 cut(s) 431
AjnI CCWGG 3 cut(s) 129, 159, 538
AjuI GAANNNNNNNTTGG 2 cut(s) 234, 266
AluBI AGCT 4 cut(s) 47, 125, 143, 825
AluI AGCT 4 cut(s) 47, 125, 143, 825
Alw21I GWGCWC 1 cut(s) 605
Alw26I GTCTC 1 cut(s) 703
AlwI GGATC 1 cut(s) 671
AoxI GGCC 4 cut(s) 53, 112, 526, 582
ApeKI GCWGC 5 cut(s) 44, 149, 302, 470, 770
Asp700I GAANNNNTTC 1 cut(s) 246
Asp718I GGTACC 1 cut(s) 508
AspS9I GGNCC 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 819
BalI TGGCCA 3 cut(s) 114, 528, 584
BanI GGYRCC 1 cut(s) 508
Bbv12I GWGCWC 1 cut(s) 605
BbvCI CCTCAGC 1 cut(s) 312
BbvI GCAGC 5 cut(s) 56, 161, 289, 457, 757
BccI CCATC 1 cut(s) 249
BciT130I CCWGG 3 cut(s) 131, 161, 540
BclI TGATCA 1 cut(s) 795
BcoDI GTCTC 1 cut(s) 703
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 1 cut(s) 360
BisI GCNGC 6 cut(s) 6, 45, 150, 303, 471, 771
BlsI GCNGC 6 cut(s) 7, 46, 151, 304, 472, 772
Bme1390I CCNGG 3 cut(s) 131, 161, 540
BmgT120I GGNCC 1 cut(s) 53
BmiI GGNNCC 2 cut(s) 54, 510
BmrFI CCNGG 3 cut(s) 131, 161, 540
BmsI GCATC 3 cut(s) 124, 289, 559
Bpu10I CCTNAGC 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 558
BsaJI CCNNGG 2 cut(s) 160, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 450
Bse1I ACTGG 2 cut(s) 65, 400
BseBI CCWGG 3 cut(s) 131, 161, 540
BseDI CCNNGG 2 cut(s) 160, 224
BseGI GGATG 2 cut(s) 139, 260
BseLI CCNNNNNNNGG 2 cut(s) 166, 450
BseMII CTCAG 5 cut(s) 90, 303, 588, 661, 800
BseNI ACTGG 2 cut(s) 65, 400
BseXI GCAGC 5 cut(s) 56, 161, 289, 457, 757
BsgI GTGCAG 1 cut(s) 168
BshFI GGCC 4 cut(s) 55, 114, 528, 584
BshNI GGYRCC 1 cut(s) 508
BsiHKAI GWGCWC 1 cut(s) 605
BsiWI CGTACG 1 cut(s) 498
BslI CCNNNNNNNGG 2 cut(s) 166, 450
BsmAI GTCTC 1 cut(s) 703
BsnI GGCC 4 cut(s) 55, 114, 528, 584
Bsp1286I GDGCHC 1 cut(s) 605
Bsp143I GATC 4 cut(s) 93, 676, 778, 795
BspACI CCGC 2 cut(s) 5, 665
BspANI GGCC 4 cut(s) 55, 114, 528, 584
BspCNI CTCAG 6 cut(s) 89, 304, 589, 660, 799, 834
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 54, 510
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 1 cut(s) 671
BspT107I GGYRCC 1 cut(s) 508
BsrI ACTGG 2 cut(s) 65, 400
BssECI CCNNGG 2 cut(s) 160, 224
BssMI GATC 4 cut(s) 93, 676, 778, 795
BssNAI GTATAC 1 cut(s) 157
BssT1I CCWWGG 1 cut(s) 224
Bst1107I GTATAC 1 cut(s) 157
Bst2UI CCWGG 3 cut(s) 131, 161, 540
Bst4CI ACNGT 4 cut(s) 513, 636, 741, 793
Bst6I CTCTTC 1 cut(s) 706
BstC8I GCNNGC 3 cut(s) 216, 220, 475
BstDEI CTNAG 6 cut(s) 76, 312, 597, 647, 786, 821
BstF5I GGATG 2 cut(s) 139, 260
BstKTI GATC 4 cut(s) 96, 679, 781, 798
BstMAI GTCTC 1 cut(s) 703
BstMBI GATC 4 cut(s) 93, 676, 778, 795
BstMWI GCNNNNNNNGC 2 cut(s) 149, 308
BstNI CCWGG 3 cut(s) 131, 161, 540
BstSCI CCNGG 3 cut(s) 129, 159, 538
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 5 cut(s) 56, 161, 289, 457, 757
BstZ17I GTATAC 1 cut(s) 157
BsuRI GGCC 4 cut(s) 55, 114, 528, 584
BtsCI GGATG 2 cut(s) 139, 260
Cac8I GCNNGC 3 cut(s) 216, 220, 475
CciI TCATGA 1 cut(s) 295
Cfr13I GGNCC 1 cut(s) 53
Csp6I GTAC 5 cut(s) 499, 509, 543, 721, 737
CviAII CATG 3 cut(s) 296, 767, 831
CviQI GTAC 5 cut(s) 499, 509, 543, 721, 737
DdeI CTNAG 6 cut(s) 76, 312, 597, 647, 786, 821
DpnI GATC 4 cut(s) 95, 678, 780, 797
DpnII GATC 4 cut(s) 93, 676, 778, 795
EaeI YGGCCR 3 cut(s) 112, 526, 582
Eam1104I CTCTTC 1 cut(s) 706
EarI CTCTTC 1 cut(s) 706
Eco130I CCWWGG 1 cut(s) 224
Eco57I CTGAAG 3 cut(s) 123, 691, 708
EcoO109I RGGNCCY 1 cut(s) 53
EcoRII CCWGG 3 cut(s) 129, 159, 538
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 3 cut(s) 299, 770, 834
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FatI CATG 3 cut(s) 295, 766, 830
FauI CCCGC 1 cut(s) 658
FauNDI CATATG 1 cut(s) 107
FbaI TGATCA 1 cut(s) 795
FblI GTMKAC 2 cut(s) 33, 156
Fnu4HI GCNGC 6 cut(s) 6, 45, 150, 303, 471, 771
FokI GGATG 2 cut(s) 146, 267
Fsp4HI GCNGC 6 cut(s) 6, 45, 150, 303, 471, 771
FspBI CTAG 1 cut(s) 390
GluI GCNGC 6 cut(s) 6, 45, 150, 303, 471, 771
HaeIII GGCC 4 cut(s) 55, 114, 528, 584
Hin1II CATG 3 cut(s) 299, 770, 834
HinfI GANTC 3 cut(s) 490, 559, 683
HphI GGTGA 1 cut(s) 819
Hpy166II GTNNAC 2 cut(s) 34, 157
Hpy188I TCNGA 7 cut(s) 79, 345, 357, 650, 688, 710, 789
Hpy188III TCNNGA 6 cut(s) 100, 296, 378, 431, 575, 680
Hpy8I GTNNAC 2 cut(s) 34, 157
HpyAV CCTTC 3 cut(s) 236, 683, 715
HpyCH4III ACNGT 4 cut(s) 513, 636, 741, 793
HpyCH4IV ACGT 2 cut(s) 497, 501
HpyCH4V TGCA 4 cut(s) 149, 214, 362, 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 308
HpyF3I CTNAG 6 cut(s) 76, 312, 597, 647, 786, 821
HpySE526I ACGT 2 cut(s) 497, 501
Hsp92II CATG 3 cut(s) 299, 770, 834
KpnI GGTACC 1 cut(s) 512
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 4 cut(s) 93, 676, 778, 795
Lsp1109I GCAGC 5 cut(s) 56, 161, 289, 457, 757
LweI GCATC 3 cut(s) 124, 289, 559
MaeI CTAG 1 cut(s) 390
MaeII ACGT 2 cut(s) 497, 501
MalI GATC 4 cut(s) 95, 678, 780, 797
MboI GATC 4 cut(s) 93, 676, 778, 795
MboII GAAGA 2 cut(s) 681, 693
MhlI GDGCHC 1 cut(s) 605
MlsI TGGCCA 3 cut(s) 114, 528, 584
MluCI AATT 3 cut(s) 196, 530, 587
MluNI TGGCCA 3 cut(s) 114, 528, 584
MlyI GAGTC 1 cut(s) 553
MmeI TCCRAC 2 cut(s) 380, 518
MnlI CCTC 5 cut(s) 95, 307, 754, 785, 813
Mox20I TGGCCA 3 cut(s) 114, 528, 584
MroXI GAANNNNTTC 1 cut(s) 246
MscI TGGCCA 3 cut(s) 114, 528, 584
MseI TTAA 2 cut(s) 209, 717
Msp20I TGGCCA 3 cut(s) 114, 528, 584
MspA1I CMGCKG 2 cut(s) 125, 143
MspR9I CCNGG 3 cut(s) 131, 161, 540
MvaI CCWGG 3 cut(s) 131, 161, 540
MwoI GCNNNNNNNGC 2 cut(s) 149, 308
NdeI CATATG 1 cut(s) 107
NdeII GATC 4 cut(s) 93, 676, 778, 795
NlaIII CATG 3 cut(s) 299, 770, 834
NlaIV GGNNCC 2 cut(s) 54, 510
NmeAIII GCCGAG 1 cut(s) 242
PagI TCATGA 1 cut(s) 295
PdmI GAANNNNTTC 1 cut(s) 246
PfeI GAWTC 2 cut(s) 490, 683
Pfl23II CGTACG 1 cut(s) 498
PkrI GCNGC 6 cut(s) 7, 46, 151, 304, 472, 772
PleI GAGTC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 553
Psp6I CCWGG 3 cut(s) 129, 159, 538
PspGI CCWGG 3 cut(s) 129, 159, 538
PspLI CGTACG 1 cut(s) 498
PspN4I GGNNCC 2 cut(s) 54, 510
PspPI GGNCC 1 cut(s) 53
PstI CTGCAG 1 cut(s) 364
PvuII CAGCTG 2 cut(s) 125, 143
RsaI GTAC 5 cut(s) 500, 510, 544, 722, 738
RsaNI GTAC 5 cut(s) 499, 509, 543, 721, 737
SaqAI TTAA 2 cut(s) 209, 717
SatI GCNGC 6 cut(s) 6, 45, 150, 303, 471, 771
Sau3AI GATC 4 cut(s) 93, 676, 778, 795
Sau96I GGNCC 1 cut(s) 53
SchI GAGTC 1 cut(s) 553
ScrFI CCNGG 3 cut(s) 131, 161, 540
SduI GDGCHC 1 cut(s) 605
SfaNI GCATC 3 cut(s) 124, 289, 559
SfcI CTRYAG 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 573
SmoI CTYRAG 1 cut(s) 573
Sse9I AATT 3 cut(s) 196, 530, 587
SsiI CCGC 2 cut(s) 5, 665
SspI AATATT 1 cut(s) 715
SspMI CTAG 1 cut(s) 390
StyD4I CCNGG 3 cut(s) 129, 159, 538
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 4 cut(s) 513, 636, 741, 793
TaiI ACGT 2 cut(s) 500, 504
TaqI TCGA 3 cut(s) 87, 553, 777
TasI AATT 3 cut(s) 196, 530, 587
TatI WGTACW 1 cut(s) 736
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 490, 683
Tru1I TTAA 2 cut(s) 209, 717
Tru9I TTAA 2 cut(s) 209, 717
TseI GCWGC 5 cut(s) 44, 149, 302, 470, 770
TspDTI ATGAA 1 cut(s) 755
TspGWI ACGGA 3 cut(s) 51, 201, 381
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XmiI GTMKAC 2 cut(s) 33, 156
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.