RLG00000004956

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017665
Physical Location & Seq
Reverse (-)
63962563 .. 63971124
8562 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004956

Sequence Viewer

Length: 1851 bp
ATGGGGCCTTACTGGAAATCCATTCTCTCAGATTACCTCGAAAAGATCATCTGGAACATATGTGGCCAACCGTCAGCTAACCAGGATGCTTCAGCTGGTGCAGCAGTATACCTGGGCCTGGTTCAGTGTAATTCTCACGGACTTGGTAATACTGGTTCCTTAACGCGACCAAGTCCTTGGACTAGAAATGTGGATGGGTTTCTTGAAGGGCTCTCGGCTTTACATTTTTCTGGTGGTGGTGGTCCTAGTGAAGCCGCCATTGCTGGGGGGCTGGCAGAAGCACTTGTAATGTTTCCGGAGCCTACTCCCACTGGAACTGGAACTGAAACTGAAACTGATCAAGTTCAAGACCATAATGCCAGAGAAAGGCATTGTATTCTTGTGGCTGCAAGCAACCCTCTGTTGTTTCGAACCGCCGTTCAGATACCGATAATAAGTAATGGCCAATTTTCTTCTGGTACACAAACTCGATGGACTCTTGCTGATGCTCAAGATGTTGCCAAATTATATGCTGAGGTAGATATCAAAGAAGATATGCAAATAATACACACAAACATCCTTTCATTTTCAGTTCCTAGAACACCTTCCTTCAGCGATGCGAACAATCCTGACGTACTTCTTCTTTTGTCGAAGGACTTCTTAGAGGCTCATGCTGCCCTTATTCAGTATGCAGAAGTGAACAGACTGTCAGCTCAGGAGATAAGTCAGATAACAGACTCTGAGCTTGAATATATTGCTTTAGTACATTTCGGAGACAACAGTGAGTTTGATTATATAGGAGATATGTTGGATCCACCCGAGACAGATAAGCAAGATCATAGTGCAGAGAACATTCTCAATGAAACCGAAGAAATGCTTCTCGCAGCAACACAAGACAGTTTTTCACAAGCACCTTCACCCAGCTGTTTCAATATTAGTGAGACATTGTCCAGTAGCCTTATTCAGTACCCCGAAGTACAGGCAAGGGGATCTGAAGCCGGAGAAAGTACTGCTAGTACTACGCAAGCAGAACTCCACTCTCCACAGCAGGCGCAACAAATACTTGCAACTCGTACCATAAGTGGAGTACACAGATCGCAAGGATCTTGCAGGGATCCAAGTAGTCCACAAACCACAACTTCTTTACACACTTTTGCAGCAACTTCTGGCAACTCAAGGGGTCCACGAGCATCTTCACGTACTCAAAGATCTCAAATAAGTATGGATCCTAATTTCAAACCAAGAAGAAGTCCAAGAATTGCATCAAGAGCTACTGTGGACCAAGGAGGACATGAAGGTGCTTTTGCTCGGTTTGGAGAACCATCTGGTCCACACACATCAGGTACGATCTTCGCCAAGAATCAAGAACGGCTTGTTCTAGTCAGTAAGGCAAAGGCGCACAAGATGAAGTCTGCACCAGAGAGCCTTACTACTGGTTGGCCACGACTGCTGAAGATGGGTAATGTTATTCCAGAAGCTACTTTTGGCAGGTCTTATGAAAGACAAGCCCATGATTTGATCTTTGAGATTACGGGTGACGAGAAAATATATGACCTACTAAATAAGAATGAGCTGTGTATAAGGTTCATCCTGCCTACTCAGTCATTGATAATTATCCCAGCTGAACACAACCACTTCACCGGGAGAGTGTATGCAGGGGAAGGACCTCTAATGGAGCAACATCTGGTTGAACAATACAATCAACAAAGGGATCAGCAGCACCAATATGCCATAACTGGGGAAGGACCTCCAGTGGAGCAACATATGGTTGAACAATACAATCAAAGAAAGCATCAGCACCAGCAATATGCCACAACTGGTATAAAACTGCGCGGTGAGCTTTCAAGTAGAGGGACGACCAACAGATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

617

Amino Acids

67.51

Weight (kDa)

5.5

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 1 - 175 8.8e-16 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017665)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12601 FvH4_5g12610
rosa_chinensis RchiOBHm_Chr7g0184721
rosa_laevigata RLG00000004956
rosa_multiflora Rmu_ssc0000289.1_g000020
rosa_roxburghii Rroxscaffold_3G00269400
rosa_rugosa Rorug06G0464700
rosa_samantha Rh7AG078200 Rh7BG069100 Rh7CG069900 Rh7DG069300
rosa_wichuraiana Rw7G005720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1458
AccI GTMKAC 1 cut(s) 108
AccII CGCG 2 cut(s) 166, 1812
AccIII TCCGGA 1 cut(s) 295
AciI CCGC 3 cut(s) 255, 414, 1812
AcoI YGGCCR 3 cut(s) 64, 442, 1418
AcuI CTGAAG 4 cut(s) 75, 574, 993, 1451
AfiI CCNNNNNNNGG 4 cut(s) 118, 264, 366, 1716
AgsI TTSAA 8 cut(s) 206, 347, 728, 910, 1216, 1670, 1751, 1824
AjnI CCWGG 3 cut(s) 81, 111, 117
AjuI GAANNNNNNNTTGG 2 cut(s) 1446, 1478
Alw26I GTCTC 3 cut(s) 747, 794, 914
AlwNI CAGNNNCTG 1 cut(s) 719
Ama87I CYCGRG 1 cut(s) 797
Aor13HI TCCGGA 1 cut(s) 295
AoxI GGCC 5 cut(s) 5, 64, 115, 442, 1418
ApeKI GCWGC 6 cut(s) 101, 386, 653, 863, 1136, 1696
Asp700I GAANNNNTTC 3 cut(s) 583, 635, 855
AspLEI GCGC 3 cut(s) 1033, 1378, 1812
AspS9I GGNCC 8 cut(s) 5, 115, 242, 1160, 1258, 1307, 1643, 1724
AsuC2I CCSGG 1 cut(s) 1621
AsuHPI GGTGA 4 cut(s) 888, 1526, 1609, 1826
AsuII TTCGAA 1 cut(s) 409
AvaI CYCGRG 1 cut(s) 797
AvaII GGWCC 6 cut(s) 242, 1160, 1258, 1307, 1643, 1724
BalI TGGCCA 3 cut(s) 66, 444, 1420
BamHI GGATCC 3 cut(s) 790, 1093, 1204
BanII GRGCYC 1 cut(s) 213
BauI CACGAG 1 cut(s) 1164
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 6 cut(s) 113, 373, 640, 875, 1148, 1708
BccI CCATC 4 cut(s) 188, 465, 1309, 1429
BceAI ACGGC 2 cut(s) 401, 1364
BciT130I CCWGG 3 cut(s) 83, 113, 119
BclI TGATCA 1 cut(s) 337
BcnI CCSGG 1 cut(s) 1621
BcoDI GTCTC 3 cut(s) 747, 794, 914
BfaI CTAG 6 cut(s) 183, 246, 576, 993, 1358, 1849
BfuAI ACCTGC 1 cut(s) 1458
BglII AGATCT 1 cut(s) 1187
BisI GCNGC 7 cut(s) 102, 255, 387, 654, 864, 1137, 1697
BlsI GCNGC 7 cut(s) 103, 256, 388, 655, 865, 1138, 1698
BmcAI AGTACT 2 cut(s) 988, 997
Bme1390I CCNGG 4 cut(s) 83, 113, 119, 1621
Bme18I GGWCC 6 cut(s) 242, 1160, 1258, 1307, 1643, 1724
BmeT110I CYCGRG 1 cut(s) 797
BmgT120I GGNCC 8 cut(s) 5, 115, 242, 1160, 1258, 1307, 1643, 1724
BmiI GGNNCC 7 cut(s) 6, 157, 300, 792, 1095, 1161, 1206
BmrFI CCNGG 4 cut(s) 83, 113, 119, 1621
BmrI ACTGGG 1 cut(s) 1725
BmsI GCATC 6 cut(s) 76, 475, 586, 1178, 1250, 1780
BmuI ACTGGG 1 cut(s) 1725
BpmI CTGGAG 1 cut(s) 1713
Bpu10I CCTNAGC 2 cut(s) 513, 693
Bpu14I TTCGAA 1 cut(s) 409
BpuEI CTTGAG 2 cut(s) 474, 1138
BpuMI CCSGG 1 cut(s) 1621
BsaAI YACGTR 1 cut(s) 1178
BsaBI GATNNNNATC 1 cut(s) 1592
BsaJI CCNNGG 3 cut(s) 112, 176, 1261
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 264, 366, 1716
Bse1I ACTGG 9 cut(s) 17, 157, 316, 322, 930, 1417, 1720, 1730, 1801
Bse3DI GCAATG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 1592
BseAI TCCGGA 1 cut(s) 295
BseBI CCWGG 3 cut(s) 83, 113, 119
BseDI CCNNGG 3 cut(s) 112, 176, 1261
BseGI GGATG 4 cut(s) 91, 199, 555, 1566
BseJI GATNNNNATC 1 cut(s) 1592
BseLI CCNNNNNNNGG 4 cut(s) 118, 264, 366, 1716
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 5 cut(s) 42, 504, 707, 711, 1592
BseNI ACTGG 9 cut(s) 17, 157, 316, 322, 930, 1417, 1720, 1730, 1801
BseXI GCAGC 6 cut(s) 113, 373, 640, 875, 1148, 1708
BseYI CCCAGC 3 cut(s) 263, 899, 1597
BsgI GTGCAG 3 cut(s) 120, 843, 1377
Bsh1236I CGCG 2 cut(s) 166, 1812
BshFI GGCC 5 cut(s) 7, 66, 117, 444, 1420
BsiHKCI CYCGRG 1 cut(s) 797
BsiSI CCGG 3 cut(s) 296, 978, 1620
BslFI GGGAC 1 cut(s) 1846
BslI CCNNNNNNNGG 4 cut(s) 118, 264, 366, 1716
BsmAI GTCTC 3 cut(s) 747, 794, 914
BsmFI GGGAC 1 cut(s) 1846
BsnI GGCC 5 cut(s) 7, 66, 117, 444, 1420
BsoBI CYCGRG 1 cut(s) 797
Bsp119I TTCGAA 1 cut(s) 409
Bsp1286I GDGCHC 1 cut(s) 213
Bsp13I TCCGGA 1 cut(s) 295
BspACI CCGC 3 cut(s) 255, 414, 1812
BspANI GGCC 5 cut(s) 7, 66, 117, 444, 1420
BspCNI CTCAG 5 cut(s) 41, 505, 706, 712, 1591
BspEI TCCGGA 1 cut(s) 295
BspFNI CGCG 2 cut(s) 166, 1812
BspLI GGNNCC 7 cut(s) 6, 157, 300, 792, 1095, 1161, 1206
BspMI ACCTGC 1 cut(s) 1458
BspT104I TTCGAA 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 9 cut(s) 17, 157, 316, 322, 930, 1417, 1720, 1730, 1801
BssECI CCNNGG 3 cut(s) 112, 176, 1261
BssNAI GTATAC 1 cut(s) 109
BssSI CACGAG 1 cut(s) 1164
BssT1I CCWWGG 2 cut(s) 176, 1261
Bst1107I GTATAC 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 1164
Bst2UI CCWGG 3 cut(s) 83, 113, 119
Bst4CI ACNGT 5 cut(s) 72, 687, 761, 878, 1255
BstBAI YACGTR 1 cut(s) 1178
BstBI TTCGAA 1 cut(s) 409
BstC8I GCNNGC 4 cut(s) 273, 391, 1005, 1029
BstDEI CTNAG 6 cut(s) 28, 513, 640, 693, 720, 1578
BstF5I GGATG 4 cut(s) 91, 199, 555, 1566
BstFNI CGCG 2 cut(s) 166, 1812
BstHHI GCGC 3 cut(s) 1033, 1378, 1812
BstMAI GTCTC 3 cut(s) 747, 794, 914
BstMWI GCNNNNNNNGC 6 cut(s) 101, 260, 653, 1247, 1426, 1816
BstNI CCWGG 3 cut(s) 83, 113, 119
BstSCI CCNGG 4 cut(s) 81, 111, 117, 1619
BstUI CGCG 2 cut(s) 166, 1812
BstV1I GCAGC 6 cut(s) 113, 373, 640, 875, 1148, 1708
BstX2I RGATCY 7 cut(s) 790, 968, 1082, 1093, 1187, 1204, 1844
BstXI CCANNNNNNTGG 1 cut(s) 177
BstYI RGATCY 7 cut(s) 790, 968, 1082, 1093, 1187, 1204, 1844
BstZ17I GTATAC 1 cut(s) 109
BsuRI GGCC 5 cut(s) 7, 66, 117, 444, 1420
BtgZI GCGATG 1 cut(s) 609
BtsCI GGATG 4 cut(s) 91, 199, 555, 1566
BtsIMutI CAGTG 4 cut(s) 131, 309, 766, 1737
BveI ACCTGC 1 cut(s) 1458
Cac8I GCNNGC 4 cut(s) 273, 391, 1005, 1029
CaiI CAGNNNCTG 1 cut(s) 719
CfoI GCGC 3 cut(s) 1033, 1378, 1812
Cfr13I GGNCC 8 cut(s) 5, 115, 242, 1160, 1258, 1307, 1643, 1724
CviAII CATG 3 cut(s) 650, 1271, 1490
DdeI CTNAG 6 cut(s) 28, 513, 640, 693, 720, 1578
EaeI YGGCCR 3 cut(s) 64, 442, 1418
Eco130I CCWWGG 2 cut(s) 176, 1261
Eco24I GRGCYC 1 cut(s) 213
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 6 cut(s) 242, 1160, 1258, 1307, 1643, 1724
Eco57I CTGAAG 4 cut(s) 75, 574, 993, 1451
Eco88I CYCGRG 1 cut(s) 797
EcoO109I RGGNCCY 3 cut(s) 5, 1643, 1724
EcoRII CCWGG 3 cut(s) 81, 111, 117
EcoRV GATATC 1 cut(s) 523
EcoT14I CCWWGG 2 cut(s) 176, 1261
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 2 cut(s) 176, 1261
FaeI CATG 3 cut(s) 653, 1274, 1493
FalI AAGNNNNNCTT 6 cut(s) 623, 655, 840, 872, 1335, 1367
FaqI GGGAC 1 cut(s) 1846
FatI CATG 3 cut(s) 649, 1270, 1489
FauNDI CATATG 2 cut(s) 59, 1743
FbaI TGATCA 1 cut(s) 337
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 7 cut(s) 102, 255, 387, 654, 864, 1137, 1697
FokI GGATG 4 cut(s) 98, 206, 542, 1553
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 7 cut(s) 102, 255, 387, 654, 864, 1137, 1697
FspBI CTAG 6 cut(s) 183, 246, 576, 993, 1358, 1849
GlaI GCGC 3 cut(s) 1032, 1377, 1811
GluI GCNGC 7 cut(s) 102, 255, 387, 654, 864, 1137, 1697
GsaI CCCAGC 3 cut(s) 267, 903, 1601
GsuI CTGGAG 1 cut(s) 1713
HaeIII GGCC 5 cut(s) 7, 66, 117, 444, 1420
HapII CCGG 3 cut(s) 296, 978, 1620
HhaI GCGC 3 cut(s) 1033, 1378, 1812
Hin1II CATG 3 cut(s) 653, 1274, 1493
Hin6I GCGC 3 cut(s) 1031, 1376, 1810
HinP1I GCGC 3 cut(s) 1031, 1376, 1810
HinfI GANTC 3 cut(s) 475, 716, 1339
HpaII CCGG 3 cut(s) 296, 978, 1620
HphI GGTGA 4 cut(s) 888, 1526, 1609, 1826
Hpy166II GTNNAC 8 cut(s) 109, 461, 679, 1069, 1106, 1163, 1258, 1310
Hpy188I TCNGA 6 cut(s) 31, 423, 708, 721, 752, 973
Hpy8I GTNNAC 8 cut(s) 109, 461, 679, 1069, 1106, 1163, 1258, 1310
HpyAV CCTTC 8 cut(s) 200, 594, 598, 625, 903, 1268, 1634, 1715
HpyCH4III ACNGT 5 cut(s) 72, 687, 761, 878, 1255
HpyCH4IV ACGT 2 cut(s) 612, 1177
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 260, 653, 1247, 1426, 1816
HpyF3I CTNAG 6 cut(s) 28, 513, 640, 693, 720, 1578
HpySE526I ACGT 2 cut(s) 612, 1177
Hsp92II CATG 3 cut(s) 653, 1274, 1493
HspAI GCGC 3 cut(s) 1031, 1376, 1810
Kpn2I TCCGGA 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 337
LmnI GCTCC 3 cut(s) 298, 1654, 1735
Lsp1109I GCAGC 6 cut(s) 113, 373, 640, 875, 1148, 1708
LweI GCATC 6 cut(s) 76, 475, 586, 1178, 1250, 1780
MaeI CTAG 6 cut(s) 183, 246, 576, 993, 1358, 1849
MaeII ACGT 2 cut(s) 612, 1177
MaeIII GTNAC 1 cut(s) 1514
MboII GAAGA 8 cut(s) 444, 542, 611, 860, 1164, 1236, 1321, 1444
MflI RGATCY 7 cut(s) 790, 968, 1082, 1093, 1187, 1204, 1844
MhlI GDGCHC 1 cut(s) 213
MlsI TGGCCA 3 cut(s) 66, 444, 1420
MluCI AATT 6 cut(s) 130, 446, 503, 1210, 1236, 1590
MluNI TGGCCA 3 cut(s) 66, 444, 1420
MlyI GAGTC 2 cut(s) 469, 710
MmeI TCCRAC 1 cut(s) 768
MnlI CCTC 8 cut(s) 47, 408, 508, 637, 1259, 1656, 1737, 1823
Mox20I TGGCCA 3 cut(s) 66, 444, 1420
MroI TCCGGA 1 cut(s) 295
MroXI GAANNNNTTC 3 cut(s) 583, 635, 855
MscI TGGCCA 3 cut(s) 66, 444, 1420
MseI TTAA 1 cut(s) 161
MslI CAYNNNNRTG 2 cut(s) 1275, 1704
Msp20I TGGCCA 3 cut(s) 66, 444, 1420
MspA1I CMGCKG 3 cut(s) 95, 903, 1601
MspI CCGG 3 cut(s) 296, 978, 1620
MspR9I CCNGG 4 cut(s) 83, 113, 119, 1621
MvaI CCWGG 3 cut(s) 83, 113, 119
MvnI CGCG 2 cut(s) 166, 1812
MwoI GCNNNNNNNGC 6 cut(s) 101, 260, 653, 1247, 1426, 1816
NciI CCSGG 1 cut(s) 1621
NdeI CATATG 2 cut(s) 59, 1743
NlaIII CATG 3 cut(s) 653, 1274, 1493
NlaIV GGNNCC 7 cut(s) 6, 157, 300, 792, 1095, 1161, 1206
NmeAIII GCCGAG 1 cut(s) 194
NmuCI GTSAC 1 cut(s) 1514
NspV TTCGAA 1 cut(s) 409
PdmI GAANNNNTTC 3 cut(s) 583, 635, 855
PfeI GAWTC 1 cut(s) 1339
PflFI GACNNNGTC 2 cut(s) 171, 925
PkrI GCNGC 7 cut(s) 103, 256, 388, 655, 865, 1138, 1698
PleI GAGTC 2 cut(s) 469, 710
PpsI GAGTC 2 cut(s) 469, 710
Ppu21I YACGTR 1 cut(s) 1178
PpuMI RGGWCCY 2 cut(s) 1643, 1724
Psp5II RGGWCCY 2 cut(s) 1643, 1724
Psp6I CCWGG 3 cut(s) 81, 111, 117
PspFI CCCAGC 3 cut(s) 263, 899, 1597
PspGI CCWGG 3 cut(s) 81, 111, 117
PspN4I GGNNCC 7 cut(s) 6, 157, 300, 792, 1095, 1161, 1206
PspPI GGNCC 8 cut(s) 5, 115, 242, 1160, 1258, 1307, 1643, 1724
PspPPI RGGWCCY 2 cut(s) 1643, 1724
PsrI GAACNNNNNNTAC 2 cut(s) 139, 171
PstNI CAGNNNCTG 1 cut(s) 719
PsuI RGATCY 7 cut(s) 790, 968, 1082, 1093, 1187, 1204, 1844
PsyI GACNNNGTC 2 cut(s) 171, 925
PvuII CAGCTG 3 cut(s) 95, 903, 1601
RseI CAYNNNNRTG 2 cut(s) 1275, 1704
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 7 cut(s) 102, 255, 387, 654, 864, 1137, 1697
Sau96I GGNCC 8 cut(s) 5, 115, 242, 1160, 1258, 1307, 1643, 1724
ScaI AGTACT 2 cut(s) 988, 997
SchI GAGTC 2 cut(s) 469, 710
ScrFI CCNGG 4 cut(s) 83, 113, 119, 1621
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 6 cut(s) 76, 475, 586, 1178, 1250, 1780
SfuI TTCGAA 1 cut(s) 409
SinI GGWCC 6 cut(s) 242, 1160, 1258, 1307, 1643, 1724
SmiMI CAYNNNNRTG 2 cut(s) 1275, 1704
SmlI CTYRAG 2 cut(s) 489, 1153
SmoI CTYRAG 2 cut(s) 489, 1153
Sse9I AATT 6 cut(s) 130, 446, 503, 1210, 1236, 1590
SsiI CCGC 3 cut(s) 255, 414, 1812
SspI AATATT 1 cut(s) 913
SspMI CTAG 6 cut(s) 183, 246, 576, 993, 1358, 1849
StyD4I CCNGG 4 cut(s) 81, 111, 117, 1619
StyI CCWWGG 2 cut(s) 176, 1261
TaaI ACNGT 5 cut(s) 72, 687, 761, 878, 1255
TaiI ACGT 2 cut(s) 615, 1180
TaqI TCGA 4 cut(s) 39, 409, 469, 629
TasI AATT 6 cut(s) 130, 446, 503, 1210, 1236, 1590
TatI WGTACW 5 cut(s) 742, 955, 986, 995, 1066
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 1 cut(s) 1339
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 4 cut(s) 131, 316, 766, 1737
TseFI GTSAC 1 cut(s) 1514
TseI GCWGC 6 cut(s) 101, 386, 653, 863, 1136, 1696
Tsp45I GTSAC 1 cut(s) 1514
TspDTI ATGAA 6 cut(s) 552, 855, 1287, 1400, 1491, 1555
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 4 cut(s) 131, 316, 766, 1737
Tth111I GACNNNGTC 2 cut(s) 171, 925
VpaK11BI GGWCC 6 cut(s) 242, 1160, 1258, 1307, 1643, 1724
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 108
XmnI GAANNNNTTC 3 cut(s) 583, 635, 855
XspI CTAG 6 cut(s) 183, 246, 576, 993, 1358, 1849
ZrmI AGTACT 2 cut(s) 988, 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.