Rh7DG069300

Mediator complex subunit 25 von Willebrand factor type A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0017665
Physical Location & Seq
Forward (+)
5339673 .. 5348724
9052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG069300.1

Sequence Viewer

Length: 1530 bp
ATGTTTCCGAAGCCTACTCCCACTGGAACTGGAACTGAAACTGAAACTGATCAAGTTCAAGACCATAATGCCAGAGAAAGGCATTGTATTCTTGTGGCTGCAAGCAACCCTGTGTTGTTTCGAACCGCCGTTCAGATACCGATAATAAGTAATGGCCAATTTTCTTCTGGTACACAAACTCGATGGACTCTTGCTGATGCTCAAGATGTTGCCAAATTATATGCTGAGTGCTCTGTATCTCTTTCTGTGATCTCACCAAAACAGTTTCCAAAACTCAGAGAGATATACAATGCGGGATATATTGATGATCCTGAAGGAACACCTTCCTTCAGCGATGCGAACAATCCTGACGTACTTCTTCTTTTGTCGAAGGACTTCTTAGAGGCTCATGCTGCCCTTATTCAGTATGTTGAAGTGAACATACTGTCAGTTCGGGAGATAAGTCAGATAACAGACTCTGAGCTTGAATATATTGCTTTAGTACATTTCGGAGACAACAGTGAGTTTGATTATATAGGAGATATGTTGGATCCACCCGAGACAGATAAGCAAGACCACCGTGCAGAGAACATTCTCAATGAAACCGAAGAAATGCTTCTTGCAGCAACACAAGACAGTTTTTCACAAGCACCTTCACCCAGCTGTTTCATTCGTGAGACATTGTCCAGTAGCCTTATTCAGTACCCCGAAGTACAGGCCAGGGGATCTGAAGCCGGAGAAAGTAGTACTGCTAGTACTACACAAGCAGAACTCCACTCTCCACAGCATGCGCAACAAATACTTGCAACTCGTACCGTAAGTGGAGTACACAGATCGCAAGGATCTTGCAGGGATCCAAATAGTCCACAAACCACAACTTCTTTAGACACTTTAGCAACAACTTCTGGCAACTCAAGGGGCCCACGAGCATCTTCACGTACTCATAGATCTCAAATAAGTATGGATCCTAATTTCAAACCAAGAAGAAGTCCAAGAATTGCATCAAGAGCTACTGTGGAACAAGGAGGACATGAAGGTGCTTTTGGTCGGTTTGGGGAACCATCTGGTCCACACACATCAGTATCTGTGAAAAGAAGACCTGCACCAAAAGGATTGAAGCCTCTTCCTGCTAGTATGCAATGGAAAGAAGACATATACGAGCTAGCATGGGAGGGTACGATCTTCTCCAAGCATCAAGAAAGGCTTGTTCTAGTCAGTAAGGCAAAGGCGCACAAGATGAAGTCTGCACCAGAGAGCCTTACTACTGGTTGGCCACGACTGCTGAAGATGGGTAATGTTATACCAGAAGCCACTGTTGGCAGGTCATATGAAGGACAAGCCCATGATTTGATCTTTGAGATTACGGGTGACGAGAAAATATATGACCTACTAAATGAGAACGAGCTGTGTATTAGATTCAGCCTGCCTACTCAGTCATTGATAATCACCCCTGCTGAACACAACCACTTCACTGGGAGAGTGTATGCAGGGGGAGGACCTCTAGTGGAGCAACATCTGGTTGCACAATACAATCAACAAAGGCATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007602 GO:0008150 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009639 GO:0009653 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010017 GO:0010026 GO:0010033 GO:0010090 GO:0010091 GO:0010114 GO:0010218 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010927 GO:0016043 GO:0016592 GO:0019219 GO:0019222 GO:0022607 GO:0023052 GO:0030030 GO:0030031 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032502 GO:0032870 GO:0032989 GO:0032991 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048646 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0071214 GO:0071229 GO:0071310 GO:0071395 GO:0071478 GO:0071482 GO:0071489 GO:0071495 GO:0071840 GO:0080090 GO:0080134 GO:0090558 GO:0090626 GO:0097159 GO:0098542 GO:0104004 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1905499 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

509

Amino Acids

56.14

Weight (kDa)

5.65

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 20 - 123 5.4e-11 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017665)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12601 FvH4_5g12610
rosa_chinensis RchiOBHm_Chr7g0184721
rosa_laevigata RLG00000004956
rosa_multiflora Rmu_ssc0000289.1_g000020
rosa_roxburghii Rroxscaffold_3G00269400
rosa_rugosa Rorug06G0464700
rosa_samantha Rh7AG078200 Rh7BG069100 Rh7CG069900 Rh7DG069300
rosa_wichuraiana Rw7G005720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 771
Acc36I ACCTGC 2 cut(s) 1087, 1290
AciI CCGC 2 cut(s) 126, 293
AclWI GGATC 9 cut(s) 302, 524, 537, 712, 827, 829, 840, 938, 951
AcoI YGGCCR 2 cut(s) 154, 1250
AcuI CTGAAG 4 cut(s) 313, 333, 729, 1283
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 5 cut(s) 59, 413, 467, 955, 1096
AjnI CCWGG 1 cut(s) 698
AjuI GAANNNNNNNTTGG 4 cut(s) 1005, 1037, 1278, 1310
AluBI AGCT 5 cut(s) 463, 642, 989, 1141, 1384
AluI AGCT 5 cut(s) 463, 642, 989, 1141, 1384
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 3 cut(s) 486, 533, 650
AlwI GGATC 9 cut(s) 302, 524, 537, 712, 827, 829, 840, 938, 951
AlwNI CAGNNNCTG 2 cut(s) 458, 1064
Ama87I CYCGRG 1 cut(s) 536
AoxI GGCC 4 cut(s) 154, 696, 898, 1250
ApaI GGGCCC 1 cut(s) 902
ApeKI GCWGC 3 cut(s) 98, 392, 602
Asp700I GAANNNNTTC 3 cut(s) 322, 374, 594
AspLEI GCGC 2 cut(s) 772, 1210
AspS9I GGNCC 4 cut(s) 898, 899, 1046, 1475
AsuHPI GGTGA 4 cut(s) 246, 627, 1358, 1417
AsuII TTCGAA 1 cut(s) 121
AsuNHI GCTAGC 1 cut(s) 1141
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 2 cut(s) 1046, 1475
BaeGI GKGCMC 1 cut(s) 902
BaeI ACNNNNGTAYC 2 cut(s) 1044, 1077
BalI TGGCCA 2 cut(s) 156, 1252
BamHI GGATCC 3 cut(s) 529, 832, 943
BanII GRGCYC 1 cut(s) 902
BarI GAAGNNNNNNTAC 2 cut(s) 405, 437
BauI CACGAG 1 cut(s) 903
BbsI GAAGAC 2 cut(s) 1081, 1134
Bbv12I GWGCWC 1 cut(s) 233
BbvI GCAGC 3 cut(s) 85, 379, 614
BccI CCATC 3 cut(s) 177, 1048, 1261
BceAI ACGGC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 700
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 3 cut(s) 486, 533, 650
BfaI CTAG 5 cut(s) 732, 1110, 1142, 1190, 1481
BfuAI ACCTGC 2 cut(s) 1087, 1290
BglII AGATCT 1 cut(s) 926
BisI GCNGC 3 cut(s) 99, 393, 603
BlsI GCNGC 3 cut(s) 100, 394, 604
BmcAI AGTACT 2 cut(s) 727, 736
Bme1390I CCNGG 1 cut(s) 700
Bme18I GGWCC 2 cut(s) 1046, 1475
BmeT110I CYCGRG 1 cut(s) 536
BmgT120I GGNCC 4 cut(s) 898, 899, 1046, 1475
BmiI GGNNCC 6 cut(s) 531, 834, 899, 900, 945, 1038
BmrFI CCNGG 1 cut(s) 700
BmrI ACTGGG 1 cut(s) 1461
BmsI GCATC 5 cut(s) 187, 325, 917, 989, 1180
BmtI GCTAGC 1 cut(s) 1145
BmuI ACTGGG 1 cut(s) 1461
BpiI GAAGAC 2 cut(s) 1081, 1134
Bpu14I TTCGAA 1 cut(s) 121
BpuEI CTTGAG 2 cut(s) 186, 877
BsaAI YACGTR 1 cut(s) 917
BsaJI CCNNGG 1 cut(s) 699
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse1I ACTGG 5 cut(s) 28, 34, 666, 1249, 1456
Bse3DI GCAATG 1 cut(s) 1124
BseBI CCWGG 1 cut(s) 700
BseDI CCNNGG 1 cut(s) 699
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMI GCAATG 1 cut(s) 1124
BseMII CTCAG 4 cut(s) 216, 289, 450, 1424
BseNI ACTGG 5 cut(s) 28, 34, 666, 1249, 1456
BseSI GKGCMC 1 cut(s) 902
BseXI GCAGC 3 cut(s) 85, 379, 614
BseYI CCCAGC 1 cut(s) 638
BsgI GTGCAG 3 cut(s) 582, 1065, 1209
BshFI GGCC 4 cut(s) 156, 698, 900, 1252
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 1 cut(s) 714
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 3 cut(s) 486, 533, 650
BsnI GGCC 4 cut(s) 156, 698, 900, 1252
BsoBI CYCGRG 1 cut(s) 536
Bsp119I TTCGAA 1 cut(s) 121
Bsp120I GGGCCC 1 cut(s) 898
Bsp1286I GDGCHC 2 cut(s) 233, 902
BspACI CCGC 2 cut(s) 126, 293
BspANI GGCC 4 cut(s) 156, 698, 900, 1252
BspCNI CTCAG 4 cut(s) 217, 288, 451, 1423
BspLI GGNNCC 6 cut(s) 531, 834, 899, 900, 945, 1038
BspMI ACCTGC 2 cut(s) 1087, 1290
BspOI GCTAGC 1 cut(s) 1145
BspPI GGATC 9 cut(s) 302, 524, 537, 712, 827, 829, 840, 938, 951
BspT104I TTCGAA 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 1124
BsrI ACTGG 5 cut(s) 28, 34, 666, 1249, 1456
BssECI CCNNGG 1 cut(s) 699
BssSI CACGAG 1 cut(s) 903
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 1 cut(s) 700
Bst4CI ACNGT 8 cut(s) 264, 426, 500, 560, 617, 796, 994, 1294
Bst6I CTCTTC 1 cut(s) 1107
BstBAI YACGTR 1 cut(s) 917
BstBI TTCGAA 1 cut(s) 121
BstC8I GCNNGC 4 cut(s) 103, 768, 1143, 1403
BstDEI CTNAG 5 cut(s) 225, 275, 379, 459, 1410
BstHHI GCGC 2 cut(s) 772, 1210
BstMAI GTCTC 3 cut(s) 486, 533, 650
BstMWI GCNNNNNNNGC 3 cut(s) 392, 986, 1258
BstNI CCWGG 1 cut(s) 700
BstNSI RCATGY 1 cut(s) 770
BstSCI CCNGG 1 cut(s) 698
BstSLI GKGCMC 1 cut(s) 902
BstV1I GCAGC 3 cut(s) 85, 379, 614
BstV2I GAAGAC 2 cut(s) 1081, 1134
BstX2I RGATCY 6 cut(s) 529, 704, 821, 832, 926, 943
BstXI CCANNNNNNTGG 1 cut(s) 1451
BstYI RGATCY 6 cut(s) 529, 704, 821, 832, 926, 943
BsuRI GGCC 4 cut(s) 156, 698, 900, 1252
BtgZI GCGATG 1 cut(s) 348
BtsIMutI CAGTG 4 cut(s) 21, 505, 1290, 1449
BveI ACCTGC 2 cut(s) 1087, 1290
Cac8I GCNNGC 4 cut(s) 103, 768, 1143, 1403
CaiI CAGNNNCTG 2 cut(s) 458, 1064
CfoI GCGC 2 cut(s) 772, 1210
Cfr13I GGNCC 4 cut(s) 898, 899, 1046, 1475
CviAII CATG 5 cut(s) 389, 767, 1010, 1146, 1322
DdeI CTNAG 5 cut(s) 225, 275, 379, 459, 1410
EaeI YGGCCR 2 cut(s) 154, 1250
Eam1104I CTCTTC 1 cut(s) 1107
EarI CTCTTC 1 cut(s) 1107
Eco24I GRGCYC 1 cut(s) 902
Eco47I GGWCC 2 cut(s) 1046, 1475
Eco57I CTGAAG 4 cut(s) 313, 333, 729, 1283
Eco88I CYCGRG 1 cut(s) 536
EcoO109I RGGNCCY 2 cut(s) 898, 1475
EcoRII CCWGG 1 cut(s) 698
EcoT38I GRGCYC 1 cut(s) 902
FaeI CATG 5 cut(s) 392, 770, 1013, 1149, 1325
FalI AAGNNNNNCTT 6 cut(s) 362, 394, 579, 611, 1167, 1199
FatI CATG 5 cut(s) 388, 766, 1009, 1145, 1321
FauI CCCGC 1 cut(s) 286
FauNDI CATATG 1 cut(s) 1306
FbaI TGATCA 1 cut(s) 49
Fnu4HI GCNGC 3 cut(s) 99, 393, 603
FriOI GRGCYC 1 cut(s) 902
Fsp4HI GCNGC 3 cut(s) 99, 393, 603
FspBI CTAG 5 cut(s) 732, 1110, 1142, 1190, 1481
FspI TGCGCA 1 cut(s) 771
GlaI GCGC 2 cut(s) 771, 1209
GluI GCNGC 3 cut(s) 99, 393, 603
GsaI CCCAGC 1 cut(s) 642
HaeIII GGCC 4 cut(s) 156, 698, 900, 1252
HapII CCGG 1 cut(s) 714
HhaI GCGC 2 cut(s) 772, 1210
Hin1II CATG 5 cut(s) 392, 770, 1013, 1149, 1325
Hin6I GCGC 2 cut(s) 770, 1208
HinP1I GCGC 2 cut(s) 770, 1208
HinfI GANTC 3 cut(s) 187, 455, 1395
HpaII CCGG 1 cut(s) 714
HphI GGTGA 4 cut(s) 246, 627, 1358, 1417
Hpy166II GTNNAC 5 cut(s) 173, 418, 808, 845, 1049
Hpy188I TCNGA 7 cut(s) 9, 135, 278, 447, 460, 491, 709
Hpy188III TCNNGA 8 cut(s) 59, 203, 311, 347, 434, 653, 984, 1175
Hpy8I GTNNAC 5 cut(s) 173, 418, 808, 845, 1049
HpyAV CCTTC 7 cut(s) 308, 333, 337, 364, 642, 1007, 1304
HpyCH4III ACNGT 8 cut(s) 264, 426, 500, 560, 617, 796, 994, 1294
HpyCH4IV ACGT 2 cut(s) 351, 916
HpyF10VI GCNNNNNNNGC 3 cut(s) 392, 986, 1258
HpyF3I CTNAG 5 cut(s) 225, 275, 379, 459, 1410
HpySE526I ACGT 2 cut(s) 351, 916
Hsp92II CATG 5 cut(s) 392, 770, 1013, 1149, 1325
HspAI GCGC 2 cut(s) 770, 1208
Ksp22I TGATCA 1 cut(s) 49
LmnI GCTCC 1 cut(s) 1486
Lsp1109I GCAGC 3 cut(s) 85, 379, 614
LweI GCATC 5 cut(s) 187, 325, 917, 989, 1180
MaeI CTAG 5 cut(s) 732, 1110, 1142, 1190, 1481
MaeII ACGT 2 cut(s) 351, 916
MaeIII GTNAC 1 cut(s) 1346
MflI RGATCY 6 cut(s) 529, 704, 821, 832, 926, 943
MhlI GDGCHC 2 cut(s) 233, 902
MlsI TGGCCA 2 cut(s) 156, 1252
MluCI AATT 4 cut(s) 158, 215, 949, 975
MluNI TGGCCA 2 cut(s) 156, 1252
MlyI GAGTC 2 cut(s) 181, 449
MmeI TCCRAC 1 cut(s) 507
MnlI CCTC 6 cut(s) 376, 998, 1110, 1144, 1466, 1488
Mox20I TGGCCA 2 cut(s) 156, 1252
MroXI GAANNNNTTC 3 cut(s) 322, 374, 594
MscI TGGCCA 2 cut(s) 156, 1252
MslI CAYNNNNRTG 1 cut(s) 1014
Msp20I TGGCCA 2 cut(s) 156, 1252
MspA1I CMGCKG 1 cut(s) 642
MspI CCGG 1 cut(s) 714
MspR9I CCNGG 1 cut(s) 700
MvaI CCWGG 1 cut(s) 700
MwoI GCNNNNNNNGC 3 cut(s) 392, 986, 1258
NdeI CATATG 1 cut(s) 1306
NheI GCTAGC 1 cut(s) 1141
NlaIII CATG 5 cut(s) 392, 770, 1013, 1149, 1325
NlaIV GGNNCC 6 cut(s) 531, 834, 899, 900, 945, 1038
NmuCI GTSAC 1 cut(s) 1346
NsbI TGCGCA 1 cut(s) 771
NspI RCATGY 1 cut(s) 770
NspV TTCGAA 1 cut(s) 121
PaeI GCATGC 1 cut(s) 770
PdmI GAANNNNTTC 3 cut(s) 322, 374, 594
PfeI GAWTC 1 cut(s) 1395
PflFI GACNNNGTC 1 cut(s) 661
PkrI GCNGC 3 cut(s) 100, 394, 604
PleI GAGTC 2 cut(s) 181, 449
PpsI GAGTC 2 cut(s) 181, 449
Ppu21I YACGTR 1 cut(s) 917
PpuMI RGGWCCY 1 cut(s) 1475
Psp5II RGGWCCY 1 cut(s) 1475
Psp6I CCWGG 1 cut(s) 698
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 1 cut(s) 698
PspN4I GGNNCC 6 cut(s) 531, 834, 899, 900, 945, 1038
PspOMI GGGCCC 1 cut(s) 898
PspPI GGNCC 4 cut(s) 898, 899, 1046, 1475
PspPPI RGGWCCY 1 cut(s) 1475
PstNI CAGNNNCTG 2 cut(s) 458, 1064
PsuI RGATCY 6 cut(s) 529, 704, 821, 832, 926, 943
PsyI GACNNNGTC 1 cut(s) 661
PvuII CAGCTG 1 cut(s) 642
RseI CAYNNNNRTG 1 cut(s) 1014
SatI GCNGC 3 cut(s) 99, 393, 603
Sau96I GGNCC 4 cut(s) 898, 899, 1046, 1475
ScaI AGTACT 2 cut(s) 727, 736
SchI GAGTC 2 cut(s) 181, 449
ScrFI CCNGG 1 cut(s) 700
SduI GDGCHC 2 cut(s) 233, 902
SfaNI GCATC 5 cut(s) 187, 325, 917, 989, 1180
SfuI TTCGAA 1 cut(s) 121
SinI GGWCC 2 cut(s) 1046, 1475
SmiMI CAYNNNNRTG 1 cut(s) 1014
SmlI CTYRAG 2 cut(s) 201, 892
SmoI CTYRAG 2 cut(s) 201, 892
SphI GCATGC 1 cut(s) 770
Sse9I AATT 4 cut(s) 158, 215, 949, 975
SsiI CCGC 2 cut(s) 126, 293
SspMI CTAG 5 cut(s) 732, 1110, 1142, 1190, 1481
StyD4I CCNGG 1 cut(s) 698
TaaI ACNGT 8 cut(s) 264, 426, 500, 560, 617, 796, 994, 1294
TaiI ACGT 2 cut(s) 354, 919
TaqI TCGA 3 cut(s) 121, 181, 368
TasI AATT 4 cut(s) 158, 215, 949, 975
TatI WGTACW 5 cut(s) 481, 691, 725, 734, 805
TfiI GAWTC 1 cut(s) 1395
TscAI CASTG 4 cut(s) 28, 505, 1297, 1456
TseFI GTSAC 1 cut(s) 1346
TseI GCWGC 3 cut(s) 98, 392, 602
Tsp45I GTSAC 1 cut(s) 1346
TspDTI ATGAA 5 cut(s) 594, 637, 1026, 1232, 1323
TspRI CASTG 4 cut(s) 28, 505, 1297, 1456
Tth111I GACNNNGTC 1 cut(s) 661
VpaK11BI GGWCC 2 cut(s) 1046, 1475
XceI RCATGY 1 cut(s) 770
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XmnI GAANNNNTTC 3 cut(s) 322, 374, 594
XspI CTAG 5 cut(s) 732, 1110, 1142, 1190, 1481
ZrmI AGTACT 2 cut(s) 727, 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.