Rmu_ssc0000289.1_g000020

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000289.1
OG0017665
Physical Location & Seq
Reverse (-)
102014 .. 104676
2663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000289.1_g000020.1.cds

Sequence Viewer

Length: 732 bp
atggcggcagaaaagcggcttatggtggtcgtcgacggaacaacagctatggggccttactggaaatccattctctcagattacctcgaaaagatcatcaggaacataagtggccaaccgtcagctaaccaggatgcttcagctggtgcagcagtatacctgggcctggttcagtataattctcacggacttgataacactggttccttaacgcgaccaagtccttggactagaaatgtggatgggtttcttgaagggctctcggctttacatttttctggtggtggtggtcctaatgaagccgccattgctggggggctggcagaagcacttgtgatgtttccgaagcctactcccactggaactggaactgaaactgaaactgatcaagttcaagaccataatgccagagaaaggcattgtattcttgtggctgcaagcaaccctgtgttgtttcgaaccgccgttcagataccgataataagtaatggccaattttcttctggtacacaaactcgatggactcttgctgatgctcaagatgttgccaaattatatgctgagggaagaaattatcctgaatctgaaggaacagtatcttccttcaaaaatgttaagtacccacattttcctgtactaatatcaaacaagttctcagaggctcgtgctgccctcgataactctcatcctgtgatcaaaactgaatacaatgtctttctcagctcatcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

243

Amino Acids

26.09

Weight (kDa)

5.86

Isoelectric Point (pI)

32.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017665)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12601 FvH4_5g12610
rosa_chinensis RchiOBHm_Chr7g0184721
rosa_laevigata RLG00000004956
rosa_multiflora Rmu_ssc0000289.1_g000020
rosa_roxburghii Rroxscaffold_3G00269400
rosa_rugosa Rorug06G0464700
rosa_samantha Rh7AG078200 Rh7BG069100 Rh7CG069900 Rh7DG069300
rosa_wichuraiana Rw7G005720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 33, 156
AccII CGCG 1 cut(s) 214
AciI CCGC 4 cut(s) 5, 16, 303, 462
AcoI YGGCCR 2 cut(s) 112, 490
AcuI CTGAAG 2 cut(s) 123, 606
AfaI GTAC 3 cut(s) 508, 620, 636
AfiI CCNNNNNNNGG 3 cut(s) 166, 312, 414
AgsI TTSAA 3 cut(s) 254, 395, 607
AjnI CCWGG 3 cut(s) 129, 159, 165
AluBI AGCT 4 cut(s) 47, 125, 143, 723
AluI AGCT 4 cut(s) 47, 125, 143, 723
AoxI GGCC 4 cut(s) 53, 112, 163, 490
ApeKI GCWGC 3 cut(s) 149, 434, 668
AspS9I GGNCC 3 cut(s) 53, 163, 290
AsuII TTCGAA 1 cut(s) 457
AvaII GGWCC 1 cut(s) 290
BalI TGGCCA 2 cut(s) 114, 492
BanII GRGCYC 1 cut(s) 261
BauI CACGAG 1 cut(s) 663
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 3 cut(s) 161, 421, 655
BccI CCATC 2 cut(s) 236, 513
BceAI ACGGC 1 cut(s) 449
BciT130I CCWGG 3 cut(s) 131, 161, 167
BclI TGATCA 2 cut(s) 385, 693
BfaI CTAG 1 cut(s) 231
BisI GCNGC 6 cut(s) 6, 17, 150, 303, 435, 669
BlsI GCNGC 6 cut(s) 7, 18, 151, 304, 436, 670
Bme1390I CCNGG 3 cut(s) 131, 161, 167
Bme18I GGWCC 1 cut(s) 290
BmgT120I GGNCC 3 cut(s) 53, 163, 290
BmiI GGNNCC 2 cut(s) 54, 205
BmrFI CCNGG 3 cut(s) 131, 161, 167
BmsI GCATC 2 cut(s) 124, 523
Bpu10I CCTNAGC 1 cut(s) 561
Bpu14I TTCGAA 1 cut(s) 457
BpuEI CTTGAG 1 cut(s) 522
BsaJI CCNNGG 2 cut(s) 160, 224
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 312, 414
Bse1I ACTGG 4 cut(s) 65, 205, 364, 370
Bse3DI GCAATG 1 cut(s) 306
BseBI CCWGG 3 cut(s) 131, 161, 167
BseDI CCNNGG 2 cut(s) 160, 224
BseGI GGATG 3 cut(s) 139, 247, 685
BseLI CCNNNNNNNGG 3 cut(s) 166, 312, 414
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 3 cut(s) 90, 552, 669
BseNI ACTGG 4 cut(s) 65, 205, 364, 370
BseXI GCAGC 3 cut(s) 161, 421, 655
BseYI CCCAGC 1 cut(s) 311
BsgI GTGCAG 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 214
BshFI GGCC 4 cut(s) 55, 114, 165, 492
BslI CCNNNNNNNGG 3 cut(s) 166, 312, 414
BsnI GGCC 4 cut(s) 55, 114, 165, 492
Bsp119I TTCGAA 1 cut(s) 457
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 3 cut(s) 93, 385, 693
BspACI CCGC 4 cut(s) 5, 16, 303, 462
BspANI GGCC 4 cut(s) 55, 114, 165, 492
BspCNI CTCAG 4 cut(s) 89, 553, 668, 732
BspFNI CGCG 1 cut(s) 214
BspHI TCATGA 1 cut(s) 728
BspLI GGNNCC 2 cut(s) 54, 205
BspT104I TTCGAA 1 cut(s) 457
BsrDI GCAATG 1 cut(s) 306
BsrI ACTGG 4 cut(s) 65, 205, 364, 370
BssECI CCNNGG 2 cut(s) 160, 224
BssMI GATC 3 cut(s) 93, 385, 693
BssNAI GTATAC 1 cut(s) 157
BssSI CACGAG 1 cut(s) 663
BssT1I CCWWGG 1 cut(s) 224
Bst1107I GTATAC 1 cut(s) 157
Bst2BI CACGAG 1 cut(s) 663
Bst2UI CCWGG 3 cut(s) 131, 161, 167
Bst4CI ACNGT 2 cut(s) 120, 595
BstBI TTCGAA 1 cut(s) 457
BstC8I GCNNGC 2 cut(s) 321, 439
BstDEI CTNAG 4 cut(s) 76, 561, 655, 719
BstF5I GGATG 3 cut(s) 139, 247, 685
BstFNI CGCG 1 cut(s) 214
BstKTI GATC 3 cut(s) 96, 388, 696
BstMBI GATC 3 cut(s) 93, 385, 693
BstMWI GCNNNNNNNGC 3 cut(s) 149, 308, 668
BstNI CCWGG 3 cut(s) 131, 161, 167
BstSCI CCNGG 3 cut(s) 129, 159, 165
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 3 cut(s) 161, 421, 655
BstXI CCANNNNNNTGG 1 cut(s) 225
BstZ17I GTATAC 1 cut(s) 157
BsuRI GGCC 4 cut(s) 55, 114, 165, 492
BtsCI GGATG 3 cut(s) 139, 247, 685
BtsIMutI CAGTG 2 cut(s) 198, 357
Cac8I GCNNGC 2 cut(s) 321, 439
CciI TCATGA 1 cut(s) 728
Cfr13I GGNCC 3 cut(s) 53, 163, 290
Csp6I GTAC 3 cut(s) 507, 619, 635
CviAII CATG 1 cut(s) 729
CviQI GTAC 3 cut(s) 507, 619, 635
DdeI CTNAG 4 cut(s) 76, 561, 655, 719
DpnI GATC 3 cut(s) 95, 387, 695
DpnII GATC 3 cut(s) 93, 385, 693
EaeI YGGCCR 2 cut(s) 112, 490
Eco130I CCWWGG 1 cut(s) 224
Eco24I GRGCYC 1 cut(s) 261
Eco47I GGWCC 1 cut(s) 290
Eco57I CTGAAG 2 cut(s) 123, 606
EcoO109I RGGNCCY 1 cut(s) 53
EcoRII CCWGG 3 cut(s) 129, 159, 165
EcoT14I CCWWGG 1 cut(s) 224
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 1 cut(s) 732
FaiI YATR 9 cut(s) 23, 50, 107, 157, 177, 402, 556, 558, 730
FatI CATG 1 cut(s) 728
FbaI TGATCA 2 cut(s) 385, 693
FblI GTMKAC 2 cut(s) 33, 156
Fnu4HI GCNGC 6 cut(s) 6, 17, 150, 303, 435, 669
FokI GGATG 3 cut(s) 146, 254, 672
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 6 cut(s) 6, 17, 150, 303, 435, 669
FspBI CTAG 1 cut(s) 231
GluI GCNGC 6 cut(s) 6, 17, 150, 303, 435, 669
GsaI CCCAGC 1 cut(s) 315
HaeIII GGCC 4 cut(s) 55, 114, 165, 492
Hin1II CATG 1 cut(s) 732
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 2 cut(s) 523, 581
Hpy166II GTNNAC 3 cut(s) 34, 157, 509
Hpy188I TCNGA 5 cut(s) 79, 345, 471, 586, 658
Hpy188III TCNNGA 6 cut(s) 100, 251, 395, 539, 578, 729
Hpy8I GTNNAC 3 cut(s) 34, 157, 509
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 3 cut(s) 248, 581, 613
HpyCH4III ACNGT 2 cut(s) 120, 595
HpyCH4V TGCA 2 cut(s) 149, 437
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 308, 668
HpyF3I CTNAG 4 cut(s) 76, 561, 655, 719
Hsp92II CATG 1 cut(s) 732
Ksp22I TGATCA 2 cut(s) 385, 693
Kzo9I GATC 3 cut(s) 93, 385, 693
Lsp1109I GCAGC 3 cut(s) 161, 421, 655
LweI GCATC 2 cut(s) 124, 523
MaeI CTAG 1 cut(s) 231
MalI GATC 3 cut(s) 95, 387, 695
MboI GATC 3 cut(s) 93, 385, 693
MboII GAAGA 3 cut(s) 492, 579, 591
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 2 cut(s) 114, 492
MluCI AATT 4 cut(s) 178, 494, 551, 571
MluNI TGGCCA 2 cut(s) 114, 492
MlyI GAGTC 1 cut(s) 517
MnlI CCTC 4 cut(s) 95, 556, 652, 683
Mox20I TGGCCA 2 cut(s) 114, 492
MscI TGGCCA 2 cut(s) 114, 492
MseI TTAA 2 cut(s) 209, 615
Msp20I TGGCCA 2 cut(s) 114, 492
MspA1I CMGCKG 1 cut(s) 143
MspR9I CCNGG 3 cut(s) 131, 161, 167
MvaI CCWGG 3 cut(s) 131, 161, 167
MvnI CGCG 1 cut(s) 214
MwoI GCNNNNNNNGC 3 cut(s) 149, 308, 668
NdeII GATC 3 cut(s) 93, 385, 693
NlaIII CATG 1 cut(s) 732
NlaIV GGNNCC 2 cut(s) 54, 205
NmeAIII GCCGAG 1 cut(s) 242
NspV TTCGAA 1 cut(s) 457
PagI TCATGA 1 cut(s) 728
PfeI GAWTC 1 cut(s) 581
PflFI GACNNNGTC 1 cut(s) 219
PkrI GCNGC 6 cut(s) 7, 18, 151, 304, 436, 670
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
Psp6I CCWGG 3 cut(s) 129, 159, 165
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 3 cut(s) 129, 159, 165
PspN4I GGNNCC 2 cut(s) 54, 205
PspPI GGNCC 3 cut(s) 53, 163, 290
PsyI GACNNNGTC 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 3 cut(s) 508, 620, 636
RsaNI GTAC 3 cut(s) 507, 619, 635
SalI GTCGAC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 209, 615
SatI GCNGC 6 cut(s) 6, 17, 150, 303, 435, 669
Sau3AI GATC 3 cut(s) 93, 385, 693
Sau96I GGNCC 3 cut(s) 53, 163, 290
SchI GAGTC 1 cut(s) 517
ScrFI CCNGG 3 cut(s) 131, 161, 167
SduI GDGCHC 1 cut(s) 261
SetI ASST 6 cut(s) 49, 87, 127, 145, 162, 725
SfaNI GCATC 2 cut(s) 124, 523
SfuI TTCGAA 1 cut(s) 457
SgrDI CGTCGACG 1 cut(s) 32
SinI GGWCC 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 537
SmoI CTYRAG 1 cut(s) 537
Sse9I AATT 4 cut(s) 178, 494, 551, 571
SsiI CCGC 4 cut(s) 5, 16, 303, 462
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 3 cut(s) 129, 159, 165
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 2 cut(s) 120, 595
TaqI TCGA 5 cut(s) 33, 87, 457, 517, 675
TasI AATT 4 cut(s) 178, 494, 551, 571
TatI WGTACW 1 cut(s) 634
TauI GCSGC 3 cut(s) 8, 19, 305
TfiI GAWTC 1 cut(s) 581
Tru1I TTAA 2 cut(s) 209, 615
Tru9I TTAA 2 cut(s) 209, 615
TscAI CASTG 2 cut(s) 205, 364
TseI GCWGC 3 cut(s) 149, 434, 668
TspDTI ATGAA 1 cut(s) 312
TspGWI ACGGA 2 cut(s) 51, 201
TspRI CASTG 2 cut(s) 205, 364
Tth111I GACNNNGTC 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 290
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmiI GTMKAC 2 cut(s) 33, 156
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.