Rw7G005720

Mediator complex subunit 25 von Willebrand factor type A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0017665
Physical Location & Seq
Forward (+)
4814103 .. 4819987
5885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G005720.1

Sequence Viewer

Length: 1782 bp
ATGGCGGCAGAAAAGCGGCTTATGGTGGTCGTCGACGGAACAACAGCTATGGGGCCTTACTGGAAATCCATTCTCTCAGATTACCTCGAAAAGATCATCAGGAACATAAGTGGCCAACCGTCAGCTAACCAGGATGCTTCAGCTGGTGCAGCAGTATACCTGGGCCTGGTTCAGTATAATTCTCACGGACTTGATAACACTGGTTCCTTAACGCGACCAAGTCCTTGGACTAGAAATGTGGATGGGTTTCTTGAAGGGCTCTCGGCTTTACATTTTTCTGGTGGTGGTGGTCCTAATGAAGCCGCCATTGCTGGGGGGCTGGCAGAAGCACTTGTGATGTTTCCGAAGCCTACTCCCACTGGAACTGGAACTGAAACTGAAACTGATCAAGTTCAAGACCATAATGCCAGAGAAAGGCATTGTATTCTTGTGGCTGCAAGCAACCCTGTGTTGTTTCGAACCACCGTTCAGATACCGATAATAAGTAATGGCCAATTTTCTTCTGGTACACAAACTCGATGGACTCTTGCTGATGCTCAAGATGTTGCCAAATTATATGCTGAGTGCTCTGTATCTCTTTCTGTGATCTCACCAAAACAGTTTCCAAAACTCAGAGAGATATACAATGCGGGATATATTGATGATCCTGAAGGAACACCTTCCTTCAGCGATGCGAACAATCCTGACGTACTTCTTCTTTTGTCGAAGGACTTCTTAGAGGCTCATGCTGCCCTTATTCAGTATGTTGAAGTGAACATACTGTCAGTTCGGGAGATAAGTCAGATAACAGACTCTGAGCTTGAATATATTGCTTTAGAAGTTTCTGATTTACCAGTACATTTCGGAGACAACAGTGAGTTTGATTATATAGGAGATATGTTGGATCCACCCGAGACAGATAAGCAAGACCATCGTGCAGAGAACATTCTCAATGAAACCGAAGAAATGCTTCTTGCAGCAACACAAGACAGTTTTTCACAAGCACCTTCACCCAGCTGTTTCATTCGTGAGACATTGTCCAGTAGCCTTATTCAGTACCCCGAAGTACAGGCAACGGGATCTGAAGCCGGAGAAAGTAGTACTGCTAGTACTACACAAGCAGAACTCCACTCTCCACAGCATGCGCAACAAATACTTGCAACTCGTACCGTAAGTGGAGTACACAGATCGCAAGGATCTTGCAGGGATCCAAATAGTCTACAAACCACAACTTCTTTAGACACTTTAGCAACAACTTCTGGCAACTCAAGGGGTCCACGAGCATCTTCACGTACTCAAAGATCTCAAATAAGTATGGATCCTAATTTCAAACCAAGAAGAAGTCCAAGAATTGCATCAAGAGCACCTGTGGAACAAGGAGGACATGAAGGTGCTTTTGGTCGGTTTGGGGAACCATCTGGTCCACACACATCAGTATCTGTGAAAAGAAAACCTGCACGAAAAGGATTGAAGCCTCTTCCTGCTAGTATGCAATGGAAAGAAGACATATACGAGCTAGCATGGGAGGGTACGATCTTCTCCAAGCATCAAGAACGGCTTGTTCTAGTCAGTAAGGCAAAGGCGCACAAGATGAAGTCTGCACCAGAGAGCCTTACTACTGGTTGGCCACGACTGCTGAAGATGGGTAATGTTATACCAGAAGCCACTGTTGGCAGGTCATATGAAGGACAAGCCCATGATTTGATCTTTGAGATTACGGGTGACGAGAAAATATATGACCTACTAAATGAGAACCAGCTGATTCAGCCTGCCTACTCAGTCATTGATAATCAGCCCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007602 GO:0008150 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009639 GO:0009653 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010017 GO:0010026 GO:0010033 GO:0010090 GO:0010091 GO:0010114 GO:0010218 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010927 GO:0016043 GO:0016592 GO:0019219 GO:0019222 GO:0022607 GO:0023052 GO:0030030 GO:0030031 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032502 GO:0032870 GO:0032989 GO:0032991 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048646 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0071214 GO:0071229 GO:0071310 GO:0071395 GO:0071478 GO:0071482 GO:0071489 GO:0071495 GO:0071840 GO:0080090 GO:0080134 GO:0090558 GO:0090626 GO:0097159 GO:0098542 GO:0104004 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1905499 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

593

Amino Acids

64.54

Weight (kDa)

5.21

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 4 - 235 1.6e-32 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017665)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12601 FvH4_5g12610
rosa_chinensis RchiOBHm_Chr7g0184721
rosa_laevigata RLG00000004956
rosa_multiflora Rmu_ssc0000289.1_g000020
rosa_roxburghii Rroxscaffold_3G00269400
rosa_rugosa Rorug06G0464700
rosa_samantha Rh7AG078200 Rh7BG069100 Rh7CG069900 Rh7DG069300
rosa_wichuraiana Rw7G005720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1125
Acc36I ACCTGC 2 cut(s) 1441, 1644
AccI GTMKAC 3 cut(s) 33, 156, 1198
AccII CGCG 1 cut(s) 214
AciI CCGC 4 cut(s) 5, 16, 303, 629
AclWI GGATC 9 cut(s) 638, 878, 891, 1066, 1181, 1183, 1194, 1292, 1305
AcoI YGGCCR 3 cut(s) 112, 490, 1604
AcuI CTGAAG 5 cut(s) 123, 649, 669, 1083, 1637
AfiI CCNNNNNNNGG 3 cut(s) 166, 312, 414
AgsI TTSAA 6 cut(s) 254, 395, 749, 803, 1309, 1450
AjnI CCWGG 3 cut(s) 129, 159, 165
AjuI GAANNNNNNNTTGG 4 cut(s) 1359, 1391, 1632, 1664
AluBI AGCT 7 cut(s) 47, 125, 143, 799, 996, 1495, 1738
AluI AGCT 7 cut(s) 47, 125, 143, 799, 996, 1495, 1738
Alw21I GWGCWC 2 cut(s) 569, 1345
Alw26I GTCTC 3 cut(s) 840, 887, 1004
AlwI GGATC 9 cut(s) 638, 878, 891, 1066, 1181, 1183, 1194, 1292, 1305
AlwNI CAGNNNCTG 2 cut(s) 794, 1418
Ama87I CYCGRG 1 cut(s) 890
AoxI GGCC 5 cut(s) 53, 112, 163, 490, 1604
ApeKI GCWGC 4 cut(s) 149, 434, 728, 956
Asp700I GAANNNNTTC 3 cut(s) 658, 710, 948
AspLEI GCGC 2 cut(s) 1126, 1564
AspS9I GGNCC 5 cut(s) 53, 163, 290, 1253, 1400
AsuHPI GGTGA 3 cut(s) 582, 981, 1712
AsuII TTCGAA 1 cut(s) 457
AsuNHI GCTAGC 1 cut(s) 1495
AvaI CYCGRG 1 cut(s) 890
AvaII GGWCC 3 cut(s) 290, 1253, 1400
BaeI ACNNNNGTAYC 2 cut(s) 1398, 1431
BalI TGGCCA 3 cut(s) 114, 492, 1606
BamHI GGATCC 3 cut(s) 883, 1186, 1297
BanII GRGCYC 1 cut(s) 261
BarI GAAGNNNNNNTAC 2 cut(s) 741, 773
BauI CACGAG 1 cut(s) 1257
BbsI GAAGAC 1 cut(s) 1488
Bbv12I GWGCWC 2 cut(s) 569, 1345
BbvI GCAGC 4 cut(s) 161, 421, 715, 968
BccI CCATC 5 cut(s) 236, 513, 918, 1402, 1615
BceAI ACGGC 1 cut(s) 1550
BcgI CGANNNNNNTGC 2 cut(s) 893, 927
BciT130I CCWGG 3 cut(s) 131, 161, 167
BclI TGATCA 1 cut(s) 385
BcoDI GTCTC 3 cut(s) 840, 887, 1004
BfaI CTAG 5 cut(s) 231, 1086, 1464, 1496, 1544
BfuAI ACCTGC 2 cut(s) 1441, 1644
BglII AGATCT 1 cut(s) 1280
BisI GCNGC 7 cut(s) 6, 17, 150, 303, 435, 729, 957
BlsI GCNGC 7 cut(s) 7, 18, 151, 304, 436, 730, 958
BmcAI AGTACT 2 cut(s) 1081, 1090
Bme1390I CCNGG 3 cut(s) 131, 161, 167
Bme18I GGWCC 3 cut(s) 290, 1253, 1400
BmeT110I CYCGRG 1 cut(s) 890
BmgT120I GGNCC 5 cut(s) 53, 163, 290, 1253, 1400
BmiI GGNNCC 7 cut(s) 54, 205, 885, 1188, 1254, 1299, 1392
BmrFI CCNGG 3 cut(s) 131, 161, 167
BmsI GCATC 6 cut(s) 124, 523, 661, 1271, 1343, 1534
BmtI GCTAGC 1 cut(s) 1499
BpiI GAAGAC 1 cut(s) 1488
Bpu14I TTCGAA 1 cut(s) 457
BpuEI CTTGAG 2 cut(s) 522, 1231
BsaAI YACGTR 1 cut(s) 1271
BsaJI CCNNGG 2 cut(s) 160, 224
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 312, 414
Bse1I ACTGG 7 cut(s) 65, 205, 364, 370, 833, 1020, 1603
Bse3DI GCAATG 2 cut(s) 306, 1478
BseBI CCWGG 3 cut(s) 131, 161, 167
BseDI CCNNGG 2 cut(s) 160, 224
BseGI GGATG 2 cut(s) 139, 247
BseLI CCNNNNNNNGG 3 cut(s) 166, 312, 414
BseMI GCAATG 2 cut(s) 306, 1478
BseMII CTCAG 5 cut(s) 90, 552, 625, 786, 1770
BseNI ACTGG 7 cut(s) 65, 205, 364, 370, 833, 1020, 1603
BseXI GCAGC 4 cut(s) 161, 421, 715, 968
BseYI CCCAGC 2 cut(s) 311, 992
BsgI GTGCAG 4 cut(s) 168, 936, 1419, 1563
Bsh1236I CGCG 1 cut(s) 214
BshFI GGCC 5 cut(s) 55, 114, 165, 492, 1606
BsiHKAI GWGCWC 2 cut(s) 569, 1345
BsiHKCI CYCGRG 1 cut(s) 890
BsiSI CCGG 1 cut(s) 1068
BslI CCNNNNNNNGG 3 cut(s) 166, 312, 414
BsmAI GTCTC 3 cut(s) 840, 887, 1004
BsnI GGCC 5 cut(s) 55, 114, 165, 492, 1606
BsoBI CYCGRG 1 cut(s) 890
Bsp119I TTCGAA 1 cut(s) 457
Bsp1286I GDGCHC 3 cut(s) 261, 569, 1345
BspACI CCGC 4 cut(s) 5, 16, 303, 629
BspANI GGCC 5 cut(s) 55, 114, 165, 492, 1606
BspCNI CTCAG 5 cut(s) 89, 553, 624, 787, 1769
BspFNI CGCG 1 cut(s) 214
BspLI GGNNCC 7 cut(s) 54, 205, 885, 1188, 1254, 1299, 1392
BspMI ACCTGC 2 cut(s) 1441, 1644
BspOI GCTAGC 1 cut(s) 1499
BspPI GGATC 9 cut(s) 638, 878, 891, 1066, 1181, 1183, 1194, 1292, 1305
BspT104I TTCGAA 1 cut(s) 457
BsrDI GCAATG 2 cut(s) 306, 1478
BsrI ACTGG 7 cut(s) 65, 205, 364, 370, 833, 1020, 1603
BssECI CCNNGG 2 cut(s) 160, 224
BssNAI GTATAC 1 cut(s) 157
BssSI CACGAG 1 cut(s) 1257
BssT1I CCWWGG 1 cut(s) 224
Bst1107I GTATAC 1 cut(s) 157
Bst2BI CACGAG 1 cut(s) 1257
Bst2UI CCWGG 3 cut(s) 131, 161, 167
Bst4CI ACNGT 8 cut(s) 120, 466, 600, 762, 854, 971, 1150, 1648
Bst6I CTCTTC 1 cut(s) 1461
BstBAI YACGTR 1 cut(s) 1271
BstBI TTCGAA 1 cut(s) 457
BstC8I GCNNGC 5 cut(s) 321, 439, 1122, 1497, 1749
BstDEI CTNAG 6 cut(s) 76, 561, 611, 715, 795, 1756
BstF5I GGATG 2 cut(s) 139, 247
BstFNI CGCG 1 cut(s) 214
BstHHI GCGC 2 cut(s) 1126, 1564
BstMAI GTCTC 3 cut(s) 840, 887, 1004
BstMWI GCNNNNNNNGC 6 cut(s) 149, 308, 728, 1340, 1612, 1744
BstNI CCWGG 3 cut(s) 131, 161, 167
BstNSI RCATGY 1 cut(s) 1124
BstSCI CCNGG 3 cut(s) 129, 159, 165
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 4 cut(s) 161, 421, 715, 968
BstV2I GAAGAC 1 cut(s) 1488
BstX2I RGATCY 6 cut(s) 883, 1058, 1175, 1186, 1280, 1297
BstXI CCANNNNNNTGG 1 cut(s) 225
BstYI RGATCY 6 cut(s) 883, 1058, 1175, 1186, 1280, 1297
BstZ17I GTATAC 1 cut(s) 157
BsuRI GGCC 5 cut(s) 55, 114, 165, 492, 1606
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 2 cut(s) 139, 247
BtsIMutI CAGTG 4 cut(s) 198, 357, 859, 1644
BveI ACCTGC 2 cut(s) 1441, 1644
Cac8I GCNNGC 5 cut(s) 321, 439, 1122, 1497, 1749
CaiI CAGNNNCTG 2 cut(s) 794, 1418
CfoI GCGC 2 cut(s) 1126, 1564
Cfr13I GGNCC 5 cut(s) 53, 163, 290, 1253, 1400
CviAII CATG 5 cut(s) 725, 1121, 1364, 1500, 1676
DdeI CTNAG 6 cut(s) 76, 561, 611, 715, 795, 1756
EaeI YGGCCR 3 cut(s) 112, 490, 1604
Eam1104I CTCTTC 1 cut(s) 1461
EarI CTCTTC 1 cut(s) 1461
Eco130I CCWWGG 1 cut(s) 224
Eco24I GRGCYC 1 cut(s) 261
Eco47I GGWCC 3 cut(s) 290, 1253, 1400
Eco57I CTGAAG 5 cut(s) 123, 649, 669, 1083, 1637
Eco88I CYCGRG 1 cut(s) 890
EcoO109I RGGNCCY 1 cut(s) 53
EcoRII CCWGG 3 cut(s) 129, 159, 165
EcoT14I CCWWGG 1 cut(s) 224
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 5 cut(s) 728, 1124, 1367, 1503, 1679
FalI AAGNNNNNCTT 6 cut(s) 698, 730, 933, 965, 1521, 1553
FatI CATG 5 cut(s) 724, 1120, 1363, 1499, 1675
FauI CCCGC 1 cut(s) 622
FauNDI CATATG 1 cut(s) 1660
FbaI TGATCA 1 cut(s) 385
FblI GTMKAC 3 cut(s) 33, 156, 1198
Fnu4HI GCNGC 7 cut(s) 6, 17, 150, 303, 435, 729, 957
FokI GGATG 2 cut(s) 146, 254
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 7 cut(s) 6, 17, 150, 303, 435, 729, 957
FspBI CTAG 5 cut(s) 231, 1086, 1464, 1496, 1544
FspI TGCGCA 1 cut(s) 1125
GlaI GCGC 2 cut(s) 1125, 1563
GluI GCNGC 7 cut(s) 6, 17, 150, 303, 435, 729, 957
GsaI CCCAGC 2 cut(s) 315, 996
HaeIII GGCC 5 cut(s) 55, 114, 165, 492, 1606
HapII CCGG 1 cut(s) 1068
HhaI GCGC 2 cut(s) 1126, 1564
Hin1II CATG 5 cut(s) 728, 1124, 1367, 1503, 1679
Hin6I GCGC 2 cut(s) 1124, 1562
HinP1I GCGC 2 cut(s) 1124, 1562
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 3 cut(s) 523, 791, 1741
HpaII CCGG 1 cut(s) 1068
HphI GGTGA 3 cut(s) 582, 981, 1712
Hpy166II GTNNAC 8 cut(s) 34, 157, 509, 754, 1162, 1199, 1256, 1403
Hpy188I TCNGA 9 cut(s) 79, 345, 471, 614, 783, 796, 826, 845, 1063
Hpy8I GTNNAC 8 cut(s) 34, 157, 509, 754, 1162, 1199, 1256, 1403
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 8 cut(s) 248, 644, 669, 673, 700, 996, 1361, 1658
HpyCH4III ACNGT 8 cut(s) 120, 466, 600, 762, 854, 971, 1150, 1648
HpyCH4IV ACGT 2 cut(s) 687, 1270
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 308, 728, 1340, 1612, 1744
HpyF3I CTNAG 6 cut(s) 76, 561, 611, 715, 795, 1756
HpySE526I ACGT 2 cut(s) 687, 1270
Hsp92II CATG 5 cut(s) 728, 1124, 1367, 1503, 1679
HspAI GCGC 2 cut(s) 1124, 1562
Ksp22I TGATCA 1 cut(s) 385
Lsp1109I GCAGC 4 cut(s) 161, 421, 715, 968
LweI GCATC 6 cut(s) 124, 523, 661, 1271, 1343, 1534
MaeI CTAG 5 cut(s) 231, 1086, 1464, 1496, 1544
MaeII ACGT 2 cut(s) 687, 1270
MaeIII GTNAC 1 cut(s) 1700
MboII GAAGA 9 cut(s) 492, 686, 953, 1257, 1329, 1448, 1493, 1507, 1630
MflI RGATCY 6 cut(s) 883, 1058, 1175, 1186, 1280, 1297
MhlI GDGCHC 3 cut(s) 261, 569, 1345
MlsI TGGCCA 3 cut(s) 114, 492, 1606
MluCI AATT 5 cut(s) 178, 494, 551, 1303, 1329
MluNI TGGCCA 3 cut(s) 114, 492, 1606
MlyI GAGTC 2 cut(s) 517, 785
MmeI TCCRAC 1 cut(s) 861
MnlI CCTC 5 cut(s) 95, 712, 1352, 1464, 1498
Mox20I TGGCCA 3 cut(s) 114, 492, 1606
MroXI GAANNNNTTC 3 cut(s) 658, 710, 948
MscI TGGCCA 3 cut(s) 114, 492, 1606
MseI TTAA 1 cut(s) 209
MslI CAYNNNNRTG 1 cut(s) 1368
Msp20I TGGCCA 3 cut(s) 114, 492, 1606
MspA1I CMGCKG 3 cut(s) 143, 996, 1738
MspI CCGG 1 cut(s) 1068
MspR9I CCNGG 3 cut(s) 131, 161, 167
MvaI CCWGG 3 cut(s) 131, 161, 167
MvnI CGCG 1 cut(s) 214
MwoI GCNNNNNNNGC 6 cut(s) 149, 308, 728, 1340, 1612, 1744
NdeI CATATG 1 cut(s) 1660
NheI GCTAGC 1 cut(s) 1495
NlaIII CATG 5 cut(s) 728, 1124, 1367, 1503, 1679
NlaIV GGNNCC 7 cut(s) 54, 205, 885, 1188, 1254, 1299, 1392
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 1 cut(s) 1700
NsbI TGCGCA 1 cut(s) 1125
NspI RCATGY 1 cut(s) 1124
NspV TTCGAA 1 cut(s) 457
PaeI GCATGC 1 cut(s) 1124
PdmI GAANNNNTTC 3 cut(s) 658, 710, 948
PfeI GAWTC 1 cut(s) 1741
PflFI GACNNNGTC 2 cut(s) 219, 1015
PkrI GCNGC 7 cut(s) 7, 18, 151, 304, 436, 730, 958
PleI GAGTC 2 cut(s) 517, 785
PpsI GAGTC 2 cut(s) 517, 785
Ppu21I YACGTR 1 cut(s) 1271
Psp6I CCWGG 3 cut(s) 129, 159, 165
PspFI CCCAGC 2 cut(s) 311, 992
PspGI CCWGG 3 cut(s) 129, 159, 165
PspN4I GGNNCC 7 cut(s) 54, 205, 885, 1188, 1254, 1299, 1392
PspPI GGNCC 5 cut(s) 53, 163, 290, 1253, 1400
PstNI CAGNNNCTG 2 cut(s) 794, 1418
PsuI RGATCY 6 cut(s) 883, 1058, 1175, 1186, 1280, 1297
PsyI GACNNNGTC 2 cut(s) 219, 1015
PvuII CAGCTG 3 cut(s) 143, 996, 1738
RseI CAYNNNNRTG 1 cut(s) 1368
SalI GTCGAC 1 cut(s) 32
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 7 cut(s) 6, 17, 150, 303, 435, 729, 957
Sau96I GGNCC 5 cut(s) 53, 163, 290, 1253, 1400
ScaI AGTACT 2 cut(s) 1081, 1090
SchI GAGTC 2 cut(s) 517, 785
ScrFI CCNGG 3 cut(s) 131, 161, 167
SduI GDGCHC 3 cut(s) 261, 569, 1345
SfaNI GCATC 6 cut(s) 124, 523, 661, 1271, 1343, 1534
SfuI TTCGAA 1 cut(s) 457
SgrDI CGTCGACG 1 cut(s) 32
SinI GGWCC 3 cut(s) 290, 1253, 1400
SmiMI CAYNNNNRTG 1 cut(s) 1368
SmlI CTYRAG 2 cut(s) 537, 1246
SmoI CTYRAG 2 cut(s) 537, 1246
SphI GCATGC 1 cut(s) 1124
Sse9I AATT 5 cut(s) 178, 494, 551, 1303, 1329
SsiI CCGC 4 cut(s) 5, 16, 303, 629
SspMI CTAG 5 cut(s) 231, 1086, 1464, 1496, 1544
StyD4I CCNGG 3 cut(s) 129, 159, 165
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 8 cut(s) 120, 466, 600, 762, 854, 971, 1150, 1648
TaiI ACGT 2 cut(s) 690, 1273
TaqI TCGA 5 cut(s) 33, 87, 457, 517, 704
TasI AATT 5 cut(s) 178, 494, 551, 1303, 1329
TatI WGTACW 5 cut(s) 835, 1045, 1079, 1088, 1159
TauI GCSGC 3 cut(s) 8, 19, 305
TfiI GAWTC 1 cut(s) 1741
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TscAI CASTG 4 cut(s) 205, 364, 859, 1651
TseFI GTSAC 1 cut(s) 1700
TseI GCWGC 4 cut(s) 149, 434, 728, 956
Tsp45I GTSAC 1 cut(s) 1700
TspDTI ATGAA 6 cut(s) 312, 948, 991, 1380, 1586, 1677
TspGWI ACGGA 2 cut(s) 51, 201
TspRI CASTG 4 cut(s) 205, 364, 859, 1651
Tth111I GACNNNGTC 2 cut(s) 219, 1015
VpaK11BI GGWCC 3 cut(s) 290, 1253, 1400
XceI RCATGY 1 cut(s) 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmiI GTMKAC 3 cut(s) 33, 156, 1198
XmnI GAANNNNTTC 3 cut(s) 658, 710, 948
XspI CTAG 5 cut(s) 231, 1086, 1464, 1496, 1544
ZrmI AGTACT 2 cut(s) 1081, 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.