Rorug06G0464700

Mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0017665
Physical Location & Seq
Reverse (-)
58939475 .. 58942002
2528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0464700.1

Sequence Viewer

Length: 2247 bp
ATGCTTCTCCAACTTCTGCTAATAGGGTCAGTACTAATAGCCACAGCAGCACAAGCACAACTAGCTGATCAATATGTGAAACCTGGTTGCGAAGCTACCTGTGGAAATGTTAACATACCATACCCTTTCGGTACCAGAGAAGCTTATTGTTACCATGATAAAGAGTTCCTTGTAACTTGCAACTCCACATATGACCCTCCGAAACTATTTCTGTCCGGAGGCACTGTTGAGGTCACAAACATTTCTGTTGATGGCAAGTTGAATATCATGCAATATATAGCTCACGATTGCTATGAATCATCTGGTTTCCGTATCAAGAACAATTCTCCATGGCTCCGCCTTTCAAAATTCATCATCTCCAACACCGATAATAAGTTCATTGCAGTTGGATGTGACACTTATGCAGAAATCCGAGGCTTCCAGGGCGAAATCAAGAAGTACACAGGTGGCTGCATATCAACATGTGACAGCATTGATTATGTTGCCAATGATTCATGTTCCGGTATAGGGTGTTGCCAGACATCAATTGCTAAAGGTGTGAGCTATTTTGATGTGTCTGTGGGCAGTTATCAAAATCATACAGATGTTTGGTCTTTCAATCCATGCAGCTTCGCCTTTATTGTGGAAGAAAGCAAGTTCAACTTCTCCAGAGCTATGCTCAAGGATTTAAAGAATGTAACAGTACTTCCTGTTGTGCTTGATTGGTCAATTGGAAATGAGACTTGCAAGCAAGTTGAAGGCAAAATGAAAGCTGGCAGCGCGTGCCAGGGGAACAGCACATGTGTTGATGTCGAAAATGGGTCTGGATACCGATGCAAGTGCAAGGATGGTTATCAAGGAAACCCATACCTCTCTAATGGTTGTCAAGATATTGATGAATGTGAGGATCCAAAACTCAGTAACTGTGCACAAAAATGTGTAAATAAGGAGGGAAGTCATTACTGTTCTTGCCGCAAAGGGTACCATGGCAATGGTATAGCAGGGGAAGAAGGTTGCATAGCTAACAGAGCATTAGTTGTTCAAATCACTGTTGGAATTGCTGTCGGCCTCATATCGTTGCTAACGTGTTGCTCTTGGTTATATCTCGGCTACAGAAGATGGAAACTCATTAAGCTGAAGGAGAGATTCTTCAGACAAAATGGAGGATTAATGTTACAACAACAACTTTCAGAACGACAAGGTGGTGCCACTAACCAAAATGCAAAAATCTTTACTGCAGAAGAACTTGAGAAGGCCACCAACAACTACCATGAGACTAGAATCGTTGGCAAAGGAGGCTACGGTACAGTGTACAAAGGAATTTTATCAGATGGAAGAGTAGTTGCAATCAAGAAATCCAAAATAGTTGATCAAAGCCAAATAGAGCAATTCATTAATGAAGTACTGGTGCTTTCCCAAATCAATCACAGGAATGTGGTGAAGCTTTTGGGTTGTTGTTTCGAGACAGAAGTCCCACGACTAGTTTATGAGTTTGTCACCAATGGTACGCTATTTGACTACATCCACAACAAAAGCAAGGCATCCAACTTTGCTTGGGAAACTCGTTTAAGGATAGCTGCAGAAGCTGCTGGAGTGCTATCATACTTGCACTCAGCTGCTTCTGTCCCTATTATCCATAGAGATGTAAAGTCCACAAATATACTTTTAGATGAAACCCTTACAGCAAAAGTGTCTGATTTTGGTGCCTCCAGATTGGTACCATTGGATCAAACTGAGTTATCGACAATGGTACAAGGCACGCTCGGGTACTTAGACCCTGAATACTTTCAAACAAGCCAATTGACTGACAAGAGTGATGTCTATAGCTTTGGGGTTGTCCTTGTGGAGCTACTAACAGGAAAGAAGGCGCTTTCGTTCGACAGGCAAGAGGAGGATAGAAATCTAGCAATGTATTTTCTTTCCGCCCTGAAAGAAAACCGCTTGGTACAAGTGGTTGATGAGTGTGTTCTGAATGGGGCAAATGACGAGCAGCTTAAGGAAGTTTCTAACCTTGCAAAGAGGTGTTTGAAAGTGAAAGGAGAAGAAAGACCAACCATGATGGAAGTAGCAAGCGAGCTTGAGGGACTAAGAAGGATGGCAATGCATCATCCCTGGGTTAATAATGATGAATCCAATGCACAGGAGAATGAAAGCTTGCTTGGTGAAATATCACTGGAAACGTTTAGTCATGGAGGTGGTGGCGATACGAGTTCTGGGTATGATACAATGAGGAACCACATCATATTACCAGTAAATGATGGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007602 GO:0008150 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009639 GO:0009653 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010017 GO:0010026 GO:0010033 GO:0010090 GO:0010091 GO:0010114 GO:0010218 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010927 GO:0016043 GO:0016592 GO:0019219 GO:0019222 GO:0022607 GO:0023052 GO:0030030 GO:0030031 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032502 GO:0032870 GO:0032989 GO:0032991 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048646 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0071214 GO:0071229 GO:0071310 GO:0071395 GO:0071478 GO:0071482 GO:0071489 GO:0071495 GO:0071840 GO:0080090 GO:0080134 GO:0090558 GO:0090626 GO:0097159 GO:0098542 GO:0104004 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1905499 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

748

Amino Acids

82.75

Weight (kDa)

5.74

Isoelectric Point (pI)

28.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 30 - 85 1.5e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 290 - 324 2.2e-07 Calcium-binding EGF domain
Pkinase PF00069 416 - 683 8.8e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 419 - 686 1.3e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017665)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12601 FvH4_5g12610
rosa_chinensis RchiOBHm_Chr7g0184721
rosa_laevigata RLG00000004956
rosa_multiflora Rmu_ssc0000289.1_g000020
rosa_roxburghii Rroxscaffold_3G00269400
rosa_rugosa Rorug06G0464700
rosa_samantha Rh7AG078200 Rh7BG069100 Rh7CG069900 Rh7DG069300
rosa_wichuraiana Rw7G005720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 3 cut(s) 131, 960, 1696
AccB1I GGYRCC 5 cut(s) 131, 960, 1184, 1682, 1696
AccII CGCG 1 cut(s) 761
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 4 cut(s) 337, 952, 1902, 1918
AclI AACGTT 1 cut(s) 2159
AclWI GGATC 3 cut(s) 881, 894, 1713
AcsI RAATTY 2 cut(s) 347, 1299
AcuI CTGAAG 2 cut(s) 1114, 1136
AfiI CCNNNNNNNGG 1 cut(s) 507
AflII CTTAAG 1 cut(s) 1973
AflIII ACRYGT 3 cut(s) 461, 779, 1064
AgsI TTSAA 8 cut(s) 262, 345, 598, 640, 737, 1022, 1769, 2008
AhlI ACTAGT 1 cut(s) 1459
AjnI CCWGG 4 cut(s) 82, 420, 765, 2090
AjuI GAANNNNNNNTTGG 2 cut(s) 2121, 2153
Alw21I GWGCWC 1 cut(s) 910
Alw26I GTCTC 3 cut(s) 713, 1247, 1436
Alw44I GTGCAC 1 cut(s) 906
AlwI GGATC 3 cut(s) 881, 894, 1713
AlwNI CAGNNNCTG 2 cut(s) 903, 1565
Ama87I CYCGRG 1 cut(s) 1742
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 2 cut(s) 1045, 1233
ApaLI GTGCAC 1 cut(s) 906
ApeKI GCWGC 8 cut(s) 47, 450, 606, 756, 1556, 1565, 1595, 1969
ApoI RAATTY 2 cut(s) 347, 1299
AseI ATTAAT 2 cut(s) 1148, 1374
Asp700I GAANNNNTTC 1 cut(s) 1764
Asp718I GGTACC 3 cut(s) 131, 960, 1696
AspLEI GCGC 2 cut(s) 761, 1849
AsuHPI GGTGA 3 cut(s) 1429, 1468, 2153
AvaI CYCGRG 1 cut(s) 1742
BaeGI GKGCMC 1 cut(s) 910
BamHI GGATCC 1 cut(s) 886
BanI GGYRCC 5 cut(s) 131, 960, 1184, 1682, 1696
BarI GAAGNNNNNNTAC 2 cut(s) 669, 701
Bbv12I GWGCWC 1 cut(s) 910
BbvI GCAGC 8 cut(s) 59, 437, 618, 768, 1543, 1552, 1582, 1981
BccI CCATC 7 cut(s) 245, 821, 1092, 1304, 2032, 2068, 2231
BcgI CGANNNNNNTGC 2 cut(s) 801, 835
BciT130I CCWGG 4 cut(s) 84, 422, 767, 2092
BciVI GTATCC 1 cut(s) 800
BclI TGATCA 2 cut(s) 67, 1348
BcoDI GTCTC 3 cut(s) 713, 1247, 1436
BcuI ACTAGT 1 cut(s) 1459
BfaI CTAG 4 cut(s) 62, 1257, 1460, 1883
BfmI CTRYAG 4 cut(s) 1090, 1215, 1557, 1801
BfoI RGCGCY 1 cut(s) 1850
BfrI CTTAAG 1 cut(s) 1973
BfuI GTATCC 1 cut(s) 800
BisI GCNGC 9 cut(s) 48, 451, 607, 757, 952, 1557, 1566, 1596, 1970
BlsI GCNGC 9 cut(s) 49, 452, 608, 758, 953, 1558, 1567, 1597, 1971
BmcAI AGTACT 3 cut(s) 33, 684, 1383
Bme1390I CCNGG 4 cut(s) 84, 422, 767, 2092
BmeT110I CYCGRG 1 cut(s) 1742
BmiI GGNNCC 8 cut(s) 133, 335, 888, 962, 1186, 1684, 1698, 2213
BmrFI CCNGG 4 cut(s) 84, 422, 767, 2092
BmsI GCATC 3 cut(s) 803, 1529, 2092
BoxI GACNNNNGTC 1 cut(s) 1448
BplI GAGNNNNNCTC 2 cut(s) 642, 674
BpmI CTGGAG 3 cut(s) 631, 1590, 1672
BpuEI CTTGAG 3 cut(s) 644, 1247, 2078
BsaBI GATNNNNATC 2 cut(s) 831, 1878
BsaJI CCNNGG 7 cut(s) 329, 412, 421, 766, 964, 2090, 2091
BsaWI WCCGGW 2 cut(s) 215, 500
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 1 cut(s) 507
Bse1I ACTGG 3 cut(s) 1389, 2157, 2228
Bse3DI GCAATG 4 cut(s) 378, 976, 1893, 2085
Bse8I GATNNNNATC 2 cut(s) 831, 1878
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 4 cut(s) 84, 422, 767, 2092
BseDI CCNNGG 7 cut(s) 329, 412, 421, 766, 964, 2090, 2091
BseGI GGATG 6 cut(s) 395, 832, 1500, 1520, 2079, 2086
BseJI GATNNNNATC 2 cut(s) 831, 1878
BseLI CCNNNNNNNGG 1 cut(s) 507
BseMI GCAATG 4 cut(s) 378, 976, 1893, 2085
BseMII CTCAG 3 cut(s) 910, 1605, 1704
BseNI ACTGG 3 cut(s) 1389, 2157, 2228
BseRI GAGGAG 1 cut(s) 1883
BseSI GKGCMC 1 cut(s) 910
BseXI GCAGC 8 cut(s) 59, 437, 618, 768, 1543, 1552, 1582, 1981
Bsh1236I CGCG 1 cut(s) 761
BshFI GGCC 2 cut(s) 1047, 1235
BshNI GGYRCC 5 cut(s) 131, 960, 1184, 1682, 1696
BsiHKAI GWGCWC 1 cut(s) 910
BsiHKCI CYCGRG 1 cut(s) 1742
BsiSI CCGG 2 cut(s) 216, 501
BslFI GGGAC 3 cut(s) 1436, 1589, 2076
BslI CCNNNNNNNGG 1 cut(s) 507
BsmAI GTCTC 3 cut(s) 713, 1247, 1436
BsmFI GGGAC 3 cut(s) 1436, 1589, 2076
BsnI GGCC 2 cut(s) 1047, 1235
BsoBI CYCGRG 1 cut(s) 1742
Bsp1286I GDGCHC 1 cut(s) 910
Bsp13I TCCGGA 1 cut(s) 215
Bsp1407I TGTACA 1 cut(s) 1290
Bsp143I GATC 4 cut(s) 67, 886, 1348, 1705
Bsp19I CCATGG 2 cut(s) 329, 964
BspACI CCGC 4 cut(s) 337, 952, 1902, 1918
BspANI GGCC 2 cut(s) 1047, 1235
BspCNI CTCAG 3 cut(s) 909, 1604, 1705
BspEI TCCGGA 1 cut(s) 215
BspFNI CGCG 1 cut(s) 761
BspLI GGNNCC 8 cut(s) 133, 335, 888, 962, 1186, 1684, 1698, 2213
BspMAI CTGCAG 2 cut(s) 1219, 1561
BspPI GGATC 3 cut(s) 881, 894, 1713
BspT107I GGYRCC 5 cut(s) 131, 960, 1184, 1682, 1696
BspTI CTTAAG 1 cut(s) 1973
BsrDI GCAATG 4 cut(s) 378, 976, 1893, 2085
BsrGI TGTACA 1 cut(s) 1290
BsrI ACTGG 3 cut(s) 1389, 2157, 2228
BssECI CCNNGG 7 cut(s) 329, 412, 421, 766, 964, 2090, 2091
BssMI GATC 4 cut(s) 67, 886, 1348, 1705
BssT1I CCWWGG 2 cut(s) 329, 964
Bst2UI CCWGG 4 cut(s) 84, 422, 767, 2092
Bst4CI ACNGT 7 cut(s) 226, 682, 905, 944, 1030, 1283, 1288
Bst6I CTCTTC 1 cut(s) 1309
BstAFI CTTAAG 1 cut(s) 1973
BstAPI GCANNNNNTGC 2 cut(s) 762, 1565
BstAUI TGTACA 1 cut(s) 1290
BstC8I GCNNGC 7 cut(s) 728, 754, 763, 1739, 2050, 2054, 2135
BstDEI CTNAG 5 cut(s) 896, 1591, 1713, 1750, 2066
BstDSI CCRYGG 2 cut(s) 329, 964
BstF5I GGATG 6 cut(s) 395, 832, 1500, 1520, 2079, 2086
BstFNI CGCG 1 cut(s) 761
BstH2I RGCGCY 1 cut(s) 1850
BstHHI GCGC 2 cut(s) 761, 1849
BstKTI GATC 4 cut(s) 70, 889, 1351, 1708
BstMAI GTCTC 3 cut(s) 713, 1247, 1436
BstMBI GATC 4 cut(s) 67, 886, 1348, 1705
BstNI CCWGG 4 cut(s) 84, 422, 767, 2092
BstNSI RCATGY 2 cut(s) 465, 783
BstPAI GACNNNNGTC 1 cut(s) 1448
BstSCI CCNGG 4 cut(s) 82, 420, 765, 2090
BstSFI CTRYAG 4 cut(s) 1090, 1215, 1557, 1801
BstSLI GKGCMC 1 cut(s) 910
BstUI CGCG 1 cut(s) 761
BstV1I GCAGC 8 cut(s) 59, 437, 618, 768, 1543, 1552, 1582, 1981
BstX2I RGATCY 1 cut(s) 886
BstXI CCANNNNNNTGG 1 cut(s) 971
BstYI RGATCY 1 cut(s) 886
BsuI GTATCC 1 cut(s) 800
BsuRI GGCC 2 cut(s) 1047, 1235
BtgI CCRYGG 2 cut(s) 329, 964
BtsCI GGATG 6 cut(s) 395, 832, 1500, 1520, 2079, 2086
BtsIMutI CAGTG 4 cut(s) 222, 1026, 1293, 2150
Cac8I GCNNGC 7 cut(s) 728, 754, 763, 1739, 2050, 2054, 2135
CaiI CAGNNNCTG 2 cut(s) 903, 1565
CfoI GCGC 2 cut(s) 761, 1849
CsiI ACCWGGT 1 cut(s) 82
DdeI CTNAG 5 cut(s) 896, 1591, 1713, 1750, 2066
DpnI GATC 4 cut(s) 69, 888, 1350, 1707
DpnII GATC 4 cut(s) 67, 886, 1348, 1705
DraI TTTAAA 1 cut(s) 669
Eam1104I CTCTTC 1 cut(s) 1309
EarI CTCTTC 1 cut(s) 1309
EciI GGCGGA 2 cut(s) 326, 1891
Eco130I CCWWGG 2 cut(s) 329, 964
Eco57I CTGAAG 2 cut(s) 1114, 1136
Eco88I CYCGRG 1 cut(s) 1742
EcoRII CCWGG 4 cut(s) 82, 420, 765, 2090
EcoT14I CCWWGG 2 cut(s) 329, 964
EcoT22I ATGCAT 1 cut(s) 2085
ErhI CCWWGG 2 cut(s) 329, 964
FalI AAGNNNNNCTT 4 cut(s) 153, 185, 626, 658
FaqI GGGAC 3 cut(s) 1436, 1589, 2076
FauNDI CATATG 1 cut(s) 190
FbaI TGATCA 2 cut(s) 67, 1348
Fnu4HI GCNGC 9 cut(s) 48, 451, 607, 757, 952, 1557, 1566, 1596, 1970
FokI GGATG 6 cut(s) 402, 839, 1487, 1507, 2073, 2086
Fsp4HI GCNGC 9 cut(s) 48, 451, 607, 757, 952, 1557, 1566, 1596, 1970
FspBI CTAG 4 cut(s) 62, 1257, 1460, 1883
GlaI GCGC 2 cut(s) 760, 1848
GluI GCNGC 9 cut(s) 48, 451, 607, 757, 952, 1557, 1566, 1596, 1970
GsuI CTGGAG 3 cut(s) 631, 1590, 1672
HaeII RGCGCY 1 cut(s) 1850
HaeIII GGCC 2 cut(s) 1047, 1235
HapII CCGG 2 cut(s) 216, 501
HhaI GCGC 2 cut(s) 761, 1849
Hin6I GCGC 2 cut(s) 759, 1847
HinP1I GCGC 2 cut(s) 759, 1847
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 3 cut(s) 141, 1421, 2131
HinfI GANTC 5 cut(s) 296, 491, 1125, 1260, 2108
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 2 cut(s) 216, 501
HphI GGTGA 3 cut(s) 1429, 1468, 2153
Hpy166II GTNNAC 5 cut(s) 112, 441, 908, 1291, 1632
Hpy188I TCNGA 7 cut(s) 201, 413, 1133, 1171, 1309, 1675, 1950
Hpy8I GTNNAC 5 cut(s) 112, 441, 908, 1291, 1632
HpyAV CCTTC 6 cut(s) 731, 983, 1111, 1225, 1837, 2064
HpyCH4III ACNGT 7 cut(s) 226, 682, 905, 944, 1030, 1283, 1288
HpyCH4IV ACGT 2 cut(s) 1064, 2159
HpyF3I CTNAG 5 cut(s) 896, 1591, 1713, 1750, 2066
HpySE526I ACGT 2 cut(s) 1064, 2159
HspAI GCGC 2 cut(s) 759, 1847
Kpn2I TCCGGA 1 cut(s) 215
KpnI GGTACC 3 cut(s) 135, 964, 1700
Ksp22I TGATCA 2 cut(s) 67, 1348
KspAI GTTAAC 1 cut(s) 112
Kzo9I GATC 4 cut(s) 67, 886, 1348, 1705
LmnI GCTCC 2 cut(s) 339, 1825
Lsp1109I GCAGC 8 cut(s) 59, 437, 618, 768, 1543, 1552, 1582, 1981
LweI GCATC 3 cut(s) 803, 1529, 2092
MabI ACCWGGT 1 cut(s) 82
MaeI CTAG 4 cut(s) 62, 1257, 1460, 1883
MaeII ACGT 2 cut(s) 1064, 2159
MaeIII GTNAC 9 cut(s) 149, 172, 232, 392, 464, 676, 899, 1152, 1474
MalI GATC 4 cut(s) 69, 888, 1350, 1707
MboI GATC 4 cut(s) 67, 886, 1348, 1705
MboII GAAGA 7 cut(s) 638, 998, 1107, 1120, 1232, 1326, 2033
MfeI CAATTG 3 cut(s) 525, 708, 1778
MflI RGATCY 1 cut(s) 886
MhlI GDGCHC 1 cut(s) 910
MluCI AATT 8 cut(s) 322, 347, 525, 708, 1035, 1299, 1367, 1778
MmeI TCCRAC 5 cut(s) 34, 367, 384, 1012, 1548
Mph1103I ATGCAT 1 cut(s) 2085
MroI TCCGGA 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 1764
MseI TTAA 8 cut(s) 111, 668, 1110, 1148, 1374, 1547, 1974, 2097
MslI CAYNNNNRTG 6 cut(s) 582, 913, 969, 1410, 1620, 2172
MspA1I CMGCKG 1 cut(s) 1595
MspCI CTTAAG 1 cut(s) 1973
MspI CCGG 2 cut(s) 216, 501
MspR9I CCNGG 4 cut(s) 84, 422, 767, 2092
MunI CAATTG 3 cut(s) 525, 708, 1778
MvaI CCWGG 4 cut(s) 84, 422, 767, 2092
MvnI CGCG 1 cut(s) 761
NcoI CCATGG 2 cut(s) 329, 964
NdeI CATATG 1 cut(s) 190
NdeII GATC 4 cut(s) 67, 886, 1348, 1705
NlaIV GGNNCC 8 cut(s) 133, 335, 888, 962, 1186, 1684, 1698, 2213
NmeAIII GCCGAG 1 cut(s) 1065
NmuCI GTSAC 4 cut(s) 232, 392, 464, 1474
NsiI ATGCAT 1 cut(s) 2085
NspI RCATGY 2 cut(s) 465, 783
PasI CCCWGGG 1 cut(s) 2091
PciI ACATGT 2 cut(s) 461, 779
PcsI WCGNNNNNNNCGW 1 cut(s) 1061
PdmI GAANNNNTTC 1 cut(s) 1764
PfeI GAWTC 5 cut(s) 296, 491, 1125, 1260, 2108
PkrI GCNGC 9 cut(s) 49, 452, 608, 758, 953, 1558, 1567, 1597, 1971
PscI ACATGT 2 cut(s) 461, 779
PshAI GACNNNNGTC 1 cut(s) 1448
PshBI ATTAAT 2 cut(s) 1148, 1374
Psp1406I AACGTT 1 cut(s) 2159
Psp6I CCWGG 4 cut(s) 82, 420, 765, 2090
PspGI CCWGG 4 cut(s) 82, 420, 765, 2090
PspN4I GGNNCC 8 cut(s) 133, 335, 888, 962, 1186, 1684, 1698, 2213
PstI CTGCAG 2 cut(s) 1219, 1561
PstNI CAGNNNCTG 2 cut(s) 903, 1565
PsuI RGATCY 1 cut(s) 886
PvuII CAGCTG 1 cut(s) 1595
RseI CAYNNNNRTG 6 cut(s) 582, 913, 969, 1410, 1620, 2172
SaqAI TTAA 8 cut(s) 111, 668, 1110, 1148, 1374, 1547, 1974, 2097
SatI GCNGC 9 cut(s) 48, 451, 607, 757, 952, 1557, 1566, 1596, 1970
Sau3AI GATC 4 cut(s) 67, 886, 1348, 1705
ScaI AGTACT 3 cut(s) 33, 684, 1383
ScrFI CCNGG 4 cut(s) 84, 422, 767, 2092
SduI GDGCHC 1 cut(s) 910
SexAI ACCWGGT 1 cut(s) 82
SfaNI GCATC 3 cut(s) 803, 1529, 2092
SfcI CTRYAG 4 cut(s) 1090, 1215, 1557, 1801
SmiMI CAYNNNNRTG 6 cut(s) 582, 913, 969, 1410, 1620, 2172
SmlI CTYRAG 4 cut(s) 659, 1226, 1973, 2057
SmoI CTYRAG 4 cut(s) 659, 1226, 1973, 2057
SpeI ACTAGT 1 cut(s) 1459
Sse9I AATT 8 cut(s) 322, 347, 525, 708, 1035, 1299, 1367, 1778
SsiI CCGC 4 cut(s) 337, 952, 1902, 1918
SspMI CTAG 4 cut(s) 62, 1257, 1460, 1883
StyD4I CCNGG 4 cut(s) 82, 420, 765, 2090
StyI CCWWGG 2 cut(s) 329, 964
TaaI ACNGT 7 cut(s) 226, 682, 905, 944, 1030, 1283, 1288
TaiI ACGT 2 cut(s) 1067, 2162
TaqI TCGA 4 cut(s) 792, 1440, 1721, 1857
TasI AATT 8 cut(s) 322, 347, 525, 708, 1035, 1299, 1367, 1778
TatI WGTACW 5 cut(s) 31, 438, 682, 1290, 1381
TauI GCSGC 1 cut(s) 954
TfiI GAWTC 5 cut(s) 296, 491, 1125, 1260, 2108
Tru1I TTAA 8 cut(s) 111, 668, 1110, 1148, 1374, 1547, 1974, 2097
Tru9I TTAA 8 cut(s) 111, 668, 1110, 1148, 1374, 1547, 1974, 2097
TscAI CASTG 4 cut(s) 229, 1033, 1293, 2157
TseFI GTSAC 4 cut(s) 232, 392, 464, 1474
TseI GCWGC 8 cut(s) 47, 450, 606, 756, 1556, 1565, 1595, 1969
Tsp45I GTSAC 4 cut(s) 232, 392, 464, 1474
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 4 cut(s) 229, 1033, 1293, 2157
Vha464I CTTAAG 1 cut(s) 1973
VneI GTGCAC 1 cut(s) 906
VspI ATTAAT 2 cut(s) 1148, 1374
XapI RAATTY 2 cut(s) 347, 1299
XceI RCATGY 2 cut(s) 465, 783
XmnI GAANNNNTTC 1 cut(s) 1764
XspI CTAG 4 cut(s) 62, 1257, 1460, 1883
ZrmI AGTACT 3 cut(s) 33, 684, 1383
Zsp2I ATGCAT 1 cut(s) 2085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.