FvH4_5g13520

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003696
Physical Location & Seq
Forward (+)
7643263 .. 7648058
4796 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13520.t5

Sequence Viewer

Length: 1545 bp
ATGGAGTCTTGGCGCTGTTCTTCTGAAGGGAAGAGTTTTGCACCTGATGAAACAGTTTCTCATACTGATTCACTTGCTAGAAATAGAAGTGCTTTGATGGGTTGTGATTTGAAAATCCCATGTACTTTTGAAAGTCAAGGTTTTGGGGAATTGGGTTTGCCAGAAATGTTGGGCAAACATCTACCAAGAAGTACAACAAGTATGGGTTCCCCAAATCCATTTTTTAGGGAAGATGAATCCTCTTCAAAGCTTTCAAGCTCTGCTGTTGAATCCAACAGCAGGGATTATTCACTCTTTGATTTGAAACTAGATGCTTATACTTCTAAGTCAACGAAAGGAGCTTCCCTTTTTTCTTCATCCGAGTCATCTACACCTATGAAGAGAATGAGGGTATCAGGGGTGTACTCCCAGACTGCTTATTGCCAGGTTCATGGCTGCAACAAGGACCTCAGCTCCTCTAAGGACTACCACAAGAGGCATAAAGTTTGTGAGGCTCACTCGAAGACCGCCAAAGTTATTGTCAAAGGCATAGAACAGAGGTTTTGTCAGCAATGTAGTAGGTTCCATTTGCTGGCTGAATTTGATGATGGTAAGCGCAGCTGTCGTAAACGCCTTGCCGGACACAATGAGCGCCGGAGAAAGCCTCAAGTTAATATTCATTCTGGAAGAGATGGGAGGTTGCTTCAGCCATATAACGGCAGCAGTTTTCCAGGGACTATGTTCACAGCTGCGTCTTTCGTCTGCCAAGATATACTCCCTAGTAGCATTTTGGATGCTGAGAATTACAGAACAAGTGACTGGCGCAGGCGTGTAAAAGTGGAAGGTGGCACTCAGCATAGGCCTTTGTCAGGGGTGCCTGAAACAAATGGGCAACTGCATTTGAAACCAGTCTTTTCTTATGATATTGGAAAACATTGTCCACCAGCTCATGTAGATGACACCAATAATCTTCCTAGTGGAAGCATACTCAGTGAGACTGGTAGTCAGTATCCACATGATACGAGAAGCTTAAATTCTGGTTCACGTTCTATTTATAATGAGGGTGCATTAGGAAGTGAAGGCTTCAATGATTTCCACACAACAGCAACAACCGTGCAACAATTATCGCGCATTCCAGAGTCCGGTTGTGCTCACTCTTTTCTGTCATCTCAATCACAGAACTCTACAAGCTATGCTTCGGGAATTCCTATTGCTCACCCCTCAGTAATGCCAGTCAGCCAAACACATTACAACCCCAGCCAAGTTTCTGATAAACTTTGTTCTCAGGGTTCCACAAGTGGGGTGTCAAATAAATTTCCTTCTTCGGGGGGTCCCATGACGATATCTAATGGAAGAGATGCTAATGATTTTGGAACAAATGGGATTTTCCAAGGATCACAGTTTTTGAACACCAAATATCGTCTATCATGTGATGGTGGGACTACTGTTGACTTGCTTCAACTTTCCTCAGAGCTTCAACGAGTTGATGATCAACGGCATTCCCTGCAGGTGAAGCAGGAATATGACGCTTTCTGCTGCCTCCAAAGTCCGAATCATGTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

515

Amino Acids

56.46

Weight (kDa)

8.5

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 141 - 214 1.3e-32 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1035
AarI CACCTGC 1 cut(s) 1477
Acc36I ACCTGC 1 cut(s) 1477
AccB1I GGYRCC 1 cut(s) 853
AccB7I CCANNNNNTGG 1 cut(s) 571
AccII CGCG 1 cut(s) 1108
AciI CCGC 1 cut(s) 507
AclWI GGATC 1 cut(s) 1381
AcsI RAATTY 4 cut(s) 578, 1012, 1182, 1292
AcuI CTGAAG 2 cut(s) 45, 668
AfaI GTAC 3 cut(s) 124, 193, 404
AfiI CCNNNNNNNGG 7 cut(s) 279, 571, 695, 848, 1121, 1278, 1304
AhdI GACNNNNNGTC 1 cut(s) 981
AjnI CCWGG 2 cut(s) 423, 709
Alw21I GWGCWC 1 cut(s) 1132
Alw26I GTCTC 1 cut(s) 968
AlwI GGATC 1 cut(s) 1381
AoxI GGCC 1 cut(s) 839
ApeKI GCWGC 5 cut(s) 435, 597, 699, 728, 1515
ApoI RAATTY 4 cut(s) 578, 1012, 1182, 1292
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
AspLEI GCGC 5 cut(s) 15, 597, 633, 804, 1110
AspS9I GGNCC 2 cut(s) 445, 1310
AsuHPI GGTGA 2 cut(s) 1187, 1501
AvaII GGWCC 2 cut(s) 445, 1310
BanI GGYRCC 1 cut(s) 853
BbsI GAAGAC 1 cut(s) 509
Bbv12I GWGCWC 1 cut(s) 1132
BbvCI CCTCAGC 1 cut(s) 449
BbvI GCAGC 5 cut(s) 422, 609, 711, 715, 1502
BccI CCATC 4 cut(s) 91, 581, 665, 1406
BceAI ACGGC 2 cut(s) 712, 1490
BciT130I CCWGG 2 cut(s) 425, 711
BciVI GTATCC 1 cut(s) 999
BclI TGATCA 1 cut(s) 1468
BcoDI GTCTC 1 cut(s) 968
BfaI CTAG 4 cut(s) 78, 308, 759, 954
BfmI CTRYAG 1 cut(s) 1484
BfoI RGCGCY 2 cut(s) 16, 634
BfuAI ACCTGC 1 cut(s) 1477
BfuI GTATCC 1 cut(s) 999
BisI GCNGC 5 cut(s) 436, 598, 700, 729, 1516
BlsI GCNGC 5 cut(s) 437, 599, 701, 730, 1517
Bme1390I CCNGG 2 cut(s) 425, 711
Bme18I GGWCC 2 cut(s) 445, 1310
BmeRI GACNNNNNGTC 1 cut(s) 981
BmgT120I GGNCC 2 cut(s) 445, 1310
BmiI GGNNCC 6 cut(s) 208, 563, 855, 1270, 1311, 1312
BmrFI CCNGG 2 cut(s) 425, 711
BmsI GCATC 3 cut(s) 301, 763, 1327
BpiI GAAGAC 1 cut(s) 509
BplI GAGNNNNNCTC 4 cut(s) 482, 514, 628, 660
Bpu10I CCTNAGC 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 630
BsaJI CCNNGG 2 cut(s) 710, 1369
BsaWI WCCGGW 1 cut(s) 1121
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 7 cut(s) 279, 571, 695, 848, 1121, 1278, 1304
Bse1I ACTGG 4 cut(s) 803, 887, 982, 1211
Bse3DI GCAATG 1 cut(s) 557
BseBI CCWGG 2 cut(s) 425, 711
BseDI CCNNGG 2 cut(s) 710, 1369
BseGI GGATG 2 cut(s) 356, 778
BseLI CCNNNNNNNGG 7 cut(s) 279, 571, 695, 848, 1121, 1278, 1304
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 7 cut(s) 463, 768, 845, 982, 1215, 1277, 1461
BseNI ACTGG 4 cut(s) 803, 887, 982, 1211
BseRI GAGGAG 1 cut(s) 445
BseXI GCAGC 5 cut(s) 422, 609, 711, 715, 1502
BseYI CCCAGC 1 cut(s) 1235
Bsh1236I CGCG 1 cut(s) 1108
BshFI GGCC 1 cut(s) 841
BshNI GGYRCC 1 cut(s) 853
BsiHKAI GWGCWC 1 cut(s) 1132
BsiSI CCGG 3 cut(s) 618, 634, 1122
BslFI GGGAC 3 cut(s) 727, 1296, 1432
BslI CCNNNNNNNGG 7 cut(s) 279, 571, 695, 848, 1121, 1278, 1304
BsmAI GTCTC 1 cut(s) 968
BsmFI GGGAC 3 cut(s) 727, 1296, 1432
BsmI GAATGC 2 cut(s) 1110, 1477
BsnI GGCC 1 cut(s) 841
Bsp1286I GDGCHC 1 cut(s) 1132
Bsp143I GATC 2 cut(s) 1373, 1468
BspACI CCGC 1 cut(s) 507
BspANI GGCC 1 cut(s) 841
BspCNI CTCAG 7 cut(s) 462, 769, 844, 981, 1214, 1276, 1460
BspFNI CGCG 1 cut(s) 1108
BspLI GGNNCC 6 cut(s) 208, 563, 855, 1270, 1311, 1312
BspMAI CTGCAG 1 cut(s) 1488
BspMI ACCTGC 1 cut(s) 1477
BspPI GGATC 1 cut(s) 1381
BspT107I GGYRCC 1 cut(s) 853
BsrDI GCAATG 1 cut(s) 557
BsrI ACTGG 4 cut(s) 803, 887, 982, 1211
BssECI CCNNGG 2 cut(s) 710, 1369
BssMI GATC 2 cut(s) 1373, 1468
BssT1I CCWWGG 1 cut(s) 1369
Bst2UI CCWGG 2 cut(s) 425, 711
Bst4CI ACNGT 4 cut(s) 55, 1093, 1380, 1426
Bst6I CTCTTC 5 cut(s) 26, 247, 374, 661, 1327
BstAPI GCANNNNNTGC 1 cut(s) 1483
BstC8I GCNNGC 2 cut(s) 573, 806
BstDEI CTNAG 9 cut(s) 324, 449, 459, 777, 831, 968, 1201, 1263, 1447
BstENI CCTNNNNNAGG 1 cut(s) 846
BstF5I GGATG 2 cut(s) 356, 778
BstFNI CGCG 1 cut(s) 1108
BstH2I RGCGCY 2 cut(s) 16, 634
BstHHI GCGC 5 cut(s) 15, 597, 633, 804, 1110
BstKTI GATC 2 cut(s) 1376, 1471
BstMAI GTCTC 1 cut(s) 968
BstMBI GATC 2 cut(s) 1373, 1468
BstMWI GCNNNNNNNGC 2 cut(s) 1483, 1492
BstNI CCWGG 2 cut(s) 425, 711
BstSCI CCNGG 2 cut(s) 423, 709
BstSFI CTRYAG 1 cut(s) 1484
BstUI CGCG 1 cut(s) 1108
BstV1I GCAGC 5 cut(s) 422, 609, 711, 715, 1502
BstV2I GAAGAC 1 cut(s) 509
BstXI CCANNNNNNTGG 1 cut(s) 431
BsuI GTATCC 1 cut(s) 999
BsuRI GGCC 1 cut(s) 841
BtsCI GGATG 2 cut(s) 356, 778
BtsIMutI CAGTG 1 cut(s) 976
BveI ACCTGC 1 cut(s) 1477
Cac8I GCNNGC 2 cut(s) 573, 806
CfoI GCGC 5 cut(s) 15, 597, 633, 804, 1110
Cfr13I GGNCC 2 cut(s) 445, 1310
CseI GACGC 2 cut(s) 720, 1514
Csp6I GTAC 3 cut(s) 123, 192, 403
CviAII CATG 7 cut(s) 120, 431, 929, 995, 1315, 1407, 1535
CviQI GTAC 3 cut(s) 123, 192, 403
DdeI CTNAG 9 cut(s) 324, 449, 459, 777, 831, 968, 1201, 1263, 1447
DpnI GATC 2 cut(s) 1375, 1470
DpnII GATC 2 cut(s) 1373, 1468
DriI GACNNNNNGTC 1 cut(s) 981
Eam1104I CTCTTC 5 cut(s) 26, 247, 374, 661, 1327
Eam1105I GACNNNNNGTC 1 cut(s) 981
EarI CTCTTC 5 cut(s) 26, 247, 374, 661, 1327
Eco130I CCWWGG 1 cut(s) 1369
Eco147I AGGCCT 1 cut(s) 841
Eco32I GATATC 1 cut(s) 1323
Eco47I GGWCC 2 cut(s) 445, 1310
Eco57I CTGAAG 2 cut(s) 45, 668
EcoNI CCTNNNNNAGG 1 cut(s) 846
EcoO109I RGGNCCY 2 cut(s) 445, 1310
EcoRI GAATTC 1 cut(s) 1182
EcoRII CCWGG 2 cut(s) 423, 709
EcoRV GATATC 1 cut(s) 1323
EcoT14I CCWWGG 1 cut(s) 1369
ErhI CCWWGG 1 cut(s) 1369
FaeI CATG 7 cut(s) 123, 434, 932, 998, 1318, 1410, 1538
FalI AAGNNNNNCTT 2 cut(s) 1159, 1191
FaqI GGGAC 3 cut(s) 727, 1296, 1432
FatI CATG 7 cut(s) 119, 430, 928, 994, 1314, 1406, 1534
FbaI TGATCA 1 cut(s) 1468
Fnu4HI GCNGC 5 cut(s) 436, 598, 700, 729, 1516
FokI GGATG 2 cut(s) 343, 785
Fsp4HI GCNGC 5 cut(s) 436, 598, 700, 729, 1516
FspBI CTAG 4 cut(s) 78, 308, 759, 954
GlaI GCGC 5 cut(s) 14, 596, 632, 803, 1109
GluI GCNGC 5 cut(s) 436, 598, 700, 729, 1516
GsaI CCCAGC 1 cut(s) 1239
HaeII RGCGCY 2 cut(s) 16, 634
HaeIII GGCC 1 cut(s) 841
HapII CCGG 3 cut(s) 618, 634, 1122
HgaI GACGC 2 cut(s) 720, 1514
HhaI GCGC 5 cut(s) 15, 597, 633, 804, 1110
Hin1II CATG 7 cut(s) 123, 434, 932, 998, 1318, 1410, 1538
Hin6I GCGC 5 cut(s) 13, 595, 631, 802, 1108
HinP1I GCGC 5 cut(s) 13, 595, 631, 802, 1108
HincII GTYRAC 2 cut(s) 330, 1429
HindII GTYRAC 2 cut(s) 330, 1429
HindIII AAGCTT 2 cut(s) 248, 1006
HinfI GANTC 7 cut(s) 5, 68, 236, 269, 362, 1118, 1531
HpaII CCGG 3 cut(s) 618, 634, 1122
HphI GGTGA 2 cut(s) 1187, 1501
Hpy166II GTNNAC 7 cut(s) 330, 403, 608, 723, 920, 1022, 1429
Hpy188I TCNGA 5 cut(s) 25, 361, 1249, 1450, 1530
Hpy188III TCNNGA 3 cut(s) 663, 1115, 1179
Hpy8I GTNNAC 7 cut(s) 330, 403, 608, 723, 920, 1022, 1429
HpyAV CCTTC 4 cut(s) 20, 815, 1052, 1308
HpyCH4III ACNGT 4 cut(s) 55, 1093, 1380, 1426
HpyCH4IV ACGT 1 cut(s) 1024
HpyCH4V TGCA 6 cut(s) 41, 438, 877, 1046, 1096, 1486
HpyF10VI GCNNNNNNNGC 2 cut(s) 1483, 1492
HpyF3I CTNAG 9 cut(s) 324, 449, 459, 777, 831, 968, 1201, 1263, 1447
HpySE526I ACGT 1 cut(s) 1024
Hsp92II CATG 7 cut(s) 123, 434, 932, 998, 1318, 1410, 1538
HspAI GCGC 5 cut(s) 13, 595, 631, 802, 1108
KflI GGGWCCC 1 cut(s) 1310
Ksp22I TGATCA 1 cut(s) 1468
Kzo9I GATC 2 cut(s) 1373, 1468
LmnI GCTCC 2 cut(s) 338, 458
Lsp1109I GCAGC 5 cut(s) 422, 609, 711, 715, 1502
LweI GCATC 3 cut(s) 301, 763, 1327
MaeI CTAG 4 cut(s) 78, 308, 759, 954
MaeII ACGT 1 cut(s) 1024
MaeIII GTNAC 1 cut(s) 794
MalI GATC 2 cut(s) 1375, 1470
MboI GATC 2 cut(s) 1373, 1468
MhlI GDGCHC 1 cut(s) 1132
MluCI AATT 7 cut(s) 149, 578, 781, 1012, 1100, 1182, 1292
MlyI GAGTC 3 cut(s) 14, 371, 1127
MmeI TCCRAC 1 cut(s) 297
MseI TTAA 2 cut(s) 651, 1010
MslI CAYNNNNRTG 1 cut(s) 933
MspA1I CMGCKG 2 cut(s) 600, 728
MspI CCGG 3 cut(s) 618, 634, 1122
MspR9I CCNGG 2 cut(s) 425, 711
Mva1269I GAATGC 2 cut(s) 1110, 1477
MvaI CCWGG 2 cut(s) 425, 711
MvnI CGCG 1 cut(s) 1108
MwoI GCNNNNNNNGC 2 cut(s) 1483, 1492
NdeII GATC 2 cut(s) 1373, 1468
NlaIII CATG 7 cut(s) 123, 434, 932, 998, 1318, 1410, 1538
NlaIV GGNNCC 6 cut(s) 208, 563, 855, 1270, 1311, 1312
NmuCI GTSAC 1 cut(s) 794
PaqCI CACCTGC 1 cut(s) 1477
PceI AGGCCT 1 cut(s) 841
PctI GAATGC 2 cut(s) 1110, 1477
PfeI GAWTC 4 cut(s) 68, 236, 269, 1531
PflMI CCANNNNNTGG 1 cut(s) 571
PkrI GCNGC 5 cut(s) 437, 599, 701, 730, 1517
PleI GAGTC 3 cut(s) 13, 370, 1126
PpsI GAGTC 3 cut(s) 13, 370, 1126
PpuMI RGGWCCY 2 cut(s) 445, 1310
PsiI TTATAA 1 cut(s) 1035
Psp5II RGGWCCY 2 cut(s) 445, 1310
Psp6I CCWGG 2 cut(s) 423, 709
PspFI CCCAGC 1 cut(s) 1235
PspGI CCWGG 2 cut(s) 423, 709
PspN4I GGNNCC 6 cut(s) 208, 563, 855, 1270, 1311, 1312
PspPI GGNCC 2 cut(s) 445, 1310
PspPPI RGGWCCY 2 cut(s) 445, 1310
PstI CTGCAG 1 cut(s) 1488
PvuII CAGCTG 2 cut(s) 600, 728
RsaI GTAC 3 cut(s) 124, 193, 404
RsaNI GTAC 3 cut(s) 123, 192, 403
RseI CAYNNNNRTG 1 cut(s) 933
SaqAI TTAA 2 cut(s) 651, 1010
SatI GCNGC 5 cut(s) 436, 598, 700, 729, 1516
Sau3AI GATC 2 cut(s) 1373, 1468
Sau96I GGNCC 2 cut(s) 445, 1310
SbfI CCTGCAGG 1 cut(s) 1488
SchI GAGTC 3 cut(s) 14, 371, 1127
ScrFI CCNGG 2 cut(s) 425, 711
SdaI CCTGCAGG 1 cut(s) 1488
SduI GDGCHC 1 cut(s) 1132
SfaNI GCATC 3 cut(s) 301, 763, 1327
SfcI CTRYAG 1 cut(s) 1484
SinI GGWCC 2 cut(s) 445, 1310
SmiMI CAYNNNNRTG 1 cut(s) 933
SmlI CTYRAG 1 cut(s) 645
SmoI CTYRAG 1 cut(s) 645
Sse8387I CCTGCAGG 1 cut(s) 1488
Sse9I AATT 7 cut(s) 149, 578, 781, 1012, 1100, 1182, 1292
SseBI AGGCCT 1 cut(s) 841
SsiI CCGC 1 cut(s) 507
SspI AATATT 1 cut(s) 655
SspMI CTAG 4 cut(s) 78, 308, 759, 954
StuI AGGCCT 1 cut(s) 841
StyD4I CCNGG 2 cut(s) 423, 709
StyI CCWWGG 1 cut(s) 1369
TaaI ACNGT 4 cut(s) 55, 1093, 1380, 1426
TaiI ACGT 1 cut(s) 1027
TaqI TCGA 1 cut(s) 500
TasI AATT 7 cut(s) 149, 578, 781, 1012, 1100, 1182, 1292
TatI WGTACW 3 cut(s) 122, 191, 402
TfiI GAWTC 4 cut(s) 68, 236, 269, 1531
Tru1I TTAA 2 cut(s) 651, 1010
Tru9I TTAA 2 cut(s) 651, 1010
TscAI CASTG 1 cut(s) 976
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 5 cut(s) 435, 597, 699, 728, 1515
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 6 cut(s) 63, 249, 345, 392, 419, 647
TspRI CASTG 1 cut(s) 976
Van91I CCANNNNNTGG 1 cut(s) 571
VpaK11BI GGWCC 2 cut(s) 445, 1310
XagI CCTNNNNNAGG 1 cut(s) 846
XapI RAATTY 4 cut(s) 578, 1012, 1182, 1292
XspI CTAG 4 cut(s) 78, 308, 759, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.