Rroxscaffold_3G00270430

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003696
Physical Location & Seq
Forward (+)
63001629 .. 63005460
3832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270430.1

Sequence Viewer

Length: 1530 bp
ATGGAGTCTTGGAGCTGTATTTCTGAAGGGAAGGGTTTTGTACCTGATGAAACAATTTCTCATACTGATTCACTTGCTAGAAATAGAAGTGCTTTGATGGGTTGGGATTTGAAAATCCCATGTACTTTTGAAAGTCAAGGTTTTGGGGAATTGGGTTTGGCAGAAATGTTGGGCAAACATCTACCAAGAAGTACAACAAGTATGGGTACCCTAAATCCATTTTTTGGAGAAGGAGAAGATGAATCCTCTTCAAAGCTTTCAAGCTCTATTGTTGAATCCAACAGCAGGGATTCTTCACTCTTTGATTTAAAACTAGATGCTTATACGTCTAAGTCATCAAAAAAAGCTTCGATTTTTTCTACATCCGAGTCATCTACACCTGCAAAGAGAATGAGGATATCAGGAGTGTACTCCCCGACTGCTTATTGCCAGGTTTATGGCTGCAACAAGGACCTCAGCTCCTCTAAGGACTACCACAAGAGGCATAAAGTTTGTGAGGCTCACTCGAAGACCGCCAAAGTCATTGTTAAAGGCATAGAACAGAGGTTTTGTCAGCAATGTAGCAGGTTCCATTTGCTGGCTGAATTTGATGATGGTAAGCGCAGCTGTCGAAAACGCCTTGCCGGACACAATGAGCGCCGGAGAAAGCCTCAAGTTCGTATTCATTCTGGAAGAGATGGGAGGTTGCTTCAGTCATATAGTGGCAGCAGTTTTCCGGGGACTATGTTCACAGCTGCGTCTTTCGTCTGCCAAGATATACTCCCTAGTAGCATTTTAGATGCTGAGAATTACAGAACAAGTGACTGCAGGCGTATAAAAGTGGCAGGTGGCACTCAGCATAGACCTTTGTCAGGAGTGCCTGTTACAAATGGGCATCTGCACTTGAAACCGGTCTTCTCTTCTTATGACAATGGAAAACAGTATCCCCCAGTTCATGTAAATGAAACTAATAATCTTCCTAGTGGAAGCATACTCGCTGAGACTGGTAGTCAGTATCTACATGAGTTGAGAAGCTTAAATTCTGGTTCATGTTCTATTTATCATGAGGTTGCATTAGGAAGTGAAGACTTCAATGATCTCCACACATCAGCATCAACCATGCAAGGATTATCGCGCATTCCAGACTCCGGTTGTGCTCTCTCTCTTCTGTCATCTCAATCACAGAACTCTACAAGCCATTCTTCAGGAATTCCTATTGCTCGTCCCTTGGTAATGCCTGTCAGCCATACACATTACAACCTCAGACAAGTTTCTGATAAGCTTTCCTCACAGGCTTCCACAAGTGGGGTGTTGAATAAATTTCCTTCTTCGGGTCCCATGATGATATCTACTGGAAGTGATGCTGATAATTTTGGAGTCTCAAATGGGATTTTCCAAGGATCACAGTTTTTGAACCCCAAAGATCGCCTATCATGTGATGGTGGGACTACAATTGACTTGCTTCAACTTTCATCAGAGCTCCAACGAGTTGAGGATCAACGGCAATCCCTGCAGGTGAAGAAGGAAAATGACGGTTTCTGCTGCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

509

Amino Acids

55.63

Weight (kDa)

8.42

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 143 - 216 1.6e-32 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 388, 815, 1483
Acc36I ACCTGC 4 cut(s) 388, 555, 815, 1483
Acc65I GGTACC 1 cut(s) 206
AccB1I GGYRCC 1 cut(s) 206
AccB7I CCANNNNNTGG 2 cut(s) 224, 577
AccII CGCG 1 cut(s) 1114
AciI CCGC 1 cut(s) 513
AclWI GGATC 2 cut(s) 1387, 1482
AcsI RAATTY 4 cut(s) 584, 1018, 1188, 1298
AcuI CTGAAG 3 cut(s) 45, 674, 1167
AfaI GTAC 5 cut(s) 42, 124, 193, 208, 410
AfiI CCNNNNNNNGG 7 cut(s) 224, 285, 577, 851, 1127, 1284, 1310
AgeI ACCGGT 1 cut(s) 889
AhdI GACNNNNNGTC 1 cut(s) 987
AjnI CCWGG 1 cut(s) 429
Alw21I GWGCWC 2 cut(s) 1138, 1461
Alw26I GTCTC 2 cut(s) 974, 1363
AlwI GGATC 2 cut(s) 1387, 1482
ApeKI GCWGC 5 cut(s) 441, 603, 705, 734, 1521
ApoI RAATTY 4 cut(s) 584, 1018, 1188, 1298
AsiGI ACCGGT 1 cut(s) 889
Asp718I GGTACC 1 cut(s) 206
AspLEI GCGC 3 cut(s) 603, 639, 1116
AspS9I GGNCC 2 cut(s) 451, 1313
AsuC2I CCSGG 1 cut(s) 717
AsuHPI GGTGA 1 cut(s) 1507
AvaII GGWCC 2 cut(s) 451, 1313
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 1461
BbsI GAAGAC 3 cut(s) 515, 886, 1071
Bbv12I GWGCWC 2 cut(s) 1138, 1461
BbvCI CCTCAGC 1 cut(s) 455
BbvI GCAGC 5 cut(s) 428, 615, 717, 721, 1508
BccI CCATC 4 cut(s) 91, 587, 671, 1412
BceAI ACGGC 1 cut(s) 1496
BciT130I CCWGG 1 cut(s) 431
BciVI GTATCC 1 cut(s) 933
BcnI CCSGG 1 cut(s) 717
BcoDI GTCTC 2 cut(s) 974, 1363
BfaI CTAG 4 cut(s) 78, 314, 765, 960
BfmI CTRYAG 2 cut(s) 805, 1490
BfoI RGCGCY 1 cut(s) 640
BfuAI ACCTGC 4 cut(s) 388, 555, 815, 1483
BfuI GTATCC 1 cut(s) 933
BisI GCNGC 5 cut(s) 442, 604, 706, 735, 1522
BlsI GCNGC 5 cut(s) 443, 605, 707, 736, 1523
Bme1390I CCNGG 2 cut(s) 431, 717
Bme18I GGWCC 2 cut(s) 451, 1313
BmeRI GACNNNNNGTC 1 cut(s) 987
BmgT120I GGNCC 2 cut(s) 451, 1313
BmiI GGNNCC 4 cut(s) 208, 569, 1314, 1315
BmrFI CCNGG 2 cut(s) 431, 717
BmrI ACTGGG 1 cut(s) 923
BmsI GCATC 5 cut(s) 307, 769, 883, 1100, 1330
BmuI ACTGGG 1 cut(s) 923
BoxI GACNNNNGTC 1 cut(s) 847
BpiI GAAGAC 3 cut(s) 515, 886, 1071
BplI GAGNNNNNCTC 4 cut(s) 488, 520, 634, 666
Bpu10I CCTNAGC 1 cut(s) 455
BpuEI CTTGAG 1 cut(s) 636
BpuMI CCSGG 1 cut(s) 717
BsaJI CCNNGG 3 cut(s) 716, 1206, 1375
BsaWI WCCGGW 2 cut(s) 889, 1127
BsaXI ACNNNNNCTCC 4 cut(s) 443, 473, 846, 876
Bsc4I CCNNNNNNNGG 7 cut(s) 224, 285, 577, 851, 1127, 1284, 1310
Bse118I RCCGGY 1 cut(s) 889
Bse1I ACTGG 3 cut(s) 929, 988, 1336
Bse3DI GCAATG 1 cut(s) 563
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 3 cut(s) 716, 1206, 1375
BseGI GGATG 1 cut(s) 362
BseLI CCNNNNNNNGG 7 cut(s) 224, 285, 577, 851, 1127, 1284, 1310
BseMI GCAATG 1 cut(s) 563
BseMII CTCAG 5 cut(s) 469, 774, 848, 969, 1255
BseNI ACTGG 3 cut(s) 929, 988, 1336
BseRI GAGGAG 1 cut(s) 451
BseXI GCAGC 5 cut(s) 428, 615, 717, 721, 1508
BsgI GTGCAG 1 cut(s) 863
Bsh1236I CGCG 1 cut(s) 1114
BshNI GGYRCC 1 cut(s) 206
BshTI ACCGGT 1 cut(s) 889
BsiHKAI GWGCWC 2 cut(s) 1138, 1461
BsiSI CCGG 5 cut(s) 624, 640, 716, 890, 1128
BslFI GGGAC 4 cut(s) 733, 1188, 1299, 1438
BslI CCNNNNNNNGG 7 cut(s) 224, 285, 577, 851, 1127, 1284, 1310
BsmAI GTCTC 2 cut(s) 974, 1363
BsmFI GGGAC 4 cut(s) 733, 1188, 1299, 1438
BsmI GAATGC 1 cut(s) 1116
Bsp1286I GDGCHC 2 cut(s) 1138, 1461
Bsp143I GATC 4 cut(s) 1075, 1379, 1402, 1474
BspACI CCGC 1 cut(s) 513
BspCNI CTCAG 5 cut(s) 468, 775, 847, 970, 1254
BspFNI CGCG 1 cut(s) 1114
BspHI TCATGA 1 cut(s) 1042
BspLI GGNNCC 4 cut(s) 208, 569, 1314, 1315
BspMAI CTGCAG 2 cut(s) 809, 1494
BspMI ACCTGC 4 cut(s) 388, 555, 815, 1483
BspPI GGATC 2 cut(s) 1387, 1482
BspT107I GGYRCC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 563
BsrFI RCCGGY 1 cut(s) 889
BsrI ACTGG 3 cut(s) 929, 988, 1336
BssAI RCCGGY 1 cut(s) 889
BssECI CCNNGG 3 cut(s) 716, 1206, 1375
BssMI GATC 4 cut(s) 1075, 1379, 1402, 1474
BssT1I CCWWGG 2 cut(s) 1206, 1375
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 3 cut(s) 921, 1386, 1514
Bst6I CTCTTC 4 cut(s) 253, 667, 904, 1149
BstAPI GCANNNNNTGC 1 cut(s) 1489
BstC8I GCNNGC 2 cut(s) 579, 809
BstDEI CTNAG 7 cut(s) 330, 455, 465, 783, 834, 978, 1241
BstENI CCTNNNNNAGG 1 cut(s) 849
BstF5I GGATG 1 cut(s) 362
BstFNI CGCG 1 cut(s) 1114
BstH2I RGCGCY 1 cut(s) 640
BstHHI GCGC 3 cut(s) 603, 639, 1116
BstKTI GATC 4 cut(s) 1078, 1382, 1405, 1477
BstMAI GTCTC 2 cut(s) 974, 1363
BstMBI GATC 4 cut(s) 1075, 1379, 1402, 1474
BstMWI GCNNNNNNNGC 1 cut(s) 1489
BstNI CCWGG 1 cut(s) 431
BstPAI GACNNNNGTC 1 cut(s) 847
BstSCI CCNGG 2 cut(s) 429, 715
BstSFI CTRYAG 2 cut(s) 805, 1490
BstUI CGCG 1 cut(s) 1114
BstV1I GCAGC 5 cut(s) 428, 615, 717, 721, 1508
BstV2I GAAGAC 3 cut(s) 515, 886, 1071
BstXI CCANNNNNNTGG 1 cut(s) 437
BsuI GTATCC 1 cut(s) 933
BtsCI GGATG 1 cut(s) 362
BveI ACCTGC 4 cut(s) 388, 555, 815, 1483
Cac8I GCNNGC 2 cut(s) 579, 809
CciI TCATGA 1 cut(s) 1042
CfoI GCGC 3 cut(s) 603, 639, 1116
Cfr10I RCCGGY 1 cut(s) 889
Cfr13I GGNCC 2 cut(s) 451, 1313
CseI GACGC 1 cut(s) 726
Csp6I GTAC 5 cut(s) 41, 123, 192, 207, 409
CspAI ACCGGT 1 cut(s) 889
CviAII CATG 8 cut(s) 120, 935, 1001, 1029, 1043, 1099, 1318, 1413
CviQI GTAC 5 cut(s) 41, 123, 192, 207, 409
DdeI CTNAG 7 cut(s) 330, 455, 465, 783, 834, 978, 1241
DpnI GATC 4 cut(s) 1077, 1381, 1404, 1476
DpnII GATC 4 cut(s) 1075, 1379, 1402, 1474
DraI TTTAAA 1 cut(s) 309
DriI GACNNNNNGTC 1 cut(s) 987
Eam1104I CTCTTC 4 cut(s) 253, 667, 904, 1149
Eam1105I GACNNNNNGTC 1 cut(s) 987
EarI CTCTTC 4 cut(s) 253, 667, 904, 1149
Ecl136II GAGCTC 1 cut(s) 1459
Eco130I CCWWGG 2 cut(s) 1206, 1375
Eco24I GRGCYC 1 cut(s) 1461
Eco32I GATATC 2 cut(s) 399, 1326
Eco47I GGWCC 2 cut(s) 451, 1313
Eco53kI GAGCTC 1 cut(s) 1459
Eco57I CTGAAG 3 cut(s) 45, 674, 1167
EcoICRI GAGCTC 1 cut(s) 1459
EcoNI CCTNNNNNAGG 1 cut(s) 849
EcoO109I RGGNCCY 2 cut(s) 451, 1313
EcoRI GAATTC 1 cut(s) 1188
EcoRII CCWGG 1 cut(s) 429
EcoRV GATATC 2 cut(s) 399, 1326
EcoT14I CCWWGG 2 cut(s) 1206, 1375
EcoT38I GRGCYC 1 cut(s) 1461
ErhI CCWWGG 2 cut(s) 1206, 1375
FaeI CATG 8 cut(s) 123, 938, 1004, 1032, 1046, 1102, 1321, 1416
FalI AAGNNNNNCTT 2 cut(s) 1165, 1197
FaqI GGGAC 4 cut(s) 733, 1188, 1299, 1438
FatI CATG 8 cut(s) 119, 934, 1000, 1028, 1042, 1098, 1317, 1412
Fnu4HI GCNGC 5 cut(s) 442, 604, 706, 735, 1522
FokI GGATG 1 cut(s) 349
FriOI GRGCYC 1 cut(s) 1461
Fsp4HI GCNGC 5 cut(s) 442, 604, 706, 735, 1522
FspBI CTAG 4 cut(s) 78, 314, 765, 960
GlaI GCGC 3 cut(s) 602, 638, 1115
GluI GCNGC 5 cut(s) 442, 604, 706, 735, 1522
HaeII RGCGCY 1 cut(s) 640
HapII CCGG 5 cut(s) 624, 640, 716, 890, 1128
HgaI GACGC 1 cut(s) 726
HhaI GCGC 3 cut(s) 603, 639, 1116
Hin1II CATG 8 cut(s) 123, 938, 1004, 1032, 1046, 1102, 1321, 1416
Hin6I GCGC 3 cut(s) 601, 637, 1114
HinP1I GCGC 3 cut(s) 601, 637, 1114
HindIII AAGCTT 4 cut(s) 254, 345, 1012, 1259
HinfI GANTC 8 cut(s) 5, 68, 242, 275, 290, 368, 1124, 1356
HpaII CCGG 5 cut(s) 624, 640, 716, 890, 1128
HphI GGTGA 1 cut(s) 1507
Hpy166II GTNNAC 2 cut(s) 409, 729
Hpy188I TCNGA 6 cut(s) 25, 367, 1244, 1255, 1456, 1529
Hpy188III TCNNGA 6 cut(s) 402, 669, 852, 1043, 1121, 1185
Hpy8I GTNNAC 2 cut(s) 409, 729
HpyAV CCTTC 5 cut(s) 20, 25, 224, 1314, 1495
HpyCH4III ACNGT 3 cut(s) 921, 1386, 1514
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 7 cut(s) 383, 444, 807, 880, 1052, 1102, 1492
HpyF10VI GCNNNNNNNGC 1 cut(s) 1489
HpyF3I CTNAG 7 cut(s) 330, 455, 465, 783, 834, 978, 1241
HpySE526I ACGT 1 cut(s) 326
Hsp92II CATG 8 cut(s) 123, 938, 1004, 1032, 1046, 1102, 1321, 1416
HspAI GCGC 3 cut(s) 601, 637, 1114
KflI GGGWCCC 1 cut(s) 1313
KpnI GGTACC 1 cut(s) 210
Kzo9I GATC 4 cut(s) 1075, 1379, 1402, 1474
LmnI GCTCC 3 cut(s) 12, 464, 1464
Lsp1109I GCAGC 5 cut(s) 428, 615, 717, 721, 1508
LweI GCATC 5 cut(s) 307, 769, 883, 1100, 1330
MaeI CTAG 4 cut(s) 78, 314, 765, 960
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 2 cut(s) 800, 862
MalI GATC 4 cut(s) 1077, 1381, 1404, 1476
MboI GATC 4 cut(s) 1075, 1379, 1402, 1474
MfeI CAATTG 1 cut(s) 1431
MhlI GDGCHC 2 cut(s) 1138, 1461
MluCI AATT 9 cut(s) 54, 149, 584, 787, 1018, 1188, 1298, 1348, 1431
MlyI GAGTC 4 cut(s) 14, 377, 1118, 1365
MmeI TCCRAC 2 cut(s) 303, 1486
MseI TTAA 3 cut(s) 308, 528, 1016
MslI CAYNNNNRTG 1 cut(s) 939
MspA1I CMGCKG 2 cut(s) 606, 734
MspI CCGG 5 cut(s) 624, 640, 716, 890, 1128
MspR9I CCNGG 2 cut(s) 431, 717
MunI CAATTG 1 cut(s) 1431
Mva1269I GAATGC 1 cut(s) 1116
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 1 cut(s) 1114
MwoI GCNNNNNNNGC 1 cut(s) 1489
NciI CCSGG 1 cut(s) 717
NdeII GATC 4 cut(s) 1075, 1379, 1402, 1474
NlaIII CATG 8 cut(s) 123, 938, 1004, 1032, 1046, 1102, 1321, 1416
NlaIV GGNNCC 4 cut(s) 208, 569, 1314, 1315
NmuCI GTSAC 1 cut(s) 800
PagI TCATGA 1 cut(s) 1042
PaqCI CACCTGC 3 cut(s) 388, 815, 1483
PctI GAATGC 1 cut(s) 1116
PfeI GAWTC 4 cut(s) 68, 242, 275, 290
PflMI CCANNNNNTGG 2 cut(s) 224, 577
PinAI ACCGGT 1 cut(s) 889
PkrI GCNGC 5 cut(s) 443, 605, 707, 736, 1523
PleI GAGTC 4 cut(s) 13, 376, 1118, 1364
PpsI GAGTC 4 cut(s) 13, 376, 1118, 1364
PpuMI RGGWCCY 2 cut(s) 451, 1313
PshAI GACNNNNGTC 1 cut(s) 847
Psp124BI GAGCTC 1 cut(s) 1461
Psp5II RGGWCCY 2 cut(s) 451, 1313
Psp6I CCWGG 1 cut(s) 429
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 4 cut(s) 208, 569, 1314, 1315
PspPI GGNCC 2 cut(s) 451, 1313
PspPPI RGGWCCY 2 cut(s) 451, 1313
PstI CTGCAG 2 cut(s) 809, 1494
PvuII CAGCTG 2 cut(s) 606, 734
RsaI GTAC 5 cut(s) 42, 124, 193, 208, 410
RsaNI GTAC 5 cut(s) 41, 123, 192, 207, 409
RseI CAYNNNNRTG 1 cut(s) 939
SacI GAGCTC 1 cut(s) 1461
SaqAI TTAA 3 cut(s) 308, 528, 1016
SatI GCNGC 5 cut(s) 442, 604, 706, 735, 1522
Sau3AI GATC 4 cut(s) 1075, 1379, 1402, 1474
Sau96I GGNCC 2 cut(s) 451, 1313
SbfI CCTGCAGG 1 cut(s) 1494
SchI GAGTC 4 cut(s) 14, 377, 1118, 1365
ScrFI CCNGG 2 cut(s) 431, 717
SdaI CCTGCAGG 1 cut(s) 1494
SduI GDGCHC 2 cut(s) 1138, 1461
SfaNI GCATC 5 cut(s) 307, 769, 883, 1100, 1330
SfcI CTRYAG 2 cut(s) 805, 1490
SinI GGWCC 2 cut(s) 451, 1313
SmiMI CAYNNNNRTG 1 cut(s) 939
SmlI CTYRAG 1 cut(s) 651
SmoI CTYRAG 1 cut(s) 651
Sse8387I CCTGCAGG 1 cut(s) 1494
Sse9I AATT 9 cut(s) 54, 149, 584, 787, 1018, 1188, 1298, 1348, 1431
SsiI CCGC 1 cut(s) 513
SspMI CTAG 4 cut(s) 78, 314, 765, 960
SstI GAGCTC 1 cut(s) 1461
StyD4I CCNGG 2 cut(s) 429, 715
StyI CCWWGG 2 cut(s) 1206, 1375
TaaI ACNGT 3 cut(s) 921, 1386, 1514
TaiI ACGT 1 cut(s) 329
TaqI TCGA 3 cut(s) 350, 506, 610
TasI AATT 9 cut(s) 54, 149, 584, 787, 1018, 1188, 1298, 1348, 1431
TatI WGTACW 3 cut(s) 122, 191, 408
TfiI GAWTC 4 cut(s) 68, 242, 275, 290
Tru1I TTAA 3 cut(s) 308, 528, 1016
Tru9I TTAA 3 cut(s) 308, 528, 1016
TseFI GTSAC 1 cut(s) 800
TseI GCWGC 5 cut(s) 441, 603, 705, 734, 1521
Tsp45I GTSAC 1 cut(s) 800
TspDTI ATGAA 7 cut(s) 63, 255, 653, 923, 957, 1017, 1440
Van91I CCANNNNNTGG 2 cut(s) 224, 577
VpaK11BI GGWCC 2 cut(s) 451, 1313
XagI CCTNNNNNAGG 1 cut(s) 849
XapI RAATTY 4 cut(s) 584, 1018, 1188, 1298
XspI CTAG 4 cut(s) 78, 314, 765, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.