MD06G1204000.v1.1

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003696
Physical Location & Seq
Reverse (-)
33858806 .. 33862524
3719 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1204000.v1.1.491

Sequence Viewer

Length: 1473 bp
ATGGGTTGGGAACTGAAAATCCCATGTACCTTCATGGAAAGTCAAGGTTTTGGGGAGTTGGGTAACTTGGGTTTGCCAGAAATGGTGGGCAAACATTTGCAGAGTGATTCAATTAGAGATGTAGTGAGCACAAGTATGTCTACCCCGAATTTGTTTTCGGGTGAAGCCGAATCCTTTTCGAGGATTTCGAGCTCCATTGTGGACTCCAGCAGTAGGGATTCTTCACTCATTGATTTAAAACTAGGGAGATTGGCTGATCACAAAGGTGTTCATAGCTCTAAATTGTCTAAAGGAACTCCCATGTTGTCCTCATCTGAATCATCCACACCTACGAAGAGAATGCGTGTTTCGGGTGTGTATTCTCAGCCCGCCTACTGCCAGGTTTATGGTTGCAACAAGGATCTTAGCTCCTGTAAGGACTACCACAAGAGGCATAAAGTCTGCGAGGTTCACTCGAAGACTGCGAAAGTTATTATCAATGGCATAGAACAAAGGTTTTGTCAACAATGTAGCAGGTTTCATTTGTTGGGTGAATTTGATGATGGTAAGCGTAGCTGTCGTAAGCGCCTTGCAGGACACAACGAGCGGCGGAGAAAGCCTCAGATTGGGAGTCATTCTGGAAGAGCTGAGAGGTTCCCTCAGTCATTTGATGGTGGCAGATTTGAAGGGAATATGTTAACTACTGCATCTTTTATCTGCCAAGAGATACTCCCTAGTGGCAATTTGAATCCTGAGAAATATGGAACAAGTGACTGGTGCAGGCGCCTAAAAGTTGAAGGCGGGACTCATTATGGGCCTTTTTTGGGGATGCCTGTTGCAAACGGGCATCTGCATTCGAAACCTGTCCTCCCTTCTTACGATATTGGCAAACAGTATCCCCCATTTCATGAAAACGGAACTAATCCTCAAAATGCAAGCATATTTGGTCAGAACGGTAGTCAATATCCACAAGATGCGAGAGGCCTAAGTTCCGGTTCACATTCTATTTTTCATGAGACCGCATTAGGAAGTCAAAACTTCAATGTTTTTGACACTGCTTCAGCAGTTCAAGGATTATTGGGAATCTCAGACTCTGGCTGTGCTCTCTCTCTTCTGTCATCTCAATCACAGAACTCTTCAGGCTATTTGTCAGGAATTCCTATTGCGCGTCCCTTAGTCATACCAGCCAGCCATAACTTCCCTTCTTCAGGAATGAATTCTGGAGCTGGCAATCACCTGAGTCCCATGCCGCTATCTAATGGTTGTGATGATACCGATGATTTTGGAATCTCAGATGGGATTTTCCAAGGATCAGGTTTTGTGAACACAAAAGATCGTCTACCCTTTCAAGATGGAGCCACTATCAACTTGCTTCAACTGTCATCGCAGCTCCAACGAGTAGAGGATCAACGGCAATCCATGCTGGTGAAGCAGGAAAATGATGCTTTCTGCTTTACTGAGAATCACTTAAAAAGTGATCTGCAAATAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

491

Amino Acids

53.47

Weight (kDa)

7.93

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 126 - 199 9.6e-32 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 504
AccB1I GGYRCC 1 cut(s) 762
AccBSI CCGCTC 1 cut(s) 586
AccI GTMKAC 2 cut(s) 140, 1318
AccII CGCG 1 cut(s) 1147
AciI CCGC 6 cut(s) 369, 586, 589, 780, 999, 1229
AclWI GGATC 3 cut(s) 408, 1297, 1392
AcsI RAATTY 4 cut(s) 148, 533, 1134, 1195
AcuI CTGAAG 3 cut(s) 1023, 1101, 1170
AcyI GRCGYC 1 cut(s) 763
AfaI GTAC 1 cut(s) 28
AfiI CCNNNNNNNGG 5 cut(s) 180, 213, 605, 803, 1187
AgsI TTSAA 8 cut(s) 111, 665, 727, 776, 1021, 1049, 1328, 1355
AjnI CCWGG 1 cut(s) 378
AleI CACNNNNGTG 1 cut(s) 264
AloI GAACNNNNNNTCC 1 cut(s) 35
AluBI AGCT 7 cut(s) 192, 276, 408, 555, 626, 1205, 1369
AluI AGCT 7 cut(s) 192, 276, 408, 555, 626, 1205, 1369
Alw21I GWGCWC 3 cut(s) 131, 194, 1084
Alw26I GTCTC 1 cut(s) 989
AlwI GGATC 3 cut(s) 408, 1297, 1392
AlwNI CAGNNNCTG 1 cut(s) 1073
AoxI GGCC 2 cut(s) 794, 961
ApeKI GCWGC 1 cut(s) 1366
ApoI RAATTY 4 cut(s) 148, 533, 1134, 1195
ArsI GACNNNNNNTTYG 2 cut(s) 484, 516
Asp700I GAANNNNTTC 1 cut(s) 1195
AspLEI GCGC 3 cut(s) 567, 765, 1147
AspS9I GGNCC 1 cut(s) 794
AsuHPI GGTGA 4 cut(s) 173, 542, 1205, 1417
AsuII TTCGAA 1 cut(s) 836
BanI GGYRCC 1 cut(s) 762
BanII GRGCYC 1 cut(s) 194
BarI GAAGNNNNNNTAC 2 cut(s) 205, 237
BbsI GAAGAC 1 cut(s) 464
Bbv12I GWGCWC 3 cut(s) 131, 194, 1084
BbvI GCAGC 1 cut(s) 1378
BccI CCATC 4 cut(s) 536, 644, 1268, 1325
BceAI ACGGC 1 cut(s) 1406
BcgI CGANNNNNNTGC 2 cut(s) 322, 356
BciT130I CCWGG 1 cut(s) 380
BciVI GTATCC 1 cut(s) 885
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 989
BfaI CTAG 2 cut(s) 242, 714
BfoI RGCGCY 2 cut(s) 568, 766
BfuAI ACCTGC 1 cut(s) 504
BfuI GTATCC 1 cut(s) 885
BisI GCNGC 3 cut(s) 587, 1229, 1367
BlsI GCNGC 3 cut(s) 588, 1230, 1368
Bme1390I CCNGG 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 794
BmiI GGNNCC 3 cut(s) 635, 764, 1336
BmrFI CCNGG 1 cut(s) 380
BmsI GCATC 5 cut(s) 695, 798, 835, 943, 1411
BpiI GAAGAC 1 cut(s) 464
BplI GAGNNNNNCTC 6 cut(s) 437, 469, 583, 615, 622, 654
BpmI CTGGAG 2 cut(s) 190, 1221
Bpu14I TTCGAA 1 cut(s) 836
BsaHI GRCGYC 1 cut(s) 763
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 1 cut(s) 1285
BsaWI WCCGGW 1 cut(s) 971
BsaXI ACNNNNNCTCC 2 cut(s) 831, 861
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 213, 605, 803, 1187
Bse1I ACTGG 1 cut(s) 758
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 1 cut(s) 1285
BseGI GGATG 2 cut(s) 320, 813
BseLI CCNNNNNNNGG 5 cut(s) 180, 213, 605, 803, 1187
BseMII CTCAG 9 cut(s) 377, 614, 618, 653, 723, 1080, 1208, 1284, 1428
BseNI ACTGG 1 cut(s) 758
BseXI GCAGC 1 cut(s) 1378
BsgI GTGCAG 1 cut(s) 778
Bsh1236I CGCG 1 cut(s) 1147
BshFI GGCC 2 cut(s) 796, 963
BshNI GGYRCC 1 cut(s) 762
BsiHKAI GWGCWC 3 cut(s) 131, 194, 1084
BsiSI CCGG 1 cut(s) 972
BslFI GGGAC 3 cut(s) 796, 1134, 1206
BslI CCNNNNNNNGG 5 cut(s) 180, 213, 605, 803, 1187
BsmAI GTCTC 1 cut(s) 989
BsmFI GGGAC 3 cut(s) 796, 1134, 1206
BsmI GAATGC 2 cut(s) 345, 832
BsnI GGCC 2 cut(s) 796, 963
Bso31I GGTCTC 1 cut(s) 989
Bsp119I TTCGAA 1 cut(s) 836
Bsp1286I GDGCHC 3 cut(s) 131, 194, 1084
Bsp143I GATC 6 cut(s) 256, 400, 1289, 1312, 1384, 1456
BspACI CCGC 6 cut(s) 369, 586, 589, 780, 999, 1229
BspANI GGCC 2 cut(s) 796, 963
BspCNI CTCAG 9 cut(s) 376, 613, 619, 652, 724, 1079, 1209, 1283, 1429
BspFNI CGCG 1 cut(s) 1147
BspHI TCATGA 2 cut(s) 886, 991
BspLI GGNNCC 3 cut(s) 635, 764, 1336
BspMI ACCTGC 1 cut(s) 504
BspPI GGATC 3 cut(s) 408, 1297, 1392
BspQI GCTCTTC 1 cut(s) 616
BspT104I TTCGAA 1 cut(s) 836
BspT107I GGYRCC 1 cut(s) 762
BspTNI GGTCTC 1 cut(s) 989
BsrBI CCGCTC 1 cut(s) 586
BsrI ACTGG 1 cut(s) 758
BssECI CCNNGG 1 cut(s) 1285
BssMI GATC 6 cut(s) 256, 400, 1289, 1312, 1384, 1456
BssNI GRCGYC 1 cut(s) 763
BssT1I CCWWGG 1 cut(s) 1285
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 3 cut(s) 873, 935, 1359
Bst6I CTCTTC 4 cut(s) 329, 616, 1095, 1120
BstACI GRCGYC 1 cut(s) 763
BstAPI GCANNNNNTGC 1 cut(s) 1399
BstBI TTCGAA 1 cut(s) 836
BstC8I GCNNGC 5 cut(s) 369, 761, 916, 1168, 1207
BstENI CCTNNNNNAGG 2 cut(s) 178, 1185
BstF5I GGATG 2 cut(s) 320, 813
BstFNI CGCG 1 cut(s) 1147
BstH2I RGCGCY 2 cut(s) 568, 766
BstHHI GCGC 3 cut(s) 567, 765, 1147
BstKTI GATC 6 cut(s) 259, 403, 1292, 1315, 1387, 1459
BstMAI GTCTC 1 cut(s) 989
BstMBI GATC 6 cut(s) 256, 400, 1289, 1312, 1384, 1456
BstMWI GCNNNNNNNGC 3 cut(s) 595, 1399, 1408
BstNI CCWGG 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 378
BstUI CGCG 1 cut(s) 1147
BstV1I GCAGC 1 cut(s) 1378
BstV2I GAAGAC 1 cut(s) 464
BstX2I RGATCY 1 cut(s) 400
BstXI CCANNNNNNTGG 1 cut(s) 386
BstYI RGATCY 1 cut(s) 400
BsuI GTATCC 1 cut(s) 885
BsuRI GGCC 2 cut(s) 796, 963
BtgZI GCGATG 1 cut(s) 1347
BtsCI GGATG 2 cut(s) 320, 813
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 1 cut(s) 1032
BveI ACCTGC 1 cut(s) 504
Cac8I GCNNGC 5 cut(s) 369, 761, 916, 1168, 1207
CaiI CAGNNNCTG 1 cut(s) 1073
CciI TCATGA 2 cut(s) 886, 991
CfoI GCGC 3 cut(s) 567, 765, 1147
Cfr13I GGNCC 1 cut(s) 794
CseI GACGC 1 cut(s) 1136
Csp6I GTAC 1 cut(s) 27
CviAII CATG 7 cut(s) 24, 34, 301, 887, 992, 1225, 1399
CviQI GTAC 1 cut(s) 27
DinI GGCGCC 1 cut(s) 764
DpnI GATC 6 cut(s) 258, 402, 1291, 1314, 1386, 1458
DpnII GATC 6 cut(s) 256, 400, 1289, 1312, 1384, 1456
DraI TTTAAA 1 cut(s) 237
Eam1104I CTCTTC 4 cut(s) 329, 616, 1095, 1120
EarI CTCTTC 4 cut(s) 329, 616, 1095, 1120
EciI GGCGGA 1 cut(s) 604
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 1285
Eco147I AGGCCT 1 cut(s) 963
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 989
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 3 cut(s) 1023, 1101, 1170
EcoICRI GAGCTC 1 cut(s) 192
EcoNI CCTNNNNNAGG 2 cut(s) 178, 1185
EcoRI GAATTC 2 cut(s) 1134, 1195
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 1285
EcoT38I GRGCYC 1 cut(s) 194
EgeI GGCGCC 1 cut(s) 764
EheI GGCGCC 1 cut(s) 764
ErhI CCWWGG 1 cut(s) 1285
FaeI CATG 7 cut(s) 27, 37, 304, 890, 995, 1228, 1402
FaqI GGGAC 3 cut(s) 796, 1134, 1206
FatI CATG 7 cut(s) 23, 33, 300, 886, 991, 1224, 1398
FauI CCCGC 2 cut(s) 376, 773
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 2 cut(s) 140, 1318
Fnu4HI GCNGC 3 cut(s) 587, 1229, 1367
FokI GGATG 2 cut(s) 307, 820
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 3 cut(s) 587, 1229, 1367
FspBI CTAG 2 cut(s) 242, 714
GlaI GCGC 3 cut(s) 566, 764, 1146
GluI GCNGC 3 cut(s) 587, 1229, 1367
GsuI CTGGAG 2 cut(s) 190, 1221
HaeII RGCGCY 2 cut(s) 568, 766
HaeIII GGCC 2 cut(s) 796, 963
HapII CCGG 1 cut(s) 972
HgaI GACGC 1 cut(s) 1136
HhaI GCGC 3 cut(s) 567, 765, 1147
Hin1I GRCGYC 1 cut(s) 763
Hin1II CATG 7 cut(s) 27, 37, 304, 890, 995, 1228, 1402
Hin6I GCGC 3 cut(s) 565, 763, 1145
HinP1I GCGC 3 cut(s) 565, 763, 1145
HincII GTYRAC 2 cut(s) 503, 678
HindII GTYRAC 2 cut(s) 503, 678
HpaI GTTAAC 1 cut(s) 678
HpaII CCGG 1 cut(s) 972
HphI GGTGA 4 cut(s) 173, 542, 1205, 1417
Hpy166II GTNNAC 8 cut(s) 141, 202, 451, 503, 678, 977, 1303, 1319
Hpy188I TCNGA 5 cut(s) 316, 603, 930, 1069, 1273
Hpy188III TCNNGA 8 cut(s) 618, 731, 887, 992, 1131, 1188, 1200, 1328
Hpy8I GTNNAC 8 cut(s) 141, 202, 451, 503, 678, 977, 1303, 1319
HpyAV CCTTC 5 cut(s) 40, 659, 770, 861, 1191
HpyCH4III ACNGT 3 cut(s) 873, 935, 1359
HpyCH4V TGCA 9 cut(s) 100, 393, 572, 686, 759, 818, 832, 914, 1462
HpyF10VI GCNNNNNNNGC 3 cut(s) 595, 1399, 1408
Hsp92I GRCGYC 1 cut(s) 763
Hsp92II CATG 7 cut(s) 27, 37, 304, 890, 995, 1228, 1402
HspAI GCGC 3 cut(s) 565, 763, 1145
KasI GGCGCC 1 cut(s) 762
Ksp22I TGATCA 1 cut(s) 256
KspAI GTTAAC 1 cut(s) 678
Kzo9I GATC 6 cut(s) 256, 400, 1289, 1312, 1384, 1456
LguI GCTCTTC 1 cut(s) 616
LmnI GCTCC 5 cut(s) 197, 413, 1202, 1334, 1374
Lsp1109I GCAGC 1 cut(s) 1378
LweI GCATC 5 cut(s) 695, 798, 835, 943, 1411
MaeI CTAG 2 cut(s) 242, 714
MaeIII GTNAC 2 cut(s) 62, 749
MalI GATC 6 cut(s) 258, 402, 1291, 1314, 1386, 1458
MbiI CCGCTC 1 cut(s) 586
MboI GATC 6 cut(s) 256, 400, 1289, 1312, 1384, 1456
MboII GAAGA 7 cut(s) 213, 346, 469, 633, 1082, 1107, 1176
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 3 cut(s) 131, 194, 1084
MluCI AATT 7 cut(s) 111, 148, 281, 533, 721, 1134, 1195
Mly113I GGCGCC 1 cut(s) 763
MlyI GAGTC 5 cut(s) 197, 619, 778, 1064, 1228
MmeI TCCRAC 1 cut(s) 1396
MroXI GAANNNNTTC 1 cut(s) 1195
MseI TTAA 3 cut(s) 236, 677, 1448
MslI CAYNNNNRTG 3 cut(s) 134, 264, 1403
MspI CCGG 1 cut(s) 972
MspR9I CCNGG 1 cut(s) 380
Mva1269I GAATGC 2 cut(s) 345, 832
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 1 cut(s) 1147
MwoI GCNNNNNNNGC 3 cut(s) 595, 1399, 1408
NarI GGCGCC 1 cut(s) 763
NdeII GATC 6 cut(s) 256, 400, 1289, 1312, 1384, 1456
NlaIII CATG 7 cut(s) 27, 37, 304, 890, 995, 1228, 1402
NlaIV GGNNCC 3 cut(s) 635, 764, 1336
NmuCI GTSAC 1 cut(s) 749
NspV TTCGAA 1 cut(s) 836
OliI CACNNNNGTG 1 cut(s) 264
PagI TCATGA 2 cut(s) 886, 991
PceI AGGCCT 1 cut(s) 963
PciSI GCTCTTC 1 cut(s) 616
PcsI WCGNNNNNNNCGW 2 cut(s) 185, 461
PctI GAATGC 2 cut(s) 345, 832
PdmI GAANNNNTTC 1 cut(s) 1195
PfeI GAWTC 8 cut(s) 107, 170, 218, 317, 727, 1062, 1266, 1441
PkrI GCNGC 3 cut(s) 588, 1230, 1368
PleI GAGTC 5 cut(s) 197, 618, 778, 1064, 1227
PluTI GGCGCC 1 cut(s) 766
PpsI GAGTC 5 cut(s) 197, 618, 778, 1064, 1227
Psp124BI GAGCTC 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 3 cut(s) 635, 764, 1336
PspPI GGNCC 1 cut(s) 794
PstNI CAGNNNCTG 1 cut(s) 1073
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
RseI CAYNNNNRTG 3 cut(s) 134, 264, 1403
SacI GAGCTC 1 cut(s) 194
SapI GCTCTTC 1 cut(s) 616
SaqAI TTAA 3 cut(s) 236, 677, 1448
SatI GCNGC 3 cut(s) 587, 1229, 1367
Sau3AI GATC 6 cut(s) 256, 400, 1289, 1312, 1384, 1456
Sau96I GGNCC 1 cut(s) 794
SchI GAGTC 5 cut(s) 197, 619, 778, 1064, 1228
ScrFI CCNGG 1 cut(s) 380
SduI GDGCHC 3 cut(s) 131, 194, 1084
SfaNI GCATC 5 cut(s) 695, 798, 835, 943, 1411
SfoI GGCGCC 1 cut(s) 764
SfuI TTCGAA 1 cut(s) 836
SmiMI CAYNNNNRTG 3 cut(s) 134, 264, 1403
Sse9I AATT 7 cut(s) 111, 148, 281, 533, 721, 1134, 1195
SseBI AGGCCT 1 cut(s) 963
SsiI CCGC 6 cut(s) 369, 586, 589, 780, 999, 1229
SspDI GGCGCC 1 cut(s) 762
SspMI CTAG 2 cut(s) 242, 714
SstI GAGCTC 1 cut(s) 194
StuI AGGCCT 1 cut(s) 963
StyD4I CCNGG 1 cut(s) 378
StyI CCWWGG 1 cut(s) 1285
TaaI ACNGT 3 cut(s) 873, 935, 1359
TaqI TCGA 4 cut(s) 179, 188, 455, 836
TasI AATT 7 cut(s) 111, 148, 281, 533, 721, 1134, 1195
TauI GCSGC 2 cut(s) 589, 1231
TfiI GAWTC 8 cut(s) 107, 170, 218, 317, 727, 1062, 1266, 1441
Tru1I TTAA 3 cut(s) 236, 677, 1448
Tru9I TTAA 3 cut(s) 236, 677, 1448
TscAI CASTG 1 cut(s) 1039
TseFI GTSAC 1 cut(s) 749
TseI GCWGC 1 cut(s) 1366
Tsp45I GTSAC 1 cut(s) 749
TspDTI ATGAA 7 cut(s) 22, 260, 509, 875, 903, 980, 1208
TspGWI ACGGA 1 cut(s) 909
TspRI CASTG 1 cut(s) 1039
XagI CCTNNNNNAGG 2 cut(s) 178, 1185
XapI RAATTY 4 cut(s) 148, 533, 1134, 1195
XmiI GTMKAC 2 cut(s) 140, 1318
XmnI GAANNNNTTC 1 cut(s) 1195
XspI CTAG 2 cut(s) 242, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.