MD14G1215300.v1.1

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003696
Physical Location & Seq
Reverse (-)
29884331 .. 29887872
3542 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1215300.v1.1.491

Sequence Viewer

Length: 1533 bp
ATGGAATCTTGGAGCTGTATTTCTGAAGGGAAAGGGTTTGTTGTGTCAGATGGAACAATTTCACATACTGATTCACTTTCTAGAAGTAGAAATGGTTTGATGGGTTGGGAATTGAACACCCCTTGTACTTTTATGGAAAGTCAAGGTTTTGGGGAGTTGGGTTTTCCAGAAATGGTGGGCAAACATTTGCAGAATGATTCAATTAGAGATGCAGTGAGCACAAGTATGTCTACCCCGAATTTGTTTTTCGGTGAAGACGAATCCTCTTCGAGGATTTCGAGCTCCATTGTGGACTCCAGCAGTAGGGATTCTTCACTCATTGACTTAAAACTAGGGAGATTGGCTGATCACAGAGATGTTCATAGCTCTAAATTGTCTAAAGGAACTCCCATGTTGTCTTCATCTGGATCATCAACGCCCCCTAAGCGAATGCGCGTTTTGGGTGTGTATCCTCGGCCTGCCTACTGCCAGGTTTATGGTTGCAACAAGGATCTTAGCTCCTATAAGGACTACCACAAGAGGCATAAGGTTTGCGAGGTTCACTCGAAGACTGCGAAAGTTATTGTCAACGGCATAGAACAGAGGTTTTGTCAACAATGTAGCAGGTTTCATTTGTTGGGTGAATTTGATGATGGTAAGCGTAGCTGTCGTAGGCGCCTTGCAGGACACAACGAGCGGCGGAGAAAGCCTCAGATAGGGAGTCATCCTGGAAGAGCTGGGAGGTTCCCTCAGTCATTTGATGGTGGCAGATTTGAAGGGAATATGTTAGCAACTGCATCTTTTATCTGCCAAGAGATACTCCCTACTGACATTCCTGAGAAACATGGAATGGGTGACTGCTGCAGGCACGTAAAATTTGAAGGCGGGACTCATTATAGGCCTTTGTCGGGGATGCCTGTTACAAACGGGCATCTGCATTCAAAACCTGTCTTCCCTTCTTACGATATTGGCAAACAGTATCCCTCATTTCATGAAAACGGAACTAATCCTCTAACTGCAAGCATATTCGGTCAGAATGGTGGTCAATATCCACAAGATGTGAGAGGCCTAAGTTCTGGTTCACATTCTATTTTTCATGAGACCGCATTAGGAAGTCAAGATTTCAATGTTTTCAACACAGCTTCAGCAGTTCCAGGATTATCAGGAATCTCAAACTCTGGCTGTGCTCGCTCTCTTCTGTCATCTCAATCACAGAACTCTTCAAGCTATTTGTCAGGAATTCCTATCGTGCGCCCCTTGGTCATACCAGCCAGCCATAAATTCCCTTCTTCAGGAATGAATTCTGGCTCTAGGAGTCACCTGAGTCCCATACTGCTATCTAATGATTGTGATGATACCAATGATTTTGGAATCTCAGATGGGATTATCCAAGGATCAGGTTTTGCGAACACCAAAGATCGTCTACCCTGTCAAGATGGAGCCACTATCGACTTGCTTCAACTGTCATCGCAGCTCCAACAAGTAGAGGATCAACGGCAATCCATGCAGGCGAAGCAGGAAAATGATGCTTTCTGCTGCCTCAGAATCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

511

Amino Acids

55.69

Weight (kDa)

8.11

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 156 - 229 2.5e-32 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 594
AccB1I GGYRCC 1 cut(s) 654
AccBSI CCGCTC 1 cut(s) 676
AccI GTMKAC 2 cut(s) 230, 1402
AccII CGCG 1 cut(s) 435
AciI CCGC 4 cut(s) 676, 679, 864, 1083
AclWI GGATC 4 cut(s) 415, 498, 1381, 1476
AcsI RAATTY 6 cut(s) 238, 623, 854, 1218, 1259, 1279
AcuI CTGAAG 3 cut(s) 45, 1107, 1254
AcyI GRCGYC 1 cut(s) 655
AfaI GTAC 1 cut(s) 127
AfiI CCNNNNNNNGG 5 cut(s) 270, 303, 695, 887, 1271
AgsI TTSAA 9 cut(s) 115, 201, 755, 860, 921, 1105, 1114, 1203, 1439
AjnI CCWGG 3 cut(s) 468, 706, 1132
AluBI AGCT 9 cut(s) 15, 282, 366, 498, 645, 716, 1121, 1206, 1453
AluI AGCT 9 cut(s) 15, 282, 366, 498, 645, 716, 1121, 1206, 1453
Alw21I GWGCWC 3 cut(s) 221, 284, 1168
Alw26I GTCTC 1 cut(s) 1073
AlwI GGATC 4 cut(s) 415, 498, 1381, 1476
AoxI GGCC 3 cut(s) 455, 878, 1045
ApeKI GCWGC 3 cut(s) 840, 1450, 1515
ApoI RAATTY 6 cut(s) 238, 623, 854, 1218, 1259, 1279
Asp700I GAANNNNTTC 2 cut(s) 58, 1279
AspLEI GCGC 3 cut(s) 435, 657, 1233
AsuHPI GGTGA 4 cut(s) 263, 632, 845, 1289
BanI GGYRCC 1 cut(s) 654
BanII GRGCYC 1 cut(s) 284
BarI GAAGNNNNNNTAC 2 cut(s) 295, 327
BbsI GAAGAC 4 cut(s) 261, 390, 554, 922
Bbv12I GWGCWC 3 cut(s) 221, 284, 1168
BbvI GCAGC 3 cut(s) 827, 1462, 1502
BccI CCATC 6 cut(s) 44, 94, 626, 734, 1352, 1409
BceAI ACGGC 2 cut(s) 586, 1490
BciT130I CCWGG 3 cut(s) 470, 708, 1134
BciVI GTATCC 2 cut(s) 459, 969
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 1073
BfaI CTAG 3 cut(s) 81, 332, 1290
BfmI CTRYAG 1 cut(s) 841
BfoI RGCGCY 1 cut(s) 658
BfuAI ACCTGC 1 cut(s) 594
BfuI GTATCC 2 cut(s) 459, 969
BisI GCNGC 4 cut(s) 677, 841, 1451, 1516
BlsI GCNGC 4 cut(s) 678, 842, 1452, 1517
Bme1390I CCNGG 3 cut(s) 470, 708, 1134
BmiI GGNNCC 3 cut(s) 656, 725, 1420
BmrFI CCNGG 3 cut(s) 470, 708, 1134
BmsI GCATC 5 cut(s) 199, 785, 882, 919, 1495
BpiI GAAGAC 4 cut(s) 261, 390, 554, 922
BplI GAGNNNNNCTC 6 cut(s) 527, 559, 673, 705, 712, 744
BpmI CTGGAG 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 423
BsaAI YACGTR 1 cut(s) 850
BsaHI GRCGYC 1 cut(s) 655
BsaI GGTCTC 1 cut(s) 1073
BsaJI CCNNGG 3 cut(s) 452, 1236, 1369
Bsc4I CCNNNNNNNGG 5 cut(s) 270, 303, 695, 887, 1271
BseBI CCWGG 3 cut(s) 470, 708, 1134
BseDI CCNNGG 3 cut(s) 452, 1236, 1369
BseGI GGATG 2 cut(s) 703, 897
BseLI CCNNNNNNNGG 5 cut(s) 270, 303, 695, 887, 1271
BseMII CTCAG 5 cut(s) 704, 743, 807, 1292, 1368
BseXI GCAGC 3 cut(s) 827, 1462, 1502
BseYI CCCAGC 1 cut(s) 716
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 3 cut(s) 457, 880, 1047
BshNI GGYRCC 1 cut(s) 654
BsiHKAI GWGCWC 3 cut(s) 221, 284, 1168
BslFI GGGAC 2 cut(s) 880, 1290
BslI CCNNNNNNNGG 5 cut(s) 270, 303, 695, 887, 1271
BsmAI GTCTC 1 cut(s) 1073
BsmFI GGGAC 2 cut(s) 880, 1290
BsmI GAATGC 2 cut(s) 435, 916
BsnI GGCC 3 cut(s) 457, 880, 1047
Bso31I GGTCTC 1 cut(s) 1073
Bsp1286I GDGCHC 3 cut(s) 221, 284, 1168
Bsp143I GATC 6 cut(s) 346, 407, 490, 1373, 1396, 1468
BspACI CCGC 4 cut(s) 676, 679, 864, 1083
BspANI GGCC 3 cut(s) 457, 880, 1047
BspCNI CTCAG 6 cut(s) 703, 742, 808, 1293, 1367, 1533
BspFNI CGCG 1 cut(s) 435
BspHI TCATGA 2 cut(s) 970, 1075
BspLI GGNNCC 3 cut(s) 656, 725, 1420
BspMAI CTGCAG 1 cut(s) 845
BspMI ACCTGC 1 cut(s) 594
BspPI GGATC 4 cut(s) 415, 498, 1381, 1476
BspQI GCTCTTC 1 cut(s) 706
BspT107I GGYRCC 1 cut(s) 654
BspTNI GGTCTC 1 cut(s) 1073
BsrBI CCGCTC 1 cut(s) 676
BssECI CCNNGG 3 cut(s) 452, 1236, 1369
BssMI GATC 6 cut(s) 346, 407, 490, 1373, 1396, 1468
BssNI GRCGYC 1 cut(s) 655
BssT1I CCWWGG 2 cut(s) 1236, 1369
Bst2UI CCWGG 3 cut(s) 470, 708, 1134
Bst4CI ACNGT 2 cut(s) 957, 1443
Bst6I CTCTTC 4 cut(s) 271, 706, 1179, 1204
BstACI GRCGYC 1 cut(s) 655
BstAPI GCANNNNNTGC 1 cut(s) 1483
BstBAI YACGTR 1 cut(s) 850
BstC8I GCNNGC 6 cut(s) 459, 845, 1000, 1168, 1252, 1488
BstDEI CTNAG 9 cut(s) 423, 494, 690, 729, 816, 1049, 1301, 1354, 1520
BstENI CCTNNNNNAGG 3 cut(s) 268, 693, 1269
BstF5I GGATG 2 cut(s) 703, 897
BstFNI CGCG 1 cut(s) 435
BstH2I RGCGCY 1 cut(s) 658
BstHHI GCGC 3 cut(s) 435, 657, 1233
BstKTI GATC 6 cut(s) 349, 410, 493, 1376, 1399, 1471
BstMAI GTCTC 1 cut(s) 1073
BstMBI GATC 6 cut(s) 346, 407, 490, 1373, 1396, 1468
BstMWI GCNNNNNNNGC 5 cut(s) 424, 685, 1167, 1483, 1492
BstNI CCWGG 3 cut(s) 470, 708, 1134
BstSCI CCNGG 3 cut(s) 468, 706, 1132
BstSFI CTRYAG 1 cut(s) 841
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 3 cut(s) 827, 1462, 1502
BstV2I GAAGAC 4 cut(s) 261, 390, 554, 922
BstX2I RGATCY 1 cut(s) 490
BstXI CCANNNNNNTGG 1 cut(s) 476
BstYI RGATCY 1 cut(s) 490
BsuI GTATCC 2 cut(s) 459, 969
BsuRI GGCC 3 cut(s) 457, 880, 1047
BtgZI GCGATG 1 cut(s) 1431
BtsCI GGATG 2 cut(s) 703, 897
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 1 cut(s) 219
BveI ACCTGC 1 cut(s) 594
Cac8I GCNNGC 6 cut(s) 459, 845, 1000, 1168, 1252, 1488
CciI TCATGA 2 cut(s) 970, 1075
CfoI GCGC 3 cut(s) 435, 657, 1233
Csp6I GTAC 1 cut(s) 126
CviAII CATG 5 cut(s) 391, 824, 971, 1076, 1483
CviQI GTAC 1 cut(s) 126
DdeI CTNAG 9 cut(s) 423, 494, 690, 729, 816, 1049, 1301, 1354, 1520
DinI GGCGCC 1 cut(s) 656
DpnI GATC 6 cut(s) 348, 409, 492, 1375, 1398, 1470
DpnII GATC 6 cut(s) 346, 407, 490, 1373, 1396, 1468
Eam1104I CTCTTC 4 cut(s) 271, 706, 1179, 1204
EarI CTCTTC 4 cut(s) 271, 706, 1179, 1204
EciI GGCGGA 1 cut(s) 694
Ecl136II GAGCTC 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 1236, 1369
Eco147I AGGCCT 2 cut(s) 880, 1047
Eco24I GRGCYC 1 cut(s) 284
Eco31I GGTCTC 1 cut(s) 1073
Eco53kI GAGCTC 1 cut(s) 282
Eco57I CTGAAG 3 cut(s) 45, 1107, 1254
EcoICRI GAGCTC 1 cut(s) 282
EcoNI CCTNNNNNAGG 3 cut(s) 268, 693, 1269
EcoRI GAATTC 2 cut(s) 1218, 1279
EcoRII CCWGG 3 cut(s) 468, 706, 1132
EcoT14I CCWWGG 2 cut(s) 1236, 1369
EcoT38I GRGCYC 1 cut(s) 284
EgeI GGCGCC 1 cut(s) 656
EheI GGCGCC 1 cut(s) 656
ErhI CCWWGG 2 cut(s) 1236, 1369
FaeI CATG 5 cut(s) 394, 827, 974, 1079, 1486
FaqI GGGAC 2 cut(s) 880, 1290
FatI CATG 5 cut(s) 390, 823, 970, 1075, 1482
FauI CCCGC 1 cut(s) 857
FbaI TGATCA 1 cut(s) 346
FblI GTMKAC 2 cut(s) 230, 1402
Fnu4HI GCNGC 4 cut(s) 677, 841, 1451, 1516
FokI GGATG 2 cut(s) 690, 904
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 4 cut(s) 677, 841, 1451, 1516
FspBI CTAG 3 cut(s) 81, 332, 1290
GlaI GCGC 3 cut(s) 434, 656, 1232
GluI GCNGC 4 cut(s) 677, 841, 1451, 1516
GsaI CCCAGC 1 cut(s) 720
GsuI CTGGAG 1 cut(s) 280
HaeII RGCGCY 1 cut(s) 658
HaeIII GGCC 3 cut(s) 457, 880, 1047
HhaI GCGC 3 cut(s) 435, 657, 1233
Hin1I GRCGYC 1 cut(s) 655
Hin1II CATG 5 cut(s) 394, 827, 974, 1079, 1486
Hin6I GCGC 3 cut(s) 433, 655, 1231
HinP1I GCGC 3 cut(s) 433, 655, 1231
HincII GTYRAC 2 cut(s) 568, 593
HindII GTYRAC 2 cut(s) 568, 593
HphI GGTGA 4 cut(s) 263, 632, 845, 1289
Hpy166II GTNNAC 7 cut(s) 231, 292, 541, 568, 593, 1061, 1403
Hpy188I TCNGA 6 cut(s) 25, 49, 693, 1014, 1357, 1523
Hpy8I GTNNAC 7 cut(s) 231, 292, 541, 568, 593, 1061, 1403
HpyAV CCTTC 5 cut(s) 20, 749, 854, 945, 1275
HpyCH4III ACNGT 2 cut(s) 957, 1443
HpyCH4IV ACGT 1 cut(s) 849
HpyCH4V TGCA 9 cut(s) 190, 212, 483, 662, 776, 843, 916, 998, 1486
HpyF10VI GCNNNNNNNGC 5 cut(s) 424, 685, 1167, 1483, 1492
HpyF3I CTNAG 9 cut(s) 423, 494, 690, 729, 816, 1049, 1301, 1354, 1520
HpySE526I ACGT 1 cut(s) 849
Hsp92I GRCGYC 1 cut(s) 655
Hsp92II CATG 5 cut(s) 394, 827, 974, 1079, 1486
HspAI GCGC 3 cut(s) 433, 655, 1231
KasI GGCGCC 1 cut(s) 654
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 6 cut(s) 346, 407, 490, 1373, 1396, 1468
LguI GCTCTTC 1 cut(s) 706
LmnI GCTCC 5 cut(s) 12, 287, 503, 1418, 1458
Lsp1109I GCAGC 3 cut(s) 827, 1462, 1502
LweI GCATC 5 cut(s) 199, 785, 882, 919, 1495
MaeI CTAG 3 cut(s) 81, 332, 1290
MaeII ACGT 1 cut(s) 849
MaeIII GTNAC 3 cut(s) 833, 898, 1295
MalI GATC 6 cut(s) 348, 409, 492, 1375, 1398, 1470
MbiI CCGCTC 1 cut(s) 676
MboI GATC 6 cut(s) 346, 407, 490, 1373, 1396, 1468
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 3 cut(s) 221, 284, 1168
Mly113I GGCGCC 1 cut(s) 655
MlyI GAGTC 5 cut(s) 287, 709, 862, 1303, 1312
MmeI TCCRAC 1 cut(s) 1480
MroXI GAANNNNTTC 2 cut(s) 58, 1279
MseI TTAA 2 cut(s) 326, 1531
MslI CAYNNNNRTG 2 cut(s) 224, 354
MspR9I CCNGG 3 cut(s) 470, 708, 1134
Mva1269I GAATGC 2 cut(s) 435, 916
MvaI CCWGG 3 cut(s) 470, 708, 1134
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 5 cut(s) 424, 685, 1167, 1483, 1492
NarI GGCGCC 1 cut(s) 655
NdeII GATC 6 cut(s) 346, 407, 490, 1373, 1396, 1468
NlaIII CATG 5 cut(s) 394, 827, 974, 1079, 1486
NlaIV GGNNCC 3 cut(s) 656, 725, 1420
NmeAIII GCCGAG 1 cut(s) 433
NmuCI GTSAC 2 cut(s) 833, 1295
PagI TCATGA 2 cut(s) 970, 1075
PceI AGGCCT 2 cut(s) 880, 1047
PciSI GCTCTTC 1 cut(s) 706
PcsI WCGNNNNNNNCGW 3 cut(s) 255, 275, 551
PctI GAATGC 2 cut(s) 435, 916
PdmI GAANNNNTTC 2 cut(s) 58, 1279
PfeI GAWTC 8 cut(s) 5, 71, 197, 260, 308, 1146, 1350, 1524
PfoI TCCNGGA 2 cut(s) 706, 1132
PkrI GCNGC 4 cut(s) 678, 842, 1452, 1517
PleI GAGTC 5 cut(s) 287, 708, 862, 1302, 1311
PluTI GGCGCC 1 cut(s) 658
PpsI GAGTC 5 cut(s) 287, 708, 862, 1302, 1311
Ppu21I YACGTR 1 cut(s) 850
Psp124BI GAGCTC 1 cut(s) 284
Psp6I CCWGG 3 cut(s) 468, 706, 1132
PspFI CCCAGC 1 cut(s) 716
PspGI CCWGG 3 cut(s) 468, 706, 1132
PspN4I GGNNCC 3 cut(s) 656, 725, 1420
PstI CTGCAG 1 cut(s) 845
PsuI RGATCY 1 cut(s) 490
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
RseI CAYNNNNRTG 2 cut(s) 224, 354
SacI GAGCTC 1 cut(s) 284
SapI GCTCTTC 1 cut(s) 706
SaqAI TTAA 2 cut(s) 326, 1531
SatI GCNGC 4 cut(s) 677, 841, 1451, 1516
Sau3AI GATC 6 cut(s) 346, 407, 490, 1373, 1396, 1468
SchI GAGTC 5 cut(s) 287, 709, 862, 1303, 1312
ScrFI CCNGG 3 cut(s) 470, 708, 1134
SduI GDGCHC 3 cut(s) 221, 284, 1168
SfaNI GCATC 5 cut(s) 199, 785, 882, 919, 1495
SfcI CTRYAG 1 cut(s) 841
SfoI GGCGCC 1 cut(s) 656
SmiMI CAYNNNNRTG 2 cut(s) 224, 354
SseBI AGGCCT 2 cut(s) 880, 1047
SsiI CCGC 4 cut(s) 676, 679, 864, 1083
SspDI GGCGCC 1 cut(s) 654
SspMI CTAG 3 cut(s) 81, 332, 1290
SstI GAGCTC 1 cut(s) 284
StuI AGGCCT 2 cut(s) 880, 1047
StyD4I CCNGG 3 cut(s) 468, 706, 1132
StyI CCWWGG 2 cut(s) 1236, 1369
TaaI ACNGT 2 cut(s) 957, 1443
TaiI ACGT 1 cut(s) 852
TaqI TCGA 4 cut(s) 269, 278, 545, 1428
TaqII GACCGA 1 cut(s) 998
TatI WGTACW 1 cut(s) 125
TauI GCSGC 1 cut(s) 679
TfiI GAWTC 8 cut(s) 5, 71, 197, 260, 308, 1146, 1350, 1524
Tru1I TTAA 2 cut(s) 326, 1531
Tru9I TTAA 2 cut(s) 326, 1531
TscAI CASTG 1 cut(s) 219
TseFI GTSAC 2 cut(s) 833, 1295
TseI GCWGC 3 cut(s) 840, 1450, 1515
Tsp45I GTSAC 2 cut(s) 833, 1295
TspDTI ATGAA 7 cut(s) 350, 390, 599, 959, 987, 1064, 1292
TspGWI ACGGA 1 cut(s) 993
TspRI CASTG 1 cut(s) 219
XagI CCTNNNNNAGG 3 cut(s) 268, 693, 1269
XapI RAATTY 6 cut(s) 238, 623, 854, 1218, 1259, 1279
XbaI TCTAGA 1 cut(s) 80
XmiI GTMKAC 2 cut(s) 230, 1402
XmnI GAANNNNTTC 2 cut(s) 58, 1279
XspI CTAG 3 cut(s) 81, 332, 1290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.