FvH4_5g13670

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009576
Physical Location & Seq
Forward (+)
7728793 .. 7735148
6356 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13670.t1

Sequence Viewer

Length: 4380 bp
ATGTATGGCCAACCTAATTATGGTCCGCCGTTTGCGCAGGGTCAGACACCAATGCCACCTGCAAACCAGCAGCGTCCGCCGCCCCTGCCACCACCACCGCCTCCCTTTACAGCACCACACAATGTAGTTACATCTGCAGCCCCACCGCAGCCAGGTCCTTATTTATACCAGCACGGTTTTGTTCCTGCTGCTCACCAAAGCCCGCCTGTTTTGTTCAGTGGGATGATGACTACAGGTCCATCGTATGGTGGAAACACATGGCATAACGCGCAGCGTCCTCCAACTACAGGAAACCAGAATTTCCAACGGACTCATCCACCGGCACTTGTGACTCCTCATGCTGCTCCTTACCCAAACATGTCACAGGCTGCAGTACCACATGGAGCATTACCGCCTCCTCCCCCTCAAGCGGCATCTGCTCAGTCACCTGCTCCACCGTTGCCCACCGCTCCTTCACCTTCAACTTCATTGCAAAACCTTGATCCTGATCATGGGTCTAATAAACTTCGCTTGGGTGAAATGAGGGGCATGAATAACCAAGTCACAGCCAGTAACAATTTCGAACATTTCACATCTGTGCATGAGCACTCTCAAAATGTTCAAGGTGGAAGTGAATGTGAAAAAGCAGGTTCTCTTGCTAGAGATGGAGTATCATCAAATGGAAGTGTGATGTTAAACATTTCTTCACCTCCACCTAAGCCATCAGATGACATAGTTCATAAGATTGATTCTATCTGCCAACTTAGTGCTATGAATGAAGGGAAAATTGAATCACCATCAAAGTCTTCGCAGATTGACTCTTCATCTCAACCTGACCAGTTTATGGTTTCTTCAGGTTATAGCTTGCCTGCTGATTCTGACATGGAGATGGAAGATGATATTACCTTGTCTGACGGAACTCAGGAAGTTCATAATTCATCTGACGCTCTGAACCGCACGTCTGATATCAATCATGGGGAGCTTGATGTCAAAAAGCACCTACATGGAGCACAGAGTTCACCAGAATGGACTGATCCCCAGGGTGTCTCATTTGAAAAGGTGCCGTCCTCTTTGTCCGAATGCCAATTAATAATTCAAGGGGCACCATCCAGAGTTGATATTTGTTCAACTGGAGCTTCTGAATCCCCTCTGGCAAGTCAACAAGAGAAATCTTCGATTCCCAAAGCTGATGATCAGAATAAACTTGATGCTTCTGCAGCGGCTGAGGCAACAACTTCAGATGGGTTTTCTAACCATATTACAGTTAACAGTCCATTTAGACTTCTACAAGACTATGCCTCTGAAAACAGTTCAGAAGATGGTGATGTTGGTATACCTCCATCATCAGTTACAACTAACGTTAAAAGTAGTGCTAAAGATGCTGGGTCTCAGTTTGAGATAGGATCTAAGAACCCATGCATGACTGATAAAATGTCTGGGCGGCAATATGAATCCAGAAGGTCTAAATTCTCCTTGGATACAAAAAAAGATGTTAGGAGTACTGATACAACATTAATCATTGAGAGTCATGAGGCTTTCCAGGGAAAAGATGCATTGAATGGATCTCCTATTGATATCGCTTTCAAAAGGGACAAGTCTCAAGAAGGGAAAAAGACCAAGTCTGAATGTCTGCCAAAGGTGGATGAGTATGGAAGATTGGTCAGAGAAGGTTCCAGTGACAGTAACTCTGATGGTTCACATTATAATAAGAGGCGTAAAAGAGGCAGAAGCCGCAGTCGTAGTCGTAGTCGCAGCCGTAAACGGAGCCGCAGCCGCAGTCGGTCTCCTCTTGACAGTAGGAGGAGGAGGAGTCCTCCGAGGAGGAGAGAGAAGCGAAACCGTTCGCCCAGTTGGTCTTCTAGGAATCAAAGAGGCAGGAGCAGGTCTCCCACATTTAGGCGTGCAGGTGAGTTCAGAGATGAAAATAAGAGACAGGACAGACGCCACATTCCAGAATGCTTCGACTTCCTTCGAGGCAAATGCTCCCGTGGAGGATCCTGTCGGTATATGCACAGTGAGCATGACCGAAATGATGGTTCATGGCGCCATAGGAACCAGCAAAAGCATCTAGAAGTTCAGTCTAGTGTGAAGAAATCTAGGATTAATGAAGAGATTGAAGATAGCTCTGACATGAGACTGCATGGTGAAGCCAAAGGTCAGGAGATGCAAATCTATCCTGATATGATCACAAAAGATGGTCAGTTTAATGATACTGATAAAACTGATTACAAGAGTTCTAAAATGACAGCTGCTACAGTGCAAGTGAAACAAACTCTTCTTGGGAAGTCGGAGGAACACAGCGCTCACAATCCTGAATCTCACCACCCATCCGCTGAAATGTTGAGCAGTGTTGATAATATGAAGTCACGGCGTGATACTTCTCAGGCAATTGAACAATCTCGAAGCATTAATTTTGCGGGAGAGGTTCAAAAGGCTTATTATCCATCTCAGCAGATGGAAGCTTCTCTTGTATCTGATTCACCACCTGATCGACCATCCAAGACTTCTCCATATAAGGTTTCTAGTATTGAGCCGGCAGCAGATGCAATCTTATCAATTCAATCATGTCCCACCGAAAGTTCTAATGCTCAACCACTTTCATCAGGACAGTTTTCATCACAGTTTTTGGCTCCTAAAGAGTCACCTCTGCCCGGTTTTTCTGCTGCAAATAGTCCATATCCTTCAAAGTTGCCTCCTCCTCCTCCTCCTTCACTATCACAAGGTACCAGTGTTGCTCATGTGCCACAGTTGCATAGGGACTACAGCCAGAGGCCACCCTATCCAGTTCAGTCCATTCCTACAGGAACTATGCATGCACATGCTTATCAAGGTCCCTTATCCAACCAACCTTCCCAATTCCCTGTATCACAAGAATCAACTTGGCCATCCTTGCCACCGCCACCTCCACGACCACCATATGATTCATCTTTAAATCCAGGAACTGCTGCACAAGATGCTTCTTCACACTTTCAGCAGAATCACTTGGCTCCAAGGAGTGACTTCGGTTCTCAGAGTTCTATGAGGCCTTACGGCTCTTACCCAACTGAATCCCCTCATTCTAAGGGTGAGTTTCTGCATCAGATGTATCCTACGTTGTCAGAGCTTCCTCATCCTCCACCAAACAGGGAAGATTTCGGATCAGGCAATCCGTCAAATCAACATTTTGGGGGCCCTGATCATATGAGAGAAGATCGCTTTACACATGCTCCTGTGCAGAATGTAAATCCTTCACACTCTTTTGCTCAGGGACACACACACCCTCAACCCCCACCTCCTTCACAGGAATTAACGAGGATCAAGATGAAAAATTTCTCTGGCGACAATTTCCCAGTCGGGGAACTTTTCAATTCATCATCACAAATTCATCCTCATTCACATAACCAACAGCCTAGCTATGGTGTTGGTGATAGCATATTGGGTGTGCCTGGGAAGACTGGTGCACAATACCCTGTAGGTGGTAGCATTTTGGGTTTCCCTGGGAAAGATGGGCCCATGTCCCGATACCCTCCAGATATGCCTGACAGAAGTCAGTTTTCTCAAGTTCCTGATTTTGGGGAATCTAGAATTCAAACACATCATAATCCTTACGCAGCTACTTTTGAGCAACCATTGAGCTTCAAGTTTAGTTCTAACACTCTCATTCAAGGAAAGAATGCGCCTGCTGCTAACATGTTTGATACACCGGTCCAAGTCCCAGTCGATGGGCAAGGAGTGGGCAGTGCTGGATCAAGACAGACAACCTCCTCGCCAAGTTCTGCTGGAGCTGTTAGTCAGCTTTTGCGCAAGTCAGTTAGTGAGCAGTATGATCCACTTTTGGACAGCATTGAGCCATCGGGAAATCCGCTCAATAAACATGATCAGAGCCAAAAGCATACAGCCACCAATGATTCAAATATGATGGTGAGGTTCAGTGGTTCTTGTGAGCCATTAGATGTGGAAGAGAACAAGAAACATACAGAGGTTGGCCCTGTTGCATCGGCTACATCTCTGGATAATGACGGCTATGGTGAGACAGCAGATGCAGAAGTGGGTGTCGTCGAGGATGAGAGTCTTAGTAATGATGATGGTGGAGCAAACATGGCTGTAGGAGAGATGGAGATTGATCAGGTGAAATCTGGAGGGAAGAGCAGGAAGAAAAAGGATTCCAGGTCAACTAGACTTTTCAAGAGTGCTGTTGCAGATTTTGTAAAGGACCTTCTGAAGCCATCATGGCGGCAGGGTAATATGAGCAAAGAAGCCTTTAAGACCATTGTTAAAAAGACTGTTGATAAGGTATCTGGAGCCATGAAGAGTCACCAGATACCGAAGTCCGAGGCAAAGATAAATCACTACATTGACTCATCTCAGCGGAAACTAACAAAGCTTGTGATGGGTTATGTTGATAAGTATGTCAAGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Surp

Protein Analysis

1460

Amino Acids

159.24

Weight (kDa)

7.73

Isoelectric Point (pI)

70.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAF11-like_C PF23030 1378 - 1443 3.6e-06 SCAF11-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1683
AarI CACCTGC 3 cut(s) 67, 436, 1874
Acc16I TGCGCA 2 cut(s) 36, 3761
Acc36I ACCTGC 5 cut(s) 67, 436, 617, 1851, 1874
Acc65I GGTACC 1 cut(s) 2702
AccB1I GGYRCC 4 cut(s) 1039, 1081, 2022, 2702
AccB7I CCANNNNNTGG 3 cut(s) 245, 823, 3680
AccBSI CCGCTC 2 cut(s) 449, 3823
AccI GTMKAC 1 cut(s) 1312
AccII CGCG 1 cut(s) 269
AclI AACGTT 1 cut(s) 1338
AcoI YGGCCR 2 cut(s) 7, 2861
AcsI RAATTY 5 cut(s) 298, 1445, 3286, 3339, 3543
AcuI CTGAAG 3 cut(s) 816, 1200, 4199
AcyI GRCGYC 2 cut(s) 1921, 2023
AdeI CACNNNGTG 1 cut(s) 2351
AfaI GTAC 3 cut(s) 375, 1480, 2704
AfeI AGCGCT 1 cut(s) 2281
AflIII ACRYGT 2 cut(s) 357, 3648
AgeI ACCGGT 1 cut(s) 3661
AhdI GACNNNNNGTC 1 cut(s) 234
AjiI CACGTC 1 cut(s) 939
AjnI CCWGG 7 cut(s) 151, 1017, 1518, 2914, 3403, 3454, 4124
AjuI GAANNNNNNNTTGG 2 cut(s) 1056, 1088
AleI CACNNNNGTG 1 cut(s) 575
Alw21I GWGCWC 3 cut(s) 588, 991, 3421
Alw26I GTCTC 8 cut(s) 1030, 1371, 1581, 1767, 1869, 1903, 2107, 3983
Alw44I GTGCAC 1 cut(s) 3417
AlwNI CAGNNNCTG 2 cut(s) 1202, 2921
Aor51HI AGCGCT 1 cut(s) 2281
AoxI GGCC 7 cut(s) 7, 2750, 2861, 3002, 3148, 3467, 3943
ApaI GGGCCC 2 cut(s) 3152, 3471
ApaLI GTGCAC 1 cut(s) 3417
ApoI RAATTY 5 cut(s) 298, 1445, 3286, 3339, 3543
AseI ATTAAT 4 cut(s) 1067, 1493, 2082, 2388
AsiGI ACCGGT 1 cut(s) 3661
Asp700I GAANNNNTTC 2 cut(s) 1819, 3287
Asp718I GGTACC 1 cut(s) 2702
AspLEI GCGC 6 cut(s) 37, 271, 2025, 2282, 3637, 3762
AsuC2I CCSGG 1 cut(s) 2631
AsuII TTCGAA 1 cut(s) 561
AvaII GGWCC 6 cut(s) 23, 155, 236, 2810, 3664, 4171
BaeGI GKGCMC 4 cut(s) 1084, 3152, 3421, 3471
BaeI ACNNNNGTAYC 6 cut(s) 2694, 2727, 3648, 3681, 4271, 4304
BalI TGGCCA 2 cut(s) 9, 2863
BamHI GGATCC 1 cut(s) 1973
BanI GGYRCC 4 cut(s) 1039, 1081, 2022, 2702
BanII GRGCYC 2 cut(s) 3152, 3471
BarI GAAGNNNNNNTAC 2 cut(s) 4158, 4190
BbsI GAAGAC 3 cut(s) 777, 1827, 3416
Bbv12I GWGCWC 3 cut(s) 588, 991, 3421
BbvCI CCTCAGC 1 cut(s) 1203
BceAI ACGGC 6 cut(s) 13, 1027, 1719, 2363, 3025, 3994
BcgI CGANNNNNNTGC 2 cut(s) 1941, 1975
BciT130I CCWGG 7 cut(s) 153, 1019, 1520, 2916, 3405, 3456, 4126
BciVI GTATCC 2 cut(s) 1450, 3075
BclI TGATCA 6 cut(s) 487, 1171, 2163, 3154, 3835, 4081
BcnI CCSGG 1 cut(s) 2631
BcoDI GTCTC 8 cut(s) 1030, 1371, 1581, 1767, 1869, 1903, 2107, 3983
BfaI CTAG 9 cut(s) 639, 1839, 2048, 2061, 2076, 2502, 3369, 3540, 4134
BfoI RGCGCY 2 cut(s) 2026, 2283
BfuAI ACCTGC 5 cut(s) 67, 436, 617, 1851, 1874
BfuI GTATCC 2 cut(s) 1450, 3075
BglI GCCNNNNNGGC 1 cut(s) 4189
BmcAI AGTACT 1 cut(s) 1480
Bme1390I CCNGG 8 cut(s) 153, 1019, 1520, 2631, 2916, 3405, 3456, 4126
Bme18I GGWCC 6 cut(s) 23, 155, 236, 2810, 3664, 4171
BmeRI GACNNNNNGTC 1 cut(s) 234
BmgBI CACGTC 1 cut(s) 939
BmrFI CCNGG 8 cut(s) 153, 1019, 1520, 2631, 2916, 3405, 3456, 4126
BmrI ACTGGG 3 cut(s) 1821, 3302, 3668
BmuI ACTGGG 3 cut(s) 1821, 3302, 3668
BoxI GACNNNNGTC 1 cut(s) 3504
BpiI GAAGAC 3 cut(s) 777, 1827, 3416
BplI GAGNNNNNCTC 4 cut(s) 1777, 1809, 1849, 1881
BpmI CTGGAG 5 cut(s) 1131, 3471, 3759, 4116, 4278
Bpu10I CCTNAGC 3 cut(s) 696, 1203, 3222
Bpu14I TTCGAA 1 cut(s) 561
BpuEI CTTGAG 3 cut(s) 390, 1563, 3501
BpuMI CCSGG 1 cut(s) 2631
BsaBI GATNNNNATC 3 cut(s) 486, 948, 2147
BsaHI GRCGYC 2 cut(s) 1921, 2023
BsaI GGTCTC 3 cut(s) 1371, 1767, 1869
BsaWI WCCGGW 1 cut(s) 3661
BsaXI ACNNNNNCTCC 4 cut(s) 1774, 1804, 3169, 3199
Bse118I RCCGGY 3 cut(s) 319, 2512, 3661
Bse3DI GCAATG 1 cut(s) 467
Bse8I GATNNNNATC 3 cut(s) 486, 948, 2147
BseBI CCWGG 7 cut(s) 153, 1019, 1520, 2916, 3405, 3456, 4126
BseJI GATNNNNATC 3 cut(s) 486, 948, 2147
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 9 cut(s) 434, 914, 1194, 1382, 2375, 2441, 3002, 3236, 4337
BseSI GKGCMC 4 cut(s) 1084, 3152, 3421, 3471
BseYI CCCAGC 1 cut(s) 1361
BsgI GTGCAG 3 cut(s) 1902, 2910, 3212
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 7 cut(s) 9, 2752, 2863, 3004, 3150, 3469, 3945
BshNI GGYRCC 4 cut(s) 1039, 1081, 2022, 2702
BshTI ACCGGT 1 cut(s) 3661
BsiHKAI GWGCWC 3 cut(s) 588, 991, 3421
BsiSI CCGG 4 cut(s) 320, 2513, 2631, 3662
BslFI GGGAC 7 cut(s) 1583, 2532, 2750, 2796, 3240, 3460, 3656
BsmAI GTCTC 8 cut(s) 1030, 1371, 1581, 1767, 1869, 1903, 2107, 3983
BsmFI GGGAC 7 cut(s) 1583, 2532, 2750, 2796, 3240, 3460, 3656
BsmI GAATGC 3 cut(s) 1064, 1940, 3637
BsnI GGCC 7 cut(s) 9, 2752, 2863, 3004, 3150, 3469, 3945
Bso31I GGTCTC 3 cut(s) 1371, 1767, 1869
Bsp119I TTCGAA 1 cut(s) 561
Bsp120I GGGCCC 2 cut(s) 3148, 3467
Bsp1286I GDGCHC 6 cut(s) 588, 991, 1084, 3152, 3421, 3471
BspANI GGCC 7 cut(s) 9, 2752, 2863, 3004, 3150, 3469, 3945
BspCNI CTCAG 9 cut(s) 433, 913, 1195, 1381, 2374, 2440, 3001, 3235, 4336
BspFNI CGCG 1 cut(s) 269
BspHI TCATGA 1 cut(s) 1507
BspMAI CTGCAG 3 cut(s) 139, 373, 1198
BspMI ACCTGC 5 cut(s) 67, 436, 617, 1851, 1874
BspQI GCTCTTC 1 cut(s) 4097
BspT104I TTCGAA 1 cut(s) 561
BspT107I GGYRCC 4 cut(s) 1039, 1081, 2022, 2702
BspTNI GGTCTC 3 cut(s) 1371, 1767, 1869
BsrBI CCGCTC 2 cut(s) 449, 3823
BsrDI GCAATG 1 cut(s) 467
BsrFI RCCGGY 3 cut(s) 319, 2512, 3661
BssAI RCCGGY 3 cut(s) 319, 2512, 3661
BssNAI GTATAC 1 cut(s) 1313
BssNI GRCGYC 2 cut(s) 1921, 2023
BssT1I CCWWGG 2 cut(s) 1452, 2969
Bst1107I GTATAC 1 cut(s) 1313
Bst2UI CCWGG 7 cut(s) 153, 1019, 1520, 2916, 3405, 3456, 4126
Bst6I CTCTTC 6 cut(s) 805, 2082, 2259, 3912, 4097, 4262
BstACI GRCGYC 2 cut(s) 1921, 2023
BstAPI GCANNNNNTGC 2 cut(s) 2522, 2933
BstBI TTCGAA 1 cut(s) 561
BstC8I GCNNGC 7 cut(s) 203, 845, 849, 1881, 2514, 2793, 3639
BstDSI CCRYGG 1 cut(s) 1966
BstFNI CGCG 1 cut(s) 269
BstH2I RGCGCY 2 cut(s) 2026, 2283
BstHHI GCGC 6 cut(s) 37, 271, 2025, 2282, 3637, 3762
BstMAI GTCTC 8 cut(s) 1030, 1371, 1581, 1767, 1869, 1903, 2107, 3983
BstNI CCWGG 7 cut(s) 153, 1019, 1520, 2916, 3405, 3456, 4126
BstNSI RCATGY 5 cut(s) 361, 2795, 2801, 3185, 3652
BstPAI GACNNNNGTC 1 cut(s) 3504
BstSCI CCNGG 8 cut(s) 151, 1017, 1518, 2629, 2914, 3403, 3454, 4124
BstSLI GKGCMC 4 cut(s) 1084, 3152, 3421, 3471
BstUI CGCG 1 cut(s) 269
BstV2I GAAGAC 3 cut(s) 777, 1827, 3416
BstX2I RGATCY 3 cut(s) 1382, 1541, 1973
BstYI RGATCY 3 cut(s) 1382, 1541, 1973
BstZ17I GTATAC 1 cut(s) 1313
BsuI GTATCC 2 cut(s) 1450, 3075
BsuRI GGCC 7 cut(s) 9, 2752, 2863, 3004, 3150, 3469, 3945
BtgI CCRYGG 1 cut(s) 1966
BtrI CACGTC 1 cut(s) 939
BtsI GCAGTG 2 cut(s) 2332, 3703
BtsIMutI CAGTG 8 cut(s) 223, 1660, 1999, 2241, 2332, 2713, 3703, 3895
BveI ACCTGC 5 cut(s) 67, 436, 617, 1851, 1874
Cac8I GCNNGC 7 cut(s) 203, 845, 849, 1881, 2514, 2793, 3639
CaiI CAGNNNCTG 2 cut(s) 1202, 2921
CciI TCATGA 1 cut(s) 1507
CfoI GCGC 6 cut(s) 37, 271, 2025, 2282, 3637, 3762
Cfr10I RCCGGY 3 cut(s) 319, 2512, 3661
CseI GACGC 4 cut(s) 62, 263, 932, 1929
Csp6I GTAC 3 cut(s) 374, 1479, 2703
CspAI ACCGGT 1 cut(s) 3661
CviQI GTAC 3 cut(s) 374, 1479, 2703
DinI GGCGCC 1 cut(s) 2024
DraI TTTAAA 1 cut(s) 2910
DraIII CACNNNGTG 1 cut(s) 2351
DriI GACNNNNNGTC 1 cut(s) 234
EaeI YGGCCR 2 cut(s) 7, 2861
Eam1104I CTCTTC 6 cut(s) 805, 2082, 2259, 3912, 4097, 4262
Eam1105I GACNNNNNGTC 1 cut(s) 234
EarI CTCTTC 6 cut(s) 805, 2082, 2259, 3912, 4097, 4262
EciI GGCGGA 2 cut(s) 15, 66
Eco130I CCWWGG 2 cut(s) 1452, 2969
Eco147I AGGCCT 1 cut(s) 3004
Eco24I GRGCYC 2 cut(s) 3152, 3471
Eco31I GGTCTC 3 cut(s) 1371, 1767, 1869
Eco32I GATATC 2 cut(s) 946, 1555
Eco47I GGWCC 6 cut(s) 23, 155, 236, 2810, 3664, 4171
Eco47III AGCGCT 1 cut(s) 2281
Eco57I CTGAAG 3 cut(s) 816, 1200, 4199
EcoO109I RGGNCCY 5 cut(s) 155, 2810, 3148, 3149, 4171
EcoRI GAATTC 1 cut(s) 3543
EcoRII CCWGG 7 cut(s) 151, 1017, 1518, 2914, 3403, 3454, 4124
EcoRV GATATC 2 cut(s) 946, 1555
EcoT14I CCWWGG 2 cut(s) 1452, 2969
EcoT22I ATGCAT 3 cut(s) 1400, 1534, 2793
EcoT38I GRGCYC 2 cut(s) 3152, 3471
EgeI GGCGCC 1 cut(s) 2024
EheI GGCGCC 1 cut(s) 2024
ErhI CCWWGG 2 cut(s) 1452, 2969
FalI AAGNNNNNCTT 2 cut(s) 2430, 2462
FaqI GGGAC 7 cut(s) 1583, 2532, 2750, 2796, 3240, 3460, 3656
FauI CCCGC 2 cut(s) 210, 2389
FauNDI CATATG 2 cut(s) 2896, 3159
FbaI TGATCA 6 cut(s) 487, 1171, 2163, 3154, 3835, 4081
FblI GTMKAC 1 cut(s) 1312
FriOI GRGCYC 2 cut(s) 3152, 3471
FspBI CTAG 9 cut(s) 639, 1839, 2048, 2061, 2076, 2502, 3369, 3540, 4134
FspI TGCGCA 2 cut(s) 36, 3761
GlaI GCGC 6 cut(s) 36, 270, 2024, 2281, 3636, 3761
GsaI CCCAGC 1 cut(s) 1365
GsuI CTGGAG 5 cut(s) 1131, 3471, 3759, 4116, 4278
HaeII RGCGCY 2 cut(s) 2026, 2283
HaeIII GGCC 7 cut(s) 9, 2752, 2863, 3004, 3150, 3469, 3945
HapII CCGG 4 cut(s) 320, 2513, 2631, 3662
HgaI GACGC 4 cut(s) 62, 263, 932, 1929
HhaI GCGC 6 cut(s) 37, 271, 2025, 2282, 3637, 3762
Hin1I GRCGYC 2 cut(s) 1921, 2023
Hin6I GCGC 6 cut(s) 35, 269, 2023, 2280, 3635, 3760
HinP1I GCGC 6 cut(s) 35, 269, 2023, 2280, 3635, 3760
HincII GTYRAC 3 cut(s) 1139, 1246, 4131
HindII GTYRAC 3 cut(s) 1139, 1246, 4131
HindIII AAGCTT 2 cut(s) 2439, 4340
HpaI GTTAAC 1 cut(s) 1246
HpaII CCGG 4 cut(s) 320, 2513, 2631, 3662
Hpy166II GTNNAC 8 cut(s) 998, 1139, 1246, 1313, 1676, 1739, 3419, 4131
Hpy8I GTNNAC 8 cut(s) 998, 1139, 1246, 1313, 1676, 1739, 3419, 4131
Hpy99I CGWCG 1 cut(s) 4019
HpyCH4IV ACGT 3 cut(s) 938, 1338, 3071
HpySE526I ACGT 3 cut(s) 938, 1338, 3071
Hsp92I GRCGYC 2 cut(s) 1921, 2023
HspAI GCGC 6 cut(s) 35, 269, 2023, 2280, 3635, 3760
KasI GGCGCC 1 cut(s) 2022
KpnI GGTACC 1 cut(s) 2706
KroI GCCGGC 1 cut(s) 2512
KroNI GCCGGC 1 cut(s) 2514
Ksp22I TGATCA 6 cut(s) 487, 1171, 2163, 3154, 3835, 4081
KspAI GTTAAC 1 cut(s) 1246
LguI GCTCTTC 1 cut(s) 4097
MaeI CTAG 9 cut(s) 639, 1839, 2048, 2061, 2076, 2502, 3369, 3540, 4134
MaeII ACGT 3 cut(s) 938, 1338, 3071
MbiI CCGCTC 2 cut(s) 449, 3823
MfeI CAATTG 1 cut(s) 2367
MflI RGATCY 3 cut(s) 1382, 1541, 1973
MhlI GDGCHC 6 cut(s) 588, 991, 1084, 3152, 3421, 3471
MlsI TGGCCA 2 cut(s) 9, 2863
MluNI TGGCCA 2 cut(s) 9, 2863
Mly113I GGCGCC 1 cut(s) 2023
MlyI GAGTC 9 cut(s) 304, 325, 791, 1513, 1798, 2627, 4036, 4279, 4310
MmeI TCCRAC 4 cut(s) 305, 328, 2247, 2844
Mox20I TGGCCA 2 cut(s) 9, 2863
Mph1103I ATGCAT 3 cut(s) 1400, 1534, 2793
MroNI GCCGGC 1 cut(s) 2512
MroXI GAANNNNTTC 2 cut(s) 1819, 3287
MscI TGGCCA 2 cut(s) 9, 2863
MslI CAYNNNNRTG 5 cut(s) 575, 866, 981, 1003, 2796
Msp20I TGGCCA 2 cut(s) 9, 2863
MspA1I CMGCKG 4 cut(s) 1199, 2228, 2312, 4327
MspI CCGG 4 cut(s) 320, 2513, 2631, 3662
MspR9I CCNGG 8 cut(s) 153, 1019, 1520, 2631, 2916, 3405, 3456, 4126
MunI CAATTG 1 cut(s) 2367
Mva1269I GAATGC 3 cut(s) 1064, 1940, 3637
MvaI CCWGG 7 cut(s) 153, 1019, 1520, 2916, 3405, 3456, 4126
MvnI CGCG 1 cut(s) 269
NaeI GCCGGC 1 cut(s) 2514
NarI GGCGCC 1 cut(s) 2023
NciI CCSGG 1 cut(s) 2631
NdeI CATATG 2 cut(s) 2896, 3159
NgoMIV GCCGGC 1 cut(s) 2512
NmuCI GTSAC 9 cut(s) 328, 360, 423, 541, 1655, 2343, 2619, 2975, 4271
NsbI TGCGCA 2 cut(s) 36, 3761
NsiI ATGCAT 3 cut(s) 1400, 1534, 2793
NspI RCATGY 5 cut(s) 361, 2795, 2801, 3185, 3652
NspV TTCGAA 1 cut(s) 561
OliI CACNNNNGTG 1 cut(s) 575
PaeI GCATGC 1 cut(s) 2795
PagI TCATGA 1 cut(s) 1507
PaqCI CACCTGC 3 cut(s) 67, 436, 1874
PasI CCCWGGG 2 cut(s) 1018, 3455
PceI AGGCCT 1 cut(s) 3004
PciI ACATGT 2 cut(s) 357, 3648
PciSI GCTCTTC 1 cut(s) 4097
PctI GAATGC 3 cut(s) 1064, 1940, 3637
PdiI GCCGGC 1 cut(s) 2514
PdmI GAANNNNTTC 2 cut(s) 1819, 3287
PflFI GACNNNGTC 1 cut(s) 1597
PflMI CCANNNNNTGG 3 cut(s) 245, 823, 3680
PfoI TCCNGGA 1 cut(s) 2914
PinAI ACCGGT 1 cut(s) 3661
PleI GAGTC 9 cut(s) 304, 325, 791, 1512, 1797, 2626, 4035, 4278, 4310
PluTI GGCGCC 1 cut(s) 2026
PpsI GAGTC 9 cut(s) 304, 325, 791, 1512, 1797, 2626, 4035, 4278, 4310
PpuMI RGGWCCY 3 cut(s) 155, 2810, 4171
PscI ACATGT 2 cut(s) 357, 3648
PshAI GACNNNNGTC 1 cut(s) 3504
PshBI ATTAAT 4 cut(s) 1067, 1493, 2082, 2388
PsiI TTATAA 1 cut(s) 1683
Psp1406I AACGTT 1 cut(s) 1338
Psp5II RGGWCCY 3 cut(s) 155, 2810, 4171
Psp6I CCWGG 7 cut(s) 151, 1017, 1518, 2914, 3403, 3454, 4124
PspFI CCCAGC 1 cut(s) 1361
PspGI CCWGG 7 cut(s) 151, 1017, 1518, 2914, 3403, 3454, 4124
PspOMI GGGCCC 2 cut(s) 3148, 3467
PspPPI RGGWCCY 3 cut(s) 155, 2810, 4171
PstI CTGCAG 3 cut(s) 139, 373, 1198
PstNI CAGNNNCTG 2 cut(s) 1202, 2921
PsuI RGATCY 3 cut(s) 1382, 1541, 1973
PsyI GACNNNGTC 1 cut(s) 1597
PvuII CAGCTG 1 cut(s) 2228
RsaI GTAC 3 cut(s) 375, 1480, 2704
RsaNI GTAC 3 cut(s) 374, 1479, 2703
RseI CAYNNNNRTG 5 cut(s) 575, 866, 981, 1003, 2796
SapI GCTCTTC 1 cut(s) 4097
ScaI AGTACT 1 cut(s) 1480
SchI GAGTC 9 cut(s) 304, 325, 791, 1513, 1798, 2627, 4036, 4279, 4310
ScrFI CCNGG 8 cut(s) 153, 1019, 1520, 2631, 2916, 3405, 3456, 4126
SduI GDGCHC 6 cut(s) 588, 991, 1084, 3152, 3421, 3471
SfoI GGCGCC 1 cut(s) 2024
SfuI TTCGAA 1 cut(s) 561
SinI GGWCC 6 cut(s) 23, 155, 236, 2810, 3664, 4171
SmiMI CAYNNNNRTG 5 cut(s) 575, 866, 981, 1003, 2796
SmlI CTYRAG 3 cut(s) 405, 1578, 3516
SmoI CTYRAG 3 cut(s) 405, 1578, 3516
SphI GCATGC 1 cut(s) 2795
SseBI AGGCCT 1 cut(s) 3004
SspDI GGCGCC 1 cut(s) 2022
SspMI CTAG 9 cut(s) 639, 1839, 2048, 2061, 2076, 2502, 3369, 3540, 4134
StuI AGGCCT 1 cut(s) 3004
StyD4I CCNGG 8 cut(s) 151, 1017, 1518, 2629, 2914, 3403, 3454, 4124
StyI CCWWGG 2 cut(s) 1452, 2969
TaiI ACGT 3 cut(s) 941, 1341, 3074
TaqI TCGA 8 cut(s) 561, 1154, 1941, 1951, 2380, 2470, 3678, 4017
TaqII GACCGA 2 cut(s) 1749, 2019
TatI WGTACW 1 cut(s) 1478
TauI GCSGC 8 cut(s) 82, 413, 1202, 1423, 1713, 1749, 1755, 4195
TscAI CASTG 8 cut(s) 223, 1660, 1999, 2241, 2332, 2713, 3703, 3895
TseFI GTSAC 9 cut(s) 328, 360, 423, 541, 1655, 2343, 2619, 2975, 4271
Tsp45I GTSAC 9 cut(s) 328, 360, 423, 541, 1655, 2343, 2619, 2975, 4271
TspGWI ACGGA 4 cut(s) 322, 909, 1756, 3117
TspRI CASTG 8 cut(s) 223, 1660, 1999, 2241, 2332, 2713, 3703, 3895
Tth111I GACNNNGTC 1 cut(s) 1597
Van91I CCANNNNNTGG 3 cut(s) 245, 823, 3680
VneI GTGCAC 1 cut(s) 3417
VpaK11BI GGWCC 6 cut(s) 23, 155, 236, 2810, 3664, 4171
VspI ATTAAT 4 cut(s) 1067, 1493, 2082, 2388
XapI RAATTY 5 cut(s) 298, 1445, 3286, 3339, 3543
XbaI TCTAGA 2 cut(s) 2047, 3539
XceI RCATGY 5 cut(s) 361, 2795, 2801, 3185, 3652
XcmI CCANNNNNNNNNTGG 1 cut(s) 17
XmiI GTMKAC 1 cut(s) 1312
XmnI GAANNNNTTC 2 cut(s) 1819, 3287
XspI CTAG 9 cut(s) 639, 1839, 2048, 2061, 2076, 2502, 3369, 3540, 4134
ZrmI AGTACT 1 cut(s) 1480
Zsp2I ATGCAT 3 cut(s) 1400, 1534, 2793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.