Rroxscaffold_3G00270600

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009576
Physical Location & Seq
Forward (+)
63158150 .. 63164436
6287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270600.1

Sequence Viewer

Length: 4422 bp
ATGTATGGCCAAGCTAATTATGGTGCGCCGTTTGGCCAGGGTCAGCCACCGATGCCACCTGCAAACCAACAGGGTCCGCCACCCCCGCCTCCATCTTCCCTTCAGTTCCAACAAGGCTCTCCGGCAGCAATTCCAAATGTAGTTCAGCAGGCTATATCTGCAGTCCCACCTCAGATAGGTCAGCCGGGTCCTTATTTATATCAGCACGGTTCTGTTGCCCCACCTCCCGCTGCTCCGTTCAGTGGGATGCCAAATACAGGTCAATCTTACGGACACGCCAATCCAACAGTCCATGGAAGCACATGGCATAACGTGCAACATGCTTCAGCAGCAGGAAACCAGAATTTCCAACCGATTCATCCACCAGCACTTGTGACTCCTCATGCTCTTCCTTACCCAAACATGTCACAGGCTGCAATACCACCCAGAGCATTACCTCCTCCTCCTCCTCTCCCTTCGACTAATACTACTTCTGGTTGTGCTGCACAAGGACCAACATCGTATAAAGCTTTGTCTCCAGCGCAACCACTGGCCGGTCTTCAGCATGAGGGCTCCCATAATTTTAGAGGTGGAAGTGAGTGTGAAGCAGGTTCTCTAGCTAGAGATGGAGTATCATCAAATGGAAGTGTGATGTTGGATTTTTCTTCACCTCCACCTAAGCCATCAGATGACAAAGTTGATCATAAGATTGATTCTTTCTGCCAGCTTACTGCTATGAATGAAGGGAAAATTGAATCACCATTGAAGTCTTCATGGATTGACTCTTCATCTCAACCTGACCATTTTATCGTCTCTTCAGGTTGTAGCTTGCCTGCAGATTCTGACATGGAGATGGAAGATGATATCACCTTGTCTGACGGAGATCAGGAAGTTCATGATTCATCTAACGCTCTAAACCGCAAGTCTGATGTCAGTCATGGGGAGCTTGATGTCAAAAAGCACCTACATGGAGCACAGAGTTCACCAGGATGGACTGCAGCCCAGGGTGTCTCATGTGAAAATGTGTCATCCTCTTTGTCCCAATGCCAATTGATGATTGAAAAGGCGCCATCTAGAGTTGATATTTATTCAACTGAAGCTTCTGAATCCCCTTTGGCAAGTGAGCGAGATAAAACTTCTATTCCCATAGCTGATGATCAGAACACACTTGATGCTTCTGCAGCGGCTGAGGCAATAAATTCGGACGGGTTTTCTAACCAGATCACCGCAGTTAACAGTCCATTTAGACTTCTACAAGACTATACTTCTGAAAACAGCTCAGAAAATGATCCTCATGCTTTCTTTGAAGATGCTAATGTTGTTATAGCTTCATCATCAGTTACAGCTAGTGTTAAAAGTAGCCATAAAGATGCTGGGCCTCAATTTGAGACAGGATCTAAGAGCTCGTGCATGACTGATAAAATGTCTAGTCTGCAATATGAGTCCAGGAGGTCCAAATTTTCCTTGGATACAAAAAAAGAGGTTAAGGGTACCGGTACAACATTAATCATTGAGAGTCATGAGGCCTTCCAGGGAAAAGAGGCGTTGAGTGGTGCTCCTATTGATATAGTTTCCAAAAGGGATGAGTCTCAGGAGGGGAAAAAGACCAAGTCTGAATCTGTTCTGCCGAAGGTGGATGAGTATGGGAGATTGGTCAGAGAAGGTTCCACTGACAGTAACTCTGATGGTTCACGTTATAATAAGAGGCGTAAAAGAGGCAGAAGCAGAAGCCGCAGTCGCAGCCGTATCCAGAGCCGCAGCCACAGTCGCAGCCGTATGCGGAGCCGGAGCAGGAGCCGCAGTTGTAGCCGGTCCCCTCTTGACAGCAGGAGGAGGAGGAGGAGTCCTCGGAGGAGAAGAGAGAAGCGAAACCGTTCTCCCAGTTGGTCTTCTAGGAATCATAGAAGCAGGAGCAGGTCTCCCACTTTTAGGCGTGCAGGTGATTTCAGAGATGAAAATAAGAGACAGGACAGGCGCCACATTCCAGAATGCTTTGACTTCCTTAGAGGCAGATGCTTCCGTGGAGCATCCTGTCGGTATATGCACCGTGCACATGACCAAAGTGATGGTTCATGGCGCCATAGGAGCAACCAAAAGCATCTAGAAGTTCAGCCTAGTGTGAAGAACTCTAGGATTAAGGAAGAGATTGAAGATAGCTCTGACATGAATCCGCATGGTGAAATCAAAGGTCAAGAGATGCAACAATGTCAAGATGTGGCCGCAAAAGATGGTCAATTTATTGATCCTGATAAAAATGATTGTGAGAGCTCTAGAGTGACAGCTGCTACAATGCAGCTGAAACAAAATTTTCAAGTAAAGTTGGAAGGAACCATCAGTCACAATCCTGAATCTCACCAGCCATCCCCTCAGATGTTGAGCCGTGCTGATAATATGAAGTTGTGGGGTGATACTTCTCAGGCTATTGAACAATTTCGAAGCAATAATTCTGCAGGACAGCTTCAGAAGGCTGATTTTTCGTCTCAGCAGATGGAAGCCTCTCTCATATCTGATTCACCACCAGATCGAGCATCCAAGATTTCTCCAAATAAGGTTTCTAGTATTGAACATGCAACAGATGCACTCTTATCAACTCATTCACGTCCCACTGAATGTTCCAATGCTCAACCACTTTCATCAGGACAGTTTTTATCACAGTATATGGCTCCTAAAGAGTCACCTCTTCCCAGTTTTTCTGCTGCAAATAGTCCATATCCGTCACAGCTGCCTCCTCCTCCTCCTCCTCCTTCACTATCACAAGGTACCAGTGTTGCTCATGTGCCACAGGTGCATAGGGACTACAACCAGAGGCCACCCTATCCAGTTCAGTCCATTCCTACAGGAATGATTCATTCTTATCAAGGTCCCTTACCCAACCAACCTGCCCAATTCTCGGTGTCACAAGATTCACCTTGGACATCCTTGCCGCCGCCACCTCCACGACCACCGTATGATTCATCTTTAAATGCAGGAACTGCTGCGCGAGGTGCTTCTTCACAATTTCAGCAGAGTAACTTGGCTCCAAGGAGTGACTTCAGTTCTCAGAGTTCTATGAGGCCTTACGGCTCTTACCCAACTGAATCCCCTCATTCTAAGGGTGACTTTCTGCATCAGATGTACCCTACATTGTCAGAGCTTCCTCATCCTCTACCGAACAGGGAAGATTTTGGATCAGGCAATCCATCAAATCAACCTTTTGGGGGCCCTGGTCATATGAGAGAAGATCGCTTTACACATGCTCCTGTGCAGGATGTAAATCCTTCACATTCTTTTGCTCAGGGACACCTACACCCTCAACCTGCACCTTCTTCACAGGAGTTAACAAGGATCAAAATGCAAAATTTCTCTGGTGACAATTTTCCATCTGGAGAACTTTTAAATTCATCATCACAAATTCATCCTCATTCACATAACCAACAGCCAAGCTATGGTGTGCAATACTCTGTTGGTGATAGTATTTTGGGTGTGCCTGGGAAGACTGGTGTACAACACCCTGTAGGTGGAAGCATTTTGGGTTTGCCTGGGAAAGATGGCCCCATGTCCCGATACCCTCCAGATATGCTGGACGGGAGTCAGTTTTCTCAAGTTCCTGATTTTGGGGCATCTAGAATTCAAACACATCATAATCCTTACGCAGCTACTTTTGAGCAGCCTCTAAGCTCCAAATTCAGTTCTAACATTCTCATTCAAGAAAAGAATGCACCCGGTGGTAACATGTTTGATACACCGGTCCAAGTCCCAGTTGATGGGCAAGGTGTTGGCAGTGCTGGGTCAAGACAGACAACTTCCTCGCCGAGTTCTGCTAGAGCTGGTGGTCAGCTTTTGCCCAAGTCAGATTGTGAGCAGTATGATCCACTTTTTGACAGCATTGACCCATTAGGAAATCCACTCAATAAACATGATCACAGCCAAAAGCATATACCCACCAGTGATTCGAATATGATGGTGAGGCTCAGTGGTTCTTGTGAGCCATTAGATGTAGAAGAGAACAACAAACATACAGAGGTTCGCCCTGTTGCTTCGGCTACATCTCTGGATAATGACGGCTATGGTGAGACAGCAGATGCAGAAGTGGGTGTTGTTGAGGATGAGAGTGTTAGTAATGATGATGGTGCAGCACACATGGCTGTTGGAGAGATGGAGATTGATCAGGTGAAATCTGGAGGGAAGAGCAAGAAGAAAAAGGATTCCAGATCAACGAGACTTTTCAAGAGTGCTGTTGCAGATTTTGTAAAGGATCTTCTGAAGCCGTCATGGCGGCAGGGTAATATGAGCAAAGAAGCTTTCAAGACCATTGTCAAAAAGACTGTTGATAAGGTTTCTGGAGCCATGAAGAGCCACCAGATACCCAAGTCCGAAGCAAAGATAAATCACTACATTGACTCGTCTCAGCGGAAACTGACAAAGCTTGTGATGGGTTATGTTGATAAGTATGTCAAGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Surp

Protein Analysis

1473

Amino Acids

160.25

Weight (kDa)

7.36

Isoelectric Point (pI)

68.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAF11-like_C PF23030 1389 - 1457 3.8e-06 SCAF11-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1677
AarI CACCTGC 2 cut(s) 67, 1907
Acc36I ACCTGC 6 cut(s) 67, 578, 1884, 1907, 2866, 3283
Acc65I GGTACC 2 cut(s) 1469, 2738
AccB1I GGYRCC 5 cut(s) 1045, 1469, 1953, 2055, 2738
AccB7I CCANNNNNTGG 2 cut(s) 3404, 3722
AccII CGCG 1 cut(s) 2959
AclWI GGATC 7 cut(s) 1262, 1381, 2216, 3154, 3311, 3821, 4221
AcoI YGGCCR 4 cut(s) 7, 34, 531, 2196
AcsI RAATTY 9 cut(s) 343, 1177, 1436, 2284, 3316, 3355, 3369, 3585, 3641
AcuI CTGAAG 8 cut(s) 86, 309, 524, 780, 1095, 2423, 2995, 4241
AcyI GRCGYC 3 cut(s) 1046, 1954, 2056
AdeI CACNNNGTG 1 cut(s) 3683
AfaI GTAC 5 cut(s) 1471, 1477, 2740, 3095, 3462
AflIII ACRYGT 2 cut(s) 402, 3690
AgeI ACCGGT 2 cut(s) 1472, 3703
AjiI CACGTC 1 cut(s) 2579
AjnI CCWGG 8 cut(s) 36, 964, 981, 1424, 1509, 3181, 3445, 3496
AjuI GAANNNNNNNTTGG 2 cut(s) 2515, 2547
Alw21I GWGCWC 5 cut(s) 955, 1385, 1537, 2032, 2249
Alw44I GTGCAC 1 cut(s) 2028
AlwI GGATC 7 cut(s) 1262, 1381, 2216, 3154, 3311, 3821, 4221
AlwNI CAGNNNCTG 3 cut(s) 821, 1166, 2951
ApaI GGGCCC 1 cut(s) 3182
ApaLI GTGCAC 1 cut(s) 2028
ApoI RAATTY 9 cut(s) 343, 1177, 1436, 2284, 3316, 3355, 3369, 3585, 3641
AseI ATTAAT 1 cut(s) 1484
AsiGI ACCGGT 2 cut(s) 1472, 3703
Asp700I GAANNNNTTC 3 cut(s) 1599, 1852, 2409
Asp718I GGTACC 2 cut(s) 1469, 2738
AspLEI GCGC 6 cut(s) 28, 523, 1048, 1956, 2058, 2959
AsuC2I CCSGG 2 cut(s) 186, 3681
AsuII TTCGAA 2 cut(s) 2413, 3911
AvaII GGWCC 7 cut(s) 74, 188, 491, 1431, 1791, 2840, 3706
BaeGI GKGCMC 2 cut(s) 2032, 3182
BaeI ACNNNNGTAYC 6 cut(s) 2730, 2763, 3690, 3723, 4313, 4346
BalI TGGCCA 2 cut(s) 9, 36
BanI GGYRCC 5 cut(s) 1045, 1469, 1953, 2055, 2738
BanII GRGCYC 4 cut(s) 554, 1385, 2249, 3182
BarI GAAGNNNNNNTAC 2 cut(s) 4200, 4232
BauI CACGAG 1 cut(s) 1384
BbsI GAAGAC 4 cut(s) 530, 741, 1860, 3458
Bbv12I GWGCWC 5 cut(s) 955, 1385, 1537, 2032, 2249
BbvCI CCTCAGC 1 cut(s) 1167
BceAI ACGGC 7 cut(s) 13, 1707, 1737, 2343, 3055, 4036, 4210
BcgI CGANNNNNNTGC 2 cut(s) 1161, 1195
BciT130I CCWGG 8 cut(s) 38, 966, 983, 1426, 1511, 3183, 3447, 3498
BciVI GTATCC 2 cut(s) 1441, 1736
BclI TGATCA 4 cut(s) 679, 1135, 3877, 4123
BcnI CCSGG 2 cut(s) 186, 3681
BfmI CTRYAG 7 cut(s) 159, 813, 975, 1158, 2427, 2814, 3471
BfoI RGCGCY 3 cut(s) 1049, 1957, 2059
BfuAI ACCTGC 6 cut(s) 67, 578, 1884, 1907, 2866, 3283
BfuI GTATCC 2 cut(s) 1441, 1736
BglI GCCNNNNNGGC 1 cut(s) 4231
Bme18I GGWCC 7 cut(s) 74, 188, 491, 1431, 1791, 2840, 3706
BmgBI CACGTC 1 cut(s) 2579
BmrI ACTGGG 3 cut(s) 1854, 2658, 3710
BmuI ACTGGG 3 cut(s) 1854, 2658, 3710
BoxI GACNNNNGTC 2 cut(s) 3546, 4271
BpiI GAAGAC 4 cut(s) 530, 741, 1860, 3458
BplI GAGNNNNNCTC 6 cut(s) 1519, 1551, 1810, 1842, 1882, 1914
BpmI CTGGAG 5 cut(s) 501, 3363, 3513, 4158, 4320
Bpu10I CCTNAGC 3 cut(s) 657, 1167, 3252
Bpu14I TTCGAA 2 cut(s) 2413, 3911
BpuEI CTTGAG 1 cut(s) 3543
BpuMI CCSGG 2 cut(s) 186, 3681
BsaBI GATNNNNATC 1 cut(s) 3231
BsaHI GRCGYC 3 cut(s) 1046, 1954, 2056
BsaI GGTCTC 1 cut(s) 1902
BsaWI WCCGGW 2 cut(s) 1472, 3703
BsaXI ACNNNNNCTCC 6 cut(s) 1765, 1795, 1807, 1837, 3199, 3229
Bse118I RCCGGY 4 cut(s) 533, 1472, 1788, 3703
Bse1I ACTGG 8 cut(s) 534, 1860, 2664, 2742, 2798, 3460, 3716, 3903
Bse8I GATNNNNATC 1 cut(s) 3231
BseBI CCWGG 8 cut(s) 38, 966, 983, 1426, 1511, 3183, 3447, 3498
BseJI GATNNNNATC 1 cut(s) 3231
BseNI ACTGG 8 cut(s) 534, 1860, 2664, 2742, 2798, 3460, 3716, 3903
BseSI GKGCMC 2 cut(s) 2032, 3182
BseYI CCCAGC 2 cut(s) 1352, 3743
BsgI GTGCAG 5 cut(s) 468, 1935, 3242, 3261, 4110
Bsh1236I CGCG 1 cut(s) 2959
BshNI GGYRCC 5 cut(s) 1045, 1469, 1953, 2055, 2738
BshTI ACCGGT 2 cut(s) 1472, 3703
BsiHKAI GWGCWC 5 cut(s) 955, 1385, 1537, 2032, 2249
BsiSI CCGG 8 cut(s) 122, 185, 534, 1473, 1765, 1789, 3681, 3704
BslFI GGGAC 9 cut(s) 149, 1003, 1777, 2565, 2786, 2826, 3270, 3502, 3698
BsmBI CGTCTC 3 cut(s) 796, 2463, 4368
BsmFI GGGAC 9 cut(s) 149, 1003, 1777, 2565, 2786, 2826, 3270, 3502, 3698
BsmI GAATGC 2 cut(s) 1973, 3679
Bso31I GGTCTC 1 cut(s) 1902
Bsp119I TTCGAA 2 cut(s) 2413, 3911
Bsp120I GGGCCC 1 cut(s) 3178
Bsp1286I GDGCHC 7 cut(s) 554, 955, 1385, 1537, 2032, 2249, 3182
Bsp1407I TGTACA 1 cut(s) 3460
Bsp19I CCATGG 1 cut(s) 292
BspFNI CGCG 1 cut(s) 2959
BspHI TCATGA 2 cut(s) 874, 1498
BspMAI CTGCAG 5 cut(s) 163, 817, 979, 1162, 2431
BspMI ACCTGC 6 cut(s) 67, 578, 1884, 1907, 2866, 3283
BspPI GGATC 7 cut(s) 1262, 1381, 2216, 3154, 3311, 3821, 4221
BspQI GCTCTTC 3 cut(s) 393, 4139, 4304
BspT104I TTCGAA 2 cut(s) 2413, 3911
BspT107I GGYRCC 5 cut(s) 1045, 1469, 1953, 2055, 2738
BspTNI GGTCTC 1 cut(s) 1902
BsrFI RCCGGY 4 cut(s) 533, 1472, 1788, 3703
BsrGI TGTACA 1 cut(s) 3460
BsrI ACTGG 8 cut(s) 534, 1860, 2664, 2742, 2798, 3460, 3716, 3903
BssAI RCCGGY 4 cut(s) 533, 1472, 1788, 3703
BssNI GRCGYC 3 cut(s) 1046, 1954, 2056
BssSI CACGAG 1 cut(s) 1384
BssT1I CCWWGG 4 cut(s) 292, 1443, 2888, 2999
Bst2BI CACGAG 1 cut(s) 1384
Bst2UI CCWGG 8 cut(s) 38, 966, 983, 1426, 1511, 3183, 3447, 3498
Bst6I CTCTTC 9 cut(s) 393, 769, 799, 1831, 2115, 2664, 3954, 4139, 4304
BstACI GRCGYC 3 cut(s) 1046, 1954, 2056
BstAPI GCANNNNNTGC 3 cut(s) 313, 2555, 2951
BstAUI TGTACA 1 cut(s) 3460
BstBI TTCGAA 2 cut(s) 2413, 3911
BstC8I GCNNGC 5 cut(s) 150, 704, 809, 813, 1914
BstDSI CCRYGG 2 cut(s) 292, 1999
BstENI CCTNNNNNAGG 1 cut(s) 174
BstFNI CGCG 1 cut(s) 2959
BstH2I RGCGCY 3 cut(s) 1049, 1957, 2059
BstHHI GCGC 6 cut(s) 28, 523, 1048, 1956, 2058, 2959
BstNI CCWGG 8 cut(s) 38, 966, 983, 1426, 1511, 3183, 3447, 3498
BstNSI RCATGY 5 cut(s) 323, 406, 2549, 3215, 3694
BstPAI GACNNNNGTC 2 cut(s) 3546, 4271
BstSFI CTRYAG 7 cut(s) 159, 813, 975, 1158, 2427, 2814, 3471
BstSLI GKGCMC 2 cut(s) 2032, 3182
BstUI CGCG 1 cut(s) 2959
BstV2I GAAGAC 4 cut(s) 530, 741, 1860, 3458
BstX2I RGATCY 2 cut(s) 1373, 4213
BstXI CCANNNNNNTGG 1 cut(s) 2045
BstYI RGATCY 2 cut(s) 1373, 4213
BsuI GTATCC 2 cut(s) 1441, 1736
BtgI CCRYGG 2 cut(s) 292, 1999
BtrI CACGTC 1 cut(s) 2579
BtsI GCAGTG 1 cut(s) 3745
BtsIMutI CAGTG 8 cut(s) 247, 527, 1647, 2583, 2749, 3745, 3910, 3937
BveI ACCTGC 6 cut(s) 67, 578, 1884, 1907, 2866, 3283
Cac8I GCNNGC 5 cut(s) 150, 704, 809, 813, 1914
CaiI CAGNNNCTG 3 cut(s) 821, 1166, 2951
CciI TCATGA 2 cut(s) 874, 1498
CfoI GCGC 6 cut(s) 28, 523, 1048, 1956, 2058, 2959
Cfr10I RCCGGY 4 cut(s) 533, 1472, 1788, 3703
Csp6I GTAC 5 cut(s) 1470, 1476, 2739, 3094, 3461
CspAI ACCGGT 2 cut(s) 1472, 3703
CviQI GTAC 5 cut(s) 1470, 1476, 2739, 3094, 3461
DinI GGCGCC 3 cut(s) 1047, 1955, 2057
DraI TTTAAA 2 cut(s) 2940, 3354
DraIII CACNNNGTG 1 cut(s) 3683
EaeI YGGCCR 4 cut(s) 7, 34, 531, 2196
Eam1104I CTCTTC 9 cut(s) 393, 769, 799, 1831, 2115, 2664, 3954, 4139, 4304
EarI CTCTTC 9 cut(s) 393, 769, 799, 1831, 2115, 2664, 3954, 4139, 4304
EciI GGCGGA 1 cut(s) 66
Ecl136II GAGCTC 2 cut(s) 1383, 2247
Eco130I CCWWGG 4 cut(s) 292, 1443, 2888, 2999
Eco147I AGGCCT 2 cut(s) 1505, 3034
Eco24I GRGCYC 4 cut(s) 554, 1385, 2249, 3182
Eco31I GGTCTC 1 cut(s) 1902
Eco32I GATATC 1 cut(s) 844
Eco47I GGWCC 7 cut(s) 74, 188, 491, 1431, 1791, 2840, 3706
Eco53kI GAGCTC 2 cut(s) 1383, 2247
Eco57I CTGAAG 8 cut(s) 86, 309, 524, 780, 1095, 2423, 2995, 4241
EcoICRI GAGCTC 2 cut(s) 1383, 2247
EcoNI CCTNNNNNAGG 1 cut(s) 174
EcoO109I RGGNCCY 4 cut(s) 188, 2840, 3178, 3179
EcoRI GAATTC 1 cut(s) 3585
EcoRII CCWGG 8 cut(s) 36, 964, 981, 1424, 1509, 3181, 3445, 3496
EcoRV GATATC 1 cut(s) 844
EcoT14I CCWWGG 4 cut(s) 292, 1443, 2888, 2999
EcoT38I GRGCYC 4 cut(s) 554, 1385, 2249, 3182
EgeI GGCGCC 3 cut(s) 1047, 1955, 2057
EheI GGCGCC 3 cut(s) 1047, 1955, 2057
ErhI CCWWGG 4 cut(s) 292, 1443, 2888, 2999
Esp3I CGTCTC 3 cut(s) 796, 2463, 4368
FalI AAGNNNNNCTT 2 cut(s) 3062, 3094
FaqI GGGAC 9 cut(s) 149, 1003, 1777, 2565, 2786, 2826, 3270, 3502, 3698
FauI CCCGC 2 cut(s) 93, 235
FauNDI CATATG 1 cut(s) 3189
FbaI TGATCA 4 cut(s) 679, 1135, 3877, 4123
FriOI GRGCYC 4 cut(s) 554, 1385, 2249, 3182
GlaI GCGC 6 cut(s) 27, 522, 1047, 1955, 2057, 2958
GsaI CCCAGC 2 cut(s) 1356, 3747
GsuI CTGGAG 5 cut(s) 501, 3363, 3513, 4158, 4320
HaeII RGCGCY 3 cut(s) 1049, 1957, 2059
HapII CCGG 8 cut(s) 122, 185, 534, 1473, 1765, 1789, 3681, 3704
HhaI GCGC 6 cut(s) 28, 523, 1048, 1956, 2058, 2959
Hin1I GRCGYC 3 cut(s) 1046, 1954, 2056
Hin6I GCGC 6 cut(s) 26, 521, 1046, 1954, 2056, 2957
HinP1I GCGC 6 cut(s) 26, 521, 1046, 1954, 2056, 2957
HincII GTYRAC 2 cut(s) 1213, 3297
HindII GTYRAC 2 cut(s) 1213, 3297
HindIII AAGCTT 4 cut(s) 507, 1077, 4257, 4382
HpaI GTTAAC 2 cut(s) 1213, 3297
HpaII CCGG 8 cut(s) 122, 185, 534, 1473, 1765, 1789, 3681, 3704
Hpy166II GTNNAC 6 cut(s) 962, 1213, 1670, 2030, 3297, 3461
Hpy8I GTNNAC 6 cut(s) 962, 1213, 1670, 2030, 3297, 3461
HpyCH4IV ACGT 3 cut(s) 312, 1672, 2578
HpySE526I ACGT 3 cut(s) 312, 1672, 2578
Hsp92I GRCGYC 3 cut(s) 1046, 1954, 2056
HspAI GCGC 6 cut(s) 26, 521, 1046, 1954, 2056, 2957
KasI GGCGCC 3 cut(s) 1045, 1953, 2055
KpnI GGTACC 2 cut(s) 1473, 2742
Ksp22I TGATCA 4 cut(s) 679, 1135, 3877, 4123
KspAI GTTAAC 2 cut(s) 1213, 3297
LguI GCTCTTC 3 cut(s) 393, 4139, 4304
MaeII ACGT 3 cut(s) 312, 1672, 2578
MfeI CAATTG 1 cut(s) 1028
MflI RGATCY 2 cut(s) 1373, 4213
MhlI GDGCHC 7 cut(s) 554, 955, 1385, 1537, 2032, 2249, 3182
MlsI TGGCCA 2 cut(s) 9, 36
MluNI TGGCCA 2 cut(s) 9, 36
Mly113I GGCGCC 3 cut(s) 1046, 1954, 2056
MlyI GAGTC 9 cut(s) 370, 755, 1430, 1504, 1574, 1831, 2660, 3556, 4352
MmeI TCCRAC 6 cut(s) 133, 308, 373, 615, 2280, 4087
Mox20I TGGCCA 2 cut(s) 9, 36
MroXI GAANNNNTTC 3 cut(s) 1599, 1852, 2409
MscI TGGCCA 2 cut(s) 9, 36
MseI TTAA 8 cut(s) 1212, 1332, 1464, 1484, 2115, 2939, 3296, 3353
MslI CAYNNNNRTG 4 cut(s) 830, 945, 967, 1347
Msp20I TGGCCA 2 cut(s) 9, 36
MspA1I CMGCKG 6 cut(s) 230, 1163, 2261, 2275, 2701, 4369
MspI CCGG 8 cut(s) 122, 185, 534, 1473, 1765, 1789, 3681, 3704
MunI CAATTG 1 cut(s) 1028
Mva1269I GAATGC 2 cut(s) 1973, 3679
MvaI CCWGG 8 cut(s) 38, 966, 983, 1426, 1511, 3183, 3447, 3498
MvnI CGCG 1 cut(s) 2959
NarI GGCGCC 3 cut(s) 1046, 1954, 2056
NciI CCSGG 2 cut(s) 186, 3681
NcoI CCATGG 1 cut(s) 292
NdeI CATATG 1 cut(s) 3189
NmeAIII GCCGAG 1 cut(s) 3795
NspI RCATGY 5 cut(s) 323, 406, 2549, 3215, 3694
NspV TTCGAA 2 cut(s) 2413, 3911
PagI TCATGA 2 cut(s) 874, 1498
PaqCI CACCTGC 2 cut(s) 67, 1907
PasI CCCWGGG 1 cut(s) 982
PceI AGGCCT 2 cut(s) 1505, 3034
PciI ACATGT 2 cut(s) 402, 3690
PciSI GCTCTTC 3 cut(s) 393, 4139, 4304
PctI GAATGC 2 cut(s) 1973, 3679
PdmI GAANNNNTTC 3 cut(s) 1599, 1852, 2409
PflFI GACNNNGTC 1 cut(s) 1588
PflMI CCANNNNNTGG 2 cut(s) 3404, 3722
PfoI TCCNGGA 1 cut(s) 1424
PinAI ACCGGT 2 cut(s) 1472, 3703
PleI GAGTC 9 cut(s) 370, 755, 1429, 1503, 1573, 1830, 2659, 3555, 4352
PluTI GGCGCC 3 cut(s) 1049, 1957, 2059
PpsI GAGTC 9 cut(s) 370, 755, 1429, 1503, 1573, 1830, 2659, 3555, 4352
PpuMI RGGWCCY 2 cut(s) 188, 2840
PscI ACATGT 2 cut(s) 402, 3690
PshAI GACNNNNGTC 2 cut(s) 3546, 4271
PshBI ATTAAT 1 cut(s) 1484
PsiI TTATAA 1 cut(s) 1677
Psp124BI GAGCTC 2 cut(s) 1385, 2249
Psp5II RGGWCCY 2 cut(s) 188, 2840
Psp6I CCWGG 8 cut(s) 36, 964, 981, 1424, 1509, 3181, 3445, 3496
PspFI CCCAGC 2 cut(s) 1352, 3743
PspGI CCWGG 8 cut(s) 36, 964, 981, 1424, 1509, 3181, 3445, 3496
PspOMI GGGCCC 1 cut(s) 3178
PspPPI RGGWCCY 2 cut(s) 188, 2840
PstI CTGCAG 5 cut(s) 163, 817, 979, 1162, 2431
PstNI CAGNNNCTG 3 cut(s) 821, 1166, 2951
PsuI RGATCY 2 cut(s) 1373, 4213
PsyI GACNNNGTC 1 cut(s) 1588
PvuII CAGCTG 3 cut(s) 2261, 2275, 2701
RsaI GTAC 5 cut(s) 1471, 1477, 2740, 3095, 3462
RsaNI GTAC 5 cut(s) 1470, 1476, 2739, 3094, 3461
RseI CAYNNNNRTG 4 cut(s) 830, 945, 967, 1347
SacI GAGCTC 2 cut(s) 1385, 2249
SapI GCTCTTC 3 cut(s) 393, 4139, 4304
SaqAI TTAA 8 cut(s) 1212, 1332, 1464, 1484, 2115, 2939, 3296, 3353
SchI GAGTC 9 cut(s) 370, 755, 1430, 1504, 1574, 1831, 2660, 3556, 4352
SduI GDGCHC 7 cut(s) 554, 955, 1385, 1537, 2032, 2249, 3182
SfcI CTRYAG 7 cut(s) 159, 813, 975, 1158, 2427, 2814, 3471
SfoI GGCGCC 3 cut(s) 1047, 1955, 2057
SfuI TTCGAA 2 cut(s) 2413, 3911
SinI GGWCC 7 cut(s) 74, 188, 491, 1431, 1791, 2840, 3706
SmiMI CAYNNNNRTG 4 cut(s) 830, 945, 967, 1347
SmlI CTYRAG 1 cut(s) 3558
SmoI CTYRAG 1 cut(s) 3558
SseBI AGGCCT 2 cut(s) 1505, 3034
SspDI GGCGCC 3 cut(s) 1045, 1953, 2055
SstI GAGCTC 2 cut(s) 1385, 2249
StuI AGGCCT 2 cut(s) 1505, 3034
StyI CCWWGG 4 cut(s) 292, 1443, 2888, 2999
TaiI ACGT 3 cut(s) 315, 1675, 2581
TaqI TCGA 4 cut(s) 458, 2413, 2503, 3911
TatI WGTACW 1 cut(s) 3460
TauI GCSGC 8 cut(s) 1166, 1713, 1737, 1779, 2201, 2905, 2908, 4237
Tru1I TTAA 8 cut(s) 1212, 1332, 1464, 1484, 2115, 2939, 3296, 3353
Tru9I TTAA 8 cut(s) 1212, 1332, 1464, 1484, 2115, 2939, 3296, 3353
TscAI CASTG 8 cut(s) 247, 534, 1654, 2590, 2749, 3745, 3910, 3937
TspGWI ACGGA 5 cut(s) 225, 285, 873, 1988, 2682
TspRI CASTG 8 cut(s) 247, 534, 1654, 2590, 2749, 3745, 3910, 3937
Tth111I GACNNNGTC 1 cut(s) 1588
Van91I CCANNNNNTGG 2 cut(s) 3404, 3722
VneI GTGCAC 1 cut(s) 2028
VpaK11BI GGWCC 7 cut(s) 74, 188, 491, 1431, 1791, 2840, 3706
VspI ATTAAT 1 cut(s) 1484
XagI CCTNNNNNAGG 1 cut(s) 174
XapI RAATTY 9 cut(s) 343, 1177, 1436, 2284, 3316, 3355, 3369, 3585, 3641
XbaI TCTAGA 4 cut(s) 1052, 2080, 2249, 3581
XceI RCATGY 5 cut(s) 323, 406, 2549, 3215, 3694
XcmI CCANNNNNNNNNTGG 3 cut(s) 17, 1349, 1441
XmnI GAANNNNTTC 3 cut(s) 1599, 1852, 2409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.