FvH4_5g14340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006932
Physical Location & Seq
Reverse (-)
8102878 .. 8110828
7951 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14340.t1

Sequence Viewer

Length: 5028 bp
ATGGAGAACAGTGGAGAAGCTCTAGATGATGATGGCTCTGGATGGTTTGAAGTGAAAAAGAAACACAGAAGTAGCTCAAAGTTGTCTCTACAGAGTTGGGTTGGGGGAAGTTCTGCAAAGAATGCTAACTGTTCGAGTAGTCATCCTTTATCAAGTGAAAACAGTAGGAATTATTCTGGCAAACGCAGATCCCAGCTTCCAAAGGTAAGGGAAAATAGTGCTGTTCAAAGGCAGGGAAGTGATGCAGGTTCCACTCCCAAGCCAGATAAGAGTGAAACGGTTGTTCCCTGTGATATTGGCATCAACAAGCAAGGTGCTAAATGTCCAATGTCACCTCCATTTATTACAAATCCTGATGGTGAAACTAGAGATTCAGAGGAGAACCCTGCAAGTGATAACTCTGAAGTGGTTCATAAAATTAAGTGGGGCGATCTAGAGGATGAGAGTTTGGCACTTCCTCATACTAACCTTGTCGGCACCAGAATCAAGTTTGGTGCTATTGGAGATGAAAATCTGATGGCCTCTAAAGAGCACGAGAATTGTCATAGTTTTGTTCCTTCCGCAAATGCTCAAGAAAAAGAACTGCTTGCAGCAACTGCAGATGCTAATATTGTTTCTCATCAGACGGCTCCAGTGAATACTAATGATCAATTTTATGAAGATAACTGCAAGGAGGTGAATGTTATATCTGCTGAAAATGTTGTGGACCCGATTCTGAATGACAAAATGGTTGATGTGGATAACAGTACTTTAAATTGCAAGGATGTACATACTGAAAAAATTGAAGCAGTCACTGATGTTCCTGTAAGTGCTAGCACTTTATCAGTGGGGAAAGTTGAAGCTCCAGTGGTTGTAACTGAGGTACGTGATCCTGCAATTTTTGAAGAATCAGGACGTCATGGCAGTTCAAGTGAAGTTCATATCTCCAAAGATAATGACTTAGATACACCAGAGAGCGACCCTGAAATTTGTGCAGAGCCTACGCTCACAGCATCTGGACATTATATATCAAACTCAAATATGAGTGCCCTTGGAGATTGTGACACAGGTGAAAGCAAAGAAAGGTTTAGGCAGCGACTTTGGTGCTATCTCTTTGAGAATCTTAACCGAGCTGTGGATGAGCTTTATCTTCTTTGTGAACTAGAGTGCGATGTAGAGCAGATGAAAGAGGCAATTCTTGTTCTTGAAGAGGCTCGCTCTGATTTCAGAGATTTGAACACTAGAGTGGAAGATTTTGAGAAAATAAAAAAGGCACCATCTCAGTTGATTGATGGGGTGCCCATCACCTTGAAAAGTGACCATCGGAGGCCACATGCTCTTTCTTGGGAGGTTAGAAGAATGACAACTTCCGCACACAAAGCAGAGATACTGTCCTCATCTCTTGAGGCTTTTAAGAAAATTCAGAAAGAAAGAGCTAGTGCAGCAAATGACGCTCAACTTATGGGCCTTAAGTATACAAATATCCAATCTAGCGATAACCTCAATAAGTCACCTGCAAGATATGACGTGAAATTCAATTCTAAAGAATCAACAATGAAGTCAAGAAGACATAGTGGAGGTTCAAATCTTGTTGAAGCTGTCCTGAATGGGAATCAGAATACTGAACCAAGCAGTTCGAGCAGAGTAAAGTTGGTACAAAATGGGCGCCTCTCTCAGAATTCATCTGCCTTTGTTGTTAATGCATCTAGATTGCCTCCTAGGGATAATTCTGCTGCTGGAAAAACTAAGAGAGAACAGTCTGGCTCTATGTCTGAATCCGAGAAGCTGCTTGCTAGGAAAGACAAACTGTCAACAGAATGTGGTGTTGAAAAAATTGCCAAGTTGACTGATCAGTCCAAAAGGCAAATTCCACTTTTGGAAAAAGATAAAGAAAAGAGGAACTCAGCTCCATGGAAATCCATGGATGCTTGGAAGGAGAAGAGGAATTGGGAGGATGTTCTTTCATCTCCTTCTCGTGTGTCTTCCAGGGTGTCACATTCACCTGGCATGAGGAGGAAAAGTGCTGATCGTGCACGAATGTTGCATGATAAACTAATGTCCCCTGAGAAGAAGAAAAAAACTAGTTTGGATCTGAAGAGAGAGGCAGAAGAAAAGCATGCCCGTGCTATGAGAATCAGAAGCGAGCTAGAGAATGAGAGAGCTCAGAAGCTTCATCGTAGCTCTGAAAAGATGAATAGAGTTAATGAATTGCAGGCTGTCAAAAATATGAAGTTACGGGAGGGAATGCATGCTCGTCATCAACGCAGTGAGTCTCGACATGAAGCACATCTAGCTCAACGAGTGAAGAGAGCTGGTGATGAAAGCATCAAAGTCAAAGAGGTTCAGTTCATAACTTCCTTAAACGAAGAAAATAAAAAGCTCAGTTTGCGCCAGAAGCATCATGATTCAGAACTGAGGAGAGCTGAAAAACTTCAAGTGATAAGAACTAAACAAAAAGAGGATATGGCGCGAGAAGAAGCTGTTTTAGAGCGGAGGAAACTCATTGAAGCTGAAAAATTGCAGCGTCTTGCTGAAACACAGCGGAGAAAGGAAGAGGCTCAAGTTAGAAGGGAGGAGGAACGTAAAGCATCAAGTGCAGCACGTGAAGCAAGGGCCATCGAACAACTTCGGAGGAAGGAGGAAAGAGCCAAAGCCCAGCAGGAGGAGGCTGAACTGCTGGCCCAGAAATTGGCAGAGAGATTGCGTGAAAGTGAGCAGCGCCGAAAATTTTATCTGGAGCAGATACGAGAGAGAGCTTCTATGGATTTCAGGGATCAATCTTCACCTTTATTGCGTCGAACTTTAAACAAGGACGTTCAGGGAAGATCATCATCAATTAACAATGGTGATGATTATCAAGTAAGCAGTTTCTCAGGCTTAGGAAGTAGCACTTTTGCGGAAAGCAATAATACAGCACAACATTCAGTGAAGCGGAGGATTAAAAAAATTCGACAGAGACTTATGGCTCTAAAATATGAAATTCTTGAGCCTCCTGTTGGTGCTGAAAATGCTGGCATTGGATACAGAACGGCACTGGGAACTGCAAGGGCAAAGATTGGGAGATGGCTTCAAGAACTTCAAAGACTCCGACAAGCAAGAAAAGAAGGAGCTGCAAGCATTGGGTTAATAACAGCTGAAATGATCAAGTATTTGGAGGGAAAGGAGCTGGAACTGCAGGCTTCCCGGCAAGCTGGTCTGATTGATTTTATAGCATCTGCCCTGCCTGCTTCTCATACATCAAAACCTGAAGCCTGTCAGGTGACAATACATCTTTTAAAACTCTTGAGGGTGGTGTTATCTCTCCCTACAAACAGGAGTTATTTTCTTGCACAAAATCTCTTGCCCCCGATCATCCCAATGCTGTCTGCTTCCCTTGAGAGCTACATAAAGATAGCAGTATCCTTGAATCCTTCTGGTAATGTTAACTTCCCATCTACCAAAACCTCAGCTGAAAATTTTGAATCAATATCTGAGGTCTTGGATGGTTATTTATGGACTGTAACAACAATTCTCAGCCACATAAGTTCTGATGAACGACAGCTTCAAATGAGGGATAGTTTGTTGGAGCTGCTGATTTCCTACCAAGTTATTCAGCGGCTGCGAGATCTGTTTGCCCTTTATGATCGACCCCAGGTGGAAGGGTCACCATTTCCATCGTCAATTATCTTAAGTATACGTTTGTTGGTTGTCTTAACTTCCAGGTCTGAAACTGATTGTTCTATTGATTGGAAATACGAACCTGTTGAAATACTGCTTGGAAATGGAAGTGAAGAGGCTAAGGTTGCTGAGTGTGATAATTCGGAATATCTTCCTCCAACTCTGACCTTGGAAGATTTTAGACCTCCATCATCTTTGTTGAATGGTGGTAAATTTGTACATCTACCAGATGTACCAAAGGATGGACCAGTAGATGAGATGTGTAAGATAAATGAATCTGTGGAGTCAGTATCTGCTGCTAAGGGTAGTGAGGAGCGTAATAGTCTGGTTGAAGCAAACAATGCTAACAAAGTCAAAACAGATGTTCCAGATGAACCCCAGAAGATGGTGAATGATGATATCATGGAGCCTTTTGCATCAGTGGAGGAAGAGAAACACTTGGTGGATAATGGTGCAGAACATAAGAATGATAACTGTGTGACCTTGCAGCAACCAGTAGCATTTCTTCTATCAGCTGTATCAGAAACAGGGCTTGTCAGTCTCCCTTCTCTGTTGACATCTGTGCTATTACAGGCAAACAATAGATTGTCTTCTGAACAGGCCTCAGATGCCCTCCCATCTAATTTTGAAGATGTAGCAACTGGAGTGCTGAAGGTGTTGAACAATTTGGCTCTTCTGGATCTGAAGTTCATGCAGAGAATGCTAGCTAGGCCAGACCTAAAAATGGAATTTTTCCATTTAATGAGTTTCCTGCTTTCTCATTGTACAAGCAAGTGGAAAGTAGCTAATGATCCGGTGGGGCTGCTTTTGCTTGAATCTCTTTTGCTCCTTGGTCACTTTGCCCTGTTCCACCTTGGGAATCAAGCTGTCCTTCGTTGGGGAAAGAGTCCTACCATTATTCACAAGGTATGCGATTTGCCATTTGTGTTCTTCAGTGATCCTGAGTTGATGCCAGTCTTGGCTGGCACACTGGTTGCTGCCTGTTACGGGTGTGAGCAGAACAAGGGTGTGGTTCAGCAGGAAATGAGTACAGATATGCTACTCTCCCTGTTGCGATCTTGCAGAAACGTCTTACCAGCAGTTAGATCCAATTCTAATGTGGACAGTTGCCCAGCAGATGATGTGCCCCTAAGATCATGCCGTAACAATAACAAGAACTATAGGGTTTCTTCCGGAAAGGGTGTTGCTTCTGGAAACAGCATGAGAAATGGCAAGATGAGAAGCCATAGGGAAAGTAAAATGATGAAGACTTATGAAGAGTTGGCTCCGAAGCAGATTCTTCCATCGTCAGAAACTGCCTCTATGATGCTGCATTGTAGATTTCCTATCAGCTTCATTGATAGAGCAGAAAATTTCTTTTCTACTGAGAACCCTAGCATCGTCGGAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAPER_N

Protein Analysis

1676

Amino Acids

187.43

Weight (kDa)

7.24

Isoelectric Point (pI)

56.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAPER_N PF16501 351 - 451 6.5e-16 S phase cyclin A-associated protein in the endoplasmic reticulum
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1501
AasI GACNNNNNNGTC 1 cut(s) 1831
AatII GACGTC 1 cut(s) 898
Acc36I ACCTGC 2 cut(s) 236, 1501
AccB1I GGYRCC 4 cut(s) 476, 1252, 1276, 1644
AccB7I CCANNNNNTGG 3 cut(s) 2668, 3878, 4021
AccBSI CCGCTC 1 cut(s) 2470
AccI GTMKAC 2 cut(s) 1454, 3652
AccII CGCG 1 cut(s) 2449
AccIII TCCGGA 1 cut(s) 4808
AciI CCGC 7 cut(s) 561, 1350, 2470, 2521, 2876, 2911, 3574
AclWI GGATC 8 cut(s) 183, 863, 2076, 2760, 4323, 4421, 4568, 4716
AcuI CTGAAG 6 cut(s) 423, 2093, 3246, 4307, 4340, 4552
AcvI CACGTG 1 cut(s) 2582
AcyI GRCGYC 2 cut(s) 895, 1645
AdeI CACNNNGTG 1 cut(s) 4078
AfaI GTAC 8 cut(s) 748, 768, 864, 1635, 3855, 3870, 4402, 4666
AfiI CCNNNNNNNGG 9 cut(s) 1114, 2641, 2668, 2975, 3878, 4021, 4360, 4513, 4623
AflII CTTAAG 2 cut(s) 1448, 3646
AhdI GACNNNNNGTC 1 cut(s) 1786
AhlI ACTAGT 1 cut(s) 2060
AjiI CACGTC 1 cut(s) 1507
AjnI CCWGG 4 cut(s) 1964, 1981, 3609, 3677
AjuI GAANNNNNNNTTGG 2 cut(s) 3717, 3749
AleI CACNNNNGTG 1 cut(s) 1223
AloI GAACNNNNNNTCC 2 cut(s) 4307, 4339
Alw21I GWGCWC 3 cut(s) 534, 2014, 2143
Alw26I GTCTC 4 cut(s) 90, 2256, 2929, 4181
Alw44I GTGCAC 1 cut(s) 2010
AlwI GGATC 8 cut(s) 183, 863, 2076, 2760, 4323, 4421, 4568, 4716
AlwNI CAGNNNCTG 6 cut(s) 596, 794, 995, 3577, 3929, 4931
Aor13HI TCCGGA 1 cut(s) 4808
AoxI GGCC 7 cut(s) 519, 1307, 1444, 2592, 2658, 4236, 4346
ApaLI GTGCAC 1 cut(s) 2010
Asp700I GAANNNNTTC 4 cut(s) 408, 2409, 2604, 3786
AspA2I CCTAGG 1 cut(s) 1697
AspLEI GCGC 4 cut(s) 1647, 2370, 2449, 2700
AspS9I GGNCC 5 cut(s) 706, 1444, 2592, 2659, 3881
AsuC2I CCSGG 1 cut(s) 3163
AsuNHI GCTAGC 2 cut(s) 812, 4339
AvaII GGWCC 2 cut(s) 706, 3881
AvrII CCTAGG 1 cut(s) 1697
BaeGI GKGCMC 4 cut(s) 1030, 1281, 2014, 4764
BanI GGYRCC 4 cut(s) 476, 1252, 1276, 1644
BanII GRGCYC 1 cut(s) 2143
BarI GAAGNNNNNNTAC 2 cut(s) 4125, 4157
BauI CACGAG 2 cut(s) 533, 1953
BbrPI CACGTG 1 cut(s) 2582
BbsI GAAGAC 4 cut(s) 1552, 1953, 4218, 4889
Bbv12I GWGCWC 3 cut(s) 534, 2014, 2143
BbvCI CCTCAGC 1 cut(s) 3424
BceAI ACGGC 3 cut(s) 642, 3024, 4761
BcgI CGANNNNNNTGC 2 cut(s) 1065, 1099
BciT130I CCWGG 4 cut(s) 1966, 1983, 3611, 3679
BciVI GTATCC 2 cut(s) 2993, 3388
BclI TGATCA 3 cut(s) 646, 1828, 3120
BcnI CCSGG 1 cut(s) 3163
BcoDI GTCTC 4 cut(s) 90, 2256, 2929, 4181
BcuI ACTAGT 1 cut(s) 2060
BfmI CTRYAG 4 cut(s) 89, 597, 3152, 4795
BfoI RGCGCY 2 cut(s) 1648, 2701
BfrI CTTAAG 2 cut(s) 1448, 3646
BfuAI ACCTGC 2 cut(s) 236, 1501
BfuI GTATCC 2 cut(s) 2993, 3388
BglII AGATCT 1 cut(s) 3583
BlnI CCTAGG 1 cut(s) 1697
BmcAI AGTACT 1 cut(s) 748
Bme1390I CCNGG 5 cut(s) 1966, 1983, 3163, 3611, 3679
Bme18I GGWCC 2 cut(s) 706, 3881
BmeRI GACNNNNNGTC 1 cut(s) 1786
BmgBI CACGTC 1 cut(s) 1507
BmgT120I GGNCC 5 cut(s) 706, 1444, 2592, 2659, 3881
BmiI GGNNCC 9 cut(s) 250, 478, 630, 708, 1254, 1278, 1646, 4044, 4902
BmrFI CCNGG 5 cut(s) 1966, 1983, 3163, 3611, 3679
BmrI ACTGGG 1 cut(s) 3023
BmtI GCTAGC 2 cut(s) 816, 4343
BmuI ACTGGG 1 cut(s) 3023
BpiI GAAGAC 4 cut(s) 1552, 1953, 4218, 4889
BplI GAGNNNNNCTC 2 cut(s) 1181, 1213
BpmI CTGGAG 4 cut(s) 615, 828, 2735, 4299
Bpu10I CCTNAGC 4 cut(s) 2857, 3424, 3756, 3936
BpuEI CTTGAG 6 cut(s) 555, 1403, 2523, 2984, 3283, 3374
BpuMI CCSGG 1 cut(s) 3163
BsaAI YACGTR 2 cut(s) 866, 2582
BsaBI GATNNNNATC 3 cut(s) 510, 2809, 2832
BsaHI GRCGYC 2 cut(s) 895, 1645
BsaJI CCNNGG 9 cut(s) 1030, 1697, 1889, 1899, 1965, 3609, 3804, 4465, 4489
BsaWI WCCGGW 2 cut(s) 4429, 4808
BsaXI ACNNNNNCTCC 2 cut(s) 1984, 2014
Bsc4I CCNNNNNNNGG 9 cut(s) 1114, 2641, 2668, 2975, 3878, 4021, 4360, 4513, 4623
Bse1I ACTGG 8 cut(s) 632, 845, 3018, 3884, 4130, 4282, 4589, 4611
Bse8I GATNNNNATC 3 cut(s) 510, 2809, 2832
BseAI TCCGGA 1 cut(s) 4808
BseBI CCWGG 4 cut(s) 1966, 1983, 3611, 3679
BseDI CCNNGG 9 cut(s) 1030, 1697, 1889, 1899, 1965, 3609, 3804, 4465, 4489
BseJI GATNNNNATC 3 cut(s) 510, 2809, 2832
BseLI CCNNNNNNNGG 9 cut(s) 1114, 2641, 2668, 2975, 3878, 4021, 4360, 4513, 4623
BseNI ACTGG 8 cut(s) 632, 845, 3018, 3884, 4130, 4282, 4589, 4611
BseRI GAGGAG 6 cut(s) 392, 2005, 2410, 2567, 2657, 3962
BseSI GKGCMC 4 cut(s) 1030, 1281, 2014, 4764
BseYI CCCAGC 3 cut(s) 192, 2634, 4747
BsgI GTGCAG 4 cut(s) 993, 1440, 2595, 4110
Bsh1236I CGCG 1 cut(s) 2449
BshFI GGCC 7 cut(s) 521, 1309, 1446, 2594, 2660, 4238, 4348
BshNI GGYRCC 4 cut(s) 476, 1252, 1276, 1644
BsiHKAI GWGCWC 3 cut(s) 534, 2014, 2143
BsiSI CCGG 3 cut(s) 3163, 4430, 4809
BslFI GGGAC 1 cut(s) 2023
BslI CCNNNNNNNGG 9 cut(s) 1114, 2641, 2668, 2975, 3878, 4021, 4360, 4513, 4623
BsmAI GTCTC 4 cut(s) 90, 2256, 2929, 4181
BsmFI GGGAC 1 cut(s) 2023
BsmI GAATGC 3 cut(s) 127, 2229, 4341
BsnI GGCC 7 cut(s) 521, 1309, 1446, 2594, 2660, 4238, 4348
Bsp1286I GDGCHC 6 cut(s) 534, 1030, 1281, 2014, 2143, 4764
Bsp13I TCCGGA 1 cut(s) 4808
Bsp1407I TGTACA 3 cut(s) 766, 3853, 4400
Bsp19I CCATGG 2 cut(s) 1889, 1899
BspACI CCGC 7 cut(s) 561, 1350, 2470, 2521, 2876, 2911, 3574
BspANI GGCC 7 cut(s) 521, 1309, 1446, 2594, 2660, 4238, 4348
BspEI TCCGGA 1 cut(s) 4808
BspFNI CGCG 1 cut(s) 2449
BspHI TCATGA 1 cut(s) 2380
BspLI GGNNCC 9 cut(s) 250, 478, 630, 708, 1254, 1278, 1646, 4044, 4902
BspMAI CTGCAG 2 cut(s) 601, 3156
BspMI ACCTGC 2 cut(s) 236, 1501
BspOI GCTAGC 2 cut(s) 816, 4343
BspPI GGATC 8 cut(s) 183, 863, 2076, 2760, 4323, 4421, 4568, 4716
BspQI GCTCTTC 1 cut(s) 4314
BspT107I GGYRCC 4 cut(s) 476, 1252, 1276, 1644
BspTI CTTAAG 2 cut(s) 1448, 3646
BsrBI CCGCTC 1 cut(s) 2470
BsrGI TGTACA 3 cut(s) 766, 3853, 4400
BsrI ACTGG 8 cut(s) 632, 845, 3018, 3884, 4130, 4282, 4589, 4611
BssECI CCNNGG 9 cut(s) 1030, 1697, 1889, 1899, 1965, 3609, 3804, 4465, 4489
BssNAI GTATAC 2 cut(s) 1455, 3653
BssNI GRCGYC 2 cut(s) 895, 1645
BssSI CACGAG 2 cut(s) 533, 1953
BssT1I CCWWGG 7 cut(s) 1030, 1697, 1889, 1899, 3804, 4465, 4489
Bst1107I GTATAC 2 cut(s) 1455, 3653
Bst2BI CACGAG 2 cut(s) 533, 1953
Bst2UI CCWGG 4 cut(s) 1966, 1983, 3611, 3679
Bst6I CTCTTC 9 cut(s) 1182, 1913, 2069, 2279, 2526, 3744, 4059, 4314, 4887
BstACI GRCGYC 2 cut(s) 895, 1645
BstAFI CTTAAG 2 cut(s) 1448, 3646
BstAPI GCANNNNNTGC 5 cut(s) 122, 596, 2573, 3977, 4336
BstAUI TGTACA 3 cut(s) 766, 3853, 4400
BstBAI YACGTR 2 cut(s) 866, 2582
BstDSI CCRYGG 2 cut(s) 1889, 1899
BstEII GGTNACC 1 cut(s) 3621
BstFNI CGCG 1 cut(s) 2449
BstH2I RGCGCY 2 cut(s) 1648, 2701
BstHHI GCGC 4 cut(s) 1647, 2370, 2449, 2700
BstMAI GTCTC 4 cut(s) 90, 2256, 2929, 4181
BstNI CCWGG 4 cut(s) 1966, 1983, 3611, 3679
BstNSI RCATGY 3 cut(s) 1316, 2099, 2231
BstPI GGTNACC 1 cut(s) 3621
BstSCI CCNGG 5 cut(s) 1964, 1981, 3161, 3609, 3677
BstSFI CTRYAG 4 cut(s) 89, 597, 3152, 4795
BstSLI GKGCMC 4 cut(s) 1030, 1281, 2014, 4764
BstUI CGCG 1 cut(s) 2449
BstV2I GAAGAC 4 cut(s) 1552, 1953, 4218, 4889
BstX2I RGATCY 5 cut(s) 188, 2068, 3583, 4315, 4721
BstYI RGATCY 5 cut(s) 188, 2068, 3583, 4315, 4721
BstZ17I GTATAC 2 cut(s) 1455, 3653
BsuI GTATCC 2 cut(s) 2993, 3388
BsuRI GGCC 7 cut(s) 521, 1309, 1446, 2594, 2660, 4238, 4348
BtgI CCRYGG 2 cut(s) 1889, 1899
BtgZI GCGATG 1 cut(s) 1164
BtrI CACGTC 1 cut(s) 1507
BtsI GCAGTG 1 cut(s) 2251
BveI ACCTGC 2 cut(s) 236, 1501
CaiI CAGNNNCTG 6 cut(s) 596, 794, 995, 3577, 3929, 4931
CciI TCATGA 1 cut(s) 2380
CfoI GCGC 4 cut(s) 1647, 2370, 2449, 2700
Cfr13I GGNCC 5 cut(s) 706, 1444, 2592, 2659, 3881
CseI GACGC 3 cut(s) 1439, 2492, 2762
Csp6I GTAC 8 cut(s) 747, 767, 863, 1634, 3854, 3869, 4401, 4665
CviQI GTAC 8 cut(s) 747, 767, 863, 1634, 3854, 3869, 4401, 4665
DinI GGCGCC 1 cut(s) 1646
DraI TTTAAA 3 cut(s) 753, 2784, 3255
DraIII CACNNNGTG 1 cut(s) 4078
DrdI GACNNNNNNGTC 1 cut(s) 1831
DriI GACNNNNNGTC 1 cut(s) 1786
DseDI GACNNNNNNGTC 1 cut(s) 1831
Eam1104I CTCTTC 9 cut(s) 1182, 1913, 2069, 2279, 2526, 3744, 4059, 4314, 4887
Eam1105I GACNNNNNGTC 1 cut(s) 1786
EarI CTCTTC 9 cut(s) 1182, 1913, 2069, 2279, 2526, 3744, 4059, 4314, 4887
Ecl136II GAGCTC 1 cut(s) 2141
Eco130I CCWWGG 7 cut(s) 1030, 1697, 1889, 1899, 3804, 4465, 4489
Eco147I AGGCCT 1 cut(s) 4238
Eco24I GRGCYC 1 cut(s) 2143
Eco32I GATATC 1 cut(s) 4036
Eco47I GGWCC 2 cut(s) 706, 3881
Eco53kI GAGCTC 1 cut(s) 2141
Eco57I CTGAAG 6 cut(s) 423, 2093, 3246, 4307, 4340, 4552
Eco72I CACGTG 1 cut(s) 2582
Eco91I GGTNACC 1 cut(s) 3621
EcoICRI GAGCTC 1 cut(s) 2141
EcoO65I GGTNACC 1 cut(s) 3621
EcoRI GAATTC 1 cut(s) 1657
EcoRII CCWGG 4 cut(s) 1964, 1981, 3609, 3677
EcoRV GATATC 1 cut(s) 4036
EcoT14I CCWWGG 7 cut(s) 1030, 1697, 1889, 1899, 3804, 4465, 4489
EcoT22I ATGCAT 2 cut(s) 1684, 2229
EcoT38I GRGCYC 1 cut(s) 2143
EgeI GGCGCC 1 cut(s) 1646
EheI GGCGCC 1 cut(s) 1646
ErhI CCWWGG 7 cut(s) 1030, 1697, 1889, 1899, 3804, 4465, 4489
FalI AAGNNNNNCTT 6 cut(s) 570, 602, 2854, 2886, 4491, 4523
FaqI GGGAC 1 cut(s) 2023
FbaI TGATCA 3 cut(s) 646, 1828, 3120
FblI GTMKAC 2 cut(s) 1454, 3652
FriOI GRGCYC 1 cut(s) 2143
GlaI GCGC 4 cut(s) 1646, 2369, 2448, 2699
GsaI CCCAGC 3 cut(s) 196, 2638, 4751
GsuI CTGGAG 4 cut(s) 615, 828, 2735, 4299
HaeII RGCGCY 2 cut(s) 1648, 2701
HaeIII GGCC 7 cut(s) 521, 1309, 1446, 2594, 2660, 4238, 4348
HapII CCGG 3 cut(s) 3163, 4430, 4809
HgaI GACGC 3 cut(s) 1439, 2492, 2762
HhaI GCGC 4 cut(s) 1647, 2370, 2449, 2700
Hin1I GRCGYC 2 cut(s) 895, 1645
Hin6I GCGC 4 cut(s) 1645, 2368, 2447, 2698
HinP1I GCGC 4 cut(s) 1645, 2368, 2447, 2698
HincII GTYRAC 4 cut(s) 1791, 1824, 3403, 4191
HindII GTYRAC 4 cut(s) 1791, 1824, 3403, 4191
HindIII AAGCTT 1 cut(s) 2147
HpaI GTTAAC 1 cut(s) 3403
HpaII CCGG 3 cut(s) 3163, 4430, 4809
Hpy99I CGWCG 2 cut(s) 2778, 5021
HpyCH4IV ACGT 8 cut(s) 865, 895, 1506, 2560, 2581, 2793, 3655, 4704
HpySE526I ACGT 8 cut(s) 865, 895, 1506, 2560, 2581, 2793, 3655, 4704
Hsp92I GRCGYC 2 cut(s) 895, 1645
HspAI GCGC 4 cut(s) 1645, 2368, 2447, 2698
KasI GGCGCC 1 cut(s) 1644
Kpn2I TCCGGA 1 cut(s) 4808
Ksp22I TGATCA 3 cut(s) 646, 1828, 3120
KspAI GTTAAC 1 cut(s) 3403
LguI GCTCTTC 1 cut(s) 4314
MaeII ACGT 8 cut(s) 865, 895, 1506, 2560, 2581, 2793, 3655, 4704
MbiI CCGCTC 1 cut(s) 2470
MflI RGATCY 5 cut(s) 188, 2068, 3583, 4315, 4721
MhlI GDGCHC 6 cut(s) 534, 1030, 1281, 2014, 2143, 4764
Mly113I GGCGCC 1 cut(s) 1645
MlyI GAGTC 4 cut(s) 2258, 3057, 3929, 4531
MmeI TCCRAC 4 cut(s) 3091, 3522, 3818, 4999
Mph1103I ATGCAT 2 cut(s) 1684, 2229
MroI TCCGGA 1 cut(s) 4808
MroXI GAANNNNTTC 4 cut(s) 408, 2409, 2604, 3786
MslI CAYNNNNRTG 4 cut(s) 1223, 2100, 3335, 4101
MspA1I CMGCKG 5 cut(s) 2521, 3113, 3428, 3574, 4151
MspCI CTTAAG 2 cut(s) 1448, 3646
MspI CCGG 3 cut(s) 3163, 4430, 4809
MspR9I CCNGG 5 cut(s) 1966, 1983, 3163, 3611, 3679
Mva1269I GAATGC 3 cut(s) 127, 2229, 4341
MvaI CCWGG 4 cut(s) 1966, 1983, 3611, 3679
MvnI CGCG 1 cut(s) 2449
NarI GGCGCC 1 cut(s) 1645
NciI CCSGG 1 cut(s) 3163
NcoI CCATGG 2 cut(s) 1889, 1899
NheI GCTAGC 2 cut(s) 812, 4339
NlaIV GGNNCC 9 cut(s) 250, 478, 630, 708, 1254, 1278, 1646, 4044, 4902
NsiI ATGCAT 2 cut(s) 1684, 2229
NspI RCATGY 3 cut(s) 1316, 2099, 2231
OliI CACNNNNGTG 1 cut(s) 1223
PaeI GCATGC 2 cut(s) 2099, 2231
PagI TCATGA 1 cut(s) 2380
PaqCI CACCTGC 1 cut(s) 1501
PceI AGGCCT 1 cut(s) 4238
PciSI GCTCTTC 1 cut(s) 4314
PctI GAATGC 3 cut(s) 127, 2229, 4341
PdmI GAANNNNTTC 4 cut(s) 408, 2409, 2604, 3786
PflMI CCANNNNNTGG 3 cut(s) 2668, 3878, 4021
PleI GAGTC 4 cut(s) 2257, 3057, 3928, 4530
PluTI GGCGCC 1 cut(s) 1648
PmaCI CACGTG 1 cut(s) 2582
PmlI CACGTG 1 cut(s) 2582
PpsI GAGTC 4 cut(s) 2257, 3057, 3928, 4530
Ppu21I YACGTR 2 cut(s) 866, 2582
Psp124BI GAGCTC 1 cut(s) 2143
Psp6I CCWGG 4 cut(s) 1964, 1981, 3609, 3677
PspCI CACGTG 1 cut(s) 2582
PspEI GGTNACC 1 cut(s) 3621
PspFI CCCAGC 3 cut(s) 192, 2634, 4747
PspGI CCWGG 4 cut(s) 1964, 1981, 3609, 3677
PspN4I GGNNCC 9 cut(s) 250, 478, 630, 708, 1254, 1278, 1646, 4044, 4902
PspPI GGNCC 5 cut(s) 706, 1444, 2592, 2659, 3881
PstI CTGCAG 2 cut(s) 601, 3156
PstNI CAGNNNCTG 6 cut(s) 596, 794, 995, 3577, 3929, 4931
PsuI RGATCY 5 cut(s) 188, 2068, 3583, 4315, 4721
PvuII CAGCTG 3 cut(s) 3113, 3428, 4151
RsaI GTAC 8 cut(s) 748, 768, 864, 1635, 3855, 3870, 4402, 4666
RsaNI GTAC 8 cut(s) 747, 767, 863, 1634, 3854, 3869, 4401, 4665
RseI CAYNNNNRTG 4 cut(s) 1223, 2100, 3335, 4101
SacI GAGCTC 1 cut(s) 2143
SapI GCTCTTC 1 cut(s) 4314
Sau96I GGNCC 5 cut(s) 706, 1444, 2592, 2659, 3881
ScaI AGTACT 1 cut(s) 748
SchI GAGTC 4 cut(s) 2258, 3057, 3929, 4531
ScrFI CCNGG 5 cut(s) 1966, 1983, 3163, 3611, 3679
SduI GDGCHC 6 cut(s) 534, 1030, 1281, 2014, 2143, 4764
SfcI CTRYAG 4 cut(s) 89, 597, 3152, 4795
SfoI GGCGCC 1 cut(s) 1646
SinI GGWCC 2 cut(s) 706, 3881
SmiMI CAYNNNNRTG 4 cut(s) 1223, 2100, 3335, 4101
SmlI CTYRAG 8 cut(s) 570, 1382, 1448, 2538, 2963, 3262, 3353, 3646
SmoI CTYRAG 8 cut(s) 570, 1382, 1448, 2538, 2963, 3262, 3353, 3646
SpeI ACTAGT 1 cut(s) 2060
SphI GCATGC 2 cut(s) 2099, 2231
SseBI AGGCCT 1 cut(s) 4238
SsiI CCGC 7 cut(s) 561, 1350, 2470, 2521, 2876, 2911, 3574
SspDI GGCGCC 1 cut(s) 1644
SspI AATATT 1 cut(s) 610
SstI GAGCTC 1 cut(s) 2143
StuI AGGCCT 1 cut(s) 4238
StyD4I CCNGG 5 cut(s) 1964, 1981, 3161, 3609, 3677
StyI CCWWGG 7 cut(s) 1030, 1697, 1889, 1899, 3804, 4465, 4489
TaiI ACGT 8 cut(s) 868, 898, 1509, 2563, 2584, 2796, 3658, 4707
TaqI TCGA 7 cut(s) 134, 1616, 2254, 2598, 2776, 2929, 3604
TatI WGTACW 5 cut(s) 746, 766, 3853, 4400, 4664
TauI GCSGC 1 cut(s) 3577
Van91I CCANNNNNTGG 3 cut(s) 2668, 3878, 4021
Vha464I CTTAAG 2 cut(s) 1448, 3646
VneI GTGCAC 1 cut(s) 2010
VpaK11BI GGWCC 2 cut(s) 706, 3881
XbaI TCTAGA 3 cut(s) 22, 433, 1685
XceI RCATGY 3 cut(s) 1316, 2099, 2231
XcmI CCANNNNNNNNNTGG 1 cut(s) 4732
XmaJI CCTAGG 1 cut(s) 1697
XmiI GTMKAC 2 cut(s) 1454, 3652
XmnI GAANNNNTTC 4 cut(s) 408, 2409, 2604, 3786
ZraI GACGTC 1 cut(s) 896
ZrmI AGTACT 1 cut(s) 748
Zsp2I ATGCAT 2 cut(s) 1684, 2229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.