Rw7G004140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006932
Physical Location & Seq
Forward (+)
3453663 .. 3461579
7917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G004140.1

Sequence Viewer

Length: 5058 bp
ATGGAGAATAGTGGAGAAGCTCTAGATGATGATGGCGCTGGATGGTTCGAAGTGAAAAAGAAACAGAGAAGTAGCTCAAAGTTGTCTCTACAGAGTTGGGTTGGGGGAAGTTCTGGGAAGAATGCTAACTCTTCGAGTAGTCACTCTTTATCAATTGAGAACAGTAGGAATTATTTTGGCAAGCGCAGATCCCAGCTTCCAAAAGTAGGGGGAAATAGTGCTGTACATAGCCAAGGAAGTGGTGCAAGCACCATTCCTACGCCAAATAAGAGTAAAATGGGTGTTCCCTGTGATATTGGCATTGACAAGCAATGTGCTAAATGTCCCATGTCATCTCCTTCATTTATTACAAATCCTGATGGTGAAACTAGAGATCTAGAGAAGATCCCTGCAAGAGATAACTCTGAAGTGGTTCATAAAATTAAGTGGGGGGATCTAGAGGATGATGGTTTGGCACTTCCTCTTACTAACGTTGTCGGCACCAGAATCAAGTTTGGTGCTATTGGAGATGAAAATCTGGTGGCCTCCAAAGAGCATGAGAATTGTCATAGTTTTGTCCCTTCTGCAAATTCACAAGAAAACAAACTGTTTGCTGCATCTGTGGATGCCAATAGTGTTTCTCACCAGACGGCGTCAGTGAATGATAAGGATCAATTGTATGAAGATAACTGCAAGGAGGTGGTTATTATATCTGCTGAAAATGTTGGGGAGCCAATTCAGAATGACAAAATGGTTGATGTGGATAACAATACTTCAAATTGCAAGGATATACATACCGAAAACATTGAAGCAGTCGCTGATGATCCTGCAAGTGCTAGCACTTTGGCTTGTGAAGAGGGTGGGACAGTGGGGAAAGTTGAAGCACCTGTGGTTGTAACTGAGGTAAGTAATCCTGCAATTTTTGAAGAATCAGGCCCTGATGGCAGTTCAAGTGAAGTTAATATCTCCAAAGATAAAGACTTAGTTCCACCTGACAAGAGCAACCCAGAAATTTGTGCAGAGCCTACCCTCACACCATCTGGACATTATATGTTGAACTCAAATATGAGTGCCCTTGGAGATTGTGACACAGGTGAAAGCAAAGAAAGGTTTAGGCAGCGACTTTGGTGCTTTCTCTTTGAGAATCTTAACCGGGCTGTGGATGAGCTTTATCTTCTTTGTGAACTAGAGTGCGATGTAGAGCAGATGAAAGAGGCCATTCTTGTTCTTGAAGAGGCTCGCTCTGATTTCAGAGATCTGAATACTAGAGTGGAAGATTTTGAGAAAATAAAAAGGGCACCATCTCAGTTGATTGATGGGGTGCCCATCACCTTGAAGAGTGACCATCGTAGGCCACATGCTCTTTCATGGGAGGTTAGAAGAATGACAACTTCCGCACACAAAGCAGAGATACTATCCTCATCTCTTGAGGCTTTTAAGAAAATTCAGAAAGAAAGAGCTAGCTTTTGTGCAGCCAATGATGCTAAACTTATGGGCCTTAAGTACACAAATATCCAATCTAGTGATAACCTCAATATGTTACCTGCAAGAAATGATGGGAAATTTAATTCTAAAGAATCAACAATGAAGTTAAGAAGATATAGTGGAGGTTCAGATCTCGTTGAAGCTGTCACGAATGGGAATAAGAATACTGAATCAAGCAGTTTAAGCAGAGTAAAGTTGGTACAAAATGGGCGTGTCTCTCAGAATTCATCTTCCTTTGTTGTTAATGCATCTAGATTGCCTCCTAGGGACAATTCTGCTGCTGGAAAAACTAAGAGAGAACAGTCTGGGTCTGAAGCCGAGAAGATGCTTGCTAGGAAAGAAAAACTGTCAACAGAAGGTGGTGTTGAAAAAATTGCCAAGTTGACCAATCAGTCCAAAAAGCAAATTCCACTTTTGGAAAAAGATAGAGAAAAGAGGAATTCAGCTCCATGGAAATCCATGGATGCTTGGAAGGAGAAGAGGAATTGGGAGGATGTTCTTTCATCTCCTTCTCGTGTCTCTTCCCGAGTTTCACATTCGCCTGGCTTGAGGAGGAAAAGTGCTGATCGTGCACGAATGTTGCATGATAAACTAATGTCTCCTGAGAAGAAGAAAAAAACTAGTTTGGATCTGAAGAGAGAGGCAGAAGAAAAGCATGCCCGTGCTATGAGAATCAGAAGCGAGCTAGAGAATGAGAGAGCTCAGAAGCTTCATCGTAGTTCAGAAAAGATGAATAGAGTTAATGAATTACAGGCTGTAAAAAATATGAAGTTACGGGAGGGAATGCATGCTCGTCATCAACGCAGTGAGTCTCGACACGAAGCACATCTAGCTCAACGAGTGAAGAGAGCTGGTGATGAAAGCATCAAAGTTAAAGAGGTTCAGTTCATAACTTCCTTAAATGAAGAAAATAAAAAGCTCAGTTTGCGCCAGAAGCATCATGATTCAGAACTGAGGAGAGCTGAAAAACTTCAAGTGATAAGAACTAAACAAAAAGAGGATATGGCGCGAGAAGAAGCTGTTTTAGAGCGGAGGAAACTCATTGAAGCTGAAAAGTTGCAGCGTCTTGCTGAAACACAGCGGAGAAAGGAAGAGGCTCAAGTTAGAAGGGAGGAGGAGCGTAAAGCATCAAGTGCAGCACGTGAAGCAAGGGCCATCGAACAACTTCGGAGGAAGGAGGAAAGAGCCAAAGCCCAGCAGGAGGAGGCTGAACTGCTGGCCCAGAAATTGGCTGAGAGACTAAGAGAAAGTGAGCAACGCCGCAAATTTTACCTGGAACAGATACGAGAGAGAGCTTCTATGGATTTCAGGGATCAATCTTCACCTTTATTACGTCGAACTTTAAACAAGGATGTTCAGGGAAGATCATCGTCAATTAACAATGGCGATGATTATCAAGTAAGCAGTTTCTCAGGCTTAGGAAGTTGCACTTTTGCGGAAAGCAATAATACAGCACAACATTCAATGAAGCGGAGAATTAAAAAAATTCGACAGAGACTTATGGCTCTAAAATATGAAATTCTTGAGCCTCCTGTTGGTGCTGAAAATGCTGGCATTGGATACAGAACAGCACTGGGAACTGCAAGGGCAAAGATTGGGAGATGGCTTCAAGAACTTCAAAGACTCCGACAAGCAAGAAAAGAAGGGGCTGCAAGCATTGGGTTAATAACTGCTGAAATGATCAAGTATTTGGAGGGAAAGGAGCTGGAACTGCAGGCTTCCCGCCAAGCTGGTCTGATTGATTTTATCGCTTCTGCCCTGCCTGCTTCTTATACATCAAAACCTGAAGCCTGCCAGGTGACAATACATCTTTTAAAACTCTTAAGGGTGGTGTTATCTCTGCCTACAAACAGGAGTTATTTTCTTGCACAGAATCTCTTGCCCCCTATCATCCCAATGCTGTCTGCTGCCCTTGAGAGCTACATAAAGATAGCAGTATCCTTGAATCCTTCTGGTAATGTTAATTTCCCAGCTACCAAAACCTCAGTTGAAAATTTTGAATCAATATCTGAGGTGTTGGATGGTTATTTATGGACTGTAACAACAATAGTCAGTTACATAAGTTCTGATGAACGACAGCTCCAAATGAGGGATAGTTTGTTGGAGCTACTGATTGCCTACCAAGTTATTCACCGGCTGCGAGATCTCTTTGCCCTTTATGATCGACCCCAGGTGGAGGGGTCACCATTTCCATCGTCAATTATCTTAAGTATAAATTTGTTGGTTGTCTTAACTTCCAGGTCTGAAACTGATTGTCCTATTGATTGGAAAGAAGTACCTATAGAAACACTGCTTGGAAATGGAAGTGAAGATGCTAAGATTGCTGAGTGTGATAATTCGGAATGTCTTCCTTCAACTCAGACCTTGGAAGATTTTAGACCTCCATCATCATTGGTGAATGGTGGTAAATTTGTACATCTCCCAGATGTACCAGAGGATGGACCAGTAGATGAGATGTATAAGATAAATGAATCTGTGGAGTCAGTAGCTGCTGCTAAGAATAGTGAGAAAGAGCAGTCTAATAGTCTGGTTGAAGCAAACAATGCTAACACCTTCAAAACAGGTGTTCCAGATGAACCCCAGAAGATGCTGAATGAGGATATTATGAAGCCTTTTGCATCAGTGGAGGAAGAGAAACACTTGGTGGATACTGGTGCGGAACATAAGTATGTTAACAGTATGAGCTTGCAGCAACCGGTAGCATTTCTTCTTTCAGCTGTATCAGAGACAGGGCTTGTCAGTCTCCCATCTCTGTTGACATCTGTGTTATTACAGGCAAACAATAGATTGTCTTCTGAACAGGCCTCGGATTCCCTCCCATCTAATTTTGAAGATGTAGCAACTGGAGTACTGAAGGTGTTGAACAATTTGGCTCTTTTGGACCTTAAATTCATGCAGAGAATGCTAGCTAGGCCTGACCTAAAAATGGAATTTTTCCATTTGATGAGTTTCCTGCTTTCTCATTGTGCCAGCAAGTGGAAAGTAGCTAATGATCAGGTGGGGCTGCTTTTGCTTGAATCTCTTTTGCTTCTTGGTCATTTTGCCCTGTTCCACCTTGGGAATCAAGCTGTCCTTCGTTGGGGAAAGAGTCCTACCATTATCCACAAGGTATGCGATTTGCCATTTGTATTCTTCAGTGATCCTGAGTTGATGCCAGTCTTGGCTGGCACGCTGGTTGCTGCCTGTTATGGGTGTGAGCAGAACAAGGGTGTGGTTCAGCAGGAAATGAGTACAGATATGCTACTCTCCCTGTTACAATCTTGCAGAAACGTCCTGCCAGCAGTTAGATCCAATTCTAAGTTGGACAGTGGCCCAGCAGATGATGTGCCCCTAAGATCCGGCCGTAACAGTACCAAGAACTCTAGGGTTTCTTCCGGAAAGGGTGTTGCTTCAGGAAACAGCATGAGAATTGGCAAGATGAGAAGCCATAGGGAAAGTAAAACAATTAGGAGTTATGAAGAGTTGGCTCCGAAGCAGATTCTTCCATCTACAGACACTGCCTCTATGATGCTGCATTGTAGATTTCCGAACAGCTTCATCGATAGAGCAGAAAATTTCTTTTCAACTGAGAACCCCAGCATCGCTGGAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAPER_N

Protein Analysis

1685

Amino Acids

187.75

Weight (kDa)

7.86

Isoelectric Point (pI)

55.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAPER_N PF16501 359 - 459 1.1e-15 S phase cyclin A-associated protein in the endoplasmic reticulum
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1855
Acc36I ACCTGC 1 cut(s) 1531
AccB1I GGYRCC 3 cut(s) 479, 1276, 1300
AccB7I CCANNNNNTGG 3 cut(s) 2692, 3902, 4440
AccBSI CCGCTC 1 cut(s) 2494
AccII CGCG 1 cut(s) 2473
AccIII TCCGGA 1 cut(s) 4838
AciI CCGC 8 cut(s) 1374, 2494, 2545, 2725, 2900, 2935, 3187, 4121
AclI AACGTT 1 cut(s) 471
AcoI YGGCCR 1 cut(s) 4804
AcuI CTGAAG 7 cut(s) 426, 1797, 2117, 3270, 4337, 4582, 4839
AcvI CACGTG 1 cut(s) 2606
AcyI GRCGYC 1 cut(s) 632
AdeI CACNNNGTG 1 cut(s) 4108
AfaI GTAC 9 cut(s) 225, 1484, 1665, 3741, 3879, 3894, 4314, 4696, 4816
AflII CTTAAG 3 cut(s) 1478, 3286, 3670
AgeI ACCGGT 1 cut(s) 4159
AhlI ACTAGT 1 cut(s) 2084
AjnI CCWGG 5 cut(s) 2005, 2736, 3258, 3633, 3701
AjuI GAANNNNNNNTTGG 2 cut(s) 3741, 3773
AloI GAACNNNNNNTCC 2 cut(s) 3528, 3560
Alw21I GWGCWC 2 cut(s) 2038, 2167
Alw26I GTCTC 9 cut(s) 90, 1684, 1987, 2067, 2280, 2695, 2953, 4184, 4211
Alw44I GTGCAC 1 cut(s) 2034
AlwNI CAGNNNCTG 4 cut(s) 797, 917, 3953, 4961
Ama87I CYCGRG 1 cut(s) 1989
Aor13HI TCCGGA 1 cut(s) 4838
ApaLI GTGCAC 1 cut(s) 2034
AsiGI ACCGGT 1 cut(s) 4159
Asp700I GAANNNNTTC 5 cut(s) 411, 2433, 2628, 3810, 4997
AspA2I CCTAGG 1 cut(s) 1727
AspLEI GCGC 4 cut(s) 38, 186, 2394, 2473
AspS9I GGNCC 7 cut(s) 914, 1474, 2616, 2683, 3905, 4345, 4775
AsuC2I CCSGG 1 cut(s) 1133
AsuII TTCGAA 1 cut(s) 48
AsuNHI GCTAGC 3 cut(s) 815, 1439, 4369
AvaI CYCGRG 1 cut(s) 1989
AvaII GGWCC 2 cut(s) 3905, 4345
AvrII CCTAGG 1 cut(s) 1727
BaeGI GKGCMC 5 cut(s) 1054, 1279, 1305, 2038, 4794
BanI GGYRCC 3 cut(s) 479, 1276, 1300
BanII GRGCYC 1 cut(s) 2167
BarI GAAGNNNNNNTAC 2 cut(s) 4155, 4187
BauI CACGAG 1 cut(s) 1977
BbrPI CACGTG 1 cut(s) 2606
BbsI GAAGAC 2 cut(s) 3803, 4248
Bbv12I GWGCWC 2 cut(s) 2038, 2167
BceAI ACGGC 2 cut(s) 645, 4791
BcgI CGANNNNNNTGC 2 cut(s) 1089, 1123
BciT130I CCWGG 5 cut(s) 2007, 2738, 3260, 3635, 3703
BciVI GTATCC 3 cut(s) 3017, 3412, 4105
BclI TGATCA 2 cut(s) 3144, 4456
BcnI CCSGG 1 cut(s) 1133
BcoDI GTCTC 9 cut(s) 90, 1684, 1987, 2067, 2280, 2695, 2953, 4184, 4211
BcuI ACTAGT 1 cut(s) 2084
BfmI CTRYAG 4 cut(s) 89, 3176, 3744, 4953
BfoI RGCGCY 1 cut(s) 39
BfrI CTTAAG 3 cut(s) 1478, 3286, 3670
BfuAI ACCTGC 1 cut(s) 1531
BfuI GTATCC 3 cut(s) 3017, 3412, 4105
BglI GCCNNNNNGGC 1 cut(s) 921
BglII AGATCT 4 cut(s) 373, 1234, 1594, 3607
BlnI CCTAGG 1 cut(s) 1727
BmcAI AGTACT 1 cut(s) 4314
Bme1390I CCNGG 6 cut(s) 1133, 2007, 2738, 3260, 3635, 3703
Bme18I GGWCC 2 cut(s) 3905, 4345
BmeT110I CYCGRG 1 cut(s) 1989
BmgT120I GGNCC 7 cut(s) 914, 1474, 2616, 2683, 3905, 4345, 4775
BmiI GGNNCC 5 cut(s) 481, 711, 1278, 1302, 4932
BmrFI CCNGG 6 cut(s) 1133, 2007, 2738, 3260, 3635, 3703
BmrI ACTGGG 1 cut(s) 3047
BmtI GCTAGC 3 cut(s) 819, 1443, 4373
BmuI ACTGGG 1 cut(s) 3047
BpiI GAAGAC 2 cut(s) 3803, 4248
BplI GAGNNNNNCTC 2 cut(s) 1205, 1237
BpmI CTGGAG 1 cut(s) 4329
Bpu10I CCTNAGC 1 cut(s) 2881
Bpu14I TTCGAA 1 cut(s) 48
BpuEI CTTGAG 5 cut(s) 1427, 2032, 2547, 3008, 3398
BpuMI CCSGG 1 cut(s) 1133
Bsa29I ATCGAT 1 cut(s) 5004
BsaAI YACGTR 1 cut(s) 2606
BsaBI GATNNNNATC 3 cut(s) 513, 648, 2856
BsaHI GRCGYC 1 cut(s) 632
BsaJI CCNNGG 9 cut(s) 232, 1054, 1727, 1913, 1923, 3633, 3828, 4269, 4519
BsaWI WCCGGW 2 cut(s) 4159, 4838
BsaXI ACNNNNNCTCC 4 cut(s) 2008, 2038, 3528, 3558
Bse118I RCCGGY 2 cut(s) 3597, 4159
Bse1I ACTGG 5 cut(s) 3042, 3908, 4120, 4312, 4619
Bse3DI GCAATG 1 cut(s) 317
Bse8I GATNNNNATC 3 cut(s) 513, 648, 2856
BseAI TCCGGA 1 cut(s) 4838
BseBI CCWGG 5 cut(s) 2007, 2738, 3260, 3635, 3703
BseCI ATCGAT 1 cut(s) 5004
BseDI CCNNGG 9 cut(s) 232, 1054, 1727, 1913, 1923, 3633, 3828, 4269, 4519
BseJI GATNNNNATC 3 cut(s) 513, 648, 2856
BseMI GCAATG 1 cut(s) 317
BseNI ACTGG 5 cut(s) 3042, 3908, 4120, 4312, 4619
BseRI GAGGAG 5 cut(s) 2029, 2434, 2591, 2594, 2681
BseSI GKGCMC 5 cut(s) 1054, 1279, 1305, 2038, 4794
BseX3I CGGCCG 1 cut(s) 4804
BseYI CCCAGC 5 cut(s) 192, 2658, 3433, 4777, 5039
BsgI GTGCAG 3 cut(s) 1017, 1470, 2619
Bsh1236I CGCG 1 cut(s) 2473
Bsh1285I CGRYCG 1 cut(s) 4807
BshNI GGYRCC 3 cut(s) 479, 1276, 1300
BshTI ACCGGT 1 cut(s) 4159
BshVI ATCGAT 1 cut(s) 5004
BsiEI CGRYCG 1 cut(s) 4807
BsiHKAI GWGCWC 2 cut(s) 2038, 2167
BsiHKCI CYCGRG 1 cut(s) 1989
BsiSI CCGG 5 cut(s) 1132, 3598, 4160, 4803, 4839
BslFI GGGAC 4 cut(s) 309, 542, 856, 1745
BsmAI GTCTC 9 cut(s) 90, 1684, 1987, 2067, 2280, 2695, 2953, 4184, 4211
BsmFI GGGAC 4 cut(s) 309, 542, 856, 1745
BsmI GAATGC 3 cut(s) 127, 2253, 4371
BsoBI CYCGRG 1 cut(s) 1989
Bsp119I TTCGAA 1 cut(s) 48
Bsp1286I GDGCHC 6 cut(s) 1054, 1279, 1305, 2038, 2167, 4794
Bsp13I TCCGGA 1 cut(s) 4838
Bsp1407I TGTACA 2 cut(s) 223, 3877
Bsp19I CCATGG 2 cut(s) 1913, 1923
BspACI CCGC 8 cut(s) 1374, 2494, 2545, 2725, 2900, 2935, 3187, 4121
BspDI ATCGAT 1 cut(s) 5004
BspEI TCCGGA 1 cut(s) 4838
BspFNI CGCG 1 cut(s) 2473
BspHI TCATGA 1 cut(s) 2404
BspLI GGNNCC 5 cut(s) 481, 711, 1278, 1302, 4932
BspMAI CTGCAG 1 cut(s) 3180
BspMI ACCTGC 1 cut(s) 1531
BspOI GCTAGC 3 cut(s) 819, 1443, 4373
BspT104I TTCGAA 1 cut(s) 48
BspT107I GGYRCC 3 cut(s) 479, 1276, 1300
BspTI CTTAAG 3 cut(s) 1478, 3286, 3670
BsrBI CCGCTC 1 cut(s) 2494
BsrDI GCAATG 1 cut(s) 317
BsrFI RCCGGY 2 cut(s) 3597, 4159
BsrGI TGTACA 2 cut(s) 223, 3877
BsrI ACTGG 5 cut(s) 3042, 3908, 4120, 4312, 4619
BssAI RCCGGY 2 cut(s) 3597, 4159
BssECI CCNNGG 9 cut(s) 232, 1054, 1727, 1913, 1923, 3633, 3828, 4269, 4519
BssNI GRCGYC 1 cut(s) 632
BssSI CACGAG 1 cut(s) 1977
BssT1I CCWWGG 7 cut(s) 232, 1054, 1727, 1913, 1923, 3828, 4519
Bst2BI CACGAG 1 cut(s) 1977
Bst2UI CCWGG 5 cut(s) 2007, 2738, 3260, 3635, 3703
Bst4CI ACNGT 9 cut(s) 164, 588, 847, 1767, 1812, 3502, 4142, 4772, 4814
BstACI GRCGYC 1 cut(s) 632
BstAFI CTTAAG 3 cut(s) 1478, 3286, 3670
BstAPI GCANNNNNTGC 3 cut(s) 2597, 4007, 4366
BstAUI TGTACA 2 cut(s) 223, 3877
BstBAI YACGTR 1 cut(s) 2606
BstBI TTCGAA 1 cut(s) 48
BstDSI CCRYGG 2 cut(s) 1913, 1923
BstEII GGTNACC 1 cut(s) 3645
BstFNI CGCG 1 cut(s) 2473
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 4 cut(s) 38, 186, 2394, 2473
BstMAI GTCTC 9 cut(s) 90, 1684, 1987, 2067, 2280, 2695, 2953, 4184, 4211
BstMCI CGRYCG 1 cut(s) 4807
BstNI CCWGG 5 cut(s) 2007, 2738, 3260, 3635, 3703
BstNSI RCATGY 3 cut(s) 1340, 2123, 2255
BstPI GGTNACC 1 cut(s) 3645
BstSCI CCNGG 6 cut(s) 1131, 2005, 2736, 3258, 3633, 3701
BstSFI CTRYAG 4 cut(s) 89, 3176, 3744, 4953
BstSLI GKGCMC 5 cut(s) 1054, 1279, 1305, 2038, 4794
BstUI CGCG 1 cut(s) 2473
BstV2I GAAGAC 2 cut(s) 3803, 4248
BstXI CCANNNNNNTGG 1 cut(s) 239
BstZI CGGCCG 1 cut(s) 4804
Bsu15I ATCGAT 1 cut(s) 5004
BsuI GTATCC 3 cut(s) 3017, 3412, 4105
BsuTUI ATCGAT 1 cut(s) 5004
BtgI CCRYGG 2 cut(s) 1913, 1923
BtgZI GCGATG 3 cut(s) 1188, 2865, 5029
BtsI GCAGTG 3 cut(s) 2275, 3752, 4959
BtsIMutI CAGTG 9 cut(s) 642, 852, 2275, 3035, 3752, 4092, 4606, 4777, 4959
BveI ACCTGC 1 cut(s) 1531
CaiI CAGNNNCTG 4 cut(s) 797, 917, 3953, 4961
CciI TCATGA 1 cut(s) 2404
CfoI GCGC 4 cut(s) 38, 186, 2394, 2473
Cfr10I RCCGGY 2 cut(s) 3597, 4159
Cfr13I GGNCC 7 cut(s) 914, 1474, 2616, 2683, 3905, 4345, 4775
ClaI ATCGAT 1 cut(s) 5004
CseI GACGC 2 cut(s) 621, 2516
Csp6I GTAC 9 cut(s) 224, 1483, 1664, 3740, 3878, 3893, 4313, 4695, 4815
CspAI ACCGGT 1 cut(s) 4159
CviQI GTAC 9 cut(s) 224, 1483, 1664, 3740, 3878, 3893, 4313, 4695, 4815
DraI TTTAAA 2 cut(s) 2808, 3279
DraIII CACNNNGTG 1 cut(s) 4108
DrdI GACNNNNNNGTC 1 cut(s) 1855
DseDI GACNNNNNNGTC 1 cut(s) 1855
EaeI YGGCCR 1 cut(s) 4804
EagI CGGCCG 1 cut(s) 4804
Ecl136II GAGCTC 1 cut(s) 2165
EclXI CGGCCG 1 cut(s) 4804
Eco130I CCWWGG 7 cut(s) 232, 1054, 1727, 1913, 1923, 3828, 4519
Eco147I AGGCCT 2 cut(s) 4268, 4378
Eco24I GRGCYC 1 cut(s) 2167
Eco47I GGWCC 2 cut(s) 3905, 4345
Eco52I CGGCCG 1 cut(s) 4804
Eco53kI GAGCTC 1 cut(s) 2165
Eco57I CTGAAG 7 cut(s) 426, 1797, 2117, 3270, 4337, 4582, 4839
Eco72I CACGTG 1 cut(s) 2606
Eco88I CYCGRG 1 cut(s) 1989
Eco91I GGTNACC 1 cut(s) 3645
EcoICRI GAGCTC 1 cut(s) 2165
EcoO109I RGGNCCY 1 cut(s) 914
EcoO65I GGTNACC 1 cut(s) 3645
EcoRI GAATTC 2 cut(s) 1687, 1903
EcoRII CCWGG 5 cut(s) 2005, 2736, 3258, 3633, 3701
EcoT14I CCWWGG 7 cut(s) 232, 1054, 1727, 1913, 1923, 3828, 4519
EcoT22I ATGCAT 2 cut(s) 1714, 2253
EcoT38I GRGCYC 1 cut(s) 2167
ErhI CCWWGG 7 cut(s) 232, 1054, 1727, 1913, 1923, 3828, 4519
FalI AAGNNNNNCTT 4 cut(s) 2878, 2910, 4521, 4553
FaqI GGGAC 4 cut(s) 309, 542, 856, 1745
FauI CCCGC 1 cut(s) 3194
FbaI TGATCA 2 cut(s) 3144, 4456
FriOI GRGCYC 1 cut(s) 2167
GlaI GCGC 4 cut(s) 37, 185, 2393, 2472
GsaI CCCAGC 5 cut(s) 196, 2662, 3437, 4781, 5043
GsuI CTGGAG 1 cut(s) 4329
HaeII RGCGCY 1 cut(s) 39
HapII CCGG 5 cut(s) 1132, 3598, 4160, 4803, 4839
HgaI GACGC 2 cut(s) 621, 2516
HhaI GCGC 4 cut(s) 38, 186, 2394, 2473
Hin1I GRCGYC 1 cut(s) 632
Hin6I GCGC 4 cut(s) 36, 184, 2392, 2471
HinP1I GCGC 4 cut(s) 36, 184, 2392, 2471
HincII GTYRAC 4 cut(s) 1815, 1848, 4138, 4221
HindII GTYRAC 4 cut(s) 1815, 1848, 4138, 4221
HindIII AAGCTT 1 cut(s) 2171
HpaI GTTAAC 1 cut(s) 4138
HpaII CCGG 5 cut(s) 1132, 3598, 4160, 4803, 4839
Hpy166II GTNNAC 7 cut(s) 1163, 1485, 1815, 1848, 2036, 4138, 4221
Hpy8I GTNNAC 7 cut(s) 1163, 1485, 1815, 1848, 2036, 4138, 4221
Hpy99I CGWCG 1 cut(s) 2802
HpyCH4III ACNGT 9 cut(s) 164, 588, 847, 1767, 1812, 3502, 4142, 4772, 4814
HpyCH4IV ACGT 4 cut(s) 471, 2605, 2797, 4734
HpySE526I ACGT 4 cut(s) 471, 2605, 2797, 4734
Hsp92I GRCGYC 1 cut(s) 632
HspAI GCGC 4 cut(s) 36, 184, 2392, 2471
Kpn2I TCCGGA 1 cut(s) 4838
Ksp22I TGATCA 2 cut(s) 3144, 4456
KspAI GTTAAC 1 cut(s) 4138
LmnI GCTCC 7 cut(s) 709, 1915, 2581, 3166, 3549, 3568, 4936
MaeII ACGT 4 cut(s) 471, 2605, 2797, 4734
MbiI CCGCTC 1 cut(s) 2494
MfeI CAATTG 2 cut(s) 153, 653
MhlI GDGCHC 6 cut(s) 1054, 1279, 1305, 2038, 2167, 4794
MlyI GAGTC 4 cut(s) 2282, 3081, 3953, 4561
MmeI TCCRAC 4 cut(s) 3115, 3462, 3546, 4746
Mph1103I ATGCAT 2 cut(s) 1714, 2253
MroI TCCGGA 1 cut(s) 4838
MroXI GAANNNNTTC 5 cut(s) 411, 2433, 2628, 3810, 4997
MslI CAYNNNNRTG 3 cut(s) 2124, 3359, 4131
MspA1I CMGCKG 2 cut(s) 2545, 4181
MspCI CTTAAG 3 cut(s) 1478, 3286, 3670
MspI CCGG 5 cut(s) 1132, 3598, 4160, 4803, 4839
MspR9I CCNGG 6 cut(s) 1133, 2007, 2738, 3260, 3635, 3703
MunI CAATTG 2 cut(s) 153, 653
Mva1269I GAATGC 3 cut(s) 127, 2253, 4371
MvaI CCWGG 5 cut(s) 2007, 2738, 3260, 3635, 3703
MvnI CGCG 1 cut(s) 2473
NciI CCSGG 1 cut(s) 1133
NcoI CCATGG 2 cut(s) 1913, 1923
NheI GCTAGC 3 cut(s) 815, 1439, 4369
NlaIV GGNNCC 5 cut(s) 481, 711, 1278, 1302, 4932
NmeAIII GCCGAG 1 cut(s) 1807
NmuCI GTSAC 6 cut(s) 140, 1064, 1319, 1609, 3262, 3645
NsiI ATGCAT 2 cut(s) 1714, 2253
NspI RCATGY 3 cut(s) 1340, 2123, 2255
NspV TTCGAA 1 cut(s) 48
PaeI GCATGC 2 cut(s) 2123, 2255
PagI TCATGA 1 cut(s) 2404
PceI AGGCCT 2 cut(s) 4268, 4378
PctI GAATGC 3 cut(s) 127, 2253, 4371
PdmI GAANNNNTTC 5 cut(s) 411, 2433, 2628, 3810, 4997
PflFI GACNNNGTC 1 cut(s) 631
PflMI CCANNNNNTGG 3 cut(s) 2692, 3902, 4440
PinAI ACCGGT 1 cut(s) 4159
PleI GAGTC 4 cut(s) 2281, 3081, 3952, 4560
PmaCI CACGTG 1 cut(s) 2606
PmlI CACGTG 1 cut(s) 2606
PpsI GAGTC 4 cut(s) 2281, 3081, 3952, 4560
Ppu21I YACGTR 1 cut(s) 2606
Psp124BI GAGCTC 1 cut(s) 2167
Psp1406I AACGTT 1 cut(s) 471
Psp6I CCWGG 5 cut(s) 2005, 2736, 3258, 3633, 3701
PspCI CACGTG 1 cut(s) 2606
PspEI GGTNACC 1 cut(s) 3645
PspFI CCCAGC 5 cut(s) 192, 2658, 3433, 4777, 5039
PspGI CCWGG 5 cut(s) 2005, 2736, 3258, 3633, 3701
PspN4I GGNNCC 5 cut(s) 481, 711, 1278, 1302, 4932
PspPI GGNCC 7 cut(s) 914, 1474, 2616, 2683, 3905, 4345, 4775
PstI CTGCAG 1 cut(s) 3180
PstNI CAGNNNCTG 4 cut(s) 797, 917, 3953, 4961
PsyI GACNNNGTC 1 cut(s) 631
PvuII CAGCTG 1 cut(s) 4181
RsaI GTAC 9 cut(s) 225, 1484, 1665, 3741, 3879, 3894, 4314, 4696, 4816
RsaNI GTAC 9 cut(s) 224, 1483, 1664, 3740, 3878, 3893, 4313, 4695, 4815
RseI CAYNNNNRTG 3 cut(s) 2124, 3359, 4131
SacI GAGCTC 1 cut(s) 2167
Sau96I GGNCC 7 cut(s) 914, 1474, 2616, 2683, 3905, 4345, 4775
ScaI AGTACT 1 cut(s) 4314
SchI GAGTC 4 cut(s) 2282, 3081, 3953, 4561
ScrFI CCNGG 6 cut(s) 1133, 2007, 2738, 3260, 3635, 3703
SduI GDGCHC 6 cut(s) 1054, 1279, 1305, 2038, 2167, 4794
SfcI CTRYAG 4 cut(s) 89, 3176, 3744, 4953
SfuI TTCGAA 1 cut(s) 48
SinI GGWCC 2 cut(s) 3905, 4345
SmiMI CAYNNNNRTG 3 cut(s) 2124, 3359, 4131
SmlI CTYRAG 8 cut(s) 1406, 1478, 2011, 2562, 2987, 3286, 3377, 3670
SmoI CTYRAG 8 cut(s) 1406, 1478, 2011, 2562, 2987, 3286, 3377, 3670
SpeI ACTAGT 1 cut(s) 2084
SphI GCATGC 2 cut(s) 2123, 2255
SseBI AGGCCT 2 cut(s) 4268, 4378
SsiI CCGC 8 cut(s) 1374, 2494, 2545, 2725, 2900, 2935, 3187, 4121
SstI GAGCTC 1 cut(s) 2167
StuI AGGCCT 2 cut(s) 4268, 4378
StyD4I CCNGG 6 cut(s) 1131, 2005, 2736, 3258, 3633, 3701
StyI CCWWGG 7 cut(s) 232, 1054, 1727, 1913, 1923, 3828, 4519
TaaI ACNGT 9 cut(s) 164, 588, 847, 1767, 1812, 3502, 4142, 4772, 4814
TaiI ACGT 4 cut(s) 474, 2608, 2800, 4737
TaqI TCGA 8 cut(s) 48, 134, 2278, 2622, 2800, 2953, 3628, 5004
TatI WGTACW 5 cut(s) 223, 1482, 3877, 4312, 4694
TauI GCSGC 1 cut(s) 2727
TscAI CASTG 9 cut(s) 642, 852, 2275, 3042, 3759, 4092, 4606, 4777, 4966
TseFI GTSAC 6 cut(s) 140, 1064, 1319, 1609, 3262, 3645
Tsp45I GTSAC 6 cut(s) 140, 1064, 1319, 1609, 3262, 3645
TspRI CASTG 9 cut(s) 642, 852, 2275, 3042, 3759, 4092, 4606, 4777, 4966
Tth111I GACNNNGTC 1 cut(s) 631
Van91I CCANNNNNTGG 3 cut(s) 2692, 3902, 4440
Vha464I CTTAAG 3 cut(s) 1478, 3286, 3670
VneI GTGCAC 1 cut(s) 2034
VpaK11BI GGWCC 2 cut(s) 3905, 4345
XbaI TCTAGA 4 cut(s) 22, 376, 436, 1715
XceI RCATGY 3 cut(s) 1340, 2123, 2255
XcmI CCANNNNNNNNNTGG 1 cut(s) 4762
XmaJI CCTAGG 1 cut(s) 1727
XmnI GAANNNNTTC 5 cut(s) 411, 2433, 2628, 3810, 4997
ZrmI AGTACT 1 cut(s) 4314
Zsp2I ATGCAT 2 cut(s) 1714, 2253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.