MD14G1208500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006932
Physical Location & Seq
Reverse (-)
29501878 .. 29506607
4730 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1208500.v1.1.491

Sequence Viewer

Length: 3648 bp
ATGGAGAACAGTGGAGAAGCAGCAGATGATGAGGGCTCTGGATGGTTCGAAGTGAAAAAGAAAAACAGAAGTAGCTCAAAGTTCTCTCTACAGAGTTGGGTTGGGGGGTTTTCTGGAAAAAATGCTTCTAACCATTCGAGTAGTCGGACCTCTACAAGTGAAAGCAGTGGTTATTCTCGGGACAAGCGCAGATCCCAGCCTCCAAAGGCACCGGGAAATTATGGTGTATATAGCCGGGGAAGTGATGCGAATTCTATTGCTGTCCCAAATGAAAAGAAACCGGGTGCTCCCTATCACGATATCTGTGTTATTAAGCAAGATACTGGATGTCCTAAGCCACCTCCTTCCTTTGTTACAAATTCTGATGATGGAACTATAGATGCTGAGAAAATCCCTGCAAACGATAACTCTGAAGTAGTTGCTAAAATCAAGTGGGGGGATCTCGAGGGCGATGGTTTGGCACTCCCTTGTGCTAACCTTGTCGGTGCCAAAATCAAGTTTGGTGCTATCGGAGATGATAATCTGGTGGTCTCGAGTGAGCATGGAAATGGTCATAAGTTTGTTTCCTCTACAAATTTACAAAAAAACACATTGGTTGCAGAATCTGTGGATGCTAAGGACCAATTATGTGAAGATAAATGTAAAGAGGTGAATATAATCTCTTCTGAAAAGACTGAAGAGCCAATTTTGAATGAAAAAAGGCTTGATCTTGATAACAGTACTTCGAATTGCAAGGATATAGATACTGAACACGTTGAAGTAGTAGCTGATGATGGTTTAAGTGCCAGTCCTTTAGCTGGTGAAGAGGCTGCGACGGTGGAGAAATGTCAGACACCTGTTGTTCTACCTGAGGTAGGTGACCATGAAATTCCTGAAGTATCAGTGATAACTGGGGATTCAATTGAAGTTCATGTTTCCGAAGATAAAGGGTTAATTCCACCAAAGAGGGATCATGGAATTTCTGAGGTGTCTACTTACACAGCCTCCGATGAGGACCATGAGGGTACACAATGTGGTATGATACATGATATGTCAAACTCACAAAATATGAGTGCTCTTGGAGAATGTGACACAGATGAAAGCAAAGAAAGGTTTAGGCAGCGGCTTTGGTGCTTTCTTTTTGAGAATCTCAACAGGGCTGTGGATGAACTTTATCTTCTTTGCGAACTAGAATGCGATGTAGAGCAGATGAAAGAGGCAATTCTTGTTCTTGAAGAGGCTGCCTCTGATTTTCGAGATCTAAGCACTAGAGTGGAAGACTTTGAGGAAATAAAAAGGTCGTCATCCCAGATAATTGATGGTGTGCCCATTACCTTGAAGAGCGACCACCGTAGGCCGCATGCTCTCTCATGGGAGGTTAGAAGAATGACAACTTCAGCACACAAAGCTGAAATACTATCGTCATCTCTTGAGGCGTTTAAGAAAATTCAACAAGAAAGAGCTAGCTTGTGTGCAACTACTGATGATAAACTTTTGGGGCCTCAGTACCTAAATCTCCGATGTGGTGATAAGCTGAATAAGTTATCTGCCATAAATGATGGGAAATTTAATACTAAAGATTCAATAAAGAAGTCAGGAAAGCAGAATGGAGGTTCGGATCTTGCTGAAGCTGACATAAATGGAGGAAAAGGCAATACTGAGTCAAGCTGTTCGAGCAAAACAAACTTGGTACAAAGTGCACGTGCCCCGCATAATGCATCTTCCTCTGTTGTTAATGCATCTAGGCTGCCTCCTAGGGATAGTTCTGCTGCTGGAAAGACTAAGAGTAAACAGTTTGGATCCGAAGCCGAGAGGCTGCTCCCTAAGAAAGATAAATTGTCAACAGAAGGTGGTGTTGAAAAATTCCCCAGATTGACGGATCAATCCAAAAAGGAAATTCCTCTATTTGAGAAAGATAAAGAAAAGAGGAACTCAGCTCCATGGAAATCTATGGATGCTTGGAAAGAGAAGAGGAACTGGGAGGACGTACTTTCCTCTCCTTCCCGTGTCTCTTCTCGTGTTTCACGTTCACCTGGAATGAGGAGGAAAAGTGCTGACCGTGCACGTATGCTGCATTATAAACTAATGTCTCCTGAGAAGAAGAAGAAAACTGCTCTCGATCTGAAGAGAGAAGCAGAAGAAAAACATGCTCGTGCTCTGAGAATTAAAAGTGAGCTAGATAATGAGAGAGCTCAAAAACTTCATCGTAATTCAGAAAAGGTGTATCGAGCTAGTGAATTTCACGTTGTTCGCAACATGAAGTTACGTGAAGGAATATATGCTCGTCATCAACGCAGCGAGTCTCGCCACGAAGCTTTTCTGGCTCAAGTTGTAAAGAGAGCTGGTGATGAAAGCAGTAAAGTTAATGAGGTTCGTTTCATAACTTCCTTAAATGAAGAAAATAAAAAACTTAGTTTGCGCCAGAAGCTTCATGATTCAGAGTTGAGGAGAGCTGAAAAACTTCAAGTGATAAGAACTAAACAGAAAGAGGATATGGCAAGAGAAGAAGCTGTTTTAGAACGGAGGAAACTCATCGAAGCTGAAAAGTTGCAGCGTCTTGCTGAGACACAGAAAAGAAAGGAAGAGGCTCTAGTTAGAAGAGAAGAGGAACGCAAAGCATCAAGTGCAGCCCGTGAAGCAAGGGCCATGGAACAACTTCGTAGGAAGGAGGAAAGAGCCAAAGCTCAGCAGGAGGAGGCTGAACTTCTGGCCCAGAAATTGGCTGAGAGACTTAGTGAAAGTGAGCAACGCCGAAAGTTTTACCTGGAGCAGATACGAGAGAGAGCTTCTATGGATTTCAGGGATCAATCTTCGCCTTTATTCCGCCGAACTCTGAACAAGGAGGGTCAGGGAAGATCTTCATCGGTCAACAATGGTGATGAGTATCAAGGGACCAACTTCTCAGGCTTAGGAGGTTCTACTATTGTGGCAAGCAATGTTACAGCACAGCATTCAATGAAGCGGAGAATCAAAAGAATTCGGCAAAGACTTATGGCTCTAAAATATGATTTTCCCGAGCCTCCTGCTGGTGCTGAAAATGCTGGCATTGGATACAGAACGGCTGTGGGAACTGCAAGGGCAAAGATTGGAAGATGGCTTCAAGAACTTAAAACACTCCGGCAGGCAAGAAAAGAAGGGGCTGCAAGTATTGGGTTAATAATTGCTGAAATGATCAAGTATTTGGAGGGAAAGGAGCCTGAACTGCAGGCTTCCCGCCAAGCTGGTTTGCTTGATTTCATAGCTTCTACTTTACCTGCTTCTCATACATCAAAACCTGAAGCCTGCCAAGTGACAATACACCTTTTAAAGCTTTTGAGGGTAGTGTTATCAGTGCCTGCAAACAGGAGTTATTTTCTCATACAGAATCTCTTACCTCCTATCATCCCGATGCTGGCAGCAGCCCTCGAGAGCTACATAAAGATGGCAGTATCCTTGAACCTTTCGGCTAATGTTAATTCCCTATCAAGCAAAACCTCAGCTGAAAATTTTGAATCGATGTCTGAGGTTCTGGATGGTTATTTATGGACTGTGACAACAATCATCAGTCATATAAGTTCAGATGAACGACAGCTCCAAATGCGGGATGGCTTGCTTGAGCTGTTGATTGCTTACCAAGTTATTCAACGGCTGCGAGATCTCTTTGCACTTTATGATCGGCCCCAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAPER_N

Protein Analysis

1216

Amino Acids

135.8

Weight (kDa)

8.47

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAPER_N PF16501 359 - 460 2e-15 S phase cyclin A-associated protein in the endoplasmic reticulum
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2058
Acc36I ACCTGC 1 cut(s) 3241
AccB1I GGYRCC 2 cut(s) 208, 485
AccB7I CCANNNNNTGG 1 cut(s) 2698
AccI GTMKAC 1 cut(s) 971
AciI CCGC 7 cut(s) 1102, 1337, 1688, 2803, 2941, 3193, 3559
AclWI GGATC 8 cut(s) 186, 447, 957, 1605, 1773, 1786, 1866, 2790
AcuI CTGAAG 7 cut(s) 432, 696, 894, 1359, 1626, 2123, 3276
AcvI CACGTG 1 cut(s) 1682
AdeI CACNNNGTG 1 cut(s) 1013
AfaI GTAC 5 cut(s) 721, 1006, 1487, 1671, 1968
AfiI CCNNNNNNNGG 6 cut(s) 797, 854, 2698, 3005, 3370, 3559
AflIII ACRYGT 1 cut(s) 751
AjnI CCWGG 2 cut(s) 2011, 2742
AleI CACNNNNGTG 1 cut(s) 1250
AloI GAACNNNNNNTCC 2 cut(s) 3534, 3566
Alw21I GWGCWC 6 cut(s) 289, 1057, 1681, 2044, 2137, 2173
Alw26I GTCTC 6 cut(s) 535, 1993, 2073, 2286, 2537, 2701
Alw44I GTGCAC 2 cut(s) 1677, 2040
AlwI GGATC 8 cut(s) 186, 447, 957, 1605, 1773, 1786, 1866, 2790
AlwNI CAGNNNCTG 2 cut(s) 605, 3314
Ama87I CYCGRG 5 cut(s) 177, 443, 532, 2993, 3383
AoxI GGCC 5 cut(s) 1334, 1478, 2622, 2688, 3635
ApaLI GTGCAC 2 cut(s) 1677, 2040
Asp700I GAANNNNTTC 4 cut(s) 2295, 2439, 2634, 2836
AspA2I CCTAGG 1 cut(s) 1733
AspLEI GCGC 2 cut(s) 189, 2400
AspS9I GGNCC 8 cut(s) 147, 619, 994, 1478, 2622, 2689, 2871, 3636
AsuC2I CCSGG 3 cut(s) 213, 236, 282
AsuHPI GGTGA 7 cut(s) 661, 812, 869, 1517, 2001, 2336, 2867
AsuII TTCGAA 2 cut(s) 48, 725
AsuNHI GCTAGC 1 cut(s) 1442
AvaI CYCGRG 5 cut(s) 177, 443, 532, 2993, 3383
AvaII GGWCC 4 cut(s) 147, 619, 994, 2871
AvrII CCTAGG 1 cut(s) 1733
AxyI CCTNAGG 1 cut(s) 849
BaeGI GKGCMC 4 cut(s) 1308, 1681, 1687, 2044
BamHI GGATCC 1 cut(s) 1778
BanI GGYRCC 2 cut(s) 208, 485
BanII GRGCYC 2 cut(s) 38, 2173
BauI CACGAG 2 cut(s) 1995, 2130
BbrPI CACGTG 1 cut(s) 1682
BbsI GAAGAC 1 cut(s) 1263
Bbv12I GWGCWC 6 cut(s) 289, 1057, 1681, 2044, 2137, 2173
BbvCI CCTCAGC 1 cut(s) 3454
BceAI ACGGC 2 cut(s) 3054, 3620
BcgI CGANNNNNNTGC 2 cut(s) 2984, 3018
BciT130I CCWGG 2 cut(s) 2013, 2744
BciVI GTATCC 2 cut(s) 3023, 3418
BclI TGATCA 1 cut(s) 3150
BcnI CCSGG 3 cut(s) 213, 236, 282
BcoDI GTCTC 6 cut(s) 535, 1993, 2073, 2286, 2537, 2701
BfaI CTAG 8 cut(s) 1169, 1248, 1443, 1722, 1734, 2156, 2211, 2570
BfmI CTRYAG 3 cut(s) 89, 375, 3182
BfuAI ACCTGC 1 cut(s) 3241
BfuI GTATCC 2 cut(s) 3023, 3418
BglII AGATCT 4 cut(s) 1237, 2834, 3613, 3642
BlnI CCTAGG 1 cut(s) 1733
BlpI GCTNAGC 1 cut(s) 2664
BmcAI AGTACT 1 cut(s) 721
Bme1390I CCNGG 5 cut(s) 213, 236, 282, 2013, 2744
Bme18I GGWCC 4 cut(s) 147, 619, 994, 2871
BmeT110I CYCGRG 5 cut(s) 177, 443, 532, 2993, 3383
BmgT120I GGNCC 8 cut(s) 147, 619, 994, 1478, 2622, 2689, 2871, 3636
BmiI GGNNCC 7 cut(s) 210, 487, 1479, 1780, 2872, 3174, 3638
BmrFI CCNGG 5 cut(s) 213, 236, 282, 2013, 2744
BmrI ACTGGG 2 cut(s) 900, 1966
BmsI GCATC 8 cut(s) 235, 370, 601, 1706, 1727, 1924, 2606, 3357
BmtI GCTAGC 1 cut(s) 1446
BmuI ACTGGG 2 cut(s) 900, 1966
BpiI GAAGAC 1 cut(s) 1263
BplI GAGNNNNNCTC 2 cut(s) 1208, 1240
BpmI CTGGAG 1 cut(s) 2765
Bpu10I CCTNAGC 4 cut(s) 333, 615, 2887, 3454
Bpu1102I GCTNAGC 1 cut(s) 2664
Bpu14I TTCGAA 2 cut(s) 48, 725
BpuEI CTTGAG 3 cut(s) 1430, 2289, 3593
BpuMI CCSGG 3 cut(s) 213, 236, 282
Bsa29I ATCGAT 1 cut(s) 3473
BsaAI YACGTR 3 cut(s) 1682, 2045, 2246
BsaBI GATNNNNATC 3 cut(s) 519, 2839, 2862
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 4 cut(s) 235, 1733, 1919, 2625
BsaXI ACNNNNNCTCC 6 cut(s) 968, 998, 2014, 2044, 3534, 3564
Bsc4I CCNNNNNNNGG 6 cut(s) 797, 854, 2698, 3005, 3370, 3559
Bse1I ACTGG 4 cut(s) 328, 786, 895, 1961
Bse21I CCTNAGG 1 cut(s) 849
Bse3DI GCAATG 1 cut(s) 2920
Bse8I GATNNNNATC 3 cut(s) 519, 2839, 2862
BseBI CCWGG 2 cut(s) 2013, 2744
BseCI ATCGAT 1 cut(s) 3473
BseDI CCNNGG 4 cut(s) 235, 1733, 1919, 2625
BseGI GGATG 9 cut(s) 47, 332, 616, 1150, 1283, 1939, 3360, 3496, 3568
BseJI GATNNNNATC 3 cut(s) 519, 2839, 2862
BseLI CCNNNNNNNGG 6 cut(s) 797, 854, 2698, 3005, 3370, 3559
BseMI GCAATG 1 cut(s) 2920
BseNI ACTGG 4 cut(s) 328, 786, 895, 1961
BseRI GAGGAG 3 cut(s) 2035, 2440, 2687
BseSI GKGCMC 4 cut(s) 1308, 1681, 1687, 2044
BseYI CCCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 2625
BshFI GGCC 5 cut(s) 1336, 1480, 2624, 2690, 3637
BshNI GGYRCC 2 cut(s) 208, 485
BshVI ATCGAT 1 cut(s) 3473
BsiHKAI GWGCWC 6 cut(s) 289, 1057, 1681, 2044, 2137, 2173
BsiHKCI CYCGRG 5 cut(s) 177, 443, 532, 2993, 3383
BsiSI CCGG 4 cut(s) 212, 235, 281, 3097
BslFI GGGAC 3 cut(s) 194, 248, 2884
BslI CCNNNNNNNGG 6 cut(s) 797, 854, 2698, 3005, 3370, 3559
BsmAI GTCTC 6 cut(s) 535, 1993, 2073, 2286, 2537, 2701
BsmFI GGGAC 3 cut(s) 194, 248, 2884
BsmI GAATGC 2 cut(s) 1178, 2929
BsnI GGCC 5 cut(s) 1336, 1480, 2624, 2690, 3637
Bso31I GGTCTC 1 cut(s) 535
BsoBI CYCGRG 5 cut(s) 177, 443, 532, 2993, 3383
Bsp119I TTCGAA 2 cut(s) 48, 725
Bsp1286I GDGCHC 9 cut(s) 38, 289, 1057, 1308, 1681, 1687, 2044, 2137, 2173
Bsp1720I GCTNAGC 1 cut(s) 2664
Bsp19I CCATGG 2 cut(s) 1919, 2625
BspACI CCGC 7 cut(s) 1102, 1337, 1688, 2803, 2941, 3193, 3559
BspANI GGCC 5 cut(s) 1336, 1480, 2624, 2690, 3637
BspDI ATCGAT 1 cut(s) 3473
BspHI TCATGA 1 cut(s) 2410
BspLI GGNNCC 7 cut(s) 210, 487, 1479, 1780, 2872, 3174, 3638
BspMAI CTGCAG 1 cut(s) 3186
BspMI ACCTGC 1 cut(s) 3241
BspOI GCTAGC 1 cut(s) 1446
BspPI GGATC 8 cut(s) 186, 447, 957, 1605, 1773, 1786, 1866, 2790
BspQI GCTCTTC 2 cut(s) 672, 1313
BspT104I TTCGAA 2 cut(s) 48, 725
BspT107I GGYRCC 2 cut(s) 208, 485
BspTNI GGTCTC 1 cut(s) 535
BsrDI GCAATG 1 cut(s) 2920
BsrI ACTGG 4 cut(s) 328, 786, 895, 1961
BssECI CCNNGG 4 cut(s) 235, 1733, 1919, 2625
BssSI CACGAG 2 cut(s) 1995, 2130
BssT1I CCWWGG 3 cut(s) 1733, 1919, 2625
Bst2BI CACGAG 2 cut(s) 1995, 2130
Bst2UI CCWGG 2 cut(s) 2013, 2744
Bst4CI ACNGT 7 cut(s) 11, 719, 817, 1331, 1773, 2039, 3508
BstAPI GCANNNNNTGC 1 cut(s) 2603
BstBAI YACGTR 3 cut(s) 1682, 2045, 2246
BstBI TTCGAA 2 cut(s) 48, 725
BstDSI CCRYGG 2 cut(s) 1919, 2625
BstEII GGTNACC 1 cut(s) 857
BstENI CCTNNNNNAGG 1 cut(s) 852
BstF5I GGATG 9 cut(s) 47, 332, 616, 1150, 1283, 1939, 3360, 3496, 3568
BstHHI GCGC 2 cut(s) 189, 2400
BstMAI GTCTC 6 cut(s) 535, 1993, 2073, 2286, 2537, 2701
BstNI CCWGG 2 cut(s) 2013, 2744
BstNSI RCATGY 2 cut(s) 1343, 2129
BstPI GGTNACC 1 cut(s) 857
BstSCI CCNGG 5 cut(s) 211, 234, 280, 2011, 2742
BstSFI CTRYAG 3 cut(s) 89, 375, 3182
BstSLI GKGCMC 4 cut(s) 1308, 1681, 1687, 2044
BstV2I GAAGAC 1 cut(s) 1263
BstX2I RGATCY 8 cut(s) 191, 439, 1237, 1597, 1778, 2834, 3613, 3642
BstYI RGATCY 8 cut(s) 191, 439, 1237, 1597, 1778, 2834, 3613, 3642
Bsu15I ATCGAT 1 cut(s) 3473
Bsu36I CCTNAGG 1 cut(s) 849
BsuI GTATCC 2 cut(s) 3023, 3418
BsuRI GGCC 5 cut(s) 1336, 1480, 2624, 2690, 3637
BsuTUI ATCGAT 1 cut(s) 3473
BtgI CCRYGG 2 cut(s) 1919, 2625
BtgZI GCGATG 2 cut(s) 465, 1191
BtsCI GGATG 9 cut(s) 47, 332, 616, 1150, 1283, 1939, 3360, 3496, 3568
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 4 cut(s) 16, 172, 888, 3315
BveI ACCTGC 1 cut(s) 3241
CaiI CAGNNNCTG 2 cut(s) 605, 3314
CciI TCATGA 1 cut(s) 2410
CfoI GCGC 2 cut(s) 189, 2400
Cfr13I GGNCC 8 cut(s) 147, 619, 994, 1478, 2622, 2689, 2871, 3636
ClaI ATCGAT 1 cut(s) 3473
CseI GACGC 1 cut(s) 2522
Csp6I GTAC 5 cut(s) 720, 1005, 1486, 1670, 1967
CviQI GTAC 5 cut(s) 720, 1005, 1486, 1670, 1967
DraI TTTAAA 1 cut(s) 3285
DraIII CACNNNGTG 1 cut(s) 1013
EciI GGCGGA 1 cut(s) 2792
Ecl136II GAGCTC 1 cut(s) 2171
Eco130I CCWWGG 3 cut(s) 1733, 1919, 2625
Eco24I GRGCYC 2 cut(s) 38, 2173
Eco31I GGTCTC 1 cut(s) 535
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 4 cut(s) 147, 619, 994, 2871
Eco53kI GAGCTC 1 cut(s) 2171
Eco57I CTGAAG 7 cut(s) 432, 696, 894, 1359, 1626, 2123, 3276
Eco72I CACGTG 1 cut(s) 1682
Eco81I CCTNAGG 1 cut(s) 849
Eco88I CYCGRG 5 cut(s) 177, 443, 532, 2993, 3383
Eco91I GGTNACC 1 cut(s) 857
EcoICRI GAGCTC 1 cut(s) 2171
EcoNI CCTNNNNNAGG 1 cut(s) 852
EcoO109I RGGNCCY 1 cut(s) 1478
EcoO65I GGTNACC 1 cut(s) 857
EcoRI GAATTC 2 cut(s) 250, 2955
EcoRII CCWGG 2 cut(s) 2011, 2742
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 3 cut(s) 1733, 1919, 2625
EcoT22I ATGCAT 2 cut(s) 1699, 1720
EcoT38I GRGCYC 2 cut(s) 38, 2173
ErhI CCWWGG 3 cut(s) 1733, 1919, 2625
FaqI GGGAC 3 cut(s) 194, 248, 2884
FauI CCCGC 3 cut(s) 1695, 3200, 3552
FbaI TGATCA 1 cut(s) 3150
FblI GTMKAC 1 cut(s) 971
FokI GGATG 9 cut(s) 54, 339, 623, 1157, 1270, 1946, 3347, 3503, 3575
FriOI GRGCYC 2 cut(s) 38, 2173
FspBI CTAG 8 cut(s) 1169, 1248, 1443, 1722, 1734, 2156, 2211, 2570
GlaI GCGC 2 cut(s) 188, 2399
GsaI CCCAGC 1 cut(s) 199
GsuI CTGGAG 1 cut(s) 2765
HaeIII GGCC 5 cut(s) 1336, 1480, 2624, 2690, 3637
HapII CCGG 4 cut(s) 212, 235, 281, 3097
HgaI GACGC 1 cut(s) 2522
HhaI GCGC 2 cut(s) 189, 2400
Hin6I GCGC 2 cut(s) 187, 2398
HinP1I GCGC 2 cut(s) 187, 2398
HincII GTYRAC 2 cut(s) 1821, 2848
HindII GTYRAC 2 cut(s) 1821, 2848
HindIII AAGCTT 3 cut(s) 2292, 2405, 3287
HpaII CCGG 4 cut(s) 212, 235, 281, 3097
HphI GGTGA 7 cut(s) 661, 812, 869, 1517, 2001, 2336, 2867
Hpy166II GTNNAC 8 cut(s) 972, 1007, 1679, 1769, 1821, 2009, 2042, 2848
Hpy8I GTNNAC 8 cut(s) 972, 1007, 1679, 1769, 1821, 2009, 2042, 2848
Hpy99I CGWCG 1 cut(s) 817
HpyAV CCTTC 6 cut(s) 354, 1820, 1989, 2243, 2638, 3107
HpyCH4III ACNGT 7 cut(s) 11, 719, 817, 1331, 1773, 2039, 3508
HpyCH4IV ACGT 7 cut(s) 753, 1681, 1965, 2005, 2044, 2223, 2245
HpySE526I ACGT 7 cut(s) 753, 1681, 1965, 2005, 2044, 2223, 2245
HspAI GCGC 2 cut(s) 187, 2398
Ksp22I TGATCA 1 cut(s) 3150
LguI GCTCTTC 2 cut(s) 672, 1313
LmnI GCTCC 6 cut(s) 292, 1803, 1921, 2746, 3172, 3555
LweI GCATC 8 cut(s) 235, 370, 601, 1706, 1727, 1924, 2606, 3357
MaeI CTAG 8 cut(s) 1169, 1248, 1443, 1722, 1734, 2156, 2211, 2570
MaeII ACGT 7 cut(s) 753, 1681, 1965, 2005, 2044, 2223, 2245
MaeIII GTNAC 7 cut(s) 352, 857, 1067, 2241, 2917, 3268, 3508
MfeI CAATTG 1 cut(s) 900
MflI RGATCY 8 cut(s) 191, 439, 1237, 1597, 1778, 2834, 3613, 3642
MhlI GDGCHC 9 cut(s) 38, 289, 1057, 1308, 1681, 1687, 2044, 2137, 2173
MlyI GAGTC 2 cut(s) 1649, 2288
MmeI TCCRAC 1 cut(s) 125
Mph1103I ATGCAT 2 cut(s) 1699, 1720
MroXI GAANNNNTTC 4 cut(s) 2295, 2439, 2634, 2836
MslI CAYNNNNRTG 5 cut(s) 546, 1250, 2130, 3365, 3398
MspA1I CMGCKG 2 cut(s) 1102, 3458
MspI CCGG 4 cut(s) 212, 235, 281, 3097
MspR9I CCNGG 5 cut(s) 213, 236, 282, 2013, 2744
MunI CAATTG 1 cut(s) 900
Mva1269I GAATGC 2 cut(s) 1178, 2929
MvaI CCWGG 2 cut(s) 2013, 2744
NciI CCSGG 3 cut(s) 213, 236, 282
NcoI CCATGG 2 cut(s) 1919, 2625
NheI GCTAGC 1 cut(s) 1442
NlaIV GGNNCC 7 cut(s) 210, 487, 1479, 1780, 2872, 3174, 3638
NmeAIII GCCGAG 1 cut(s) 1813
NmuCI GTSAC 4 cut(s) 857, 1067, 3268, 3508
NsiI ATGCAT 2 cut(s) 1699, 1720
NspI RCATGY 2 cut(s) 1343, 2129
NspV TTCGAA 2 cut(s) 48, 725
OliI CACNNNNGTG 1 cut(s) 1250
PaeI GCATGC 1 cut(s) 1343
PaeR7I CTCGAG 3 cut(s) 443, 532, 3383
PagI TCATGA 1 cut(s) 2410
PciSI GCTCTTC 2 cut(s) 672, 1313
PcsI WCGNNNNNNNCGW 1 cut(s) 2002
PctI GAATGC 2 cut(s) 1178, 2929
PdmI GAANNNNTTC 4 cut(s) 2295, 2439, 2634, 2836
PfeI GAWTC 8 cut(s) 602, 896, 1126, 1559, 2414, 2946, 3343, 3470
PflMI CCANNNNNTGG 1 cut(s) 2698
PleI GAGTC 2 cut(s) 1648, 2287
PmaCI CACGTG 1 cut(s) 1682
PmlI CACGTG 1 cut(s) 1682
PpsI GAGTC 2 cut(s) 1648, 2287
Ppu21I YACGTR 3 cut(s) 1682, 2045, 2246
PsiI TTATAA 1 cut(s) 2058
Psp124BI GAGCTC 1 cut(s) 2173
Psp6I CCWGG 2 cut(s) 2011, 2742
PspCI CACGTG 1 cut(s) 1682
PspEI GGTNACC 1 cut(s) 857
PspFI CCCAGC 1 cut(s) 195
PspGI CCWGG 2 cut(s) 2011, 2742
PspN4I GGNNCC 7 cut(s) 210, 487, 1479, 1780, 2872, 3174, 3638
PspPI GGNCC 8 cut(s) 147, 619, 994, 1478, 2622, 2689, 2871, 3636
PstI CTGCAG 1 cut(s) 3186
PstNI CAGNNNCTG 2 cut(s) 605, 3314
PsuI RGATCY 8 cut(s) 191, 439, 1237, 1597, 1778, 2834, 3613, 3642
PvuII CAGCTG 1 cut(s) 3458
RsaI GTAC 5 cut(s) 721, 1006, 1487, 1671, 1968
RsaNI GTAC 5 cut(s) 720, 1005, 1486, 1670, 1967
RseI CAYNNNNRTG 5 cut(s) 546, 1250, 2130, 3365, 3398
SacI GAGCTC 1 cut(s) 2173
SapI GCTCTTC 2 cut(s) 672, 1313
Sau96I GGNCC 8 cut(s) 147, 619, 994, 1478, 2622, 2689, 2871, 3636
ScaI AGTACT 1 cut(s) 721
SchI GAGTC 2 cut(s) 1649, 2288
ScrFI CCNGG 5 cut(s) 213, 236, 282, 2013, 2744
SduI GDGCHC 9 cut(s) 38, 289, 1057, 1308, 1681, 1687, 2044, 2137, 2173
SfaNI GCATC 8 cut(s) 235, 370, 601, 1706, 1727, 1924, 2606, 3357
SfcI CTRYAG 3 cut(s) 89, 375, 3182
Sfr274I CTCGAG 3 cut(s) 443, 532, 3383
SfuI TTCGAA 2 cut(s) 48, 725
SinI GGWCC 4 cut(s) 147, 619, 994, 2871
SlaI CTCGAG 3 cut(s) 443, 532, 3383
SmiMI CAYNNNNRTG 5 cut(s) 546, 1250, 2130, 3365, 3398
SmlI CTYRAG 6 cut(s) 443, 532, 1409, 2304, 3383, 3572
SmoI CTYRAG 6 cut(s) 443, 532, 1409, 2304, 3383, 3572
SphI GCATGC 1 cut(s) 1343
SsiI CCGC 7 cut(s) 1102, 1337, 1688, 2803, 2941, 3193, 3559
SspMI CTAG 8 cut(s) 1169, 1248, 1443, 1722, 1734, 2156, 2211, 2570
SstI GAGCTC 1 cut(s) 2173
StyD4I CCNGG 5 cut(s) 211, 234, 280, 2011, 2742
StyI CCWWGG 3 cut(s) 1733, 1919, 2625
TaaI ACNGT 7 cut(s) 11, 719, 817, 1331, 1773, 2039, 3508
TaiI ACGT 7 cut(s) 756, 1684, 1968, 2008, 2047, 2226, 2248
TaqII GACCGA 1 cut(s) 2833
TatI WGTACW 1 cut(s) 719
TauI GCSGC 2 cut(s) 1105, 1339
TfiI GAWTC 8 cut(s) 602, 896, 1126, 1559, 2414, 2946, 3343, 3470
TscAI CASTG 4 cut(s) 16, 172, 888, 3315
TseFI GTSAC 4 cut(s) 857, 1067, 3268, 3508
Tsp45I GTSAC 4 cut(s) 857, 1067, 3268, 3508
TspGWI ACGGA 2 cut(s) 1871, 2515
TspRI CASTG 4 cut(s) 16, 172, 888, 3315
Van91I CCANNNNNTGG 1 cut(s) 2698
VneI GTGCAC 2 cut(s) 1677, 2040
VpaK11BI GGWCC 4 cut(s) 147, 619, 994, 2871
XagI CCTNNNNNAGG 1 cut(s) 852
XceI RCATGY 2 cut(s) 1343, 2129
XcmI CCANNNNNNNNNTGG 2 cut(s) 1295, 3560
XhoI CTCGAG 3 cut(s) 443, 532, 3383
XmaJI CCTAGG 1 cut(s) 1733
XmiI GTMKAC 1 cut(s) 971
XmnI GAANNNNTTC 4 cut(s) 2295, 2439, 2634, 2836
XspI CTAG 8 cut(s) 1169, 1248, 1443, 1722, 1734, 2156, 2211, 2570
ZrmI AGTACT 1 cut(s) 721
Zsp2I ATGCAT 2 cut(s) 1699, 1720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.