Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0005339

Physical Location & Seq

Genomic Coordinates Forward (+)

8903457 .. 8907716

Gene Structure 4260 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g15730.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2565 bp

ATGGCACAAAATTTGTTGCGAGCAAAGCATTTAAGTAACCTCTCGAGTAGTGCTAGATCCTTTTTTATTAGCGGGTCACGATGTAGTGCAACAGAGGGAAATTCATGCACTTGCTCTGAAGATGAAAACTGTGGCCCACAACGGCAGCAAGCAATAAATAGAGGTCTTCTTGCCCAAAATCCATCAAGCTCCGTATCTAAACCTGCTGCAGGGGCAGGAATCTTAATTTCGCAAGATGCAGTGAAAGTGGCAGATTCTGGTAAATCCAAGAGTGTTGACCAGAGAACTTCTATAAAACAGGTGGCAACTGCCCCAACACCTTTTGTGAGAGCAGATACTGTAAGTTATGCTACCAACGTTGATGCCATTCAGAAGGATATAAGCTCTTCACCTCCTACTACAGAGCAATTTGTTAAGGCTGGCGTCGCCGCTGTCAATTTTTTGTCAGATATAGTTAGTTATAAGATTCCCTTGTCTGATGGGATGGGAATGCTTACTTTGCCGAAAAACACTATGGTTCGTCCCACCGTGGGAGTGTCCAGTGTCAAGTCATCAAATGTGAAGCAGATAAACAGAGAGAATTTCATTAGTGTTCATCCAAAACCATCTACTGAGACAGCAGCGGCTTCAGAGCGTACAAGTAATCATCAGGGGTCAAAGGGAAATTATGATAAATCCAATTCTGTTAAAGGTCTAAATCATGTTCCATATACCAGGACAGAAAACTCAGCAGTGGCTCACAGTGTACAGACTTTAGAAACACGTGATAGAAGGGCTTTGCCACGTAAATCAAAAGCTCAGCCAAACCACTTCGTTCCAGATTTCAAGTCCAATGTACAGATTTCAGATGCAGAGACTACGAGGTGTGGCTCTAAAGGTTTCAGTAAACCTGTACGAGAGATGAAAATGCCAACAGCGATTGCTCCATTTAATAGGCAGTTTGTGCACAATGGGAATGTTGTTCAAAATGTTTCCCACATATTGCAACAGCTTAAATGGGGCCCTTCTGCAGAGGCATCTCTCAGAAATCTCAACTGTTCAATGGACGCATATCAAGCAAACCAAATTTTGAAGCAACTTCAAGATCATACTGTTGCACTTGGTTTTTTTAATTGGTTAAAACGACAAGCAGGATTCAGGCATGATGGGCACACTTACACCACCATGGTTGGCATCCTTGGTCGTGCCAGGCAGTTCGGTGCCATCAATAAATTGCTCAATCAGATGGTGAATGAAGGATGTCAACCAAATGTTGTGACATACAACCGTCTGATTCATAGTTATGGTCGTGCAAACTATTTGAAAGATGCAATGAATGTGTTCAGTCAAATGCAAGAAGCAGGGTGTGAGCCTGACCGTGTCACCTACTGTACACTAATTGACATCCATGCAAAATCTGGTTTTCTTGATATTGCCCTTGGACTGTATGATAGGATGCAAAAGGCTGGTCTTTCGCCTGACACATTTACTTACAGTGTTATGATCAACTGCCTCGGGAAAGCTGGCCATTTAGCTGCCGCTCACCGGCTGTTCTGTGAGATGGTGGATCATGGTTGTGTTCCCAATCTGGTCACCTATAATATCATGATAGCTTTGCAGGCGAAAGCACGGAATTATGAGACTGCGTTGAAGCTCTACCGTGACATGCAGGGTGCTGGATTTCAGCCAGATAAAGTGACTTATAGCATAGTAATGGAGGTGCTTGGTCACTGTGGGTATCTTGAGGAGGCCGAAGCACTTTTTGGTGAAATGAAACGGAAGAATTGGGTACCTGATGAACCTGTTTATGGCCTTTTAGTTGATTTGTGGGGCAAGGCTGGTAATGTGCAAAAAGCGTGGGATTGGTATCAAGCAATGCTCCATGCTGGTTTGCGTCCTAATAGCATGGTGGGCTTAGGTCTGAACCCTTCTTTGCAGACATATACCTTGCTTCTCAGTTGTTGCACGGAAGCACAATCACCATATGACATGGAATTTTGTTGTGAGCTCATGGCAGTCACACGTCATCCTGCTCATACATTCCTGCTGTCTATGCCCTCAGCAGGGCCCGATGGCCAAAACGTGAGAGAACACATGAACAGCTTCTTGGATTTGATGCATAGTGAGGATAGAGAAAGCAAGAGGGGGCTTGTAGATGCAGTAGTGGATTTCCTTCATAAATCAGGGCTCAAGGAGGAGGCTGGTTCTGTCTGGGAGGTGGCTGCGCAGAAGAATGTTTATCCAGACGCTGTCAAAGAGAAGGGCTCATGTTATTGGCTTATAAACCTACATGTTATGTCTGATGGTACTGCTGTGACAGCACTATCAAGGACACTTGCTTGGTTTCGCCAACAAATGCTAATATCCGGGGTTTGTCCAAACCGGATTGATATTGTGACAGGATGGGGACGGCGGAGTAGGGTTACAGGAACTTCTATGGTGAGGCATGCAGTGCAGGAACTGCTGCACATGTTTAGCTTCCCGTTTTTCACCGAGAATGGGAATTCTGGGTGCTTTGTAGGATGTGGGGAGTCTCTTAATAGATGGTTGCTTGAATCTTATGTGGAGCGGATGCATTTATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

855

Amino Acids

94.32

Weight (kDa)

8.8

Isoelectric Point (pI)

34.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	377 - 429	8.9e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	378 - 467	9.8e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	384 - 426	4.3e-09	PPR repeat family
PPR_3	PF13812	404 - 462	1.2e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	413 - 445	1.1e-10	PPR repeat
PPR_2	PF13041	416 - 464	7e-16	PPR repeat family
PPR	PF01535	419 - 449	7.8e-07	PPR repeat
PPR_3	PF13812	440 - 500	9.3e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	448 - 480	2e-06	PPR repeat
PPR_2	PF13041	458 - 500	1.2e-09	PPR repeat family
TPR_24	PF23276	461 - 583	6e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	469 - 572	2.1e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	476 - 532	3.6e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	483 - 515	1.5e-08	PPR repeat
PPR	PF01535	489 - 519	1.7e-06	PPR repeat
PPR_2	PF13041	494 - 530	6.3e-11	PPR repeat family
PPR_3	PF13812	509 - 569	1.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	521 - 568	3.5e-13	PPR repeat family
PPR	PF01535	559 - 588	4.6e-06	PPR repeat
PPR_3	PF13812	619 - 673	8.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	626 - 673	5e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005339)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18900 AT1G18900 AT1G18900 AT1G18900
fragaria_vesca	FvH4_5g15730 FvH4_5g15730 FvH4_5g15730
malus_domestica	MD06G1185900.v1.1 MD14G1192100.v1.1
prunus_persica	Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1
pyrus_communis	pycom06g16530 pycom14g15980
rosa_chinensis	RchiOBHm_Chr7g0180381
rosa_laevigata	RLG00000005272
rosa_multiflora	Rmu_sc0003526.1_g000009
rosa_roxburghii	Rroxscaffold_3G00272940
rosa_rugosa	Rorug06G0432600 Rorug06G0432700 Rorug06G0432800 Rorug06G0432800
rosa_samantha	Rh7AG033200 Rh7BG033100 Rh7CG035000 Rh7DG033600
rosa_wichuraiana	Rw7G002770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	462, 2261
Acc16I	TGCGCA	1 cut(s)	2205
Acc36I	ACCTGC	1 cut(s)	211
Acc65I	GGTACC	1 cut(s)	1768
AccB1I	GGYRCC	2 cut(s)	1199, 1768
AccBSI	CCGCTC	2 cut(s)	1520, 2548
AciI	CCGC	6 cut(s)	72, 429, 623, 1518, 2392, 2548
AclI	AACGTT	1 cut(s)	357
AclWI	GGATC	2 cut(s)	51, 1554
AcoI	YGGCCR	2 cut(s)	1504, 2053
AcsI	RAATTY	6 cut(s)	10, 100, 580, 1065, 1973, 2482
AcuI	CTGAAG	2 cut(s)	138, 612
AcvI	CACGTG	1 cut(s)	764
AcyI	GRCGYC	1 cut(s)	423
AfaI	GTAC	7 cut(s)	637, 747, 837, 894, 1372, 1770, 2287
AfiI	CCNNNNNNNGG	7 cut(s)	209, 530, 1524, 1787, 2042, 2043, 2478
AflIII	ACRYGT	4 cut(s)	761, 2000, 2269, 2448
AgsI	TTSAA	8 cut(s)	826, 965, 1041, 1072, 1082, 1303, 1630, 2534
AjiI	CACGTC	1 cut(s)	2003
AjnI	CCWGG	2 cut(s)	713, 1187
AjuI	GAANNNNNNNTTGG	4 cut(s)	1725, 1757, 2069, 2101
Alw21I	GWGCWC	2 cut(s)	948, 1989
Alw26I	GTCTC	4 cut(s)	608, 848, 1613, 2517
Alw44I	GTGCAC	1 cut(s)	944
AlwI	GGATC	2 cut(s)	51, 1554
AlwNI	CAGNNNCTG	4 cut(s)	257, 338, 2228, 2440
Ama87I	CYCGRG	2 cut(s)	43, 1493
AoxI	GGCC	7 cut(s)	133, 1000, 1504, 1728, 1789, 2045, 2053
ApaI	GGGCCC	2 cut(s)	1004, 2049
ApaLI	GTGCAC	1 cut(s)	944
ApeKI	GCWGC	6 cut(s)	145, 206, 620, 1514, 2201, 2443
ApoI	RAATTY	6 cut(s)	10, 100, 580, 1065, 1973, 2482
ArsI	GACNNNNNNTTYG	2 cut(s)	1432, 1464
Asp700I	GAANNNNTTC	2 cut(s)	1319, 2081
Asp718I	GGTACC	1 cut(s)	1768
AspLEI	GCGC	1 cut(s)	2206
AspS9I	GGNCC	5 cut(s)	134, 1000, 1001, 2045, 2046
AsuC2I	CCSGG	1 cut(s)	2347
AsuHPI	GGTGA	9 cut(s)	381, 1240, 1354, 1514, 1564, 1757, 1950, 2431, 2461
AvaI	CYCGRG	2 cut(s)	43, 1493
BaeGI	GKGCMC	4 cut(s)	948, 1004, 1152, 2049
BaeI	ACNNNNGTAYC	2 cut(s)	2277, 2310
BalI	TGGCCA	2 cut(s)	1506, 2055
BanI	GGYRCC	2 cut(s)	1199, 1768
BanII	GRGCYC	5 cut(s)	1004, 1989, 2049, 2169, 2246
BbrPI	CACGTG	1 cut(s)	764
BbsI	GAAGAC	1 cut(s)	158
Bbv12I	GWGCWC	2 cut(s)	948, 1989
BbvCI	CCTCAGC	1 cut(s)	2038
BbvI	GCAGC	6 cut(s)	157, 193, 632, 1501, 2188, 2430
BceAI	ACGGC	2 cut(s)	158, 2405
BciT130I	CCWGG	2 cut(s)	715, 1189
BclI	TGATCA	1 cut(s)	1482
BcnI	CCSGG	1 cut(s)	2347
BcoDI	GTCTC	4 cut(s)	608, 848, 1613, 2517
BfaI	CTAG	1 cut(s)	54
BfmI	CTRYAG	3 cut(s)	207, 399, 1008
BfuAI	ACCTGC	1 cut(s)	211
BisI	GCNGC	9 cut(s)	146, 207, 429, 621, 624, 1515, 1518, 2202, 2444
BlpI	GCTNAGC	1 cut(s)	798
BlsI	GCNGC	9 cut(s)	147, 208, 430, 622, 625, 1516, 1519, 2203, 2445
Bme1390I	CCNGG	3 cut(s)	715, 1189, 2347
BmeT110I	CYCGRG	2 cut(s)	43, 1493
BmgBI	CACGTC	1 cut(s)	2003
BmgT120I	GGNCC	5 cut(s)	134, 1000, 1001, 2045, 2046
BmiI	GGNNCC	5 cut(s)	1001, 1002, 1201, 1770, 2047
BmrFI	CCNGG	3 cut(s)	715, 1189, 2347
BpiI	GAAGAC	1 cut(s)	158
BplI	GAGNNNNNCTC	2 cut(s)	2228, 2260
Bpu10I	CCTNAGC	2 cut(s)	1894, 2038
Bpu1102I	GCTNAGC	1 cut(s)	798
BpuEI	CTTGAG	2 cut(s)	1742, 2153
BpuMI	CCSGG	1 cut(s)	2347
BsaAI	YACGTR	2 cut(s)	764, 785
BsaBI	GATNNNNATC	1 cut(s)	1845
BsaHI	GRCGYC	1 cut(s)	423
BsaJI	CCNNGG	6 cut(s)	528, 1164, 1177, 1417, 1492, 2346
BsaWI	WCCGGW	1 cut(s)	2361
Bsc4I	CCNNNNNNNGG	7 cut(s)	209, 530, 1524, 1787, 2042, 2043, 2478
Bse118I	RCCGGY	1 cut(s)	1524
Bse1I	ACTGG	1 cut(s)	540
Bse3DI	GCAATG	2 cut(s)	1317, 1860
Bse8I	GATNNNNATC	1 cut(s)	1845
BseBI	CCWGG	2 cut(s)	715, 1189
BseDI	CCNNGG	6 cut(s)	528, 1164, 1177, 1417, 1492, 2346
BseJI	GATNNNNATC	1 cut(s)	1845
BseLI	CCNNNNNNNGG	7 cut(s)	209, 530, 1524, 1787, 2042, 2043, 2478
BseMI	GCAATG	2 cut(s)	1317, 1860
BseMII	CTCAG	6 cut(s)	603, 741, 812, 1036, 1948, 2052
BseNI	ACTGG	1 cut(s)	540
BseRI	GAGGAG	2 cut(s)	1739, 2189
BseSI	GKGCMC	4 cut(s)	948, 1004, 1152, 2049
BseXI	GCAGC	6 cut(s)	157, 193, 632, 1501, 2188, 2430
BsgI	GTGCAG	2 cut(s)	2429, 2453
BshFI	GGCC	7 cut(s)	135, 1002, 1506, 1730, 1791, 2047, 2055
BshNI	GGYRCC	2 cut(s)	1199, 1768
BsiHKAI	GWGCWC	2 cut(s)	948, 1989
BsiHKCI	CYCGRG	2 cut(s)	43, 1493
BsiSI	CCGG	3 cut(s)	1525, 2346, 2362
BslFI	GGGAC	2 cut(s)	507, 2400
BslI	CCNNNNNNNGG	7 cut(s)	209, 530, 1524, 1787, 2042, 2043, 2478
BsmAI	GTCTC	4 cut(s)	608, 848, 1613, 2517
BsmFI	GGGAC	2 cut(s)	507, 2400
BsmI	GAATGC	1 cut(s)	495
BsnI	GGCC	7 cut(s)	135, 1002, 1506, 1730, 1791, 2047, 2055
BsoBI	CYCGRG	2 cut(s)	43, 1493
Bsp120I	GGGCCC	2 cut(s)	1000, 2045
Bsp1286I	GDGCHC	7 cut(s)	948, 1004, 1152, 1989, 2049, 2169, 2246
Bsp1407I	TGTACA	3 cut(s)	745, 835, 1370
Bsp143I	GATC	4 cut(s)	56, 1084, 1482, 1546
Bsp1720I	GCTNAGC	1 cut(s)	798
Bsp19I	CCATGG	1 cut(s)	1164
BspACI	CCGC	6 cut(s)	72, 429, 623, 1518, 2392, 2548
BspANI	GGCC	7 cut(s)	135, 1002, 1506, 1730, 1791, 2047, 2055
BspCNI	CTCAG	6 cut(s)	604, 740, 811, 1035, 1947, 2051
BspHI	TCATGA	1 cut(s)	1584
BspLI	GGNNCC	5 cut(s)	1001, 1002, 1201, 1770, 2047
BspMAI	CTGCAG	2 cut(s)	211, 1012
BspMI	ACCTGC	1 cut(s)	211
BspPI	GGATC	2 cut(s)	51, 1554
BspQI	GCTCTTC	1 cut(s)	391
BspT107I	GGYRCC	2 cut(s)	1199, 1768
BsrBI	CCGCTC	2 cut(s)	1520, 2548
BsrDI	GCAATG	2 cut(s)	1317, 1860
BsrFI	RCCGGY	1 cut(s)	1524
BsrGI	TGTACA	3 cut(s)	745, 835, 1370
BsrI	ACTGG	1 cut(s)	540
BssAI	RCCGGY	1 cut(s)	1524
BssECI	CCNNGG	6 cut(s)	528, 1164, 1177, 1417, 1492, 2346
BssMI	GATC	4 cut(s)	56, 1084, 1482, 1546
BssNI	GRCGYC	1 cut(s)	423
BssT1I	CCWWGG	3 cut(s)	1164, 1177, 1417
Bst2UI	CCWGG	2 cut(s)	715, 1189
Bst6I	CTCTTC	1 cut(s)	391
BstACI	GRCGYC	1 cut(s)	423
BstAPI	GCANNNNNTGC	3 cut(s)	943, 2431, 2440
BstAUI	TGTACA	3 cut(s)	745, 835, 1370
BstBAI	YACGTR	2 cut(s)	764, 785
BstC8I	GCNNGC	6 cut(s)	21, 150, 421, 1504, 1599, 2427
BstDEI	CTNAG	7 cut(s)	612, 727, 798, 1022, 1894, 1934, 2038
BstDSI	CCRYGG	2 cut(s)	528, 1164
BstEII	GGTNACC	1 cut(s)	1570
BstENI	CCTNNNNNAGG	1 cut(s)	207
BstHHI	GCGC	1 cut(s)	2206
BstKTI	GATC	4 cut(s)	59, 1087, 1485, 1549
BstMAI	GTCTC	4 cut(s)	608, 848, 1613, 2517
BstMBI	GATC	4 cut(s)	56, 1084, 1482, 1546
BstNI	CCWGG	2 cut(s)	715, 1189
BstNSI	RCATGY	4 cut(s)	1648, 2273, 2429, 2452
BstPI	GGTNACC	1 cut(s)	1570
BstSCI	CCNGG	3 cut(s)	713, 1187, 2345
BstSFI	CTRYAG	3 cut(s)	207, 399, 1008
BstSLI	GKGCMC	4 cut(s)	948, 1004, 1152, 2049
BstV1I	GCAGC	6 cut(s)	157, 193, 632, 1501, 2188, 2430
BstV2I	GAAGAC	1 cut(s)	158
BstX2I	RGATCY	1 cut(s)	56
BstYI	RGATCY	1 cut(s)	56
BsuRI	GGCC	7 cut(s)	135, 1002, 1506, 1730, 1791, 2047, 2055
BtgI	CCRYGG	2 cut(s)	528, 1164
BtrI	CACGTC	1 cut(s)	2003
BtsI	GCAGTG	3 cut(s)	246, 738, 2436
BtsIMutI	CAGTG	7 cut(s)	246, 547, 738, 748, 1480, 1708, 2436
BveI	ACCTGC	1 cut(s)	211
Cac8I	GCNNGC	6 cut(s)	21, 150, 421, 1504, 1599, 2427
CaiI	CAGNNNCTG	4 cut(s)	257, 338, 2228, 2440
CciI	TCATGA	1 cut(s)	1584
CfoI	GCGC	1 cut(s)	2206
Cfr10I	RCCGGY	1 cut(s)	1524
Cfr13I	GGNCC	5 cut(s)	134, 1000, 1001, 2045, 2046
CseI	GACGC	4 cut(s)	412, 1055, 1862, 2234
Csp6I	GTAC	7 cut(s)	636, 746, 836, 893, 1371, 1769, 2286
CspCI	CAANNNNNGTGG	4 cut(s)	1150, 1185, 1817, 1852
CviQI	GTAC	7 cut(s)	636, 746, 836, 893, 1371, 1769, 2286
DdeI	CTNAG	7 cut(s)	612, 727, 798, 1022, 1894, 1934, 2038
DpnI	GATC	4 cut(s)	58, 1086, 1484, 1548
DpnII	GATC	4 cut(s)	56, 1084, 1482, 1546
EaeI	YGGCCR	2 cut(s)	1504, 2053
Eam1104I	CTCTTC	1 cut(s)	391
EarI	CTCTTC	1 cut(s)	391
EciI	GGCGGA	1 cut(s)	2407
Ecl136II	GAGCTC	1 cut(s)	1987
Eco130I	CCWWGG	3 cut(s)	1164, 1177, 1417
Eco24I	GRGCYC	5 cut(s)	1004, 1989, 2049, 2169, 2246
Eco53kI	GAGCTC	1 cut(s)	1987
Eco57I	CTGAAG	2 cut(s)	138, 612
Eco72I	CACGTG	1 cut(s)	764
Eco88I	CYCGRG	2 cut(s)	43, 1493
Eco91I	GGTNACC	1 cut(s)	1570
EcoICRI	GAGCTC	1 cut(s)	1987
EcoNI	CCTNNNNNAGG	1 cut(s)	207
EcoO109I	RGGNCCY	3 cut(s)	1000, 1001, 2045
EcoO65I	GGTNACC	1 cut(s)	1570
EcoRI	GAATTC	1 cut(s)	2482
EcoRII	CCWGG	2 cut(s)	713, 1187
EcoT14I	CCWWGG	3 cut(s)	1164, 1177, 1417
EcoT22I	ATGCAT	2 cut(s)	2100, 2556
EcoT38I	GRGCYC	5 cut(s)	1004, 1989, 2049, 2169, 2246
ErhI	CCWWGG	3 cut(s)	1164, 1177, 1417
FalI	AAGNNNNNCTT	2 cut(s)	455, 487
FaqI	GGGAC	2 cut(s)	507, 2400
FauI	CCCGC	1 cut(s)	65
FauNDI	CATATG	1 cut(s)	1963
FbaI	TGATCA	1 cut(s)	1482
Fnu4HI	GCNGC	9 cut(s)	146, 207, 429, 621, 624, 1515, 1518, 2202, 2444
FokI	GGATG	9 cut(s)	496, 582, 1160, 1251, 1370, 1447, 1992, 2394, 2514
FriOI	GRGCYC	5 cut(s)	1004, 1989, 2049, 2169, 2246
Fsp4HI	GCNGC	9 cut(s)	146, 207, 429, 621, 624, 1515, 1518, 2202, 2444
FspBI	CTAG	1 cut(s)	54
FspI	TGCGCA	1 cut(s)	2205
GlaI	GCGC	1 cut(s)	2205
GluI	GCNGC	9 cut(s)	146, 207, 429, 621, 624, 1515, 1518, 2202, 2444
HaeIII	GGCC	7 cut(s)	135, 1002, 1506, 1730, 1791, 2047, 2055
HapII	CCGG	3 cut(s)	1525, 2346, 2362
HgaI	GACGC	4 cut(s)	412, 1055, 1862, 2234
HhaI	GCGC	1 cut(s)	2206
Hin1I	GRCGYC	1 cut(s)	423
Hin6I	GCGC	1 cut(s)	2204
HinP1I	GCGC	1 cut(s)	2204
HincII	GTYRAC	2 cut(s)	277, 1244
HindII	GTYRAC	2 cut(s)	277, 1244
HinfI	GANTC	7 cut(s)	219, 254, 466, 1134, 1273, 2510, 2534
HpaII	CCGG	3 cut(s)	1525, 2346, 2362
HphI	GGTGA	9 cut(s)	381, 1240, 1354, 1514, 1564, 1757, 1950, 2431, 2461
Hpy166II	GTNNAC	6 cut(s)	277, 746, 887, 946, 1244, 1373
Hpy188III	TCNNGA	9 cut(s)	43, 78, 818, 1082, 1406, 1495, 1585, 1721, 2222
Hpy8I	GTNNAC	6 cut(s)	277, 746, 887, 946, 1244, 1373
Hpy99I	CGWCG	1 cut(s)	428
HpyAV	CCTTC	7 cut(s)	367, 765, 1014, 1229, 1917, 2162, 2233
HpyCH4IV	ACGT	5 cut(s)	357, 763, 784, 2002, 2061
HpyF3I	CTNAG	7 cut(s)	612, 727, 798, 1022, 1894, 1934, 2038
HpySE526I	ACGT	5 cut(s)	357, 763, 784, 2002, 2061
Hsp92I	GRCGYC	1 cut(s)	423
HspAI	GCGC	1 cut(s)	2204
KpnI	GGTACC	1 cut(s)	1772
Ksp22I	TGATCA	1 cut(s)	1482
Kzo9I	GATC	4 cut(s)	56, 1084, 1482, 1546
LguI	GCTCTTC	1 cut(s)	391
LmnI	GCTCC	4 cut(s)	194, 928, 1863, 2545
Lsp1109I	GCAGC	6 cut(s)	157, 193, 632, 1501, 2188, 2430
MaeI	CTAG	1 cut(s)	54
MaeII	ACGT	5 cut(s)	357, 763, 784, 2002, 2061
MalI	GATC	4 cut(s)	58, 1086, 1484, 1548
MbiI	CCGCTC	2 cut(s)	1520, 2548
MboI	GATC	4 cut(s)	56, 1084, 1482, 1546
MboII	GAAGA	5 cut(s)	131, 158, 378, 1771, 2221
MflI	RGATCY	1 cut(s)	56
MhlI	GDGCHC	7 cut(s)	948, 1004, 1152, 1989, 2049, 2169, 2246
MlsI	TGGCCA	2 cut(s)	1506, 2055
MluNI	TGGCCA	2 cut(s)	1506, 2055
MlyI	GAGTC	1 cut(s)	2519
Mox20I	TGGCCA	2 cut(s)	1506, 2055
Mph1103I	ATGCAT	2 cut(s)	2100, 2556
MroXI	GAANNNNTTC	2 cut(s)	1319, 2081
MscI	TGGCCA	2 cut(s)	1506, 2055
MseI	TTAA	9 cut(s)	32, 224, 414, 687, 930, 993, 1110, 1118, 2517
MslI	CAYNNNNRTG	4 cut(s)	1163, 1281, 1554, 1691
Msp20I	TGGCCA	2 cut(s)	1506, 2055
MspA1I	CMGCKG	2 cut(s)	431, 623
MspI	CCGG	3 cut(s)	1525, 2346, 2362
MspR9I	CCNGG	3 cut(s)	715, 1189, 2347
Mva1269I	GAATGC	1 cut(s)	495
MvaI	CCWGG	2 cut(s)	715, 1189
NciI	CCSGG	1 cut(s)	2347
NcoI	CCATGG	1 cut(s)	1164
NdeI	CATATG	1 cut(s)	1963
NdeII	GATC	4 cut(s)	56, 1084, 1482, 1546
NlaIV	GGNNCC	5 cut(s)	1001, 1002, 1201, 1770, 2047
NsbI	TGCGCA	1 cut(s)	2205
NsiI	ATGCAT	2 cut(s)	2100, 2556
NspI	RCATGY	4 cut(s)	1648, 2273, 2429, 2452
PaeI	GCATGC	1 cut(s)	2429
PaeR7I	CTCGAG	1 cut(s)	43
PagI	TCATGA	1 cut(s)	1584
PciI	ACATGT	2 cut(s)	2269, 2448
PciSI	GCTCTTC	1 cut(s)	391
PctI	GAATGC	1 cut(s)	495
PdmI	GAANNNNTTC	2 cut(s)	1319, 2081
PfeI	GAWTC	6 cut(s)	219, 254, 466, 1134, 1273, 2534
PflFI	GACNNNGTC	2 cut(s)	1358, 2228
PkrI	GCNGC	9 cut(s)	147, 208, 430, 622, 625, 1516, 1519, 2203, 2445
PleI	GAGTC	1 cut(s)	2518
PmaCI	CACGTG	1 cut(s)	764
PmlI	CACGTG	1 cut(s)	764
PpsI	GAGTC	1 cut(s)	2518
Ppu21I	YACGTR	2 cut(s)	764, 785
PscI	ACATGT	2 cut(s)	2269, 2448
PsiI	TTATAA	2 cut(s)	462, 2261
Psp124BI	GAGCTC	1 cut(s)	1989
Psp1406I	AACGTT	1 cut(s)	357
Psp6I	CCWGG	2 cut(s)	713, 1187
PspCI	CACGTG	1 cut(s)	764
PspEI	GGTNACC	1 cut(s)	1570
PspGI	CCWGG	2 cut(s)	713, 1187
PspN4I	GGNNCC	5 cut(s)	1001, 1002, 1201, 1770, 2047
PspOMI	GGGCCC	2 cut(s)	1000, 2045
PspPI	GGNCC	5 cut(s)	134, 1000, 1001, 2045, 2046
PstI	CTGCAG	2 cut(s)	211, 1012
PstNI	CAGNNNCTG	4 cut(s)	257, 338, 2228, 2440
PsuI	RGATCY	1 cut(s)	56
PsyI	GACNNNGTC	2 cut(s)	1358, 2228
RsaI	GTAC	7 cut(s)	637, 747, 837, 894, 1372, 1770, 2287
RsaNI	GTAC	7 cut(s)	636, 746, 836, 893, 1371, 1769, 2286
RseI	CAYNNNNRTG	4 cut(s)	1163, 1281, 1554, 1691
SacI	GAGCTC	1 cut(s)	1989
SapI	GCTCTTC	1 cut(s)	391
SaqAI	TTAA	9 cut(s)	32, 224, 414, 687, 930, 993, 1110, 1118, 2517
SatI	GCNGC	9 cut(s)	146, 207, 429, 621, 624, 1515, 1518, 2202, 2444
Sau3AI	GATC	4 cut(s)	56, 1084, 1482, 1546
Sau96I	GGNCC	5 cut(s)	134, 1000, 1001, 2045, 2046
SchI	GAGTC	1 cut(s)	2519
ScrFI	CCNGG	3 cut(s)	715, 1189, 2347
SduI	GDGCHC	7 cut(s)	948, 1004, 1152, 1989, 2049, 2169, 2246
SfcI	CTRYAG	3 cut(s)	207, 399, 1008
Sfr274I	CTCGAG	1 cut(s)	43
SlaI	CTCGAG	1 cut(s)	43
SmiMI	CAYNNNNRTG	4 cut(s)	1163, 1281, 1554, 1691
SmlI	CTYRAG	3 cut(s)	43, 1721, 2168
SmoI	CTYRAG	3 cut(s)	43, 1721, 2168
SphI	GCATGC	1 cut(s)	2429
SsiI	CCGC	6 cut(s)	72, 429, 623, 1518, 2392, 2548
SspMI	CTAG	1 cut(s)	54
SstI	GAGCTC	1 cut(s)	1989
StyD4I	CCNGG	3 cut(s)	713, 1187, 2345
StyI	CCWWGG	3 cut(s)	1164, 1177, 1417
TaiI	ACGT	5 cut(s)	360, 766, 787, 2005, 2064
TaqI	TCGA	1 cut(s)	44
TatI	WGTACW	3 cut(s)	745, 835, 1370
TauI	GCSGC	3 cut(s)	431, 626, 1520
TfiI	GAWTC	6 cut(s)	219, 254, 466, 1134, 1273, 2534
Tru1I	TTAA	9 cut(s)	32, 224, 414, 687, 930, 993, 1110, 1118, 2517
Tru9I	TTAA	9 cut(s)	32, 224, 414, 687, 930, 993, 1110, 1118, 2517
TscAI	CASTG	7 cut(s)	246, 547, 738, 748, 1480, 1715, 2436
TseI	GCWGC	6 cut(s)	145, 206, 620, 1514, 2201, 2443
TspGWI	ACGGA	4 cut(s)	181, 1624, 1771, 1961
TspRI	CASTG	7 cut(s)	246, 547, 738, 748, 1480, 1715, 2436
Tth111I	GACNNNGTC	2 cut(s)	1358, 2228
VneI	GTGCAC	1 cut(s)	944
XagI	CCTNNNNNAGG	1 cut(s)	207
XapI	RAATTY	6 cut(s)	10, 100, 580, 1065, 1973, 2482
XceI	RCATGY	4 cut(s)	1648, 2273, 2429, 2452
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1394
XhoI	CTCGAG	1 cut(s)	43
XmnI	GAANNNNTTC	2 cut(s)	1319, 2081
XspI	CTAG	1 cut(s)	54
Zsp2I	ATGCAT	2 cut(s)	2100, 2556

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.