Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0005339

Physical Location & Seq

Genomic Coordinates Reverse (-)

2188930 .. 2193466

Gene Structure 4537 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG033100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2640 bp

ATGGCACAAAATTTGTTGCGCGCAAAGCATATAGGTAACCTCTCAAGTAGTGCTAGGTCATTTTTTCTCAGCGGGTCACGATGTAGTGCAACAGAGGGAAATTCATGCACTTGCTCTGAAGATGAAAACTGTGCCCCACAAAGGCAGCAAGCAAGAAATAGAGGTCTTCTTGCCCAAAATCCATCAAGCACCGGATCTAAACCTTCTGCAAGGGCAGGAATCTTAGTTTCGGGAGATGCAGTGAAAGTAGCAGACTCTCGTAAATCTGAGAGTGTTGACCAGACAACTTCTATAAAACAGGTGGCAACTGCCCCAACACCTTTTGTGAGATCAGGTACTGTAAGTTATGCTAGCAACGTTGATGGCATTCAGAAGGATGTAAGCTCATCACCTCTTGCTACAGAGCAATTTGTTAAGGCTGGTGTTGCAGCTGTCAATTTTTTGTCAGATATAGTGAGTTATAAGATTCCTTTGTCTGATGGGATGGGGATGCTTAGTTTGCCAAAAAACACTGTGGTTCGTTCCACCGTGGGAGTCTCCAGTGTCAAATCATCAAATGTGAAGCAGATAAACAGAGAGCATTTCACTAGTGTGCATCCAAAGCCATCTACTGAGAGAGCAGCAGCTTCAGAGCGTACGAGTAATCATCAGGGAACAAAGGGAAATGTTGATAAATCCAGTTCTGTTAGAGGTCTAAATCATGTTCCATATAACAGGACAGAAAACTCAGCAGCGGCTCACAGTGTTCAGACTCCAGAAGCTCATGATAGAAGGGCTTTGCCACGAAAATCAAAAGCTCAGCCAAACCACTTCGTTCCAGATTTCAGTTCCAATGTACAGATTTCAGATACAGAGACTACTAGATGTGGCCCTAAAGGTTTCAATAAACCTGCAAGAGAGATGAAAATGCCAACAGGGATTGCTCCATTCAACAGGCAGTGTGCGCACAAGGGGAATGTTGTTCAAAATGTTTCTCACATACTGCAACAGCTTAAATGGGGCCCTTCTGCAGAGGCAGCTCTTGGAAGTCTCAACTGTTCAATGGACGCATATCAAGCCAACCAAATTTTGAAGCAAATTCAAGATCATTCTGTTGCTCTTGGATTTTTTAATTGGTTAAAACGACAAGCAGGATTCAGGCATGATGGGCACACTTACACCACCATGATTGGCATCCTTGGTCGTGCCAGGCAGTTCGGCACCATCAATAAATTGCTTAATCAGATGGTGAATGAAGGATGTCAACCAAATGTTGTGACATACAATCGTCTGATTCATAGTTATGGTCGCGCAAACTATTTGAAAGACGCAATGAATGTGTTCAGTCAAATGCAAGAAACAGGTTGTGAGCCTGACCGTGTCACCTACTGTACACTCATTGACATCCATGCTAAATCTGGGTTTCTTGATGTCGCCCTTGGACTGTATGATAGGATGCAAGAGGCTGGTCTTTCGCCTGACACATTTACTTACAGTGTTATGATCAACTGCCTTGGGAAAGCTGGCCATTTAGCTGCAGCTCACCGGCTGTTCTGTGAGATGGTGGATCATGGTTGTGTTCCCAACCTGGTCACCTATAATATCATGATAGCTTTGCAGGCCAAAGCAAGGAACTATGAGACTGCTTTAAAGCTCTACCGTGACATGCAGGGTGCTGGATTTCAGCCAGATAAAGTGACTTATAGCATAGTAATGGAGGTGCTTGGCCATTGTGGATACCTTGAGGAGGCAGAAGCACTTTTTGGTGAAATGAAACGGAAGAATTGGGTACCTGATGAACCTGTTTATGGCCTTTTAGTTGATTTGTGGGGCAAGGCTGGTAATGTGCAAAAAGCCTGGGATTGGTATCAAGCAATGCTCCATGCTGGTTTGCGTCCTAATGTGCCTACTTGCAATTCTCTTCTCAGTGCGTTTCTTAGGGTGCACCGTTTGTCTGATGCCTATAACTTGTTGCAGAGCATGGTGGGCTTAGGTCTGAACCCTTCTTTGCAGACATATACCTTGCTTCTCAGTTGTTGCACAGAAGCACGATCACCATATGACATGGAATTTTGTTGTGAGCTCATGGCAGTCACACGCCATCCTGCTCATACATTCCTACTGTCTATGCCATCAGCAGGACCCGATGGCCAAAACGTGAGAGAACACATGAGCAGCTTCTTGGATCTGATGCATAGTGAGGATAGAGAAAGCAAGAGGGGGCTTGTAGATGCCGTAGTGGATTTCCTTCATAAATCAGGGCTCAAGGAGGAGGCTGGTTCTGTCTGGGAGGTGGCTGCGCAGAAGAATGTTTATCCAGACGCCGTCAAAGAGAAAGGCTCATGTTATTGGCTTATAAACCTACATGTTATGTCTGATGGTACTGCTGTGACAGCACTATCAAGGACACTTGCTTGGTTTCGCCAAGAAATGCTAATATCCGGTGTTTGTCCAAACCGAATTGATATTGTGACAGGATGGGGACGGCGGAGTAGGGTTACAGGAACTTCTATGGTGAGGCATGCAGTGCAGGAATTGCTGCACATGTTTAGCTTCCCGTTTTTCACCGAGAATGGGAATTCTGGGTGCTTTGTAGGATGTGGGGAGTCTCTTAATAGATGGTTACTCGAATCTTATGTGGAGCGGATGCATTTATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

879

Amino Acids

96.82

Weight (kDa)

8.72

Isoelectric Point (pI)

38.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	377 - 429	3.7e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	379 - 467	6.4e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	384 - 422	1.2e-08	PPR repeat family
PPR_3	PF13812	404 - 462	1.1e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	413 - 445	1.1e-10	PPR repeat
PPR_2	PF13041	416 - 464	8.1e-16	PPR repeat family
PPR	PF01535	419 - 449	3.8e-06	PPR repeat
PPR_3	PF13812	440 - 500	2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	448 - 480	1.3e-06	PPR repeat
PPR_2	PF13041	454 - 500	3.4e-11	PPR repeat family
PPR	PF01535	454 - 484	8.7e-06	PPR repeat
PPR_long	PF17177	469 - 572	2e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	476 - 532	4e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	483 - 515	1.5e-08	PPR repeat
PPR	PF01535	489 - 519	1.7e-06	PPR repeat
PPR_2	PF13041	494 - 530	1.3e-10	PPR repeat family
PPR_3	PF13812	509 - 569	1.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	521 - 568	3.5e-13	PPR repeat family
PPR	PF01535	559 - 588	4.6e-06	PPR repeat
PPR_3	PF13812	619 - 673	8.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	626 - 673	5e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005339)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18900 AT1G18900 AT1G18900 AT1G18900
fragaria_vesca	FvH4_5g15730 FvH4_5g15730 FvH4_5g15730
malus_domestica	MD06G1185900.v1.1 MD14G1192100.v1.1
prunus_persica	Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1
pyrus_communis	pycom06g16530 pycom14g15980
rosa_chinensis	RchiOBHm_Chr7g0180381
rosa_laevigata	RLG00000005272
rosa_multiflora	Rmu_sc0003526.1_g000009
rosa_roxburghii	Rroxscaffold_3G00272940
rosa_rugosa	Rorug06G0432600 Rorug06G0432700 Rorug06G0432800 Rorug06G0432800
rosa_samantha	Rh7AG033200 Rh7BG033100 Rh7CG035000 Rh7DG033600
rosa_wichuraiana	Rw7G002770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	462, 2336
Acc16I	TGCGCA	2 cut(s)	945, 2280
Acc36I	ACCTGC	1 cut(s)	898
Acc65I	GGTACC	1 cut(s)	1768
AccB1I	GGYRCC	2 cut(s)	1199, 1768
AccBSI	CCGCTC	1 cut(s)	2623
AccII	CGCG	2 cut(s)	21, 1290
AciI	CCGC	4 cut(s)	72, 734, 2467, 2623
AclI	AACGTT	1 cut(s)	357
AclWI	GGATC	3 cut(s)	202, 1554, 2172
AcoI	YGGCCR	3 cut(s)	1504, 1705, 2128
AcsI	RAATTY	6 cut(s)	10, 100, 1065, 1077, 2048, 2557
AcuI	CTGAAG	2 cut(s)	138, 612
AcyI	GRCGYC	1 cut(s)	2301
AfaI	GTAC	6 cut(s)	337, 637, 837, 1372, 1770, 2362
AfiI	CCNNNNNNNGG	7 cut(s)	141, 1608, 1726, 1787, 1842, 2117, 2553
AflIII	ACRYGT	2 cut(s)	2344, 2523
AgsI	TTSAA	7 cut(s)	883, 931, 965, 1041, 1072, 1082, 1303
AhlI	ACTAGT	1 cut(s)	587
AjnI	CCWGG	3 cut(s)	1187, 1566, 1835
AjuI	GAANNNNNNNTTGG	2 cut(s)	1725, 1757
AleI	CACNNNNGTG	1 cut(s)	590
Alw21I	GWGCWC	2 cut(s)	1926, 2064
Alw26I	GTCTC	5 cut(s)	541, 848, 1034, 1613, 2592
Alw44I	GTGCAC	1 cut(s)	1922
AlwI	GGATC	3 cut(s)	202, 1554, 2172
AlwNI	CAGNNNCTG	1 cut(s)	338
AoxI	GGCC	7 cut(s)	868, 1000, 1504, 1599, 1705, 1789, 2128
ApaI	GGGCCC	1 cut(s)	1004
ApaLI	GTGCAC	1 cut(s)	1922
ApoI	RAATTY	6 cut(s)	10, 100, 1065, 1077, 2048, 2557
Asp700I	GAANNNNTTC	1 cut(s)	1319
Asp718I	GGTACC	1 cut(s)	1768
AspLEI	GCGC	5 cut(s)	21, 23, 946, 1292, 2281
AspS9I	GGNCC	4 cut(s)	869, 1000, 1001, 2120
AsuHPI	GGTGA	9 cut(s)	381, 1240, 1354, 1514, 1564, 1757, 2025, 2506, 2536
AsuNHI	GCTAGC	1 cut(s)	350
AvaII	GGWCC	1 cut(s)	2120
BaeGI	GKGCMC	4 cut(s)	136, 1004, 1152, 1926
BaeI	ACNNNNGTAYC	2 cut(s)	2352, 2385
BalI	TGGCCA	3 cut(s)	1506, 1707, 2130
BanI	GGYRCC	2 cut(s)	1199, 1768
BanII	GRGCYC	3 cut(s)	1004, 2064, 2244
BbsI	GAAGAC	1 cut(s)	158
Bbv12I	GWGCWC	2 cut(s)	1926, 2064
BceAI	ACGGC	3 cut(s)	2198, 2288, 2480
BciT130I	CCWGG	3 cut(s)	1189, 1568, 1837
BciVI	GTATCC	1 cut(s)	1709
BclI	TGATCA	1 cut(s)	1482
BcoDI	GTCTC	5 cut(s)	541, 848, 1034, 1613, 2592
BcuI	ACTAGT	1 cut(s)	587
BfaI	CTAG	4 cut(s)	54, 351, 588, 861
BfmI	CTRYAG	3 cut(s)	399, 1008, 1515
BfuAI	ACCTGC	1 cut(s)	898
BfuI	GTATCC	1 cut(s)	1709
BlpI	GCTNAGC	1 cut(s)	798
Bme1390I	CCNGG	3 cut(s)	1189, 1568, 1837
Bme18I	GGWCC	1 cut(s)	2120
BmgT120I	GGNCC	4 cut(s)	869, 1000, 1001, 2120
BmiI	GGNNCC	5 cut(s)	1001, 1002, 1201, 1770, 2122
BmrFI	CCNGG	3 cut(s)	1189, 1568, 1837
BmsI	GCATC	9 cut(s)	226, 480, 604, 1182, 1425, 1927, 2160, 2200, 2616
BmtI	GCTAGC	1 cut(s)	354
BpiI	GAAGAC	1 cut(s)	158
BplI	GAGNNNNNCTC	2 cut(s)	2303, 2335
BpmI	CTGGAG	2 cut(s)	523, 738
Bpu10I	CCTNAGC	1 cut(s)	1969
Bpu1102I	GCTNAGC	1 cut(s)	798
BpuEI	CTTGAG	3 cut(s)	28, 1742, 2228
BsaBI	GATNNNNATC	2 cut(s)	1172, 1845
BsaHI	GRCGYC	1 cut(s)	2301
BsaJI	CCNNGG	5 cut(s)	528, 1177, 1417, 1492, 1836
BsaWI	WCCGGW	2 cut(s)	191, 2420
BsaXI	ACNNNNNCTCC	2 cut(s)	225, 255
Bsc4I	CCNNNNNNNGG	7 cut(s)	141, 1608, 1726, 1787, 1842, 2117, 2553
Bse118I	RCCGGY	1 cut(s)	1524
Bse1I	ACTGG	2 cut(s)	540, 678
Bse3DI	GCAATG	2 cut(s)	1317, 1860
Bse8I	GATNNNNATC	2 cut(s)	1172, 1845
BseBI	CCWGG	3 cut(s)	1189, 1568, 1837
BseDI	CCNNGG	5 cut(s)	528, 1177, 1417, 1492, 1836
BseJI	GATNNNNATC	2 cut(s)	1172, 1845
BseLI	CCNNNNNNNGG	7 cut(s)	141, 1608, 1726, 1787, 1842, 2117, 2553
BseMI	GCAATG	2 cut(s)	1317, 1860
BseMII	CTCAG	7 cut(s)	82, 258, 603, 741, 812, 1918, 2023
BseNI	ACTGG	2 cut(s)	540, 678
BsePI	GCGCGC	1 cut(s)	19
BseRI	GAGGAG	2 cut(s)	1739, 2264
BseSI	GKGCMC	4 cut(s)	136, 1004, 1152, 1926
BsgI	GTGCAG	2 cut(s)	2504, 2528
Bsh1236I	CGCG	2 cut(s)	21, 1290
BshFI	GGCC	7 cut(s)	870, 1002, 1506, 1601, 1707, 1791, 2130
BshNI	GGYRCC	2 cut(s)	1199, 1768
BsiHKAI	GWGCWC	2 cut(s)	1926, 2064
BsiSI	CCGG	3 cut(s)	192, 1525, 2421
BsiWI	CGTACG	1 cut(s)	635
BslFI	GGGAC	1 cut(s)	2475
BslI	CCNNNNNNNGG	7 cut(s)	141, 1608, 1726, 1787, 1842, 2117, 2553
BsmAI	GTCTC	5 cut(s)	541, 848, 1034, 1613, 2592
BsmFI	GGGAC	1 cut(s)	2475
BsmI	GAATGC	1 cut(s)	366
BsnI	GGCC	7 cut(s)	870, 1002, 1506, 1601, 1707, 1791, 2130
Bsp120I	GGGCCC	1 cut(s)	1000
Bsp1286I	GDGCHC	6 cut(s)	136, 1004, 1152, 1926, 2064, 2244
Bsp1407I	TGTACA	2 cut(s)	835, 1370
Bsp143I	GATC	7 cut(s)	194, 329, 1084, 1482, 1546, 2030, 2164
Bsp1720I	GCTNAGC	1 cut(s)	798
BspACI	CCGC	4 cut(s)	72, 734, 2467, 2623
BspANI	GGCC	7 cut(s)	870, 1002, 1506, 1601, 1707, 1791, 2130
BspCNI	CTCAG	7 cut(s)	81, 259, 604, 740, 811, 1917, 2022
BspFNI	CGCG	2 cut(s)	21, 1290
BspHI	TCATGA	2 cut(s)	763, 1584
BspLI	GGNNCC	5 cut(s)	1001, 1002, 1201, 1770, 2122
BspMAI	CTGCAG	2 cut(s)	1012, 1519
BspMI	ACCTGC	1 cut(s)	898
BspOI	GCTAGC	1 cut(s)	354
BspPI	GGATC	3 cut(s)	202, 1554, 2172
BspT107I	GGYRCC	2 cut(s)	1199, 1768
BsrBI	CCGCTC	1 cut(s)	2623
BsrDI	GCAATG	2 cut(s)	1317, 1860
BsrFI	RCCGGY	1 cut(s)	1524
BsrGI	TGTACA	2 cut(s)	835, 1370
BsrI	ACTGG	2 cut(s)	540, 678
BssAI	RCCGGY	1 cut(s)	1524
BssECI	CCNNGG	5 cut(s)	528, 1177, 1417, 1492, 1836
BssHII	GCGCGC	1 cut(s)	19
BssMI	GATC	7 cut(s)	194, 329, 1084, 1482, 1546, 2030, 2164
BssNI	GRCGYC	1 cut(s)	2301
BssT1I	CCWWGG	3 cut(s)	1177, 1417, 1492
Bst2UI	CCWGG	3 cut(s)	1189, 1568, 1837
Bst6I	CTCTTC	1 cut(s)	1905
BstACI	GRCGYC	1 cut(s)	2301
BstAPI	GCANNNNNTGC	2 cut(s)	2506, 2515
BstAUI	TGTACA	2 cut(s)	835, 1370
BstC8I	GCNNGC	6 cut(s)	21, 150, 352, 1504, 1599, 2502
BstDSI	CCRYGG	1 cut(s)	528
BstEII	GGTNACC	2 cut(s)	35, 1570
BstENI	CCTNNNNNAGG	1 cut(s)	1724
BstFNI	CGCG	2 cut(s)	21, 1290
BstHHI	GCGC	5 cut(s)	21, 23, 946, 1292, 2281
BstKTI	GATC	7 cut(s)	197, 332, 1087, 1485, 1549, 2033, 2167
BstMAI	GTCTC	5 cut(s)	541, 848, 1034, 1613, 2592
BstMBI	GATC	7 cut(s)	194, 329, 1084, 1482, 1546, 2030, 2164
BstNI	CCWGG	3 cut(s)	1189, 1568, 1837
BstNSI	RCATGY	4 cut(s)	1648, 2348, 2504, 2527
BstPI	GGTNACC	2 cut(s)	35, 1570
BstSCI	CCNGG	3 cut(s)	1187, 1566, 1835
BstSFI	CTRYAG	3 cut(s)	399, 1008, 1515
BstSLI	GKGCMC	4 cut(s)	136, 1004, 1152, 1926
BstUI	CGCG	2 cut(s)	21, 1290
BstV2I	GAAGAC	1 cut(s)	158
BstX2I	RGATCY	2 cut(s)	194, 2164
BstYI	RGATCY	2 cut(s)	194, 2164
BsuI	GTATCC	1 cut(s)	1709
BsuRI	GGCC	7 cut(s)	870, 1002, 1506, 1601, 1707, 1791, 2130
BtgI	CCRYGG	1 cut(s)	528
BtsI	GCAGTG	3 cut(s)	246, 944, 2511
BtsIMutI	CAGTG	8 cut(s)	246, 510, 547, 748, 944, 1480, 1912, 2511
BveI	ACCTGC	1 cut(s)	898
Cac8I	GCNNGC	6 cut(s)	21, 150, 352, 1504, 1599, 2502
CaiI	CAGNNNCTG	1 cut(s)	338
CciI	TCATGA	2 cut(s)	763, 1584
CfoI	GCGC	5 cut(s)	21, 23, 946, 1292, 2281
Cfr10I	RCCGGY	1 cut(s)	1524
Cfr13I	GGNCC	4 cut(s)	869, 1000, 1001, 2120
CseI	GACGC	4 cut(s)	1055, 1316, 1862, 2309
CsiI	ACCWGGT	1 cut(s)	1566
Csp6I	GTAC	6 cut(s)	336, 636, 836, 1371, 1769, 2361
CspCI	CAANNNNNGTGG	2 cut(s)	1150, 1185
CviQI	GTAC	6 cut(s)	336, 636, 836, 1371, 1769, 2361
DpnI	GATC	7 cut(s)	196, 331, 1086, 1484, 1548, 2032, 2166
DpnII	GATC	7 cut(s)	194, 329, 1084, 1482, 1546, 2030, 2164
DraI	TTTAAA	1 cut(s)	1629
EaeI	YGGCCR	3 cut(s)	1504, 1705, 2128
Eam1104I	CTCTTC	1 cut(s)	1905
EarI	CTCTTC	1 cut(s)	1905
EciI	GGCGGA	1 cut(s)	2482
Ecl136II	GAGCTC	1 cut(s)	2062
Eco130I	CCWWGG	3 cut(s)	1177, 1417, 1492
Eco24I	GRGCYC	3 cut(s)	1004, 2064, 2244
Eco47I	GGWCC	1 cut(s)	2120
Eco53kI	GAGCTC	1 cut(s)	2062
Eco57I	CTGAAG	2 cut(s)	138, 612
Eco91I	GGTNACC	2 cut(s)	35, 1570
EcoICRI	GAGCTC	1 cut(s)	2062
EcoNI	CCTNNNNNAGG	1 cut(s)	1724
EcoO109I	RGGNCCY	3 cut(s)	1000, 1001, 2120
EcoO65I	GGTNACC	2 cut(s)	35, 1570
EcoRI	GAATTC	1 cut(s)	2557
EcoRII	CCWGG	3 cut(s)	1187, 1566, 1835
EcoT14I	CCWWGG	3 cut(s)	1177, 1417, 1492
EcoT22I	ATGCAT	2 cut(s)	2175, 2631
EcoT38I	GRGCYC	3 cut(s)	1004, 2064, 2244
ErhI	CCWWGG	3 cut(s)	1177, 1417, 1492
FaqI	GGGAC	1 cut(s)	2475
FauI	CCCGC	1 cut(s)	65
FauNDI	CATATG	1 cut(s)	2038
FbaI	TGATCA	1 cut(s)	1482
FriOI	GRGCYC	3 cut(s)	1004, 2064, 2244
FspAI	RTGCGCAY	1 cut(s)	945
FspBI	CTAG	4 cut(s)	54, 351, 588, 861
FspI	TGCGCA	2 cut(s)	945, 2280
GlaI	GCGC	5 cut(s)	20, 22, 945, 1291, 2280
GsuI	CTGGAG	2 cut(s)	523, 738
HaeIII	GGCC	7 cut(s)	870, 1002, 1506, 1601, 1707, 1791, 2130
HapII	CCGG	3 cut(s)	192, 1525, 2421
HgaI	GACGC	4 cut(s)	1055, 1316, 1862, 2309
HhaI	GCGC	5 cut(s)	21, 23, 946, 1292, 2281
Hin1I	GRCGYC	1 cut(s)	2301
Hin6I	GCGC	5 cut(s)	19, 21, 944, 1290, 2279
HinP1I	GCGC	5 cut(s)	19, 21, 944, 1290, 2279
HincII	GTYRAC	2 cut(s)	277, 1244
HindII	GTYRAC	2 cut(s)	277, 1244
HinfI	GANTC	9 cut(s)	219, 254, 466, 534, 751, 1134, 1273, 2585, 2609
HpaII	CCGG	3 cut(s)	192, 1525, 2421
HphI	GGTGA	9 cut(s)	381, 1240, 1354, 1514, 1564, 1757, 2025, 2506, 2536
Hpy166II	GTNNAC	4 cut(s)	277, 1244, 1373, 1924
Hpy188III	TCNNGA	9 cut(s)	78, 231, 755, 764, 818, 1082, 1406, 1585, 2297
Hpy8I	GTNNAC	4 cut(s)	277, 1244, 1373, 1924
HpyAV	CCTTC	7 cut(s)	213, 367, 765, 1014, 1229, 1992, 2237
HpyCH4IV	ACGT	2 cut(s)	357, 2136
HpySE526I	ACGT	2 cut(s)	357, 2136
Hsp92I	GRCGYC	1 cut(s)	2301
HspAI	GCGC	5 cut(s)	19, 21, 944, 1290, 2279
KpnI	GGTACC	1 cut(s)	1772
Ksp22I	TGATCA	1 cut(s)	1482
Kzo9I	GATC	7 cut(s)	194, 329, 1084, 1482, 1546, 2030, 2164
LmnI	GCTCC	3 cut(s)	928, 1863, 2620
LweI	GCATC	9 cut(s)	226, 480, 604, 1182, 1425, 1927, 2160, 2200, 2616
MabI	ACCWGGT	1 cut(s)	1566
MaeI	CTAG	4 cut(s)	54, 351, 588, 861
MaeII	ACGT	2 cut(s)	357, 2136
MalI	GATC	7 cut(s)	196, 331, 1086, 1484, 1548, 2032, 2166
MbiI	CCGCTC	1 cut(s)	2623
MboI	GATC	7 cut(s)	194, 329, 1084, 1482, 1546, 2030, 2164
MboII	GAAGA	5 cut(s)	131, 158, 1771, 1892, 2296
MflI	RGATCY	2 cut(s)	194, 2164
MhlI	GDGCHC	6 cut(s)	136, 1004, 1152, 1926, 2064, 2244
MlsI	TGGCCA	3 cut(s)	1506, 1707, 2130
MluNI	TGGCCA	3 cut(s)	1506, 1707, 2130
MlyI	GAGTC	4 cut(s)	248, 543, 745, 2594
Mox20I	TGGCCA	3 cut(s)	1506, 1707, 2130
Mph1103I	ATGCAT	2 cut(s)	2175, 2631
MroXI	GAANNNNTTC	1 cut(s)	1319
MscI	TGGCCA	3 cut(s)	1506, 1707, 2130
MseI	TTAA	7 cut(s)	414, 993, 1110, 1118, 1218, 1628, 2592
MslI	CAYNNNNRTG	5 cut(s)	590, 1163, 1281, 1554, 1691
Msp20I	TGGCCA	3 cut(s)	1506, 1707, 2130
MspA1I	CMGCKG	3 cut(s)	72, 431, 734
MspI	CCGG	3 cut(s)	192, 1525, 2421
MspR9I	CCNGG	3 cut(s)	1189, 1568, 1837
Mva1269I	GAATGC	1 cut(s)	366
MvaI	CCWGG	3 cut(s)	1189, 1568, 1837
MvnI	CGCG	2 cut(s)	21, 1290
NdeI	CATATG	1 cut(s)	2038
NdeII	GATC	7 cut(s)	194, 329, 1084, 1482, 1546, 2030, 2164
NheI	GCTAGC	1 cut(s)	350
NlaIV	GGNNCC	5 cut(s)	1001, 1002, 1201, 1770, 2122
NmuCI	GTSAC	9 cut(s)	75, 1255, 1360, 1570, 1640, 1675, 2071, 2368, 2449
NsbI	TGCGCA	2 cut(s)	945, 2280
NsiI	ATGCAT	2 cut(s)	2175, 2631
NspI	RCATGY	4 cut(s)	1648, 2348, 2504, 2527
OliI	CACNNNNGTG	1 cut(s)	590
PaeI	GCATGC	1 cut(s)	2504
PagI	TCATGA	2 cut(s)	763, 1584
PauI	GCGCGC	1 cut(s)	19
PciI	ACATGT	2 cut(s)	2344, 2523
PctI	GAATGC	1 cut(s)	366
PdmI	GAANNNNTTC	1 cut(s)	1319
PfeI	GAWTC	5 cut(s)	219, 466, 1134, 1273, 2609
Pfl23II	CGTACG	1 cut(s)	635
PflFI	GACNNNGTC	2 cut(s)	1358, 2303
PleI	GAGTC	4 cut(s)	248, 542, 745, 2593
PpsI	GAGTC	4 cut(s)	248, 542, 745, 2593
PpuMI	RGGWCCY	1 cut(s)	2120
PscI	ACATGT	2 cut(s)	2344, 2523
PsiI	TTATAA	2 cut(s)	462, 2336
Psp124BI	GAGCTC	1 cut(s)	2064
Psp1406I	AACGTT	1 cut(s)	357
Psp5II	RGGWCCY	1 cut(s)	2120
Psp6I	CCWGG	3 cut(s)	1187, 1566, 1835
PspEI	GGTNACC	2 cut(s)	35, 1570
PspGI	CCWGG	3 cut(s)	1187, 1566, 1835
PspLI	CGTACG	1 cut(s)	635
PspN4I	GGNNCC	5 cut(s)	1001, 1002, 1201, 1770, 2122
PspOMI	GGGCCC	1 cut(s)	1000
PspPI	GGNCC	4 cut(s)	869, 1000, 1001, 2120
PspPPI	RGGWCCY	1 cut(s)	2120
PstI	CTGCAG	2 cut(s)	1012, 1519
PstNI	CAGNNNCTG	1 cut(s)	338
PsuI	RGATCY	2 cut(s)	194, 2164
PsyI	GACNNNGTC	2 cut(s)	1358, 2303
PteI	GCGCGC	1 cut(s)	19
PvuII	CAGCTG	1 cut(s)	431
RsaI	GTAC	6 cut(s)	337, 637, 837, 1372, 1770, 2362
RsaNI	GTAC	6 cut(s)	336, 636, 836, 1371, 1769, 2361
RseI	CAYNNNNRTG	5 cut(s)	590, 1163, 1281, 1554, 1691
SacI	GAGCTC	1 cut(s)	2064
SaqAI	TTAA	7 cut(s)	414, 993, 1110, 1118, 1218, 1628, 2592
Sau3AI	GATC	7 cut(s)	194, 329, 1084, 1482, 1546, 2030, 2164
Sau96I	GGNCC	4 cut(s)	869, 1000, 1001, 2120
SchI	GAGTC	4 cut(s)	248, 543, 745, 2594
ScrFI	CCNGG	3 cut(s)	1189, 1568, 1837
SduI	GDGCHC	6 cut(s)	136, 1004, 1152, 1926, 2064, 2244
SexAI	ACCWGGT	1 cut(s)	1566
SfaNI	GCATC	9 cut(s)	226, 480, 604, 1182, 1425, 1927, 2160, 2200, 2616
SfcI	CTRYAG	3 cut(s)	399, 1008, 1515
SinI	GGWCC	1 cut(s)	2120
SmiMI	CAYNNNNRTG	5 cut(s)	590, 1163, 1281, 1554, 1691
SmlI	CTYRAG	3 cut(s)	43, 1721, 2243
SmoI	CTYRAG	3 cut(s)	43, 1721, 2243
SpeI	ACTAGT	1 cut(s)	587
SphI	GCATGC	1 cut(s)	2504
SsiI	CCGC	4 cut(s)	72, 734, 2467, 2623
SspMI	CTAG	4 cut(s)	54, 351, 588, 861
SstI	GAGCTC	1 cut(s)	2064
StyD4I	CCNGG	3 cut(s)	1187, 1566, 1835
StyI	CCWWGG	3 cut(s)	1177, 1417, 1492
TaiI	ACGT	2 cut(s)	360, 2139
TaqI	TCGA	1 cut(s)	2607
TatI	WGTACW	2 cut(s)	835, 1370
TauI	GCSGC	1 cut(s)	737
TfiI	GAWTC	5 cut(s)	219, 466, 1134, 1273, 2609
Tru1I	TTAA	7 cut(s)	414, 993, 1110, 1118, 1218, 1628, 2592
Tru9I	TTAA	7 cut(s)	414, 993, 1110, 1118, 1218, 1628, 2592
TscAI	CASTG	8 cut(s)	246, 517, 547, 748, 944, 1480, 1912, 2511
TseFI	GTSAC	9 cut(s)	75, 1255, 1360, 1570, 1640, 1675, 2071, 2368, 2449
Tsp45I	GTSAC	9 cut(s)	75, 1255, 1360, 1570, 1640, 1675, 2071, 2368, 2449
TspDTI	ATGAA	9 cut(s)	93, 138, 917, 1248, 1265, 1328, 1766, 1791, 2219
TspGWI	ACGGA	1 cut(s)	1771
TspRI	CASTG	8 cut(s)	246, 517, 547, 748, 944, 1480, 1912, 2511
Tth111I	GACNNNGTC	2 cut(s)	1358, 2303
VneI	GTGCAC	1 cut(s)	1922
VpaK11BI	GGWCC	1 cut(s)	2120
XagI	CCTNNNNNAGG	1 cut(s)	1724
XapI	RAATTY	6 cut(s)	10, 100, 1065, 1077, 2048, 2557
XceI	RCATGY	4 cut(s)	1648, 2348, 2504, 2527
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1394
XmnI	GAANNNNTTC	1 cut(s)	1319
XspI	CTAG	4 cut(s)	54, 351, 588, 861
Zsp2I	ATGCAT	2 cut(s)	2175, 2631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.