Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0005339

Physical Location & Seq

Genomic Coordinates Reverse (-)

32372121 .. 32376291

Gene Structure 4171 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1185900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2625 bp

ATGTTGCGAGCAAAGCAATTAAGTAACCTTTCAAGCAGTGCGAGGTCATTCTTTCTTACCGGGCCTGGGCCACGATGTAGTGCGACAGATGGAAATTCATGCTCTTGCTCTGAAGATGAAACTTGTGTTTCGCAAAGGCAGCTAGCAAGAGATGGAGGTCTACTTGCCCAAAATCCATCCACCATGGTATCCAAACCTTCAGCAAGGGCAAGGGCAGGAACCATCCTTTTGGGAGATGCAGTAAAAGTGGCAGGTCCTCGTAAAGCTGGAGGTGTTGACCATACAGCTTCTATTAATCAGGCTGCAACAGCACCAAGATCTTTTGGGAGATCGGAGACTGTAAGTTATGCTAGTGGCACAGATGTGGTGCACTCATCACCTCTTGTTGCAGACCAGTTTGCTAAGGCTGGAGTTGCAGCTGTAAATTTTCTGTCTGATATAGTAAATGGCAAGCTTCCTTTATCTGATGGGCTTGGAATCCTTAATAACTGTATGGTTGATCCAACCAGGCCCCTCAACGGTATCAAGCCATCACATGTGAAACAAATAAAAAGAGAACATTTCACTAATGGTCGTCCAAAACCAGCTACTGAGGTATCCGCTGCATCAAAGCCTACAAGTAATTATCATGGTCCAAAGGGCAAAGGTGAGAAATCCAATTTCGTTAAAGGTCTAAATCATGTTCCATATACTAGGACGGAAAGCTCAGCAACACCTCATACTGTATACTTGGACCATCATGAGCAAAGGTCTATGCCACCGAAATCAAAACCACATTCAAACTTCATTCCAAATTACAGTTCCAGTGCTCAGACCTCTGATGCAGAGACCATGGGTCATATGACTCGTGCCACTAAAAGTTTCAACAGGCCCATAAGAGATGTGAAAATGCCAACAGGAATAGCTCCCCAAGTCAACAGACAGTTTGCTCACACTGGGAATGTTGTTCATAATGTTTCTCACATACTGCAACAGACACGATGGGGTCCTGCTGCAGAGGCCACTCTTGAAAATCTCAACTGTTCAATGGATGCATATCAAGCAAACCAAATTTTAAAGCAACTTCAAGATCATTCCGTTGCTCTTGGATTTTTTTATTGGCTTAAGCGGCAAGCAGGATTCAAGCATGATGGGCACACTTATACCACCATGGTTGGGATCCTTGGTCGTTCCAGGCAGTTTGGTGCCATTAACAAGTTGCTTAATCAGATGGTGAAAGAAGGATGCCGGCCAAATGTTGTGACATATAACCGTCTGATTCATAGTTATGGCCGTGCTAACTATTTGAAAGAAGCAATGAATGTTTTCAATCAAATGCAAGAAGCGGGATGTGAACCTGACCGTGTCACCTACTCCACACTAATTGACATCCATGCAAAAGCTGGATTTCTTGACGCTGCCCTAGGACTATATGACAGTATGCAAGAGGCTGGTCTTGCTCCCGACACATTCACTTACAGTGTTATGATCAACTGTCTTGGGAAAGCTGGTCATTTAGATGCTGCTCATCGGCTATTTTGTGAGATGGTTAATCATGGTTGTGTTCCCAATTTGGTCACCTACAATATCATGATAGCTTTGCAGGCCAAGGCAAGGAATTATGAGACTTCTTTAAAGCTCTACCGTGACATGCAGGGTGCGGGATTTGAACCGGATAAAGTAACTTATAGCATAGTAATGGAGGTCCTTGGCCACTGTGGATATCTTGAGGAAGCCGAGGTGATTTTTCAAGAAATGAAACAGAAAAACTGGGTGCCTGATGAGCCTGTTTATGGTCTTTTGGTAGATTTGTGGGGCAAGGCTGGTAACATAGAAAAAGCCTGGAGTTGGTATCAAGCTATGCTCGATGCAGGTTTACGCCCTAATGTGCCTACTTGCAATTCTCTGCTCAGTGCGTTTCTTAGGGTGCACCAGCTGTCCGATGCCTATAACTTGCTGCAGAGCATGATGGGTTTAGGTCTAAACCCTTCTTTGCAGACATATACATTGCTTCTCAGTTGTTGCACAGAAGCACAATCACCATATGACATGGGATTTTGTGGTGACCTTATGGCAGTCACAGGCCATCCTGCACATACATTCTTACTGTCTATGCCATCGGCAGGACCTGATGGTCAAAACGTGCGGGAGCACATGAGCCGGTTCTTGGATATGATGCATAGCGAGGATAGAGAAAGCAAGAGGGGCCTTGTAGATGCAGTAGTCGACTTTCTTCACAAGGCGGGACTAAAGGAGGAGGCGGGTTCAGTCTGGGAGGTGGCTGCACAAAAGAATGTCTATCCGGACGCCATCAAAGAGAAAAGCTCTTGTTATTGGCTTATAAACCTGCACGTTATGTCCGACGGTACTGCTGTGATAGCTCTATCAAGAACACTCGCATGGTTTCGCCAACAGATGCTAATGTCCGGGATTTGTCCAAGCCGGATTGATATTGTGACAGGATGGGGCCGTCGGAGTAGGGTTACAGGATCTTCTTTGGTTAGGCAGGCAGTGCAGGAACTGCTGAATGTTTTTCGCTTCCCGTTCTTCACTGAAAACGGCAATTCTGGTTGTTTTGTGGGATGTGGGGAGCCTCTTAACAGGTGGTTGCTCCAGTCTTACGTGGAGCGAATGCATTTATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

875

Amino Acids

96.19

Weight (kDa)

8.65

Isoelectric Point (pI)

39.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	372 - 462	4.5e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	372 - 424	2.3e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	379 - 417	1.9e-08	PPR repeat family
PPR_1	PF12854	408 - 440	5.4e-11	PPR repeat
PPR_2	PF13041	411 - 459	2.7e-17	PPR repeat family
PPR_3	PF13812	413 - 457	9.7e-09	Pentatricopeptide repeat domain
PPR	PF01535	414 - 444	9.1e-08	PPR repeat
PPR_1	PF12854	443 - 474	9.3e-08	PPR repeat
PPR	PF01535	449 - 479	2.7e-06	PPR repeat
PPR_2	PF13041	449 - 495	3.2e-12	PPR repeat family
TPR_24	PF23276	449 - 544	6.1e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	464 - 565	1.8e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	471 - 526	1.5e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	478 - 510	3.4e-09	PPR repeat
PPR	PF01535	484 - 514	1.8e-07	PPR repeat
PPR_2	PF13041	489 - 525	1.5e-11	PPR repeat family
PPR_2	PF13041	516 - 563	1.1e-11	PPR repeat family
PPR_long	PF17177	572 - 671	1.3e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	614 - 668	1.8e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	621 - 668	7.7e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005339)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18900 AT1G18900 AT1G18900 AT1G18900
fragaria_vesca	FvH4_5g15730 FvH4_5g15730 FvH4_5g15730
malus_domestica	MD06G1185900.v1.1 MD14G1192100.v1.1
prunus_persica	Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1
pyrus_communis	pycom06g16530 pycom14g15980
rosa_chinensis	RchiOBHm_Chr7g0180381
rosa_laevigata	RLG00000005272
rosa_multiflora	Rmu_sc0003526.1_g000009
rosa_roxburghii	Rroxscaffold_3G00272940
rosa_rugosa	Rorug06G0432600 Rorug06G0432700 Rorug06G0432800 Rorug06G0432800
rosa_samantha	Rh7AG033200 Rh7BG033100 Rh7CG035000 Rh7DG033600
rosa_wichuraiana	Rw7G002770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2321
AasI	GACNNNNNNGTC	1 cut(s)	2112
Acc36I	ACCTGC	3 cut(s)	242, 1841, 2334
AccB1I	GGYRCC	2 cut(s)	1184, 1753
AccI	GTMKAC	3 cut(s)	160, 726, 2205
AccIII	TCCGGA	1 cut(s)	2281
AciI	CCGC	7 cut(s)	600, 1108, 1325, 1640, 2125, 2222, 2240
AclWI	GGATC	4 cut(s)	494, 1153, 1166, 2476
AcoI	YGGCCR	3 cut(s)	1229, 1270, 1690
AcsI	RAATTY	3 cut(s)	94, 424, 1050
AcuI	CTGAAG	2 cut(s)	132, 183
AcyI	GRCGYC	1 cut(s)	2286
AfaI	GTAC	1 cut(s)	2347
AfiI	CCNNNNNNNGG	8 cut(s)	66, 518, 1155, 1593, 1772, 1827, 2102, 2146
AflII	CTTAAG	1 cut(s)	1103
AflIII	ACRYGT	1 cut(s)	535
AhdI	GACNNNNNGTC	1 cut(s)	834
AjnI	CCWGG	4 cut(s)	64, 506, 1172, 1820
AjuI	GAANNNNNNNTTGG	2 cut(s)	784, 816
AleI	CACNNNNGTG	1 cut(s)	362
Alw21I	GWGCWC	4 cut(s)	372, 811, 1911, 2133
Alw26I	GTCTC	3 cut(s)	329, 821, 1598
Alw44I	GTGCAC	2 cut(s)	368, 1907
AlwI	GGATC	4 cut(s)	494, 1153, 1166, 2476
AlwNI	CAGNNNCTG	3 cut(s)	590, 2108, 2500
Aor13HI	TCCGGA	1 cut(s)	2281
ApaLI	GTGCAC	2 cut(s)	368, 1907
ApeKI	GCWGC	9 cut(s)	139, 302, 416, 602, 992, 1397, 1502, 1936, 2261
ApoI	RAATTY	3 cut(s)	94, 424, 1050
AseI	ATTAAT	1 cut(s)	294
Asp700I	GAANNNNTTC	1 cut(s)	1304
AspA2I	CCTAGG	1 cut(s)	1402
AsuC2I	CCSGG	2 cut(s)	61, 2407
AsuHPI	GGTGA	8 cut(s)	369, 659, 1225, 1339, 1549, 1732, 2010, 2054
AsuNHI	GCTAGC	1 cut(s)	142
AvaII	GGWCC	6 cut(s)	254, 632, 733, 986, 1684, 2105
AvrII	CCTAGG	1 cut(s)	1402
BaeGI	GKGCMC	3 cut(s)	372, 1137, 1911
BaeI	ACNNNNGTAYC	6 cut(s)	171, 204, 579, 612, 2337, 2370
BalI	TGGCCA	1 cut(s)	1692
BamHI	GGATCC	1 cut(s)	1158
BanI	GGYRCC	2 cut(s)	1184, 1753
BauI	CACGAG	1 cut(s)	846
Bbv12I	GWGCWC	4 cut(s)	372, 811, 1911, 2133
BbvI	GCAGC	9 cut(s)	151, 289, 428, 589, 979, 1384, 1489, 1923, 2248
BceAI	ACGGC	3 cut(s)	1257, 2433, 2554
BcgI	CGANNNNNNTGC	2 cut(s)	2330, 2364
BciT130I	CCWGG	4 cut(s)	66, 508, 1174, 1822
BciVI	GTATCC	2 cut(s)	199, 607
BclI	TGATCA	1 cut(s)	1467
BcnI	CCSGG	2 cut(s)	61, 2407
BcoDI	GTCTC	3 cut(s)	329, 821, 1598
BfaI	CTAG	4 cut(s)	143, 351, 693, 1403
BfmI	CTRYAG	2 cut(s)	993, 1937
BfrI	CTTAAG	1 cut(s)	1103
BfuAI	ACCTGC	3 cut(s)	242, 1841, 2334
BfuI	GTATCC	2 cut(s)	199, 607
BglII	AGATCT	1 cut(s)	317
BlnI	CCTAGG	1 cut(s)	1402
BlpI	GCTNAGC	1 cut(s)	706
Bme1390I	CCNGG	6 cut(s)	61, 66, 508, 1174, 1822, 2407
Bme18I	GGWCC	6 cut(s)	254, 632, 733, 986, 1684, 2105
BmeRI	GACNNNNNGTC	1 cut(s)	834
BmiI	GGNNCC	9 cut(s)	220, 512, 987, 1160, 1186, 1755, 2186, 2447, 2571
BmrFI	CCNGG	6 cut(s)	61, 66, 508, 1174, 1822, 2407
BmrI	ACTGGG	2 cut(s)	945, 1759
BmtI	GCTAGC	1 cut(s)	146
BmuI	ACTGGG	2 cut(s)	945, 1759
BplI	GAGNNNNNCTC	2 cut(s)	2288, 2320
BpmI	CTGGAG	4 cut(s)	288, 429, 1843, 2576
Bpu10I	CCTNAGC	1 cut(s)	402
Bpu1102I	GCTNAGC	1 cut(s)	706
BpuEI	CTTGAG	1 cut(s)	1727
BpuMI	CCSGG	2 cut(s)	61, 2407
BsaAI	YACGTR	1 cut(s)	2602
BsaBI	GATNNNNATC	1 cut(s)	1035
BsaHI	GRCGYC	1 cut(s)	2286
BsaI	GGTCTC	1 cut(s)	821
BsaJI	CCNNGG	9 cut(s)	65, 183, 831, 1149, 1162, 1402, 1587, 1687, 1716
BsaWI	WCCGGW	2 cut(s)	1651, 2281
BsaXI	ACNNNNNCTCC	2 cut(s)	225, 255
Bsc4I	CCNNNNNNNGG	8 cut(s)	66, 518, 1155, 1593, 1772, 1827, 2102, 2146
Bse118I	RCCGGY	2 cut(s)	1227, 2139
Bse1I	ACTGG	5 cut(s)	394, 804, 940, 1754, 2593
Bse3DI	GCAATG	2 cut(s)	1302, 1985
Bse8I	GATNNNNATC	1 cut(s)	1035
BseAI	TCCGGA	1 cut(s)	2281
BseBI	CCWGG	4 cut(s)	66, 508, 1174, 1822
BseDI	CCNNGG	9 cut(s)	65, 183, 831, 1149, 1162, 1402, 1587, 1687, 1716
BseGI	GGATG	9 cut(s)	176, 222, 1036, 1229, 1334, 1368, 2065, 2447, 2567
BseJI	GATNNNNATC	1 cut(s)	1035
BseLI	CCNNNNNNNGG	8 cut(s)	66, 518, 1155, 1593, 1772, 1827, 2102, 2146
BseMI	GCAATG	2 cut(s)	1302, 1985
BseMII	CTCAG	5 cut(s)	582, 720, 824, 1903, 2008
BseNI	ACTGG	5 cut(s)	394, 804, 940, 1754, 2593
BseRI	GAGGAG	1 cut(s)	2249
BseSI	GKGCMC	3 cut(s)	372, 1137, 1911
BseXI	GCAGC	9 cut(s)	151, 289, 428, 589, 979, 1384, 1489, 1923, 2248
BsgI	GTGCAG	4 cut(s)	2055, 2247, 2312, 2513
BshNI	GGYRCC	2 cut(s)	1184, 1753
BsiHKAI	GWGCWC	4 cut(s)	372, 811, 1911, 2133
BsiSI	CCGG	7 cut(s)	60, 1228, 1652, 2140, 2282, 2406, 2422
BslFI	GGGAC	1 cut(s)	2238
BslI	CCNNNNNNNGG	8 cut(s)	66, 518, 1155, 1593, 1772, 1827, 2102, 2146
BsmAI	GTCTC	3 cut(s)	329, 821, 1598
BsmFI	GGGAC	1 cut(s)	2238
BsmI	GAATGC	1 cut(s)	2616
Bso31I	GGTCTC	1 cut(s)	821
Bsp1286I	GDGCHC	5 cut(s)	372, 811, 1137, 1911, 2133
Bsp13I	TCCGGA	1 cut(s)	2281
Bsp143I	GATC	7 cut(s)	317, 329, 499, 1069, 1158, 1467, 2468
Bsp1720I	GCTNAGC	1 cut(s)	706
Bsp19I	CCATGG	3 cut(s)	183, 831, 1149
BspACI	CCGC	7 cut(s)	600, 1108, 1325, 1640, 2125, 2222, 2240
BspCNI	CTCAG	5 cut(s)	583, 719, 823, 1902, 2007
BspEI	TCCGGA	1 cut(s)	2281
BspHI	TCATGA	2 cut(s)	739, 1569
BspLI	GGNNCC	9 cut(s)	220, 512, 987, 1160, 1186, 1755, 2186, 2447, 2571
BspMAI	CTGCAG	2 cut(s)	997, 1941
BspMI	ACCTGC	3 cut(s)	242, 1841, 2334
BspOI	GCTAGC	1 cut(s)	146
BspPI	GGATC	4 cut(s)	494, 1153, 1166, 2476
BspT107I	GGYRCC	2 cut(s)	1184, 1753
BspTI	CTTAAG	1 cut(s)	1103
BspTNI	GGTCTC	1 cut(s)	821
BsrDI	GCAATG	2 cut(s)	1302, 1985
BsrFI	RCCGGY	2 cut(s)	1227, 2139
BsrI	ACTGG	5 cut(s)	394, 804, 940, 1754, 2593
BssAI	RCCGGY	2 cut(s)	1227, 2139
BssECI	CCNNGG	9 cut(s)	65, 183, 831, 1149, 1162, 1402, 1587, 1687, 1716
BssMI	GATC	7 cut(s)	317, 329, 499, 1069, 1158, 1467, 2468
BssNAI	GTATAC	1 cut(s)	727
BssNI	GRCGYC	1 cut(s)	2286
BssSI	CACGAG	1 cut(s)	846
BssT1I	CCWWGG	7 cut(s)	183, 831, 1149, 1162, 1402, 1587, 1687
Bst1107I	GTATAC	1 cut(s)	727
Bst2BI	CACGAG	1 cut(s)	846
Bst2UI	CCWGG	4 cut(s)	66, 508, 1174, 1822
BstACI	GRCGYC	1 cut(s)	2286
BstAFI	CTTAAG	1 cut(s)	1103
BstAPI	GCANNNNNTGC	2 cut(s)	2491, 2500
BstBAI	YACGTR	1 cut(s)	2602
BstC8I	GCNNGC	7 cut(s)	9, 144, 452, 1113, 1229, 1584, 2487
BstDEI	CTNAG	7 cut(s)	402, 591, 706, 810, 1889, 1901, 1994
BstDSI	CCRYGG	3 cut(s)	183, 831, 1149
BstEII	GGTNACC	2 cut(s)	1555, 2042
BstF5I	GGATG	9 cut(s)	176, 222, 1036, 1229, 1334, 1368, 2065, 2447, 2567
BstKTI	GATC	7 cut(s)	320, 332, 502, 1072, 1161, 1470, 2471
BstMAI	GTCTC	3 cut(s)	329, 821, 1598
BstMBI	GATC	7 cut(s)	317, 329, 499, 1069, 1158, 1467, 2468
BstNI	CCWGG	4 cut(s)	66, 508, 1174, 1822
BstNSI	RCATGY	2 cut(s)	539, 1633
BstPI	GGTNACC	2 cut(s)	1555, 2042
BstSCI	CCNGG	6 cut(s)	59, 64, 506, 1172, 1820, 2405
BstSFI	CTRYAG	2 cut(s)	993, 1937
BstSLI	GKGCMC	3 cut(s)	372, 1137, 1911
BstV1I	GCAGC	9 cut(s)	151, 289, 428, 589, 979, 1384, 1489, 1923, 2248
BstX2I	RGATCY	3 cut(s)	317, 1158, 2468
BstXI	CCANNNNNNTGG	1 cut(s)	229
BstYI	RGATCY	3 cut(s)	317, 1158, 2468
BstZ17I	GTATAC	1 cut(s)	727
BsuI	GTATCC	2 cut(s)	199, 607
BtgI	CCRYGG	3 cut(s)	183, 831, 1149
BtsCI	GGATG	9 cut(s)	176, 222, 1036, 1229, 1334, 1368, 2065, 2447, 2567
BtsI	GCAGTG	2 cut(s)	43, 2496
BtsIMutI	CAGTG	8 cut(s)	43, 811, 933, 1465, 1693, 1897, 2496, 2529
BveI	ACCTGC	3 cut(s)	242, 1841, 2334
Cac8I	GCNNGC	7 cut(s)	9, 144, 452, 1113, 1229, 1584, 2487
CaiI	CAGNNNCTG	3 cut(s)	590, 2108, 2500
CciI	TCATGA	2 cut(s)	739, 1569
Cfr10I	RCCGGY	2 cut(s)	1227, 2139
CseI	GACGC	2 cut(s)	1403, 2294
Csp6I	GTAC	1 cut(s)	2346
CspCI	CAANNNNNGTGG	4 cut(s)	1135, 1170, 1345, 1380
CviQI	GTAC	1 cut(s)	2346
DdeI	CTNAG	7 cut(s)	402, 591, 706, 810, 1889, 1901, 1994
DpnI	GATC	7 cut(s)	319, 331, 501, 1071, 1160, 1469, 2470
DpnII	GATC	7 cut(s)	317, 329, 499, 1069, 1158, 1467, 2468
DraI	TTTAAA	2 cut(s)	1056, 1614
DrdI	GACNNNNNNGTC	1 cut(s)	2112
DriI	GACNNNNNGTC	1 cut(s)	834
DseDI	GACNNNNNNGTC	1 cut(s)	2112
EaeI	YGGCCR	3 cut(s)	1229, 1270, 1690
Eam1105I	GACNNNNNGTC	1 cut(s)	834
Eco130I	CCWWGG	7 cut(s)	183, 831, 1149, 1162, 1402, 1587, 1687
Eco31I	GGTCTC	1 cut(s)	821
Eco32I	GATATC	1 cut(s)	1703
Eco47I	GGWCC	6 cut(s)	254, 632, 733, 986, 1684, 2105
Eco57I	CTGAAG	2 cut(s)	132, 183
Eco91I	GGTNACC	2 cut(s)	1555, 2042
EcoO109I	RGGNCCY	6 cut(s)	254, 510, 986, 1684, 2105, 2185
EcoO65I	GGTNACC	2 cut(s)	1555, 2042
EcoRII	CCWGG	4 cut(s)	64, 506, 1172, 1820
EcoRV	GATATC	1 cut(s)	1703
EcoT14I	CCWWGG	7 cut(s)	183, 831, 1149, 1162, 1402, 1587, 1687
EcoT22I	ATGCAT	3 cut(s)	1036, 2160, 2616
ErhI	CCWWGG	7 cut(s)	183, 831, 1149, 1162, 1402, 1587, 1687
FaqI	GGGAC	1 cut(s)	2238
FauI	CCCGC	5 cut(s)	1318, 1633, 2118, 2215, 2233
FauNDI	CATATG	2 cut(s)	840, 2023
FbaI	TGATCA	1 cut(s)	1467
FblI	GTMKAC	3 cut(s)	160, 726, 2205
FokI	GGATG	9 cut(s)	163, 209, 1043, 1236, 1341, 1355, 2052, 2454, 2574
FspBI	CTAG	4 cut(s)	143, 351, 693, 1403
GsuI	CTGGAG	4 cut(s)	288, 429, 1843, 2576
HapII	CCGG	7 cut(s)	60, 1228, 1652, 2140, 2282, 2406, 2422
HgaI	GACGC	2 cut(s)	1403, 2294
Hin1I	GRCGYC	1 cut(s)	2286
HincII	GTYRAC	3 cut(s)	277, 916, 2206
HindII	GTYRAC	3 cut(s)	277, 916, 2206
HindIII	AAGCTT	1 cut(s)	452
HinfI	GANTC	4 cut(s)	477, 844, 1119, 1258
HpaII	CCGG	7 cut(s)	60, 1228, 1652, 2140, 2282, 2406, 2422
HphI	GGTGA	8 cut(s)	369, 659, 1225, 1339, 1549, 1732, 2010, 2054
Hpy166II	GTNNAC	9 cut(s)	161, 277, 370, 727, 916, 1334, 1856, 1909, 2206
Hpy8I	GTNNAC	9 cut(s)	161, 277, 370, 727, 916, 1334, 1856, 1909, 2206
Hpy99I	CGWCG	2 cut(s)	2345, 2454
HpyAV	CCTTC	3 cut(s)	207, 1214, 1977
HpyCH4IV	ACGT	3 cut(s)	2121, 2331, 2601
HpyF3I	CTNAG	7 cut(s)	402, 591, 706, 810, 1889, 1901, 1994
HpySE526I	ACGT	3 cut(s)	2121, 2331, 2601
Hsp92I	GRCGYC	1 cut(s)	2286
Kpn2I	TCCGGA	1 cut(s)	2281
KroI	GCCGGC	1 cut(s)	1227
KroNI	GCCGGC	1 cut(s)	1229
Ksp22I	TGATCA	1 cut(s)	1467
Kzo9I	GATC	7 cut(s)	317, 329, 499, 1069, 1158, 1467, 2468
LmnI	GCTCC	6 cut(s)	910, 1444, 2128, 2569, 2595, 2605
Lsp1109I	GCAGC	9 cut(s)	151, 289, 428, 589, 979, 1384, 1489, 1923, 2248
MaeI	CTAG	4 cut(s)	143, 351, 693, 1403
MaeII	ACGT	3 cut(s)	2121, 2331, 2601
MalI	GATC	7 cut(s)	319, 331, 501, 1071, 1160, 1469, 2470
MboI	GATC	7 cut(s)	317, 329, 499, 1069, 1158, 1467, 2468
MboII	GAAGA	4 cut(s)	125, 2204, 2463, 2518
MflI	RGATCY	3 cut(s)	317, 1158, 2468
MhlI	GDGCHC	5 cut(s)	372, 811, 1137, 1911, 2133
MlsI	TGGCCA	1 cut(s)	1692
MluNI	TGGCCA	1 cut(s)	1692
MlyI	GAGTC	1 cut(s)	838
MmeI	TCCRAC	3 cut(s)	527, 2364, 2432
Mox20I	TGGCCA	1 cut(s)	1692
Mph1103I	ATGCAT	3 cut(s)	1036, 2160, 2616
MroI	TCCGGA	1 cut(s)	2281
MroNI	GCCGGC	1 cut(s)	1227
MroXI	GAANNNNTTC	1 cut(s)	1304
MscI	TGGCCA	1 cut(s)	1692
MslI	CAYNNNNRTG	6 cut(s)	362, 1266, 1497, 1539, 1676, 2377
Msp20I	TGGCCA	1 cut(s)	1692
MspA1I	CMGCKG	3 cut(s)	419, 602, 1915
MspCI	CTTAAG	1 cut(s)	1103
MspI	CCGG	7 cut(s)	60, 1228, 1652, 2140, 2282, 2406, 2422
MspR9I	CCNGG	6 cut(s)	61, 66, 508, 1174, 1822, 2407
Mva1269I	GAATGC	1 cut(s)	2616
MvaI	CCWGG	4 cut(s)	66, 508, 1174, 1822
NaeI	GCCGGC	1 cut(s)	1229
NciI	CCSGG	2 cut(s)	61, 2407
NcoI	CCATGG	3 cut(s)	183, 831, 1149
NdeI	CATATG	2 cut(s)	840, 2023
NdeII	GATC	7 cut(s)	317, 329, 499, 1069, 1158, 1467, 2468
NgoMIV	GCCGGC	1 cut(s)	1227
NheI	GCTAGC	1 cut(s)	142
NlaIV	GGNNCC	9 cut(s)	220, 512, 987, 1160, 1186, 1755, 2186, 2447, 2571
NmeAIII	GCCGAG	1 cut(s)	1741
NmuCI	GTSAC	7 cut(s)	1240, 1345, 1555, 1625, 2042, 2056, 2434
NsiI	ATGCAT	3 cut(s)	1036, 2160, 2616
NspI	RCATGY	2 cut(s)	539, 1633
OliI	CACNNNNGTG	1 cut(s)	362
PagI	TCATGA	2 cut(s)	739, 1569
PciI	ACATGT	1 cut(s)	535
PcsI	WCGNNNNNNNCGW	1 cut(s)	2337
PctI	GAATGC	1 cut(s)	2616
PdiI	GCCGGC	1 cut(s)	1229
PdmI	GAANNNNTTC	1 cut(s)	1304
PfeI	GAWTC	3 cut(s)	477, 1119, 1258
PflFI	GACNNNGTC	1 cut(s)	1343
PfoI	TCCNGGA	1 cut(s)	2405
PleI	GAGTC	1 cut(s)	838
PpsI	GAGTC	1 cut(s)	838
Ppu21I	YACGTR	1 cut(s)	2602
PpuMI	RGGWCCY	4 cut(s)	254, 986, 1684, 2105
PscI	ACATGT	1 cut(s)	535
PshBI	ATTAAT	1 cut(s)	294
PsiI	TTATAA	1 cut(s)	2321
Psp5II	RGGWCCY	4 cut(s)	254, 986, 1684, 2105
Psp6I	CCWGG	4 cut(s)	64, 506, 1172, 1820
PspEI	GGTNACC	2 cut(s)	1555, 2042
PspGI	CCWGG	4 cut(s)	64, 506, 1172, 1820
PspN4I	GGNNCC	9 cut(s)	220, 512, 987, 1160, 1186, 1755, 2186, 2447, 2571
PspPPI	RGGWCCY	4 cut(s)	254, 986, 1684, 2105
PstI	CTGCAG	2 cut(s)	997, 1941
PstNI	CAGNNNCTG	3 cut(s)	590, 2108, 2500
PsuI	RGATCY	3 cut(s)	317, 1158, 2468
PsyI	GACNNNGTC	1 cut(s)	1343
PvuII	CAGCTG	2 cut(s)	419, 1915
RsaI	GTAC	1 cut(s)	2347
RsaNI	GTAC	1 cut(s)	2346
RseI	CAYNNNNRTG	6 cut(s)	362, 1266, 1497, 1539, 1676, 2377
SalI	GTCGAC	1 cut(s)	2204
Sau3AI	GATC	7 cut(s)	317, 329, 499, 1069, 1158, 1467, 2468
SchI	GAGTC	1 cut(s)	838
ScrFI	CCNGG	6 cut(s)	61, 66, 508, 1174, 1822, 2407
SduI	GDGCHC	5 cut(s)	372, 811, 1137, 1911, 2133
SfcI	CTRYAG	2 cut(s)	993, 1937
SinI	GGWCC	6 cut(s)	254, 632, 733, 986, 1684, 2105
SmiMI	CAYNNNNRTG	6 cut(s)	362, 1266, 1497, 1539, 1676, 2377
SmlI	CTYRAG	2 cut(s)	1103, 1706
SmoI	CTYRAG	2 cut(s)	1103, 1706
SsiI	CCGC	7 cut(s)	600, 1108, 1325, 1640, 2125, 2222, 2240
SspMI	CTAG	4 cut(s)	143, 351, 693, 1403
StyD4I	CCNGG	6 cut(s)	59, 64, 506, 1172, 1820, 2405
StyI	CCWWGG	7 cut(s)	183, 831, 1149, 1162, 1402, 1587, 1687
TaiI	ACGT	3 cut(s)	2124, 2334, 2604
TaqI	TCGA	2 cut(s)	1845, 2205
TauI	GCSGC	1 cut(s)	1111
TfiI	GAWTC	3 cut(s)	477, 1119, 1258
TscAI	CASTG	8 cut(s)	43, 811, 940, 1465, 1700, 1897, 2496, 2536
TseFI	GTSAC	7 cut(s)	1240, 1345, 1555, 1625, 2042, 2056, 2434
TseI	GCWGC	9 cut(s)	139, 302, 416, 602, 992, 1397, 1502, 1936, 2261
Tsp45I	GTSAC	7 cut(s)	1240, 1345, 1555, 1625, 2042, 2056, 2434
TspDTI	ATGAA	7 cut(s)	87, 132, 775, 938, 1250, 1313, 1751
TspGWI	ACGGA	2 cut(s)	713, 1066
TspRI	CASTG	8 cut(s)	43, 811, 940, 1465, 1700, 1897, 2496, 2536
Tth111I	GACNNNGTC	1 cut(s)	1343
Vha464I	CTTAAG	1 cut(s)	1103
VneI	GTGCAC	2 cut(s)	368, 1907
VpaK11BI	GGWCC	6 cut(s)	254, 632, 733, 986, 1684, 2105
VspI	ATTAAT	1 cut(s)	294
XapI	RAATTY	3 cut(s)	94, 424, 1050
XceI	RCATGY	2 cut(s)	539, 1633
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1379
XmaJI	CCTAGG	1 cut(s)	1402
XmiI	GTMKAC	3 cut(s)	160, 726, 2205
XmnI	GAANNNNTTC	1 cut(s)	1304
XspI	CTAG	4 cut(s)	143, 351, 693, 1403
Zsp2I	ATGCAT	3 cut(s)	1036, 2160, 2616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.