Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0005339

Physical Location & Seq

Genomic Coordinates Forward (+)

64885647 .. 64889818

Gene Structure 4172 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00272940.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGGGGATGCTTAGTTTGCCAAAAAACACTGTGGTTCGTTCCACCGTGGGAGTCTCCAGTGTCAAGTCATCAAATGTGAAGCAGATAAACAGAGAGCATTTCACTAGTGTGCATCCAAAGCCATCTACTGAGAGAGCAGCAGCTTCAGAGCGTACGAGTAATCATCAGGGATCAAAGGGAAATGTTGATGAATCCAGTTCTGTTAGAGGTCTAAATCATGTTCCATATAACAGGACAGAAAACTCAGCAGCGGCAAACAGTGTTCAGACTCCAGAAACTCATGATAGAAGGGCTTTGCCACGAAAATCAAAAGCTCAGCCAAACCACTTCGTTCCAGATTTCAGTTCCAATGTACAGATTTCAGATGCAGAGACTACTAGATGTGGCCCTAAAGGTTTCAATAAACCTGTAAGAGAGATGAAAATGCCAACAGGGATTGCTCCATTCAACAGGCAGTGTGCGCACAAGGGGAATGTTGTTCAAAATGTTTCTCACATACTGCAACAGCTTAAATGGGGCCCTTCTGCAGAGGCAGCTCTTGGAAGTCTCAACTGTTCAATGGACGCATATCAAGCCAACCAAATTTTGAAGCAAATTCAGGATCATTCTGTTGCACTTGGATTTTTTAATTGGTTAAAACGACAAGCAGGATTCAGGCATGATGGGCACACTTACACCACCATGGTTGGCATCCTTGGTCGTGCCAGGCAGTTCGGCGCCATCAATAAATTACTTAATCAGATGGTGAATGAAGGATGTCAACCAAATGTTGTGACATACAATCGTCTGATTCATAGTTATGGTCGCGCAAACTATTTGAAAGACGCAATGAATGTGTTCAGTCAAATGCAAGGAGCAGGTTGTGAGCCTGACCGCGTCACCTACTGTACACTCATTGACATCCATGCTAAATCTGGGTTTCTTGATGTCGCCCTTGGACTGTATGATAGGATGCAAGAGGCTGGTCTTTCGCCTGACACATTTACTTACAGTGTTATGATCAACTGCCTTGGGAAAGCTGGCCATTTAGCTGCAGCTCACCGGCTGTTCTGTGAGATGGTGGATCATGGTTGTGTTCCCAACCTGGTCACCTATAATATCATGATAGCTTTGCAGGCCAAAGCAAGGAACTATGAGACTGCTTTAAAGCTCTACCGTGACATGCAGGGTGCTGGATTTCAGCCAGATAAAGTGACTTATAGCATAGTAATGGAGGTGCTTGGCCATTGTGGATACCTTGAGGAGGCAGAAGCACTTTTTGGTGAAATGAAACGGAAGAATTGGGTACCTGATGAACCTGTTTATGGCCTTTTAGTTGATTTGTGGGGCAAGGCTGGTAATGTGCAAAAAGCCTGGGATTGGTATCAAGCAATGCTCCATGCTGGTTTGCGTCCTAATGTGCCTACTTGCAATTCTCTTCTCAGTGCGTTTCTTAGGGTGCACCGTTTGTCTGATGCCTATAACTTGTTGCAGAGCATGGTGGGCTTAGGTCTGAACCCTTCTTTGCAGACATATACCTTGCTTCTCAGTTGTTGCACAGAAGCACGATCACCATATGACATGGAATTTTGTTGTGAGCTCATGGCAGTCACACGCCATCCTGCTCATACATTCCTACTGTCTATGCCATCAGCAGGACCCGATGGCCAAAACGTGAGAGAACACATGAGCAGCTTCTTGGATCTGATACATAGTGAGGATAGAGAAAGCAAGAGGGGGCTTGTAGATGCAGTAGTGGATTTCCTTCATAAATCAGGGCTCAAGGAGGAGGCTGGTTCTGTCTGGGAGGTGGCTGCACAGAAGAATGTTTATCCAGACGCCGTCAAAGAGAAAGGCTCATGTTATTGGCTTATAAACCTACATGTTATGTCTGATGGTACTGCTGTGACAGCACTATCAAGGACACTTGCTTGGTTTCGCCAAGAAATGCTAATATCTGGGGTTTGTCCAAACCGGATTGATATTGTGACAGGATGGGGACGGCGGAGTAGGGTTACAGGAACTTCTATGGTGAGGCATGCAGTGCAGGAATTGCTGCACATGTTTAGCTTCCCGTTTTTCACTGAGAATGGGAATTCTGGGTGCTTTGTAGGATGTGGGGAGTCTCTTAATAGATGGTTACTTGAATCTTATGTGGAGCGGATGCATTTATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

718

Amino Acids

79.89

Weight (kDa)

8.49

Isoelectric Point (pI)

35.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	216 - 268	6.9e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	218 - 306	2.9e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	223 - 261	5e-08	PPR repeat family
PPR_3	PF13812	243 - 301	1.2e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	252 - 284	9e-11	PPR repeat
PPR_2	PF13041	255 - 303	3.3e-15	PPR repeat family
PPR	PF01535	258 - 288	2.4e-06	PPR repeat
PPR_3	PF13812	279 - 339	5.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	287 - 319	9.6e-07	PPR repeat
PPR	PF01535	293 - 323	6.9e-06	PPR repeat
PPR_2	PF13041	293 - 339	2.7e-11	PPR repeat family
PPR_long	PF17177	308 - 411	1.5e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	315 - 371	3.1e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	322 - 354	1.2e-08	PPR repeat
PPR	PF01535	328 - 358	1.4e-06	PPR repeat
PPR_2	PF13041	333 - 369	4.4e-11	PPR repeat family
PPR_3	PF13812	348 - 408	1.1e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	361 - 407	1.8e-12	PPR repeat family
PPR	PF01535	398 - 427	3.6e-06	PPR repeat
PPR_2	PF13041	430 - 477	2.1e-06	PPR repeat family
PPR_3	PF13812	458 - 512	6.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	465 - 512	3.9e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005339)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18900 AT1G18900 AT1G18900 AT1G18900
fragaria_vesca	FvH4_5g15730 FvH4_5g15730 FvH4_5g15730
malus_domestica	MD06G1185900.v1.1 MD14G1192100.v1.1
prunus_persica	Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1
pyrus_communis	pycom06g16530 pycom14g15980
rosa_chinensis	RchiOBHm_Chr7g0180381
rosa_laevigata	RLG00000005272
rosa_multiflora	Rmu_sc0003526.1_g000009
rosa_roxburghii	Rroxscaffold_3G00272940
rosa_rugosa	Rorug06G0432600 Rorug06G0432700 Rorug06G0432800 Rorug06G0432800
rosa_samantha	Rh7AG033200 Rh7BG033100 Rh7CG035000 Rh7DG033600
rosa_wichuraiana	Rw7G002770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1853
Acc16I	TGCGCA	1 cut(s)	462
Acc36I	ACCTGC	1 cut(s)	848
Acc65I	GGTACC	1 cut(s)	1285
AccB1I	GGYRCC	2 cut(s)	716, 1285
AccBSI	CCGCTC	1 cut(s)	2140
AccII	CGCG	2 cut(s)	807, 876
AciI	CCGC	4 cut(s)	251, 874, 1984, 2140
AclWI	GGATC	4 cut(s)	178, 609, 1071, 1689
AcoI	YGGCCR	3 cut(s)	1021, 1222, 1645
AcsI	RAATTY	4 cut(s)	582, 594, 1565, 2074
AcuI	CTGAAG	1 cut(s)	129
AcyI	GRCGYC	2 cut(s)	717, 1818
AfaI	GTAC	5 cut(s)	154, 354, 889, 1287, 1879
AfiI	CCNNNNNNNGG	5 cut(s)	1125, 1243, 1304, 1359, 1634
AflIII	ACRYGT	2 cut(s)	1861, 2040
AgsI	TTSAA	7 cut(s)	400, 448, 482, 558, 589, 820, 2126
AhlI	ACTAGT	1 cut(s)	104
AjnI	CCWGG	3 cut(s)	704, 1083, 1352
AjuI	GAANNNNNNNTTGG	2 cut(s)	1242, 1274
AleI	CACNNNNGTG	1 cut(s)	107
Alw21I	GWGCWC	2 cut(s)	1443, 1581
Alw26I	GTCTC	5 cut(s)	58, 365, 551, 1130, 2109
Alw44I	GTGCAC	1 cut(s)	1439
AlwI	GGATC	4 cut(s)	178, 609, 1071, 1689
AoxI	GGCC	7 cut(s)	385, 517, 1021, 1116, 1222, 1306, 1645
ApaI	GGGCCC	1 cut(s)	521
ApaLI	GTGCAC	1 cut(s)	1439
ApeKI	GCWGC	9 cut(s)	137, 140, 248, 533, 1031, 1034, 1671, 1793, 2035
ApoI	RAATTY	4 cut(s)	582, 594, 1565, 2074
Asp700I	GAANNNNTTC	1 cut(s)	836
Asp718I	GGTACC	1 cut(s)	1285
AspLEI	GCGC	3 cut(s)	463, 719, 809
AspS9I	GGNCC	4 cut(s)	386, 517, 518, 1637
AsuHPI	GGTGA	7 cut(s)	757, 871, 1031, 1081, 1274, 1542, 2023
AvaII	GGWCC	1 cut(s)	1637
BaeGI	GKGCMC	3 cut(s)	521, 669, 1443
BaeI	ACNNNNGTAYC	2 cut(s)	1869, 1902
BalI	TGGCCA	3 cut(s)	1023, 1224, 1647
BanI	GGYRCC	2 cut(s)	716, 1285
BanII	GRGCYC	3 cut(s)	521, 1581, 1761
Bbv12I	GWGCWC	2 cut(s)	1443, 1581
BbvI	GCAGC	9 cut(s)	149, 152, 260, 545, 1018, 1046, 1683, 1780, 2022
BceAI	ACGGC	2 cut(s)	1805, 1997
BciT130I	CCWGG	3 cut(s)	706, 1085, 1354
BciVI	GTATCC	1 cut(s)	1226
BclI	TGATCA	1 cut(s)	999
BcoDI	GTCTC	5 cut(s)	58, 365, 551, 1130, 2109
BcuI	ACTAGT	1 cut(s)	104
BfaI	CTAG	2 cut(s)	105, 378
BfmI	CTRYAG	2 cut(s)	525, 1032
BfoI	RGCGCY	1 cut(s)	720
BfuAI	ACCTGC	1 cut(s)	848
BfuI	GTATCC	1 cut(s)	1226
BlpI	GCTNAGC	1 cut(s)	315
Bme1390I	CCNGG	3 cut(s)	706, 1085, 1354
Bme18I	GGWCC	1 cut(s)	1637
BmgT120I	GGNCC	4 cut(s)	386, 517, 518, 1637
BmiI	GGNNCC	5 cut(s)	518, 519, 718, 1287, 1639
BmrFI	CCNGG	3 cut(s)	706, 1085, 1354
BmsI	GCATC	7 cut(s)	121, 355, 699, 942, 1444, 1717, 2133
BplI	GAGNNNNNCTC	2 cut(s)	1820, 1852
BpmI	CTGGAG	2 cut(s)	40, 255
Bpu10I	CCTNAGC	1 cut(s)	1486
Bpu1102I	GCTNAGC	1 cut(s)	315
BpuEI	CTTGAG	2 cut(s)	1259, 1745
BsaBI	GATNNNNATC	1 cut(s)	1362
BsaHI	GRCGYC	2 cut(s)	717, 1818
BsaJI	CCNNGG	6 cut(s)	45, 681, 694, 934, 1009, 1353
BsaWI	WCCGGW	1 cut(s)	1953
Bsc4I	CCNNNNNNNGG	5 cut(s)	1125, 1243, 1304, 1359, 1634
Bse118I	RCCGGY	1 cut(s)	1041
Bse1I	ACTGG	2 cut(s)	57, 195
Bse3DI	GCAATG	2 cut(s)	834, 1377
Bse8I	GATNNNNATC	1 cut(s)	1362
BseBI	CCWGG	3 cut(s)	706, 1085, 1354
BseDI	CCNNGG	6 cut(s)	45, 681, 694, 934, 1009, 1353
BseJI	GATNNNNATC	1 cut(s)	1362
BseLI	CCNNNNNNNGG	5 cut(s)	1125, 1243, 1304, 1359, 1634
BseMI	GCAATG	2 cut(s)	834, 1377
BseMII	CTCAG	6 cut(s)	120, 258, 329, 1435, 1540, 2055
BseNI	ACTGG	2 cut(s)	57, 195
BseRI	GAGGAG	2 cut(s)	1256, 1781
BseSI	GKGCMC	3 cut(s)	521, 669, 1443
BseXI	GCAGC	9 cut(s)	149, 152, 260, 545, 1018, 1046, 1683, 1780, 2022
BsgI	GTGCAG	3 cut(s)	1779, 2021, 2045
Bsh1236I	CGCG	2 cut(s)	807, 876
BshFI	GGCC	7 cut(s)	387, 519, 1023, 1118, 1224, 1308, 1647
BshNI	GGYRCC	2 cut(s)	716, 1285
BsiHKAI	GWGCWC	2 cut(s)	1443, 1581
BsiSI	CCGG	2 cut(s)	1042, 1954
BsiWI	CGTACG	1 cut(s)	152
BslFI	GGGAC	1 cut(s)	1992
BslI	CCNNNNNNNGG	5 cut(s)	1125, 1243, 1304, 1359, 1634
BsmAI	GTCTC	5 cut(s)	58, 365, 551, 1130, 2109
BsmFI	GGGAC	1 cut(s)	1992
BsnI	GGCC	7 cut(s)	387, 519, 1023, 1118, 1224, 1308, 1647
Bsp120I	GGGCCC	1 cut(s)	517
Bsp1286I	GDGCHC	5 cut(s)	521, 669, 1443, 1581, 1761
Bsp1407I	TGTACA	2 cut(s)	352, 887
Bsp143I	GATC	6 cut(s)	170, 601, 999, 1063, 1547, 1681
Bsp1720I	GCTNAGC	1 cut(s)	315
Bsp19I	CCATGG	1 cut(s)	681
BspACI	CCGC	4 cut(s)	251, 874, 1984, 2140
BspANI	GGCC	7 cut(s)	387, 519, 1023, 1118, 1224, 1308, 1647
BspCNI	CTCAG	6 cut(s)	121, 257, 328, 1434, 1539, 2056
BspFNI	CGCG	2 cut(s)	807, 876
BspHI	TCATGA	2 cut(s)	280, 1101
BspLI	GGNNCC	5 cut(s)	518, 519, 718, 1287, 1639
BspMAI	CTGCAG	2 cut(s)	529, 1036
BspMI	ACCTGC	1 cut(s)	848
BspPI	GGATC	4 cut(s)	178, 609, 1071, 1689
BspT107I	GGYRCC	2 cut(s)	716, 1285
BsrBI	CCGCTC	1 cut(s)	2140
BsrDI	GCAATG	2 cut(s)	834, 1377
BsrFI	RCCGGY	1 cut(s)	1041
BsrGI	TGTACA	2 cut(s)	352, 887
BsrI	ACTGG	2 cut(s)	57, 195
BssAI	RCCGGY	1 cut(s)	1041
BssECI	CCNNGG	6 cut(s)	45, 681, 694, 934, 1009, 1353
BssMI	GATC	6 cut(s)	170, 601, 999, 1063, 1547, 1681
BssNI	GRCGYC	2 cut(s)	717, 1818
BssT1I	CCWWGG	4 cut(s)	681, 694, 934, 1009
Bst2UI	CCWGG	3 cut(s)	706, 1085, 1354
Bst6I	CTCTTC	1 cut(s)	1422
BstACI	GRCGYC	2 cut(s)	717, 1818
BstAPI	GCANNNNNTGC	2 cut(s)	2023, 2032
BstAUI	TGTACA	2 cut(s)	352, 887
BstC8I	GCNNGC	3 cut(s)	1021, 1116, 2019
BstDEI	CTNAG	9 cut(s)	11, 129, 244, 315, 1421, 1433, 1486, 1526, 2064
BstDSI	CCRYGG	2 cut(s)	45, 681
BstEII	GGTNACC	1 cut(s)	1087
BstENI	CCTNNNNNAGG	1 cut(s)	1241
BstFNI	CGCG	2 cut(s)	807, 876
BstH2I	RGCGCY	1 cut(s)	720
BstHHI	GCGC	3 cut(s)	463, 719, 809
BstKTI	GATC	6 cut(s)	173, 604, 1002, 1066, 1550, 1684
BstMAI	GTCTC	5 cut(s)	58, 365, 551, 1130, 2109
BstMBI	GATC	6 cut(s)	170, 601, 999, 1063, 1547, 1681
BstNI	CCWGG	3 cut(s)	706, 1085, 1354
BstNSI	RCATGY	4 cut(s)	1165, 1865, 2021, 2044
BstPI	GGTNACC	1 cut(s)	1087
BstSCI	CCNGG	3 cut(s)	704, 1083, 1352
BstSFI	CTRYAG	2 cut(s)	525, 1032
BstSLI	GKGCMC	3 cut(s)	521, 669, 1443
BstUI	CGCG	2 cut(s)	807, 876
BstV1I	GCAGC	9 cut(s)	149, 152, 260, 545, 1018, 1046, 1683, 1780, 2022
BstX2I	RGATCY	1 cut(s)	1681
BstYI	RGATCY	1 cut(s)	1681
BsuI	GTATCC	1 cut(s)	1226
BsuRI	GGCC	7 cut(s)	387, 519, 1023, 1118, 1224, 1308, 1647
BtgI	CCRYGG	2 cut(s)	45, 681
BtsI	GCAGTG	2 cut(s)	461, 2028
BtsIMutI	CAGTG	8 cut(s)	27, 64, 265, 461, 997, 1429, 2028, 2061
BveI	ACCTGC	1 cut(s)	848
Cac8I	GCNNGC	3 cut(s)	1021, 1116, 2019
CciI	TCATGA	2 cut(s)	280, 1101
CfoI	GCGC	3 cut(s)	463, 719, 809
Cfr10I	RCCGGY	1 cut(s)	1041
Cfr13I	GGNCC	4 cut(s)	386, 517, 518, 1637
CseI	GACGC	5 cut(s)	572, 833, 865, 1379, 1826
CsiI	ACCWGGT	1 cut(s)	1083
Csp6I	GTAC	5 cut(s)	153, 353, 888, 1286, 1878
CspCI	CAANNNNNGTGG	2 cut(s)	667, 702
CviQI	GTAC	5 cut(s)	153, 353, 888, 1286, 1878
DdeI	CTNAG	9 cut(s)	11, 129, 244, 315, 1421, 1433, 1486, 1526, 2064
DinI	GGCGCC	1 cut(s)	718
DpnI	GATC	6 cut(s)	172, 603, 1001, 1065, 1549, 1683
DpnII	GATC	6 cut(s)	170, 601, 999, 1063, 1547, 1681
DraI	TTTAAA	1 cut(s)	1146
EaeI	YGGCCR	3 cut(s)	1021, 1222, 1645
Eam1104I	CTCTTC	1 cut(s)	1422
EarI	CTCTTC	1 cut(s)	1422
EciI	GGCGGA	1 cut(s)	1999
Ecl136II	GAGCTC	1 cut(s)	1579
Eco130I	CCWWGG	4 cut(s)	681, 694, 934, 1009
Eco24I	GRGCYC	3 cut(s)	521, 1581, 1761
Eco47I	GGWCC	1 cut(s)	1637
Eco53kI	GAGCTC	1 cut(s)	1579
Eco57I	CTGAAG	1 cut(s)	129
Eco91I	GGTNACC	1 cut(s)	1087
EcoICRI	GAGCTC	1 cut(s)	1579
EcoNI	CCTNNNNNAGG	1 cut(s)	1241
EcoO109I	RGGNCCY	3 cut(s)	517, 518, 1637
EcoO65I	GGTNACC	1 cut(s)	1087
EcoRI	GAATTC	1 cut(s)	2074
EcoRII	CCWGG	3 cut(s)	704, 1083, 1352
EcoT14I	CCWWGG	4 cut(s)	681, 694, 934, 1009
EcoT22I	ATGCAT	1 cut(s)	2148
EcoT38I	GRGCYC	3 cut(s)	521, 1581, 1761
EgeI	GGCGCC	1 cut(s)	718
EheI	GGCGCC	1 cut(s)	718
ErhI	CCWWGG	4 cut(s)	681, 694, 934, 1009
FaqI	GGGAC	1 cut(s)	1992
FauNDI	CATATG	1 cut(s)	1555
FbaI	TGATCA	1 cut(s)	999
FokI	GGATG	9 cut(s)	19, 99, 677, 768, 887, 964, 1584, 1986, 2106
FriOI	GRGCYC	3 cut(s)	521, 1581, 1761
FspAI	RTGCGCAY	1 cut(s)	462
FspBI	CTAG	2 cut(s)	105, 378
FspI	TGCGCA	1 cut(s)	462
GlaI	GCGC	3 cut(s)	462, 718, 808
GsuI	CTGGAG	2 cut(s)	40, 255
HaeII	RGCGCY	1 cut(s)	720
HaeIII	GGCC	7 cut(s)	387, 519, 1023, 1118, 1224, 1308, 1647
HapII	CCGG	2 cut(s)	1042, 1954
HgaI	GACGC	5 cut(s)	572, 833, 865, 1379, 1826
HhaI	GCGC	3 cut(s)	463, 719, 809
Hin1I	GRCGYC	2 cut(s)	717, 1818
Hin6I	GCGC	3 cut(s)	461, 717, 807
HinP1I	GCGC	3 cut(s)	461, 717, 807
HincII	GTYRAC	1 cut(s)	761
HindII	GTYRAC	1 cut(s)	761
HinfI	GANTC	7 cut(s)	51, 191, 268, 651, 790, 2102, 2126
HpaII	CCGG	2 cut(s)	1042, 1954
HphI	GGTGA	7 cut(s)	757, 871, 1031, 1081, 1274, 1542, 2023
Hpy166II	GTNNAC	3 cut(s)	761, 890, 1441
Hpy188I	TCNGA	9 cut(s)	148, 267, 364, 741, 789, 1453, 1494, 1686, 1873
Hpy188III	TCNNGA	7 cut(s)	272, 281, 335, 599, 923, 1102, 1814
Hpy8I	GTNNAC	3 cut(s)	761, 890, 1441
HpyAV	CCTTC	5 cut(s)	282, 531, 746, 1509, 1754
HpyCH4IV	ACGT	1 cut(s)	1653
HpyF3I	CTNAG	9 cut(s)	11, 129, 244, 315, 1421, 1433, 1486, 1526, 2064
HpySE526I	ACGT	1 cut(s)	1653
Hsp92I	GRCGYC	2 cut(s)	717, 1818
HspAI	GCGC	3 cut(s)	461, 717, 807
KasI	GGCGCC	1 cut(s)	716
KpnI	GGTACC	1 cut(s)	1289
Ksp22I	TGATCA	1 cut(s)	999
Kzo9I	GATC	6 cut(s)	170, 601, 999, 1063, 1547, 1681
LmnI	GCTCC	4 cut(s)	445, 854, 1380, 2137
Lsp1109I	GCAGC	9 cut(s)	149, 152, 260, 545, 1018, 1046, 1683, 1780, 2022
LweI	GCATC	7 cut(s)	121, 355, 699, 942, 1444, 1717, 2133
MabI	ACCWGGT	1 cut(s)	1083
MaeI	CTAG	2 cut(s)	105, 378
MaeII	ACGT	1 cut(s)	1653
MalI	GATC	6 cut(s)	172, 603, 1001, 1065, 1549, 1683
MbiI	CCGCTC	1 cut(s)	2140
MboI	GATC	6 cut(s)	170, 601, 999, 1063, 1547, 1681
MboII	GAAGA	3 cut(s)	1288, 1409, 1813
MflI	RGATCY	1 cut(s)	1681
MhlI	GDGCHC	5 cut(s)	521, 669, 1443, 1581, 1761
MlsI	TGGCCA	3 cut(s)	1023, 1224, 1647
MluCI	AATT	9 cut(s)	582, 594, 628, 728, 1279, 1411, 1565, 2030, 2074
MluNI	TGGCCA	3 cut(s)	1023, 1224, 1647
Mly113I	GGCGCC	1 cut(s)	717
MlyI	GAGTC	3 cut(s)	60, 262, 2111
Mox20I	TGGCCA	3 cut(s)	1023, 1224, 1647
Mph1103I	ATGCAT	1 cut(s)	2148
MroXI	GAANNNNTTC	1 cut(s)	836
MscI	TGGCCA	3 cut(s)	1023, 1224, 1647
MseI	TTAA	6 cut(s)	510, 627, 635, 735, 1145, 2109
MslI	CAYNNNNRTG	5 cut(s)	107, 680, 798, 1071, 1208
Msp20I	TGGCCA	3 cut(s)	1023, 1224, 1647
MspA1I	CMGCKG	1 cut(s)	251
MspI	CCGG	2 cut(s)	1042, 1954
MspR9I	CCNGG	3 cut(s)	706, 1085, 1354
MvaI	CCWGG	3 cut(s)	706, 1085, 1354
MvnI	CGCG	2 cut(s)	807, 876
NarI	GGCGCC	1 cut(s)	717
NcoI	CCATGG	1 cut(s)	681
NdeI	CATATG	1 cut(s)	1555
NdeII	GATC	6 cut(s)	170, 601, 999, 1063, 1547, 1681
NlaIV	GGNNCC	5 cut(s)	518, 519, 718, 1287, 1639
NmuCI	GTSAC	8 cut(s)	772, 877, 1087, 1157, 1192, 1588, 1885, 1966
NsbI	TGCGCA	1 cut(s)	462
NsiI	ATGCAT	1 cut(s)	2148
NspI	RCATGY	4 cut(s)	1165, 1865, 2021, 2044
OliI	CACNNNNGTG	1 cut(s)	107
PaeI	GCATGC	1 cut(s)	2021
PagI	TCATGA	2 cut(s)	280, 1101
PciI	ACATGT	2 cut(s)	1861, 2040
PdmI	GAANNNNTTC	1 cut(s)	836
PfeI	GAWTC	4 cut(s)	191, 651, 790, 2126
Pfl23II	CGTACG	1 cut(s)	152
PflFI	GACNNNGTC	2 cut(s)	875, 1820
PleI	GAGTC	3 cut(s)	59, 262, 2110
PluTI	GGCGCC	1 cut(s)	720
PpsI	GAGTC	3 cut(s)	59, 262, 2110
PpuMI	RGGWCCY	1 cut(s)	1637
PscI	ACATGT	2 cut(s)	1861, 2040
PsiI	TTATAA	1 cut(s)	1853
Psp124BI	GAGCTC	1 cut(s)	1581
Psp5II	RGGWCCY	1 cut(s)	1637
Psp6I	CCWGG	3 cut(s)	704, 1083, 1352
PspEI	GGTNACC	1 cut(s)	1087
PspGI	CCWGG	3 cut(s)	704, 1083, 1352
PspLI	CGTACG	1 cut(s)	152
PspN4I	GGNNCC	5 cut(s)	518, 519, 718, 1287, 1639
PspOMI	GGGCCC	1 cut(s)	517
PspPI	GGNCC	4 cut(s)	386, 517, 518, 1637
PspPPI	RGGWCCY	1 cut(s)	1637
PstI	CTGCAG	2 cut(s)	529, 1036
PsuI	RGATCY	1 cut(s)	1681
PsyI	GACNNNGTC	2 cut(s)	875, 1820
RsaI	GTAC	5 cut(s)	154, 354, 889, 1287, 1879
RsaNI	GTAC	5 cut(s)	153, 353, 888, 1286, 1878
RseI	CAYNNNNRTG	5 cut(s)	107, 680, 798, 1071, 1208
SacI	GAGCTC	1 cut(s)	1581
SaqAI	TTAA	6 cut(s)	510, 627, 635, 735, 1145, 2109
Sau3AI	GATC	6 cut(s)	170, 601, 999, 1063, 1547, 1681
Sau96I	GGNCC	4 cut(s)	386, 517, 518, 1637
SchI	GAGTC	3 cut(s)	60, 262, 2111
ScrFI	CCNGG	3 cut(s)	706, 1085, 1354
SduI	GDGCHC	5 cut(s)	521, 669, 1443, 1581, 1761
SexAI	ACCWGGT	1 cut(s)	1083
SfaNI	GCATC	7 cut(s)	121, 355, 699, 942, 1444, 1717, 2133
SfcI	CTRYAG	2 cut(s)	525, 1032
SfoI	GGCGCC	1 cut(s)	718
SinI	GGWCC	1 cut(s)	1637
SmiMI	CAYNNNNRTG	5 cut(s)	107, 680, 798, 1071, 1208
SmlI	CTYRAG	2 cut(s)	1238, 1760
SmoI	CTYRAG	2 cut(s)	1238, 1760
SpeI	ACTAGT	1 cut(s)	104
SphI	GCATGC	1 cut(s)	2021
Sse9I	AATT	9 cut(s)	582, 594, 628, 728, 1279, 1411, 1565, 2030, 2074
SsiI	CCGC	4 cut(s)	251, 874, 1984, 2140
SspDI	GGCGCC	1 cut(s)	716
SspMI	CTAG	2 cut(s)	105, 378
SstI	GAGCTC	1 cut(s)	1581
StyD4I	CCNGG	3 cut(s)	704, 1083, 1352
StyI	CCWWGG	4 cut(s)	681, 694, 934, 1009
TaiI	ACGT	1 cut(s)	1656
TasI	AATT	9 cut(s)	582, 594, 628, 728, 1279, 1411, 1565, 2030, 2074
TatI	WGTACW	2 cut(s)	352, 887
TauI	GCSGC	1 cut(s)	254
TfiI	GAWTC	4 cut(s)	191, 651, 790, 2126
Tru1I	TTAA	6 cut(s)	510, 627, 635, 735, 1145, 2109
Tru9I	TTAA	6 cut(s)	510, 627, 635, 735, 1145, 2109
TscAI	CASTG	8 cut(s)	34, 64, 265, 461, 997, 1429, 2028, 2068
TseFI	GTSAC	8 cut(s)	772, 877, 1087, 1157, 1192, 1588, 1885, 1966
TseI	GCWGC	9 cut(s)	137, 140, 248, 533, 1031, 1034, 1671, 1793, 2035
Tsp45I	GTSAC	8 cut(s)	772, 877, 1087, 1157, 1192, 1588, 1885, 1966
TspDTI	ATGAA	8 cut(s)	204, 434, 765, 782, 845, 1283, 1308, 1736
TspGWI	ACGGA	1 cut(s)	1288
TspRI	CASTG	8 cut(s)	34, 64, 265, 461, 997, 1429, 2028, 2068
Tth111I	GACNNNGTC	2 cut(s)	875, 1820
VneI	GTGCAC	1 cut(s)	1439
VpaK11BI	GGWCC	1 cut(s)	1637
XagI	CCTNNNNNAGG	1 cut(s)	1241
XapI	RAATTY	4 cut(s)	582, 594, 1565, 2074
XceI	RCATGY	4 cut(s)	1165, 1865, 2021, 2044
XcmI	CCANNNNNNNNNTGG	1 cut(s)	911
XmnI	GAANNNNTTC	1 cut(s)	836
XspI	CTAG	2 cut(s)	105, 378
Zsp2I	ATGCAT	1 cut(s)	2148

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.